Our findings have significant implications for theories regarding multistage carcinogenesis, as well as for the diagnosis, treatment, and prevention of cancer. Our data suggest that EPO and EPOR expression may contribute to the survival of hypoxic solid tumors. Hypoxia is known to select for aggressive cancer phenotypes and to promote tumor neovascularization (12) . Our finding of high EPO and EPOR levels in human solid tumors, as well as our demonstration of hypoxic up-regulation of both of these proteins, suggests novel ways by which hypoxia may contribute to cancer promotion. EPO and EPOR proteins are up-regulated after ischemic stroke in the rat brain (16) and EPO enhances ischemic and hypoxic brain cell survival (16 , 17) . The high expression of EPO and EPOR in solid tumors could similarly improve the hypoxic or ischemic survival of cancer cells. Hypoxia induces transcription of the EPO gene via activation of the transcription factor HIF-1 (1 , 11 , 12) . Overexpression of HIF-1 is common ...
The expression in cancer cells of EpoR-the mediator of the biological effects of Epo in erythroid cells-has been examined by many laboratories using a variety of techniques including reverse transcription-PCR to show EpoR mRNA transcripts, and immunoblotting, immunoprecipitation, or immunohistochemistry studies to investigate EpoR protein expression (2). In some studies, expression of cell surface EpoR has been examined in cultured cancer cell lines using radiolabeled Epo binding or a novel receptor-mediated endocytosis assay (5), reporting either the absence of specific radioligand binding (6) or the presence of significantly fewer frequency of cell surface receptors compared with erythroid cells that are nevertheless sufficient to mediate proliferative or antiapoptotic effects in some cell lines (5, 7, 8). Thus, a correlation between the level of tumor cell EpoR expression and the responsiveness to exogenous Epo to induce functional intracellular signal transduction in cancer cells has not ...
Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate LYN tyrosine kinase (By similarity).
STATegics has discovered small molecules that selectively activate the tissue-protective erythropoietin (EPO) receptor, but not the EPO receptor that plays a role in red blood cell production. In previous studies, the lead compound demonstrated potent neuroprotective effects, favorable safety profile and efficacy in protecting dopaminergic neurons in models of Parkinson s disease when given by injections. The compound has the potential to slow down the progression of Parkinson s disease and to repair some of the damaged brain tissue.. Hypothesis ...
Summary of Invention: The present invention relates to the generation of a novel population of Epo-responsive marrow derived cells that express Epo-responsive genes and gene products. Said novel population of Epo-responsive marrow derived cells are generated by isolating a subset of bone marrow cells that are, in one embodiment, Kit.sup.posCD71.sup.high, then culturing the Kit.sup.posCD71.sup.high cells in the absence of Epo or in the absence of hematopoietic cytokines (including Epo) for a period of time sufficient to substantially decrease Epo receptor signaling and then exposing them to Epo for a period of time sufficient to permit the expression of Epo-responsive genes. The present invention also relates to the detection of Epo-responsive genes and gene products. Further, the present invention relates to the detection of the administration of Epo, Epo-derivatives and Epo-mimetics in subjects based on the detection of expression of Epo-responsive genes.. Advantages:. Patent #: ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Introduction: The role of erythropoietin (Epo) in myocardial repair after infarction remains inconclusive. We observed high Epo receptor (EPOR) expression in cardiac progenitor cells (CPCs). Therefore, we aimed to characterize these cells and elucida
Erythropoietin, the cytokine required for erythrocyte production, contributes to muscle progenitor cell proliferation and survival in culture and animal models....
Citation: N/A Interpretive Summary: Technical Abstract: Previous studies have indicated a fetal loss of approximately 30% in pigs between d 25 and d 50 of pregnancy when fetuses are in a crowded uterine environment. A rapid expansion of the embryonic/fetal blood supply also occurs at this time and may be an important factor in fetal survivability and litter size in pigs. To understand this process, we have cloned a partial cDNA for the porcine (p) erythropoietin receptor (EPOR) and examined the expression of pEPOR mRNA in embryonic/fetal liver on gestational d 24, 30 and 40. Using total RNA from d 30 embryonic pig liver, reverse transcription (RT) followed by polymerase chain reaction (PCR) with primers generated using an EPOR consensus sequence, a partial cDNA of 532 bp (corresponding to a portion of the extracellular domain) for pEPOR was subcloned and sequenced. Additional RT-PCR generated a 867 bp fragment (including most of the cytoplasmic domain). Rapid amplification of cDNA ends (RACE) ...
Given these concerns, the mechanisms of action of ESAs, particularly their effects on nonerythroid cells, have recently come under investigation. Many common tumor types, including lung ( 12), squamous head and neck ( 13), breast ( 14), colon ( 15), gastric ( 16), and uterine ( 17) cancers, have been shown to express Epo receptor (EpoR). EpoR is classified as a type 1 cytokine receptor with a single transmembrane domain with three identifiable forms: full length, truncated, and soluble. Its exact physiologic roles in nonerythroid cell lines remain unclear. It is hypothesized that Epo and EpoR are synthesized by tumor cells and stimulation in an autocrine/paracrine fashion affects cancer cell growth and survival ( 12, 18- 20). With prominent expression of EpoR on tumor cell surfaces, endogenous production of Epo can result in downstream signaling cascades. Signaling pathways activated by EpoR stimulation result in increased migration ( 20), invasion ( 13, 18), proliferation ( 21), angiogenesis ( ...
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Erythropoietin is an essential growth factor that promotes survival, proliferation, and differentiation of mammalian erythroid progenitor cells. Erythropoietin(−/−) and erythropoietin receptor(−/−) mouse embryos die around embryonic day 13.5 due, in part, to failure of erythropoiesis in the fetal liver. In this study, we demonstrated a novel role of erythropoietin and erythropoietin receptor in cardiac development in vivo. We found that erythropoietin receptor is expressed in the developing murine heart in a temporal and cell type-specific manner: it is initially detected by embryonic day 10.5 and persists until day 14.5. Both erythropoietin(−/−) and erythropoietin receptor(−/−) embryos suffered from ventricular hypoplasia at day 12-13 of gestation. This defect appears to be independent from the general state of hypoxia and is likely due to a reduction in the number of proliferating cardiac myocytes in the ventricular myocardium. Cell proliferation assays revealed that ...
Background Our original demonstration of immunomodulatory effects of erythropoietin in multiple myeloma, led us to the search of the cells in the immune system that are direct targets to erythropoietin. The finding that lymphocytes do not express erythropoietin receptors, has led to the hypothesis that other cells act as direct targets and thus mediate the erythropoietin effects. Having found erythropoietin effects on dendritic cells thus led to the question of whether macrophages act as target cells to erythropoietin. Design and Methods EPO effects on macrophages were investigated both in-vivo and in-vitro. The in-vivo studies were performed on splenic macrophages and inflammatory peritoneal macrophages, in recombinant human erythropoietin -treated, compared to untreated mice, as well as in transgenic mice over-expressing human erythropoietin (tg6), compared to their control wild type counterparts. The in-vitro effects of erythropoietin on macrophage surface markers and function were ...
1. Wu H, Liu X, Jaenisch R, Lodish HF. Generation of committed erythroid BFU-E and CFU-E progenitors does not require erythropoietin or the erythropoietin receptor. Cell. 1995;83:59-67 2. Miyake T, Kung CK, Goldwasser E. Purification of human erythropoietin. The Journal of biological chemistry. 1977;252:5558-64 3. Lin FK, Suggs S, Lin CH, Browne JK, Smalling R, Egrie JC. et al. Cloning and expression of the human erythropoietin gene. Proceedings of the National Academy of Sciences of the United States of America. 1985;82:7580-4 4. Jacobs K, Shoemaker C, Rudersdorf R, Neill SD, Kaufman RJ, Mufson A. et al. Isolation and characterization of genomic and cDNA clones of human erythropoietin. Nature. 1985;313:806-10 5. Noguchi CT, Wang L, Rogers HM, Teng R, Jia Y. Survival and proliferative roles of erythropoietin beyond the erythroid lineage. Expert reviews in molecular medicine. 2008;10:e36. doi:10.1017/S1462399408000860 6. Teng R, Gavrilova O, Suzuki N, Chanturiya T, Schimel D, Hugendubler L. et ...
Purpose: Erythropoiesis-stimulating agents (ESAs) are used clinically for treating cancer-related anemia. Recent clinical trials have reported increased adverse events and reduced survival in ESA-treated breast cancer patients receiving chemotherapy, potentially related to erythropoietin (EPO)-induced cancer progression. However, minimal pre-clinical data is available regarding the impact of EPO on metastatic cell behavior and/or the metastatic process, and this was the goal of our study. Experimental Design: Breast cancer cell lines were treated with recombinant human EPO (rHuEPO) and screened for expression of EPOR. MDA-MB-231 and MDA-MB-435 cell lines were used for functional assays in vitro (2D/3D growth, survival) and in vivo (tumorigenicity, metastasis), in the presence or absence of EPO and/or cytotoxic agents. Results: A large variation in EPOR expression across cell lines was observed. In vitro, rHuEPO had a protective effect on radiation-treated MDA-MB-435 cells (p,0.05), however ...
Lung cancer, with its most prevalent form non-small-cell lung carcinoma (NSCLC), is one of the leading causes of cancer-related deaths worldwide, and is commonly treated with chemotherapeutic drugs … such as cisplatin. Lung cancer patients frequently suffer from chemotherapy-induced anemia, which can be treated with erythropoietin (EPO). However, studies have indicated that EPO not only promotes erythropoiesis in hematopoietic cells, but may also enhance survival of NSCLC cells. Here, we verified that the NSCLC cell line H838 expresses functional erythropoietin receptors (EPOR) and that treatment with EPO reduces cisplatin-induced apoptosis. To pinpoint differences in EPO-induced survival signaling in erythroid progenitor cells (CFU-E, colony forming unit-erythroid) and H838 cells, we combined mathematical modeling with a method for feature selection, the L1 regularization. Utilizing an example model and simulated data, we demonstrated that this approach enables the accurate identification and ...
Title: Erythropoietin: New Approaches to Improved Molecular Designs and Therapeutic Alternatives. VOLUME: 14 ISSUE: 13. Author(s):N. Debeljak and A. J. Sytkowski. Affiliation:Laboratory for Cell and Molecular Biology, Division of Hematology and Oncology, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School,330 Brookline Ave., W/BL 548, Boston, MA 02215, USA.. Keywords:Erythropoietin, erythropoietin receptor, glycosylation, pegylation, fusion proteins, mimetics, heteroreceptor, cancer. Abstract: Erythropoietin (Epo) is a glycoprotein hormone that is the prime regulator of erythropoiesis. Recombinant Epo is a highly effective pharmaceutical used to correct anemias associated with renal insufficiency, cancer and other diseases. Efforts to increase its efficacy in vivo by manipulating the proteins structure have met with some success, and novel Epo-like agents are in development. Additionally, efforts to create Epo mimetic agents are underway, as is the design of ...
Erythropoietin is a substance produced by the kidney that leads to the formation of red blood cells in the bone marrow. It is a glycoprotein hormone which regulates erythropoiesis (Red Blood Cell production). For erythrocyte precursors present in the bone marrow, EPO acts as a cytokine. Commonly referred to as hematopoietin or hemopoietin, erythropoietin is also known to have other biological functions, such as involvement in the wound healing cycle and brains response to neuronal injury. People suffering from End Stage Renal Disease (ESRD), HIV or undergoing chemotherapy are not able to produce enough EPO on their own and thus, are administered with synthetic or recombinant erythropoietin which has the similar sequence of amino acids. Recombinant erythropoietin is a kind of therapeutic agent devised using DNA technology.. Request a sample of this report at http://www.orbisresearch.com/contacts/request-sample/127811 .. On the bases of their molecular structure, EPO drugs can be divided into ...
The membrane-assisted isoform immunoassay (MAIIA) quantitates erythropoietin (EPO) isoforms as percentages of migrated isoforms (PMI). We evaluated the effect of recombinant human EPO (rhEPO) on the distribution of EPO isoforms in plasma in a randomized, placebo-controlled, double-blinded, cross-over study. 16 healthy subjects received either low-dose Epoetin beta (5000 IU on days 1, 3, 5, 7, 9, 11 and 13); high-dose Epoetin beta (30.000 IU on days 1, 2 and 3 and placebo on days 5, 7, 9, 11 and 13); or placebo on all days. PMI on days 4, 11 and 25 was determined by interaction of N-acetyl glucosamine with the glycosylation dependent desorption of EPO isoforms. At day 25, plasma-EPO in both rhEPO groups had returned to values not different from the placebo group. PMI with placebo, reflecting the endogenous EPO isoforms, averaged 82.5 (10.3) % (mean (SD)). High-dose Epoetin beta decreased PMI on days 4 and 11 to 31.0 (4.2)% (p,0.00001) and 45.2 (7.3)% (p,0.00001). Low-dose Epoetin beta decreased ...
The expression system was used to create recombinant human erythropoietin, a protein synthesized by the adult kidney and responsible for the regulation of red blood cell production. of recombinant EPO and increase the activity of TG-101348 this protein Yeasts have long been a model organism for biochemical and genetic studies because of the advantages they offer compared to bacterial systems, including the ease with which they can cultured and managed, and the fact that they share several important biological characteristics with eukaryotic cells, such as splicing and other processes involved in post-translational modifications. Several yeast species have been used to generate recombinant proteins, including and (examined in B?er offers similar advantages to other yeasts, and are preferred as the overall length of the mannose outer chains is shorter than in (Kang and construction of the gene The entire human erythropoietin gene was constructed using the Splicing by Overlap-Extension by PCR ...
Erythropoietin, EPO, is the main regulator and stimulator of bone marrow erythropoiesis, and is responsible for growth and differentiation of the erythr
Erythropoietin (EPO) regulates proliferation and differentiation of erythroid precursor cells into erythrocytes. The last decade has revealed non-renal sites of EPO production and extrahematopoietic expression of the EPO-receptor (EPO-R), thus suggesting that EPO has pleiotropic functions. Here we addressed the interplay between EPO / glucose metabolism / body weight, by employing a panel of relevant experimental murine models. The models focused on situations of increased EPO levels, including EPO injected C57BL/6 and BALB/c mice, as well as transgenic mice (tg6) constitutively overexpressing human EPO, thus exposed to constantly high EPO serum levels. As experimental models for diabetes and obesity, we employed protein Tyr phosphatase 1B (PTP1B) knockout mice associated with resistance to diabetes (PTP1B-/-), and ob/ob mice susceptible to diabetes and obesity. The data presented herein demonstrate EPO-mediated decrease in blood glucose levels in all mice models tested. Moreover, in the ob/ob ...
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Symptoms of Erythropoietin-induced hypertension including 22 medical symptoms and signs of Erythropoietin-induced hypertension, alternative diagnoses, misdiagnosis, and correct diagnosis for Erythropoietin-induced hypertension signs or Erythropoietin-induced hypertension symptoms.
20:57, 15 May 2016 (diff , hist) (+574)‎ N File:The process of erythropoiesis.jpg ‎ (The process of erythropoiesis. Erythroid progenitors in the bone marrow that depend on Epo and EpoR for differentiation into mature red blood cells (a). The signaling pathways stimulated by EpoR upon binding to Epo (b). EpoR erythropoietin receptor, Ep...) ...
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METHODS: Thirty-five rats were divided into 3 groups. In the baseline control group (BC, n=7), rats were uninjured and untreated. In the positive control group (PC, n=21) rats were injured but untreated. In the EPO-24 group (n=7), rats were injured and a single dose of intra-peritoneal EPO (5000 IU/kg) was administered immediately after lung injury. The PC group was divided into 3 subgroups: PC-6 (n=7), PC-12 (n=7), and PC-24 (n=7). The BC group was subjected to thoracotomy, and the right lung was harvested. The PC subgroups were eu-thanized at 6, 12, and 24 hours after injury, respectively. The EPO-24 group was euthanized at the 24th hour after injury. Lung samples were obtained, levels of malondialdehyde (MDA) and EPO were analyzed, and activities of superoxide dismutase (SOD) and catalase (CAT) were then measured in homogenized lung tissue samples. Histologic damage to lung tissue in the BC group, the EPO-24 group, and PC subgroup euthanized at the 24th hour after injury were scored by a ...
Abcams Erythropoietin (EPO) ELISA Kit (ab119522) suitable for Cell culture supernatant, Serum, Plasma in human. Reliably quantify 0.4 mIU/ml of Erythropoietin…
Suppressor of cytokine signaling 2 is a protein that in humans is encoded by the SOCS2 gene. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signalling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10 and interferon-gamma (IFN-gamma). The protein encoded by this gene is found to interact with the cytoplasmic domain of insulin-like growth factor 1 receptor (IGF1R), and thus is thought to be involved in the regulation of IGF1R mediated cell signaling. Knockout studies in mice also suggested a regulatory role of this gene in IGF-1 related growth control. SOCS2 has been shown to interact with insulin-like growth factor 1 receptor and erythropoietin receptor. GRCh38: Ensembl release 89: ENSG00000120833 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000020027 - Ensembl, May ...
The identification of amino acid residues essential for function of the hematopoietic growth factor erythropoietin has been approached by several methods, including comparisons of related sequences, immunochemical approaches, mutagenesis and computer modeling. We have reported previously that mutations within amino acids 100-109 of erythropoietin can have profound effects on the hormones structure and/or activity and that Arg103 is especially important for function (Chern, Y., Chung, T. & Sytkowski, A.J. (1991) Eur. J: Biochem. 202, 225-229; Grodberg, J. Davis, K. L. & Sytkowski, A. J. (1993) Eur. J Biochem. (218, 597-601). We have now constructed a series of Arg 103 substitutions in order to determine the structural features of amino acid 103 required for biological activity. Each of the mutants was expressed and secreted efficiently by transfected COS1 cells. Mutants Arg103Asn, Arg103Gln, Arg103CGlu exhibited no biological activity. In contrast, Arg103His and Arg103Lys had specific activities equal
Cytokine-inducible SH2-containing protein is a protein that in humans is encoded by the CISH gene. CISH orthologs have been identified in most mammals with sequenced genomes. CISH controls T cell receptor (TCR) signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL-2, IL-3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. CISH is induced by T cell receptor (TCR) ligation and negatively regulates it by targeting ...
Cellular and molecular mechanisms regulating the hepatic erythropoietin expression during acute-phase response: a role for IL-6 Ramadori P, Ahmad G, Ramadori G. Source Department of Gastroenterology and Endocrinology, Center of Internal Medicine, University of Göttingen, Göttingen, Germany. Abstract The source of circulating erythropoietin (EPO), the mediators and the mechanisms involved in the upregulation of EPO…
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EPO, Erythropoietin, is an acidic glycoprotein hormone with a molecular mass of 34 kD. It is a cytokine for erythrocyte (red blood cell) precursors in…
Weve given her the first erythropoietin jab. Have to wait for 3-5 days for the body to respond. Praying hard this will help ...
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buy epo,buy epo online,where to buy epo,epo buy,epo injections,epo injection,Erythropoietin or EPO is one of the latest synthetic hormones that are used by athletes so they can play better in their respective sports. EPO is basically a protein hormone from your kidney and liver and it is supposed to bind with your bone marrow receptors after being released into your bloodstream. This can stimulate the production of erythrocytes or red blood cells.
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TY - JOUR. T1 - A friend virus mutant that overcomes Fv-2rr host resistance encodes a small glycoprotein that dimerizes, is processed to cell surfaces, and specifically activates erythropoietin receptors. AU - Kozak, Susan L.. AU - Hoatlin, Maureen E.. AU - Ferro, Frank E.. AU - Majumdar, Manas K.. AU - Geib, Roy W.. AU - Fox, Mary T.. AU - Kabat, David. PY - 1993/5. Y1 - 1993/5. N2 - The env gene of Friend spleen focus-forming virus (SFFV) encodes a membrane glycoprotein (gp55) that is inefficiently (3 to 5%) processed from the rough endoplasmic reticulum to form a larger dimeric plasma membrane derivative (gp55p). Moreover, the SFFV env glycoprotein associates with erythropoietin receptors (EpoR) to cause proliferation of infected erythroblasts [J.-P. Li, A. D. DAndrea, H. F. Lodish, and D. Baltimore, Nature (London) 343:762-764, 1990]. Interestingly, the mitogenic effect of SFFV is blocked in mice homozygous for the Fv-2r resistance gene, but mutant SFFVs can overcome this resistance. Recent ...
... , or its alternativeerythropoetin orEPO, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Also calledhematopoietin orhemopoietin, it is produced by the peritubular capillary endothelial cells in the kidney, and is the hormone that regulates red blood cell production. It also has other known biological functions. For example, erythropoietin plays an important role in the brain`s response to neuronal injury. EPO is also involved in the wound healing process. When exogenous EPO is used as a performance-enhancing drug, it is classified as an erythropoiesis-stimulating agent (ESA). Exogenous EPO can often be detected in blood, due to slight difference from the endogenous protein, for example in features of posttranslational modification. History In 1906, Paul Carnot, a professor of medicine in Paris, and his assistant DeFlandre proposed the idea that hormones regulate ...
Synonyms: Familial erythrocytosis, Primary congenital erythrocytosis, Familial erythrocytosis type 1, ECYT1, Polycythemia, primary familial and congenital, PFCP, Erythrocytosis autosomal dominant benign, Congenital polycythemia due to erythropoietin receptor mutation, Congenital erythrocytosis due to erythropoietin receptor mutation, Autosomal dominant familial erythrocytosis-1, Familial erythrocytosis 1, Primary familial polycythemia ...
We recently determined that erythropoietin (EPO) activates 3 members of the signal transducer and activator of transcription (STAT) family, Stat1α, Stat3, and Stat5, in the human EPO-dependent cell lines, UT-7 and UT-7/EPO (Kirito et al, J Biol Chem 272:16507, 1997). In addition, we have shown that Stat1α, but not Stat3, is involved in EPO-induced cellular proliferation. In this study, we examined the roles of Stat1α and Stat3 in EPO-induced erythroid differentiation. UT-7/GM was used as a model system, because this cell line can differentiate into erythroid-lineage cells with EPO treatment (Komatsu et al, Blood 89:4021, 1997). We found that EPO did not activate Stat1α or Stat3 in UT-7/GM cells. Transfection experiments showed that both Stat1α and Stat3 inhibited the induction by EPO of γ-globin and erythroid-specific 5-aminolevulinate synthetase transcripts, resulting in a reduction of the percentage of hemoglobin-positive cells. Dominant negative forms of Stat1α or Stat3 promoted the ...
TY - JOUR. T1 - Erythropoietin administration protects retinal neurons from acute ischemia-reperfusion injury. AU - Junk, Anna. AU - Mammis, Antonios. AU - Savitz, Sean I.. AU - Singh, Manjeet. AU - Roth, Steven. AU - Malhotra, Samit. AU - Rosenbaum, Pearl S.. AU - Cerami, Anthony. AU - Brines, Michael. AU - Rosenbaum, Daniel M.. PY - 2002/8/1. Y1 - 2002/8/1. N2 - Erythropoietin (EPO) plays an important role in the brains response to neuronal injury. Systemic administration of recombinant human EPO (rhEPO) protects neurons from injury after middle cerebral artery occlusion, traumatic brain injury, neuroinflammation, and excitotoxicity. Protection is in part mediated by antiapoptotic mechanisms. We conducted parallel studies of rhEPO in a model of transient global retinal ischemia induced by raising intraocular pressure, which is a clinically relevant model for retinal diseases. We observed abundant expression of EPO receptor (EPO-R) throughout the ischemic retina. Neutralization of endogenous ...
TY - JOUR. T1 - Polarized THG Microscopy Identifies Compositionally Different Lipid Droplets in Mammalian Cells. AU - Bautista, Godofredo. AU - Pfisterer, Simon G.. AU - Huttunen, Mikko J.. AU - Ranjan, Sanjeev. AU - Kanerva, Kristiina. AU - Ikonen, Elina. AU - Kauranen, Martti. PY - 2014/11/18. Y1 - 2014/11/18. KW - MOLECULAR-STRUCTURE DETERMINATION. KW - INTEGRAL MEMBRANE-PROTEINS. KW - ERYTHROPOIETIN RECEPTOR. KW - TRANSMEMBRANE DOMAIN. KW - SIGNAL-TRANSDUCTION. KW - NMR-SPECTROSCOPY. KW - XPLOR-NIH. KW - DIACYLGLYCEROL KINASE. KW - DETERGENT MICELLES. KW - ACTIVATION. KW - 3111 Biomedicine. U2 - 10.1016/j.bpj.2014.10.009. DO - 10.1016/j.bpj.2014.10.009. M3 - Article. VL - 107. SP - 2230. EP - 2336. JO - Biophysical Journal. JF - Biophysical Journal. SN - 0006-3495. IS - 10. ER - ...
A more serious side effect of Erythropoietin may be high blood pressure. This can happen if red blood cells are produced too rapidly. Your doctor may need to reduce or withhold your dose of Erythropoietin and initiate or increase blood pressure medication ...