Photoaffinity labeling analysis of the interaction of pituitary adenylate-cyclase-activating polypeptide (PACAP) with the PACAP type I receptor -- Cao et al. 244 (2): 400 -- FEBS Journal: YJ Cao, E Kojro, G Gimpl, M Jasionowski, F Kasprzykowski, L Lankiewicz and F Fahrenholz Max-Planck-Institut fur Biophysik, Frankfurt am Main, Germany. To identify residues and domains of…
MC1R encodes the protein known as melanocyte-stimulating hormone receptor (MSHR), a G protein-coupled receptor that binds to a class of pituitary peptide hormones known as the melanocortins.
Glycosaminoglycans (GAGs) contribute to the cellular uptake of cationic cell-penetrating peptides (CPPs). However, molecular details about the contributions of GAGs in CPP internalization remain unclear. In this study, we examined the cellular uptake mechanism of the arginine-rich CPP pituitary adenylate-cyclase-activating polypeptide (PACAP). We observed that the uptake efficacy of PACAP is dependent on the expression of cell surface GAGs. As the binding of PACAP to sulfated GAGs induced a random coil-to-α-helix conformational conversion, we investigated the role of the helical formation in PACAP internalization. Whereas this secondary structure was not crucial for efficient internalization in GAGs-deficient cells, PACAP α-helix was essential for GAGs-dependent uptake.. ...
MCH-1R antibody [C3], C-term (melanin-concentrating hormone receptor 1) for WB. Anti-MCH-1R pAb (GTX108185) is tested in Human samples. 100% Ab-Assurance.
PACAP (1-38), Human, Ovine, Rat, 10 mg. Kojro et al. observed that the PAC1 agonists PACAP-27 and PACAP-38 strongly increased the activity of ��-secretase.
PACAP (1-38), Human, Ovine, Rat, 5 mg. Kojro et al. observed that the PAC1 agonists PACAP-27 and PACAP-38 strongly increased the activity of ��-secretase.
Cellular models for the study of the neuropeptide melanin-concentrating hormone (MCH) have become indispensable tools for pharmacological profiling and signaling analysis of MCH and its synthetic analogues. Although expression of MCH receptors is most abundant in the brain, MCH-R(1) is also found in different peripheral tissues. Therefore, not only cell lines derived from nervous tissue but also from peripheral tissues that naturally express MCH receptors have been used to study receptor signaling and regulation. For screening of novel compounds, however, heterologous expression of MCH-R(1) or MCH-R(2) genes in HEK293, Chinese hamster ovary, COS-7, or 3T3-L1 cells, or amplified MCH-R(1) expression/signaling in IRM23 cells transfected with the G(q) protein gene are the preferred tools because of more distinct pharmacological effects induced by MCH, which include inhibition of cAMP formation, stimulation of inositol triphosphate production, increase in intracellular free Ca(2+) and/or activation ...
A fresh marine sediment certified reference material, NMIJ CRM 7306-a, for butyltin and phenyltin analysis has been prepared and certified by the National Metrological Institute of Japan at the National Institute of Advanced Industrial Science and Technology (NMIJ/AIST). of analytes in sample preparations. Tropolone was used as chelating agent in all the extraction methods. Certified values are given for TBT 443?g kg?1 as Sn, DBT 51 2?g kg?1 as Sn, MBT 67 625115-55-1 manufacture 3?g kg?1 as Sn, TPhT 6.9 1.2?g kg?1 as Sn, and DPhT 3.4 1.2?g kg?1 as Sn. These levels are less than in additional sediment CRMs designed for analysis of organotin chemical substances currently. option of NaBEt4 was ready inside a glove package that was purged with N2 gas. Additional chemicals used had been of analytical reagent quality. Pure water made by usage of a Milli-Q water-purification program (resistivity 18?M cm, Nihon Millipore Kogyo, Tokyo, Japan) was used through the entire experiments. Synthesis from the ...
One of the first studies to show convincing proof of agonist-specific states was transfection studies using the type-1 pituitary adenylyl cyclase-activating polypeptide (PACAP) receptor. The agonists (PACAP-27 and -38) stimulated adenylate cyclase (AC) with equal potencies, but only PACAP-38 could invoke the inositol phosphate response through phospholipase C (PLC) (Spengler et al., 1993). In subsequent work, the authors document the existence of a new splice variant of the PACAP receptor that was characterized by a 21-amino acid deletion in the N-terminal extracellular domain. They demonstrated that this domain modulates the receptor selectivity with respect to PACAP-27 and -38 binding and controls the relative potencies of the two agonists in phospholipase C stimulation (Pantaloni et al., 1996).. One of the first examples of agonist-specific states from mutational analysis was a Cys to Phe mutation in TM3 of the α1b-AR, a helix-turn below the critical Asp 125 involved in binding the ...
21 April 2017:. A new paper from CNBP researchers (lead author Wenjie Chen pictured) reports on the design of a new light-triggerable liposome. The work has just been published in the journal Molecular Therapy: Nucleic Acid and is accessible online.. Journal: Molecular Therapy: Nucleic Acid.. Title: Light-triggerable liposomes for enhanced endo/lysosomal escape and gene silencing in PC12 cells.. Authors: Wenjie Chen, Wei Deng, Ewa M. Goldys.. Abstract: Liposomes are an effective gene/drug delivery system, widely used in biomedical applications including gene therapy and chemotherapy. Here we designed a photo-responsive liposome (lipVP) loaded with a photosensitizer verteporfin (VP). This photosensitizer is clinically approved for photodynamic therapy (PDT). LipVP was employed as a DNA carrier for pituitary adenylyl cyclase-activating polypeptide (PACAP) receptor 1 (PAC1R) gene knockdown in PC12 cells. This has been done by incorporating PAC1R antisense oligonucleotides inside the lipVP cavity. ...
Melanokortin 1 receptor (MC1R), takođe poznat kao melanocit-stimulišući hormon receptor (MSHR), melanin-aktivirajući peptidni receptor, ili melanotropin receptor, jeste G protein-spregnuti receptor koji vezuje klasu pituitarni peptidnih hormona koji su poznati kao melanokortini.[1] U ovu grupu hormona se ubrajaju adrenokortikotropski hormon (ACTH) i različite forme melanocit-stimulišućih hormona (MSH). MC1R je jedan od ključnih proteina koji učestvuju u regulaciji sisarske boje kože i kose. On je lociran na membrani plazme specijalizovanih ćelija koje se zovu melanociti. Te ćelije proizvode pigment melanin procesom melanogeneze. Ovaj receptor kontroliše tip proizvedenog melanina. Aktivacija ovog receptor uzrokuje da melanociti pređu sa generisanja žutog ili crvenog feomelanina na smeđi ili crni eumelanin.. ...
Come cells may end up being handy magic size systems for medication finding and modelling human being illnesses while very well while to investigate cellular relationships and molecular occasions in the early phases of advancement. In basic principle, inbuilt elements such as cell features and extrinsic components launched by developing of come cells can result in growth development and immunological reactions after come cell transplantation. Restorative study displays there are many natural queries concerning security problems of come cell medical applications. Come cell therapy is definitely a quickly improving field that requires to concentrate even more on getting a extensive technology for evaluating risk. A range of risk elements (from inbuilt to extrinsic) should end up being regarded for secure scientific control cell therapies. farming of control cells which enhances the tumorigenicity risk (23,24). The primary factors behind the high risk for growth advancement by control cell therapy ...
PACAP receptor antibody (adenylate cyclase activating polypeptide 1 (pituitary) receptor type I) for IHC-P, WB. Anti-PACAP receptor pAb (GTX30026) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Fast delivery of MCHR1 knockout Human Cell Lines for the study of gene function. Created by CRISPR/Cas9 genome editing. Includes matched wildtype control.
TY - JOUR. T1 - Changes of dopamine content and cell proliferation by dexamethsone via pituitary adenylate cyclase-activating polypeptide in PC12 cell. AU - Yang, Ting Ting. AU - Tsao, Chiung Wen. AU - Li, Jin Shiou. AU - Wu, Hung Tsung. AU - Hsu, Chao Tien. AU - Cheng, Juei Tang. PY - 2007/10/9. Y1 - 2007/10/9. N2 - Pituitary adenylate cyclase-activating polypeptide (PACAP) is an endogenous neuropeptide observed in adrenal gland and sympathetic ganglia to regulate catecholamine synthesis and release. Both PACAP and glucocorticoid showed the activity to elevate catecholamine level through the stimulation of biosynthesis. However, the relationship of glucocorticoid and PACAP for this action is still unclear. Thus, alterations of gene expression, dopamine (DA) content, and cell proliferation in rat pheochromocytoma PC12 cells are employed as indicators to clarify this relationship in the present study. From the analysis of RT-PCR, the mRNA level of PACAP was observed to be raised by dexamethasone ...
Article Pituitary adenylate cyclase-activating polypeptide 38-mediated rin activation requires src and contributes to the regulation of hsp27 signaling during neuronal differentiation. Pituitary adenylate cyclase-activating polypeptide 38 (PACAP38) i...
TY - JOUR. T1 - The effect of pituitary adenylate cyclase-activating polypeptide on elevated plus maze behavior and hypothermia induced by morphine withdrawal. AU - Lipták, Nándor. AU - Dochnal, Roberta. AU - Babits, Anikó. AU - Csabafi, Krisztina. AU - Szakács, Júlia. AU - Tóth, Gábor. AU - Szabó, Gyula. PY - 2012/2/1. Y1 - 2012/2/1. N2 - The aim of the present investigation was to study the effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on morphine withdrawal-induced behavioral changes and hypothermia in male CFLP mice. Elevated plus maze (EPM) and jump tests were used to assess naloxone-precipitated morphine withdrawal-induced behavior responses. Different doses of subcutaneous (s.c.) naloxone, (0.1 and 0.2. mg/kg, respectively) were used to precipitate the emotional and psychical aspects of withdrawal on EPM and 1. mg/kg (s.c.) was used to induce the somatic withdrawal signs such as jumping, and the changes in body temperature. In our EPM studies, naloxone ...
Pituitary adenylate cyclase-activating polypeptide (PACAP) plays several important roles in vasodilation, neurotransmission, neuromodulation and neurotrophy, as well as activation of the trigeminovascular system. The aim of the present study was to explore the relationship between altered PACAP levels in peripheral blood and different types of headache. The present study enrolled 101 outpatients with headache and 35 healthy control volunteers. Blood samples were collected from the cubital vein and peripheral blood mononuclear cells (PBMCs) were separated. Total mRNA in the PBMCs was extracted and the expression of PACAP mRNA was analyzed by quantitative-polymerase chain reaction (Q-PCR). There was a significant decrease in PACAP mRNA expression in the PBMCs of the migraine (M) group relative to the healthy control group. However, there were no significant differences in PACAP mRNA expression between the control group and tension-type headache (TTH), cluster headache (CH), or medication overuse headache
Neurotrophic and neuroprotective effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on mesencephalic dopaminergic neurons ...
This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract (By similarity).
A multi-function neuropeptide that acts throughout the body by elevating intracellular cyclic AMP level via its interaction with PACAP RECEPTORS. Although first isolated from hypothalamic extracts and named for its action on the pituitary, it is widely distributed in the central and peripheral nervous systems. PACAP is important in the control of endocrine and homeostatic processes, such as secretion of pituitary and gut hormones and food intake ...
It has been observed that pituitary-adenylate cyclase activating polypeptide (PACAP) rescued DAergic neurons from neurodegeneration and improved motor alterations induced by 6-hydroxy-dopamine (6-OHDA) in rat parkinsonian models. Recently we investigated the molecular background of the neuroprotective effect of PACAP in DA-based neurodegeneration using rotenone-induced snail and 6-OHDA-induced rat models of Parkinsons disease. The behavioural activity, monoamine (DA and serotonin), metabolic enzyme (S-COMT, MB-COMT and MAO-B) and PARK7/DJ-1 protein contents were measured before and after PACAP-treatment in both models.. Locomotion and feeding activity were decreased in rotenone-treated snails which corresponded well to findings obtained in 6-OHDA- induced rat experiments. PACAP was able to prevent the behavioural malfunctions caused by the toxins. The monoamine levels decreased in both models and the decreased DA level induced by toxins was attenuated by ∼50% in the PACAP-treated animals. In ...
The pituitary adenylate cyclase-activating polypeptide (PACAP) is a peptide hormone, which binds to the pituitary adenylate cyclase type I (PAC1) receptor, and is recognized as a factor with pleiotropic physiological functions in many different organ systems. The PAC1R receptor is a G-protein coupled receptor and is part of the glucagon superfamily. The structure for the ectodomains of PAC1 receptor has been determined by nuclear magnetic resonance (NMR) and x-ray crystallography. However, the binding pose of PACAP remains debatable as the structure from NMR shows different topology from the structure derived from x-ray crystallography. In this study, we used molecular dynamic simulations to effectively understand the most stable and reliable interaction between this neuropeptide and its receptor. The binding pocket of PAC1 and interaction between PACAP and PAC1 receptor will be used to design PAC1 receptor peptidomimetics and small molecular antagonists.
Pituitary adenylate cyclase-activating polypeptide (PACAP) was originally isolated from the ovine brain in 1989 as a novel hypothalamic hormone that potently
TY - JOUR. T1 - γ-melanotropin and β-endorphin after dexamethasone. AU - Meador-Woodruff, James H.. AU - Silk, Kenneth R.. AU - Kronfol, Ziad. AU - Watson, Stanley J.. AU - Akil, Huda. PY - 1989/4/1. Y1 - 1989/4/1. UR - http://www.scopus.com/inward/record.url?scp=0024652543&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0024652543&partnerID=8YFLogxK. U2 - 10.1016/0006-3223(89)90278-3. DO - 10.1016/0006-3223(89)90278-3. M3 - Letter. C2 - 2541805. AN - SCOPUS:0024652543. VL - 25. SP - 975. EP - 976. JO - Biological Psychiatry. JF - Biological Psychiatry. SN - 0006-3223. IS - 7. ER - ...
Halaban, R and Lerner, A B., "The dual effect of melanocyte-stimulating hormone (msh) on the growth of cultured mouse melanoma cells." (1977). Subject Strain Bibliography 1977. 1167 ...
α-Melanocyte-Stimulating Hormone (MSH), amide stimulates melanocortin 1 receptor that results in the activation of adenylyl cyclase. - Mechanism of Action & Protocol.
References for Abcams α-MSH (α-Melanocyte-stimulating hormone) (ab120189). Please let us know if you have used this product in your publication
Undecylenoyl Phenylalanine Description:. Undecylenoyl Phenylalanine is a compound that includes amino acids and lipid residue, used in cosmetics and personal care products, specifically whitening formulas, to prevent skin cells from producing melanin pigmentation. Undecylenoyl Phenylalanine inhibits melanotropin, a melanin stimulator found in the skin. Melanotropin controls tyrosinase activity (tyrosinase being an essential enzyme in the production of melanin), melanin (eumalanin) synthesis and melanosome; therefore, by preventing the production of melanotropin, the pigmentation process is reduced or halted.. INCI Name: Undecylenoyl Phenylalanine. CAS No.: 175357-18-3. Solubility: Oil soluble. Appearance: White or off white powder. Typical Application: whitens , bleaching , lighten dark spots .. ...
PACAP is a pleiotropic neuropeptide that belongs to the secreting/glucagon/VIP family. PACAP functions as a hypothalamic hormone, neurotransmitter, neuromodulator, vasodilator, and neurotrophic factor. Its structure has been remarkably conserved during evolution. The PACAP receptor is G protein-coupled with seven transmembrane domains and also belongs to the VIP receptor family. PACAP, but not VIP, binds to PAC,SUB,1,/SUB,-R, whereas PACAP and VIP bind to VPAC,SUB,1,/SUB,-R and VPAC,SUB,2,/SUB,-R with a similar affinity. Despite the sizable homology of the structures of PACAP and VIP and their receptors, the distribution of these peptides and receptors is quite different. At least eight subtypes of PACAP specific, or PAC,SUB,1,/SUB,-R, result from alternate splicing. Each subtype is coupled with specific signaling pathways, and its expression is tissue or cell specific. Although PACAP fulfills most requirements for a physiological hypothalamic hypophysiotropic hormone, it does not consistently ...
メラニン凝集ホルモン(MCH)受容体の発見 : オーファン受容体ストラテジーが解決の鍵となる [in Japanese] Finding partner for orexigenic peptide : The receptor for melanin-concentrating hormone (MCH) is a G protein-coupled receptor [in Japanese] ...
Clinical Presentation The initial diagnosis of ACTH resistance may occur in infancy or later. In general, cases presenting in infancy will often have had a history of neonatal hypoglycaemia followed after several months by the observation that the child is excessively pigmented. Occasionally, pigmentation is commented on shortly after birth. Neonatal jaundice may also be an early feature indicative in these cases of glucocorticoid deficiency. Often, an excessively severe response to comparatively minor infective illnesses will result in the investigations that establish the diagnosis. Weber A, De Vroede M, Wienker TF, Jansen M: Clinical variability and molecular genetics in a family with triple A syndrome. Horm Res 1997;48(S2):191. Robbins LS, Nadeau JH, Johnson KR, Kelly MA, Roselli-Rehfuss L, Baack E, Mountjoy KG, Cone RD: Pigmentation phenotypes of variant extension locus alleles result from point mutations that alter MSH receptor function. Cell 1993;72:827-834. Light K, Jenkins PJ, Weber A, ...
Kabra DG, Pfuhlmann K, García-Cáceres C, Schriever SC, Casquero García V, Kebede AF, Fuente-Martin E, Trivedi C, Heppner K, Uhlenhaut NH, Legutko B, Kabra UD, Gao Y, Yi CX, Quarta C, Clemmensen C, Finan B, Müller TD, Meyer CW, Paez-Pereda M, Stemmer K, Woods SC, Perez-Tilve D, Schneider R, Olson EN, Tschöp MH, Pfluger PT. Hypothalamic leptin action is mediated by histone deacetylase 5. Nat Commun. 2016 Feb 29; 7:10782 ...
PACAP27 (Human) Antiserum Anti PACAP27 (Human) Serum Host Animal: RabbitY050 50 µl | 455.00 EUR Please ask for special prices for two ...
TY - JOUR. T1 - Pituitary adenylate cyclase-activating polypeptide prevents cytokine-induced cytotoxicity via inhibition of inducible nitric oxide synthase expression in βTC cells. AU - Sekiya, Kayo. AU - Nagasaki, Hiroshi. AU - Ozaki, Nobuaki. AU - Suzuki, Atsushi. AU - Miura, Yoshitaka. AU - Oiso, Yutaka. PY - 2000/11/11. Y1 - 2000/11/11. N2 - Type 1 diabetes mellitus is an autoimmune disease resulting from apoptotic destruction of pancreatic β-cells. The activation of inducible nitric oxide synthase (iNOS) by inflammatory cytokines is considered a mediator of destruction in β-cells. Recent findings showed that the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP), whose distribution was identified in pancreatic neurons, inhibited nitric oxide (NO) production in cytokine-activated macrophages. In the present study, we investigated the cytoprotective effect of PACAP in the cytokine-exposed mice β-cell line, βTC cells. 1 x 10 -8 M PACAP inhibited the reduction of cell ...
Looking for online definition of melanin-concentrating hormone in the Medical Dictionary? melanin-concentrating hormone explanation free. What is melanin-concentrating hormone? Meaning of melanin-concentrating hormone medical term. What does melanin-concentrating hormone mean?
Harmar AJ, Arimura A, Gozes I, Journot L, Laburthe M, Pisegna JR, Rawlings SR, Robberecht P, Said SI, Sreedaran SP, Wank SA, Waschek JA. 1998. International Union of Pharmacology. XVIII. Nomenclature of receptors for vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide. Pharmacol Rev 50:265-270 ...
Hypothalamic slices containing the lateral hypothalamic area (LHA) were prepared from 6- to 8-day-old rats and maintained in stationary culture for up to 35 days in order to analyse how well the melanin-concentrating hormone (MCH) neurons survived. As previously reported for other brain areas, this method yielded a long-term well-preserved organotypic organization. Light- and electron-microscopic investigations showed that differentiation continued and that synaptic contacts developed in vitro. After a period of elimination of damaged cells and fibres, most of the remaining neurons and glial cells retained a normal morphology throughout the culture period. MCH neurons, in particular, survived well as attested by the strong immunocytochemical and in situ hybridization signals still observed after several weeks. In a comparison with the day of explantation, competitive reverse transcription/polymerase chain reaction demonstrated the remarkable stability of the level of MCH mRNA at least until the 20th
Melanin-concentrating hormone (MCH) and orexin-A are orexigenic peptidergic neurotransmitters produced primarily in the lateral hypothalamus. Because two other hypothalamic peptides, neuropeptide Y and agouti-related peptide, increase food intake by a mechanism that depends on activation of opioid receptors, we assessed whether MCH or orexin-A also elicits food intake via opioid receptor activation. A dose of naloxone (0.3 mg/kg, ip) that had no effect on its own reduced the acute orexigenic effect of third ventricular (i3vt) orexin-A (3 ng/rat). However, this same dose of naloxone had no effect on i3vt MCH (5 microg/rat)-induced hyperphagia. Because the opioid system has also been linked to food selection, we investigated whether MCH or orexin-A alters food choice when rats have simultaneous access to two diets differing in the relative amounts of fat and carbohydrate. Whereas i3vt MCH stimulated intake of both diets and did not alter food choice, i3vt orexin-A stimulated intake of only the high fat
Affiliation:宮崎大学,医学部,助教, Research Field:Otorhinolaryngology,Otorhinolaryngology, Keywords:パッチクランプ,Endolymph,RT-PCR,Basigin,Endocochlear potential,Cochlea,Pituitary adenylate cyclase-activating polypeptide,In situ hybridization,血管条,分子生物学, # of Research Projects:4, # of Research Products:6
Adrenocorticotropin/α-melanocyte-stimulating hormone (ACTH/MSH)-like peptides modulate adenylate cyclase activity in rat brain slices: evidence for an ACTH/MSH receptor-coupled ...
Silva et al. (2009) published in the journal Nature that this same receptor, foxa2, is found in the hypothalamus (of mice) and it directly effects the hunger reflex. What they did was to take normal and genetically obese mice, fast them, and inject some of them with insulin to put them into the fed state. They then sacrificed the mice and dissected their brains, using an antibody-based stain to identify neurons that were positive for foxa2 and orexin and melanin-concentrating hormone (MCH). Orexin and MCH are known to be associated with feeding and, incidentally, sleep behaviour (Willie, 2001). From their results the authors concluded that the production of the neuropeptides orexin and melanin-concentrating hormone (MCH) were promoted by the foxa2 receptor (but only with insulin attached to it). One of the stronger pieces of evidence was that foxa2 was found in the cytoplasm of the mouse neurons when in the fasted state but in the nucleus when in the fed state. Transcription of messenger RNA ...
Melanin-concentrating hormone (MCH) is a cyclic orexinogenic hypothalamic peptide originally isolated from the pituitary gland of teleost fish where it controls skin pigmentation. In mammals it is involved in the regulation of feeding behavior and energy balance. When administered artificially, it increases food intake. It also seems to activate the stress axis. ...
Chemical-registry-number] 0 / ADCYAP1R1 protein, human; 0 / JV 1-53; 0 / RNA, Messenger; 0 / Receptors, Cell Surface; 0 / Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; 0 / Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; 0 / Receptors, Vasoactive Intestinal Peptide; 0 / Receptors, Vasoactive Intestinal Peptide, Type II; 0 / Receptors, Vasoactive Intestinal Polypeptide, Type I; 0 / VIPR1 protein, human; 0 / VIPR2 protein, human; 37221-79-7 / Vasoactive Intestinal Peptide; 9034-39-3 / Growth Hormone-Releasing ...
JNeurosci Print ISSN: 0270-6474 Online ISSN: 1529-2401. The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturers claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.. ...
In the goldfish pituitary, nerve fibers containing pituitary adenylate cyclase-activating polypeptide (PACAP) are located in close proximity to somatolactin (SL)-producing cells, and PACAP enhances SL release from cultured pituitary cells. However, there is little information about the mechanism of PACAP-induced SL release. In order to elucidate this issue, we used the cell immunoblot method. Treatment with PACAP at 10−8 and 10−7 M, but not with vasoactive intestinal polypeptide (VIP) at the same concentrations, increased the immunoblot area for SL-like immunoreactivity from dispersed pituitary cells, and PACAP-induced SL release was blocked by treatment with the PACAP selective receptor (PAC1R) antagonist, PACAP(6-38), at 10−6 M, but not with the PACAP/VIP receptor antagonist, VIP(6-28). PACAP-induced SL release was also attenuated by treatment with the calmodulin inhibitor, calmidazolium at 10−6 M. This led us to explore the signal transduction mechanism up to SL release, and we ...
Autonomic control of cardiac function depends on the coordinated activity generated by neurons within the intracardiac ganglia, and intrinsic feedback loops within the ganglia provide precise control of cardiac function. Both pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) are important regulators of cell-to-cell signaling within the intracardiac ganglia, and PACAP and VIP action on these ganglia, mediated through associated receptors, play an important role in the regulation of coronary blood flow, cardiac contraction, relaxation, and heart rate. Results reported here using PACAP and VIP provide direct evidence of some of the complex signaling which occurs in neurons of the rat intracardiac ganglia.
Breast cancer vasoactive intestinal peptide (VIP) receptors were characterized. Using in vitro autoradiographic techniques, 125I-labeled VIP bound with high affinity to breast biopsy sections. 125I-labeled VIP bound specifically to five breast cancer cell lines examined using receptor-binding techniques. Specific 125I-labeled VIP binding to MDA-MB-231 cells was inhibited with high affinity by VIP and pituitary adenylate cyclase-activating polypeptide (ICb50 = 2 nm) and with moderate affinity by the VIP hybrid (IC50 = 0.5 µm) VIP elevated the cAMP in a dose-dependent manner, and VIP hybrid (10 µm) inhibited the increase in cAMP caused by VIP. Using Northern blot analysis, VIP (10 nm) stimulated c-fos and c-myc mRNA, and the increase caused by VIP was reversed by the VIP hybrid. The VIP hybrid inhibited breast cancer growth in vitro and in vivo using nude mice bearing breast cancer xenografts. These data suggest that the VIP hybrid is a breast cancer VIP receptor antagonist.. ...
TY - JOUR. T1 - Changes in expression of neuropeptides and their receptors in the hypothalamus and gastrointestinal tract of calorie restricted hens. AU - Simon, Ádám. AU - Oláh, János. AU - Komlósi, István. AU - Jávor, András. AU - Németh, József. AU - Szilvássy, Zoltán. AU - Reglodi, Dóra. AU - Tamás, Andrea. AU - Czeglédi, Levente. PY - 2017/9. Y1 - 2017/9. N2 - The list of orexigenic and anorexigenic peptides, those are known to alter feed intake, is continuously growing. However, most of them are studied in mammalian species. We aimed to investigate plasma level and mRNA expression of the pituitary adenylate cyclase-activating polypeptide (PACAP), gene expression of its receptor (PAC1), furthermore the gene expression of galanin (GAL), neuromedin U (NMU), and its two receptors (NMUR1 and NMUR2) in the hypothalamus, proventriculus, and jejunum of hens exposed to 40% calorie restriction. Feed restriction resulted in a 88% increase in mRNA and a 27% increase in peptide level of ...
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