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BioAssay record AID 607075 submitted by ChEMBL: Induction of Myod1 mRNA expression in Wistar rat at 60 mg/kg, po qd for 24 days by RT-PCR analysis relative to control.
2 -methylbut-2-ene (2M2B) has a low order of acute toxicity in animals by the dermal and inhalation routes of exposure. It is classifiable as harmful by the oral route of exposure. Non-human data Oral In order to determine the acute oral LD50 of 2M2B a range-finding and definitive study have been performed in albino Wistar rats. In the definitive study, groups of six males and six females were dosed with 1.0, 1.6, 2.5, 4.0, 6.3 and 10 ml/kg. The majority of deaths occurred within the first 3 days following dosing and most survivors had recovered from signs of intoxication by the third day. The author estimated the oral LD50 to be in the range of 1 to 4 ml/kg (i.e., 700 to 2600 mg/kg) (SRC, 1980). Further inspection of the data (OECD SIDS, 2005) allows this to be refined to a range of 1.6 to 2.5 mL/kg (1000 to 1700 mg/kg). Dermal In order to determine the acute dermal LD50 of 2M2B range-finding and definitive studies have been performed in albino Wistar rats. The acute dermal LD50 value of 2M2B ...
seeds on male Wistar albino rats was investigated. The proteins were found to have high true digestibility (TD) and feed conversion ratio (FCR). The net protein retention (NPR), protein efficiency ratio (PER), protein retention efficiency (PRE), Net protein value (NPV), Nitrogen efficiency ratio (NER), feed efficiency ratio (FER) and relative NPR (RNPR) of the ungerminated seeds were significantly lower ( ...
Materials And Methods: A total of 30 male Wistar albino rats weighing 250-280 g were used in this study. In addition, 2-cm-long skin incisions were created over bilaterally exposed skin of the tibia region in each rat. VEGF plasmid 2 μg was administered locally into the right side wound bed of each animal. No other procedure besides skin closure was administered on the left side. To determine histologic assessments, skin samples were obtained from six anesthetized rats at each interval (4, 8, 12, 16 and 30 days) through excisional biopsy. The tissues were fixed in 10% neutral-buffered formalin for 1 week and then embedded in paraffin wax. Transverse sections of the embedded tissue 5-7 μm thick were stained with hematoxylin and eosin (H and E ...
MATERIAL AND METHOD This study was performed on two 6-week male Albino Wistar rats (E1 and E2) bought from Iranian Pasteur Institute. Both experiments were kept in same animal house with a non-enriched environment, 12 hours light/12 hours dark cycle, standard food regimen and water ad libitum. After 18 weeks of keeping, the 24-week experiments were anesthetized by chloroform inhalation and killed with cervical dislocation. The skulls were dissected and cerebella were taken. To fix them, they were emerged in Clark solution for 48 hours. After fixation, the left hemispheres of each of two cerebella were separated from reminder at their junction with relevant vermises. The hemispheres were embedded in paraffin and 6µm parasagittal serial sections were prepared from each of them. For a simple random selection, every 3-5 sections of each hemisphere were mounted on a glass slide. Consequently, a lateral-medial series of slides contained the parasagittal serial sections were provided for each ...
BioAssay record AID 772436 submitted by ChEMBL: Hepatotoxicity in Triton-X-100-induced hyperlipidemia Wistar albino rat model assessed as SGPT level in serum at 250 mg/kg, po qd for 7 days.
Purpose: We have previously shown that delayed injection of bone marrow stem cell conditioned media (BMSC CM), 24 h after ischemia, rescued ischemic retinal functional and histological properties. We hypothesized that preconditioning the BMSC CM by exposure of the BMSCs to hypoxic conditions will increase the efficacy of this protection. In this study we examined the functional and histological outcomes, and the possible mechanisms of this neuroprotection.. Methods: Retinal ischemia was produced in adult Wistar rats by increasing IOP for 55 min. BMSCs which were harvested from male Wistar rat femurs were cultured and characterized. The cultured BMSCs produced hypoxic conditioned media, by exposure to hypoxia (1% O2) for 72 h, which was injected into the vitreous 24 h after the end of ischemia. Recovery was assessed one week later using electroretinography (ERG). Scotopic a- and b waves, oscillatory potentials (OPs) and P2 were measured. We also examined 4 micron thick paraffin-embedded sections ...
In this study, we investigate Nitric oxide synthase (NOS) expressions in adriamycin (ADR)-induced cadiomyopathy in rats. Sixty male Wistar rats were randomly divided into two main groups: control and ADR groups. Myocardial histopathological observation was performed; Expressions of 3 isoforms of NOS genes were examined by RT-PCR analysis; Expressions of 3 isoforms of NOS protein was assessed by Western blot analysis. Myocardium exhibited intensive morphological changes after 8 weeks of ADR treatment. The expression levels of inducible NOS (iNOS) gene and protein were significantly increased in ADRtreated rats after 8 weeks of treatment and then slightly increased at weeks 9 and 10 ...
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Rat Wistar Plasma from Innovative Research is manufactured using whole blood collections from Wistar rats. This product is manufactured in large batches, and is not available with any special instructions - if you require custom-collected material, please see our Innovative Grade US Origin Rat Wistar Plasma. Download S
Summary The aim of this study was to assess the possible effects of Sa 190 on male and female fertility and embryofetal development after repeated dose administrationin Wistar rats. The test item was administered daily in graduated doses to 3 groups of test animals, one dose level per group for a treatment period of 63 days, i.e. during 14 days of pre-mating and maximum 14 days of mating in both males and females, during the gestation period and up to post-natal day 12 in females. Males were dosed after the mating period until the minimum total dosing period of 28 days were completed. Animals of an additional control group were handled identically as the dose groups but received corn oil, the vehicle used in this study. The 4 groups comprised 10 male and 10 female Wistar rats. Before dosing all females were screened for two weeks for regular estrous cyclicity and animals (10 females/ group) with regular estorus cycle (4-5 day cycle) were used in the study. The following doses were evaluated: ...
Furthermore, the examination of DNA-bound FOXO3a by electrophoretic mobility-shift assay demonstrated a marked increase in nuclear, DNA bound FOXO3a in GK rats with MI compared to Sham-GK and corresponding post-MI Wistar controls (Figure 5D). ...
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A study to evaluate efficacy of Tinospora cordifolia (Guduchi) as analgesic agent using albino wistar rats as an experimental animal model
Twelve 4-week-old male Wistar rats weighing 100 g were fed diets semi-ad libitum for 22 d containing either 1.5% conjugated linoleic acid (CLA-diet) or high oleic sunflower oil (Control-diet). The CLA was structured triacylglycerol with predominantly cis-9, trans-11 and trans-10, cis-12 fatty acid isomers in the inner position and oleic acid in the other positions of the glycerol molecule. The rats were kept individually in metabolic cages. From days 8-16 energy, nitrogen (N) and carbon ...
Background: The use of Jatropha curcas leaves in folklore medicine has gained popularity in recent times due to its medicinal value but without regards to its adverse health effect.. Aim: This study aimed at investigating the effect of J. curcas leaves on the fertility of male Wistar rats.. Methodology: Healthy leaves of J. curcas were harvested, dried and extracted using soxhlet apparatus and ethanol as the solvent. Toxicity test was carried out using standard method. Twenty-four adult male Wistar rats were randomly divided into four groups of six each after seven days acclimatization period. Animals in group 1 were not treated, while those in groups 2, 3 and 4 were administered 100, 200 and 400 mg/kg body weight of J. curcas extracts respectively for twenty-eight days. Administration was done 12 hourly via oral route. At the end of the administration period, the rats were sacrificed after an overnight fast under diethyl ether as anesthesia. Blood samples were collected via cardiac puncture. ...
The present investigation was aimed at evaluating the wound healing potential of aerial parts and roots of Cleome viscosa Linn on albino Wistar rats. Excision and incision models were employed. In each wound model, male albino Wistar rats weighing 180-200g were divided into four groups of 6 animals each. In both the models, group I served as control and group II as reference standard treated with Soframycin. In an excision wound model, group III animals were treated with methanol extract of the aerial parts of C.viscosa (CVAMExt) 500 mg kg/ kg b.w and group IV animals were treated with methanol extract of the roots of C.viscosa (CVRMExt) 500 mg/ kg b.w, for 16 days respectively. In incision wound model, group III and IV animals were treated with CVAMExt and CVRMExt 500 mg/ kg b.w, for 9 days respectively. CVAMExt showed a 91.53% contraction in excision wounds, which was close to the contraction value of the reference drug Soframycin (100%). On the other hand, the same extract used on the incision wound
Abstract Aloe vera has been used since ages to manage a number of diseases causing pain, inflammation and fever. However, its effectiveness has not been scientifically certified. NSAIDs & Opioid are used continuously for the pain management. Alternative analgesics are needed to escape from the serious adverse effects of NSAIDs. Our study demonstrated significant analgesic activity of Aloe vera at doses of 200 mg/kg and 500 mg/kg. Materials and Methods: The study was carried out using Wistar Albino rats (150-200gm). Soxhlets apparatus was used to prepare the aqueous extract of Aloe vera [AEAV]. The animals were divided into 4 groups (n=6) receiving different treatments. Hot plate method, acetic acid induced writhing method and tail flick tests were used. The results were subjected to statistical analysis by using one way ANOVA followed by Dunnetts test. Results Analgesic effect of high dose of AEAV at 500 mg/kg was similar with the standard drug indomethacin. The extract (200 & 500 mg/kg) ...
The present study was designed to investigate the cardioprotective effects of Sundarban honey (SH) in rats with isoproterenol- (ISO-) induced myocardial infarction. Adult male Wistar Albino rats were pretreated with Sundarban honey (5 g/kg) daily for a period of 6 weeks. After the treatment period, ISO (85 mg/kg) was subcutaneously injected into the rats at 24 h intervals for 2 days. ISO-induced myocardial damage was indicated by increased serum cardiac specific troponin I levels and cardiac marker enzyme activities including creatine kinase-MB, lactate dehydrogenase, aspartate transaminase, and alanine transaminase. Significant increases in serum total cholesterol, triglycerides, and low-density lipoprotein-cholesterol levels were also observed, along with a reduction in the serum high-density lipoprotein-cholesterol level. In addition to these diagnostic markers, the levels of lipid peroxide products were significantly increased. The activities of antioxidant enzymes such as superoxide ...
The present study was designed to investigate the cardioprotective effects of Sundarban honey (SH) in rats with isoproterenol- (ISO-) induced myocardial infarction. Adult male Wistar Albino rats were pretreated with Sundarban honey (5 g/kg) daily for a period of 6 weeks. After the treatment period, ISO (85 mg/kg) was subcutaneously injected into the rats at 24 h intervals for 2 days. ISO-induced myocardial damage was indicated by increased serum cardiac specific troponin I levels and cardiac marker enzyme activities including creatine kinase-MB, lactate dehydrogenase, aspartate transaminase, and alanine transaminase. Significant increases in serum total cholesterol, triglycerides, and low-density lipoprotein-cholesterol levels were also observed, along with a reduction in the serum high-density lipoprotein-cholesterol level. In addition to these diagnostic markers, the levels of lipid peroxide products were significantly increased. The activities of antioxidant enzymes such as superoxide ...
Material and methods: Eighty adult male Wistar albino rats were divided into 5 groups. Group I (control group) contained 20 rats, whereas each of the other groups contained 15 rats. These experimental groups were cisplatin group (group II) which received saline injection 5 days prior and after a single cisplatin injection (7 mg/kg IP), cisplatin recovery group (group III) which received saline 5 days before and 21 days after a single cisplatin injection of the same dose. Cisplatin and L-carnitine group (group IV) and cisplatin and L-carnitine recovery group (group V) corresponded to groups II and III respectively in their time design, but L-carnitine was used instead of saline. Liver specimens were subjected to hematoxylin and eosin, Masson trichrome, silver staining as well as immunohistochemical Caspase-3 examination and measurements utilizing image analysis technique ...
Purpose: Enteric glial cells (EGC) exert a critical role in the structural integrity, defense and metabolic function of enteric neurons. Diabetes mellitus (DM) is a chronic disease characterized by metabolic disorders and chronic autonomic neuropathy. Quercetin supplementation, which is a potent antioxidant, has been used in order to reduce the effects of diabetes-induced oxidative stress. The purpose of this research was to investigate the effects of quercetin supplementation in the drinking water at a daily dose of 40 mg on the glial cells and neurons in the jejunum of diabetic rats.Material and Methods: Twenty 90-day-old male adult Wistar rats were split into four groups: normoglycemic control (C), normoglycemic control supplemented with quercetin (Q), diabetic (D) and diabetic supplemented with quercetin (DQ). After 120 days, the jejunums were collected and immunohistochemical technique was performed to label S-100-immunoreactive glial cells and HuC/D-immunoreactive neurons.Results: An intense
The objective of the present study was to examine the effects of prenatal exposure to ethanol on motor performance, emotionality, learning and memory in young-adult, male Wistar rats. Alcohol was delivered to the pregnant dams intragastrically, throughout gestation days (GD) 7-20, at the dose of 6 g/kg/day resulting in the peak blood alcohol concentration (BAC) of 350 mg/dl as assessed on GD 20. Isocaloric intubation and untreated control groups were included. Alcohol exposed rats were not impaired in the rotarod/accelerod tests. Their behavior in the open field and plus maze suggested increased neophobia. Hyperactivity was not observed. In the spatial-navigation task in the water maze, by the middle of the training, fetal alcohol rats showed a tendency towards a slower place acquisition compared to controls, but statistical analysis of the data did not yield between-group differences significant. Towards the end of the training, all rats reached a similar performance level. No detectable ...
Rationale and Objectives 5-HT6 receptors are mainly expressed in brain areas associated with learning and memory. Several studies have reported procognitive effects of both 5-HT6 agonist and antagonists. However, the exact mechanism5 HT6 receptor modulation has not been properly studied especially in the context of cholinergic functions, cerebral blood flow (CBF), brainderived neural factor (BDNF), oxidative stress, and behavioral changes. Methods In the present study, memory impairment was induced in albino Wistar rats by two doses of intracerebroventricular (ICV) injection of treptozotocin (STZ, 3 mg/kg) on first and third day. These rats were evaluated in a battery of behavioral tasks after 14 days from the first day of ICV-STZ.Results Significant memory impairment was seen when ICV-STZ induced rats are assessed by Morris water maze, novel object recognition, social recognition, and passive avoidance tests. There was a significant reduction in CBF, increased oxidative stress (MDA, GSH, and ...
INTRODUCTION Central cardiovascular regulation in haemorrhagic shock is influenced by many neuronal systems, including the melanocortinergic and histaminergic systems. The aim of the study was to examine the involvement of the his-taminergic system in the resuscitating effect of melanocortin type 4 (MC4) receptor agonist Ro 27-3225. MATERIALS AND METHODS Studies were carried out on male Wistar rats subjected to irreversible haemorrhagic shock with a mean arterial pressure (MAP) of 20-25 mmHg. In the 5th minute of critical hypotension, the animals were injected intracere-broventricularly (icv) with Ro 27-3225 or a 0.9% NaCl solution (control group). RESULTS Hypovolaemia was accompanied by a decrease in pulse pressure (PP), heart rate (HR) and renal blood flow (RBF). In the control group, there were no increases in the measured parameters, and a survival rate of 2 h was 0%. Ro 27-3225 (10 nmol, icv) evoked long-lasting rises in MAP, PP and RBF as well as 100% survival at 2 h. Pre-treatment with ...
Nonsynaptic mechanism changes, particularly the enhancement of NKCC1 expression in the dentate gyrus (DG) after 4 weeks of ethanol consumption, motivate the present work, in which rats were submitted to a period of chronic consumption (12 weeks). Four groups of six animals (6-week-old male Wistar rats) were formed, including the control (C), ethanol 1 (El), ethanol 2 (E2) and ethanol 3 (E3) groups. The rats in the El, E2 and E3 groups were treated daily with a 30% v/v solution of ethanol, administered via oral gavage (1.0, 2.0 and 3.0 g/kg, respectively). Nonsynaptic epileptiform activities (NEA) were induced by means of the zero-Ca2+ and high-K+ model using hippocampal slices and were recorded in the DG. The presence of NKCC1, KCC2, a1Na(+)/K+-ATPase and GFAP immunoreactivity was analyzed. The results demonstrate that alcohol consumption changes NEA, and these changes are more prominent at the lower dosage. An increase in the DC shifts associated with epileptiform discharges was present with ...
UNLABELLED Angiotensin II has articularly shown to play a key role in the regulation of inflammatory processes in hypertension. AIM The present study aims to correlate the angiotensin II-induced hypertension 4th systemic inflammation. MATERIAL AND METHODS We conducted an experimental study on Wistar male rats who received Ang II via subcutaneous miniosmotic pumps for 2 weeks. Rats were exposed to a 12h light /12h dark cycle. Sham rats were used as control. Systolic load pressure measurements and a flow cytometric analysis of lymphocyte surface markers were performed. After 14 days, the animals were euthanized under anesthesia with ylazine/ketaniine. RESULTS Systolic BP progressively and significantly increased in rats 4th Ang II chronic infusion. We observed a statistical significant difference (p = 0.00001), in terms of T lymphocytes percentage between control rats plasma and Ang H treated rats lasma, in 14 days. CONCLUSIONS Angiotensin II is an important mediator of hypertension and directly
Electrophysiology. Experiments were performed on posterior hypothalamic slices from 3- to 4-week-old male Wistar rats. After decapitation, the brain was placed in an ice-cold solution made up of (in mm): 208 sucrose, 26 NaHCO3, 10 glucose, 2.1 MgCl2, 1.8 KCl, 1.5 CaCl2, and 1.2 KH2PO4. The solution was bubbled to a pH of 7.4 with a mixture of 95% O2 and 5% CO2. Slices were cut at a thickness of 300-400 μm as described previously (Stevens and Haas, 1996) and transferred to artificial CSF (ACSF), which had the same composition as above except that the sucrose was replaced with 125 mm NaCl. The slices were held in ACSF at room temperature (22-24°C) for at least 1 hr before starting the recording. The slices were then transferred to a thermostat-controlled recording chamber, in which they were submerged in ACSF with a flow rate of 1.8-2.0 ml/min−1 and a temperature of 32-33°C. Intracellular recordings were obtained using sharp glass microelectrodes filled with K+acetate (4 m) with resistances ...
Domestic rats differ from wild rats in many ways: they are calmer and significantly less likely to bite, they can tolerate greater crowding, they breed earlier and produce more offspring, and their brains, livers, kidneys, adrenal glands, and hearts are smaller. Scientists have bred many strains or lines of rats specifically for experimentation. Most are derived from the albino Wistar rat, which is still widely used. Other common strains are the Sprague Dawley, Fischer 344,[8] Holtzman albino strains, Long-Evans, and Lister black hooded rats. Inbred strains are also available but are not as commonly used as inbred mice. Much of the genome of Rattus norvegicus has been sequenced.[9] In October 2003, researchers succeeded in cloning two laboratory rats by nuclear transfer. This was the first in a series of development that have begun to make rats tractable as genetic research subjects, although they still lag behind mice, which lend themselves better to the embryonic stem cell techniques ...
Fourteen 12- to 16-week-old male Wistar rats weighing 200 to 400 g (Scanbur AB, Sollentuna, Sweden) were used. All animals were treated according to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. With approval from the Linköping regional animal ethics review board, the rats were anesthetized, and a corneal stromal suture was placed 1.5 mm from the temporal limbus as previously described. 13 A second stromal suture was placed at the 3 oclock position, 1.5 mm from the nasal limbus, to provide additional tissue samples for ex vivo analysis. Left eyes served as negative controls. At 0, 6, and 12 hours and 1, 2, 3, 4, 5, and 7 days after the initial surgery, the rats were anesthetized with intraperitoneal injection of dexmedetomidine (Orion Pharma AB, Sollentuna, Sweden) and xylazine (Pfizer AB, Sollentuna, Sweden), and laser scanning in vivo confocal microscopy (IVCM) of the corneas was performed after topical administration of 1% tetracaine. The number of rats ...
Download Free Full-Text of an article EFFECT OF TAMOXIFEN ON LAYER UTERUS AND THE NUMBER OF OFFSPRINGS IN FEMALE WISTAR RATS in Confrence
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Wound healing activity of Cynodon dactylon (L.) Pers. in albino wistar rats By Anand Kumar*1 and Pranita Kashyap2 1Head, Department of Pharmacy, ...
Wound healing activity of Cynodon dactylon (L.) Pers. in albino wistar rats By Anand Kumar*1 and Pranita Kashyap2 1Head, Department of Pharmacy, ...
PURPOSE: To present an animal model to assess the effects of end-to-side innervation in the heterotopically transplanted model with reduced chances of neural contamination.METHODS:The medial portion of the gastrocnemius muscle in wistar male rats was isolated and its pedicle dissected and performed a flap in the abdominal portion. To prevent neural contamination in the abdominal region, the muscle was wrapped with a Goretex(r) sheet. The specimens were divided into 2 groups (G). In G1 was performed an end-to-end suture between tibial nerve of the gastrocnemius and femoral motor nerve and between the saphenous sensory nerve and the motor nerve. In G2 was performed a end-to-side suture between the tibial nerve and the motor femoral and between the tibial nerve and saphenous motor nerve. The specimens were evaluated 60 days later to check the structure of the neurorraphy. Sections were obtained proximal and distal to the coaptation site.RESULTS:The medial gastrocnemius muscle had the advantage of ...
Many methods are used to evaluate ischemia reperfusion injury, but the most employed one is the histological study. However, it only demonstrates on mucosal tunic, the index of lesion and morphologic preserved cells, but not the index of viable functional cells, present in the sample. With this purpose, a colorimetric method was used, employing Methyl Thiazolyl Blue (MTT). The experiment was conducted in 30 Wistar male rats, distributed in 3 groups: Group Control (GC), Group ischemia and reperfusion-1 (GIR-1) and Group ischemia and reperfusion-3 (GIR-3), with 10 animals each. The Groups GIR-1 and GIR-3 were submitted to intestinal ischemia for 30 minutes by a false ligature of superior mesenteric artery, and submitted to euthanasia after 1 and 3 days of reperfusion, when material was picked for absorbency and histological procedures. It was observed a smaller proportion of viable cells and a larger degree of mucosal lesion in GIR-3 group (p,0.05), while GC group was the one with the larger ...
The present study investigated the sensitivity to stress and diazepam in weaning (21-day old) Wistar rats. A single 15-min session of forced swimming was used to induce anxiety-like behavior. The group that was forced to swim exhibited...
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Obsevation: Endotoxic shock is a life-threatening condition and its sequelae represent an array of clinical symptoms that encompass systemic inflammation, coagulopathy, and abnormalities of the renal, hepatic, pulmonary and hematologic systems, followed by multi-organ failure. Furthermore, systemic failure leads to an unfavorable environment in the gut that causes an imbalance in the homeostasis of intestinal microbiota. In the present study, the effect of symbiotics as adjuvant therapy in endotoxic shock was investigated. Adult male Wistar rats were randomized into three groups: Control group received a single intraperitoneal injection of physiological saline solution, and the other two groups received either a symbiotic formulation or a placebo daily, for one week, followed by the administration of a single lethal dose of lipopolysaccharide (LPS) from E. coli (20 mg/Kg, IP). Results: The symbiotic formulation used improved the survival rate of LPS-treated rats, ameliorated the clinical symptomatology,
Obsevation: Endotoxic shock is a life-threatening condition and its sequelae represent an array of clinical symptoms that encompass systemic inflammation, coagulopathy, and abnormalities of the renal, hepatic, pulmonary and hematologic systems, followed by multi-organ failure. Furthermore, systemic failure leads to an unfavorable environment in the gut that causes an imbalance in the homeostasis of intestinal microbiota. In the present study, the effect of symbiotics as adjuvant therapy in endotoxic shock was investigated. Adult male Wistar rats were randomized into three groups: Control group received a single intraperitoneal injection of physiological saline solution, and the other two groups received either a symbiotic formulation or a placebo daily, for one week, followed by the administration of a single lethal dose of lipopolysaccharide (LPS) from E. coli (20 mg/Kg, IP). Results: The symbiotic formulation used improved the survival rate of LPS-treated rats, ameliorated the clinical symptomatology,
In situ hybridisation showing localisation of Mrap, Mrap2 and Mc2r in foetal and adult Wistar rat adrenal glands. (A-G) Foetal Wistar rat adrenal glands (embr
Bumetanide is a selective inhibitor of the Na(+)-K(+)-Cl(-)-co-transporter 1(NKCC1). We studied whether bumetanide could affect axonal growth and behavioral outcome in stroke rats. Adult male Wistar rats were randomly assigned to four groups: sham-operated rats treated with vehicle or bumetanide, and ischemic rats treated with vehicle or bumetanide. Endothelin-1 was used to induce focal cerebral ischemia. Bumetanide administration (i.c.v.) started on postoperative day 7 and continued for 3 weeks. Biotinylated dextran amine (BDA) was injected into the right imotor cortex on postoperative day 14 to trace corticospinal tract (CST) fibers sprouting into the denervated cervical spinal cord ...
Methods. Animals and anesthetics. Animal handling and experimental procedures were reviewed and approved by the ethical committee for animal care and use of the Royal Netherlands Academy for Sciences, acting in accordance with the European Community Council directive of 24 November 1986 (86/609/EEC) and the ARVO statement for the use of animals in Ophthalmic and Vision Research. The procedure to induce transient ischemia followed by reperfusion has been described in detail previously [25-27]. In short, adult male Wistar rats (Harlan, the Netherlands) weighing 200-300 g were anesthetized and mounted onto a stereotactic frame (Stoelting, Wood Dale, IL). A steel 30-gauge infusion needle (BD Biosciences, Alphen aan den Rijn, The Netherlands) connected to a saline reservoir was placed in the middle of the anterior chamber of the left eye. The reservoir was opened and lifted to 1.70 m. Ischemia was confirmed by the white anemic fundus reflex. After 60 min of ischemia, the reservoir was lowered and ...
Affiliation:鶴見大学,歯学部,准教授, Research Field:矯正・小児・社会系歯学,Functional basic dentistry, Keywords:酒石酸耐性酸性ホスファターゼ,adult Wistar rats,骨芽細胞,tension side,osteoblast,tooth movement,破骨細胞,serum BGP,歯の移動,血清BGP, # of Research Projects:5, # of Research Products:0
Myocardial hyperfunction is experimentally modeled by coarctation of the aorta in mature (6-8-month-old) and senescent (26-28-month-old) Wistar rats. During the emergency phase of cardiac...
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An increased incidence of myocardial necrosis or fibrosis was observed in long-term (6-12 months) studies in rats and mice (exposure 8 times human AUC exposure in rats and somewhat equivalent to human Cmax exposure in mice). Findings were not seen at lower doses. In dogs no cardiotoxicity was observed following 12 months of oral dosing at doses that resulted in maximum plasma concentrations (Cmax) 14 times the Cmax exposure in humans receiving a 12 mg/day therapeutic dose or in Wistar rats at Cmax exposures 15 times human Cmax exposure. There is no evidence that similar lesions occur in humans. ...