OBJECTIVES: To investigate the possible association between vitamin D receptor genotype and bone mineral density in a large group of postmenopausal twins. DESIGN: Cross sectional twin study. SETTING: Twin population based in Britain. SUBJECTS: 95 dizygotic (non-identical) pairs of twins and 87 monozygotic (identical) pairs of twins aged 50-69 years, postmenopausal, and free of diseases affecting bone, recruited from a national register of twins and with a media campaign. MAIN OUTCOME MEASURES: Bone mineral density measured at the hip, lumbar spine, forearm, and for the whole body by dual energy x ray absorptiometry in relation to differences in the vitamin D receptor genotype. RESULTS: At all sites the values of bone density among dizygotic twins were more similar in those of the same vitamin D receptor genotype than in those of differing genotype, and the values in the former were closer to the correlations seen in monozygotic twins. Women with the genotype that made them at risk of osteoporotic

OBJECTIVES--To investigate the possible association between vitamin D receptor genotype and bone mineral density in a large group of postmenopausal twins. DESIGN--Cross sectional twin study. SETTING--Twin population based in Britain. SUBJECTS--95 dizygotic (non-identical) pairs of twins and 87 monozygotic (identical) pairs of twins aged 50-69 years, postmenopausal, and free of diseases affecting bone, recruited from a national register of twins and with a media campaign. MAIN OUTCOME MEASURES--Bone mineral density measured at the hip, lumbar spine, forearm, and for the whole body by dual energy x ray absorptiometry in relation to differences in the vitamin D receptor genotype. RESULTS--At all sites the values of bone density among dizygotic twins were more similar in those of the same vitamin D receptor genotype than in those of differing genotype, and the values in the former were closer to the correlations seen in monozygotic twins. Women with the genotype that made them at risk of osteoporotic

TY - JOUR. T1 - Sun exposure, vitamin D receptor gene polymorphisms, and breast cancer risk in a multiethnic population. AU - John, Esther M.. AU - Schwartz, Gary G.. AU - Koo, Jocelyn. AU - Wang, Wei. AU - Ingles, Sue A.. PY - 2007/12. Y1 - 2007/12. N2 - Considerable evidence indicates that vitamin D may reduce the risk of several cancers, including breast cancer. This study examined associations of breast cancer with sun exposure, the principal source of vitamin D, and vitamin D receptor gene (VDR) polymorphisms (FokI, TaqI, BglI) in a population-based case-control study of Hispanic, African-American, and non-Hispanic White women aged 35-79 years from the San Francisco Bay Area of California (1995-2003). In-person interviews were obtained for 1,788 newly diagnosed cases and 2,129 controls. Skin pigmentation measurements were taken on the upper underarm (a sun-protected site that measures constitutive pigmentation) and on the forehead (a sun-exposed site) using reflectometry. Biospecimens were ...

TY - JOUR. T1 - Sun exposure, vitamin D receptor gene polymorphisms, and risk of advanced prostate cancer. AU - John, Esther M.. AU - Schwartz, Gary G.. AU - Koo, Jocelyn. AU - Van Den Berg, David. AU - Ingles, Sue A.. PY - 2005/6/15. Y1 - 2005/6/15. N2 - Substantial experimental evidence indicates that the hormonal form of vitamin D promotes the differentiation and inhibits the proliferation, invasiveness, and metastasis of human prostatic cancer cells. Results from epidemiologic studies of vitamin D status and/or vitamin D receptor (VDR) polymorphisms and prostate cancer risk have been mixed. We conducted a population-based, case-control study of advanced prostate cancer among men ages 40 to 79 years from the San Francisco Bay area. Interview data on lifetime sun exposure and other risk factors were collected for 905 non-Hispanic White men (450 cases and 455 controls). Using a reflectometer, we measured constitutive skin pigmentation on the upper underarm (a sun-protected site) and facultative ...

Patients with insulin-dependent diabetes mellitus (IDDM) are at higher risk of developing osteoporosis. Among the genetic factors related to die development of osteoporosis, a possible association between vitamin D receptor (VDR) gene polymorphism and bone mineral density (BMD) has been described in some populations. We characterized the VDR gene polymorphism in a healthy adult Brazilian population and in a group of patients with IDDM and correlated these findings with densitometric values in both groups. the Brazilian population is characterized by an important racial heterogeneity and therefore is considered an ethnically heterogeneous population. We recruited 94 healthy adult Brazilian volunteers (63 women and 31 men), mean (+/- SD) age 32.4 +/- 6.5 years (range 18-39 years), and 78 patients with IDDM (33 women and 45 men) diagnosed before 18 years of age, mean (+/- SD) age 23.3 +/- 5.5 years (range 18-39 years). VDR genotype was assessed by polymerase chain reaction amplification followed by ...

Graves' (GD) hyperthyroidism induces accelerated bone turnover that leads to decreased bone mineral density (BMD). The role of the VDR gene in predisposition to primary osteoporosis has been recognized. Recent studies show associations between the VDR gene polymorphisms and susceptibility to autoimmune diseases. Here we analyzed if VDR gene polymorphisms: BsmI, ApaI, TaqI, and FokI may predispose women with Graves' hyperthyroidism to BMD reduction or to disease development. The subjects were 75 premenopausal female Polish patients with GD and 163 healthy women. The genotyping was performed by the use of the restriction fragment length polymorphism analysis (RFLP). We studied the association of the VDR polymorphisms and their haplotypes with patients' BMD and also SNPs and haplotypes association with Graves' disease. We found a strong linkage disequilibrium for the BsmI, ApaI, and TaqI polymorphims that formed three most frequent haplotypes in Graves' women: baT (47.9%), ...

We conducted this study to examine the relationship between VDR expression and mutations in PIK3CA and KRAS in colorectal cancer. Accumulating evidence indicates a substantial role of vitamin D in prevention of various forms of human cancer (1, 2, 17, 36-38). A potential link between VDR and the PI3K-AKT or RAS-MAPK pathway has been suggested (17, 18). Thus, examining VDR in colorectal cancer may shed lights on biological mechanisms of vitamin D action and its failure. We have found that VDR overexpression in colorectal cancer is significantly associated with both PIK3CA and KRAS mutations independent of other clinical and molecular features. Our data support the hypothesis that the VDR pathway interacts with the PI3K-AKT and RAS-MAPK pathways in colonic neoplastic cells.. Our resource of a large number of colorectal cancers derived from the two prospective cohort studies has enabled us to precisely estimate the frequency of colorectal cancers with a specific molecular feature (such as VDR ...

Dive into the research topics of A commentary on the VITAL study: Does vitamin D (receptor activation) protect against nephropathy in type 2 diabetes?. Together they form a unique fingerprint. ...

PBMC VDR expression is inversely associated with disease activity and inflammation in SLE patients, and VDR downregulation is likely driven by inflammation.

TY - JOUR. T1 - Vitamin D receptor polymorphisms and renal cancer risk in Central and Eastern Europe. AU - Karami, S.. AU - Brennan, P.. AU - Hung, R. J.. AU - Boffetta, P.. AU - Toro, J.. AU - Wilson, R. T.. AU - Zaridze, D.. AU - Navratilova, M.. AU - Chatterjee, N.. AU - Mates, D.. AU - Janout, V.. AU - Kollarova, H.. AU - Bencko, V.. AU - Szeszenia-Dabrowska, N.. AU - Holcatova, I.. AU - Moukeria, A.. AU - Welch, R.. AU - Chanock, S.. AU - Rothman, N.. AU - Chow, W. H.. AU - Moore, L. E.. N1 - Funding Information: This research was supported in part by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research. This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, and by European Commission INCO-COPERNICUS grant IC15-CT96-0313. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention ...

Low levels of 25-hydroxy vitamin D (25(OH)D) and polymorphisms in the vitamin D receptor gene (VDR) have been found separately to increase risk of breast cancer. The aim of this study was to determine whether low 25(OH)D levels, alone and in combination with BsmI VDR genotype, increased breast cance …

Background In patients with advanced non-small-cell lung cancer, vitamin D receptor (VDR) polymorphisms and haplotypes are reported to be associated with survival. We hypothesized that a similar association would be observed in patients with head and neck squamous-cell carcinoma (HNSCC). Methods In a post-hoc analysis of our previous prospective cohort study, VDR polymorphisms including Cdx2 G/A (rs11568820), FokI C/T (rs10735810), BsmI A/G (rs1544410), ApaI G/T (rs7976091), and TaqI T/C (rs731236) were genotyped by sequencing in 204 consecutive patients with HNSCC who underwent tumor resection. Progression-free survival was compared between VDR polymorphisms using Kaplan-Meier survival curves with log-rank tests and Cox proportional hazard models adjusting for age, gender, smoking status, primary tumor sites, postoperative stages, existence of residual tumor, and postoperative treatment with chemotherapy or radiotherapy. Results During a median follow-up of 1,047 days, tumor progression and death

PATINO-GARCIA, Brenda et al. Association between polymorphisms of the androgen and vitamin D receptor genes with prostate cancer risk in a Mexican population. Rev. invest. clín. [online]. 2007, vol.59, n.1, pp.25-31. ISSN 0034-8376.. Introduction. Prostate cancer (PCa) is a worldwide health issue, because of its high incidence and mortality. Its etiology is complex and includes certain risk factors such as age, hormonal status, ethnic origin and family history of PCa. Genetic predisposition is proposed as a major risk factor and there are several controversial reports on the association of PCa and gene polymorphism such as the receptors of the androgen receptor (AR) and the vitamin D (VDR). Objective. To evaluate the CAG triplets repetitions in the first exon of the AR and polymorphisms in the restriction site Taql in the VDR in Mexicans with PCa. Material and methods. A total of 68 Mexicans with histopathological diagnosis of PCa and 48 healthy Mexican with normal prostate specific antigen and ...

BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.

25-Hydroxyvitamin D3 Levels, BsmI Polymorphism and Insulin Resistance in Brazilian Amazonian Children. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.

Prostate cancer is the most commonly diagnosed cancer in men, but rates around the world vary greatly. Americans have the highest incidence rates in the world, 4 to 5 times those of Spain. The hypothesis of this thesis was that vitamin D metabolism is a factor in prostate cancer development and the aim was to examine differences in factors that modulate vitamin D metabolism between the US and Spain, specifically sunlight exposure and vitamin D receptor (VDR) polymorphisms. Through a search of papers that examined sunlight exposure and prevalence of VDR polymorphisms, we found that Spain had more ultraviolet exposure than the United States, but there was little difference in proportions of VDR polymorphisms between the two populations. TaqI, BsmI, and FokI polymorphisms of the VDR were studied. Proportions of TaqI and FokI were found to be equivalent between the two populations. The rates of BsmI polymorphisms were found to be statistically different, but the United States, not Spain, has higher ...

Polymorphisms in the vitamin D receptor (VDR) gene may be of etiological importance in determining cancer risk. The aim of this study was to assess the association between common VDR gene polymorphism

TY - JOUR. T1 - Using components of the vitamin D pathway to prevent and treat colon cancer. AU - Stubbins, Renee E.. AU - Hakeem, Aisha. AU - Núñez, Nomelí P.. PY - 2012/12. Y1 - 2012/12. N2 - The objective of this review was to analyze the components of vitamin D and their potential usefulness in preventing and treating colorectal cancer. The active form of vitamin D, 1α,25(OH2)D3, targets the wnt/β-catenin pathway by upregulating key tumor suppressor genes such as E-cadherin, which promotes an epithelial phenotype, but this is only possible when the vitamin D receptor (VDR) is present. Colorectal cell lines have shown that VDR expression levels decrease in the later stages of colon cancer. In colorectal cancers with low VDR expression, treatments to increase VDR expression could target alterations at the genomic and epigenomic levels by modulating transcription factors such as SNAIL1 and by utilizing histone deacetyltransferase inhibitors, respectively. Finally, epidemiological studies ...

1,25-Dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), the biologically active metabolite of Vitamin D(3), not only regulates bone and calcium metabolism but also exerts other biological activities, including immunomodulation via the nuclear Vitamin D receptor expressed in antigen-presenting cells and activated T cells. This regulation is mediated through interference with nuclear transcription factors such as NF-AT and NF-kappaB or by direct interaction with Vitamin D responsive elements in the promoter regions of cytokine genes. Dendritic cells (DCs) are primary targets for the immunomodulatory activity of 1,25(OH)(2)D(3), as indicated by inhibited DC differentiation and maturation, leading to down-regulated expression of MHC-II, costimulatory molecules and IL-12. Moreover, 1,25(OH)(2)D(3) enhances IL-10 production and promotes DC apoptosis. Together, these effects of 1,25(OH)(2)D(3) inhibit DC-dependent T cell activation. Immunomodulation by 1,25(OH)(2)D(3) and its analogs in vivo has been ...

We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...

The Wnt-β-catenin pathway is aberrantly activated in most colon cancers. DICKKOPF-1 (DKK-1) gene encodes an extracellular Wnt inhibitor that blocks the formation of signalling receptor complexes at the plasma membrane. We report that 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3], the most active vitamin D metabolite, increases the level of DKK-1 RNA and protein in human SW480-ADH colon cancer cells. This effect is dose dependent, slow and depends on the presence of a transcription-competent nuclear vitamin D receptor (VDR). Accordingly, 1,25(OH)2D3 activates a 2300 bp fragment of the human DKK-1 gene promoter. Chromatin immunoprecipitation assays revealed that 1,25(OH)2D3 treatment induced a pattern of histone modifications which is compatible with transcriptionally active chromatin. DKK-1 is expressed at high level in colon cancer cell lines with a differentiated phenotype such as Caco-2 or HT-29. Exogenous expression of E-cadherin into SW480-ADH cells results in a strong adhesive phenotype and a ...

Vitamin D receptor (VDR) participates in multiple immune functions. Here, we determined whether VDR gene-sequence variations are associated with intersubject differences in the risk of acquiring human immunodeficiency virus type 1 (HIV-1) infection. We assessed this in 460 males exposed to HIV-1 by injection drug use (335 infected and 125 uninfected) and 124 seronegative healthy subjects. Multilocus logistic regression analysis revealed haplotypes for rs11568820, rs4516035, rs10735810, rs1544410, and rs17878969 polymorphisms showing association with protection to HIV-1 infection (odds ratio, 0.4 [95% confidence interval, 0.22-0.721; P = .0025), which remained significant after correction for multiple testing. We infer that VDR haplotypes might influence the risk of HIV-1 acquisition ...

Genome- and transcriptome-wide data has significantly increased the amount of available information about primary 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) target genes in cancer cell models, such as human THP-1 myelomonocytic leukemia cells. In this study, we investigated the genes G0S2, CDKN1A and MYC as master examples of primary vitamin D receptor (VDR) targets being involved in the control of cellular proliferation. The chromosomal domains of G0S2 and CDKN1A are 140-170 kb in size and contain one and three VDR binding sites, respectively. This is rather compact compared to the MYC locus that is 15 times larger and accommodates four VDR binding sites. All eight VDR binding sites were studied by chromatin immunoprecipitation in THP-1 cells. Interestingly, the site closest to the transcription start site of the down-regulated MYC gene showed 1,25(OH)2D3-dependent reduction of VDR binding and is not associated with open chromatin. Four of the other seven VDR binding regions contain a typical DR3-type VDR

Tacalcitol | VDR agonist | PRI 2191 | 1α,24-Dihydroxycholecalciferol | PRI2191 | CAS [57333-96-7] | Axon 2516 | Axon Ligand™ with >98% purity available from supplier Axon Medchem, prime source of life science reagents for your research

Thank you for sharing this Cancer Epidemiology, Biomarkers & Prevention article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...

Lee, Laura R. et al Progesterone Enhances Calcitriol Antitumor Activity by Upregulating Vitamin D Receptor Expression and Promoting Apoptosis in Endometrial Cancer Cells. Cancer Prevention Research (2013): . Web. 22 Feb. 2020. ...

We hypothesized that methylation of VDR may be population specific and that the combination of differential methylation and genetic variation may characterize tuberculosis (TB) predisposition. We use bisulfite conversion and/or pyrosequencing to analyze the methylation status of 17 CpGs of VDR and to genotype 7 SNPs in the 3 CpG Island (CpG island [CGI] 1060), including the commonly studied SNPs ApaI (rs7975232) and TaqI (rs731236 ...

19.70. Even though vitamins and supplements may seem completely natural and safe, that is not always the case for specific individuals. A standard multivitamin is okay for most people who take a supplement. Many supplements are advertised as being specially designed for men or for women or for certain age groups. Do sugar bear hair vitamins make hair shine, nails stronger, and help both to grow? Look for generic brands that contain the same amount of vitamins and minerals as the name brand. Recently, the association with vitamin b and pd is getting more and more attention. A few small clinical trials have tested the treatment effect of alpha-lipoic acid given either as a supplement or intravenously. However, some people benefit from taking a b-complex supplement. Can lysine, vitamin b-12, or other vitamins or minerals help prevent or reduce canker sores? Vitamin d modulates growth and differentiation of keratinocytes through binding to the nuclear vitamin d receptor (Vdr). As mentioned above, ...

1α,25-dihydroxyvitamin D3 signals via the Vitamin D Receptor (VDR). Higher serum vitamin D is associated with thinner primary melanoma and better outcome, although a causal mechanism has not been established. As melanoma patients commonly avoid sun exposure, and consequent vitamin D deficiency might worsen outcomes, we interrogated 703 primary melanoma transcriptomes to understand the role of vitamin D-VDR signalling and replicated the findings in TCGA metastases. VDR expression was independently protective for melanoma death in both primary and metastatic disease. High tumor VDR expression was associated with upregulation of pathways mediating anti-tumor immunity and correspondingly with higher imputed immune cell scores and histologically detected tumor infiltrating lymphocytes (TILs). High VDR expressing tumors had downregulation of proliferative pathways, notably Wnt/beta-catenin signaling. Deleterious low VDR levels resulted from promoter methylation and gene deletion in metastases. ...

TY - JOUR. T1 - Involvement of vitamin D receptor in the intestinal induction of human ABCB1. AU - Tachibana, Shuko. AU - Yoshinari, Kouichi. AU - Chikada, Tsubasa. AU - Toriyabe, Takayoshi. AU - Nagata, Kiyoshi. AU - Yamazoe, Yasushi. PY - 2009/8. Y1 - 2009/8. N2 - ABCB1 (P-glycoprotein) is an efflux transporter that limits the cellular uptake levels of various drugs in intestine, brain, and other tissues. The expression of human ABCB1 has recently been reported to be under the control of nuclear receptor NR1I subfamily members, pregnane X receptor (PXR, NR1I2) and constitutive androstane receptor (CAR, NR1I3). Here, we have investigated the involvement of another NR1I member, vitamin D receptor (VDR, NR1I1), in ABCB1 expression. In the human colorectal adenocarcinoma cell line LS174T, which abundantly expresses VDR, both 1α,25-dihydroxyvitamin D3 (1,25-VD3) and lithocholic acid (LCA) increased ABCB1 mRNA levels. Reporter gene assays in LS174T cells with constructs containing various lengths ...

We evaluated the role of VDR in the activation of the renin angiotensin system (RAS) in morphine-induced T cell loss. Morphine treated human T cells displayed down regulation of VDR and the activation of the RAS. On the other hand, a VDR agonist (EB1089) enhanced T cell VDR expression both under basal and morphine-stimulated states. Since T cells with silenced VDR displayed the activation of the RAS, whereas, activation of the VDR was associated with down regulation of the RAS, it appears that morphine-induced T cell RAS activation was dependent on the VDR status. Morphine also enhanced reactive oxygen species (ROS) generation in a dose dependent manner; however, this effect of morphine was inhibited by an opiate receptor antagonist, naltrexone. These findings confirmed the role of opiate receptors in morphine-induced ROS generation. Interestingly, the activation of VDR as well as blockade of Ang II (by losartan, an AT1 receptor blocker) also inhibited morphine-induced T cell ROS generation. ...

Open Repository is based on and contributes to DSpace the open source tool for the management of digital assets. Open Repository is a service operated by Atmire. ...

Vitamin D Receptor ELISA Kits für viele Reaktivitäten. Chemical, Huhn, Rind (Kuh) und weitere. Vitamin D Receptor ELISA Kits vergleichen und bestellen.

The CC genotype of the vitamin D receptor (VDR) polymorphism TaqI rs731236 has previously been associated with a higher risk of developing myopathy compared to TT carriers. However, the mechanistic role of this polymorphism in skeletal muscle is not well defined. The effects of vitamin D on patients genotyped for the VDR polymorphism TaqI rs731236, comparing CC and TT carriers were evaluated. Primary human myoblasts isolated from 4 CC carriers were compared with myoblasts isolated from four TT carriers and treated with vitamin D in vitro. A dose-dependent inhibitory effect on myoblast proliferation and differentiation was observed concurrent with modifications of key myogenic regulatory factors. RNA sequencing revealed a vitamin D dose-response gene signature enriched with a higher number of VDR-responsive elements (VDREs) per gene. Interestingly, the greater the expression of muscle differentiation markers in myoblasts, the more pronounced was the vitamin D-mediated response to suppress genes ...

Serum calcium levels are tightly controlled by an integrated hormone-controlled system that involves active vitamin D [1,25(OH)2D], which can elicit calcium mobilization from bone when intestinal calcium absorption is decreased. The skeletal adaptations, however, are still poorly characterized. To gain insight into these issues, we analyzed the consequences of specific vitamin D receptor (Vdr) inactivation in the intestine and in mature osteoblasts on calcium and bone homeostasis. We report here that decreased intestinal calcium absorption in intestine-specific Vdr knockout mice resulted in severely reduced skeletal calcium levels so as to ensure normal levels of calcium in the serum. Furthermore, increased 1,25(OH)2D levels not only stimulated bone turnover, leading to osteopenia, but also suppressed bone matrix mineralization. This resulted in extensive hyperosteoidosis, also surrounding the osteocytes, and hypomineralization of the entire bone cortex, which may have contributed to the ...

Depression has been linked to (lack of) sunlight exposure and raised intracellular calcium levels. In this pathway, the role of the vitamin D pathway in calcium signaling in depression is shown. Active 1,25-dihydroxy-vitamin D3 is formed from 7-dehydrocholesterol by multiple conversion steps. It associates with RXR and VDR in the nucleus, which stimulates transcription of VDR target genes, resulting in lower intracellular calcium levels. Less sunlight exposure results in lower 1,25-dihydroxyvitamin D3 levels and therefore higher calcium levels ...

The vitamin D receptor gene encodes the nuclear hormone receptor for vitamin D3 and belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. In light of evidence for promoter methylation of the vitamin D receptor in the control of the expression of this gene, we have designed methylation assays that cover many regions of this gene in order to determine its methylation profile in normal individuals, ovarian tumors and ovarian cell lines. We have isolated the DNA from ten tumor tissues and their adjacent normal tissue from four different tumor types; ovarian, breast, colon and cervical. By utilizing a combination of bisulfite sequencing, SNP genotyping, and expression analysis, we show ...

Role of vitamin D receptor in the antiproliferative effects of calcitriol in tumor-derived endothelial cells and tumor angiogenesis in vivo.

BioAssay record AID 602200 submitted by NCGC: qHTS for Inhibitors of the vitamin D receptor (VDR): Hit Validation using a Fluorescein Assay.

BioAssay record AID 602202 submitted by NCGC: qHTS for Inhibitors of the vitamin D receptor (VDR): Hit Validation using a Beta-Lactamase Assay.

Here are some articles I found that illustrates 2 points - Firstly that there are other no pain no gain treatment protocols out there, in case anybody was doubting the wisdom of following MP. The term paradoxical pharmacology has been coined for these, and in one sense I think MP can join the list, after all it appears at first sight counter intuitive to make use of a VDR agonist (benicar) treatment when TH1 disease sufferers are already awash with a VDR agonist (1,25 D ...

I would like if someone send me information about vitamin D receptor localization in material fixed in 10% formalin and paraffin block. With compliments. Fernando Capela e Silva Departamento de Biologia Universidade de vora Apartado 94 7002-554 vora PORTUGAL Telephone: 266 760 881 Fax: 266 711 231 E-mail: [email protected] http://home.dbio.uevora.pt/~fcs/FCAPELAeSILVA.htm ...

2605 Background: Vitamin D receptors (VDR) are expressed on the surface of the epithelial ovarian cells and evidence from in vitro and animal models suggests an estrogen-dependent influence of VDR on female reproduction. We investigated the association of ovarian cancer risk with four polymorphisms in the VDR gene: the rs10735810 polymorphism (FokI) in the VDR promoter that alters the first start codon and three polymorphisms in the in 3 untranslated region, rs1544410 (BsmI), rs7975232 (ApaI) and rs731236 (TaqI), known to alter transcriptional activity and mRNA stability.. Methods:.Interviews and DNA samples were collected in a population-based case-control study of 313 women with epithelial ovarian carcinoma and 574 controls frequency-matched to cases on age and ethnicity. Genotyping was performed with the 5 nuclease discrimination assay. The associations of these genetic polymorphisms with ovarian carcinoma risk and their interaction with other hormone-related risk factors were studied using ...

P53 and its family members have been implicated in the direct regulation of the vitamin D receptor (VDR). Vitamin D- and p53-signaling pathways have a significant impact on spontaneous or carcinogen-induced malignant transformation of cells, with VDR and p53 representing important tumor suppressors. VDR and the p53/p63/p73 proteins all function typically as receptors or sensors that turn into transcriptional regulators upon stimulus, with the main difference being that the nuclear VDR is activated as a transcription factor after binding its naturally occurring ligand 1,25-dihydroxyvitamin D with high affinity while the p53 family of transcription factors, mostly in the nucleoplasm, responds to a large number of alterations in cell homeostasis commonly referred to as stress. An increasing body of evidence now convincingly demonstrates a cross-talk between vitamin D- and p53-signaling that occurs at different levels, has genome-wide implications and that should be of high importance for many malignancies,

SNP in the vitamin D receptor (VDR) gene is associated with risk of lower respiratory infections. The influence of genetic variation in the vitamin D pathway resulting in susceptibility to upper respiratory infections (URI) has not been investigated. We evaluated the influence of thirty-three SNP in eleven vitamin D pathway genes (DBP, DHCR7, RXRA, CYP2R1, CYP27B1, CYP24A1, CYP3A4, CYP27A1, LRP2, CUBN and VDR) resulting in URI risk in 725 adults in London, UK, using an additive model with adjustment for potential confounders and correction for multiple comparisons. Significant associations in this cohort were investigated in a validation cohort of 737 children in Manchester, UK. In all, three SNP in VDR (rs4334089, rs11568820 and rs7970314) and one SNP in CYP3A4 (rs2740574) were associated with risk of URI in the discovery cohort after adjusting for potential confounders and correcting for multiple comparisons (adjusted incidence rate ratio per additional minor allele ≥1·15, Pfor trend ...

Abstract: DESCRIPTION (provided by applicant): Investigations in this proposal are focused on identifying the molecular basis for the ligand dependent and independent actions of the VDR, using the skin as a model system. Like humans with VDR mutations, VDR null mice develop alopecia. We have demonstrated that the ligand-independent actions of the VDR are required for hair follicle keratinocyte stem cell (KSC) function, normal hair cycling and canonical Wnt (cWnt) signaling in keratinocytes. Interfering with cWnt signaling also impairs KSC function. Studies examining the interactions of the VDR with effectors of canonical Wnt signaling demonstrate that the VDR interacts with Lef1 via its DNA binding domain and that the VDR interacting domain of Lef1 is independent of its ¿-catenin binding domain. We will examine the functional consequences of impairing VDR-Lef1 interactions in keratinocytes and determine if the interaction between these two proteins is direct, or involves additional factors. ...

Duffy, MM,McNicholas, BA,Monaghan, DA,Hanley, SA,McMahon, JM,Pindjakova, J,Alagesan, S,Fearnhead, HO,Griffin, MD (2014) Mesenchymal stem cells and a vitamin D receptor agonist additively suppress T helper 17 cells and the related inflammatory response in the kidney. American Journal Of Physiology-Renal Physiology, 307 :1412-1426 [DOI] [Details] ...

BioTek 应用手册, 12/13 2017, An Image-Based Method to Detect and Quantify T Cell Mediated Cytotoxicity of 2D and 3D Target Cell Models

Abstract Aim: Serum osteocalcin was shown in a previous study on first trimester pregnant women to correlate with bone density and to distinguish between fast and slow bone losers. The objective of the present study is to examine whether serum osteocalcin is related to vitamin D receptor (VDR) BsmI polymorphism in pregnant women. Study design: We determined osteocalcin serum levels and VDR BsmI genotype in 97 healthy first trimester pregnant women consecutively recruited during six months. Results: BB (21%), Bb (38%) and bb (41%) genotypes showed similar osteocalcin serum levels. However, in primigravidas (ns38) the BB genotype was significantly associated with higher mean osteocalcin level (9.67 ng/mL) than the Bb (8.07 ng/mL) and the bb genotype (8.14 ng/mL), respectively (P-0.05). The VDR genotype was the only independent parameter to correlate with serum osteocalcin (P-0.05). Conclusion: Only primigravidas show in the first trimester a relation between the bone formation parameter serum ...

Purpose : To investigate the effects on Vitamin D Receptor (VDR) knockout and 1,25- and 24,25-dihyroxyvitamin D3 (Vit D3) on cultured mouse corneal epithelial cell proliferation and on the Vit D activating enzyme CYP27B1 and inactivating enzyme CYP24A1. We also examined effects of vitamin D on mouse corneal epithelial intracellular calcium levels. Methods : Cultured mouse corneal epithelial cell (MCEC) proliferation was measured by reduction of MTT, which corresponds to the living cell number and metabolic activity. Total RNA and protein were isolated from mouse corneas and cultured mouse primary corneal epithelial cells. Transcript levels of CYP24A1 and CYP27B1 were assessed by qPCR, and Western blotting was used to detect CYP24A1 and CYP27B1 protein levels. Corneal epithelial calcium levels were recorded in excised, ex vivo mouse corneas using the calcium-sensitive dye Cal-520® and multi-photon microscopy. Results : 24,25-Vit D3 (50 nM) significantly increased proliferation in corneal ...

The mechanism of action of calcitriol in the treatment of psoriasis is accounted for by their antiproliferative activity for keratinocytes and their stimulation of epidermal cell differentiation. The anticarcinogenic activity of the active form of Calcitriol appears to be correlated with cellular vitamin D receptor (VDR) levels. Vitamin D receptors belong to the superfamily of steroid-hormone zinc-finger receptors. VDRs selectively bind 1,25-(OH)2-D3 and retinoic acid X receptor (RXR) to form a heterodimeric complex that interacts with specific DNA sequences known as vitamin D-responsive elements. VDRs are ligand-activated transcription factors. The receptors activate or repress the transcription of target genes upon binding their respective ligands. It is thought that the anticarcinogenic effect of Calcitriol is mediated via VDRs in cancer cells. The immunomodulatory activity of calcitriol is thought to be mediated by vitamin D receptors (VDRs) which are expressed constitutively in monocytes ...

Vitamin D endocrine system and osteoblasts.: The interaction between vitamin D and osteoblasts is complex. In the current review we will give an overview of the

BACKGROUND: Chronic hepatitis C infection is a major cause of end-stage liver disease. Therapy outcome is influenced by 25-OH vitamin D deficiency. To further address this observation, our study investigates the impact of the vitamin D receptor (NR1I1) haplotype and combined effects of plasma vitamin D levels in a well-described cohort of hepatitis C patients. METHODS: A total of 155 chronic hepatitis C patients were recruited from the Swiss Hepatitis C Cohort Study for NR1I1 genotyping and plasma 25-OH vitamin D level measurement. NR1I1 genotype data and combined effects of plasma 25-OH vitamin D level were analysed regarding therapy response (sustained virological response). RESULTS: A strong association was observed between therapy non-response and the NR1I1 CCA (bAt) haplotype consisting of rs1544410 (BsmI) C, rs7975232 (ApaI) C and rs731236 (TaqI) A alleles. Of the HCV patients carrying the CCA haplotype, 50.3% were non-responders (odds ratio [OR] 1.69, 95% CI 1.07, 2.67; P=0.028). A ...

TY - JOUR. T1 - Normal vitamin D receptor concentration and responsiveness to 1,25-dihydroxyvitamin D3 in skin fibroblasts from patients with absorptive hypercalciuria. AU - Zerwekh, J. E.. AU - Reed, B. Y.. AU - Heller, H. J.. AU - Gonzalez, G. B.. AU - Haussler, M. R.. AU - Pak, C. Y C. PY - 1998/1/1. Y1 - 1998/1/1. N2 - To evaluate whether there is an increase in vitamin D receptor (VDR) concentration which could raise intestinal calcium absorption in absorptive hypercalciuric (AH) patients and promote hypercalciuria, we measured VDR concentration and VDR mRNA levels in skin fibroblasts from 16 patients with AH and 17 age-matched normal subjects before and following a 16-hour incubation in the presence of 10-8 M 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. There were no significant differences in VDR concentration between normal subjects and AH patients in the basal state (30 ± 11 vs. 30 ± 15 ng/mg protein, respectively) or following 1,25(OH)2D3-mediated upregulation (43 ± 18 vs. 42 ± 16 ng/mg ...

In a 12-week randomized controlled trial ( double-blind study), based on data on serum 25(OH)D changes from other studies (35), to achieve 1 standard deviation difference in circulating 25(OH)D with two-sided alpha of 0.05 and a beta of 0.2 after a 12-week intervention period, the sample size was calculated 40 individuals per treatment group. Considering 3 polymorphic variant groups and one control group which receives plain yogurt drink, 160 subjects are needed. Allowing for 10% attrition over 12 weeks of intervention, a total of 180 subjects are required. In treatment groups, all patients receive 500 ml per day of low-fat (1%) Yogurt Drink fortified with approximately 500IU of vitamin D3 . Control group receives 500 ml per day of plain (unfortified) Yogurt Drink. Metabolic, inflammatory and oxidative stress markers are evaluated at the beginning and in the end of the interventional period ...

Vitamin D is produced in the skin upon sun-exposure or obtained through the diet. Vitamin D is hydroxylated to 25-hydroxyvitamin D (25(OH)D) in the liver and to the active form 1,25-dihydroxyvitamin D (1,25(OH) 2 D) in the kidneys. To exert its effect 1,25(OH) 2 D has to bind to the nuclear vitamin D receptor VDR. Lack of vitamin D leads to rickets in children and to osteomalacia in adults. 25(OH)D is used as a marker of a subjects vitamin D status. Low serum 25(OH)D levels are associated with a number of diseases, risk factors for disease and increased mortality ...

TY - JOUR. T1 - Vitamin D receptor quantitation in human blood mononuclear cells in health and disease. AU - Zerwekh, J. E.. AU - Yu, X. P.. AU - Breslau, N. A.. AU - Manolagas, S.. AU - Pak, C. Y C. PY - 1993/10. Y1 - 1993/10. N2 - Vitamin D receptor (VDR) concentration was quantitated in human peripheral blood mononuclear cells (PBMC) from patients with absorptive hypercalciuria (AH) and patients with high 1,25(OH)2D3 due to acquired or transient disease states and the results compared to those in normal subjects. VDR concentration in resting cells was not different between the three groups and represented constitutive receptor expression of monocytes. Following activation with phytohemagglutinin, patients with hypercalcitriolemia demonstrated significantly greater VDR concentrations. Patients with AH demonstrated a normal value for the group, but 6 patients had significantly greater concentrations of VDR despite normal plasma 1,25(OH)2D3 in four of the patients. Proliferation, as assessed ...

The researchers explain that vitamin D intake and serum concentrations of its metabolites have been associated with a decreased risk of developing breast cancer. The vitamin plays a known role in controlling calcium levels and influences the differentiation of cells and so could play a part in preventing the runaway proliferation of cells characteristic with cancer.. Previous studies regarding the association between vitamin D and breast cancer have been inconsistent in their conclusions.. Chang-Claude and her colleagues have investigated variations in the gene encoding the vitamin D receptor protein. They found that there were no differences in the biomarker for vitamin D, 25-hydroxyvitamin D, between women with two well-known genetic variations, the polymorphisms FokI and TaqI, and two functional putative variants, VDR-5132 and Cdx2, in the gene for the receptor. Moreover, they found no relationship between the presence of these polymorphisms and overall risk of postmenopausal breast ...

In an unrelated vitamin D study, researchers have identified vitamin D receptors (VDR) on 2776 gene positions and also found 229 genes that were activated in response to vitamin D. Vitamin D receptors are proteins that become activated by vitamin D. Activated VDR can then attach to specific sites on DNA which result in the expression of certain gene traits and/or genetic products such as enzymes. VDR binding sites were densely populated near autoimmune and cancer associated genes which may imply the importance vitamin D can play in autoimmune disease and cancer prevention. ...

Osteosarcoma (OS) is the most common type of bone cancer in children and adolescents. With the current treatment approaches, the survival rate is 60-70%. Therapies for treating OS have remained the same over the past thirty years. There is a need for developing more effective therapies, which can significantly improve the survival of the patients who do not respond well to current therapeutic strategies. Epidemiological evidence indicates impaired regulation of cell proliferation and survival appears to be a key event in the etiology of the disease. Previous in vitro and in vivo studies suggest that 1α,25-dihydroxyvitamin D3 (calcitriol or 1,25D) has significant antineoplastic activity by inhibiting cell proliferation and inducing differentiation and apoptosis in breast, prostate, colon, skin, and brain cancer. The molecular mechanisms for calcitriol-mediated cancer prevention are still unknown. In particular, the role of calcitriol in modulating OS needs to be investigated. The primary ...

The research team, which included scientists from the University of Medicine and Dentistry of New Jersey and Stanford University, noted after vitamin D binds to the receptor, also blocks a protein called NFAT needed to turn on the gene. Without the gene on, levels of IL-17 fall. Vitamin D also does another job by turning on a gene that produces suppressive T cells to fight destructive IL-17-production that occurs elsewhere.. According to the authors, in mouse models with EAE, vitamin D, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] diminishes paralysis and progression of the disease and reduces IL-17A-secreting CD4+ T cells in the periphery and central nervous system (CNS).. Identification of the vitamin D pathway could lead to new treatments not only for multiple sclerosis, but for other autoimmune diseases include type 1 diabetes, rheumatoid arthritis and skin disorders. The authors concluded, Our results describe novel mechanisms and new concepts with regard to vitamin D and the immune system ...

Leica BLK3D Target Calibration Plate 877100 - Leica BLK3D Target Calibration Plate Target Calibration Plate for BLK3D: Size A3 Used for checking and adjusting the BLK3D.

Calcitriol Receptors: Proteins, usually found in the cytoplasm, that specifically bind calcitriol, migrate to the nucleus, and regulate transcription of specific segments of DNA with the participation of D receptor interacting proteins (called DRIP). Vitamin D is converted in the liver and kidney to calcitriol and ultimately acts through these receptors.

June 21 (Sunday) 14:00~ Registration 15:30~15:50 ,Ocean Hall, Opening Opening Remarks: F. Peter Guengerich (Vanderbilt University School of Medicine) 15:50-16:45 Plenary Lecture ,Ocean Hall, Shigeaki Kato (University of Tokyo): Nuclear vitamin D receptor-regulated expression of the human CYP27B1 gene mediates the DNA methylation/demetylation Session 1 Bioinformatics Chairs: Osamu Gotoh, Daniele Werck-Reichhart S1-L1: 17:00-17:30 Osamu Gotoh (Kyoto University): Birth and death of P450 genes in metazoan S1-L2: 17:30-18:00 Daniele Werck-Reichhart (CNRS-University, de Strasbourg): P450 evolution and neofunctionalization in plants: emergence of a novel phenolic pathway in Brassicaceae S1-L3: 18:00-18:30 Jongsun Park (Seoul National University): Integrated bioinformatics platforms for identifying and analyzing cytochrome P450s from diverse genomic resources S1-L4: 18:30-19:00 Masanori Arita (University of Tokyo): Data management of cross-disciplinary June 22 (Monday) 9:00~13:00 , Summit Hall , Session ...

Nuclear receptors are the central components of the complex signaling processes mediated by nuclear hormones. The specific interaction of a nuclear receptor, such as the VDR, with response elements of promoter regions determines which genes will be regulated by the respective nuclear hormone (i.e., in the case of VDR, which genes are primary VD-responding genes). The complex formation between VDR-RXR heterodimers and their specific VDREs is therefore a central molecular step in the specific activation of VD-responding genes. Investigation of the selective modulation of this complex formation on different types of VDREs by VDR ligands was therefore the subject of this study.. The selective biological profile of EB1089 [i.e., its potent antiproliferative potential combined with a reduced calcemic effect (Nayeri et al., 1995; Danielsson et al., 1997)] was associated with the higher selectivity (approximately 15-fold) of the analogs to activate IP9-type VDREs than DR3-type VDREs (Nayeri et al., ...

Results Clinical signs and symptoms of chronic arthritis were aggravated in hTNFtg mice lacking functional VDR signalling. Moreover, synovial inflammation was clearly increased in VDR−/−hTNFtg mice as compared to hTNFtg mice and was associated with an increased macrophage influx in inflamed joints. In vitro, VDR-deficient monocytes were proinflammatory and hyper-responsive to TNF stimulation associated with prolonged mitogen-activated protein kinase activation and cytokine secretion. Also, VDR−/− monocytes showed enhanced potential to differentiate into bone resorbing osteoclasts in vitro. In line, VDR−/−hTNFtg mice had significantly increased cartilage damage and synovial bone erosions.. ...

AB - A fundamental dilemma of steroid hormone regulation is how specific transcription is attained in vivo when several receptors recognize the same DNA sequence in vitro. We have identified an enhancer of the mouse sex-limited protein (Slp) gene that

Within the human digestive tract is a teeming mass of hundreds of types of bacteria, a potpourri of microbes numbering in the trillions that help us digest food and keep bad bacteria in check.

Calcitriol ,Calcitriol manufacturer,Calcitriol CAS No 32222-06-3,Calcitriol Molecular Formula C27H44O3,Calcitriol Synonyms (1R,3S)-5-[2-[(1R,3aR,7aS)-1-[(2R)-6-hydroxy-6-methyl-heptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H- inden-4-ylidene]ethylidene]-4-methylidene-cyclohexane-1,3-diol,Calcitriol Molecular weight 416.64 g/mol,Calcitriol manufacturing by A.S.Joshi & Company

Sleep Well - This is the core phrase of melatonin sleep aid! Yes, if you have heard about it then you must know that this is the natural hormone

Para que sirve el calcitriol 0.25 mcg, can i buy calcitriol over the counter, calcitriol 0.25 mg dosage, calcitriol 0.50 mcg precio, calcitriol farmacia san pablo, calcitriol 0.25 mcg capsules

Meta-analysis is a statistical approach that combines the results of multiple scientific studies. Although many genetic studies have been conducted to discovery genetic risk factors for T1D, combining the results from these separated studies by using meta-analysis could lead to a higher statistical power and more robust point estimate. In this study, meta-analysis was performed on seven T1D case/control bio-sets extracted from four recent studies. T1D target genes from meta-analysis were sorted by gene score, which is based on the statistical significance and consistency of the gene across the queried bio-sets. Meta-analysis results suggested six top risk genes for T1D (Score ≥ 85), and four of them are novel according to a recently updated database T1D_GD. Further analyses were conducted to study the possible correlation between T1D and these six genes, with more efforts focused on the four novel ones.. Analysis using T1D_GD showed that the two known T1D target genes, HLA-DQB1 and OAS1, are ...

How To Order Calcitriol Online In Canada 2018, How Much Does A Calcitriol Pill Cost, Cheap Calcitriol Extra Order Generic Calcitriol 0.25mg . Cheapest Calcitri…

Of in the blood interfere with vitamin D receptor binds to beneficial minerals such as heart disease and stroke. People of south Asian, Chinese, African-Caribbean or Middle Eastern descent are at higher risk for developing type 2 diabetes and gastrointestinal tract.. This helps increase stability and reactivity in extracts, the synergy in herbs and supplements as being practically important.. ...

Are you putting on weight, or getting tender breasts? There are many signs of oestrogen dominance and hormone imbalance so check the list to see what may be

FGF23-null mice have a short lifespan and develop features resembling premature ageing. This appears to be a vitamin D-mediated process similar to that observed in klohto (KL) mice [1853]. KL has been shown to be an essential cofactor for activation of FGF signaling by FGF23 [1851]. Though inconclusive at present, FGF23 might play some role in ageing. ...

Bio-identical hormones from by David Ghozland, M.D. can treat fatigue, irritability, and menopause symptoms as well as increase sexual desires and energy!

Patient Advocates aim is to help people regain their confidence and vitality for living by providing natural individualised hormonal balance.

Depression sufferers around the world may have a source of hope as researchers claim to have found a breakthrough way to treat the disorder.

online calcitriol purchases safe Another technique for some short term relief is to get 2 liters of warm water mixed with 1/2 cup of cornstarch and 1/2 cup baking soda ...

Recently active Calcitriol forums and community discussion threads. Post your question or story about Calcitriol and connect with others who have experience with the same medications. No registration is required and your identity remains anonymous.

78782-99-7;Calcitriol D6 Quality Supplierpharmaceutical chemical(cas 78782-99-7),LocatedNew JerseyMonmouth junction,Supply78782-99-7;Calcitriol D6。

This is a very natural hormone thought to be a building block for all the other steroid hormones. It certainly helps with all round well-being, but as we get older its productionRead More…. ...

Rocaltrol Calcitriol 0.25Mg Où En Acheter - Acheter Rocaltrol En Fr Rocaltrol En Canada Prix Rocaltrol Naturel Pas Cher, Forum Acheter Du Rocaltrol 0.25 Sur I…