Multi-tilt EM tomogram of primary human small airway epithelial cell (SAEC) in interphase state. The tomogram refers to the SAEC in Figure 4 of the pu...
Small airway (airway diameter , 1-2 mm) inflammation is thought to play a critical role in the pathogenesis of asthma including airways hyper-responsiveness, spontaneous exacerbations of symptoms, and tissue remodeling [3]. Non-invasive markers of inflammation, such as NO gas in the exhaled breath, could assist in the management of airway inflammation, but the anatomical source remains unclear. Our study demonstrates that small airway epithelial cells can be differentiated at an air-liquid interface to express markers such as mucin and cilia. The differentiated epithelium produces a very small, but detectable, amount of NO gas at baseline. However, the production is significantly increased, due to the upregulation of iNOS, following exposure to soluble inflammatory mediators, most notably a combination of IL-1β, TNF-α and IFN-γ. As such, iNOS in the small airway epithelium is a probable source of NO in the exhaled breath of asthma.. Bronchioles are generally , 1 mm in diameter, are devoid of ...
Primary airway epithelial cell culture provides a valuable tool for studying cell differentiation, cell-cell interactions, and the role of immune system factors in asthma
TY - JOUR. T1 - 15-deoxy-Δ-Prostaglandin J 2 induces IL-8 and GM-CSF in a human airway epithelial cell line (NCI-H 292). AU - Chiba, Takahito. AU - Ueki, Shigeharu. AU - Ito, Wataru. AU - Kato, Hikari. AU - Takeda, Masahide. AU - Kayaba, Hiroyuki. AU - Furue, Masutaka. AU - Chihara, Junichi. PY - 2009/6/1. Y1 - 2009/6/1. N2 - Background: 15-Deoxy-Δ 12,14-prostaglandin J 2 (15d-PGJ 2), a major prostanoid metabolized from prostaglandin D 2 (PGD 2), plays an important role in various biological processes including inflammatory responses. 15d-PGJ 2 exerts its effects through two major receptors, chemoattractant receptor- homologous molecule expressed on Th2 cells (CRTH2) and peroxisome proliferator-activated receptor-γ (PPARγ). The 15d-PGJ 2/PPARγ system, in particular, regulates numerous biological processes including adipogenesis, apoptosis, and inflammation. Although our studies have shown that PGD 2 (metabolic precursor of 15d-PGJ 2) induces IL-8 and GM-CSF production, the role of 15d-PGJ 2 ...
TY - JOUR. T1 - Modification of gene expression of the small airway epithelium in response to cigarette smoking. AU - Harvey, Ben Gary. AU - Heguy, Adriana. AU - Leopold, Philip L.. AU - Carolan, Brendan J.. AU - Ferris, Barbara. AU - Crystal, Ronald. PY - 2007/1/1. Y1 - 2007/1/1. N2 - The earliest morphologic evidence of changes in the airways associated with chronic cigarette smoking is in the small airways. To help understand how smoking modifies small airway structure and function, we developed a strategy using fiberoptic bronchoscopy and brushing to sample the human small airway (10th-12th order) bronchial epithelium to assess gene expression (Affymetrix HG-U133A and HG-133 Plus 2.0 array) in phenotypically normal smokers (n = 16, 25 ± 7 pack-years) compared to matched nonsmokers (n = 17). Compared to samples from large (second to third order) bronchi, the small airway samples had a higher proportion of ciliated cells, but less basal, undifferentiated, and secretory cells, and contained ...
Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses ...
The Pulmonary Epithelium in Health and Disease, 1st Edition By David Proud * Publisher: Wiley * Number Of Pages: 460 * Publication Date: 2008-06-03 * ISBN-10 /
Specialty Areas: Normal Physiology of Airway Surface Liquids (ASL); ASL System Failure in CF and COPD. Research Focus:. Dr. Bouchers lab has focused on the normal physiology of airway surface liquids (ASL) and how this system fails in airways diseases, e.g., cystic fibrosis and COPD. The lab pursues a variety of investigations into the functions of airway epithelia in health and disease. In general terms, the lab focuses on five interrelated areas of research:. ...
Approaches to drug screening typically involve dramatic compromises in order to achieve high throughput and hold down costs. Examples include the use of immorta...
BACKGROUND: The ability to transform normal human cells into cancer cells with the introduction of defined genetic alterations is a valuable method for understanding the mechanisms of oncogenesis. Easy establishment of immortalized but non-transformed human cells from various tissues would facilitate these genetic analyses. RESULTS: We report here a simple, one-step immortalization method that involves retroviral vector mediated co-expression of the human telomerase protein and a shRNA targeting the CDKN2A gene locus. We demonstrate that this method could successfully immortalize human small airway epithelial cells while maintaining their chromosomal stability. We further showed that these cells retain p53 activity and can be transformed by the KRAS oncogene. CONCLUSIONS: Our method simplifies the immortalization process and is broadly applicable for establishing immortalized epithelial cell lines from primary human tissues for cancer research.
TY - JOUR. T1 - Effects of a novel nonantibiotic macrolide, EM900, on cytokine and mucin gene expression in a human airway epithelial cell line. AU - Otsu, Kazuya. AU - Ishinaga, Hajime. AU - Suzuki, Shinya. AU - Sugawara, Akihiro. AU - Sunazuka, Toshiaki. AU - Omura, Satoshi. AU - Jono, Hirofumi. AU - Takeuchi, Kazuhiko. PY - 2011/12. Y1 - 2011/12. N2 - Background/Aims: Long-term macrolide therapy is an effective treatment for chronic sinusitis and diffuse panbronchiolitis. However, long-term use of macrolides may promote the growth of drug-resistant bacteria; therefore, development of macrolides with no antibacterial action is desirable. A new erythromycin (EM) derivative, (8R,9S)- 8,9-dihydro-6,9-epoxy-8,9- anhydropseudoerythromycin A (EM900), does not possess antibacterial action. Methods: To determine whether EM900 induced a clinically relevant anti-inflammatory response and repressed mucin gene expression in cells derived from human airway epithelia, we assessed the effects of EM900 on ...
Nipah virus (NiV) is a zoonotic emerging pathogen that can cause severe and often fatal respiratory disease in humans. The pathogenesis of NiV infection of the human respiratory tract remains unknown. Reactive oxygen species (ROS) produced by airway epithelial cells in response to viral infections contribute to lung injury by inducing inflammation and oxidative stress; however, the role of ROS in NiV-induced respiratory disease is unknown. To investigate whether NiV induces oxidative stress in human respiratory epithelial cells, we used oxidative stress markers and monitored antioxidant gene expression. We also used ROS scavengers to assess their role in immune response modulation. Oxidative stress was confirmed in infected cells and correlated with the reduction in antioxidant enzyme gene expression. Infected cells treated by ROS scavengers resulted in a significant decrease of the (F2)-8-isoprostane marker, inflammatory responses and virus replication. In conclusion, ROS are induced during NiV
ATCC ® Normal Human Primary Small Airway Epithelial Cells, when grown in Airway Epithelial Cell Basal Media supplemented with Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040) components, provide an ideal cell system to propagate small airway epithelial cells in serum-free conditions. The cells are cryopreserved at the first passage to ensure the highest viability and plating efficiency. ATCC ® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.
Cigarettes vary in tobacco blend, filter ventilation, additives, and other physical and chemical properties, but little is known regarding potential differences in toxicity to a smokers airway epithelia. We compared changes in gene expression and cytokine production in primary normal human bronchia …
RvD3 and AT-RvD3 impact on airway epithelial cells and the resolution of inflammatory responses after acute lung injury in mice ...
This study demonstrates for the first time the effects of IL-1beta on the regulation of protein production as well as MUC2 gene transcription in cultured human airway epithelial cells. The effect of IL-1beta on the regulation of MUC2 protein was determined by flow cytometric analysis. The expression …
TY - JOUR. T1 - SUV39H1 reduction is implicated in abnormal inflammation in COPD. AU - Chen, Tzu Tao. AU - Wu, Sheng Ming. AU - Ho, Shu Chuan. AU - Chuang, Hsiao Chi. AU - Liu, Chien Ying. AU - Chan, Yao Fei. AU - Kuo, Lu Wei. AU - Feng, Po Hao. AU - Liu, Wen Te. AU - Chen, Kuan Yuan. AU - Hsiao, Ta Chih. AU - Juang, Jer Nan. AU - Lee, Kang Yun. PY - 2017/1/1. Y1 - 2017/1/1. N2 - Chronic obstructive pulmonary disease (COPD) is characterized by enhanced chronic inflammation in the airways, lung parenchyma, and circulation. We investigated whether SUV39H1, a histone methyltransferase, is causatively implicated in the abnormal inflammation observed in COPD. The SUV39H1 and H3K9me3 levels were reduced in peripheral blood mononuclear cells (PBMCs), primary human small airway epithelial cells (HSAEpCs) and lung tissues from COPD patients, which were correlated with poor lung function and the serum IL-8 and IL-6 levels. A specific SUV39H1 inhibitor, chaetocin, induced a distinct COPD panel of ...
TY - JOUR. T1 - Identification of a human airway epithelial cell subpopulation with altered biophysical, molecular, and metastatic properties. AU - Pagano, Paul C.. AU - Tran, Linh M.. AU - Bendris, Nawal. AU - OByrne, Sean. AU - Tse, Henry T.. AU - Sharma, Shivani. AU - Hoech, Jonathan W.. AU - Park, Stacy J.. AU - Liclican, Elvira L.. AU - Jing, Zhe. AU - Li, Rui. AU - Krysan, Kostyantyn. AU - Paul, Manash K.. AU - Fontebasso, Yari. AU - Larsen, Jill E.. AU - Hakimi, Shaina. AU - Seki, Atsuko. AU - Fishbein, Michael C.. AU - Gimzewski, James K.. AU - Di Carlo, Dino. AU - Minna, John D.. AU - Walser, Tonya C.. AU - Dubinett, Steven M.. N1 - Funding Information: These studies were supported by funding from the following sources: NIH/NCI#T32-CA009120-36 (S.M. Dubinett), NCI#U01CA152751 (S.M. Dubinett), NCI#1U01CA196408 (S.M. Dubinett), Department of Defense Congressionally Directed Medical Research Programs#LC130767 (S.M. Dubinett), Department of Veteran Affairs#2I01BX000359-05A1 (S.M. ...
Increased mucus production is a common cause of morbidity and mortality in inflammatory airway diseases, including asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis. However, the precise molecular mechanisms for pathogenic mucus production are largely undetermined. Accordingly, there are no specific and effective anti-mucus therapeutics. Here, we define a signaling pathway from chloride channel calcium-activated 1 (CLCA1) to MAPK13 that is responsible for IL-13-driven mucus production in human airway epithelial cells. The same pathway was also highly activated in the lungs of humans with excess mucus production due to COPD. We further validated the pathway by using structure-based drug design to develop a series of novel MAPK13 inhibitors with nanomolar potency that effectively reduced mucus production in human airway epithelial cells. These results uncover and validate a new pathway for regulating mucus production as well as a corresponding therapeutic approach to mucus ...
Activation of transcription factor IL-6 (NF-IL-6) and nuclear factor-kappaB (NF-kappaB) by lipid ozonation products is crucial to interleukin-8 gene expression in human airway epithelial cells. Ozone (O(3)) is a major component of smog and an inhaled toxicant to the lung. O(3) rapidly reacts with the airway epithelial cell membrane phospholipids to... ...
TY - JOUR. T1 - A chimeric type 2 adenovirus vector with a type 17 fiber enhances gene transfer to human airway epithelia. AU - Zabner, J.. AU - Chillón, M.. AU - Grunst, T.. AU - Moninger, T.O.. AU - Davidson, B.L.. AU - Gregory, R.. AU - Armentano, D.. PY - 1999/1/1. Y1 - 1999/1/1. M3 - Article. VL - 73. SP - 8689. EP - 8695. JO - Journal of Virology. JF - Journal of Virology. SN - 0022-538X. ER - ...
The cellular tropism of the SARS-CoV-2 virus affects several aspects of infection, such as the spread of the virus within and between hosts, tissue pathology, immune control mechanisms, and the response to treatment with promising antiviral drugs.
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The long-term and central goal of the Cystic Fibrosis Research Center at the University of Pittsburgh is to provide improved treatments, and ultimately a cure, for cystic fibrosis (CF). A collaborative effort by investigators in the Departments of Cell Biology and Physiology, Medicine, and Pediatrics, our strategy is to implement this goal using our strengths in the basic science of CFTR and epithelial ion transport and to translate our increasingly applied basic science knowledge to clinical investigations.. In order to achieve these goals we have established centers of excellence in three broad categories: Cell and Molecular Biology of CF, Infection and Inflammation in CF, and Clinical Studies in CF. These centers are summarized below:. Cell and Molecular Biology: The bulk of our research is conducted using primary human airway cell cultures established from CF and non-CF tissues that are made available by the more than 100 lung transplants that are performed annually at the University of ...
Primary culture of respiratory epithelial cells is useful to study the pathophysiology of respiratory diseases. air-interface. In addition, GSK2606414 irreversible inhibition confluence of the cultures was confirmed by trans epithelial resistance (Rte) (57??4????cm2 at day 10, 165??67????cm2 at day 14, 1312??281????cm2 at day 28, and 1563??86????cm2 at day 40). Therefore, the authors concluded that the BCi NS1.1 cell line accurately mimics the airway epithelium, and can be considered as a model to study the interactions with environmental stimuli, cytokines, cigarette smoke or as a target for assessing of pharmacologic agents. However, studies of such interactions with the environment or drugs require not only a 100? % confluent culture but also polarization. The authors examined the Rte but this parameter is not sufficient to make conclusions about the polarization status of the cell line. Additionally, the Rte was 57??4????cm2 (day 10) and 165??67????cm2 (day 14) [2C5]. Moreover, this cell ...
Meet us at our poster: The Use of a Long-shelf life Human Airway Epithelium (MucilAir™) for evaluating the Nasal and Bronchial drug delivery ...
Li, J., Patterson, M., Chew, W. L., Cho, S-H., Gilmour, I., Oliver, T., ... Liedtke, W. (2011). TRPV4-Mediated calcium-influx into human bronchial epithelia upon exposure to diesel exhaust particles. Environmental Health Perspectives, 119(6), 784 - 793. ...
TY - JOUR. T1 - Effect of cytokines on ICAM-1 and ZO-1 expression on human airway epithelial cells. AU - Shahana, Shahida. PY - 2005/9. Y1 - 2005/9. N2 - The presence of adhesion molecules on airway epithelial cells may be important in recruiting leukocytes to the epithelium. The study aimed at investigating the effects of interleukin (IL)-4, IL-8, IL-13 and interferon-gamma (IFN-gamma) on cell viability and intracellular adhesion molecule (ICAM)-1 and zonula occludens protein (ZO)-1 expression on cultured human basal and columnar airway epithelial cells. Cycloheximide (CHX) induced cell death in both cell lines. The cytokines IL-4, IL-8, IL-13 and IFN-gamma had only minor effects on cell proliferation in the columnar 16HBE14o-cells, and inhibited the effects of CHX on cell death. IFN-gamma increased ICAM-1 expression in both cell lines. Western blot analysis showed that CHX inhibited both ICAM-1 and ZO-1 expression in the basal cell line. A combination of IL-4 and IFN-gamma appeared to break ...
Free Online Library: Phosphorylation of p53 protein in A549 human pulmonary epithelial cells exposed to asbestos fibers. (Research). by Environmental Health Perspectives; Health, general Environmental issues
MatTeks Normal Human Bronchial Epithelial Cells (NHBE) provide an ideal serum-free culture system to study cell-cell and cell-matrix interactions, drug
TY - JOUR. T1 - Knockdown of NHERF1 enhances degradation of temperature rescued ΔF508 CFTR from the cell surface of human airway cells. AU - Kwon, Sang Ho. AU - Pollard, Harvey. AU - Guggino, William B.. N1 - Copyright: Copyright 2019 Elsevier B.V., All rights reserved.. PY - 2007. Y1 - 2007. N2 - ΔF508 CFTR can be functionally restored in the plasma membrane by exposure of the cell to lower temperature. However, restored ΔF508 CFTR has a much shorter half-life than normal. We studied whether NHERF1, which binds to the PDZ motif of CFTR, might be a critical mediator in the turnover of ΔF508 CFTR from the cell surface. We used RNAi to reduce the expression of NHERF1 in human airway epithelial cells. Knockdown of NHERF1 reversibly reduces surface expression of WT-CFTR without altering its total expression. As expected, temperature correction increased mature C band ΔF508 CFTR (rΔF508) but unexpectedly allowed immature B band of rΔF508 to traffic to the cell surface. Both surface and total ...
Goal of the present study is to investigate the specific cellular responses to nCeO2 and nFe2O3 in various lung cell types and develop an in vitro chronic exposure model to predict the potential fibrogenic and carcinogenic effects. Primary human lung fibroblasts were treated with nCeO2 (size dXRD = 17 nm, SSA = 61 m2/g) and direct stimulation of collagen production (a hallmark of fibrosis) was evaluated. In separate experiments, primary human small airway epithelial cells were exposed to a sub-lethal concentration (0.625 µg/cm2) of nCeO2 and nFe2O3 (size dXRD = 20 nm, SSA = 50 m2/g) for 6 weeks and their effects on cell transformation and invasion were evaluated. Our results showed new data that nCeO2 can induce a dose-dependent increase in collagen production by lung fibroblasts; nCeO2 can induce proliferation of lung epithelial cells as compared to vehicle-treated control and nFe2O3 induced neoplastic transformation of epithelial cells as determined by soft-agar colony formation assay and transwell
Lin, H., Li, H., Cho, H.-J., Bian, S., Roh, H.-J., Lee, M.-K., Kim, J. S., Chung, S.-J., Shim, C.-K. and Kim, D.-D. (2007), Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies. J. Pharm. Sci., 96: 341-350. doi: 10.1002/jps.20803 ...
Asthma is a heterogeneous syndrome that has been subdivided into physiologic phenotypes and molecular endotypes. The most specific phenotypic manifestation of asthma is indirect airway hyperresponsiveness (AHR), and a prominent molecular endotype is the presence of type 2 inflammation. The underlying basis for type 2 inflammation and its relationship to AHR are incompletely understood. We assessed the expression of type 2 cytokines in the airways of subjects with and without asthma who were extensively characterized for AHR. Using quantitative morphometry of the airway wall, we identified a shift in mast cells from the submucosa to the airway epithelium specifically associated with both type 2 inflammation and indirect AHR. Using ex vivo modeling of primary airway epithelial cells in organotypic coculture with mast cells, we show that epithelial-derived IL-33 uniquely induced type 2 cytokines in mast cells, which regulated the expression of epithelial IL33 in a feed-forward loop. This ...
3896 Purpose: To evaluate the oncogenic impact of p53 knockdown, mutant K-RASV12, mutant EGFR alone and their combination on tumorigenicity of a newly developed immortalized human bronchial epithelial cell line. Background: Molecular analysis of lung cancer has revealed several genetic and epigenetic alterations in the multistep pathogenesis of lung cancer. However, little is known about the relative importance of each individual alteration on the tumorigenic process. One approach is to use a model system in which the contribution of each genetic alteration to lung tumorigenesis can be assessed individually and combinatorially. We have developed an in vitro model system using normal human bronchial epithelial cells that overexpress Cyclin-dependent kinase 4 and human telomerase. Ectopic expression of these two genes enables the cells to bypass the growth inhibitory signals of the p16/Rb pathway and also replicative senescence induced by shortened telomeres. We manipulated this cell line (HBEC3), ...
Excessive mucus production has been linked to many of the pathological features of respiratory diseases including obstruction of the airways, decline in lung function, increased rates of mortality and increased infections. The mucins MUC5AC and MUC5B contribute to the viscoelastic properties of mucus and are found at elevated levels in the airways of individuals with chronic respiratory diseases. The Th2 cell cytokine interleukin-13 (IL-13) is known to regulate MUC5AC expression in goblet cells of the airways, though much less is known about the regulation of MUC5B expression. In a study to further understand the mediators of MUC5AC and MUC5B expression, NRG1b1 was identified as novel regulator of goblet cell formation in primary cultures of human bronchial epithelial cells (HBECs). NRG1b1 increased expression of MUCAC and MUC5B proteins in a time and dose-dependent fashion in HBEC cultures. NRG1b1-induced expression of MU5AC and MUC5B was shown to involve ErbB2 and ErbB3 receptors but not ...
Act1/CIKS is an intracellular protein that has been shown to play an important role in mediating IL-17A and IL-25 signaling effects. Recently, defects in Act1 function and/or expression has been implicated in inflammatory disease, such as psoriatic arthritis and atopic dermatitis. We have found that the modulation of Act1 expression levels in human airway epithelial cells changes the expression levels of some genes, in the absence of cytokine stimulation. RNAseq is a powerful new technique to quantitatively measure changes at the transcriptome level. Here we describe the use of RNAseq to globally analyze the effects of modulating Act1 expression in human airway epithelial cells. ...
In our study we demonstrate that the plasma levels of UTP are significantly elevated in patients with acute myocardial infarction. A combination of pharmacology and mRNA quantification indicates that UTP is probably acting via P2Y2 receptors in man, and via both P2Y2 and P2Y4 receptors on cardiomyocytes from mice. Our results indicate that UDP is a novel inotropic factor acting via P2Y6 receptors.. The first demonstration of UTP release used [3H]uridine-labeled endothelial cells. In the study, [3H]UTP release occurred in response to increased flow.27 Since the development of the first sensitive quantitative assay for UTP, its release has been measured from a variety of cells including platelets, leukocytes, primary airway epithelial cells, rat astrocytes, and several other cell lines.24 To our knowledge, we are now the first to quantify UTP levels in human plasma. UTP levels correlated significantly with ATP, indicating corelease of the nucleotides. The UTP levels were ≈1:10 of the ATP levels, ...
The airway epithelial cell core provides investigators with primary culture preparations of human and mouse airway epithelial cells. We routinely procure human tissues from lung transplantation donors and explanted lungs and surgical tissues, including those with lung disease. The core can also culture airway cells obtained by lung and nasal brushing or scraping. In collaboration with core users, Brody Lab members will establish cultures from mice or materials provided by the core users. Core users can be instructed on all methods for culture, manipulation and evaluation of preparations. Lead time of one month should be provided to allow for scheduling and the necessary period for cell growth. IRB permission may be required for some tissues and studies.. ...
Lung cancer is the most common cause of cancer-related deaths. Tobacco smoke exposure is the strongest aetiological factor associated with lung cancer. In this study, using serial analysis of gene expression (SAGE), we comprehensively examined the effect of active smoking by comparing the transcriptomes of clinical specimens obtained from current, former and never smokers, and identified genes showing both reversible and irreversible expression changes upon smoking cessation. Twenty-four SAGE profiles of the bronchial epithelium of eight current, twelve former and four never smokers were generated and analyzed. In total, 3,111,471 SAGE tags representing over 110 thousand potentially unique transcripts were generated, comprising the largest human SAGE study to date. We identified 1,733 constitutively expressed genes in current, former and never smoker transcriptomes. We have also identified both reversible and irreversible gene expression changes upon cessation of smoking; reversible changes were
DESCRIPTION (provided by applicant): The long-term goal of this grant project is to understand the roles and mechanisms of the airway epithelium in Th2-type immune responses. Human airways are constantly exposed to the products of environmental allergens and microbes. Airway inflammation in patients with asthma is characterized by increased production of Th2 cytokines. However, the immunological mechanisms to explain the relationship between the environmental exposure and Th2-type inflammation in asthma are poorly understood. IL-33 is a new and potent Th2-driving cytokine; it is constitutively produced and stored in the nucleus of airway epithelial cells. We recently found that exposure to common aeroallegens, such as Alternaria and cockroach, induces a rapid release of ATP from airway epithelial cells, produces a sustained increase in intracellular calcium concentration ([Ca2+]i), and stimulates IL-33 secretion. Importantly, suppressing expression of P2 purinergic receptors, either P2Y2R or ...
CD1d is a MHC I like molecule which presents glycolipid to natural killer T (NKT) cells, a group of cells with diverse but critical immune regulatory functions in the immune system. These cells are required for optimal defence against bacterial, viral, protozoan, and fungal infections, and control of immune-pathology and autoimmune diseases. CD1d is expressed on antigen presenting cells but also found on some non-haematopoietic cells. However, it has not been observed on bronchial epithelium, a site of active host defence in the lungs. Here, we identify for the first time, CD1D mRNA variants and CD1d protein expression on human bronchial epithelial cells, describe six alternatively spliced transcripts of this gene in these cells; and show that these variants are specific to epithelial cells. These findings provide the basis for investigations into a role for CD1d in lung mucosal immunity.
Objective: Excessive airway inflammation is seen in chronic obstructive pulmonary disease (COPD) patients experiencing acute exacerbations, which are ..
NL20 (ATCC CRL-2503) is an immortalized, nontumorigenic human bronchial epithelial cell line derived from normal bronchus taken from an accident victim at autopsy. The cell line was established by transfection with the origin of replication-defective SV40 large T plasmid, p129.
NL20 (ATCC CRL-2503) is an immortalized, nontumorigenic human bronchial epithelial cell line derived from normal bronchus taken from an accident victim at autopsy. The cell line was established by transfection with the origin of replication-defective SV40 large T plasmid, p129. NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line.
Sigma-Aldrich offers abstracts and full-text articles by [Phillip A Wages, Robert Silbajoris, Adam Speen, Luisa Brighton, Andres Henriquez, Haiyan Tong, Philip A Bromberg, Steven O Simmons, James M Samet].
Bronchial epithelium. Coloured scanning electron micrograph (SEM) of a cultured bronchial epithelial cell. The respiratory epithelium is composed of a mixed population of ciliated, nonciliated, and mucous-secreting cells from proximal to distal airways. In vitro models ( cell culture) using primary cells and cell lines are essential for understanding the function and pathophysiology of these cells in diseases such as asthma. Magnification: x 2000 when printed 10 centimetres wide. - Stock Image C022/6437
As part of a robust innate immune system, the cells of the airway epithelium secrete fluid and proteins to create the highly proteinaceous periciliary liquid (PCL). Many proteins present in the PCL have proposed antimicrobial functions, including two of the most abundant proteins, BPIFA1 (SPLUNC1) and BPIFB1 (LPLUNC1). The function of these two proteins in host defence is unresolved and we hypothesize that they interact with the respiratory pathogen, S. aureus, to limit infection.. Air-liquid interface (ALI) cultures of primary bronchial epithelial cells secrete many proteins present in the PCL, including BPIFA1 and BPIFB1. Pull down assays interacting cell secretions with S. aureus were used to visualise protein-bacterial interactions. Both BPIFA1 and BPIFB1 were shown interact strongly with S. aureus. Recombinant proteins generated in CHO cells exhibited similar binding to the endogenous proteins. Deglycosylation using PNGase F treatment prior to pull down assays highlighted that these ...
My research interests are to elucidate the cellular and molecular mechanisms by which environmental carcinogens cause lung cancer and to identify markers for the diagnosis, prognosis and treatment of this disease. We investigate genetic and epigenetic changes in oncogenes and tumor suppressor genes in specimens obtained from lung cancer patients and individuals at high risk for developing lung cancer. In addition, we apply new mouse models to investigate pathways of tobacco smoke carcinogen-mediated lung tumorigenesis. We would like to understand how airway epithelial cells are involved in the initiation and progression of lung tumors, and how they are influenced by environmental factors that increase lung cancer risk and how they respond to therapies. ...
Tamang DL, Pirzai W, Priebe GP, Traficante DC, Pier GB, Falck JR, Morisseau C, Hammock BD, McCormick BA, Gronert K, Hurley BP. Hepoxilin A(3) facilitates neutrophilic breach of lipoxygenase-expressing airway epithelial barriers. J Immunol. 2012 Nov 15; 189(10):4960-9 ...
In this study, we examined the expression of the antimicrobial RNase 7 in cultured airway epithelial cells. We demonstrated that undifferentiated S-PBEC, primarily consisting of BCs, but not mucociliary-differentiated ALI-PBEC, expressed RNase 7 upon stimulation with the respiratory pathogen NTHi. This was in marked contrast to other examined innate immune mediators that were induced by NTHi in both S-PBEC and ALI-PBEC. Furthermore, differentiated ALI-PBEC displayed a decreased baseline RNase 7 expression compared with S-PBEC after 2 wk of culturing in air-exposed conditions. Cigarette smoke exposure, which caused transient disruption of the epithelial barrier function, reinitiated RNase 7 expression in the remaining undifferentiated BCs in ALI-PBEC, which was demonstrated using confocal microscopy and isolation of luminal and basal cell fractions. Moreover, we showed that this induction required activation of EGFR and the downstream ERK1/2 signaling pathway and furthermore demonstrated that ...
Bluhmki, T., Bitzer, S., Gindele, J. A., Schruf, E., Kiechle, T., Webster, M., Schymeinsky, J., Ries, R., Gantner, F., Bischoff, D., Garnett, J. and Heilker, R. Development of a miniaturized 96-Transwell air-liquid interface human small airway epithelial model. Scientific Reports. 2020; 10(1). doi: 10.1038/s41598-020-69948-2. Read More ...
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1) The main issue with this paper is this: these cell lines, although originally human, are all immortalized cancer cell lines characterised by markedly different biological properties when compared to normal human cells of the same tissue/cell type. They cant be readily used a surrogates for normal human tissue/cell types. None were primary cells nor were any even from recently acquired tissue samples from biopsies etc. People have infected primary human airway epithelial cultures with hCoV-EMC - so this can be done successfully - , although it would be more difficult for other tissue types as these cultures havent been developed. Some of these cell lines used may by chance lack key viral repressors of infection present in normal primary cells, which could skew results from cell culture infection experiments. Plus, a human tissue is not just a single cell type - they are composed of diverse kinds of cells that could together behave much, much differently than cell lines in culture ...
Incidence of acute asthma, defined as the number of persons who develop asthma within a specific time period, is approximately % annually. Childhood asthma persists into adulthood in approximately 50% of cases. Those with symptoms persisting into the second decade of life usually have asthma throughout adulthood. Asthma prevalence is 6-10% (ie, million persons); one half of these cases are children (ie, 8-20% of all children). Overall, acute asthma represents about 2% of all ED visits Childhood asthma
Hackett, T.L., Shaheen, F., Johnson, A., Wadsworth, S., Pechkovsky, D.V., Jacoby, D.B., et al. (2008) Characterization of side population cells from human airway epithelium. Stem Cells, 26, 2576-2585.doi10.1634/stemcells.2008-0171
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Mycobacteria are antibiotic-resistant microbes that are often implicated in lung infections. To fight them, the body activates interferon and other immune proteins, but scientists werent sure how the process worked
A new collaborative study describes a way that lung tissue can regenerate after injury. The team found that lung tissue has more dexterity in repairing tis | Genetics And Genomics
RT-PCR for IL-8 (A), IL-6 (B), HOX1 (C), and COX2 (D) relative to GAPDH in unexposed NHBE cells. RNA for all proteins significantly changed with differentiation
K+ channels are expressed in a wide variety of cell types, including airway epithelial cells. The purpose of this study was to investigate the role of various Kv and KCa channels in cell proliferation and cell volume ...
The respiratory epithelium is lined by a tightly balanced fluid layer that allows normal O-2 and CO2 exchange and maintains surface tension and host defense ...