Title:Adult Stem Cells and Skeletal Muscle Regeneration. VOLUME: 15 ISSUE: 4. Author(s):Domiziana Costamagna, Emanuele Berardi, Gabriele Ceccarelli and Maurilio Sampaolesi. Affiliation:Translational Cardiomyology Laboratory, Stem Cell Institute Leuven, KU Leuven, Belgium.. Keywords:Adult stem cells, Muscular dystrophy, Skeletal muscle regeneration.. Abstract:Satellite cells are unipotent stem cells involved in muscle regeneration. However, the skeletal muscle microenvironment exerts a dominant influence over stem cell function. The cell intrinsic complexity of the skeletal muscle niche located within the connective tissue between fibers includes motor neurons, tendons, blood vessels, immune response mediators and interstitial cells. All these cell types modulate the trafficking of stimuli responsible of muscle fiber regeneration. In addition, several stem cell types have been discovered in skeletal muscle tissue, mainly located in the interstitium. The majority of these stem cells appears to ...

2016 Annual Meeting: Extracellular Matrix Impregnated with Adipose Derived Stem Cells in Skeletal Muscle Regeneration following Volumetric Muscle Loss in a Murine Model

Ellex Medical Lasers, a provider in the design and manufacture of ophthalmic laser and ultrasound systems, has presented positive clinical results for its laser Retina Regeneration Therapy (Ellex 2RT) for the treatment of age-related macular degeneration (AMD) and diabetic retinopathy (DR), two conditions that usually result in irreversible blindness for sufferers. Ellex 2RT treatment involves a laser device designed and manufactured by Ellex which delivers a controlled nanosecond dose of laser energy into the eye.. The company said that the clinical trials of Ellex 2RT demonstrate the potential to reverse the degenerative processes caused by the disease AMD through the reduction of yellow deposits known as drusen, present in the retinal tissue of people suffering from AMD.. Ellex 2RT offers the potential to treat AMD in its early stages, before vision is lost. Until now treatment has been restricted to targeting late-stage complications associated with the wet form of AMD, which only accounts ...

Gyöngyösi, Mariann; Wojakowski, Wojciech; Navarese, Eliano P; Moye, Lemuel À (2016). Meta-Analyses of Human Cell-Based Cardiac Regeneration Therapies : Response to Gyöngyösi, Wojakowski, Navarese, Moyé, and the ACCRUE Investigators. Circulation Research, 118(8):1254-1263. ...

TY - JOUR. T1 - Skeletal muscle regeneration in Xenopus tadpoles and zebrafish larvae. AU - Rodrigues, Alexandre Miguel Cavaco. AU - Christen, Bea. AU - Martí, Mercé. AU - Izpisua Belmonte, Juan Carlos. PY - 2012/4/3. Y1 - 2012/4/3. N2 - Background: Mammals are not able to restore lost appendages, while many amphibians are. One important question about epimorphic regeneration is related to the origin of the new tissues and whether they come from mature cells via dedifferentiation and/or from stem cells. Several studies in urodele amphibians (salamanders) indicate that, after limb or tail amputation, the multinucleated muscle fibres do dedifferentiate by fragmentation and proliferation, thereby contributing to the regenerate. In Xenopus laevis tadpoles, however, it was shown that muscle fibres do not contribute directly to the tail regenerate. We set out to study whether dedifferentiation was present during muscle regeneration of the tadpole limb and zebrafish larval tail, mainly by cell ...

Apparatuses, systems, and methods for enhancing bone or soft tissue regeneration are provided. For example, a conduit, having one or more segments, can originate at a tissue regeneration site and can have a first opening to promote physiological signals to enter the conduit and transit to a second opening that penetrates a histologically rich source of multipotent mesenchymal cells, promoting the multipotent mesenchymal cells to produce tissue regeneration response products, the response products transiting through the second opening to egress at the first opening of the conduit, and promoting tissue regeneration at the tissue regeneration site. Transit of the physiological signals and the tissue regeneration response products is promoted through physical and/or chemical means, as is promotion of the anatomical functionality of the regenerated tissue. For example, a number of the segments of the conduit can be formed at least partially from an osteoceramic material.

Tissue engineering and organ regeneration market encompasses those products used in medicine. The application of these new age biomaterials include burn treatment or wound care, orthopaedics, urological products, and neurology products. Engineered cell growth has been present over the past many years with little or no applications in medicine. Recent developments and progress in the field of stem cell technology, cell culture technology and immunopassivation and immunomodulation has provided incredible opportunities to market players. Several cardiovascular applications such as heart valve and myocardial tissue regeneration is also expected to provide excellent sustainability for the growth of the market. Rising cost of organ transplantation, long waiting lines and rising medical applications of 3D printing in tissue and organ regeneration will be the major growth drivers for this market in the near future.. Browse the full report with request TOC at ...

Here, we used classical genetics in zebrafish to identify temperature-sensitive mutants in caudal fin regeneration. Gross morphological, histological, and molecular analyses revealed that one of these strains, emmental (emm), failed to form a functional regeneration blastema. Inhibition of emm function by heat treatment during regenerative outgrowth rapidly blocked regeneration. This block was associated with reduced proliferation in the proximal blastema and expansion of the nonproliferative distal blastemal zone. Positional cloning revealed that the emm phenotype is caused by a mutation in the orthologue of yeast sly1, a gene product involved in protein trafficking. sly1 is upregulated in the newly formed blastema as well as during regenerative outgrowth. Thus, sly1 is essential for blastemal organization and proliferation during two stages of fin regeneration ...

For the longest time people have been exploring the frontiers of science and technology to look for materials and means to maintain health, to prevent and treat diseases and to live a long, productive life. Modern medicine has contributed significantly in the shortening the distance for reaching theses goals. However, merely treating the diseases by suppressing the symptoms or by inhibiting the infection of exogenous pathogens is often insufficient to completely restore physiological structure and function to the body as the tissue and organ has already been damaged. Fundamentally, the key to reaching this goal-restoration of physiological structure and function-lies in the regeneration of damaged tissues and organs in the face of injury, diseases, and aging.. What is regeneration actually? Regeneration maintains or restores the original, physiological structure and function of the tissues and organs in the body. Stocum (2006) Regenerative Biology & Medicine page 1, Academic Press. Regeneration ...

Cheapest Cartilage Regeneration Stem Cell Therapy price in Chennai, India is $6000. Average Cartilage Regeneration Stem Cell Therapy cost $8000, where prices can go as high as $10000. PlacidWay Medical Tourism provides cost comparison for Cartilage Regeneration Stem Cell Therapy, Stem Cell Therapy Prices in Chennai, India. Explore Cartilage Regeneration Stem Cell Therapy prices worldwide.

Greengate is the medieval heart of the city of Salford and lies on the Salford - Manchester border. Greengate has been a key investment focus of the council for a number of years now, with a vision to create a new corporate centre for Salford.. There are still a number of key opportunities for further development within the area and Salford City Council therefore commissioned Urban Vision (planning consultants), and Feilden Clegg Bradley Studios, (architects and masterplanners) to update the regeneration strategy during 2017. We have now reached a stage where we wish to consult on the new draft regeneration strategy (at the bottom of the page).. We are undertaking an eight week consultation on the draft regeneration strategy starting on 27 November 2017.. As part of the consultation, we will be holding a drop-in session on the 13 December 2017 at Block 12, Spectrum, Blackfriars Road, Salford between 1pm and 7pm (access from Blackfriars Road). This will be an opportunity to meet officers from ...

TY - JOUR. T1 - Macrophages are required for neonatal heart regeneration. AU - Aurora, Arin B.. AU - Porrello, Enzo R.. AU - Tan, Wei. AU - Mahmoud, Ahmed I.. AU - Hill, Joseph A.. AU - Bassel-Duby, Rhonda. AU - Sadek, Hesham A.. AU - Olson, Eric N.. PY - 2014/3/3. Y1 - 2014/3/3. N2 - Myocardial infarction (MI) leads to cardiomyocyte death, which triggers an immune response that clears debris and restores tissue integrity. In the adult heart, the immune system facilitates scar formation, which repairs the damaged myocardium but compromises cardiac function. In neonatal mice, the heart can regenerate fully without scarring following MI; however, this regenerative capacity is lost by P7. The signals that govern neonatal heart regeneration are unknown. By comparing the immune response to MI in mice at P1 and P14, we identified differences in the magnitude and kinetics of monocyte and macrophage responses to injury. Using a cell-depletion model, we determined that heart regeneration and ...

US funding for research into cardiac regeneration using stem cells is coming to an end after a five-year investigation revealed over 30 studies in this field must be retracted. In October 2018, The New York Times reported that Harvard and Bringham and ...

Regeneration research is more focused on translational values. However, lying at its very foundation is an understanding of how tissues and organs repair and renew themselves at the cellular level. The past decade has witnessed paradigm changing advances in regenerative biology, many of these stems from novel insights into stemness, pluripotency, cell death and their related intra- and inter-cellular biochemical and molecular processes. Some of these new insights are highlighted in the paragraphs that follow. We now have a much better understanding of how regeneration occurs in lower organisms. We have also discovered tools and means of nuclear reprogramming to generate induced pluripotency and changes in cell fate in mammalian models. With further research, there is reasonable hope that various obstacles of regeneration in humans can be better understood and tackled. As regeneration research enters a new era, CellBio welcomes timely review articles and original papers on the theme of

c-MET Regulates Myoblast Motility and Myocyte Fusion during Adult Skeletal Muscle Regeneration. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.

This work studies Ni-based catalyst deactivation and regeneration processes in the presence of H2S under a biogas tri-reforming process for hydrogen production, which is an energy vector of great interest. 25 ppm of hydrogen sulfide were continuously added to the system in order to provoke an observable catalyst deactivation, and once fully deactivated two different regeneration processes were studied: a self-regeneration and a regeneration by low temperature oxidation. For that purpose, several Ni-based catalysts and a bimetallic Rh-Ni catalyst supported on alumina modified with CeO2 and ZrO2 were used as well as a commercial Katalco 57-5 for comparison purposes. Ni/Ce-Al2O3 and Ni/Ce-Zr-Al2O3 catalysts almost recovered their initial activity. For these catalysts, after the regeneration under oxidative conditions at low temperature, the CO2 conversions achieved-79.5% and 86.9%, respectively-were significantly higher than the ones obtained before sulfur poisoning-66.7% and 45.2%, respectively. This

Background: Epidemiological studies related poor maternal nutrition and subsequent growth retardation in the progeny to the development of diabetes later in life. Low-protein diet during gestation altered the beta-cell development of the rat progeny by decreasing beta-cell proliferation and increasing their sensitivity to nitric oxide and cytokines in the foetus. This disturbed maternal environment had long-lasting consequences because the higher beta-cell vulnerability was maintained at adulthood. Aim: The aim of this study was to determine whether early malnutrition influences the vulnerability and the regeneration capacity of beta-cells after streptozotocin (STZ) damage at adulthood. Methods: Gestating rats were fed either a control or a low-protein diet until weaning. Adult female offspring received injections of Freunds adjuvant weekly for 5 weeks followed 24 h later by STZ. Half of the cohort was killed at d34, whereas the other half was maintained until d48 to analyse the regeneration ...

The pancreas is a vertebrate-specific endodermal organ executing major functions in food digestion and glucose homeostasis. The mature organ is composed of an exocrine compartment with acinar and duct cells that produce and transport digestive enzymes into the gut, and an endocrine compartment from which metabolism-regulating peptide hormones including insulin are secreted into the blood stream. Studies on pancreas regeneration have focused mainly on the endocrine compartment, with the aim of gaining knowledge on β-cell regeneration. More recently, exocrine pancreas regeneration has been receiving more attention in the context of cancer and diabetes research because experimental models revealed a high capacity for cell fate plasticity (Murtaugh and Keefe, 2015).. Regeneration of exocrine tissue has been mainly studied in mouse models, where the loss of cells can be induced by caerulein treatment or partial duct ligation (Aghdassi et al., 2011; Lerch and Gorelick, 2013). Using these procedures, ...

Austin, TX (Vocus) November 4, 2010 -- Back pain or neck pain that isnt quickly resolved with the help of painkillers or physical therapy can be debilitating,

A group of researchers at Osaka University developed a synthetic tissue using synovium-derived mesenchymal stem cells (MSCs) for treating damaged cartilage, which had previously been incurable and had no effective therapies.

Since receiving injections of her own adult (autologous) stem cells in April, my dog Sheba has been steadily growing stronger, overcoming the pain of the crippling arthritis...

In humans, most cardiovascular disorders lead to the destruction of cardiac tissue which will be replaced by fibrosis, leading to arrhythmia and reduced contractile function, resulting in an increase in ventricular load. In order to maintain an overall cardiac output, cardiomyocytes undergo hypertrophic response, leading to pathological hypertrophy and heart failure. This increase in ventricular load, have to be sensed by mechanosensors such as the mechanosensitive ion channels such as TREK-1. Unlike mammals, adult zebrafish (zf) can fully regenerate their heart after an extensive insult through cardiomyocyte dedifferentiation followed by proliferation. We believe that in adult mammals, cardiomyocyte proliferation has been blocked/inhibited. Therefore its likely that genes which respond to increased ventricular load in mammals and trigger pathological hypertrophy will trigger cardiomyocyte proliferation during heart regeneration in zf. In this study we show that zTREK1a and zTREK1b have similar

article: Loss of Thy-1 may reduce lung regeneration after pneumonectomy in mice - Minerva Medica 2020 Jul 20 - Minerva Medica - Journals

Towards Minimally Invasive Exosome Therapies for Internal Organ Regeneration - posted in News: Targeted delivery of therapeutics remains one of the thorny issues in medical development. Everyone wants a way to deliver high doses of a therapeutic to a specific location in the body without it also ending up everywhere else. The major issue is that systemic administration will send the majority of whatever is injected into the body into the liver and lungs, and that limits the dose that can b...

Researchers at Weill Cornell Medical College, and colleagues, say they have taken an important step forward in their quest to turn on lung regeneration -- an advance that could effectively treat millions of people suffering from respiratory disorders. In the October 28, 2011 issue of Cell, the research team reports that they have uncovered the biochemical signals in mice that trigger generation of new lung alveoli, the numerous, tiny, grape-like sacs within the lung where oxygen exchange takes place. Specifically, the regenerative signals originate from the specialized endothelial cells that line the interior of blood vessels in the lung. While it has long been known that mice can regenerate and expand the capacity of one lung if the other is missing, this study now identifies molecular triggers behind this process, and the researchers believe these findings are relevant to humans. Several adult human organs have the potential upon injury to regenerate to a degree, and while we can readily ...

Macrophages play an essential role in the resolution of tissue damage through removal of necrotic cells, thus paving the way for tissue regeneration. Macrophages also directly support the formation of new tissue to replace the injury, through their acquisition of an anti-inflammatory, or M2, phenotype, characterized by a gene expression program that includes IL-10, the IL-13 receptor, and arginase 1. We report that deletion of two CREB-binding sites from the Cebpb promoter abrogates Cebpb induction upon macrophage activation. This blocks the downstream induction of M2-specific Msr1, Il10, II13ra, and Arg-1 genes, whereas the inflammatory (M1) genes Il1, Il6, Tnfa, and Il12 are not affected. Mice carrying the mutated Cebpb promoter (betaDeltaCre) remove necrotic tissue from injured muscle, but exhibit severe defects in muscle fiber regeneration. Conditional deletion of the Cebpb gene in muscle cells does not affect regeneration, showing that the C/EBPbeta cascade leading to muscle repair is muscle

TY - JOUR. T1 - Macrophage stimulating protein may promote tubular regeneration after acute injury. AU - Cantaluppi, Vincenzo. AU - Biancone, Luigi. AU - Romanazzi, Giuseppe Mauriello. AU - Figliolini, Federico. AU - Beltramo, Silvia. AU - Galimi, Francesco. AU - Camboni, Maria Gavina. AU - Deriu, Elisa. AU - Conaldi, Piergiulio. AU - Bottelli, Antonella. AU - Orlandi, Viviana. AU - Herrera, Maria Beatriz. AU - Pacitti, Alfonso. AU - Segoloni, Giuseppe Paolo. AU - Camussi, Giovanni. PY - 2008/10. Y1 - 2008/10. N2 - Macrophage-stimulating protein (MSP) exerts proliferative and antiapoptotic effects, suggesting that it may play a role in tubular regeneration after acute kidney injury. In this study, elevated plasma levels of MSP were found both in critically ill patients with acute renal failure and in recipients of renal allografts during the first week after transplantation. In addition, MSP and its receptor, RON, were markedly upregulated in the regenerative phase after glycerol-induced tubular ...

Principal Investigator：Toyoda Kyosuke, Project Period (FY)：2015-08-28 - 2017-03-31, Research Category：Grant-in-Aid for Research Activity start-up, Research Field：Periodontology

A mammal model of true organ regeneration may help scientists better understand this process so that they can prompt the regeneration of human organs.

An abundance of data across several vertebrate taxon groups (3, 11, 12) suggests that nerves may have a conserved evolutionary role that is essential to, or permissive for, limb regeneration. Despite the absence of innervation to the hind limb throughout the time of regeneration, the mouse model replaces the amputated digit tip with surprising fidelity in most tissue types. Our comprehensive examination at single-cell levels of primary hind limb tissues during normal maintenance, regeneration, and wound healing reveals that many presumed tissue stem/progenitor cells maintain clonal and differentiation capabilities in the absence of nerve supply.. In salamander embryos, removal of a large section of the neural tube leads to limb development without innervation. These limbs can be transplanted to the flank of a normal larva, where they can regenerate in the absence of nerve supply (33). This lack of nerve dependence is made possible by the substitution of nerve-derived factors from ectodermal ...

Researchers at Penn State have developed an innovative technology to regenerate functional neurons after brain injury and also in model systems used for research on Alzheimers disease.

New procedure repairs severed nerves in minutes, restoring limb use in days or weeks The team studied the mechanisms all animal cells use to repair damage to their membranes and focused on invertebrates, which have a superior ability to regenerate nerve axons compared to mammals. An axon is a long extension arising from a nerve…

New research in mice and rats, conducted at Wake Forest Baptist Medical Centers Institute for Regenerative Medicine, suggests that in body regeneration of muscle tissue might be possible by harnessing the bodys natural healing powers.. Reporting online ahead of print in the journal Acta Biomaterialia, the research team demonstrated the ability to recruit stem cells that can form muscle tissue to a small piece of biomaterial, or scaffold that had been implanted in the animals leg muscle. The secret to success was using proteins involved in cell communication and muscle formation to mobilize the cells.. Working to leverage the bodys own regenerative properties, we designed a muscle-specific scaffolding system that can actively participate in functional tissue regeneration, said Sang Jin Lee, Ph.D., assistant professor of regenerative medicine and senior author. This is a proof-of-concept study that we hope can one day be applied to human patients. ...

The mammalian heart has generally been considered to lack the ability to repair itself after injury, but a 2011 study in new-born mice challenged this view, providing evidence for complete regeneration after resection of 10% of the apex. However, in this study researchers attempted to replicate these recent findings but failed to uncover any evidence of complete heart regeneration in new-born mice that underwent apex resection.

In response to a severe injury that destroys a majority of the tissue, skeletal muscle is capable of complete regeneration and restoration of the cytoarchitecture within a 2‐week period (Miller et al, 1999; Seale and Rudnicki 2000; Shi and Garry 2006). This remarkable regenerative capacity is due to a rare population of cells termed myogenic progenitor cells that are resident in adult skeletal muscle. Although intense interest has been directed toward muscle regeneration, the molecular mechanisms that direct the MPC population are incompletely defined. In the present study, we have begun to decipher the MPC regulatory program by making three principal findings that enhance our mechanistic understanding of the MPC population. First, we utilized transgenic technology to define modular sequences of the Foxk1 gene that directs lacZ reporter expression to the MPC population. We have previously demonstrated that Foxk1 is restricted to the MPC population in adult skeletal muscle (Garry et al, 1997, ...

Before the new study, scientists did not know if all cardiomyocytes in the zebrafish heart shared the same regenerative ability or if they were equally able to regenerate all zones of the heart muscle. Cell plasticity, the ability of cells to convert themselves into another cell type, is frequently observed during embryonic development, but has never before been reported during tissue regeneration in an adult organism. In the study, which received funding from the European Research Council (ERC Starting Grant 2013 337703 zebraHeart), the authors investigated two types of cardiomyocyte, one localized in the innermost heart regions, the trabeculae, and the other in the exterior heart wall. Scientists had presumed that during regeneration each cardiomyoctye population would give rise only to the same specialized cell type. But the CNIC study shows that cardiomyocytes from the trabeculae can contribute to the regeneration of the external heart wall. The researchers conclude that their results ...

Direct regenerations by using mature cotyledonary node as a explants has been shown to be time-saving and convenient strategy for micropropagation of soybean. So we have evaluated regeneration protocol through single shoot using cotyledonary node as a rapid and efficient protocol for two soybean cultivars and one mutant line. Cotyledonary nodes explants obtained from 7-days-old in vitro seedlings. After 28 days, the percent of regeneration and after 42 days, regeneration area were calculated. The results showed that percent of regeneration and regeneration area of mutant line was significantly more than two cultivars, L17 and Williams. After shoot induction, plants were transferred to shoot elongation medium followed by transferring plants to rooting medium. The results showed that the percent of rooting was not significantly affected by genotypes. In another experiment to test kanamycin sensitivity of regenerated shoots, it was found that kanamaycin with 150 mg/L concentration is lethal for

In an earlier post, I pointed out the problems with trying to do away with the term regeneration by attempting to subsume regeneration under the category of effectual calling. But while I agree regeneration is a distinct (note: not separate) act from effectual calling, I agreed that it was through Gods Word in effectual calling that regeneration happens.. In his article on the topic however, John Murray goes further, trying to distinguish between two types of regeneration. The first is unmediated by the proclaimed Word but purely of divine fiat. The second is mediated by the proclaimed Word (pp. 196-7). In a certain sense, we can say that some people seem to show signs of regeneration prior to the proclamation of the Gospel, but is that a real example of someone who is regenerated by divine fiat alone apart from the proclaimed Word?. It is my contention that Murray is wrong here. While certainly there is a distinction between the divine fiat Word, and the proclaimed Word, yet, inasmuch as the ...

Regeneration is the ability to restore lost or damaged tissues, organs or limbs. It is a common feature in invertebrates, but far more limited in most vertebrates. Nevertheless, even humans possess some degree of regeneration ability. Children under 6 years of age are capable of regenerating lost fingertips and the human liver retains its ability to regenerate throughout a persons lifetime. Aside from being used to generally describe any number of specific healing processes, regeneration also is a specific method of healing that is noted for its ability to regrow lost limbs, severed nerve connections, and other wounds. This is present in some animals such as the newt, hydra, and a type of mouse. [1] [2]. With the exception of the MRL mice, mammals do not in general have the ability to regenerate. If the processes behind regeneration are fully understood, it is believed this would lead to better treatment for individuals with nerve injuries, broken backs, paralysis, and missing limbs ...

Researchers from Boston Childrens Hospital have discovered that the Lin28 gene, which is linked to Wilms tumor, may be the key to kidney regeneration.

To investigate losartans role in muscle injury regeneration in the context of aging, the Hopkins team worked with 40 mice which, at 21-months old, were considered geriatric. The researchers examined the stained muscle tissue under a microscope at four days and again at 19 days, looking for signs of regeneration: small fibers with larger-than-usual nuclei.. After four days, they saw no difference in the number of regenerating fibers between the losartan-treated mice and those not treated. However, after 19 days, the losartan-treated mice had about 10 to 15 per cent of scar tissue formation compared with 30 to 40 per cent of scar tissue formation in those not treated.. Next, the researchers conducted disuse experiments to find out if losartan, in addition to improving muscle regeneration, might have even broader clinical applications in the protection against immobilization atrophy.. Again, using 21-month-old mice, half treated with losartan and half not, the team this time clipped the hind right ...

Most metazoans have at least some ability to regenerate damaged cells and tissues, although the regenerative capacity varies depending on the species, organ, or developmental stage. Cell replacement and regeneration occur in two contexts: renewal of spent cells during tissue homeostasis (homeostatic growth), and in response to external injury, wounding, or amputation (epimorphic regeneration). Model organisms that display remarkable regenerative capacity include amphibians, planarians, Hydra, and the vertebrate liver. In addition, several mammalian organs, including the skin, gut, kidney, muscle, and even the human nervous system, have some ability to replace spent or damaged cells. Although the regenerative response is complex, it typically involves the induction of new cell proliferation through formation of a blastema, followed by cell specification, differentiation, and patterning. Stem cells and undifferentiated progenitor cells play an important role in both tissue homeostasis and tissue ...

Using single-cell RNA sequencing, researchers identified cells in mouse and human prostate tissues that appear to help regrow the gland after prostate cancer treatment.

After myocardial infarction in the mammalian heart, millions of cardiomyocytes are lost and replaced by fibrotic scar tissue. While fibrosis is persistent in adult mammals, there are some vertebrates, including zebrafish, with the capacity for regeneration. This process does not occur in the absence of fibrosis. Here we studied subpopulations of collagen-producing cells and analyzed their fate after complete regeneration of the zebrafish myocardium. Our data show that fibroblasts persisted in the regenerated heart but shut down the profibrotic program. While fibrosis could be considered as detrimental to the regeneration process, our study reveals a positive effect on cardiomyocyte proliferation. Accordingly, a fibrotic response can be beneficial for heart regeneration. In the zebrafish (Danio rerio), regeneration and fibrosis after cardiac injury are not mutually exclusive responses. Upon cardiac cryoinjury, collagen and other extracellular matrix (ECM) proteins accumulate at the injury site. However,

ECM remodeling is a critical step in development, wound healing and regeneration (31, 38, 40, 41, 60). The present study characterized the ECM composition and its changes during zebrafish regeneration. We have developed a decellularization protocol for zebrafish ventricles that results in ECM enrichment, as well as facilitating the analysis of the proteomic profile of the zebrafish ventricle ECM. Moreover, we have analyzed the ECM changes during heart regeneration and assessed the stiffness of the ECM at different time points of this process. Altogether, the results from our studies should help better understanding the role of the ECM in zebrafish heart regeneration.. The ECM composition has not been fully studied and described in the specific contexts of the zebrafish heart and cardiac regeneration. Few are the studies done to analyze the ECM in the zebrafish heart. The proteome of different zebrafish organs, including the heart, were analyzed by Abramsson and colleagues and only 4 collagen ...

Fibroblast growth factors (Fgfs) regulate critical biological processes such as embryonic development, tissue homeostais, wound healing, and tissue regeneration. In zebrafish, Fgf signaling plays an important role in the regeneration of the spinal cord, liver, heart, fin, and photoreceptors, although its exact mechanism of action is not fully understood. Utilizing an adult zebrafish extraocular muscle (EOM) regeneration model, we demonstrate that blocking Fgf receptor function using either a chemical inhibitor (SU5402) or a dominant-negative transgenic construct (dnFGFR1a:EGFP) impairs muscle regeneration. Adult zebrafish EOMs regenerate through a myocyte dedifferentiation process, which involves a muscle-to-mesenchyme transition and cell cycle reentry by differentiated myocytes. Blocking Fgf signaling reduced cell proliferation and active caspase 3 levels in the regenerating muscle with no detectable levels of apoptosis, supporting the hypothesis that Fgf signaling is involved in the early ...

Limb regeneration is observed in certain members of the animal phyla. Some animals keep this ability during their entire life while others lose it at some time during development. How do animals regenerate limbs? Is it possible to find unifying, conserved mechanisms of limb regeneration or have diff …

The director of the National Institutes of Health, Francis S. Collins, has announced that Pak Kin Wong, UA professor of aerospace and mechanical engineering and a BIO5 Institute member, has won a $1.5 million NIH Directors New Innovator Award.

Zebrafish heart regeneration relies on the capacity of cardiomyocytes to proliferate upon injury. To understand the principles of this process after cryoinjury-induced myocardial infarction, we established a spatio-temporal map of mitotic cardiomyocytes and their differentiation dynamics. Immunodete …

2. On the 17th December 2012, I reported to Cabinet the release of the consultation document Vibrant and Viable Places - A New Regeneration Framework. That report also recommended a response to that consultation (Cabinet Minute C1949 refers). 3. That report sets the context for current and future Welsh Government area based programmes with regeneration implications and that context is not repeated here. However, particularly relevant is the fact that the Barry Regeneration Area programme was launched by the Welsh Government in March 2010. A total of £9.8m of Welsh Government investment was allocated to the programme, which was initially intended to run for three years (April 2010 - March 2013 inclusive). However, following the spending review by the United Kingdom Government, this allocation was re-profiled over four years. Cabinet considered an update report on the Barry Regeneration Area Programme and the Welsh Governments Ministerial review of approaches to regeneration on 23rd July 2012 ...