Although it is now well documented (6, 8) that pial as well as intracerebral vessels are amply supplied with sympathetic adrenergic nerves which, as shown for pial arteries, fulfill ultra-structural...
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Differences in spontaneous allograft acceptance after liver transplantation among inbred rat strains might be explained by variation in the local production of TNF-α as a potent mediator of the inflammatory response. In this study, we hypothesize that nucleotide differences in the rat Tnf gene influence TNF-α protein expression. As such, polymorphisms in the Tnf gene may also provide a possible explanation for differences in survival of allogeneic liver grafts among inbred rat strains. We therefore investigated the capacity of mononuclear cells to produce TNF-α in response to a mitogenic stimulus and the Tnf locus was sequenced in six different inbred rat strains. Among the six strains (AUG, BN, DA, LEW, PVG and WF), 44 nucleotide differences including 36 single nucleotide polymorphisms (SNPs), five simple sequence length polymorphisms, two deletions and one insertion, were found in the Tnf gene. Although, the inbred rat strains differed significantly in mean levels of maximum TNF-α ...
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Alterations in protein synthesis in primary cultured rat liver parenchymal cells were examined after their exposure to the potent carcinogens, polychlorinated biphenyl (PCB) congeners. Co-planar PCB congeners (3,4,5,3′,4′-PCB and 3,4,5,3′,4′,5′-PCB) (10 nM) induced a protein, the Mr of which was 25,000 (25 k protein) under denaturing conditions. However, non-co-planar PCB congeners and several xenobiotics, which induce microsomal proteins, did not induce the 25 k protein. By using immunoblotting, the 25 k protein was identified as glutathione S-transferase P-form (GST-P, 7-7, EC 2.5.1.18).. ...
The expression and induction of the cytochrome P450 2B1/2 isoenzyme is heterogeneous, exhibiting a regional pattern in the intact liver and a varied response to phenobarbital in isolated cultured hepatocytes. We report that P450 2B1/2 immunostaining of hepatocytes isolated from the perivenous liver region and cultured in the presence of phenobarbital is much stronger than that of cells identically treated but isolated from the periportal region. P450 2B1 mRNA, quantified by a sensitive and specific RNAase protection assay, is also preferentially induced in perivenous hepatocytes, demonstrating that the difference in induced expression is at the pretranslational level. Our results suggest that perivenous and periportal hepatocytes are differentially imprinted to retain regiospecific factors governing their inducibility after isolation.. ...
Visual pathways and acuity hooded rats.: Three experiments on the effects of lesions of the visual system on contrast-detection in hooded rats are described, in
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Top: Relative distribution plots of GA, GB, GC, GJ, and BB obtained from buckets, i.e., automatically integrated spectral segments of 0.02 ppm, of the H-12 sin
IRVINE, Calif., Nov. 9, 2015-- Sabra Health Care REIT, Inc. today announced results of operations for the third quarter of 2015.. For the third quarter of 2015, Normalized FFO, Normalized AFFO and net income attributable to common stockholders per diluted common share were $0.58, $0.53 and $0.24, respectively, compared to $0.51, $0.51 and $0.31, respectively, for the...
Статья "Contents and abstracts" в журнале "Философия и общество", Выпуск №4(68)/2012
Primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 X 10(6) isolated hepatocytes at 37 degrees C in Dulbeccos medium supplemented wi
We have studied the effect of glucagon on the expression of a triiodothyronine (T3) and carbohydrate-inducible mRNA sequence (mRNA-S14) in rat liver that undergoes a threefold diurnal variation (peak, 2200 h; nadir, 0800 h). Glucagon injection into euthyroid rats (25 micrograms/100 g body wt i.p., three doses at 15-min intervals) during the nocturnal plateau of mRNA-S14 caused a monoexponential disappearance of this sequence (t1/2, 90 min) accompanied by a 90% reduction in the transcriptional rate in a nuclear run-off assay, indicative of a near total reduction of synthesis. This effect was markedly attenuated in rats treated with T3 (200 micrograms/100 g body wt i.p.) 24 h before glucagon injection. When T3 was given 15 min after glucagon, the glucagon-initiated decline in mRNA-S14 was reversed within 90 min, suggesting a rapid interaction between the two hormones in the evening. Curiously, administration of T3 alone at this hour did not affect a significant increase in mRNA-S14. At 0800 h, ...
Reference: Klimenko A.I., Age-related characteristics of the concentration of RNA, DNA and proteins in the nuclei of rat liver cells following hydrocortisone induction, Voprosy meditsinskoi khimii, 1971, vol: 17(6), 615-619 ...
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Fakulteta za informacijske študije v Novem mestu je pred kratkim v Dolenjskih Toplicah organizirala zaključni dogodek projekta COSMOS. V interdisciplinarnem projektu s področja matematičnega modeliranja zapletenih sistemov je sodelovalo 15 študentov, ki so pod vodstvom 15 mentorjev pripravljali doktorat.
This study was conducted to compare the effects of n-6 linoleic acid and n-3 linolenic acid on lipid peroxidation and the activities of enzymes defending against oxidation, which are involved in the tumor promotion, and histolOgical changes of hepatocarcinogen treated rat liver. In this study, weanling male Sprague-Dawley rats were fed one of three diets, containing 15% (w/w) of beef fat (BF), com oil (CO) or perilla oil (PO), for 11 weeks. During the 3rd week, experimental groups were injected with 2-AAF (50 mg/kg of BW) intraperitoneally 3 times. Findings show that the com oil diet group has greater liver MDA content than the beef fat and perilla oil diet groups. Also, it is observed that the perilla oil diet group has lower MDA content than beef fat and com oil diet groups, even though perilla oil is more desaturated than beef fat and com oil. In terms of activity, mixed-function oxidase activity is not Significantly affected by the different dietary fats and 2-AAF treatment. GSH-peroxidase, ...
The acute pulmonary responses after exposure to sulfur and nitrogen mustards are well documented whereas the late pulmonary effects are not. With a novel focus on the immune system this paper investigate whether late phase pulmonary effects developed in rats exposed to the nitrogen mustard melphalan are linked to the acute responses and whether the reactions are genetically regulated. The DA rat strain was used to establish a lung exposure model. Five other inbred rat strains (PVG, PVG.1AV1, LEW, WF and F344) were compared within the model at selected time points. All rat strains displayed a biphasic pattern of leukocyte infiltration in the lungs, dominated by neutrophils 2 days after exposure and a second peak dominated by macrophages 29 days after exposure. The number of macrophages was higher in the DA rat compared with the other strains. The infiltration of lymphocytes in the lungs varied in both time of appearance and magnitude between strains. The quantity of collagen deposition in the ...
Sabra Dipping Company, LLC (Sabra), maker of Americas favorite hummus, broke ground this morning on an expansion of the worlds largest hummus-making facility, based in Chesterfield County, VA. The 40,000 square foot expansion will enable Sabra to further expedite product delivery to retail, which means getting freshly-made hummus into consumer hands and homes sooner. The new addition more than doubles Sabras finished goods storage capacity, lays the groundwork for future packaging customization capabilities and will result in an improved carbon footprint. Sabra expects to add . . .
Evidence was presented to indicate that the binding of benzo(a)pyrene (50328) (BaP) to a protein in rat liver cytosol facilitates its oxidation by microsomal enzymes. Cytosols were prepared from male Sprague-Dawley-rats. Sephadex G-100 gel permeation chromatography of rat liver cytosol saturated with carbon- 14 labeled BaP resulted in two peaks of protein bound radioactivity. Glutathione-S-transfe
Sedlis, A and Stone, D F., Experimental carcinogenesis in pregnant mice, a preliminary report. (1965). Subject Strain Bibliography 1965. 820 ...
Chlorophyll (Ch) a, b and total content (µg g FW−1) measured in fully developed leaves of Citrus macrophylla seedlings grown for 25 days in B-normal (50 μM,
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Tissue preparation. Demonstration of basic histology techniques including fixation, embedding, sectioning, staining and observation of a glass microslide operation. Orig.
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Periodical: Sokoloff, Louis, Martin Reivich, Charles Kennedy, M. H. Des Rosiers, C. S. Patlak, K. D. Pettigrew, O. Sakurada, and M. Shinohara. The [14C]Deoxyglucose Method for the Measurement of Local Cerebral Glucose Utilization: Theory, Procedure, and Normal Values in the Conscious and Anesthetized Albino Rat. Journal of Neurochemistry 28, 5 (1977): 897-916. Article. 20 Images ...
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The arthritis-susceptible DA rat is one of the most commonly used rat strains for genetic linkage analysis and is instrumental for the identification of many genetic loci. Even though DA rats were kept as inbred lines at different institutes and suppliers, it became obvious that the various breeding
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Figure 3: IL-6 serum levels of TIMP-3-treated rats compared to saline-treated rats exposed to liver I/R injury. Serum samples were quantified for IL-6 by ELISA as per manufacturer s protocol. IL-6 levels on the Y-axis (pg/ml) are shown for TIMP-3-treated rats and saline-treated rats prior to ischemia/ reperfusion injury. The X-axis indicates the length of reperfusion time for TIMP-3-treated and saline-treated ...
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ARFC appear in the spleen after transplantation of organs or tissues and immunization with nucleated cells between isohistogenic rat strains. The allorosette formation of rat spleen cells and T...
How is micrograms per liter abbreviated? µg/L stands for micrograms per liter. µg/L is defined as micrograms per liter very frequently.
TY - JOUR. T1 - Enhancement by neuropeptide γ of DNA synthesis stimulated with EGF or TGFα in primary cultures of adult rat hepatocytes. AU - Hasegawa, K.. AU - Carr, B. I.. PY - 1993. Y1 - 1993. N2 - DNA synthesis was stimulated by primary (complete) mitogens such as epidermal growth factor (EGF) or transforming growth factor α (TGFα) in adult rat hepatocytes in primary culture. Addition of neuropeptide γ (10-9 M) to the hepatocyte culture amplified the stimulatory effect of EGF or TGFα by 5- to 6-fold. Neuropeptide γ-amplified DNA synthesis was observed in a dose-dependent manner with maximal effect at 10-9 to 10-7 M, although it effect was significantly observed at as low as 10-10 M. Neuropeptide γ by itself did not stimulate DNA synthesis in the absence of EGF. Amplification of DNA synthesis by neuropeptide γ was observed when hepatocytes were cultured in Williams medium E, but not in Leibovitz L-15 medium, suggesting that culture medium enriched with nutrients is required for ...
TY - JOUR. T1 - Receptor-mediated uptake of human α1-acid glycoprotein into liver parenchymal cells in mice. AU - Matsumoto, Kazuaki. AU - Nishi, Koji. AU - Kikuchi, Mari. AU - Watanabe, Hiroshi. AU - Nakajou, Keisuke. AU - Komori, Hisakazu. AU - Kadowaki, Daisuke. AU - Suenaga, Ayaka. AU - Maruyama, Toru. AU - Otagiri, Masaki. N1 - Funding Information: Acknowledgments: This research was supported Grants-in-aid in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) (18590035 and 20390161).. PY - 2010. Y1 - 2010. N2 - Human α1-acid glycoprotein (AGP), a serum glycoprotein, is thought to have anti-inflammatory effects by a mechanism that is not well understood. In this study, we investigated the pharmacokinetics of AGP in mice using enzymatically modified AGP (AGP with the sialic acids removed, asialo-AGP, and with both sialic acids and galactose removed, agalacto-AGP). It was observed that AGP was eliminated from the circulation slowly, and ...
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DRH strain rats are a unique experimental model in which to study chemically induced liver carcinogenesis. They have been selected from a closed colony of Donryu rats fed 3′-Me-DAB by sister-brother mating for over 40 generations. Their resistance to hepatocarcinogens has been suggested to be genetically dominant (15) . In the present study, we focused on genetic control in the induction of preneoplastic lesions. Five phenotypic parameters were selected to quantitatively describe the preneoplastic lesions; i.e., the number of GST-P-positive foci, the percentage of liver area occupied by EAF, the size of the foci, and the amounts of GST-P as well as GGT mRNA. Their phenotypic values were mutually closely interrelated [for instance, percentage of liver area occupied by EAF and GST-P mRNA (r = 0.763)], but still they seemed under differential genetic control by combinations of host loci. No single phenotypic parameter was controlled by a single locus. The contributions of the loci to phenotype ...
Oral The test material was investigated in a key study designed to be compatible with OECD Guideline for Testing of Chemicals No 420 Acute Oral Toxicity - Fixed Dose Method (2001) and Method B.1 bis Acute Toxicity (Oral) of Commission Regulation (EC) No 440/2008. Following a sighting test at a dose level of 2000 mg/kg, an additional four fasted female animals were given a single oral dose of the test item, as a solution in arachis oil BP, at a dose level of 2000 mg/kg body weight. Clinical signs and body weight development were monitored during the study. All animals were subjected to gross necropsy. There were no deaths, no signs of systemic toxicity, all animals showed expected gains in body weight and no abnormalities were noted at necropsy. The acute oral median lethal dose (LD50) of the test item in the female Wistar strain rat was considered to be greater than 2000 mg/kg body weight. Inhalation According to REACH Annex VIII, Section 8.5, Column 2, information on acute toxicity shall be ...
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Changes in binding of dexamethasone (9α-fluoro-11β, 17α,21-trihydroxy-16α-methylpregna-1,4-diene-3,20-dione) to its receptors in regenerating rat liver after 70% hepatectomy were examined. Specific receptors for dexamethasone in the liver remnants of adrenalectomized rats decreased significantly during the period of DNA synthesis after 70% hepatectomy; then, they increased to above the control values between Days 4 and 7 after partial hepatectomy and subsequently returned to the control values. During the period of DNA synthesis, decreased binding was observed in partially hepatectomized rats with or without adrenals, but later enhanced binding was not prominent in rats with adrenals.. ...
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Anti-CD90 / Thy1 antibody [MRC OX-7] (ab225) has been cited in 35 publications. References for Mouse, Rat, Rabbit in Flow Cyt, ICC/IF, IF, IHC-FrFl, IHC-P, WB
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