Hill, D J. and Borysenko, J Z., Restriction of patching of bound concanavalin a after incorporation of arachidonic acid into the plasma membrane of virally transformed fibroblasts. (1979). Subject Strain Bibliography 1979. 3532 ...

As far as we know, our experiments are the first to combine single-molecule imaging and tracking with simultaneous whole-cell voltage-clamp recordings to measure receptor mobility and function concomitantly. We found that receptor lateral mobility doubled when the channels were opened and that this required calcium flux. The evidence to suggest this is threefold. First, the ATP-evoked increase in mobility was blocked by recording in calcium-free extracellular buffers. Second, S340Y mutants that are known to abrogate calcium flux also lacked ATP-evoked increases in P2X2 mobility (Migita et al., 2001; Egan and Khakh, 2004). Third, ATP-evoked mobility increases were blocked by voltage clamping the cells at positive voltages (+70 mV), when the inward driving force on calcium ions was substantially reduced. However, ATP-evoked P2X2 receptor mobility increases were not blocked by intracellular dialysis with BAPTA, implying that calcium entry via P2X2 receptors acts locally to increase mobility. ...

The issue of whether the difference in mobile fraction between the anterior and the posterior of gradient-sensing D. discoideum cells was caused by a conformational change of the receptor owing to its phosphorylation state, or by an altered interaction between the cAR1 receptor and its associated G-protein, was addressed by comparative studies with mutant cell lines. We analyzed a phosphorylation deficient mutant expressing a cAR1 receptor in which the four serine clusters in the C-terminal tail were substituted (cm1234-eYFP) (Kim et al., 1997). cm1234-eYFP/car1- cells were found to be defective in propagation of the cAMP wave (Kim et al., 1997), leading to very small aggregation centres during development. Therefore, cells expressing cm1234-eYFP were measured very close to the aggregation centre, a situation reflecting that of the needle assay. For cm1234-eYFP, the fraction of mobile receptors at the anterior exceeded that at the posterior by a factor of 1.7 (72% versus 43%, see supplementary ...

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Gentaur molecular products has all kinds of products like :search , Biotium \ Concanavalin A, CF350 conjugate \ 29015 for more molecular products just contact us

MAMFKTLSFL FLLCFQIHGT VAADENSLFS FNGFVKSPSF DKRVALFGDS KLVNDSSLIQ LTDSVTRSVG RVVYKKPINL FKGKQRNSRS FSTHFSFSSM SNEIGDDVLA FVMVPSSLDL SLFGKKDNSS SALGFLSQYA KNETVVAVEF GISRTGNSAR ILVGRPESAE IRNLSFVGDL MMDNGRTLSC MIEYEASSKR MMVRFRKSGS VKLFDPFFSF SVDLAKLWKG GEVTVGLSSA NGNSSSKAHF LHSWRFEIRH PPPMWMHSVP LEPNEVSKEE ENGRGRSECT RKMLGALIFG AACGALGALL GLYLWTICRV RRSMAVVPEE CAIEQGKK ...

She does not appear to be losing weight, at least not so far. Sometimes relief from the pain of built up stomach gas can be had by belching, having a bowel movement or breaking wind. My cat is 18 years old and her stomach is making he most strange sounds, gurgling and rumbling continuously. Increased production of acid at the level of the stomach. Gas can only be relieved at the top and the bottom of the digestive tract, through burping or flatulence. It makes us feel embarrassed and at times the cramps and bloating can be painful too. Gas is one of the most typical causes. I have rumbling and gurgling in my stomach, slight nausea at times and diarrhea. Not only is gas, bloating and rumbling intestines an uncomfortable condition, it could be more of a concern if one is in public when the problem arises. Cramps, noise in the stomach and other symptoms are due to premenstrual syndrome. Chances are you barely know what your gallbladder is let alone which gallbladder attack symptoms to look out ...

Researchers in Bordeaux recently discovered a new mechanism for storing information in synapses and a means of controlling the storage process. The breakthrough moves science closer to unveiling the mystery of the molecular mechanisms of memory and learning processes. The research, carried out primarily by researchers at the Interdisciplinary Institute for Neurosciences (CNRS/Université de Bordeaux) and the Bordeaux Imaging Center (CNRS/Université de Bordeaux/Inserm), appears in the 13 september 2017 edition of Nature.

The flow of information through the epidermal growth factor receptor (EGFR) is shaped by molecular interactions in the plasma membrane. The EGFR is associated with lipid rafts, but their role in modulating receptor mobility and subsequent interactions is unclear. To investigate the role of nanoscale …

A flow cytometry assay that can be used to directly determine the proportion of activated T lymphocytes in human whole blood samples after stimulation with concanavalin A is presented here

TY - JOUR. T1 - Uniform distribution of two-term recurrence sequences. AU - Vélez, William Yslas. PY - 1987/5. Y1 - 1987/5. N2 - Let (FORMULA PRESENTED), A, B be rational integers and for (FORMULA PRESENTED). The sequence (un) is clearly periodic modulo m and we say that (un) is uniformly distributed modulo m if for every 5, every residue modulo m occurs the same number of times in the sequence of residues (FORMULA PRESENTED), where N is the period of (un) modulo m. If (un) is uniformly distributed modulo m then m divides N, so we write N=mf. Several authors have characterized those m for which (un) is uniformly distributed modulo m. In fact in this paper we will show that a much stronger property holds when m = pk, p a prime. Namely, if (un) is uniformly distributed modulo pk with period pkf, then every residue modulo pk appears exactly once in the sequence (FORMULA PRESENTED), for every s. We also characterize those composite m for which this more stringent property holds.. AB - Let (FORMULA ...

Im just going to throw this one out there verbatim. A contractor has found through experience that the low bid for a job (excluding his own bid) is a

Ever heard of dry stone walling? Its worth investigating if youre looking for a ruggedly beautiful, green type of fence that stands the test of time. Read our interview with John Bland of Montreals John Bland Stonecraft and discover the ancient craft of dry stone walling. What is a dry stone wall? A dry stone wall is built with only one ingredient - natural stone. Theres no mortar gluing everything together or backup made of concrete blocks. How does it stay up? You

Chye, ML (2010). Arabidopsis acyl-Coenzyme A-binding proteins. In AOCS Lipid Library, Plant Lipid Biochemistry Section. http://lipidlibrary.aocs.org/Biochemistry/content.cfm?ItemNumber=40301 ...

Summary A variety of cells infected with different enveloped RNA viruses are specifically agglutinated by Concanavalin A. The production of envelope-components seems to be essential for the agglutinability of cells infected with myxovirus. A strong flocculation of purified virus particles by Concanavalin A indicates that the specific receptor of the host cell membrane is incorporated into the virus envelope.

Mechanism of human lymphocyte stimulation by concanavalin A: role of valence and surface binding sites.: A monovalent form of concanavalin A (m-Con A) has been

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Bangalore-based software-as-a-service (SaaS) provider Capillary Technologies Pvt. Ltd has roped in former Yum! Brands executive Sunil Suresh as global vice president of strategy, a company statement said.

Shop Influenza virus NS1A-binding protein ELISA Kit, Recombinant Protein and Influenza virus NS1A-binding protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.

Copyright 2015 © EY Laboratories, Inc. All Rights Reserved. Reproduction of any materials from the site is strictly forbidden without permission. ...

Page contains details about Concanavalin A/bovine serum albumin-incorporated LA-conjugated Tetronic® 1107 micelles aggregates . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com

Schmidtke, J R.; Ray, P K.; and Simmons, R L., Effects of cell-bound phytohemagglutinin and concanavalin a on the mixed leucocyte response of mouse spleen cells in vitro. (1974). Subject Strain Bibliography 1974. 1861 ...

A method for the visualization of concanavalin A (Con A) binding sites by electron microscopy of glycol methacrylate sections is presented. This method, which is an application of the alkaline...

Magnetic resonance studies of concanavalin A: location of the binding site of alpha-methyl-D-mannopyranoside.: The longitudinal nuclear magnetic relaxation time

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TY - JOUR. T1 - An anti-inflammatory function for the complement anaphylatoxin C5a-binding protein, C5L2. AU - Gerard, Norma P.. AU - Lu, Bao. AU - Liu, Pixu. AU - Craig, Stewart. AU - Fujiwara, Yuko. AU - Okinaga, Shoji. AU - Gerard, Craig. PY - 2005/12/2. Y1 - 2005/12/2. N2 - C5L2 is an enigmatic serpentine receptor that is co-expressed with the C5a receptor on many cells including polymorphonuclear neutrophils. The apparent absence of coupling of C5L2 with G proteins suggests that this receptor may modulate the biological activity of C5a, perhaps by acting as a decoy receptor. Alternatively, C5L2 may affect C5a function through formation of a heteromeric complex with the C5aR, or it may utilize a G protein-independent signaling pathway. Here we show that in mice bearing a targeted deletion of C5L2, the biological activity of C5a/C5adesArg is enhanced both in vivo and in vitro. The biological role of C5L2 thus appears to be limiting to the pro-inflammatory response to the anaphylatoxin. ...

Sigma-Aldrich offers abstracts and full-text articles by [Sainan Li, Yujing Xia, Kan Chen, Jingjing Li, Tong Liu, Fan Wang, Jie Lu, Yingqun Zhou, Chuanyong Guo].

Citation: Tanna, S., Sahota, T., Taylor, M. et al. (2002). Covalent coupling of concanavalin A to a Carbopol 934P and 941P carrier in glucose-sensitive gels for delivery of insulin. Journal Pharmacy and Pharmacology, 54 (11) pp.1461-1469. ...

Benfords law was empirically tested against the numbers (up to the 10th digit) generated by a number of important distributions, including the uniform distribution, the exponential distribution, the half-normal distribution, the right-truncated normal, the normal distribution, the chi square distribution and the log normal distribution.[6] In addition to these the ratio distribution of two uniform distributions, the ratio distribution of two exponential distributions, the ratio distribution of two half-normal distributions, the ratio distribution of two right-truncated normal distributions, the ratio distribution of two chi-square distributions (the F distribution) and the log normal distribution were tested. The uniform distribution as might be expected does not obey Benfords law. In contrast, the ratio distribution of two uniform distributions is well described by Benfords law. Benfords law also describes the exponential distribution and the ratio distribution of two exponential ...

Department of Chemistry, UBC Faculty of Science. Vancouver Campus. 2036 Main Mall. Vancouver, BC Canada V6T 1Z1. Tel: 604.822.3266. Fax: 604.822.2847. ...

Disialoganglioside-GD2 from bovine brain (~ 95%, lyophilized powder, semisynthetic); Cell surface component from disialoganglioside-GD1b;

1. The luminol-dependent chemiluminescence of rat thymocytes responding to concanavalin A can be resolved into glucose-dependent and glucose-independent portions. 2. The glucose-dependent portion, supported by D-glucose and D-mannose oxidation, is inhibited by catalase (200 microgram/ml), amobarbital (1 mM) and hexose analogues that block D-glucose uptake. Thus concanavalin A may activate, transiently, an NAD(P)H oxidase that utilizes reducing equivalents derived from the oxidation of exogenous glucose to give dismutation products of O2- (including H2O2) as its major products. 3. The glucose-independent portion is inhibited by eicosa-5,8,11,14-tetraynoic acid but not by indomethacin. It may therefore be associated with the conversion of hydroperoxy intermediates of arachidonic acid metabolism to hydroxy products by the lipoxygenase pathway. 4. Preincubation of thymocytes for 18 h in serum-free medium enhances the subsequent chemiluminescent response to concanavalin A severalfold and evokes the ...

This study aimed to evaluate the effect of mesenchymal stem cell (MSC)-derived exosomes on an immune-induced liver injury model. MSCs show a unique function to modulate immune reaction although the molecular mechanisms are still under investigation. Exosomes are a nanoparticle containing microRNA and many ligands and are recognized as important factors secreted from MSC to express their function. This research is undertaken to evaluate the effect of MSC-derived exosome on concanavalin-A (con-A)-induced liver injury. Exosomes were collected from the supernatant of MSC from the bone marrow of C57B6 mice with ultracentrifugation. The collected exosomes or MSCs were injected intravenously into liver injury mice that had been prepared by the intravenous con-A injection. Liver and serum samples were collected 24 h later to evaluate the macro- and microscopic images, the alanine aminotransferase (ALT), and cytokine messenger RNA (mRNA) expression levels. Phenotypical change of non-parenchymal liver cells was

BioAssay record AID 196412 submitted by ChEMBL: Percent inhibition of Concanavalin A stimulated cell proliferation of rat spleen lymphocytes at 10 uM; ND is not determined.

Jul 06, 2015· For coarse aggregate 2 kg sample is adequate and for fine aggregate 05 kg sample is adequate The wet/moist sample is then weighed wet weight (W) Heated very gently on the frying pan or metal plate and stirred with a glass or metallic rod to maintain uniform distribution of heat, until the sheen disappears from the surface. ...

We recently identified acyl coenzyme A-binding proteins (ACBP)/diazepam binding inhibitor (DBI) being a book hunger aspect: a proteins thats upregulated in individual or murine weight problems which, if administered to mice, causes hyperphagy, obesity and adipogenesis. 13?C-glucose in liver organ and plasma (b), or entire body respirometry (c). Energy expenses (EE) and air consumption and skin tightening and creation (RQ?=?vCO2/vO2) were utilized to calculate fatty acidity oxidation. ACBP/DBI neutralization highly decreased the hyperphagic response induced by transient Rabbit Polyclonal to FPR1 hunger (24?h). As as 30 shortly?min after intraperitoneal shot of the anti-ACBP/DBI mAb, the activation of orexigenic neurons was inhibited, suggesting these results are mediated with the neutralization of peripheral (not central-nervous) ACBP/DBI because an antibody is expected should combination the brain bloodstream hurdle [8]. In given mice, ACBP/DBI neutralization triggered a transient and moderate ...

Abstract: Glucagon and concanavalin A were administered into cell cultures of mice peritoneal macrophages and human intima aorta simultaneously with atherogenous blood serum, obtained from patients with ischemic heart disease, or with acetylated and native low density lipo-proteins. Their effect was dissimilar: glucagon decreased accumulation of intracellular cholesterol and the rate of 3H-thymidine incorporation into these cells, while concanavalin A increased the patterns studied. Cellular lysosomes appear to participate in atherogenesis, these results suggest that regulation of lysosomal apparatus may occur at the step of secondary lysosomes formation ...

When molecules on the surface of cell are crosslinked, they are moved to one end of the cell to form a cap. This phenomenon, the process of which is called cap formation, was discovered in 1971 on lymphocytes and is a property of amoebae and all locomotory animal cells except sperm. The crosslinking is most easily achieved using a polyvalent antibody to a surface antigen on the cell. Cap formation can be visualised by attaching a fluorophore, such as fluorescein, to the antibody. The antibody is bound to the cell. If the antibody is non-crosslinking (such as a Fab antibody fragment), the bound antibody is uniformly distributed. This can be done at 0 °C, room temperature, or 37 °C. If the antibody is crosslinking and bound to the cells at 0 °C, the distribution of antibodies has a patchy appearance. These patches are two-dimensional precipitates of antigen-antibody complex and are quite analogous to the three-dimensional precipitates that form in solution. If cells with patches are warmed ...

The appearance of aberrant glycans on the tumor cell surface is one of the emerging hallmarks of cancer. Glycosylation is an important post-translation modification of proteins and lipids and is strongly affected by oncogenesis. Tumor-associated glycans have been extensively characterized regarding their composition and tumor-type specific expression patterns. Nevertheless whether and how tumor-associated glycans contribute to the observed immunomodulatory actions by tumors has not been extensively studied. Here, we provide a detailed overview of the current knowledge on how tumor-associated O-glycans affect the anti-tumor immune response, thereby focusing on truncated O-glycans present on epithelial tumors and mucins. These tumor-associated O-glycans and mucins bind a variety of lectin receptors on immune cells to facilitate the subsequently induction of tolerogenic immune responses. We, therefore, postulate that tumor-associated glycans not only support tumor growth, but also actively contribute to

Christina Eich graduated from the University of Bonn and joined the Tumour Immunology Department at the Radboud University in Nijmegen (The Netherlands) in 2007 for her PhD. During her PhD project and first year of postdoctoral research she explored how adhesion receptor signals orchestrate adhesion/trafficking of immune cells in vivo. She applied microscopy and proteomics to investigate how the molecular organization of the cell surface influences cell adhesion by studying the model adhesion receptor LFA-1. Her research demonstrated that nanoscale changes in the lipid composition of the plasma membrane affect receptor mobility and consequently immune cell function. Moreover, she demonstrated for the first time that LFA-1 interacting proteins change during cell differentiation, differentially regulating LFA-1 function in immune cells. During her second postdoctoral at the Erasmus Stem Cell Institute, Department of Cell biology, Erasmus Medical Center Rotterdam (The Netherlands), she studied the ...

Fingerprint Dive into the research topics of Cloned helper T lymphocytes exposed to interleukin 2 become unresponsive to antigen and concanavalin A but not to calcium ionophore and phorbol ester. Together they form a unique fingerprint. ...

Surolia, Avadhesha and Bachhawat, BK and Vithyathil, PJ and Podder, SK (1978) Unique subunit structure for Ricinus communis agglutinin. In: Indian Journal of Biochemistry & Biophysics, 15 (4). pp. 248-250. Full text not available from this repository. (Request a copy ...

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Lektiny jsou velká skupina proteinů neimunitního původu, které dokáží s vysokou mírou specifity rozpoznávat a vázat cukry, ať už volné nebo vázané na glykoproteinech nebo glykolipidech. Na rozdíl od glykosyltransferáz lektiny nekatalyzují žádnou chemickou reakci, tedy nevykazují enzymatickou aktivitu. Lektiny se účastní mnoha dějů, ve kterých je nutné specifické rozpoznávání (imunologické reakce, kontakt buněk ve tkáních, interakce patogenů s hostitelem atd.). První objevené lektiny byly pro jejich schopnost aglutinovat (shlukovat, srážet) červené krvinky pojmenovány aglutininy (WGA - wheat germ agglutinin, RCA - Ricinus communis agglutinin). ...

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1ONA: A structure of the complex between concanavalin A and methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside reveals two binding modes.

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我的實驗室對於闡明病原體引起細胞激素風暴以及致死的分子機轉，和確認預防免疫治療的潛在目標，以預防組織損傷及纖維化。我們從兩個方向探討這些問題:宿主與病原體交互作用以及免疫調節。. Host-Pathogen Interaction. 發炎反應的啟動是經由自然殺手細胞、單核球細胞、巨噬細胞、嗜中性白血球以及血小板上的先天免疫受體受到感染因子、外來抗原或是異常表現的內源性抗原的活化。因此，了解外源性以及內源性抗原在先天免疫反應的分子機轉將能夠開發新的治療策略以治療諸多人類疾病，例如感染症、自體免疫以及過敏反應。我們開發了先天免疫受體陣列(innate immunity receptor array / IIR-EIA)以及C型凝集素受體陣列(C-type lectin receptor array ...