Bovine respiratory syncytial virus (BRSV) is the principal aetiological agent of the bovine respiratory disease complex. A BRSV subunit vaccine candidate consisting of two synthetic peptides representing putative protective epitopes on BRSV surface glycoproteins in soluble form or encapsulated in poly(lactide-co-glycolide) (PLG) microparticles were prepared. Calves (10 weeks old) with diminishing levels of BRSV-specific maternal antibody were intranasally administered a single dose of the different peptide formulations. Peptide-specific local immune responses (nasal secretion IgA), but not systemic humoral (serum IgG) or cellular responses (serum IFN-γ), were generated by all forms of peptide. There was a significant reduction in occurrence of respiratory disease in the animals inoculated with all peptide formulations compared to animals given PBS alone. Furthermore no adverse effects were observed in any of the animals post vaccination. These results suggest that intranasal immunisation with ...
Hägglund S, Hu K, Blodörn K, Makabi-Panzu B, Gaillard AL, Ellencrona K, Chevret D, Hellman L, Bengtsson KL, Riffault S, Taylor G, Valarcher JF, Eléouët JF Clin. Vaccine Immunol. 21 (7) 997-1004 [2014-07-00; online 2014-05-16] Bovine respiratory syncytial virus (BRSV) and human respiratory syncytial virus (HRSV) are major causes of respiratory disease in calves and children, respectively, and are priorities for vaccine development. We previously demonstrated that an experimental vaccine, BRSV-immunostimulating complex (ISCOM), is effective in calves with maternal antibodies. The present study focuses on the antigenic characterization of this vaccine for the design of new-generation subunit vaccines. The results of our study confirmed the presence of membrane glycoprotein (G), fusion glycoprotein (F), and nucleoprotein (N) proteins in the ISCOMs, and this knowledge was extended by the identification of matrix (M), M2-1, phosphoprotein (P), small hydrophobic protein (SH) and of cellular ...
Viuff, B, Uttenthal, A, Tegtmeier, C and Alexandersen, Soren 1996, Sites of replication of bovine respiratory syncytial virus in naturally infected calves as determined by in situ hybridization, Vetinary pathology, vol. 33, no. 4, pp. 383-390, doi: 10.1177/030098589603300403. ...
Reverse genetics for negative-strand RNA viruses, first developed for influenza virus (8, 22), has dramatically changed our understanding of the replication cycles of these viruses. In addition, this methodology has allowed genetic manipulation of viral genomes in order to generate new viruses, which can be used as live, attenuated vaccines or vectors to express heterologous proteins (12). The past 5 years have witnessed the rescue of most of the important nonsegmented, negative-strand RNA viruses from recombinant DNA. First, Schnell et al. (30) succeeded in the recovery of rabies virus from cloned DNA. Shortly after, rescue systems were developed for vesicular stomatitis virus (21, 32), respiratory syncytial virus (5, 18a), measles virus (29), Sendai virus (14, 20), and more recently for human parainfluenza type 3 (7, 17), rinderpest virus (1), simian virus 5 (16), bovine respiratory syncytial virus (4), and Newcastle disease virus (27). Bridgen and Elliott (3) succeeded in rescuing a ...
Reverse genetics for negative-strand RNA viruses, first developed for influenza virus (8, 22), has dramatically changed our understanding of the replication cycles of these viruses. In addition, this methodology has allowed genetic manipulation of viral genomes in order to generate new viruses, which can be used as live, attenuated vaccines or vectors to express heterologous proteins (12). The past 5 years have witnessed the rescue of most of the important nonsegmented, negative-strand RNA viruses from recombinant DNA. First, Schnell et al. (30) succeeded in the recovery of rabies virus from cloned DNA. Shortly after, rescue systems were developed for vesicular stomatitis virus (21, 32), respiratory syncytial virus (5, 18a), measles virus (29), Sendai virus (14, 20), and more recently for human parainfluenza type 3 (7, 17), rinderpest virus (1), simian virus 5 (16), bovine respiratory syncytial virus (4), and Newcastle disease virus (27). Bridgen and Elliott (3) succeeded in rescuing a ...
ONLINE COVER Moving Forward with an RSV Vaccine. Calves are natural hosts for bovine respiratory syncytial virus (RSV) infection. In people, RSV causes a severe lower respiratory tract disease that affects both children and the elderly. A pair of papers by Taylor et al. and Green et al. translate a prime-boost vaccine strategy for human RSV first into calves and then into humans in a phase 1 clinical trial. [CREDIT: TREASUREPHOTO/THINKSTOCK] ...
Calf bovine serum is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Blood is collected from donor animals by venipuncture between the ages of 16 and 22 weeks. Calf bovine serum is supplemented with iron to off-set the high concentrations of transferrin produced by formula-fed animals prior to collection. Sterility tests are performed on each lot of calf bovine serum using current USP and EP methods. Each lot is also checked for its ability to support the growth of cells. Specific testing for contaminants is listed below in the Specification Certificate. Origin This calf bovine serum is manufactured from calf bovine blood collected in USDA-inspected abattoirs located in the United States. Test* Specification Sterility Testing (Current USP and EP) Bacteria and Fungi No growth Virus Testing (9 CFR 113.53) Fluorescent antibody Bluetongue Bovine Adenovirus Bovine Parvovirus Bovine Respiratory Syncytial Virus Bovine Diarrhea Virus Rabies Reovirus
Calf bovine serum, iron fortified is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Blood is collected from donor animals by venipuncture between the ages of 16 and 22 weeks. Calf bovine serum is supplemented with iron to off-set the high concentrations of transferrin produced by formula-fed animals prior to collection. Sterility tests are performed on each lot of calf bovine serum using current USP and EP methods. Each lot is also checked for its ability to support the growth of cells. Specific testing for contaminants is listed below in the Specification Certificate. Origin This calf bovine serum is manufactured from calf bovine blood collected in USDA-inspected abattoirs located in the United States. Test* Specification Sterility Testing (Current USP and EP) Bacteria and Fungi No growth Virus Testing (9 CFR 113.53) Fluorescent antibody Bluetongue Bovine Adenovirus Bovine Parvovirus Bovine Respiratory Syncytial Virus Bovine Diarrhea
Human and bovine respiratory syncytial viruses (HRSV and BRSV) are two closely related, worldwide prevalent viruses that are the leading cause of severe airway disease in children and calves, respectively. Efficacy of commercial bovine vaccines needs improvement and no human vaccine is licensed yet. We reported that nasal vaccination with the HRSV nucleoprotein produced as recombinant ringshaped nanoparticles (NSRS) protects mice against a viral challenge with HRSV. The aim of this work was to evaluate this new vaccine that uses a conserved viral antigen, in calves, natural hosts for BRSV. Calves, free of colostral or natural anti-BRSV antibodies, were vaccinated with NSRS either intramuscularly, or both intramuscularly and intranasally using MontanideTM ISA71 and IMS4132 as adjuvants and challenged with BRSV. All vaccinated calves developed anti-N antibodies in blood and nasal secretions and N-specific cellular immunity in local lymph nodes. Clinical monitoring post-challenge demonstrated ...
Citation: Leach, R.J., Glass, E.J., Kuehn, L.A. 2012. Discovering regions of the bovine genome associated with variation in the immune response [abstract]. Journal of Animal Science Supplement. 90 (Supplement 2):24 (Abstract No. 62). Interpretive Summary: Technical Abstract: Infectious disease of livestock continues to be a cause of substantial economic loss and adverse welfare. Breeding for disease resistant livestock could improve both the economic burden and animal welfare. Using genetic linkage and association methods, we aim to identify key genes and pathways that control variation in immune response; knowledge that may aid both breeders and vaccinologists. The second generation of the RoBoGen herd (a Charolais Holstein F2 backcross, n = 982) were phenotyped for immune responses (total IgG responses) to a Bovine Respiratory Syncytial Virus (BRSV) vaccine (n = 467) across several time points. Considerable variance in immune responses enabled detection of QTL using 165 equally spaced ...
They determined their molecular beacon techniques deliver high-sensitivity and high-specificity results in both bovine and human RSV strains. "The RSV genome is interesting in that it is 15,000 nucleotides long, and one of its RNA sequences repeats itself nine times," Santangelo explained. "So we were able to bind up to nine probes to that sequence, and that helped us achieve very high sensitivity to the virus. In the human virus, in fact, we were able to see a single RSV virion.". Also, researchers were able to detect virion aggregates in bovine RSV within the first day in culture, Santangelo noted. Typically, veterinarians cannot detect RSV until after five or six days of incubation. Bovine RSV can be a major problem in cows, which represent a good animal model for human RSV. Calves have RSV symptoms similar to those in human babies, and the disease pathology is similar. So studying bovine RSV yields information about the strain that infects humans, he added. Also in this study, researchers ...
Viruses can be devastating, especially among domestic herd animals and companion animals like kennel-bound dogs that share close quarters.
8. Ellis, J., Gow, S., West, K., Waldner, C., Rhodes, C., Mutwiri, G., and Rosenberg, H.; Response of calves to challenge esposure with virulent bovine respiratory syncytial virus following intranasal administration of vaccines formulated for parenteral administraction. 2007. JAVMA. 230, 2:233-244 ...
This was a period when I skipped a lot of new releases in order to explore the classics of the past. I dont regret this, but the list provided an opportunity for me to play catch-up in an interesting way. I was already renting and watching movies on the list which I hadnt seen (and reviewing some of these) so I decided to turn this exercise into a regular series. I would watch any films from the top 100 which I hadnt already seen, and review them ...
Bovine respiratory disease (BRD) is the most important cause of clinical disease and death in feedlot cattle. Respiratory viral infections are key components in predisposing cattle to the development of this disease. To quantify the contribution of four viruses commonly associated with BRD, a case-control study was conducted nested within the National Bovine Respiratory Disease Initiative project population in Australian feedlot cattle. Effects of exposure to Bovine viral diarrhoea virus 1 (BVDV-1), Bovine herpesvirus 1 (BoHV-1), Bovine respiratory syncytial virus (BRSV) and Bovine parainfluenza virus 3 (BPIV-3), and to combinations of these viruses, were investigated.. Based on weighted seroprevalences at induction (when animals were enrolled and initial samples collected), the percentages of the project population estimated to be seropositive were 24% for BoHV-1, 69% for BVDV-1, 89% for BRSV and 91% for BPIV-3.. For each of the four viruses, seropositivity at induction was associated with ...
Define respiratory syncytial virus immune globulin intravenous. respiratory syncytial virus immune globulin intravenous synonyms, respiratory syncytial virus immune globulin intravenous pronunciation, respiratory syncytial virus immune globulin intravenous translation, English dictionary definition of respiratory syncytial virus immune globulin intravenous. n. 1. Any of a class of proteins that are widespread in blood plasma, milk, muscle, and plant seeds and that are insoluble in pure water but soluble in...
TY - JOUR. T1 - The secreted g protein of human respiratory syncytial virus antagonizes antibody-mediated restriction of replication involving macrophages and complement. AU - Bukreyev, Alexander. AU - Yang, Lijuan. AU - Collins, Peter L.. PY - 2012/10/1. Y1 - 2012/10/1. N2 - The respiratory syncytial virus (RSV) G and F glycoproteins are the neutralization antigens, and G also is expressed in a soluble form (sG). Previously, sG was demonstrated to reduce the efficiency of RSV antibody-mediated neutralization by serving as an antigen decoy and to inhibit the antibody-mediated antiviral effects of Fc receptor-bearing leukocytes. The present study demonstrated that effective antibody-mediated restriction in vivo, and the evasion of this restriction by sG, involves pulmonary macrophages and complement, but not neutrophils.. AB - The respiratory syncytial virus (RSV) G and F glycoproteins are the neutralization antigens, and G also is expressed in a soluble form (sG). Previously, sG was demonstrated ...
Several broad categories of patients are most vulnerable to RSV infection. These include:. premature infants and all infants less than 1 year of age, children 2 years old with cardiac disease or chronic lung disease (for example, asthma, cystic fibrosis, etc.), those of any age with a compromised immune system, and those 65 years of age or older. Is respiratory syncytial virus (RSV) contagious? Respiratory syncytial virus (RSV) is contagious. In the United States, its the most common cause of inflammation of the small airways in the lungs (bronchiolitis) and of pneumonia in children under 1 year of age. It also is significant cause of respiratory illnesses in older adults. Nearly all children in the U.S. will have been infected by RSV by 2 years of age. RSV usually causes a mild respiratory infection, but it can occasionally cause more serious infections that require hospitalization from breathing compromise with bronchiolitis or pneumonia. RSV was discovered in 1956 (isolated from a chimpanzee ...
The worldwide respiratory syncytial virus diagnostics market is poised to grow at a CAGR exceeding 10% over the forecast period (2016 to 2024). RSV Diagnostics Market stood at USD 625 million in 2015. High prevalence of neonatal infections & viral diseases and the need for early RSV detection mechanisms for infants are key drivers of this industry. Respiratory syncytial virus harms the respiratory tract, the immune system, the heart, and lungs; thus leading to serious illnesses. It mainly affects infants in the age group of 0 to 11 months.. However, there are instances of people above the age of 60 years being afflicted by this virus. This is essentially because of weak immunity. Some patients may even need hospitalization. Clinical symptoms of the RSV infection can seldom be distinguished from the symptoms of other respiratory disorders. Hence, there is a pressing need to develop precise & accurate diagnostic solutions for such a disease.. Browse Details of Report @ ...
The worldwide respiratory syncytial virus diagnostics market is poised to grow at a CAGR exceeding 10% over the forecast period (2016 to 2024). RSV Diagnostics Market stood at USD 625 million in 2015. High prevalence of neonatal infections & viral diseases and the need for early RSV detection mechanisms for infants are key drivers of this industry. Respiratory syncytial virus harms the respiratory tract, the immune system, the heart, and lungs; thus leading to serious illnesses. It mainly affects infants in the age group of 0 to 11 months.. However, there are instances of people above the age of 60 years being afflicted by this virus. This is essentially because of weak immunity. Some patients may even need hospitalization. Clinical symptoms of the RSV infection can seldom be distinguished from the symptoms of other respiratory disorders. Hence, there is a pressing need to develop precise & accurate diagnostic solutions for such a disease.. Browse Details of Report @ ...
The worldwide respiratory syncytial virus diagnostics market is poised to grow at a CAGR exceeding 10% over the forecast period (2016 to 2024). RSV Diagnostics Market stood at USD 625 million in 2015. High prevalence of neonatal infections & viral diseases and the need for early RSV detection mechanisms for infants are key drivers of this industry. Respiratory syncytial virus harms the respiratory tract, the immune system, the heart, and lungs; thus leading to serious illnesses. It mainly affects infants in the age group of 0 to 11 months.. However, there are instances of people above the age of 60 years being afflicted by this virus. This is essentially because of weak immunity. Some patients may even need hospitalization. Clinical symptoms of the RSV infection can seldom be distinguished from the symptoms of other respiratory disorders. Hence, there is a pressing need to develop precise & accurate diagnostic solutions for such a disease.. Browse Details of Report @ ...
Human respiratory syncytial virus (hRSV) is the leading cause of infant hospitalization related to respiratory disease. Infection with hRSV produces abundant infiltration of immune cells into the airways, which combined with an exacerbated pro-inflammatory immune response can lead to significant damage to the lungs. Human RSV re-infection is extremely frequent, suggesting that this virus may have evolved molecular mechanisms that interfere with host adaptive immunity. Infection with hRSV can be reduced by administering a humanized neutralizing antibody against the virus fusion protein in high-risk infants. Although neutralizing antibodies against hRSV effectively block the infection of airway epithelial cells, here we show that both, bone marrow-derived dendritic cells (DCs) and lung DCs undergo infection with IgG-coated virus (hRSV-IC), albeit abortive. Yet, this is enough to negatively modulate DC function. We observed that such a process is mediated by Fcγ receptors (FcγRs) expressed on the surface
Clinical trial for Respiratory syncytial virus , A Study to Evaluate the Safety Reactogenicity and Immunogenicity of Adenovirus Serotype 26 Based Respiratory Syncytial Virus Pre-fusion (Ad26.RSV.Pre-F) Vaccine in RSV-Seronegative Toddlers 12 to 24 Months of Age
Human RSV IgA ELISA kit is intended for determining in-vitro quantitative levels of human IgA antibodies against respiratory syncytial virus (RSV) in serum or
Summary Intergenic and flanking gene regions for the 1C-1B, 1B-N, N-P, M-1A, G-F and F-22K gene junctions of respiratory syncytial virus strain 18537, representing antigenic subgroup B, were determined by dideoxynucleotide sequencing of polycistronic cDNAs and mRNAs. Comparison with their counterparts from the subgroup A strain A2 showed that the intergenic sequences were not conserved within or between the strains. Flanking non-coding gene sequences also were generally not conserved except for the highly conserved gene-start and gene-end transcription signals. The sequence of the overlap between the 22K and L genes was conserved almost exactly between the two subgroups.
Respiratory Syncytial Virus (RSV) Fusion Protein antibody [681] for ELISA, ICC/IF. Anti-Respiratory Syncytial Virus (RSV) Fusion Protein mAb (GTX39259) is tested in Respiratory Syncytial Virus samples. 100% Ab-Assurance.
Respiratory syncytial virus (RSV) is a common virus that can have varying effects ranging from mild cold-like symptoms to mortality depending on the age and immune status of the individual. We combined mathematical modeling using ordinary differential equations (ODEs) with measurement of RSV infection kinetics in primary well differentiated human airway epithelial (HAE) cultures in vitro and in immunocompetent and immunosuppressed cotton rats to glean mechanistic details that underlie RSV infection kinetics in the lung. Quantitative analysis of viral titer kinetics in our mathematical model showed that the elimination of infected cells by the adaptive immune response generates unique RSV titer kinetic features including a faster time scale of viral titer clearance than viral production, and a monotonic decrease in the peak RSV titer with decreasing inoculum dose. Parameter estimation in the ODE model using a non-linear mixed effects approach revealed a very low rate (average single cell lifetime ...
(2004) Arnold et al. Virology. Respiratory syncytial virus (RSV) is worldwide the single most important respiratory pathogen in infancy and early childhood. The G glycoprotein of RSV, named attachment protein, is produced by RSV-infected lung epithelial cells in both a membrane-anchored (mG prote...
antibody-antibodies.com is the marketplace for research antibodies. Find the right antibody for your research needs. Respiratory syncytial virus (RSV) entry is inhibited by serine protease inhibitor AEBSF when present during an early stage of infection.
TechnoVax, a biotechnology developer of novel vaccines, has been awarded an NIH SBIR Grant to Develop a VLP based Respiratory Syncytial Virus (RSV) Va
respiratory syncytial virus M2-2 protein: involved in regulation of viral RNA transcription and replication, critical for virus replication; has been sequenced
Gentaur molecular products has all kinds of products like :search , AIC \ Respiratory Syncytial Virus, Antibody \ 5-RSV for more molecular products just contact us
Explore the problems and consequences of antibiotic resistant Respiratory Syncytial Virus (RSV). Learn how our rapid and reliable diagnostic tests can help!
NFID provides resources to educate healthcare professionals about the burden of respiratory syncytial virus (RSV), a common cause of acute respiratory illness in older adults, with the risk of serious infection increasing with age.
An experimental vaccine to protect against respiratory syncytial virus (RSV), a leading cause of illness and hospitalization among very young children, elicited high levels of RSV-specific antibodies when tested in animals, according to a report in the journal Science.
We have previously shown that respiratory syncytial virus (RSV) assembly occurs within regions of the host-cell surface membrane that are enriched in the protein caveolin-1 (cav-1). In this report, we have employed immunofluorescence microscopy to further examine the RSV assembly process. Our results show that RSV matures at regions of the cell surface that, in addition to cav-1, are enriched in the lipid-raft ganglioside GM1. Furthermore, a comparison of mock-infected and RSV-infected cells by confocal microscopy revealed a significant change in the cellular distribution of phosphocaveolin-1 (pcav-1). In mock-infected cells, pcav-1 was located at regions of the cell that interact with the extracellular matrix, termed focal adhesions (FA). In contrast, RSV-infected cells showed both a decrease in the levels of pcav-1 associated with FA and the appearance of pcav-1-containing cytoplasmic vesicles, the latter being absent in mock-infected cells. These cytoplasmic vesicles were clearly visible between 9
Respiratory syncytial virus enters the body through the eyes, nose or mouth (the three major openings in the body). It spreads easily and it is mostly airborne.
... (RSV) is a major cause of respiratory illness in young children. Learn how to recognize the signs and symptoms of this contagious infection.
... (RSV) is a major cause of respiratory illness in young children. Learn how to recognize the signs and symptoms of this contagious infection.
ContextInfluenza and respiratory syncytial virus (RSV) cause substantial morbidity and mortality. Statistical methods used to estimate deaths in the United Sta
Signs of respiratory syncytial virus in babies are similar to those of a cold, such as a runny nose and a cough, and they last a week or two, according to WebMD. Symptoms that require medical care...
The Public Health Laboratory Network have developed a standard case definition for the diagnosis of diseases which are notifiable in Australia. This page contains the laboratory case definition for respiratory syncytial virus.
Learn more about Respiratory Syncytial Virus at Grand Strand Medical Center DefinitionCausesRisk FactorsSymptomsDiagnosisTreatmentPreventionrevision ...
The report presents a detailed analysis of the Respiratory Syncytial Virus diagnostics market in the US, Europe, (France, Germany, Italy, Spain, UK) and Japan. ...
The worldwide Respiratory Syncytial Virus Diagnostics Market is poised to grow at a CAGR exceeding 10% over the forecast period (2016 to 2024). It stood at USD 625 million in 2015. High prevalence of neonatal infections & viral diseases and the need for early RSV detection mechanisms for infants are key drivers of this industry. Respiratory syncytial virus harms the respiratory tract, the immune system, the heart, and lungs; thus leading to serious illnesses. It mainly affects infants in the age group of 0 to 11 months.. However, there are instances of people above the age of 60 years being afflicted by this virus. This is essentially because of weak immunity. Some patients may even need hospitalization. Clinical symptoms of the RSV infection can seldom be distinguished from the symptoms of other respiratory disorders. Hence, there is a pressing need to develop precise & accurate diagnostic solutions for such a disease.. The global RSV Diagnostics market is categorized on the basis of products, ...
Temporary Exclusion Criteria for All Participants:. The following are temporary or self-limiting conditions and, once resolved, the person may be enrolled, if otherwise eligible. If the period of temporary exclusion is more than 56 days for adults or more than 30 days for RSV-seronegative children, the person will need to be rescreened. If the period of temporary exclusion is more than 56 days for RSV-seropositive children, a pre-inoculation serum antibody will need to be collected.. ...
Temporary Exclusion Criteria for All Participants:. The following are temporary or self-limiting conditions and, once resolved, the person may be enrolled, if otherwise eligible. If the period of temporary exclusion is more than 56 days for adults or more than 30 days for RSV-seronegative children, the person will need to be rescreened. If the period of temporary exclusion is more than 56 days for RSV-seropositive children, a pre-inoculation serum antibody will need to be collected.. ...
RSV is the most common cause of respiratory infections in children. Most children are infected with RSV during the first year of life and almost all have been infected by age two. However, immunity is temporary and most people will contract it again as adults.
The usual incubation period is 2 to 4 days, followed by the onset of rhinitis; severity of illness progresses to a peak within 1 to 3 days.
Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. Laboratory mice are infectable with human RSV but only semi-permissive for viral replication. Different strains and sub-strains of virus have different properties in the mouse model. We therefore cloned and tested a range of variants of A2 strain, finding one that is especially good at infecting and causing disease in mice. Virus storage: must be stored at minus 80 degrees centigrade or below. Needs to be thawed and rapidly used (unstable at room temperature for about one hour). Licensees are permitted to grow up stocks of their own according to supplied protocols. Internal case number: 7013. ...