TY - JOUR. T1 - A novel organ culture model of a joint for the evaluation of static and dynamic load on articular cartilage. AU - Lin, Y. C.. AU - Hall, A. C.. AU - Simpson, A. H.R.W.. PY - 2018/3/1. Y1 - 2018/3/1. N2 - Objectives: The purpose of this study was to create a novel ex vivo organ culture model for evaluating the effects of static and dynamic load on cartilage. Methods: The metatarsophalangeal joints of 12 fresh cadaveric bovine feet were skinned and dissected aseptically, and cultured for up to four weeks. Dynamic movement was applied using a custom-made machine on six joints, with the others cultured under static conditions. Chondrocyte viability and matrix glycosaminoglycan (GAG) content were evaluated by the cell viability probes, 5-chloromethylfluorescein diacetate (CMFDA) and propidium iodide (PI), and dimethylmethylene blue (DMMB) assay, respectively. Results: Chondrocyte viability in the static model decreased significantly from 89.9% (sd 2.5%) (Day 0) to 66.5% (sd 13.1%) ...

Non-typhoidal Salmonella is an important bacterial pathogen causing worldwide morbidity and mortality. Early interactions between Salmonella Typhimurium and intestinal epithelium have been demonstrated in animal models, but little is known in humans. After HEp-2 cell infection and extraction of genomic DNA using 1,440 transposon mutants of Salmonella Typhimurium, Transposon Directed Insertion-site Sequencing (TraDIS) identified 6 novel Salmonella virulence genomic loci responsible for adhesion/invasion (intergenic sucD-cydA, glyA, yqiC, wzxE, and rfaI) and intracellular replication (speG). The aims of this project were to a) create the single-gene mutants by site-directed mutagenesis, b) characterize the discovered genes by testing the created mutants in the established in vitro human epithelial cells (e.g. HEp-2 cells, polarized Caco-2 cells, and LS174T cells) and polarized human intestinal ex vivo organ culture models to study Salmonella virulence (bacterial adhesion, invasion, and ...

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Indirect evidence suggests that stimulation of α1-adrenergic receptors (ARs) increases smooth muscle cell (SMC) growth in the growing and adult artery and worsens atherosclerosis and restenosis after balloon injury. In support of a direct adrenergic effect, we have previously shown that α1D-AR stimulation induces SMC hypertrophy in cell and vessel organ culture. Because interactions between α1-ARs and peptide growth factors may be important in normal and pathological SMC growth, herein we examined regulation of α1D-AR expression by growth factors. Platelet-derived growth factor (PDGF)-BB dose- and time-dependently lowered α1D mRNA in cultured quiescent SMCs (e.g., 58% inhibition at 20 ng/ml, 24 h, p , .05), whereas other α1-AR transcripts were unaffected. This same selective effect was seen in the medial layer of aorta in ex vivo organ culture. However, PDGF-AA, insulin-like growth factor-1, insulin, epidermal growth factor, endothelin, histamine, and serotonin had no effect, whereas ...

After injury to the animal epidermis, a variety of genes are transcriptionally activated in nearby cells to regenerate the missing cells and facilitate barrier repair. The range and types of diffusible wound signals that are produced by damaged epidermis and function to activate repair genes during epidermal regeneration remains a subject of very active study in many animals. In Drosophila embryos, we have discovered that serine protease function is locally activated around wound sites, and is also required for localized activation of epidermal repair genes. The serine protease trypsin is sufficient to induce a striking global epidermal wound response without inflicting cell death or compromising the integrity of the epithelial barrier. We developed a trypsin wounding treatment as an amplification tool to more fully understand the changes in the Drosophila transcriptome that occur after epidermal injury. By comparing our array results with similar results on mammalian skin wounding we can see which

Schreml, Stephan, Meier, Robert Johannes, Kirschbaum, Michael, Kong, Su-Chii, Gehmert, Sebastian, Felthaus, Oliver, Küchler, Sarah, Sharpe, Justin R., Wöltje, Kerstin, Weiß, Katharina, Albert, Markus, Seidl, Uwe, Schröder, Josef, Morsczeck, Christian, Prantl, Lukas, Duschl, Claus, Pedersen, Stine F., Gosau, Martin, Breneburg, Mark, Wolfbeis, Otto S., Landthaler, Michael und Babilas, Philipp (2014) Luminescent Dual Sensors Reveal Extracellular pH-Gradients and Hypoxia on Chronic Wounds That Disrupt Epidermal Repair. Theranostics 4 (7), S. 721-735 ...

Schreml S, Meier RJ, Kirschbaum M, Kong SC, Gehmert S, Felthaus O, Küchler, S., Sharpe JR, Wöltje K, Weiß KT, Albert M, Seidl U, Schröder J, Morsczeck C, Prantl L, Duschl C, Pedersen SF, Gosau M, Berneburg M, Wolfbeis OS, Landthaler M, Babilas P.:Luminescent Dual Sensors Reveal Extracellular pH-Gradients and Hypoxia on Chronic Wounds That Disrupt Epidermal Repair. Theranostics, 4: 721-735 ...

All dressings come in premade peel packs, and can be simply applied in theatre directly to the wound bed. This will then be covered with an absorbent second layer dressing (allevyn) and tape (hypafix). Dressings will be removed at seven days to assess the wound, then reapplied if necessary every seven days until full re-epithelialisation ...

Local drug delivery by coronary stents is of current research interest. Organ culture of human vascular tissue is a model of intimal hyperplasia. We report an ex vivo organ culture model of stented vessels. This allows stent-artery interactions to be studied in living tissue. The recognized anti-restenosis agent paclitaxel was chosen to test the organ culture model. Mammary artery specimens were cultured closed (i.e. without opening them flat) for 72h. Phosphocholine-coated stents, half of them loaded with the anti-restenosis drug paclitaxel, were implanted. The absorption and elution characteristics of paclitaxel were established. Artery tissue remained viable at 72h when cultured closed, despite stent implantation. Specimens developed smooth muscle cell proliferation. The stents absorbed up to 127±29μg of paclitaxel, with a biphasic elution curve. A mean of 13% of the absorbed paclitaxel remained after a 24h perfusion. In mammary artery, these paclitaxel stents reduced or abolished smooth ...

Background and Objectives: We have previously demonstrated the efficacy of a non-invasive, non-contact, fast and simple but robust fluorescence imaging (u-FEI) method to monitor the healing of skin wounds in vitro. This system can image highly-proliferating cellular processes (295/340 nm excitation/emission wavelengths) to study epithelialization in a cultured wound model. The objective of the current work is to evaluate the suitability of u-FEI for monitoring wound re-epithelialization in vivo. Study Design: Full-thickness wounds were created in the tail of rats and imaged weekly using u-FEI at 295/340nm excitation/emission wavelengths. Histology was used to investigate the correlation between the spatial distribution and intensity of fluorescence and the extent of wound epithelialization. In addition, the expression of the nuclear protein Ki67 was used to confirm the association between the proliferation of keratinocyte cells and the intensity of fluorescence. Results: Keratinocytes forming ...

Owing to its multifunctionality, TGFβ is involved in nearly every aspect of wound healing ( OKane and Ferguson, 1997; Roberts and Sporn, 1996). It stimulates chemotaxis and proliferation, and modulates gene expression that is dependent upon cell type and differentiation. One important step during cutaneous wound healing is the re-establishment of the epithelial barrier. The role of TGFβ in the re-epithelialization process appears to be contradictory. On the one hand, TGFβ, which is upregulated in keratinocytes after wounding, is a strong inhibitor of keratinocyte proliferation ( Coffey et al., 1988; Sellheyer et al., 1993). This could interfere with the supply of keratinocytes from the wound edge needed to cover the wound bed. On the other hand, TGFβ induces the expression of integrins necessary for keratinocyte migration over the provisional fibronectin matrix ( Gailit et al., 1994; Zambruno et al., 1995). In most studies investigating the role of TGFβ in re-epithelialization, it was not ...

Gender is one of the factors influencing the intestinal microbial composition in mammals, but whether fish also have gender-specific intestinal microbial patterns remains unknown. In this decade, endocrine disrupting chemicals in surface and ground water of many areas and increasing observation of freshwater male fish displaying female sexual characteristics have been reported. Here we identified the difference in intestinal microbiota between male and female zebrafish, and revealed the influence of endocrine disrupting chemicals on zebrafish intestinal microbiota by using high-throughput sequencing. The results indicated that Fusobacteria, Bacteroidetes and Proteobacteria were dominant in the gut of zebrafish and there were no obvious gender-specific intestinal microbial patterns. Two endocrine disrupting chemicals, Estradiol (E2) and Bisphenol A (BPA), were selected to treat male zebrafish for 5 weeks. E2 and BPA increased vitellogenin expression in the liver of male zebrafish and altered the

The aortic valve functions in a complex mechanical environment which leads to force-dependent cellular and tissue responses. Characterization of these responses provides a fundamental understanding of valve pathogenesis. The aim of this work was to study the biological characteristics of native porc …

Detect Clara Cell Secretory Protein using this Anti-Clara Cell Secretory Protein Antibody validated for use in IH & WB. Find MSDS or SDS, a COA, data sheets and more information.

As we grow in age, there are so many changes in the body which can interfere with the natural healing process of wounds.. It is truly devastating to have wounds that take forever to heal. Ignoring a slow healing wound might increase the risk of serious infection and even amputation. Lets discover how placental extracts help to heal wound. Animal placental extracts offer complementary therapy and give renewed hope to people suffering from slow healing wounds and other related complications.. Naturally rich in bio-active nutrients, animal placental extracts from Sheep or Deer have the ability to promote faster wound healing and regenerate damaged cells. Placental extracts activates the natural release of HGH in the body, which then stimulate the renewal of damaged cells as well in repairing the wounded cells.. Placental extracts activate Fibroblasts cells within the skin dermis, these specialized skin cells are responsible for producing connective tissue, collagen, elastin and glycosaminoglycans. ...

It is often a protracted process: Wound and scar therapy require patience from patients and that they stick to their treatments. Regardless of whether the wound is the result of an accident or surgery - healing follows the same mechanisms. After blood clotting and after scabbing, a more or less severe inflammatory response occurs after approx. 48 hours have passed. Then the actual wound healing starts, which results in the formation of granulate tissue or a tissue bud in the lower connective tissue. The number of inflammatory cells drops and the increase of collagen and blood vessels can be observed. The re-epithelialization, i.e. the new formation of tissue covering the outer skin of the body is possible thanks to the stem cells in the epidermis.. Wound healing may take longer depending on the individual case, especially with older, multimorbid patients. Confocal laser scanning microscopy can facilitate monitoring even complex and problematic wounds and checking for epidermal regrowth. The ...

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TY - JOUR. T1 - Human lactoferrin stimulates skin keratinocyte function and wound re-epithelialization. AU - Tang, L.. AU - Wu, J. J.. AU - Ma, Q.. AU - Cui, T.. AU - Andreopoulos, F. M.. AU - Gil, J.. AU - Valdes, J.. AU - Davis, S. C.. AU - Li, J.. PY - 2010/7. Y1 - 2010/7. N2 - Background Human lactoferrin (hLF), a member of the transferrin family, is known for its antimicrobial and anti-inflammatory effects. Recent studies on various nonskin cell lines indicate that hLF may have a stimulatory effect on cell proliferation. Objectives To study the potential role of hLF in wound re-epithelialization. Materials and methods The effects of hLF on cell growth, migration, attachment and survival were assessed, with a rice-derived recombinant hLF (holo-rhLF), using proliferation analysis, scratch migration assay, calcein-AM/propidium iodide staining and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) method, respectively. The mechanisms of hLF on cell proliferation and ...

Be required if vision deteriorates when tapering begins. Tumors of or resembling sweat glands Controllde sweat glands are represented in the eyelids by Mollвs glands; eccrine sweat glands are present in the lids both at the lid margin and in icpro dermis over the surface of the eyelid.

The main concerns I had were sunspots and pore size. I knew from my 20+ years in cosmetic medicine that these two issues are notoriously difficult to treat, but I was seeing the Parker Rejuvenation Lift noticeably reduce the appearance of spots and reducing pore size, so I decided to go for it.. Before my treatment, a topical numbing cream was applied to my face to make sure I was totally comfortable and it definitely worked! I didnt experience any discomfort whatsoever during my treatment with Halo.. For the first hour after treatment, I did feel like I had an intense sunburn on my face and my skin was red. The redness went away after about a day, and I was surprised by how quickly it subsided compared to what you see with other lasers. To care for my skin in the days after treatment, I applied an epidermal repair cream on my face.. ...

Bisphenol F (BPF) has been frequently detected in various environmental compartments, and previous studies found that BPF exhibits similar estrogenic and anti-androgenic effects on the mammalian endocrine system to those of bisphenol A (BPA). However, the potential disrupting effects of BPF on aquatic organisms and the underling disrupting mechanisms have not been investigated. In this study, the potential disrupting mechanisms of BPF on the hypothalamic-pituitary-gonadal (HPG) axis and liver were probed by employing the OECD 21-day short-term fecundity assay in zebrafish. The results show that BPF exposure (1 mg/L) impaired the reproductive function of zebrafish, as exemplified by alterations to testicular and ovarian histology of the treated zebrafish. Homogenate testosterone (T) levels in male zebrafish decreased in a concentration-dependent manner, and 17β-estradiol (E2) levels increased significantly when fish were exposed to 0.1 and 1 mg/L BPF. The real-time polymerase chain reaction was ...

Previously, it has been reported that corneal epithelial damage, even without stromal involvement, elicits three general phases of healing28 characterized by an initial lag phase lasting for 6 hours, followed by epithelial (basal and suprabasal) migration and proliferation.29-31 In this specific murine model, corneal epithelial injury evokes two waves of neutrophil migration initiated from the limbal vessels within 6 hours and peaking at 12 to 18 hours and 30 to 36 hours, respectively.12 It has also been shown γδ T cells are necessary for the localization of these neutrophil and together promote efficient epithelial repair by promoting cytotoxic effects on activated macrophage.32,33 Hence, we speculate that one plausible mechanism for AMUC to promote faster epithelialization is through anti-inflammatory actions. This is supported by the finding that CD45+ (inflammatory) cells were significantly decreased in the AMUC group. Additionally, the finding that AMs therapeutic efficacy can be ...

BackgroundIngestion of the lectins present in certain improperly cooked vegetables can result in acute GI tract distress, but the mechanism of toxicity is unknown. In vivo, gut epithelial cells are constantly exposed to mechanical and other stresses and consequently individual cells frequently experience plasma membrane disruptions. Repair of these cell surface disruptions allows the wounded cell to survive: failure results in necrotic cell death. Plasma membrane repair is mediated, in part, by an exocytotic event that adds a patch of internal membrane to the defect site. Lectins are known to inhibit exocytosis. We therefore tested the novel hypothesis that lectin toxicity is due to an inhibitory effect on plasma membrane repair.Methods and FindingsRepair of plasma membrane disruptions and exocytosis of mucus was assessed after treatment of cultured cell models and excised segments of the GI tract with lectins. Plasma membrane disruptions were produced by focal irradiation of individual cells, using a

The mammalian heart has generally been considered to lack the ability to repair itself after injury, but a 2011 study in new-born mice challenged this view, providing evidence for complete regeneration after resection of 10% of the apex. However, in this study researchers attempted to replicate these recent findings but failed to uncover any evidence of complete heart regeneration in new-born mice that underwent apex resection.

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6yrs: not recommended. Do not exceed recommended dose. ≥6yrs: 1 drop every 2hrs while awake; max 9 drops/day. After reepithelialization, 1 drop every 4hrs for 7 days (at least 5 drops/day); max 21 days therapy. Reevaluate if no improvement in 7 days or incomplete reepithelialization in 14 days.. ...

Spinal tissue distraction devices that include at least one guide member for guiding a distraction device to a location between layers of spinal tissue. The guide member has a proximal end portion and a distal end portion. The distal end portion is adapted for insertion between the tissue layers and defines a shape of a desired support structure. The distraction device is slidably mounted on the guide member for advancing the distraction device distally along the guide member for insertion of the distraction device between the tissue layers. The distraction device substantially conforms to the shape defined by the distal end portion of the guide member to form a support structure that separates, supports or both separates and supports the tissue layers.

Here we selected eight genes including FOLR3, CDH6, CXCL12, NPPB, cyclin D2, IGF2, KRT8, and CRABP2, for verifying results from the chip data because these genes showed gradual and drastic changes in expression patterns. It has not been reported to date that these selected genes are directly related to cellular senescence. Although an interest in IGF2 derives mostly from its connection with IGF1 and how the IGF1/GH axis appears to regulate aging in model organisms, IGF2s role in aging is unproven (17). Recently, it was reported that overall IGF2 expression increased during senescence of human prostate epithelial cells due to the loss of IGF2 imprinting (18). However, our RT-qPCR result, which did not match our chip data, showed that IGF2 mRNA levels were increased by the 6th passage and then abruptly dropped by the 10th passage (Fig. 4). The discrepancy between our result and theirs might be caused by our using different cell types. Or, IGF2 might not be a main regulator of cellular senescence. ...

Looking for online definition of Reepithelialization in the Medical Dictionary? Reepithelialization explanation free. What is Reepithelialization? Meaning of Reepithelialization medical term. What does Reepithelialization mean?

Background - Burns is a complex condition requiring assessment and addressing of both the wound and the patient in a holistic way. In spite of tremendous improvements in burn care, infection continues to remain an important cause of morbidity and mortality. Human Β Defensins (HBD) are a group of recently discovered antimicrobial peptides. The main subtypes include HBD1, 2 and 3 and are individually known to have other functions apart from being anti-microbial. Some of these are inherently expressed while others are induced in response to microbial challenge. Aims - The aim of the current PhD was to understand the pattern of expression of HBDs in acute burns, their source of expression, and factors influencing the expression, with a view to use these peptides as therapeutic agents in future. Methods - The expression of HBD1, 2 & 3 was determined at mRNA and protein levels in acute burn wounds of different burn durations, using real time rt-PCR and immunohistochemistry, respectively. The ...

Injury to stratified epithelia causes a strong induction of keratins 6 (K6) and 16 (K16) in post-mitotic keratinocytes located at the wound edge. We show that induction of K6 and K16 occurs within 6 h after injury to human epidermis. Their subsequent accumulation in keratinocytes correlates with the profound reorganization of keratin filaments from a pan-cytoplasmic distribution to one in which filaments are aggregated in a juxtanuclear location, opposite to the direction of cell migration. This filament reorganization coincides with additional cytoarchitectural changes and the onset of re-epithelialization after 18 h post-injury. By following the assembly of K6 and K16 in vitro and in cultured cells, we find that relative to K5 and K14, a well-characterized keratin pair that is constitutively expressed in epidermis, K6 and K16 polymerize into short 10-nm filaments that accumulate near the nucleus, a property arising from K16. Forced expression of human K16 in skin keratinocytes of transgenic ...

Expression of growth factors in re-epithelialization of diabetic foot ulcers after treatment with non-thermal plasma radiation, Neda Naderi, Mohammad Zaefizadeh

Purpose. The reepithelialization of the corneal surface is an important process for restoring the imaging properties of this tissue. The purpose of the present study was to characterize and validate a new human in vitro three-dimensional corneal wound healing model by studying the expression of basement membrane components and integrin subunits that play important roles during epithelial cell migration and to verify whether the presence of exogenous factors could accelerate the reepithelialization. Methods. Tissue-engineered human cornea was wounded with a 6-mm biopsy punch, and the reepithelialization from the surrounding margins was studied. Biopsy samples of the reepithelialized surface were harvested 3 days after wounding and were processed for histologic, electron microscopic, and immunofluorescence analyses. The effects of fibrin and epithelial growth factor (EGF) on wound reepithelialization were also studied. Results. Results demonstrated that this in vitro model allowed the migration of ...

3.0.CO;2-J. PMID 9924435. Schrementi ME, Ferreira AM, Zender C, DiPietro LA (2008). Site-specific production of TGF-beta in oral mucosal and cutaneous wounds. Wound Repair Regen. 16 (1): 80-6. doi:10.1111/j.1524-475X.2007.00320.x. PMID 18086295. Frank S, Stallmeyer B, Kämpfer H, Kolb N, Pfeilschifter J (August 2000). Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair. J. Clin. Invest. 106 (4): 501-9. doi:10.1172/JCI9148. PMC 380250 . PMID 10953025. Gröschl M, Topf HG, Kratzsch J, Dötsch J, Rascher W, Rauh M (April 2005). Salivary leptin induces increased expression of growth factors in oral keratinocytes. J. Mol. Endocrinol. 34 (2): 353-66. doi:10.1677/jme.1.01658. PMID 15821102. Costigan DC, Guyda HJ, Posner BI (May 1988). Free insulin-like growth factor I (IGF-I) and IGF-II in human saliva. J. Clin. Endocrinol. Metab. 66 (5): 1014-8. doi:10.1210/jcem-66-5-1014. PMID 3360895. Todorović V, Pesko P, Micev M, et al. (October 2008). ...

Musashi-1, a neural RNA-binding protein, is important for maintaining neural stem cells. Both Musashi-1 and Hes1, a transcriptional factor regulated by Musashi-1, are expressed in the small intestine. Here we show that Musashi-1 is present in a few epithelial cells just above the Paneth cells in the …

The important pro-scarring characteristics of AMP and the lack of these in UPD processes have led to research on using these peptides to treat chronic wounds. In vivo studies using adenoviral transfer of LL-37 to wounds of diabetic ob/ob mice, showed a significant increase in the production of LL-37 in the skin of these mice, improving re-epithelialization and the formation of granular tissue, decreasing the time healing compared to untreated mice.. This was later confirmed in an independent study, where LL-37 was shown to promote both re-epithelialization and angiogenesis in both in vitro and in vivo models of chronic wounds.. Subsequently, using LL-37 and PDH IDR-1018, which is a synthetic derivative of bovine bactenecin, IDR-1018 was shown to be less toxic than LL-37 in vitro. Furthermore, it is shown that healing was accelerated in wounds infected by S. aureus in both murine and porcine models.. However, when using this peptide in murine models of diabetes, there was no difference between ...

A scar is an area of fibrous tissue that replaces normal skin after an injury. Scars result from the biological process of wound repair in the skin, as well as in other organs and tissues of the body. Thus, scarring is a natural part of the healing process. With the exception of very minor lesions, every wound (e.g., after accident, disease, or surgery) results in some degree of scarring. An exception to this are animals with complete regeneration, which regrow tissue without scar formation. Scar tissue is composed of the same protein (collagen) as the tissue that it replaces, but the fiber composition of the protein is different; instead of a random basketweave formation of the collagen fibers found in normal tissue, in fibrosis the collagen cross-links and forms a pronounced alignment in a single direction. This collagen scar tissue alignment is usually of inferior functional quality to the normal collagen randomised alignment. For example, scars in the skin are less resistant to ultraviolet ...

PHENOTYPE: Mice homozygous for a knock-out allele develop normally but display enhanced keratinocyte migration and accelerated re-epithelialization during cutaneous wound healing. [provided by MGI curators ...

Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling (By similarity ...

Not one more penny should be spent on the $6.35 million water treatment plant project until access to the stadium well has been obtained from Phillips Exeter Academy. Town Manager Russell Dean agreed to negotiate with PEA for this access and he failed to do so. Now, with only two out of three wells available for this project, it is likely no longer viable. Deans failure to act has placed the town at the mercy of PEA. Who knows what PEA will want for compensation? How much can ratepayers afford? They will ultimately pay the bill.. To the Editor ...

Scientists hailing from the Wake Forest Institute for Regenerative Medicine (WFIRM) have been putting their efforts into a revolutionary approach for helping chronic kidney disease sufferers get a firmer grasp on their situation and repair any tissues that may have been ravaged by the disease. This solution comes in the form of human amniotic fluid-derived stem cells, which the scientists have posited would not only repair tissue, but even help recover kidney function that has been lost. The researchers at WFIRM have been at the cutting edge of stem cell research for quite some time, with their teams also pioneering the equally impressive 3D bioprinting of organs and tissues. According to the scientists, the amniotic fluid-derived stem cells are unique in that they can be utilized as universal cell sources, meaning they can fill in for numerous different types of cells, while simultaneously benefiting the entirety of the organism with anti-inflammatory properties and potential ...

One aspect of the present invention is a connective tissue distraction device comprising: a first transmitting structure for transmitting force to a first tissue region and a second transmitting structure for transmitting force to a second tissue region by contact with bone, and an expansion device for exerting force distracting the first transmitting structure from the second transmitting structure. At least one of the first transmitting device, the second transmitting device and the expansion structure comprises in whole or in part a biodegradable, bioerodible or bioresorbable material.

Regenerative medicine and tissue engineering solutions of heavily innervated tissues are at this point lacklustre. This thesis expands our knowledge of appropriate acellular scaffolds for tissue repair in general and nerve regeneration in particular. The optimal surgical procedure for the implantation of artificial extracellular matrix (ECM) was evaluated for recombinant human collagen (RHCIII) implants. Suturing techniques, as well as the usage of human amniotic membrane bandages were evaluated. While complete regeneration of corneal tissues occurred, only slight differences in effects of surgical technique could be found.. The safety and efficacy of clinical trials using mesenchymal stromal cells (MSCs) was evaluated by conducting a systematic review and meta-analysis. MSC therapy was shown to be safe, with no increases mortality, rehospitalization or adverse events. There was also an indication of efficacy, as the overall mortality in the studies included was significantly smaller in the ...

Understanding how probiotics, prebiotics or postbiotics work in preclinical models that resemble the human situation can allow a rational choice of the different strains or compounds for clinical and/or commercial use according to the pathological condition to which they are targeted. Indeed, we recently demonstrated that different Lactobacilli species can be classified as either immunogenic or tolerogenic.17 Testing of different probiotics has often been performed on isolated immune cells or peripheral blood mononuclear cells.21 However, particularly in the gut, the interaction of bacteria with the mucosa is an unusually complex event requiring attachment or degradation of mucus, competition with the microbiota and resistance to antimicrobial peptides.13 In addition, the function of immune cells is influenced by the local microenvironment and this is required to preserve intestinal homeostasis.4-22 Indeed, we have shown that signals from intestinal epithelial cells can drive the ...

An apparatus and method for distracting, in a given direction, and supporting two tissue surfaces is provided. A plurality of wafers are consecutively inserted using a wafer insertion apparatus between the two tissue surfaces to create a column of wafers. A detachable wafer assembly is provided that includes a base wafer initially associated with a track assembly of a wafer insertion apparatus. The base wafer is dislodged from the track assembly so that the base wafer is left within the distraction site as the track assembly is removed. A top cap wafer is provided that is situated at the top of the wafer stack, in which the top cap wafer is larger than the remaining wafers to form a gap surrounding the stack to receive biologic material.

An apparatus and method is provided for interbody fusion including distracting, in a given direction, and supporting opposing vertebral bodies. A plurality of wafers are consecutively inserted between the vertebral bodies to create a column of wafers. The column of wafers is oriented between the vertebral bodies so as to expand in the given direction as the wafers are consecutively added to the column.

The wound healing attributes of five acellular dermal skin substitutes were compared, in a two-step procedure, in a porcine model. Ten pigs were included in this experimental and randomized study. During the first step, dermal substitutes (Integra(®

TY - JOUR. T1 - Ethanol alters the osteogenic differentiation of amniotic fluid-derived stem cells. AU - Hipp, Jennifer A.. AU - Hipp, Jason D.. AU - Atala, Anthony. AU - Soker, Shay. PY - 2010/10/1. Y1 - 2010/10/1. N2 - Background: Fetal alcohol spectrum disorder (FASD) is a set of developmental defects caused by prenatal alcohol exposure. Clinical manifestations of FASD are highly variable and include mental retardation and developmental defects of the heart, kidney, muscle, skeleton, and craniofacial structures. Specific effects of ethanol on fetal cells include induction of apoptosis as well as inhibition of proliferation, differentiation, and migration. This complex set of responses suggests that a bioinformatics approach could clarify some of the pathways involved in these responses. Methods: In this study, the responses of fetal stem cells derived from the amniotic fluid (AFSCs) to treatment with ethanol have been examined. Large-scale transcriptome analysis of ethanol-treated AFSCs ...

lymphomas), and endocrine diseases. The eccrine glands are found all over the body, with the exception of the lips and around parts of the external genitalia. The eccrine sweat glands are located all over your body and produce a lightweight, odorless sweat. In contrast, apocrine glands pinch off a portion of the cell with substances. 16 It tends to occur in the middle age to elderly population and does not appear to have a predilection for race or sex. Other components include antimicrobial peptides (e.g. Pilosebaceous unit with apocrine and eccrine sweat glands. Based on data from ref. The eccrine glands, however, do not reach as far into the dermis and secrete their fluids directly onto the skins surface via the pores. This can be a major inconvenience, but it is not associated with morphologic or functional abnormalities of the glands, nor with internal diseases . The sweat of apocrine sweat glands is more odorous that the sweat produced by eccrine sweat glands. The eccrine glands secretory ...

Difference between Apocrine and Eccrine sweat glands Apocrine vs Eccrine sweat glands The skin is the largest organ of the human body. It consists of various structures like the sweat glands, hair follicles, blood vessels, nerve endings and the three layers- epidermis, dermis and hypodermis. One might be surprised to see that so many structures are present, perfectly organized in the thin […]

In the paediatric population, pain and distress associated with burn injuries during wound care procedures remain a constant challenge. Although silver dressings are the gold standard for burn care in Australasia, very few high-level trials have been conducted that compare silver dressings to determine which will provide the best level of care clinically. Therefore, for paediatric patients in particular, identifying silver dressings that are associated with lower levels of pain and rapid wound re-epithelialisation is imperative. This study will determine whether there is a difference in time to re-epithelialisation and pain and distress experienced during wound care procedures among Acticoat™, Acticoat™ combined with Mepitel™ and Mepilex Ag™ dressings for acute, paediatric partial thickness burns. Children aged 0 to 15 years with an acute partial thickness (superficial partial to deep partial thickness inclusive) burn injury and a burn total body surface area of ≤10% will be eligible for the

Clara cells are non-mucous and non-ciliated secretory cells found in the primary bronchioles of the lungs, and were originally described by their namesake, Max Clara in 1937.. Clara cells are dome-shaped and have short microvilli. One of the main functions of Clara cells is to protect the bronchiolar epithelium. They do this by secreting a small variety of products, including Clara cell secretory protein (CCSP) and a component of the lung surfactant. They are also responsible for detoxifying harmful substances inhaled into the lungs. Clara cells accomplish this with cytochrome P450 enzymes found in their smooth endoplasmic reticulum. Clara cells also multiply and differentiate into ciliated cells to regenerate the bronchiolar epithelium.. CC10 (Clara cell secretory protein) ...

Results Compared to control lungs, protein levels of class I (HDAC1, HDAC2, HDAC3, HDAC8) and class II HDACs (HDAC4, HDAC 5, HDAC 7, HDAC 9) were significantly elevated in IPF lungs. Using immunohistochemistry, strong induction of nearly all HDAC enzymes was observed in myofibroblasts of fibroblast foci and in abnormal bronchiolar basal cells at sites of aberrant re-epithelialisation in IPF lungs, but not in controls. Treatment of primary IPF fibroblasts with the pan-HDACi LBH589 resulted in significantly reduced expression of genes associated with ECM synthesis, proliferation and cell survival, as well as in suppression of HDAC7, and was paralleled by induction of endoplasmic reticulum stress and apoptosis. The profibrotic and apoptosis-resistant phenotype of IPF fibroblasts was also partly attenuated by the class I HDACi VPA. ...

The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on |i|in vitro|/i| models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment |i|in vivo|/i| using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days

Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix...

TY - JOUR. T1 - Thyroid hormone-upregulated expression of Musashi-1 is specific for progenitor cells of the adult epithelium during amphibian gastrointestinal remodeling. AU - Ishizuya-Oka, Atsuko. AU - Shimizu, Katsuhiko. AU - Sakakibara, Shi Ichi. AU - Okano, Hideyuki. AU - Ueda, Shuichi. PY - 2003/8/1. Y1 - 2003/8/1. N2 - In the amphibian gastrointestine during metamorphosis, the primary (larval) epithelium undergoes apoptosis. By contrast, a small number of undifferentiated cells including stem cells actively proliferate and differentiate into the secondary (adult) epithelium that resembles the mammalian counterpart. In the present study, to clarify whether Musashi-1 (Msi-1), an RNA-binding protein, serves as a marker for progenitor cells of the adult epithelium, we chronologically examined Msi-1 expression in the Xenopus laevis gastrointestine by using in situ hybridization and immunohistochemistry. Similar expression profiles of Msi-1 were observed at both mRNA and protein levels. In both ...

TY - JOUR. T1 - Thyroid hormone-upregulated expression of Musashi-1 is specific for progenitor cells of the adult epithelium during amphibian gastrointestinal remodeling. AU - Ishizuya-Oka, Atsuko. AU - Shimizu, Katsuhiko. AU - Sakakibara, Shi Ichi. AU - Okano, Hideyuki. AU - Ueda, Shuichi. PY - 2003/8/1. Y1 - 2003/8/1. N2 - In the amphibian gastrointestine during metamorphosis, the primary (larval) epithelium undergoes apoptosis. By contrast, a small number of undifferentiated cells including stem cells actively proliferate and differentiate into the secondary (adult) epithelium that resembles the mammalian counterpart. In the present study, to clarify whether Musashi-1 (Msi-1), an RNA-binding protein, serves as a marker for progenitor cells of the adult epithelium, we chronologically examined Msi-1 expression in the Xenopus laevis gastrointestine by using in situ hybridization and immunohistochemistry. Similar expression profiles of Msi-1 were observed at both mRNA and protein levels. In both ...

Using microfabricated technology, the team found that layers of human outer skin cells, known as keratinocytes, are able to form suspended multicellular bridges over regions devoid of extracellular matrix support. Migrating keratinocytes are able to move forward as a united and homogenous collection of cells to form a protective barrier over a wounded area. Eventually, these cells come together to form suspended bridges over regions which are not conducive for cell adhesion. It was previously not understood how this healing process, known as re-epithelialization, could occur over a wound bed that did not provide a homogeneous coating of extracellular matrix for cells to migrate on.. The researchers also found out that the suspended cell sheet is created through the build-up of large-scale tension activated by acto-myosin, a kind of motor protein that can cause contraction in cells. They found the cell sheet to be elastic-like in behavior, which partly explained its ability to form ...

Before the rapid development of pharmaceuticals, ancient traditional remedies extracted from sources of nature served as the cure to many ailments. Today, Wound healing remains as a challenging clinical problem often leading to complications that result in morbidity. As an effort to reduce this burden, the focus of wound care has shifted towards grasping a better understanding of the underlying physiology as well as the development of new therapeutic approaches to manage acute and long term wound care.. The first ingredient in WoundKreme is honey. Honey is a biological wound dressing with multiple bioactivities that has been used for many centuries now. It provides a moist healing environment which aids to rapidly clear infection, reduce inflammation, edema, and exudation in the wound. It also increases the rate of healing by stimulating the angiogenesis process, granulation of tissue, and re-epithelialization. The combined anti-bacterial and anti-inflammatory properties of honey explains why it ...

TY - JOUR. T1 - Immunohistochemical analysis revealed a correlation between musashi-2 and cyclin-d1 expression in patients with oral squamous cells carcinoma. AU - Troiano, Giuseppe. AU - Caponio, Vito Carlo Alberto. AU - Botti, Gerardo. AU - Aquino, Gabriella. AU - Losito, Nunzia Simona. AU - Pedicillo, Maria Carmela. AU - Zhurakivska, Khrystyna. AU - Arena, Claudia. AU - Ciavarella, Domenico. AU - Mastrangelo, Filiberto. AU - Russo, Lucio Lo. AU - Muzio, Lorenzo Lo. AU - Pannone, Giuseppe. PY - 2020/1/1. Y1 - 2020/1/1. N2 - Aim: Musashi 2 (MSI2), which is an RNA-binding protein, plays a fundamental role in the oncogenesis of several cancers. The aim of this study is to investigate the expression of MSI2 in Oral Squamous Cell Carcinoma (OSCC) and evaluate its correlation to clinic-pathological variables and prognosis. Materials and Methods: A bioinformatic analysis was performed on data downloaded from The Cancer Genome Atlas (TCGA) database. The MSI2 expression data were analysed for their ...

Application of Artificial Dermis to Periodontal Therapy. Epithelial Regeneration Responses After Collagen Matrix Implantation into Defects of the Palatal Gingiva in Rats.:Epithelial Regeneration Responses After Collagen Matrix Implantation into Defects of the Palatal Gingiva in Rats （1994 ...

Comprised of a 17-bed inpatient unit and the Acute Burn and Wound Clinic, the Burn Center occupies a 16,000-square-foot space on our downtown Minneapolis campus. Our highly trained, multidisciplinary team works closely with patients and families in a supportive, state-of-the-art environment to meet their unique needs and achieve the highest level of functioning. Treating an average of 10 inpatients per day and 20+ patients per day in the clinic, HCMCs Burn Center is one of the busiest centers in the nation - and also one of the finest. It is verified by the American Burn Association and the American College of Surgeons.. ...

Oral cancer (OC) patients are at high risk to develop recurrent disease or secondary primary cancers with no available biomarkers to detect these events until a