B lymphocyte-induced maturation protein-1 (Blimp-1) is a transcriptional repressor that plays an important role during plasmacytic differentiation and is expressed in normal and transformed plasma cells. We here investigated the importance of continuous Blimp-1 expression. We found that knockdown of Blimp-1 expression by lentiviral vector-delivered short hairpin RNA causes apoptosis in multiple myeloma cell lines and plasmacytoma cells$ indicating that continued expression of Blimp-1 is required for cell survival. ...
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TY - JOUR. T1 - Transcriptional repressor REST drives lineage stage-specific chromatin compaction at Ptch1 and increases AKT activation in a mouse model of medulloblastoma. AU - Dobson, Tara H.W.. AU - Tao, Rong Hua. AU - Swaminathan, Jyothishmathi. AU - Maegawa, Shinji. AU - Shaik, Shavali. AU - Bravo-Alegria, Javiera. AU - Sharma, Ajay. AU - Kennis, Bridget. AU - Yang, Yanwen. AU - Callegari, Keri. AU - Haltom, Amanda R.. AU - Taylor, Pete. AU - Kogiso, Mari. AU - Qi, Lin. AU - Khatua, Soumen. AU - Goldman, Stewart. AU - Lulla, Rishi R.. AU - Fangusaro, Jason. AU - MacDonald, Tobey J.. AU - Li, Xiao Nan. AU - Hawkins, Cynthia. AU - Rajaram, Veena. AU - Gopalakrishnan, Vidya. PY - 2019/1/22. Y1 - 2019/1/22. N2 - In medulloblastomas (MBs), the expression and activity of RE1-silencing transcription factor (REST) is increased in tumors driven by the sonic hedgehog (SHH) pathway, specifically the SHH- (children 3 to 16 years) and SHH- (infants) subgroups. Neuronal maturation is greater in SHH- than ...
Neuron-restrictive silencer factor regulates the N-methyl-D-aspartate receptor 2B subunit gene in basal and ethanol-induced gene expression in fetal cortical ne
Previous studies have identified the immunological functions of transcription factor B lymphocyte-induced maturation protein-1 (Blimp-1) in various adaptive immune cell types such as T and B lymphocytes. More recently, it has been shown that Blimp-1 extends its functional roles to dendritic cells (DCs) and macrophages, two cell types belonging to the innate immune system. The protein acts as a direct and indirect regulator of target genes by recruiting chromatin modification factors and by regulating microRNA expression, respectively. In DCs, Blimp-1 has been identified as one of the components involved in antigen presentation. Genome-wide association studies identified polymorphisms associated with multiple autoimmune diseases such as system lupus erythematosus, rheumatoid arthritis, and inflammatory bowel disease in PRDM1, the gene encoding Blimp-1 protein. In this review, we will discuss the immune regulatory functions of Blimp-1 in DCs with a main focus on the tolerogenic mechanisms of Blimp-1
An example of a repressor protein is the methionine repressor MetJ. MetJ interacts with DNA bases via a ribbon-helix-helix (RHH) motif.[2] MetJ is a homodimer consisting of two monomers, which each provides a beta ribbon and an alpha helix. Together, the beta ribbons of each monomer come together to form an antiparallel beta-sheet which binds to the DNA operator (Met box) in its major groove. Once bound, the MetJ dimer interacts with another MetJ dimer bound to the complementary strand of the operator via its alpha helices. AdoMet binds to a pocket in MetJ that does not overlap the site of DNA binding. The Met box has the sequence AGACGTCT which is a palindrome (it shows dyad symmetry) allowing the same sequence to be recognised on either strand of the DNA. The junction between C and G in the middle of the Met box contains a pyrimidine-purine step that becomes positively supercoiled forming a kink in the phosphodiester backbone. This is how the protein checks for the recognition site as it ...
Transcription repressor. Molecular model of the Tup 1 transcription repressor protein. Transcription repressors bind to specific sequences of DNA (deoxyribonucleic acid) and prevent the transcription (transfer) of genetic information from DNA to RNA (ribonucleic acid). - Stock Image F006/9316
Compaction and looping of the ~2.5-Mb Igh locus during V(D)J rearrangement is essential to allow all V(H) genes to be brought in proximity with D(H)-J(H) segments to create a diverse antibody repertoire, but the proteins directly responsible for this are unknown. Because CCCTC-binding factor (CTCF) has been demonstrated to be involved in long-range chromosomal interactions, we hypothesized that CTCF may promote the contraction of the Igh locus. ChIP sequencing was performed on pro-B cells, revealing colocalization of CTCF and Rad21 binding at ~60 sites throughout the V(H) region and 2 other sites within the Igh locus. These numerous CTCF/cohesin sites potentially form the bases of the multiloop rosette structures at the Igh locus that compact during Ig heavy chain rearrangement. To test whether CTCF was involved in locus compaction, we used 3D-FISH to measure compaction in pro-B cells transduced with CTCF shRNA retroviruses. Reduction of CTCF binding resulted in a decrease in Igh locus ...
A central feature of broad host range IncP-1 plasmids is the set of regulatory circuits that tightly control plasmid core functions under steady-state conditions. Cooperativity between KorB and either KorA or TrbA repressor proteins is a key element of these circuits and deletion analysis has implicated the conserved C-terminal domain of KorAand TrbAin this interaction. By NMR we show that KorA and KorB interact directly and identify KorA amino acids that are affected on KorB binding. Studies on mutants showed that tyrosine 84 (or phenylalanine, in some alleles) is dispensable for repressor activity but critical for the specific interaction with KorB in both in vivo reporter gene assays and in vitro electrophoretic mobility shift and co-purification assays. This confirms that direct and specific protein-protein interactions are responsible for the cooperativity observed between KorB and its corepressors and lays the basis for determining the ...
Loss of the chromatin remodeling ATPase CHD5 has been linked to the progression of neuroblastoma tumors, yet the underlying mechanisms behind the tumor suppressor role of CHD5 are unknown. In this study, we purified the human CHD5 complex and found that CHD5 is a component of the full NuRD transcriptional repressor complex, which also contains methyl-CpG binding proteins and histone deacetylases. The CHD5/NuRD complex appears mutually exclusive with the related CHD4/NuRD complex as overexpression of CHD5 results in loss of the CHD4 protein in cells. Following a search for genes that are regulated by CHD5 in neuroblastoma cells, we found that CHD5 binds to and represses the G2/M checkpoint gene WEE1. Reintroduction of CHD5 into neuroblastoma cells represses WEE1 expression, demonstrating that CHD5 can function as a repressor in cells. A catalytically inactive mutant version of CHD5 is able to associate with a NuRD cofactor but fails to repress transcription. Our study shows that CHD5 is a ...
A challenge for functional genomics has been to make meaningful global measurements of the interactions between transcription factors (and cofactors) and DNA. It has been difficult, especially in large genomes, to explicitly map individual binding sites and individual factor-target gene interactions. Johnson et al. (see the Perspective by Fields) have developed a combination of chromatin immunoprecipitation and ultrahigh-throughput sequencing to achieve high specificity and 50-base pair resolution. This approach was used to study regulation by neuron-restrictive silencer factor (NRSF, also known as REST, for repressor element-1 silencing transcription factor) and identify targets of key positive regulators of pancreatic neuroendocrine development. D. S. Johnson, A. Mortazavi, R. M. Myers, B. Wold, Genome-wide mapping of in vivo protein-DNA interactions. Science 316, 1497-1502 (2007). [Abstract] [Full Text]. S. Fields, Site-seeing by sequencing. Science 316, 1441-1442 (2007). [Summary] [Full ...
Results We found that VEGF release from BMSCs was significantly increased in parallel with high level of HIF-1α in BMSCs following anoxia or hypoxia in time-dependent manner. Furthermore, the level of VEGF released from BMSCs overexpressing CREG and the expression of HIF-1α in BMSCs overexpressing CREG were higher than the normal BMSCs under hypoxia. Rather, HIF-1α steady-state mRNA was also affected by CREG. This effect was associated with constitutive activation of phosphatidylinositol 3-kinase (PI3K)/Akt and its effector p70 S6 kinase (p70S6K), but not extracellular-signal regulated kinase 1/2. The use of small molecule inhibitors LY294002 or rapamycin to inhibit PI3K/Akt and p70S6K activities, respectively, resulted in diminished HIF-1α activation and subsequent VEGF expression. RNA interference-mediated knockdown of HIF-1α suppressed CREG-induced VEGF synthesis and angiogenic tube formation, confirming that the effect was HIF-1α specific.. ...
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The Polycomb group (PcG) proteins are transcriptional repressors that regulate lineage choices during development and differentiation. Recent studies have advanced our understanding of how the PcG proteins regulate cell fate decisions and how their deregulation potentially contributes to cancer. In this Review we discuss the emerging roles of long non-coding RNAs (ncRNAs) and a subset of transcription factors, which we call cell fate transcription factors, in the regulation of PcG association with target genes. We also speculate about how their deregulation contributes to tumorigenesis. ...
In the present study, we demonstrate that ectopic expression of the corepressor protein Sin3 leads to stabilization of both transfected and endogenous p53. That this effect is a direct impact of interaction of Sin3 with p53 is supported by our finding that a 15-amino-acid deletion mutant of p53 that is incapable of interacting with Sin3 also fails to be stabilized by this protein. Similarly, mutation of proline 71 of p53 to arginine or leucine significantly impairs Sin3 binding and Sin3-mediated stabilization. These data support a tight correlation between Sin3 binding and stabilization of p53. Our data also indicate that stabilization by Sin3 is likely the result of inhibition of proteasome-mediated degradation of p53. Interestingly, unlike p14ARF, MDMX, and pRB, Sin3 does not require the presence of MDM2 for this effect. Therefore, these findings point to the existence of a potentially novel pathway for p53 stabilization that does not involve inhibition of MDM2 function.. At least two proteins ...
Squamous cell carcinoma (SCC) is a treatment-refractory subtype of human cancer arising from stratified epithelium of the skin, lung, esophagus, oropharynx and other tissues. A unifying feature of SCC is high-level expression of the p53 related protein p63 (TP63) in 80% of cases. The major p63 isoform expressed in SCC is ΔNp63α, an N-terminally truncated form which functions as a key SCC cell survival factor by mechanisms that are unclear. In this study we demonstrate that ΔNp63α associates with HDAC1 and HDAC2 to form an active transcriptional repressor complex that can be targeted to therapeutic advantage. Repression of pro-apoptotic Bcl-2 family member genes including PUMA by p63/HDAC is required for survival of SCC cells. Cisplatin chemotherapy, a mainstay of SCC treatment, promotes dissociation of p63 and HDAC from the PUMA promoter, leading to increased histone acetylation, PUMA activation and apoptosis. These effects are recapitulated upon targeting the p63/HDAC complex selectively ...
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The nonspecific DNA binding capacity of the lac repressor protein has been assessed by two different methods. Boundary sedimentation of repressor and calf thymus DNA fragmented by shearing yielded dissociation constants in good agreement with values previously reported in the literature. The b ...
Previously, this and other laboratories have identified two regions on YY1 that mediate transcriptional repression (1, 26-28). Herein, we have further delineated the repression domain in one region to amino acids 170-200. More important, we have identified a novel mammalian corepressor that interacts with this domain.. YY1, like most eukaryotic repressors described to date, seems to act directly on the general transcription machinery-a mechanism referred to as active repression (for review, see refs. 29-32). Three types of domains have been identified thus far in active transcriptional repressors (for review, see refs. 29-32): alanine-rich, glutamine-rich, and/or proline-rich. Currently, it is not known whether these repression domains function by contact with the general transcriptional machinery, and proteins that interact with these domains have yet to be identified. Inspection of the YY1 amino acid sequence has failed to reveal any resemblance to the primary sequence motifs that characterize ...
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5-CACGTG-3. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
Start is the main decision point in eukaryotic cell cycle in which cells commit to a new round of cell division. It involves the irreversible activation of a transcriptional program by G1 CDK-cyclin complexes through the inactivation of Start transcriptional repressors, Whi5 in yeast or Rb in mammals. Here we provide novel keys of how Whi7, a protein related at sequence level to Whi5, represses Start. Whi7 is an unstable protein, degraded by the SCFGrr1 ubiquitin-ligase, whose stability is cell cycle regulated by CDK1 phosphorylation. Importantly, Whi7 associates to G1/S gene promoters in late G1 acting as a repressor of SBF-dependent transcription. Our results demonstrate that Whi7 is a genuine paralog of Whi5. In fact, both proteins collaborate in Start repression bringing to light that yeast cells, as occurs in mammalian cells, rely on the combined action of multiple transcriptional repressors to block Start transition.. ...
MicroRNA (miR)-155 is upregulated in breast cancer cells and in sera of patients with breast cancer, but its clinical relevance remains uncertain. The objective of the present effort was to address the transcriptional regulation of miR-155. A bioinformatics analysis of public datasets validated upre …
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Repressors and activators are often allosteric proteins whose function is modified by ligand binding. In general, a ligand alters the conformation of the protein and affects its ability to bind to specific DNA sequences. For example, some repressors control the synthesis of enzymes for a catabolic pathway. In the absence of substrate for these enzymes, the genes are repressed. When substrate is present, it binds to the repressor, causing the repressor to dissociate from the DNA and allowing the genes to be transcribed. Ligands that bind to and inactivate repressors are called inducers because they induce transcription of the genes controlled by the repressors. In contrast, some repressors that control the synthesis of enzymes for a biosynthetic pathway bind to DNA only when associated with a ligand. The ligand is often the end product of the biosynthetic pathway. This regulatory mechanism ensures that the genes are turned off as product accumulates. Ligands that bind to and activate repressors ...
The galactose represser protein from E. coli has a pI of about 5.9. While purification protocols were being designed, it was found to bind to a Mono-S column at pH values of 7 and below. (Mono-S columns have S-type sulfonic acid.
In chapter 3, The Sense of Sensibility, author Wendy Jones uses scenes from one of Jane Austens most celebrated novels to illustrate the functioning of the bodys stress response system.. 0 Comments. ...
1IGQ: An Src homology 3-like domain is responsible for dimerization of the repressor protein KorB encoded by the promiscuous IncP plasmid RP4.
Deposition date: 2010-06-21 Original release date: 2010-07-09. Authors: Goel, Anupam. Citation: Goel, Anupam; Tripet, Brian; Tyler, Robert; Nebert, Lucas; Copie, Valerie. Backbone Amide Dynamics Studies of Apo-L75F-TrpR, a Temperature-Sensitive Mutant of the Tryptophan Repressor Protein (TrpR): Comparison with the (15)N NMR Relaxation Profiles of Wild-Type and A77V Mutant Apo-TrpR Repressors. Biochemistry 49, 8006-8019 (2010).. Assembly members: ...
Exhibits NF-kappaB binding activity. Involved in ameboidal-type cell migration; dorsal/ventral pattern formation; and epithelial to mesenchymal transition. Localizes to nucleus. Is expressed in several structures, including blastodisc; germ ring; mesoderm; musculature system; and pharyngeal arch. Orthologous to human SNAI1 (snail family transcriptional repressor 1 ...
General Repressor Of Transcription; Forms Complex With Cyc8p, Involved In The Establishment Of Repressive Chromatin Structure Through Interactions With Histones H3 And H4, Appears To Enhance Expression Of Some Genes
Although genetic evidence suggests that SNI1 is a transcriptional repressor (Li et al., 1999), its vanishingly low level of expression and lack of sequence homology with known proteins or domains have made the application of conventional biochemical and molecular methodologies ineffective. To remedy this, we developed a combination of genetic and genomic approaches to dissect the structure and function of this novel regulator.. The use of the GFP-SNI1 fusion protein allowed the observation of the subcellular localization of SNI1 in planta. Because of the extremely low protein levels and the background fluorescence of chlorophyll in green tissues, GFP-SNI1 fluorescence was visible only in roots. Although SAR is expressed only in the aerial parts of the plant, SNI1 is likely to be functional in roots because in the sni1 mutant, root development is substantially impaired. The GFP-SNI1 fusion complemented all of the sni1 phenotypes in both shoot and root tissues, indicating that the fusion protein ...
Complete information for MXD4 gene (Protein Coding), MAX Dimerization Protein 4, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The COP1/SPA E3 ubiquitin ligase is a general repressor of photomorphogenesis in the dark. The four SPA family members, SPA1-4, have been shown to have partially overlapping but distinct functions in response to light. SPA1 is a key player of repressing photomorphogenesis, while SPA2 only functions in the dark. SPA2, on one hand, is not as stable as SPA1 in the light. On the other hand, the COP1/SPA2 complex also loses its biological function in the light via unknown mechanisms. This functional divergence of SPA1 and SPA2 has been shown to depend on the differences between their protein sequences. By phenotypical studies of the transgenic seedlings expressing chimeric constructs, I will try to answer the question that which domain is responsible for the diverged function of SPA1 and SPA2. I will also attempt to reveal the molecular basis underlying this divergence. ...
Inducer does NOT bind DNA. Inducer binds either activator OR repressor. It makes activator BETTER able to bind DNA = more transcription OR it makes repressor LESS able to bind DNA = transcription ...
Prohibitin, an evolutionarily conserved gene situated on chromosome 17q21, was originally identified as a gene with antiproliferative properties. Studies of a Japanese population have shown prohibitin to be somatically mutated in a proportion of breast tumours. The gene has not heretofore been shown to have an association with inherited forms of breast or other cancers. In this thesis the technique of denaturing gradient gel electrophoresis (DGGE) was developed to analyse the complete coding sequence of the prohibitin gene from fragments generated by the polymerase chain reaction (PCR). This was achieved by examining the melting profiles of different regions of the prohibitin sequence with the melt map program MELT87. Four overlapping fragments were designed and subsequently amplified by reverse transcription PCR thus enabling analysis of the prohibitin cDNA sequence by DGGE. A further five fragments were developed for analysis of prohibitin from genomic DNA. These five fragments were generated ...
Complete information for ASXL3 gene (Protein Coding), Additional Sex Combs Like 3, Transcriptional Regulator, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Filtering by: Creator Maria A. Schumacher Remove constraint Creator: Maria A. Schumacher Degree Ph.D. Remove constraint Degree: Ph.D. Department Dept. of Biochemistry and Molecular Biology Remove constraint Department: Dept. of Biochemistry and Molecular Biology Keyword crystallography Remove constraint Keyword: crystallography Keyword repressor proteins Remove constraint Keyword: repressor proteins Keyword dna-binding proteins Remove constraint Keyword: dna-binding proteins Collection Scholars Archive Remove constraint Collection: Scholars Archive ...
cdna:known chromosome:VEGA66:2:153345845:153404007:1 gene:OTTMUSG00000019852 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Asxl1 description:additional sex combs like 1 (Drosophila ...
The inducer binds to the repressor protein and induces a conformational change so that the repressor does not bind to the operator DNA sequence ...
GF ID Tup_N #=GF AC PF08581.11 #=GF DE Tup N-terminal #=GF AU Wood V;0000-0001-6330-7526 #=GF AU Finn RD;0000-0001-8626-2148 #=GF SE Pfam-B_9595 (release 19.0) #=GF GA 28.70 28.70; #=GF TC 28.70 28.70; #=GF NC 28.60 28.50; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq #=GF TP Domain #=GF RN [1] #=GF RM 12234489 #=GF RT Mutations of the WD repeats that compromise Tup1 repression #=GF RT function maintain structural integrity of the WD domain #=GF RT trypsin-resistant core. #=GF RA Zhang Z, Varanasi U, Carrico P, Trumbly RJ; #=GF RL Arch Biochem Biophys. 2002;406:47-54. #=GF DR INTERPRO; IPR013890; #=GF DR SO; 0000417; polypeptide_domain; #=GF CC The N-terminal domain of the Tup protein has been shown to #=GF CC interact with the Ssn6 transcriptional co-repressor [1]. #=GF SQ 757 #=GS A0A1B8CSS6_9PEZI/19-73 AC A0A1B8CSS6.1 #=GS A0A1S8A7M6_ROSNE/15-84 AC A0A1S8A7M6.1 #=GS A0A0W0FM89_9AGAR/18-90 AC A0A0W0FM89.1 #=GS S3D7V2_GLAL2/16-85 AC S3D7V2.1 #=GS ...
The repressor CytR and the activator CRP, two dimeric proteins, interact to form a complex repressor nucleoprotein in the intergenic region. When only CRP is bound to this promoter, it functions as an activator, and then, when CytR binds to DNA and to CRP, the activation is repressed because CytR masks an activating region of CRP that otherwise would contact the RNA polymerase to activate transcription ,CITS:[ 8736525][10766824],. The CytR protein cannot act alone; the synergistic DNA binding is increased by direct interaction with CRP ,CITS:[1962841][ 2170326][1649947],. At times CytR also repositions CRP to alternative DNA-binding sites that are not functional for activation ,CITS:[ 8736525 ...
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Plasmid pFUW-tetO-GFI1B from Dr. Filipe Pereiras lab contains the insert Growth Factor Independent 1B transcriptional repressor and is published in Cell Rep. 2018 Dec 4;25(10):2821-2835.e7. doi: 10.1016/j.celrep.2018.11.032. This plasmid is available through Addgene.
Plasmid pAKgfplux2 from Dr. Attila Karsis lab contains the insert Bacterial luciferase-lacIq repressor gene and is published in Plasmid. 2007 Jan 4. ():. This plasmid is available through Addgene.
Signal transductionRegulatory functionsDNA interactionsGTP-sensing transcriptional pleiotropic repressor CodY (TIGR02787; HMM-score: 351.8) ...
PRDM1/Blimp1山羊多克隆抗体(ab106766)可与人样本反应并经WB, IHC, ChIP实验严格验证,被1篇文献引用。所有产品均提供质保服务,中国75%以上现货。
PRDM1/Blimp1兔多克隆抗体(ab119401)可与小鼠, 大鼠, 人样本反应并经WB实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
How is B Lymphocyte-Induced Maturation Protein abbreviated? BLIMP stands for B Lymphocyte-Induced Maturation Protein. BLIMP is defined as B Lymphocyte-Induced Maturation Protein somewhat frequently.
TY - JOUR. T1 - SUMOylation of Blimp-1 is critical for plasma cell differentiation. AU - Ying, Hsia Yuan. AU - Su, Shin Tang. AU - Hsu, Pang Hung. AU - Chang, Che Chang. AU - Lin, I. Ying. AU - Tseng, Yu Hsuan. AU - Tsai, Ming Daw. AU - Shih, Hsiu Ming. AU - Lin, Kuo I.. PY - 2012/7. Y1 - 2012/7. N2 - Transcriptional repressor B lymphocyte-induced maturation protein-1 (Blimp-1) is a master regulator of plasma cell differentiation. Here we show that Blimp-1 is covalently modified by SUMO1 at lysine 816, a modification mediated by SUMO E3 ligase PIAS1. Mutation of Blimp-1 lysine 816 reduces transcriptional repression-correlating with a reduced interaction with a histone deacetylase, HDAC2-and impairs differentiation of antibody-secreting cells. Thus, the SUMO pathway critically regulates Blimp-1 function during plasma cell differentiation.. AB - Transcriptional repressor B lymphocyte-induced maturation protein-1 (Blimp-1) is a master regulator of plasma cell differentiation. Here we show that ...
Polycomb group (PcG) protein are transcriptional repressors that regulate many crucial developmental and physiological procedures in the cell. initiation, advancement, and development. Finally, we discuss the potential worth of PcG protein as molecular biomarkers for the treatment and medical diagnosis of tumor, and as molecular goals for tumor therapy. to human beings.5,6 PcG meats possess been proven to control different biological functions during embryonic advancement, such as cell family tree and fate decisions, cellular memory, come cell function, and tissues homeostasis.7-13 PcG targets include different genes encoding transcription factors, receptors, signaling proteins, morphogens, and regulators included in all main developing pathways.8 During embryonic advancement, the PcG protein and other epigenetic government bodies participate in rules of the transcriptional system, in which the primordial pluripotent embryonic originate cells show temporally limited transcriptional service and ...
摘要(Abstract): 目的探讨不明原因复发性流产(URSA)患者绒毛中滤泡辅助性T(follicular helper T,Tfh)细胞相关因子白介素-21(interleukin-21,IL-21)、趋化因子受体-5(CXC chemokine receptor-5,CXCR5)、B细胞淋巴瘤分子6(B cell lymphoma 6,Bcl-6)和B淋巴细胞诱导成熟蛋白1(B lymphocyte-induced maturation protein 1,Blimp-1)的表达部位和表达水平及其与URSA发病的免疫学机制。方法收集30例URSA患者(URSA组)和30例要求人工流产的正常早孕妇女(对照组)绒毛组织,采用免疫组织化学法检测IL-21、CXCR5、Bcl-6和Blimp-1的表达情况,采用Pearson相关系数分析4种因子之间的相关性。结果 URSA组绒毛组织中IL-21、CXCR5、Bcl-6和Blimp-1表达水平明显高于对照组( ...
The Proline-Rich Homeodomain protein (PRH/Hex) is a transcription factor that functions as an important regulator of vertebrate development and many other processes in the adult including haematopoiesis. The Groucho/TLE family of co-repressor proteins also regulate development and modulate the activity of many DNA-binding transcription factors during a range of diverse cellular processes including haematopoiesis. We have shown previously that PRH is a repressor of transcription in haematopoietic cells and that an Eh-1 motif present within the N-terminal transcription repression domain of PRH mediates binding to Groucho/TLE proteins and enables co-repression. Here we demonstrate that PRH regulates the nuclear retention of TLE proteins during cellular fractionation. We show that transcriptional repression and the nuclear retention of TLE proteins requires PRH to bind to both TLE and DNA. In addition, we characterise a trans-dominant negative PRH protein that inhibits wild type PRH activity by ...
Upon EBV infection, mature human B cells become activated, grow and proliferate. In vivo, in the presence of T cells or T cell-derived factors, infected cells can enter the germinal centre and differentiate into memory B cells, the site of long-term EBV latency and persistence. However, it has not been established what happens if T cell help is unavailable (Th-ve). Usually in the absence of T cell help, antigen-activated B cells can enter the default plasma cell differentiation pathway, resulting in antibody-producing plasma cells. We suggest EBV has evolved to prevent default plasma cell differentiation, thus favouring latency in memory B cells, through specific repression of the plasma cell differentiation factors p18INK4c and B lymphocyte-induced maturation protein-1 (BLIMP-1), by the viral transcription factors EBNA3A and EBNA3C that act in vitro to support the activated B-blast population in establishing continuously proliferating lymphoblastoid cell lines (LCLs). Since the repression of ...
TY - JOUR. T1 - Components of the SMRT corepressor complex exhibit distinctive interactions with the POZ domain oncoproteins PLZF, PLZF-RAR∅, and BCL-6. AU - Wong, Chi Wai. AU - Privalsky, Martin L.. PY - 1998/10/16. Y1 - 1998/10/16. N2 - Many transcription factors function by repressing gene transcription. For a variety of these transcription factors the ability to physically recruit auxiliary proteins, denoted corepressors, is crucial for the ability to silence gene expression. We and others have previously implicated the SMRT corepressor in the actions of the PLZF transcription factor and in the function of its oncogenic derivative, PLZF-retinoic acid receptor (RARα), in promyelocytic leukemia. We report here that PLZF, and a structurally similar transcriptional repressor, BCL-6, can interact with a variety of corepressor proteins in addition to SMRT, including the mSin3A protein and (for PLZF) histone deacetylase-1. Unexpectedly, these additional interactions with corepressor components ...
TY - JOUR. T1 - Mutations in λ repressors amino-terminal domain. T2 - Implications for protein stability and DNA binding. AU - Hecht, M. H.. AU - Nelson, H. C.M.. AU - Sauer, R. T.. N1 - Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 1983. Y1 - 1983. N2 - The DNA binding properties of 52 different single-amino acid substitutions in λ repressors amino-terminal domain have been characterized. Seven proteins bearing mutations that change solvent-exposed side chains have been purified. The amino-terminal domains of these mutant repressors are folded and are comparable to the wild-type amino-terminal domain in thermal stability. In contrast, a purified mutant repressor bearing a substitution in a buried side chain contains an amino-terminal domain with decreased thermal stability. We argue that mutations that alter solvent-exposed wild-type side chains define residues that form the operator DNA binding surface of λ repressor whereas completely or partially buried mutations ...
Project Summary/Abstract Even though Inducible cAMP Early Repressor (ICER) has the functional characteristics of a tumorsuppressor, there is no genetic evidence to demonstrate that ICER is a bona fide tumor suppressor geneproduct. Thus, altered post-translational events might be the cause of the observed abnormalities of ICERprotein expression in cancer cells. On this basis it is hypothesized that in cancer cells, ICER isderegulated by ubiquitination resulting in constitutive proteasomal degradation and/or abnormalsubcellular localization. Finding alternatives to rescue endogenous ICER nuclear expression in malignantcells could lead to the development of novel cancer treatment modalities. Through this project, we will studythe mechanisms and physiological consequences of ICER ubiquitination and subcellular localization. We willuse melanoma as a paradigm for the study. This study will focus on two specific aims.Aim 1. Determine the functional and physiological consequences of ICER ubiquitination ...
Clone REA516 recognizes the human and mouse interferon regulatory factor 8 (IRF-8) antigen, a 50 kDa transcription factor, which is also known as interferon consensus sequence-binding protein (ICSBP). IRF-8 belongs to the family of interferon regulatory transcription factors, which consists of nine members in both human and mice and is characterized by a conserved N-terminal DNA-binding domain with a unique tryptophan pentad repeat. IRF-8 is expressed at very high levels in mononuclear phagocytes, and regulates granulocyte/macrophage differentiation and dendritic cell (DC) development. Acting in heterodimeric complexes with other transcription factors, IRF-8 also controls the transcriptional response of mature myeloid cells to interferons and Toll-like receptor agonists, a response in which IRF-8 binds and transactivates the promotors of IL12B and NOS2. IRF-8 knockout mice display a loss of CD8a+ lymphoid DCs, CD103+ tissue myeloid DCs, and plasmacytoid DCs.Additional information: Clone REA516 displays
View Notes - Handout16Activation from BIS 101 at UC Davis. Model 2: TRANSCRIPTIONAL REPRESSOR - repressor active mRNA____________ > + repressor X inactive repression of repressor active
Rabbit anti IRF8 antibody recognizes Interferon regulatory factor 8, (IRF8) also known as Interferon consensus sequence-binding protein (I
It has been reported that at the onset of pupariation there is an increase of lipid droplets in PG cells that can be seen by Oil Red O staining of precisely staged 120 h AEL wandering larvae (Talamillo et al., 2013). At 120 h AEL, phm,CTCFRNAi PGs had a reduced content of lipid droplets in comparison to controls (Fig. 4B, compare middle and left panels), likely due to developmental delay. However, there is also an increase of lipid droplets at the end of larval development in phm,CTCFRNAi PG cells which tend to be slightly higher than in control animals (Fig. 4B, compare right and left panels and supplementary material Fig. S4). Since the subcellular lipid accumulation phenotype of phm,CTCFRNAi PG cells is similar to that of Niemann-Pick type C (npc) mutants (Huang et al., 2005), we analyzed dnpc1a transcriptional levels but we found no changes between control and phm,CTCFRNAi larvae (data not shown). Increased lipid accumulation in the fat body in EcR knockdown larvae has been reported ...
EN] Mot3 and Rox1 are transcriptional repressors of hypoxic genes. Both factors recently have been found to be involved in the adaptive response to hyperosmotic stress, with an important function in the adjustment of ergosterol biosynthesis. Here, we determine the gene expression profile of a mot3 rox1 double mutant under acute osmostress at the genomic scale in order to identify the target genes affected by both transcription factors upon stress. Unexpectedly, we find a specific subgroup of osmostress-inducible genes to be under positive control of Mot3. These Mot3-activated stress genes also depend on the general stress activators Msn2 and Msn4. We confirm that both Mot3 and Msn4 bind directly to some promoter regions of this gene group. Further-more, osmostress-induced binding of the Msn2 and Msn4 factors to these target promoters is severely affected by the loss of Mot3 function. The genes repressed by Mot3 and Rox1 preferentially encode proteins of the cell wall and plasma membrane. Cell ...
Transcription of genes encoding enzymes for the biosynthesis of methionine and trytophan in Escherichia coli is regulated by the ligand-activated met and trp repressors. X-ray crystallographic studies show how these two small proteins, although similar in size and function, have totally different three-dimensional structures and specifically recognize their respective DNA operator sequences in different ways. A common feature is that both repressors bind as cooperative arrays to tandem repeats of 8 base-pair Met or Trp boxes respectively, and the consensus sequences share the rare tetranucleotide CTAG. A series of structural and functional studies have shown how the two repressors discriminate between their operators, using a combination of direct contacts between side chains and bases, and indirect sensing of conformational properties of the DNA. ...
Specific wiring of gene-regulatory networks is likely to underlie much of the phenotypic difference between species, but the extent of lineage-specific regulatory architecture remains poorly understood. The essential vertebrate transcriptional repressor REST (RE1-Silencing Transcription Factor) targets many neural genes during development of the preimplantation embryo and the central nervous system, through its cognate DNA motif, the RE1 (Repressor Element 1). Here we present a comparative genomic analysis of REST recruitment in multiple species by integrating both sequence and experimental data. We use an accurate, experimentally validated Position-Specific Scoring Matrix method to identify REST binding sites in multiply aligned vertebrate genomes, allowing us to infer the evolutionary origin of each of 1,298 human RE1 elements. We validate these findings using experimental data of REST binding across the whole genomes of human and mouse. We show that one-third of human RE1s are unique to ...
Specific wiring of gene-regulatory networks is likely to underlie much of the phenotypic difference between species, but the extent of lineage-specific regulatory architecture remains poorly understood. The essential vertebrate transcriptional repressor REST (RE1-Silencing Transcription Factor) targets many neural genes during development of the preimplantation embryo and the central nervous system, through its cognate DNA motif, the RE1 (Repressor Element 1). Here we present a comparative genomic analysis of REST recruitment in multiple species by integrating both sequence and experimental data. We use an accurate, experimentally validated Position-Specific Scoring Matrix method to identify REST binding sites in multiply aligned vertebrate genomes, allowing us to infer the evolutionary origin of each of 1,298 human RE1 elements. We validate these findings using experimental data of REST binding across the whole genomes of human and mouse. We show that one-third of human RE1s are unique to ...
The majority of bacterial gene regulators bind as symmetric dimers to palindromic DNA operators of 12-20 base pairs (bp). Multimeric forms of proteins, including tetramers, are able to recognize longer operator sequences in a cooperative manner, although how this is achieved is not well understood due to the lack of complete structural information. Models, instead of structures, of complete tetrameric assembly on DNA exist in literature. Here we present the crystal structures of the multidrug-binding protein TtgV, a gene repressor that controls efflux pumps, alone and in complex with a 42-bp DNA operator containing two TtgV recognition sites at 2.9 Å and 3.4 Å resolution. These structures represent the first full-length functional tetrameric protein in complex with its intact DNA operator containing two continuous recognition sites. TtgV binds to its DNA operator as a highly asymmetric tetramer and induces considerable distortions in the DNA, resulting in a 60° bend. Upon binding to its ...
TY - JOUR. T1 - Mechanism of corepressor binding and release from nuclear hormone receptors. AU - Nagy, Laszlo. AU - Kao, Hung Ying. AU - Love, James D.. AU - Li, Chuan. AU - Banayo, Ester. AU - Gooch, John T.. AU - Krishna, V.. AU - Chatterjee, K.. AU - Evans, Ronald M.. AU - Schwabe, John W.R.. PY - 1999/12/15. Y1 - 1999/12/15. N2 - The association of transcription corepressors SMRT and N-CoR with retinoid and thyroid receptors results in suppression of basal transcriptional activity. A key event in nuclear receptor signaling is the hormone-dependent release of corepressor and the recruitment of coactivator. Biochemical and structural studies have identified a universal motif in coactivator proteins that mediates association with receptor LBDs. We report here the identity of complementary acting signature motifs in SMRT and N- CoR that are sufficient for receptor binding and ligand-induced release. Interestingly, the motif contains a hydrophobic core (ΦxxΦΦ) similar to that found in NR ...
Recent studies in mammalian systems, where methylation clearly plays a role in gene silencing, indicate that methylation mediates the formation of a multiprotein repression complex that induces changes in histone acetylation. This complex is based on the methyl‐binding protein MeCP2 which has been shown to contain, in addition to its methyl binding domain (MBD), a transcriptional repressor domain (TRD) (Nan et al., 1997). This TRD has been shown recently to overlap with a region that interacts directly with the corepressor mSin3A. Immunoprecipitation experiments show that antibodies raised against MeCP2 coprecipitate MeCP2, mSin3A, HDAC1, and HDAC2 (Nan et al., 1998).. Experiments using the deacetylase inhibitor trichostatin A relieved the TRD‐mediated repression induced by MeCP2 (Nan et al., 1998). Although these important experiments revealed the link between MeCP2‐induced repression and histone deacetylase activity, they failed to show directly that methyl groups present at the 5′ end ...
The Groucho (Gro)/TLE/Grg Family Of Corepressors Operates In Many Signaling Pathways (including Notch And Wnt). Gro/TLE Proteins Recognize A Wide Range Of Transcriptional Repressors By Binding To Divergent Short Peptide Sequences, Including A C-terminal
mitochondrion, nucleus, transcriptional repressor complex, DNA binding, E-box binding, histone deacetylase binding, protein homodimerization activity, RNA polymerase II proximal promoter sequence-specific DNA binding, sequence-specific DNA binding, transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
The Lazar laboratory is studying the transcriptional regulation of metabolism. We are particularly focused on the role played by nuclear receptors (NRs). In the absence of ligand, NRs bind to DNA and function as potent transcriptional repressors by recruiting corepressor complexes that include the chromatin modulating enzyme histone deacetylase 3 (HDAC3). We are studying the tissue-specific and physiological roles of the corepressor complexes using by combining genomic, genetic, proteomic, bioinformatic, and metabolic phenotyping approaches. We are especially interested in the circadian NR Rev-erb alpha, which utilizes the corepressor complex to potently repress transcription. Rev-erb alpha is a key repressive component of the circadian clock that coordinates metabolism and biological rhythms. We are also studying PPAR gamma, a nuclear receptor that is a master regulator of adipocyte (fat cell) differentiation. Ligands for PPAR gamma have potent antidiabetic activity, and thus PPAR gamma ...
Strains, cell growth and transformations: The S. cerevisiae strain RZ53-6Δtup1 (MATα trp1-289 leu2-3, 112 ura3-52 ade1-100 tup1::ura3) was described previously (Zhanget al. 1991). The following S. cerevisiae strains were constructed by standard methods of yeast genetics (Roseet al. 1990): MZ14-29 (MATa/MATα trp1/trp1 leu2/leu2 ura3/ura3 lys2/lys2 tif51A::TRP1/tif51A::TRP1 tup1::URA3/tup1::URA3 ura3::AZ4/ura3::AZ4 gal-/gal-) and MZ12-16 (MATa trp1 leu2 lys2 his3 tup1::URA3 ura3::AZ4 gal-).. Yeast cells were transformed as described previously (Chenet al. 1992).. Escherichia coli HB101 was maintained and transformed as described previously (Ausubelet al. 1994).. Enzymes and general methods for plasmid constructions: Plasmid constructions were carried out according to standard protocols (Ausubelet al. 1994). Enzymatic reactions were carried out under the conditions recommended by the vendors. Most restriction enzymes and T4 DNA ligase were purchased from New England Biolabs (Beverly, MA) or ...
Predicted to contribute to histone deacetylase activity. Predicted to be involved in histone deacetylation; negative regulation of transcription by RNA polymerase II; and positive regulation of chromatin silencing. Predicted to localize to the nucleoplasm and transcriptional repressor complex. Orthologous to human MIER1 (MIER1 transcriptional regulator ...
Interactions between transcription factors, bound to separate operator sites, commonly play an important role in gene regulation by mediating cooperative binding to the DNA. However, few detailed structural models for understanding the molecular basis of such cooperativity are available. The cI repressor of bacteriophage l is a classic example of a protein that binds to its operator sites cooperatively. The C-terminal domain of the repressor mediates dimerization, as well as a dimer-dimer interaction that results in the cooperative binding of two repressor dimers to adjacent operator sites. We have determined the structure of the l repressor C-terminal domain and identified the interactions that mediate cooperativity. Using the structure, genetics and biochemical data, we have determined the cooperative binding of two l repressor dimers at adjacent operator sites ...
The modulation of the affinity of DNA-binding proteins by small molecule effectors for cognate DNA sites is common to both prokaryotes and eukaryotes. However, the mechanisms by which effector binding to one domain affects DNA binding by a distal domain are poorly understood structurally. In initial studies to provide insight into the mechanism of effector-modulated DNA binding of the lactose repressor family, we determined the crystal structure of the purine repressor bound to a corepressor and purF operator. To extend our understanding, we have determined the structure of the corepressor-free corepressor-binding domain of the purine repressor at 2.2 A resolution. In the unliganded state, structural changes in the corepressor-binding pocket cause each subunit to rotate open by as much as 23 degrees, the consequences of which are the disengagement of the minor groove-binding hinge helices and repressor-DNA dissociation. Mechanism of corepressor-mediated specific DNA binding by the purine ...
We provide here several lines of evidence for the identification of Nrg1 as a transcriptional repressor responsible for glucose repression of the STA1 gene. First, nrg1Δcells, when grown under the repressed conditions, exhibit dramatically increased glucoamylase activity which is comparable to that of cells grown under the derepressed conditions. Second, Northern analyses show that the increased glucoamylase level is correlated with the increased level of STA1 transcript in nrg1Δ cells. Third, gel retardation and DNase I footprinting experiments demonstrate that Nrg1 binds specifically to UAS1-1 element of the STA1promoter. Fourth, tethering of Nrg1 to DNA via LexA-Nrg1 represses transcription of a target gene in glucose-grown cells, and the repression requires the Ssn6-Tup1 complex, which is needed for repression of diverse genes involved in many different cellular processes. And finally, two-hybrid and GST pull-down experiments demonstrate the physical interaction between Nrg1 and Ssn6 both ...
Anxiety disorders and depression are well-documented in subjects exposed to adverse childhood events. Recently, maternal obesity and/or maternal consumption of high-fat diets (HFD) have been also proposed as risk factors for offspring mental health. Here using an animal model in rats, we explored the combinatorial effects of a maternal HFD (40% of energy from fat without impact on maternal weight; during gestation and lactation) and maternal separation (MS) in offspring. In the prefrontal cortex (PFC) of pups, MS led to changes in the expression of several genes such as Bdnf (brain derived neurotrophic factor), 5HT-r1a (serotonin receptor 1a) and Rest4 (neuron-restrictive silencer element, repressor element 1, silencing transcription factor (Rest), splicing variant 4 ...
EntrezGene ,Full_name_from_nomenclature_authority=CCCTC-binding factor (zinc finger protein) ,GeneID=10664 ,LocusTag=- ,Modification_date=20120108 ,Nomenclature_status=O ,Other_designations=11 zinc finger transcriptional repressor;;11-zinc finger protein;;CTCFL paralog;;transcriptional repressor CTCF ,Symbol=CTCF ,Symbol_from_nomenclature_authority=CTCF ,Synonyms=- ,chromosome=16 ,dbXrefs=HGNC:13723;;MIM:604167;;Ensembl:ENSG00000102974;;HPRD:05005;;Vega:OTTHUMG00000137539;;EpiFactors:10664:genes ,description=CCCTC-binding factor (zinc finger protein) ,map_location=16q21-q22.3 ,tax_id=9606 ,tf?=yes ,transcription_factor= ,type_of_gene=protein-coding ...
TY - JOUR. T1 - CoREST. T2 - A functional corepressor required for regulation of neural- specific gene expression. AU - Andres, M. E.. AU - Burger, C.. AU - Peral-Rubio, M. J.. AU - Battaglioli, E.. AU - Anderson, M. E.. AU - Grimes, J.. AU - Dallman, J.. AU - Ballas, N.. AU - Mandel, Gail. PY - 1999/8/17. Y1 - 1999/8/17. N2 - Several genes encoding proteins critical to the neuronal phenotype, such as the brain type II sodium channel gene, are expressed to high levels only in neurons. This cell specificity is due, in part, to long-term repression in nonneural cells mediated by the repressor protein REST/NRSF (RE1 silencing transcription factor/neural-restrictive silencing factor). We show here that CoREST, a newly identified human protein, functions as a corepressor for REST. A single zinc finger motif in REST is required for CoREST interaction. Mutations of the motif that disrupt binding also abrogate repression. When fused to a Gal4 DNA-binding domain, CoREST functions as a repressor. CoREST ...
The human body consists of a multitude of cells of varying appearance and function. With a few exceptions they are genetically identical, and the key to their divergence lies in their different specific patterns of gene expression. Gene expression may be regulated at the level of transcription, in two opposing directions; either activation or repression. Gene transcription is controlled by transcription factors, which bind to regulatory DNA sequences, and direct gene expression in concert with auxiliary proteins. Among these the nuclear receptor corepressor N-CoR holds a central position. It serves as a docking unit between many different DNA-bound transcription factors, such as nuclear receptors, and large complexes of repressor proteins. Many repressor complexes of distinct compositions have been shown to contain N-CoR.. N-CoR plays a vital part in normal fetal development, and its involvement has been implicated in several pathological conditions. It has been shown to interact with unliganded ...
The Krueppel-associated box (KRAB) is a domain of around 75 amino acids that is found in the N-terminal part of about one third of eukaryotic Krueppel-type C2H2 zinc finger proteins (ZFPs) [ (PUBMED:14519192) ]. It is enriched in charged amino acids and can be divided into subregions A and B, which are predicted to fold into two amphipathic alpha-helices. The KRAB A and B boxes can be separated by variable spacer segments and many KRAB proteins contain only the A box [ (PUBMED:2023909) ]. The functions currently known for members of the KRAB-containing protein family include transcriptional repression of RNA polymerase I, II and III promoters, binding and splicing of RNA, and control of nucleolus function. The KRAB domain functions as a transcriptional repressor when tethered to the template DNA by a DNA-binding domain. A sequence of 45 amino acids in the KRAB A subdomain has been shown to be necessary and sufficient for transcriptional repression. The B box does not repress by itself but does ...
The Krueppel-associated box (KRAB) is a domain of around 75 amino acids that is found in the N-terminal part of about one third of eukaryotic Krueppel-type C2H2 zinc finger proteins (ZFPs) [ (PUBMED:14519192) ]. It is enriched in charged amino acids and can be divided into subregions A and B, which are predicted to fold into two amphipathic alpha-helices. The KRAB A and B boxes can be separated by variable spacer segments and many KRAB proteins contain only the A box [ (PUBMED:2023909) ]. The functions currently known for members of the KRAB-containing protein family include transcriptional repression of RNA polymerase I, II and III promoters, binding and splicing of RNA, and control of nucleolus function. The KRAB domain functions as a transcriptional repressor when tethered to the template DNA by a DNA-binding domain. A sequence of 45 amino acids in the KRAB A subdomain has been shown to be necessary and sufficient for transcriptional repression. The B box does not repress by itself but does ...
I am expressing a repressor protein that is toxic to cells if it is overexpressed. The theory is that, since it is a DNA binding protein, when the repressor is at high levels it binds to all of the DNA in the cell, wrecking havoc on the critter. I was looking at growth of critters containing the plasmid-borne repressor protein under the control of a pTrc promoter on LB plates containing different amounts of IPTG. I examined this in lacY+ and lacY- cells. In general, the cells did not grow much, if at all, in lacY+ cells. However, growth in lacY- cells was dependent on the amount of IPTG on the plate; too much IPTG and the critters died. Expression of a protein under the control of the repressor protein was also dependent on the amount of IPTG I had on the plate in lacY- cells. I couldnt assess this information for the lacY+ cells, because any cell that expressed the repressor expressed too much of it and killed the cell. Thus, I appear to have titratable control of the pTrc promoter in lacY- ...
TY - JOUR. T1 - Chromatin adaptor Brd4 modulates E2 transcription activity and protein stability. AU - Lee, A. Young. AU - Chiang, Cheng Ming. PY - 2009/1/30. Y1 - 2009/1/30. N2 - Brd4 is a chromatin adaptor containing tandem bromodomains binding to acetylated histone H3 and H4. Although Brd4 has been implicated in the transcriptional control of papillomavirus-encoded E2 protein, it is unclear how Brd4 regulates E2 function and whether the involvement of Brd4 in transactivation and transrepression is common to different types of E2 proteins. Using DNase I footprinting performed with in vitro reconstituted human papillomavirus (HPV) chromatin and nucleosome-free DNA templates, we found that Brd4 facilitates E2 binding to its cognate sequences in chromatin depending on bromodomains and the E2-interacting region of Brd4. Moreover, the coactivator and corepressor function of Brd4 requires at least one intact bromodomain and is mediated by its direct association with E2 proteins encoded by ...
TY - JOUR. T1 - CLONING IN ESCHERICHIA-COLI OF A BACILLUS-SUBTILIS ARGININE REPRESSOR GENE THROUGH ITS ABILITY TO CONFER STRUCTURAL STABILITY ON A FRAGMENT CARRYING GENES OF ARGININE-BIOSYNTHESIS. AU - Smith, Margaret Caroline MacHin. AU - MOUNTAIN, A AU - BAUMBERG, S PY - 1986. Y1 - 1986. M3 - Article. VL - 205. SP - 176. EP - 182. JO - Molecular Neurobiology. JF - Molecular Neurobiology. SN - 0893-7648. IS - 1. ER - ...
mouse interferon repressor protein: IRP is localized in cell sap and in ribosomal fraction of mouse cells; initially identified in chick embryo fibroblasts in state of hyperreactivity to repeated induction of poly(ri)poly(rc)
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Nakamichi, Norihito et al PSEUDO-RESPONSE REGULATORS 9, 7, and 5 Are Transcriptional Repressors in the Arabidopsis Circadian Clock. The Plant Cell 22.3 (2010): 594-605. Web. 27 Feb. 2020. ...
BACKGROUND: The CCTC-binding factor (CTCF) protein is involved in genome organization, including mediating three-dimensional chromatin interactions. Human patient lymphocytes with mutations in a single copy of the CTCF gene have reduced expression of enhancer-associated genes involved in response to stimuli. We hypothesize that CTCF interactions stabilize enhancer-promoter chromatin interaction domains, facilitating increased expression of genes in response to stimuli. Here we systematically investigate this model using computational analyses. RESULTS: We use CTCF ChIA-PET data from the ENCODE project to show that CTCF-associated chromatin loops have a tendency to enclose regions of enhancer-regulated stimulus responsive genes, insulating them from neighboring regions of constitutively expressed housekeeping genes. To facilitate cell type-specific CTCF loop identification, we develop an algorithm to predict CTCF loops from ChIP-seq data alone by exploiting the CTCF motif directionality in loop ...
Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion ...
Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion ...
Renaud Dumas. Significance: In most biological processes, genes have to be activated and/or repressed. In plants, the TOPLESS protein is essential for gene repression through its action as a corepressor bridging transcription factor with chromatin remodeling complexes. Here we combine biochemical and structural studies to describe the structure of TOPLESS, how it tetramerizes, and how it interacts with its protein partners. We show that both the tetramerization interface and the binding site for protein partners have been conserved since algae, highlighting the ancestrality of TOPLESS function. Comparison of this plant protein with one of its animal counterparts also shows how corepressors can use a common domain differently to achieve similar properties, illustrating the tinkering of evolution in transcriptional repression.. Abstract: Transcriptional repression involves a class of proteins called corepressors that link transcription factors to chromatin remodeling complexes. In plants such as ...
This model represents the amino-terminal helix-turn-helix repressor region of the biotin--acetyl-CoA-carboxylase ligase/biotin operon repressor bifunctional protein BirA. In many species, the biotin--acetyl-CoA-carboxylase ligase ortholog lacks this DNA-binding repressor region and therefore is not equivalent to the well-characterized BirA of E. coli. This HMM may recognize some other putative repressor proteins, such as DnrO of Streptomyces peucetius with scores below the noise cutoff but with significance shown by low E-value ...
We have generated an extensive genetic map of functionally allowed and/or structurally allowed amino acid substitutions in Arc repressor, a DNA binding protein of unknown structure. Analysis of the allowed substitution patterns identifies residues that are likely to be involved in protein function and identifies side chains that play important structural roles, including residues likely to form the hydrophobic core. The identities of approximately one-third of the residues in Arc repressor are functionally important, about one-half are structurally important, and the remainder are unimportant for either structure or function. The patterns of obligatory hydrophobic positions permit strong predictions of secondary structure. Study holds ProTherm entries: 831, 832, 833, 834, 835, 836, 837, 838, 839, 840, 841, 842, 843, 844, 845, 846, 847, 848, 849 Extra Details: Arc repressor; DNA binding; protein folding;,secondary structure prediction; mutagenesis. ...
Sin3 forms the scaffold for a multiprotein corepressor complex that silences transcription via the action of histone deacetylases. Sin3 is recruited to the DNA by several DNA binding repressors, such as the helix-loop-helix proteins of the Mad family. Here, we elaborate on the Mad-Sin3 interaction based on a binding study, solution structure, and dynamics of the PAH2 domain of mSin3 in complex to an extended Sin3 interacting domain (SID) of 24 residues of Mad1. We show that SID residues Met7 and Glu23, outside the previously defined minimal binding motif, mediate additional hydrophobic and electrostatic interactions with PAH2. On the basis of these results we propose an extended consensus sequence describing the PAH2-SID interaction specifically for the Mad family, showing that residues outside the hydrophobic core of the SID interact with PAH2 and modulate binding affinity to appropriate levels ...
negative regulation of Ras protein signal transduction (GO:0046580) ; negative regulation of S phase of mitotic cell cycle (GO:0045749) ; negative regulation of transcription (GO:0016481) ; nucleus (GO:0005634) ; transcriptional repressor activity (GO:0016564) ; transcription factor activity (GO:0003700) ; regulation of cell cycle (GO:0000074) ; regulation of transcription, DNA-dependent (GO:0006355) ; transcription factor complex (GO:0005667 ...