B lymphocyte-induced maturation protein-1 (Blimp-1) is a transcriptional repressor that plays an important role during plasmacytic differentiation and is expressed in normal and transformed plasma cells. We here investigated the importance of continuous Blimp-1 expression. We found that knockdown of Blimp-1 expression by lentiviral vector-delivered short hairpin RNA causes apoptosis in multiple myeloma cell lines and plasmacytoma cells$ indicating that continued expression of Blimp-1 is required for cell survival. ...
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TY - JOUR. T1 - Transcriptional repressor REST drives lineage stage-specific chromatin compaction at Ptch1 and increases AKT activation in a mouse model of medulloblastoma. AU - Dobson, Tara H.W.. AU - Tao, Rong Hua. AU - Swaminathan, Jyothishmathi. AU - Maegawa, Shinji. AU - Shaik, Shavali. AU - Bravo-Alegria, Javiera. AU - Sharma, Ajay. AU - Kennis, Bridget. AU - Yang, Yanwen. AU - Callegari, Keri. AU - Haltom, Amanda R.. AU - Taylor, Pete. AU - Kogiso, Mari. AU - Qi, Lin. AU - Khatua, Soumen. AU - Goldman, Stewart. AU - Lulla, Rishi R.. AU - Fangusaro, Jason. AU - MacDonald, Tobey J.. AU - Li, Xiao Nan. AU - Hawkins, Cynthia. AU - Rajaram, Veena. AU - Gopalakrishnan, Vidya. PY - 2019/1/22. Y1 - 2019/1/22. N2 - In medulloblastomas (MBs), the expression and activity of RE1-silencing transcription factor (REST) is increased in tumors driven by the sonic hedgehog (SHH) pathway, specifically the SHH- (children 3 to 16 years) and SHH- (infants) subgroups. Neuronal maturation is greater in SHH- than ...
Neuron-restrictive silencer factor regulates the N-methyl-D-aspartate receptor 2B subunit gene in basal and ethanol-induced gene expression in fetal cortical ne
Previous studies have identified the immunological functions of transcription factor B lymphocyte-induced maturation protein-1 (Blimp-1) in various adaptive immune cell types such as T and B lymphocytes. More recently, it has been shown that Blimp-1 extends its functional roles to dendritic cells (DCs) and macrophages, two cell types belonging to the innate immune system. The protein acts as a direct and indirect regulator of target genes by recruiting chromatin modification factors and by regulating microRNA expression, respectively. In DCs, Blimp-1 has been identified as one of the components involved in antigen presentation. Genome-wide association studies identified polymorphisms associated with multiple autoimmune diseases such as system lupus erythematosus, rheumatoid arthritis, and inflammatory bowel disease in PRDM1, the gene encoding Blimp-1 protein. In this review, we will discuss the immune regulatory functions of Blimp-1 in DCs with a main focus on the tolerogenic mechanisms of Blimp-1
In molecular genetics, a repressor is a DNA- or RNA-binding protein that inhibits the expression of one or more genes by binding to the operator or associated silencers. A DNA-binding repressor blocks the attachment of RNA polymerase to the promoter, thus preventing transcription of the genes into messenger RNA. An RNA-binding repressor binds to the mRNA and prevents translation of the mRNA into protein. This blocking of expression is called repression. If an inducer, a molecule that initiates the gene expression, is present, then it can interact with the repressor protein and detach it from the operator. RNA polymerase then can transcribe the message (expressing the gene). A corepressor is a molecule that can bind to repressor and make it bind to the operator tightly, which decreases transcription. A repressor that binds with a corepressor is termed an aporepressor or inactive repressor. One type of aporepressor is the trp repressor, an important metabolic protein in bacteria. The above ...
Transcription repressor. Molecular model of the Tup 1 transcription repressor protein. Transcription repressors bind to specific sequences of DNA (deoxyribonucleic acid) and prevent the transcription (transfer) of genetic information from DNA to RNA (ribonucleic acid). - Stock Image F006/9316
Compaction and looping of the ~2.5-Mb Igh locus during V(D)J rearrangement is essential to allow all V(H) genes to be brought in proximity with D(H)-J(H) segments to create a diverse antibody repertoire, but the proteins directly responsible for this are unknown. Because CCCTC-binding factor (CTCF) has been demonstrated to be involved in long-range chromosomal interactions, we hypothesized that CTCF may promote the contraction of the Igh locus. ChIP sequencing was performed on pro-B cells, revealing colocalization of CTCF and Rad21 binding at ~60 sites throughout the V(H) region and 2 other sites within the Igh locus. These numerous CTCF/cohesin sites potentially form the bases of the multiloop rosette structures at the Igh locus that compact during Ig heavy chain rearrangement. To test whether CTCF was involved in locus compaction, we used 3D-FISH to measure compaction in pro-B cells transduced with CTCF shRNA retroviruses. Reduction of CTCF binding resulted in a decrease in Igh locus ...
A central feature of broad host range IncP-1 plasmids is the set of regulatory circuits that tightly control plasmid core functions under steady-state conditions. Cooperativity between KorB and either KorA or TrbA repressor proteins is a key element of these circuits and deletion analysis has implicated the conserved C-terminal domain of KorAand TrbAin this interaction. By NMR we show that KorA and KorB interact directly and identify KorA amino acids that are affected on KorB binding. Studies on mutants showed that tyrosine 84 (or phenylalanine, in some alleles) is dispensable for repressor activity but critical for the specific interaction with KorB in both in vivo reporter gene assays and in vitro electrophoretic mobility shift and co-purification assays. This confirms that direct and specific protein-protein interactions are responsible for the cooperativity observed between KorB and its corepressors and lays the basis for determining the ...
Results We found that VEGF release from BMSCs was significantly increased in parallel with high level of HIF-1α in BMSCs following anoxia or hypoxia in time-dependent manner. Furthermore, the level of VEGF released from BMSCs overexpressing CREG and the expression of HIF-1α in BMSCs overexpressing CREG were higher than the normal BMSCs under hypoxia. Rather, HIF-1α steady-state mRNA was also affected by CREG. This effect was associated with constitutive activation of phosphatidylinositol 3-kinase (PI3K)/Akt and its effector p70 S6 kinase (p70S6K), but not extracellular-signal regulated kinase 1/2. The use of small molecule inhibitors LY294002 or rapamycin to inhibit PI3K/Akt and p70S6K activities, respectively, resulted in diminished HIF-1α activation and subsequent VEGF expression. RNA interference-mediated knockdown of HIF-1α suppressed CREG-induced VEGF synthesis and angiogenic tube formation, confirming that the effect was HIF-1α specific.. ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
In the present study, we demonstrate that ectopic expression of the corepressor protein Sin3 leads to stabilization of both transfected and endogenous p53. That this effect is a direct impact of interaction of Sin3 with p53 is supported by our finding that a 15-amino-acid deletion mutant of p53 that is incapable of interacting with Sin3 also fails to be stabilized by this protein. Similarly, mutation of proline 71 of p53 to arginine or leucine significantly impairs Sin3 binding and Sin3-mediated stabilization. These data support a tight correlation between Sin3 binding and stabilization of p53. Our data also indicate that stabilization by Sin3 is likely the result of inhibition of proteasome-mediated degradation of p53. Interestingly, unlike p14ARF, MDMX, and pRB, Sin3 does not require the presence of MDM2 for this effect. Therefore, these findings point to the existence of a potentially novel pathway for p53 stabilization that does not involve inhibition of MDM2 function.. At least two proteins ...
Squamous cell carcinoma (SCC) is a treatment-refractory subtype of human cancer arising from stratified epithelium of the skin, lung, esophagus, oropharynx and other tissues. A unifying feature of SCC is high-level expression of the p53 related protein p63 (TP63) in 80% of cases. The major p63 isoform expressed in SCC is ΔNp63α, an N-terminally truncated form which functions as a key SCC cell survival factor by mechanisms that are unclear. In this study we demonstrate that ΔNp63α associates with HDAC1 and HDAC2 to form an active transcriptional repressor complex that can be targeted to therapeutic advantage. Repression of pro-apoptotic Bcl-2 family member genes including PUMA by p63/HDAC is required for survival of SCC cells. Cisplatin chemotherapy, a mainstay of SCC treatment, promotes dissociation of p63 and HDAC from the PUMA promoter, leading to increased histone acetylation, PUMA activation and apoptosis. These effects are recapitulated upon targeting the p63/HDAC complex selectively ...
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The nonspecific DNA binding capacity of the lac repressor protein has been assessed by two different methods. Boundary sedimentation of repressor and calf thymus DNA fragmented by shearing yielded dissociation constants in good agreement with values previously reported in the literature. The b ...
Previously, this and other laboratories have identified two regions on YY1 that mediate transcriptional repression (1, 26-28). Herein, we have further delineated the repression domain in one region to amino acids 170-200. More important, we have identified a novel mammalian corepressor that interacts with this domain.. YY1, like most eukaryotic repressors described to date, seems to act directly on the general transcription machinery-a mechanism referred to as active repression (for review, see refs. 29-32). Three types of domains have been identified thus far in active transcriptional repressors (for review, see refs. 29-32): alanine-rich, glutamine-rich, and/or proline-rich. Currently, it is not known whether these repression domains function by contact with the general transcriptional machinery, and proteins that interact with these domains have yet to be identified. Inspection of the YY1 amino acid sequence has failed to reveal any resemblance to the primary sequence motifs that characterize ...
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5-CACGTG-3. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
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Repressors and activators are often allosteric proteins whose function is modified by ligand binding. In general, a ligand alters the conformation of the protein and affects its ability to bind to specific DNA sequences. For example, some repressors control the synthesis of enzymes for a catabolic pathway. In the absence of substrate for these enzymes, the genes are repressed. When substrate is present, it binds to the repressor, causing the repressor to dissociate from the DNA and allowing the genes to be transcribed. Ligands that bind to and inactivate repressors are called inducers because they induce transcription of the genes controlled by the repressors. In contrast, some repressors that control the synthesis of enzymes for a biosynthetic pathway bind to DNA only when associated with a ligand. The ligand is often the end product of the biosynthetic pathway. This regulatory mechanism ensures that the genes are turned off as product accumulates. Ligands that bind to and activate repressors ...
The galactose represser protein from E. coli has a pI of about 5.9. While purification protocols were being designed, it was found to bind to a Mono-S column at pH values of 7 and below. (Mono-S columns have S-type sulfonic acid.
In chapter 3, "The Sense of Sensibility," author Wendy Jones uses scenes from one of Jane Austens most celebrated novels to illustrate the functioning of the bodys stress response system.. 0 Comments. ...
1IGQ: An Src homology 3-like domain is responsible for dimerization of the repressor protein KorB encoded by the promiscuous IncP plasmid RP4.
Deposition date: 2010-06-21 Original release date: 2010-07-09. Authors: Goel, Anupam. Citation: Goel, Anupam; Tripet, Brian; Tyler, Robert; Nebert, Lucas; Copie, Valerie. "Backbone Amide Dynamics Studies of Apo-L75F-TrpR, a Temperature-Sensitive Mutant of the Tryptophan Repressor Protein (TrpR): Comparison with the (15)N NMR Relaxation Profiles of Wild-Type and A77V Mutant Apo-TrpR Repressors." Biochemistry 49, 8006-8019 (2010).. Assembly members: ...
General Repressor Of Transcription; Forms Complex With Cyc8p, Involved In The Establishment Of Repressive Chromatin Structure Through Interactions With Histones H3 And H4, Appears To Enhance Expression Of Some Genes
Although genetic evidence suggests that SNI1 is a transcriptional repressor (Li et al., 1999), its vanishingly low level of expression and lack of sequence homology with known proteins or domains have made the application of conventional biochemical and molecular methodologies ineffective. To remedy this, we developed a combination of genetic and genomic approaches to dissect the structure and function of this novel regulator.. The use of the GFP-SNI1 fusion protein allowed the observation of the subcellular localization of SNI1 in planta. Because of the extremely low protein levels and the background fluorescence of chlorophyll in green tissues, GFP-SNI1 fluorescence was visible only in roots. Although SAR is expressed only in the aerial parts of the plant, SNI1 is likely to be functional in roots because in the sni1 mutant, root development is substantially impaired. The GFP-SNI1 fusion complemented all of the sni1 phenotypes in both shoot and root tissues, indicating that the fusion protein ...
Complete information for MXD4 gene (Protein Coding), MAX Dimerization Protein 4, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The COP1/SPA E3 ubiquitin ligase is a general repressor of photomorphogenesis in the dark. The four SPA family members, SPA1-4, have been shown to have partially overlapping but distinct functions in response to light. SPA1 is a key player of repressing photomorphogenesis, while SPA2 only functions in the dark. SPA2, on one hand, is not as stable as SPA1 in the light. On the other hand, the COP1/SPA2 complex also loses its biological function in the light via unknown mechanisms. This functional divergence of SPA1 and SPA2 has been shown to depend on the differences between their protein sequences. By phenotypical studies of the transgenic seedlings expressing chimeric constructs, I will try to answer the question that which domain is responsible for the diverged function of SPA1 and SPA2. I will also attempt to reveal the molecular basis underlying this divergence. ...
Inducer does NOT bind DNA. Inducer binds either activator OR repressor. It makes activator BETTER able to bind DNA = more transcription OR it makes repressor LESS able to bind DNA = transcription ...
Prohibitin, an evolutionarily conserved gene situated on chromosome 17q21, was originally identified as a gene with antiproliferative properties. Studies of a Japanese population have shown prohibitin to be somatically mutated in a proportion of breast tumours. The gene has not heretofore been shown to have an association with inherited forms of breast or other cancers. In this thesis the technique of denaturing gradient gel electrophoresis (DGGE) was developed to analyse the complete coding sequence of the prohibitin gene from fragments generated by the polymerase chain reaction (PCR). This was achieved by examining the melting profiles of different regions of the prohibitin sequence with the melt map program MELT87. Four overlapping fragments were designed and subsequently amplified by reverse transcription PCR thus enabling analysis of the prohibitin cDNA sequence by DGGE. A further five fragments were developed for analysis of prohibitin from genomic DNA. These five fragments were generated ...
Complete information for ASXL3 gene (Protein Coding), Additional Sex Combs Like 3, Transcriptional Regulator, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Filtering by: Creator Maria A. Schumacher Remove constraint Creator: Maria A. Schumacher Degree Ph.D. Remove constraint Degree: Ph.D. Department Dept. of Biochemistry and Molecular Biology Remove constraint Department: Dept. of Biochemistry and Molecular Biology Keyword crystallography Remove constraint Keyword: crystallography Keyword repressor proteins Remove constraint Keyword: repressor proteins Keyword dna-binding proteins Remove constraint Keyword: dna-binding proteins Collection Scholars Archive Remove constraint Collection: Scholars Archive ...
cdna:known chromosome:VEGA66:2:153345845:153404007:1 gene:OTTMUSG00000019852 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Asxl1 description:additional sex combs like 1 (Drosophila ...
The inducer binds to the repressor protein and induces a conformational change so that the repressor does not bind to the operator DNA sequence ...
The repressor CytR and the activator CRP, two dimeric proteins, interact to form a complex repressor nucleoprotein in the intergenic region. When only CRP is bound to this promoter, it functions as an activator, and then, when CytR binds to DNA and to CRP, the activation is repressed because CytR masks an activating region of CRP that otherwise would contact the RNA polymerase to activate transcription ,CITS:[ 8736525][10766824],. The CytR protein cannot act alone; the synergistic DNA binding is increased by direct interaction with CRP ,CITS:[1962841][ 2170326][1649947],. At times CytR also repositions CRP to alternative DNA-binding sites that are not functional for activation ,CITS:[ 8736525 ...
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Plasmid pFUW-tetO-GFI1B from Dr. Filipe Pereiras lab contains the insert Growth Factor Independent 1B transcriptional repressor and is published in Cell Rep. 2018 Dec 4;25(10):2821-2835.e7. doi: 10.1016/j.celrep.2018.11.032. This plasmid is available through Addgene.
Signal transductionRegulatory functionsDNA interactionsGTP-sensing transcriptional pleiotropic repressor CodY (TIGR02787; HMM-score: 351.8) ...
PRDM1/Blimp1山羊多克隆抗体(ab106766)可与人样本反应并经WB, IHC, ChIP实验严格验证,被1篇文献引用。所有产品均提供质保服务,中国75%以上现货。
PRDM1/Blimp1兔多克隆抗体(ab119401)可与小鼠, 大鼠, 人样本反应并经WB实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
How is B Lymphocyte-Induced Maturation Protein abbreviated? BLIMP stands for B Lymphocyte-Induced Maturation Protein. BLIMP is defined as B Lymphocyte-Induced Maturation Protein somewhat frequently.
The Proline-Rich Homeodomain protein (PRH/Hex) is a transcription factor that functions as an important regulator of vertebrate development and many other processes in the adult including haematopoiesis. The Groucho/TLE family of co-repressor proteins also regulate development and modulate the activity of many DNA-binding transcription factors during a range of diverse cellular processes including haematopoiesis. We have shown previously that PRH is a repressor of transcription in haematopoietic cells and that an Eh-1 motif present within the N-terminal transcription repression domain of PRH mediates binding to Groucho/TLE proteins and enables co-repression. Here we demonstrate that PRH regulates the nuclear retention of TLE proteins during cellular fractionation. We show that transcriptional repression and the nuclear retention of TLE proteins requires PRH to bind to both TLE and DNA. In addition, we characterise a trans-dominant negative PRH protein that inhibits wild type PRH activity by ...
Upon EBV infection, mature human B cells become activated, grow and proliferate. In vivo, in the presence of T cells or T cell-derived factors, infected cells can enter the germinal centre and differentiate into memory B cells, the site of long-term EBV latency and persistence. However, it has not been established what happens if T cell help is unavailable (Th-ve). Usually in the absence of T cell help, antigen-activated B cells can enter the default plasma cell differentiation pathway, resulting in antibody-producing plasma cells. We suggest EBV has evolved to prevent default plasma cell differentiation, thus favouring latency in memory B cells, through specific repression of the plasma cell differentiation factors p18INK4c and B lymphocyte-induced maturation protein-1 (BLIMP-1), by the viral transcription factors EBNA3A and EBNA3C that act in vitro to support the activated B-blast population in establishing continuously proliferating lymphoblastoid cell lines (LCLs). Since the repression of ...
TY - JOUR. T1 - Components of the SMRT corepressor complex exhibit distinctive interactions with the POZ domain oncoproteins PLZF, PLZF-RAR∅, and BCL-6. AU - Wong, Chi Wai. AU - Privalsky, Martin L.. PY - 1998/10/16. Y1 - 1998/10/16. N2 - Many transcription factors function by repressing gene transcription. For a variety of these transcription factors the ability to physically recruit auxiliary proteins, denoted corepressors, is crucial for the ability to silence gene expression. We and others have previously implicated the SMRT corepressor in the actions of the PLZF transcription factor and in the function of its oncogenic derivative, PLZF-retinoic acid receptor (RARα), in promyelocytic leukemia. We report here that PLZF, and a structurally similar transcriptional repressor, BCL-6, can interact with a variety of corepressor proteins in addition to SMRT, including the mSin3A protein and (for PLZF) histone deacetylase-1. Unexpectedly, these additional interactions with corepressor components ...
View Notes - Handout16Activation from BIS 101 at UC Davis. Model 2: TRANSCRIPTIONAL REPRESSOR - repressor active mRNA____________ > + repressor X inactive repression of repressor active
Rabbit anti IRF8 antibody recognizes Interferon regulatory factor 8, (IRF8) also known as Interferon consensus sequence-binding protein (I
EN] Mot3 and Rox1 are transcriptional repressors of hypoxic genes. Both factors recently have been found to be involved in the adaptive response to hyperosmotic stress, with an important function in the adjustment of ergosterol biosynthesis. Here, we determine the gene expression profile of a mot3 rox1 double mutant under acute osmostress at the genomic scale in order to identify the target genes affected by both transcription factors upon stress. Unexpectedly, we find a specific subgroup of osmostress-inducible genes to be under positive control of Mot3. These Mot3-activated stress genes also depend on the general stress activators Msn2 and Msn4. We confirm that both Mot3 and Msn4 bind directly to some promoter regions of this gene group. Further-more, osmostress-induced binding of the Msn2 and Msn4 factors to these target promoters is severely affected by the loss of Mot3 function. The genes repressed by Mot3 and Rox1 preferentially encode proteins of the cell wall and plasma membrane. Cell ...
Transcription of genes encoding enzymes for the biosynthesis of methionine and trytophan in Escherichia coli is regulated by the ligand-activated met and trp repressors. X-ray crystallographic studies show how these two small proteins, although similar in size and function, have totally different three-dimensional structures and specifically recognize their respective DNA operator sequences in different ways. A common feature is that both repressors bind as cooperative arrays to tandem repeats of 8 base-pair Met or Trp boxes respectively, and the consensus sequences share the rare tetranucleotide CTAG. A series of structural and functional studies have shown how the two repressors discriminate between their operators, using a combination of direct contacts between side chains and bases, and indirect sensing of conformational properties of the DNA. ...
Specific wiring of gene-regulatory networks is likely to underlie much of the phenotypic difference between species, but the extent of lineage-specific regulatory architecture remains poorly understood. The essential vertebrate transcriptional repressor REST (RE1-Silencing Transcription Factor) targets many neural genes during development of the preimplantation embryo and the central nervous system, through its cognate DNA motif, the RE1 (Repressor Element 1). Here we present a comparative genomic analysis of REST recruitment in multiple species by integrating both sequence and experimental data. We use an accurate, experimentally validated Position-Specific Scoring Matrix method to identify REST binding sites in multiply aligned vertebrate genomes, allowing us to infer the evolutionary origin of each of 1,298 human RE1 elements. We validate these findings using experimental data of REST binding across the whole genomes of human and mouse. We show that one-third of human RE1s are unique to ...
Specific wiring of gene-regulatory networks is likely to underlie much of the phenotypic difference between species, but the extent of lineage-specific regulatory architecture remains poorly understood. The essential vertebrate transcriptional repressor REST (RE1-Silencing Transcription Factor) targets many neural genes during development of the preimplantation embryo and the central nervous system, through its cognate DNA motif, the RE1 (Repressor Element 1). Here we present a comparative genomic analysis of REST recruitment in multiple species by integrating both sequence and experimental data. We use an accurate, experimentally validated Position-Specific Scoring Matrix method to identify REST binding sites in multiply aligned vertebrate genomes, allowing us to infer the evolutionary origin of each of 1,298 human RE1 elements. We validate these findings using experimental data of REST binding across the whole genomes of human and mouse. We show that one-third of human RE1s are unique to ...
The majority of bacterial gene regulators bind as symmetric dimers to palindromic DNA operators of 12-20 base pairs (bp). Multimeric forms of proteins, including tetramers, are able to recognize longer operator sequences in a cooperative manner, although how this is achieved is not well understood due to the lack of complete structural information. Models, instead of structures, of complete tetrameric assembly on DNA exist in literature. Here we present the crystal structures of the multidrug-binding protein TtgV, a gene repressor that controls efflux pumps, alone and in complex with a 42-bp DNA operator containing two TtgV recognition sites at 2.9 Å and 3.4 Å resolution. These structures represent the first full-length functional tetrameric protein in complex with its intact DNA operator containing two continuous recognition sites. TtgV binds to its DNA operator as a highly asymmetric tetramer and induces considerable distortions in the DNA, resulting in a 60° bend. Upon binding to its ...