You searched for: Journal Theoretical and applied genetics Remove constraint Journal: Theoretical and applied genetics Source 1990 v.80 no.3 Remove constraint Source: 1990 v.80 no.3 Subject DNA Remove constraint Subject: DNA Subject restriction mapping Remove constraint Subject: restriction mapping ...
Rat kallikrein-binding protein is a novel serine-proteinase inhibitor that forms a covalent complex with tissue kallikrein. We have purified rat kallikrein-binding protein and cloned the cDNA and the gene encoding rat kallikrein-binding protein [Chao, Chai, Chen, Xiong, Chao, Woodley-Miller, Wang, Lu and Chao (1990) J. Biol. Chem. 265, 16394-16401; Chai, Ma, Murray, Chao and Chao (1991) J. Biol. Chem. 266, 16029-16036]. In the present study, we have expressed rat kallikrein-binding protein in Escherichia coli with a T7-polymerase/promoter expression system. A high level of expression was detected by an e.l.i.s.a. with an average of 24.2 mg of recombinant rat kallikrein-binding protein per 1 of culture. The recombinant protein appeared as a major protein in a crude extract of Escherichia coli on SDS/PAGE. It showed a molecular mass of 43 kDa and was recognized by polyclonal antibody to the native rat kallikrein-binding protein in Western-blot analysis. The recombinant rat kallikrein-binding ...
Linear) (Six-base) MAPSORT of: KIEE0945.seq Check: 8372 from: 1 to: 1050 KIEE0945.orf With 182 enzymes: SgfI * April 24, 2009 13:19 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn Cuts at: 0 162 271 645 1050 Size: 162 109 374 405 Fragments arranged by size: 405 374 162 109 AflIII ACryG_T Cuts at: 0 396 518 1050 Size: 396 122 532 Fragments arranged by size: 532 396 122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn Cuts at: 0 329 1050 Size: 329 721 AloI GAACnnnnnnTCCnnnnnnn_nnnnn Cuts at: 0 694 726 1050 Size: 694 32 324 Fragments arranged by size: 694 324 32 AlwNI CAG_nnnCTG Cuts at: 0 375 645 1050 Size: 375 270 405 Fragments arranged by size: 405 375 270 AseI ATTA_AT Cuts at: 0 566 1050 Size: 566 484 AvaI CyCGr_G Cuts at: 0 41 1050 Size: 41 1009 BanI GGyrC_C Cuts at: 0 48 1050 Size: 48 1002 BbsI GAAGACnnnnnn_ Cuts at: 0 634 1050 Size: 634 416 BciVI GTATCCnnnnn_n Cuts at: 0 999 1050 Size: 999 51 BclI TGATC_A Cuts at: 0 74 1050 Size: 74 976 BglI GCCn_nnnnGGC Cuts at: 0 590 1050 Size: 590 460 Bme1580I G_kGCmC Cuts ...
Linear) (Six-base) MAPSORT of: KIAA0030.seq Check: 2035 from: 1 to: 2712 KIAA0030.orf With 182 enzymes: SgfI * March 3, 2008 10:54 .. AarI CACCTGCnnnnnnnn_ Cuts at: 0 869 2517 2712 Size: 869 1648 195 Fragments arranged by size: 1648 869 195 AatII G_ACGTC Cuts at: 0 2372 2712 Size: 2372 340 AccI GTmk_AC Cuts at: 0 620 2712 Size: 620 2092 AcuI CTGAAGnnnnnnnnnnnnnn_nn Cuts at: 0 2179 2527 2640 2712 Size: 2179 348 113 72 Fragments arranged by size: 2179 348 113 72 AfeI AGCGCT Cuts at: 0 2100 2712 Size: 2100 612 AflIII ACryG_T Cuts at: 0 662 2123 2712 Size: 662 1461 589 Fragments arranged by size: 1461 662 589 AhdI GACnn_nnnGTC Cuts at: 0 320 2712 Size: 320 2392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn Cuts at: 0 2359 2712 Size: 2359 353 ApaI G_GGCCC Cuts at: 0 185 571 1725 2559 2712 Size: 185 386 1154 834 153 Fragments arranged by size: 1154 834 386 185 153 ApoI rAATT_y Cuts at: 0 1762 2712 Size: 1762 950 AvrII CCTAG_G Cuts at: 0 168 2712 Size: 168 2544 BaeI ACnnnnGTAyCnnnnnnn_nnnnn Cuts at: 0 ...
Cosmid.net - Mechelle Someones Knocking (Jul 15, 2014) 103 images totaling 74M Download set with the VG-Ripper MultiHosters.com K2S | RG | TF | UL
Cosmid.net - Sonny Twister Time With Sonny (Jul 25, 2014) 106 images totaling 63M Download set with the VG-Ripper MultiHosters.com K2S | RG | TF | UL
CiteWeb id: 19830000003. CiteWeb score: 27848. DOI: 10.1016/0003-2697(83)90418-9. A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described. DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers. Over 70% of the precursor triphosphate is routinely incorporated into complementary DNA, and specific activities of over 109 dpm/μg of DNA can be obtained using relatively small amounts of precursor. These oligolabeled DNA fragments serve as efficient probes in filter hybridization experiments.. Links: ...
Restriction mapping of HPV6BV cloned from Chinese women with 14 enzymes was established and compared with that of HPV6b from West Germany and different subtypes of HPV6 reported by foreign reseaehers to find the differences.
What I am confused by though, is the 1/2 and 1/4 component of the answer given. Can anyone give me a breakdown of that please ...
Restriction landmark genomic scanning (RLGS) has been used to study aberrant CpG island methylation in cancer for more than ten years. This approach remains one of the most reliable ways to characterize CpG island hypermethylation in cancer and has been used both to characterize differences in aberrant methylation phenotypes and also to identify tumor suppressor genes. Not only have known tumor suppressor genes like Cdkn2a (p16), Itga4 (α 4-integrin) [1], and Igfbp7 [2] been identified as targets of aberrant methylation in cancer by RLGS, but also novel tumor suppressor genes such as TCF21 [3], SLC5A8 [4], ID4 [5], BMP3B [6], and SOCS1 [7] have been identified by RLGS.. RLGS is a two-dimensional gel electrophoresis method [8] that allows detection of DNA methylation if a methylation sensitive landmark enzyme such as NotI is used. Up to 2,000 end-labeled landmark sites are displayed in a single RLGS profile. The labeling of the sites is based on incorporation of radionucleotides into the NotI ...
A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA. There are other ways of mapping features on DNA for longer length DNA molecules, such as mapping by transduction. One approach in constructing a restriction map of a DNA molecule is to sequence the whole molecule and to run the sequence through a computer program that will find the recognition sites that are present for every restriction enzyme known. Before sequencing was automated, it would have been prohibitively expensive to sequence an entire DNA strand. To find the relative positions of restriction sites on a plasmid, a technique involving single and double restriction digests is used. Based on the sizes of the resultant DNA fragments the positions of the sites can be inferred. Restriction ...
Plasmid pPSU1 from Dr. Song Tans lab contains the inserts 500 bp EcoRV fragment, 1000 bp EcoRV fragment, 1500 bp EcoRV fragment, 2000 bp EcoRV fragment, 500 bp PstI fragment, 700 bp PstI fragment, 800 bp PstI fragment, 900 bp PstI fragment, 1000 bp PstI fragment, and 2000 bp PstI fragment and is published in Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1. This plasmid is available through Addgene.
Purchase Recombinant Saccharopolyspora erythraea Alanine--tRNA ligase(alaS) ,partial. It is produced in Yeast. High purity. Good price.
Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder with a high penetrance characterized by tumors of the parathyroid glands, the endocrine pancreas, and the anterior pituitary. The MEN1 gene, a putative tumor suppressor gene, has been mapped to a 3- to 8-cM region in chromosome 11q13 but it remains elusive as yet. We have combined the efforts and resources from four laboratories to form the European Consortium on MEN1 with the aims of establishing the genetic and the physical maps of 11q13 and of further narrowing the MEN1 region. A 5-Mb integrated map of the region was established by fluorescence in situ hybridization on both metaphase chromosomes and DNA fibers, by hybridization to DNA from somatic cell hybrids containing various parts of human chromosome 11, by long-range restriction mapping, and by characterization of YACs and cosmids. Polymorphic markers were positioned and ordered by physical mapping and genetic linkage in 86 MEN1 families with 452 affected individuals.
Research groupsGene regulation and morphogenesis Genetics and functional genomics of genes with small open reading frames Dr Juan Pablo ..
Research groupsGene regulation and morphogenesis Genetics and functional genomics of genes with small open reading frames Dr Juan Pablo ..
Hi All, I am in trouble. I am developing an algorithm for restriction enzyme analysis but it is taking too long. The reason is because there are so many degenerate bases in the DNA sequence and thus it takes very long to analyze for all of them considering the possible combiantions they make. All the more the recognition sequence also have degenerate bases. Is there anybody out there to help me optimize the algorithm? Yes, there is. So thanking in advance to all those who respond. Ravi Gupta. Research Scholar DA University, M.P., India. -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own ...
Restriction Digest Protocol, Principle, result. restriction enzyme digestion. restriction digestion principle. restriction digest time
This chapter summarizes the recent findings of bacterial genomics and comments on the themes and trends which are emerging. A variety of techniques and methods are available to construct physical maps, and those most commonly employed involve pulsed field gel electrophoresis (PFGE) of macrorestriction fragments generated by digesting intact genomic DNA, immobilized in agarose plugs, with rare-cutting enzymes. Hybridization techniques are often used to construct a map and to deduce the positions of genetic markers. In recent years significant effort has been devoted to developing direct-mapping techniques for large DNA molecules that do not require gel electrophoresis. Among the more promising of these are two new methods known as DNA combing and optical mapping, both of which make use of fluorescence microscopy and image analysis to visualize single DNA molecules. Overall, bacterial genomes range in size from about 0.6 to 9.4 Mb. In a recent review, it was suggested that there may be a relationship
Genomic organization, chromosomal mapping, nucleotide sequence, and predicted amino acid sequence of the murine MCP-5 gene. (A) Partial restriction map and ge
The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions. The system is equipped with pulsed-field electrophoresis for resolving large DNA.. Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer. One sample is fractionated on a single precast agarose cassette, and one or two cassettes may be processed at one time.. Benefits of the SageELF System: ...
Thanks kashonal for asking..yes, the enzymes are ok. fortunately i was able to digest my plasmids now. However i wasnt able to figure out where my mistake was in my previous 2 tries ...
View Notes - lab Write-up from ENGLISH 1011 at Berkeley. Jacob Zipperstein H. Bio Med January 28, 2009 Title: DNA Restriction Analysis (Lambda DNA) Purpose: The purpose of this lab is to analyze
Restriction digestion troubleshooting - posted in Molecular Cloning: Hello all! I am trying to cut my vector with XhoI and SpeI. I setup a digestion reaction like this: 3.3 ul template (1ug) 2 ul 10x NEB Buffer 4 2 ul 10x BSA (I prepared this by diluting 100x stock in water, not 1X buffer) 1ul XhoI 1ul SpeI 11.7 ul H20 Incubation 37 C for 1h, inactivation 65 C for 20 min. Then I run on a gel the cut and uncut vector. In each lane, I got two top bands that were quite faint, defin...
Cosmid clones containing alpha 1-antitrypsin (alpha 1AT) gene sequences were observed to contain alpha 1AT-like sequences approximately 12 kb downstream of the authentic alpha 1AT gene. Restriction mapping suggested the alpha 1AT-like gene lacks promoter sequences. Cosmid clones from one library con …
A BioBrick is a sequence of DNA with a predefined structure and function. This payload is held in a circular plasmid, which is an isolated, circular piece of DNA that can replicate in bacteria. BioBricks™ are created with the intention of being easily joined and manipulated. In order for this to be possible, the BioBrick™ assembly standard requires the use of defined prefix and suffix sequences (flanking both sides of the BioBrick) that contain specific restriction endonuclease sites. These sites are called EcoRI, NotI and XbaI in the upstream, and SpeI, NotI, and PstI in the downstream. Naturally, the parts must also be engineered such that these sites are not present in the functional region of the sequence.[3] Cutting the BioBrick at specific restriction sites (using restriction enzymes) is what gives a BioBrick its interlocking ends. The end of one BioBrick can then be connected, or ligated, together with the end of another BioBrick, allowing you to effectively string together ...
A BioBrick is a sequence of DNA with a predefined structure and function. This payload is held in a circular plasmid, which is an isolated, circular piece of DNA that can replicate in bacteria. BioBricks™ are created with the intention of being easily joined and manipulated. In order for this to be possible, the BioBrick™ assembly standard requires the use of defined prefix and suffix sequences (flanking both sides of the BioBrick) that contain specific restriction endonuclease sites. These sites are called EcoRI, NotI and XbaI in the upstream, and SpeI, NotI, and PstI in the downstream. Naturally, the parts must also be engineered such that these sites are not present in the functional region of the sequence[3]. Cutting the BioBrick at specific restriction sites (using restriction enzymes) is what gives a BioBrick its interlocking ends. The end of one BioBrick can then be connected, or ligated, together with the end of another BioBrick, allowing you to effectively string together ...
Qui presentiamo lassemblaggio di chimera mediante il recupero del plasmide e linserimento del sito enzimatico di restrizione ...
Hier präsentieren wir die Chimären-Assemblierung durch Plasmid-Wiedergewinnung und Restriktionsenzym-Insertion (CAPRRESI), ein...
A genomic library is a collection of bacteria which have been genetically engineered to hold the entire DNA of an organism. This...
thanks a lot I understood little bit , but in my case as told to me that the ordered nucleotide sequence will be incoroporated with the plasmid and then i have to to subclone it into E.coli TOF competent cell and then i have to plate it select for the colonies and then do the mini prep to get the plasmid and then I have to do the pcr... I really didnt understand How can I do the pcr with the plasmid (Although my gene of intreset in there). but my question is if i do by this process and I do the pcr Should I get the band on 1,4kb AND THEN I EXCISE IT AND CARRYOUT MY FURTHER PROCESS: IF i am right kindly tell me or wrong please guide me with your answer. I would be highly obliged to you, I am unableto understnd this concept ...
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Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Determinants Of The 2 X-Gene Products Of Bovine Leukemia- ...
Wardell, JN, Stocks, SM, Thomas, CR and Bushell, ME (2002) Decreasing the hyphal branching rate of Saccharopolyspora erythraea NRRL 2338 leads to increased resistance to breakage and increased antibiotic production ...
(KudoZ) English to Polish translation of complete open reading frame of a cDNA molecule: otwarta ramka odczytu (cząsteczki cDNA) [Medical].
Plasmid Construction. The pGL3-promoter and pGL3-basic plasmids were purchased from Promega. The pL6 plasmid was generated by ligation of the 1.3-kb KpnI and XhoI (-1326 to +1; the translation start site was designated as +1) DNA fragment of mouse MOR into the polylinker sites of a promoterless luciferase vector, pGL3-basic (Promega, Madison, WI). The sequence of the insertion was confirmed by sequencing. The DNA fragment flanking the poly (A) site was generated by PCR from mouse genomic DNA with a pair of primers (sense, 5′-AATAGGCCGGCCGCATTAGGAGCATTGCTGAG-3′; antisense, 5′-ACGCGTCGACCCTAACTCTGGGATGGCAAG-3′; the underlined nucleotides indicate the overhanging restriction enzyme sites for FseI and SalI, respectively). The pL6PA and pGL3pPA plasmids were constructed by subcloning this DNA fragment after digestion with FseI and SalI into pL6 and pGL3-promoter plasmid, respectively. The pL6 and pGL3-promoter plasmids also have been digested by FseI and SalI, to replace the original SV40 ...
Edvotek. Analysis of Eco RI Cleavage Patterns of Lambda DNAThis experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. …
Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains.
Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
DNA restriction is a technique often useful in DNA fingerprinting. DNA restriction basically implies that there is a cut or cleavage of a particular DNA molecule.
Study Flashcards On restriction enzymes/plasmids at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
1 ATGCCTGGGG AGCAGATGGA CCCTACTGGA AGTCAGTTGG ATTCAGATTT CTCTCAGCAA 61 GATACTCCTT GCCTGATAAT TGAAGATTCT CAGCCTGAAA GCCAGGTTCT AGAGGATGAT 121 TCTGGTTCTC ACTTCAGTAT GCTATCTCGA CACCTTCCTA ATCTCCAGAC GCACAAAGAA 181 AATCCTGTGT TGGATGTTGT GTCCAATCCT GAACAAACAG CTGGAGAAGA ACGAGGAGAC 241 GGTAATAGTG GGTTCAATGA ACATTTGAAA GAAAACAAGG TTGCAGACCC TGTGGATTCT 301 TCTAACTTGG ACACATGTGG TTCCATCAGT CAGGTCATTG AGCAGTTACC TCAGCCAAAC 361 AGGACAAGCA GTGTTCTGGG AATGTCAGTG GAATCTGCTC CTGCTGTGGA GGAAGAGAAG 421 GGAGAAGAGT TGGAACAGAA GGAGAAAGAG AAGGAAGAAG ATACTTCAGG CAATACTACA 481 CATTCCCTTG GTGCTGAAGA TACTGCCTCA TCACAGTTGG GTTTTGGGGT TCTGGAACTC 541 TCCCAGAGCC AGGATGTTGA GGAAAATACT GTGCCATATG AAGTGGACAA AGAGCAGCTA 601 CAATCAGTAA CCACCAACTC TGGTTATACC AGGCTGTCTG ATGTGGATGC TAATACTGCA 661 ATTAAGCATG AAGAACAGTC CAACGAAGAT ATCCCCATAG CAGAACAGTC CAGCAAGGAC 721 ATCCCTGTGA CAGCACAGCC CAGTAAGGAT GTACATGTTG TAAAAGAGCA AAATCCACCA 781 CCTGCAAGGT CAGAGGACAT GCCTTTTAGC CCCAAAGCAT CTGTTGCTGC TATGGAAGCA 841 AAAGAACAGT TGTCTGCACA ...
Hoo boy, thats ugly. But it seems to work, and the tabs at the top are sorta nice - you can separate feeds by category. (Not sure Simpy plays nice with that - its supposed to, but…) Oh, and theres a minimum width otherwise you get side-scrolling, dont like that ...
You can take anything you find here, provided I made it myself and have not included it under someone elses terms, and do anything you want with it. You can do things I dont like, you can make money and not give me any, you can attribute the work to me or not, and you can tell me what youre up to or not, as you choose. You dont have to ask first ...
1 ATGGGAAAAA TCAGCAGTCT TCCAACCCAA TTATTTAAGT GCTGCTTTTG TGATTTCTTG 61 AAGGTGAAGA TGCACACCAT GTCCTCCTCG CATCTCTTCT ACCTGGCGCT GTGCCTGCTC 121 ACCTTCACCA GCTCTGCCAC GGCTGGACCG GAGACGCTCT GCGGGGCTGA GCTGGTGGAT 181 GCTCTTCAGT TCGTGTGTGG AGACAGGGGC TTTTATTTCA ACAAGCCCAC AGGGTATGGC 241 TCCAGCAGTC GGAGGGCGCC TCAGACAGGC ATCGTGGATG AGTGCTGCTT CCGGAGCTGT 301 GATCTAAGGA GGCTGGAGAT GTATTGCGCA CCCCTCAAGC CTGCCAAGTC AGCTCGCTCT 361 GTCCGTGCCC AGCGCCACAC CGACATGCCC AAGACCCAGA AGGAAGTACA TTTGAAGAAC 421 GCAAGTAGAG GGAGTGCAGG AAACAAGAAC TACAGGATGT AG ...
Rescue of the ABI3 coding region into a Gateway Entry vector. (A) Schematic diagram showing the location of primers and restriction enzyme sites used for analys
Gentaur molecular products has all kinds of products like :search , Gene Link \ Lambda gt10 reverse primer 24mer \ 26-3000-11 for more molecular products just contact us
In this study, 113 Enterococcus faecium, 37 Enterococcus faecalis, 24 Enterococcus gallinarum, 15 Enterococcus raffinosus, and 13 Enterococcus casseliflavus clinical isolates and American Type Culture Collection (ATCC) strains were evaluated by contour-clamped homogeneous electric field electrophoresis. Thirty-one of the E. faecium, 22 of the E. faecalis, 24 of the E. gallinarum, 15 of the E. raffinosus, and 13 of the E. casseliflavus isolates were also evaluated by DNA-DNA hybridization. Genomic DNAs from type strains E. faecalis ATCC 19433, E. faecium ATCC 19434, E. gallinarum ATCC 49573, E. raffinosus ATCC 49427, and E. casseliflavus ATCC 25788 were labeled with biotin for use as probes. E. faecalis differed from all other species in always having a largest fragment of , 400 kb. E. gallinarum was different from all other species in having all SmaI fragments of , 200 kb. Biotin-labeled probes showed a high degree of hybridization with genomic DNA from the same species and a low degree of ...
SUMMARY: Streptococcus mutans and Streptococcus sobrinus are the major causative organisms of human dental caries. Pulsed-field gel electrophoresis (PFG) showed that the restriction enzyme NotI produced ten and six DNA fragments from the genomes of S. mutans strain MT8148 and S. sobrinus strain 6715, respectively. The sizes of the chromosomes of S. mutans and S. sobrinus were each estimated to be about 2200 kb. The NotI restriction map of S. mutans MT8148 genome was constructed by Southern blot analysis with probes that overlapped two adjacent NotI fragments. Several virulence-associated genes of S. mutans were placed on the NotI restriction map. In addition, unique
Construction of a genomic DNA library with a TA vector - Genomic Library: cDNA Library: Definition: A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of
Use SimVector to draw plasmid maps, perform restriction analysis and mapping. The plasmid drawing software also simulates cloning experiments such as gateway cloning, ta cloning and restriction cloning
Shaun Tyler (styler at HWC.CA) wrote: : I=92m interested in sequencing a 40 kb plasmid (a native one not a clone) : for which I have a limited amount of material and no restriction map.=20 : Since what I have was hard to come by I=92m hesitant to waste it in : establishing a restriction map in order to subclone more manageable : fragments. Our sequencing capabilities are not a limiting factor so I : was thinking of using a shotgun approach with randomly sheared material : for a library and then closing the gaps by PCR and primer walking.=20 : Unfortunately I have been unable to find any protocols for preparing : mechanically sheared DNA. I know people occasionally use this method : for preparing genomic libraries but I=92m not sure how effective this : would be on something relatively small like a 40 kb plasmid. Any input : would be greatly appreciated. Paul Hengen (what would we do without him?) surveyed this in TIBS (1997) 22, 273-274. A nebulizing approach cited therein can be found close to ...
Molecular biology of nucleic acids and the techniques that form the basis of biotechnology. Topics include electrophoresis, restriction mapping, hybridization, plasmid analysis, and DNA cloning (recombinant DNA library construction, screening, and mapping ...
An important hurdle in analyzing interest rate targeting is that standard models usually lead to price level or inflation rate indeterminacy. This paper develops a simple framework in which such problems do not arise because the bonds whose interest rate is controlled provide liquidity services. This framework is used to examine interest rate targeting in a small open economy under predetermined exchange rates. A permanent increase in the interest rate has no real effects. In contrast, a temporary increase in the interest rate leads to higher consumption and to a current account deficit that worsens over time.
In the Toolbox you will find the Cloning and Restriction Sites tool that provides more control on the analysis, and gives you more output options such as a table of restriction sites. It also allows you to perform the same restriction map analysis on several sequences in one step ...
BamHI digest gives 7032 band (linearizes the plasmid). BamHI/NdeI double digest gives 6238 and 794 bands. Note - BamHI is susceptible to Star Activity. If digests looks smeary, this could be the culprit. New Engand Biolabs website has some guidelines for Star Activity. http: //www. neb. com/nebecomm/default. asp ...
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Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.