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According to recent investigations neopterin (a pyrazinopyrimidine derivative) is a biochemical marker that reflects the activity of the proinflammatory immunocellular system of the synovial tissue in rheumatoid arthritis (RA). Interferon gamma, derived from antigen activated T lymphocytes, stimulates macrophages to synthesise and release neopterin into the culture supernatant in vitro. To extend this in vitro model to a clinical level a sensitive new radioimmunoassay technique was used to measure neopterin concentrations in the synovial fluid (SF) of 17 patients with active RA, nine with osteoarthritis, and six with acute gout, and in that of 12 controls undergoing meniscectomy. The SF neopterin concentrations were significantly higher in patients with RA than in the other groups of patients, particularly the controls. Multivariant analysis showed that SF neopterin concentrations correspond better with the systemic inflammatory activity of RA than with the local disease activity of the knee ...

In this paper a method for the radioimmunological determination of triamcinolone acetonide (TAAc) in plasma is described. The antiserum used in the radioimmunological determinations showed high...

Oestrus was synchronised with PGF2-alpha in four pluriparous West African Dwarf (WAD) goats and blood collected through jugular venupuncture. The length of the oestrous cycle and oestrus duration were observed in three successive cycles while peripheral plasma progesterone and oestradiol-17B levels were determined by the radioimmunoassay technique. The mean oestrous cycle lengths and oestrus duration were 19.4 ± 1.7 d and 33.3 ± 6.6 h, respectively. Progesterone levels were very low during the first four days of the cycle (day 1 = day of oestrous) with the lowest level of 0.3 ± 0.02 ng/ml being recorded on day 2 of the cycle. There was a steady rise in the levels during the cycle until day 15 when the highest level of 2.2 ± 0.05 ng/ml was recorded which was followed by a sharp decline on day 20 (P ,0.05). The profile of oestradiol-17B was erratic during the cycle. Mean maximum and minimum concentrations were recorded on day 1 (152.6 ± 31.6 ng/ml) and day 2 (58.8 ± 22.6 ng/ml), ...

Huge collection of Rosalyn S. Yalow quotes. Born on 1921-07-19 Died on 2011-05-30. Nickname: Rosalyn Sussman. Radioimmunoassay, (RIA) Rosalyn Sussman Yalow was an American medical physicist, and a co-winner of the 1977 Nobel Prize in Physiology or Medicine for development of the radioimmunoassay technique. She was the second American woman to be awarded the Nobel Prize in Physiology or Medicine after Gerty Cori.

Abstract: Heparinized blood samples were taken from male immature and mature camels of the Shaalah breed, housed at the University Animal farm, during the rutting and non-rutting period. Other blood samples were also collected from camels slaughtered at defined seasons (summer, autumn, winter and spring) and from the Buraydah slaughter-house. In addition, specimens from the testes were also taken to confirm the difference between the immature and the mature animals during the non-rutting and rutting seasons. The plasma obtained from the collected blood samples was used for estimation of the following hormones, Melatonin (MLT), Follicle Stimulating Hormone (FSH), Leutinizing Hormone (LH), Testosterone and Prolactin (PRL) using the radioimmunoassay technique. Specimens of testes tissue were fixed in calcium formol, processed for histological examination using standard procedures and stained with H&E. The results clearly differentiated the samples as immature and mature during the non-rutting and ...

TY - JOUR. T1 - Bleomycin serum pharmacokinetics as determined by a radioimmunoassay and a microbiologic assay in a patient with compromised renal function. AU - Crooke, Stanley T.. AU - Luft, Friedrich. AU - Broughton, Alan. AU - Strong, James. AU - Casson, Kay. AU - Einhorn, Lawrence. PY - 1977/4. Y1 - 1977/4. N2 - Serum and plasma bleomycin concentrations were determined in a patient with renal dysfunction at two creatinine clearances. The results obtained with a new radioimmunoassay and the microbiologic assay were compared. It was shown: 1) that the clearance of bleomycin from the blood is markedly retarded in severe renal dysfunction, 2) that clearance of bleomycin varies with creatinine clearance, 3) that bleomycin is probably not dialyzable, 4) that determinations on serum and plasma were equivalent, and 5) that the radioimmunoassay and microbiologic assays gave equivalent results (P , 0.001).. AB - Serum and plasma bleomycin concentrations were determined in a patient with renal ...

TY - JOUR. T1 - Endothelium-independent contractions to jvg-monomethyl-l-arginine in canine basilar artery. AU - Katušić, Zvonimir S.. PY - 1991/11. Y1 - 1991/11. N2 - Background and Purpose: NG-substituted analogues of L-arginine are potent and selective inhibitors of nitric oxide synthase(s). The present study was designed to determine the effects of these analogues on the vascular smooth muscle of isolated canine basilar arteries. Methods: Basilar artery rings without endothelium were suspended for isometric tension recording in Krebs-Ringer bicarbonate solution bubbled with 94% O2-6% CO2 (temperature=37°C, pH=7.4). A radioimmunoassay technique was used to determine the levels of guanosine 3,5-cyclic monophosphate (cyclic GMP). Results: NG-Monomethyl-L-arginine (L-NMMA) caused concentration-dependent contractions, whereas the D-enantiomer and NG-nitro-L-arginine did not. Contractions to L-NMMA were reduced in the presence of L-arginine but not in the presence of D-arginine. Superoxide ...

to achieve the student with the theoretical skills that allow an adequate knowledge of physical and biological phenomena at the base of radioimmunoassay techniques.. ...

Figure 2: The Effects of Endogenous Thyroglobulin Antibody on the Measurement of Serum Thyroglobulin using Immunoenzymatic (Sandwich) Method. Thyroglobulin Immunoassays. Several immunoassays are commercially available to measure thyroglobulin including the competitive radioimmunoassay (RIA) and the one-step immunoenzymatic (sandwich) assay (IMA). The RIA method is older and more labor intensive compared to IMA. In the RIA method, thyroglobulin competes with 125I labeled human thyroglobulin for a rabbit antibody against human thyroglobulin. After six days of incubation, thyroglobulin - antibody complexes are precipitated with a second anti-rabbit antibody. This method can detect both free thyroglobulin and thyroglobulin bound to antibody. The radioactivity is inversely proportional to the thyroglobulin concentration (2). In contrast to RIA, the IMA can be performed within one hour. The thyroglobulin in the patients sample is mixed with a biotinylated reagent that contains four monoclonal ...

We describe a radioimmunoassay procedure for human somatotropin using second antibody - polyethylene glycol (PEG) combination for the separation of antibody bound and free antigen. The assay is done...

If indirect immunofluorescence assay (IFA) (ANN1C, ANN2C, ANN3C, PCA1C, PCA2C, PCTRC, AMPHC, CRMC, AGN1C) is indeterminate, then paraneoplastic autoantibody Western blot is performed at an additional charge.. If IFA pattern suggest NMO/AQP4-IgG, then NMO/AQP4-IgG FACS is performed at an additional charge.. If NMO/AQP4-IgG FACS screen assay requires further evaluation, then NMO/AQP4-IgG FACS titration assay is performed at an additional charge.. If IFA patterns suggest CRMP-5-IgG, then CRMP-5-IgG Western blot is performed at an additional charge.. If IFA patterns suggest GAD65 antibody, then GAD65 antibody radioimmunoassay is performed at an additional charge.. If IFA patterns suggest neuronal voltage-gated potassium channel-complex autoantibody, then VGKC-complex antibody IPA is performed at an additional charge.. If VGKCC ,0.00, LGI1-IgG CBA, CSF and CASPR2-IgG CBA, CSF are performed at an additional charge.. If IFA patterns suggest amphiphysin antibody, then amphiphysin Western blot is ...

490 blood samples have been examined for the comparison of the various methods concerned with the detection of the Australia Antigen (Au). The following methods have been comparatively examined: immunodiffusion (ID) counterelectrophoresis (CEP), complement fixation (CF) passive hemoagglutination (HA) and solid phase radioimmunoassay RIA. On the basis of the results so far obtained the RIA appears the most sensitive and specific method, followed by the HA. In order to obtain reproducible results for diagnostic purposes we suggest to carry out routinely two different techniques together (possibly RIA and HA), while for screening purposes just one technique, choosed between the two most indicative ones, shall be employed.

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An immobilized immunoadsorbent for use in immunoassay and radioimmunoassay procedures includes, in a preferred form, a mass of superficially porous refractory particles having a water insoluble polymer, such as dextran, bonded thereto. The polymer in turn has bonded to it, by covalent bonds, antibodies capable of binding a specific antigen thereto and stoichiometrically releasing the bound antigen by the use of a rinsing solution which does not adversely affect the binding of the antibody to the polymer. Preferably, each of the porous particles includes an impervious core which has joined thereto sufficient layers of microparticles to form an outer porous coating on the core. Methods of forming such an immunoadsorbent and for using the same and structure for supporting the same are also disclosed.

The in vivo release of cholecystokinin (CCK)-like material (CCKLM) was measured in the frontal cortex of freely moving rats using the microdialysis technique combined with a sensitive radioimmunoassay. Local perfusion of K+ (100 mM)-enriched artificial CSF resulted in a 10-fold increase in CCKLM outflow, as compared with that occurring under basal resting (K+ = 3.0 mM) conditions, and this effect could be completely prevented by removal of Ca2+ in the perfusing fluid. Chromatographic analyses demonstrated that CCK-8S contributed to 70% of CCKLM. Stressful stimuli such as a 2-min exposure to diethyl ether and a 30-min restraint produced a marked but transient increase in cortical CCKLM release. In addition, anxiety-like behavior induced by the systemic administration of yohimbine (5 mg/kg i.p.) was associated with a long-lasting enhancement in the peptide outflow. Pretreatment with the potent anxiolytic drug diazepam (5 mg/kg i.p., 5 min before each condition), which exerted no effect on its own,

Anti-warfarin antiserum was prepared in rabbits by immunization with a synthesized warfarin antigen, 4-azo-warfarin human serum albumin, which possesses two enantiomorphic haptenic sites of warfarin on the molecule. The antiserum recognized both R- and S-warfarin to the same degree, 50% cross reactivities of racemic warfarin, respectively. One of the warfarin metabolites, racemic warfarin alcohol, showed 1% cross reactivity, and R- or S-warfarin alcohol have half the reactivity of the racemic alcohol. Rabbit plasma warfarin levels were determined by radioimmunoassay using this antiserum and racemic [14C]warfarin and by fluorometric assay after isolation by thin layer chromatography. After a single administration of warfarin (2 mg kg-1 orally or 500 microgram kg-1 i.v.), the plasma levels determined by both assay methods showed a good correlation (r = 0.97, P less than 0.001, Y = 1.04-0.09). The results show that the radioimmunoassay can determine total plasma warfarin without interference of ...

Radioimmunoassay for ovine, caprine and bovine prolactin in plasma and tissue extracts: 1. A radioimmunoassay for ovine prolactin is described based on the inhi

A radioimmunoassay with specificity for the a1 allotype determinant of rabbit immunoglobulin (Ig) heavy chains was used for the quantitation of VH in detergent extracts of lymph node cells from a1a1/b4b4 rabbits. B cell-derived Ig in the same extracts was estimated with a radioimmunoassay specific for L-chain. About 2 X 10(5) molecules/cell was found by both assays when assuming a molecular weight of 1.5 X 10(5) dalton. Extracts prepared from lymphocytes depleted of B cells (surface Ig+ cells) were also found to contain corresponding amounts of a1 and L-chain, with values varying from about 10(3) to 10(4) molecules/cellmthe estimates were not influenced by the presence of inhibitors of proteolytic enzymes. Supernatants, obtained after keeping the lymphocytes in culture for 16 h, were analyzed by the same methods. Again, matching amounts of a1 and L-chain were found in supernatants from nonfractionated as well as from B cell-depleted populations. The results obtained indicate that T cells do not ...

Davies P, Katzman R, Terry RD. Reduced somatostatin-like immunoreactivity in cerebral cortex from cases of Alzheimer disease and Alzheimer senile dementa ...

Introduction: Chromogranin A (CgA) is not very accurate for the diagnosis of gastric carcinoid type1 (GC1). Clinical interpretation of CgA results may be affected by the heterogeneity between CgA assays. The commercial CgA assay, DAKO (DAKO, Denmark A/S, Glostrup, Denmark) is an ELISA which recognizes a 23 kD C terminal fragment of CgA; the Imperial Supra-regional Assay Service radioimmunoassay (SAS Hammersmith Hospital, Imperial College, London) is a competitive radioimmunoassay raised against the whole pancreastatin molecule ...

Blood levels should be monitored 2 to 3 times during the first few weeks of therapy or if the route of administration is changed (Draxler & Canafax, 1988; Evans et al, 1986). After several months, stable patients can be monitored less frequently. After cyclosporine has reached steady state, trough concentrations yield the most useful information for therapeutic monitoring (Evans et al, 1986). The therapeutic range of cyclosporine levels vary according to the assay technique used. The polyclonal radioimmunoassay (RIA) method is less specific for cyclosporine than high performance liquid chromatography (HPLC). Metabolites of cyclosporine are also detected by polyclonal RIA. Polyclonal RIA may be faster and easier to use than HPLC. Monoclonal RIA is more specific for cyclosporine than monoclonal RIA; however, it has not been used clinically as commonly as the other two methods. Cyclosporine levels should be monitored in whole blood because cyclosporine is significantly bound to the cellular ...

Uotila, M; Engvall, E; and Ruoslahti, E, Monoclonal antibodies in radioimmunoassay of alphafetoprotein. Abstr. (1980). Subject Strain Bibliography 1980. 2317 ...

Antigen-Antibody-Reactions, Antigens, Binding-Sites, Castration, Cattle, Estrus, Female, FSH, Goats, Immune-Sera, Iodine-Isotopes, LH, Male, Mice, Pregnancy, Rabbits, Radioimmunoassay, Rats, Serum-Albumin, Testis: ph. ...

Reuter, A M ; Ketelslegers, Jean-Marie ; Hendrick, Jean-Pierre ; Franchimont, P.. Radioimmunoassay of protein hormones: principles and methodology.. In: Hormone research, Vol. 9, no. 6, p. 404-21 (1978 ...

In 21 normal fasting subjects basal serum-pancreatic-polypeptide (H.P.P.), as measured by a specific radioimmunoassay, was 43 +/- 4 pmol/l (mean +/- S.E.M). This basal concentration was of the same magnitude as that of other gastrointestinal peptide hormones. In normal subjects the H.P.P. response t …

[113 Pages Report] Radioimmunoassay Market report categories the Global market by Application (Research, Clinical Diagnostics), Type (Reagents & Kits, Analyzers), End User (Hospital, Pharmaceutical Industry, Academics, Clinical Diagnostic Labs) & Geography

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The U937 cell line has been shown to generate tumour fibrosis factor α (TNF-α) in response to soluble stimuli such as PMA and LPS, but only after treatment with GM-CSF. We report here the generation of TNF-α from U937 cells following phagocytosis of opsonised zymozan particles without the need for pre-treatment with GM CSF. The release of TNF-α from U937 cells was demonstrated by a specific radioimmunoassay, L929 cell killing and neutrophil priming. The biological activities in the cell supernatant were inhibited by TNF-α affarserum. Phagocytosis was required for TNF-α production. Non-opsonised zymozan or latex particles which were not phagocytosed or pretreatment with cytochalasin B, which inhibited phagocytosis of opsonised zymozan particles, all failed to trigger TNF-α production. Phagocytosis failed to trigger detectable IL-1 generation, and production of IL-6 was insufficient to produce biological effects on neutrophils. The U937 supernatant thus provides a source of human TNF-α ...

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TY - JOUR. T1 - Plasma digoxin monitoring is best practice. AU - Tormey, William. PY - 1998. Y1 - 1998. M3 - Article. VL - 91. SP - 142. JO - IRISH JOURNAL OF MEDICAL SCIENCE. JF - IRISH JOURNAL OF MEDICAL SCIENCE. SN - 0021-1265. ER - ...

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Previously, heterozygous INS gene mutations had been detected in adult patients with so-called familial hyperinsulinemia or hyperproinsulinemia who presented with variable phenotypes (mild diabetes or even hypoglycemia) and high serum levels of radioimmunoassayable insulin or proinsulin-like material. More recently, INS mutations have been found to be associated with neonatal- and infancy-onset diabetes (1-3). We demonstrated that mutant insulins with proteotoxic effect cannot be secreted when expressed in HEK 293 cell line (1), and it is likely that SB8 and SSignal23 are also retained in the endoplasmic reticulum. Nevertheless, the patient bearing the mutation in the signal peptide shows a milder clinical course, and we cannot exclude that SSignal23 preproinsulin may be partially processed and secreted. Present knowledge indicates that insulin mutations with a proteotoxic effect cause apoptosis of the pancreatic β-cell (1), a process that in most patients takes several months after birth (1-3) ...

[Testing Process] This kit employs Double Antibody Sandwich Technique. The principle of Double Antibody Sandwich is based on characteristics of the tested antigen with more than two valances which can identify coated antibody and detection antibody...

Invitrogen Anti-ApoA1 Monoclonal (6001), Catalog # MIA1401. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP), ELISA (ELISA) and Radioimmune assays (RIA) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 1 mg purified antibody (5 mg/mL).

کیت سنجش PSA به روش رادیوایمونواسی نام تعیین کمی غلظت PSA به روش رادیوایمونواسی در سرم انسان نام کامل کیت Cancer RIA kits نام دسته 100 تعداد تست Immun...

Definition : Radioimmunoassay analyzers that require the human manipulative effort (e.g., manual insertion of reagents and samples) in all steps of each test, including manual pipetting, control of incubation, separation, and counting. These tests are usually performed using manufacturers kits, which include the unlabeled and radioactively labeled ligands, the binder, and the calibration standards. Most current manual radioimmunoassay analyzers include computerized data-processing (data reduction) devices.. Entry Terms : Radioimmunoassay Analyzers, Manual. UMDC code : 16865 ...

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This protocol can be used to measure the concentration of steroid hormones in small plasma samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington. It has been written for use in the laboratory of Dr. Ellen Ketterson at Indiana University, and therefore the details may require modification if the procedure is performed elsewhere. The general procedure, which takes 4-5 days to complete, is described below. Prepared January 1998 by Joe Casto, modified December 2005 by Jodie Jawor and May 2010 by Danielle Whittaker ...

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INTRODUÇÃO: A mortalidade das vítimas de queimaduras tem diminuído nos últimos anos e a lesão inalatória tornou-se a principal causa de morte. As consequências da inalação por fumaça resultam de uma resposta inflamatória que envolve vários mediadores. A proteína 1 relacionada a uteroglobulina (UGRP1) é uma secretoglobulina antinflamatória específica do pulmão que pode desempenhar um papel na inflamação pulmonar. OBJETIVO: Investigar se a expressão precoce da UGRP1 na inalação de fumaça está relacionada com a severidade da injúria inalatória. MÉTODOS: Nós estudamos 16 pacientes críticos vítimas de queimaduras. Todos os pacientes foram mecanicamente ventilados por Síndrome da Angústia Respiratória do Adulto (SARA) secundária a injúria por inalação de fumaça. Uma amostra de sangue foi coletada entre a admissão a UTI até 24h após a injúria inalatória. O grau da injúria por inalação foi avaliado em todos os pacientes. RESULTADOS: A média de idade dos ...

Principal Investigator：HASEGAWA Yoshihisa, Project Period (FY)：1989 - 1991, Research Category：Grant-in-Aid for Developmental Scientific Research., Research Field：畜産学(含草地学)

Колхицин Bremner W. J., Paulsen С. A. Colchicine and testicular function in man. - New Engl. J. Med., 1976, 294, 1384.Ertel M. H., Miller J. C, Akgun S., Wallace S. L. Radioimmunoassay for colchicine in plasma and urine. - Science, 1976, 193, 233.Goldfinger S. E. Colchicine for familial Meditarranean ...

Thank you for your interest in spreading the word about Science.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...

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The World Health Organization estimates that worldwide 25 million people have cancer and that over 10 million new cases are diagnosed each year. Cancer stage at

Treatment of cattle with sex steroids increases lean muscle mass, accelerates the rate of growth, and is an efficient way to increase meat yield. Estrogens are usually given by implantation of estrogen pellets into the skin on the underside of the ear, and the ear is discarded during slaughter. Unlike GH, sex steroids are not species-specific and may be given orally without degradation in the stomach. In 1998, the Food and Agriculture Organization of the United Nations and World Health Organization jointly concluded that meat from estradiol-treated animals was safe on the basis of data obtained from residue levels in meat from studies performed in the 1970s and 1980s using radioimmunoassay methods. One study demonstrated concentrations of estrogens found in meat residues were low and overlapped with concentrations found in untreated cows.34 Gas chromatography measurements of sex steroids progesterone, testosterone, 17β estradiol, and estrone and their metabolites in meat products, fish, ...

Comprehensive supplier list for 2nd Antibody Sep. Goat Antibody Digoxin (125-I) Radioimmunoassay,2nd Antibody Sep. Goat Antibody Digoxin (3-H) Radioimmunoassay

Cultures of minced, whole-ovary (whole-ovary culture) were used to determine if three selected chemicals altered steroidogenic profiles. First, phenolsulfonthalein (PST), when used in culture medium, was tested for its influence on in vitro steroidogenesis. Next, aminoglutethimide (AGTP; 0 or 150 mg/kg once) and di(2-ethylhexyl)phthalate (DEHP; 0 or 1500 mg/kg/day for 10 days) were administered in vivo to young adult cycling rats, and the ovaries and adrenals were removed and cultured for 1 h. Ovarian steroidogenic profiles of progesterone (P), testosterone (T), and estradiol (E) release into the medium were measured using radioimmunoassay techniques. PST in medium significantly altered. Therefore, PST was excluded in the later studies. DEHP altered steroid profiles so that proestrus appeared to be delayed. AGTP decreased P and E production significantly, and T production was increased slightly in proestrus ovaries. These AGTP alterations in T and E resulted in a highly significant increase in ...

TY - JOUR. T1 - Calcium-dependent intracellular degradation of parathyroid hormone. T2 - A possible mechanism for the regulation of hormone stores. AU - Habener, Joel F.. AU - Kemper, Byron. AU - Potts, John T.. PY - 1975/8. Y1 - 1975/8. N2 - The dynamics of parathyroid hormone (PTH) biosynthesis, storage, and secretion in bovine parathyroid slices in vitro in response to alterations in the concentrations of extracellular calcium were studied. Hormone biosynthesis was evaluated by using polyacrylamide gel electrophoresis to measure incorporation of [3H]leucine into newly synthesized PTH and proparathyroid hormone (ProPTH) during short (35 min) incubations. Amounts of newly synthesized PTH stored in and secreted from the tissue slices were determined by electrophoretic analysis of [3H]PTH in extracts of tissue and media. Total PTH and ProPTH is slices and media were measured by specific radioimmunoassays. PTH secretion rates changed 5-fold when calcium was lowered from 2 mM to 1 mM. Secretion of ...

in Clinical and Experimental Rheumatology (1999), 17(2), 151-60. OBJECTIVES: The aim of this study was to investigate the effects of two nonsteroidal anti-inflammatory drugs (NSAIDs), nimesulide and sodium diclofenac, on the production of proteoglycans (PG ... [more ▼]. OBJECTIVES: The aim of this study was to investigate the effects of two nonsteroidal anti-inflammatory drugs (NSAIDs), nimesulide and sodium diclofenac, on the production of proteoglycans (PG), prostaglandin E2 (PGE2) and cytokines (IL-6 and IL-8) by human articular chondrocytes in vitro. METHODS: Enzymatically isolated chondrocytes were cultured under constant agitation in a well defined culture medium. Specific radioimmunoassays were used to quantify PG and PGE2 production. Cytokine production (IL-6 and IL-8) was assayed by enzyme amplified sensitivity immunoassays (EASIAs). RESULTS: At a concentration of 3 micrograms/ml, nimesulide did not affect the PG production by chondrocytes. This concentration was superior to the ...

TY - JOUR. T1 - Clinical validation of an RIA for natural and recombinant erythropoietin in serun and plasma. AU - Goldberg, Mark A.. AU - Schneider, Thomas J.. AU - Khan, Shaista. AU - Petersen, John R.. PY - 1993. Y1 - 1993. N2 - A sensitive radioimmunoassay (RIA) for the detection of erythropoietin (EPO) was developed using antibody directed against EPO from human urine. With 100 μL of sample, the assay is sensitive to 7 U/L, well below the mean EPO level in normal males (15.1 ± 3.5 U/L) or females (15.4 ± 4.8 U/L). Dilutions of a variety of human serum samples show a parallel relationship with the standard EPO. Clinical validation of the RIA was confirmed by appropriate increases or decreases of EPO levels in various types of anemia and polycythemia. Serum EPO levels were also measured in volunteers participating in an autologous blood donation study. The RIA proved to eb quite sensitve, detecting small increases even after a single unit phlebotomy. This RIA of human EPO meets all the ...

TY - JOUR. T1 - Properties of endogenous somatostatin like immunoreactivity and synthetic somatostatin in dog plasma. AU - Conlon, J. M.. AU - Srikant, C. B.. AU - Ipp, E.. AU - Schusdziarra, V.. AU - Vale, W.. AU - Unger, Roger H. PY - 1978. Y1 - 1978. N2 - Somatostatin-like immunoreactivity (SLI) in the peripheral venous plasma of dogs and in their pancreatic and gastric venous effluents was characterized and compared with synthetic somatostatin. Both endogenous plasma SLI and somatostatin added to plasma were eluted from Sephadex gels at pH 8.8 in the 150,000-200,000-mol. wt region but at pH 2.5 both appeared in the 1,500-2,000-mol. wt region. The SLI released from the isolated dog pancreas perfused with plasma-free buffer was eluted entirely as a 1,600-dalton polypeptide, but when the pancreas was perfused with plasma, SLI was eluted in the 150,000-200,000-mol. wt zone. Affinity chromatography of plasma samples on immobilized antibodies directed against the central portion of the ...

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During the past 30 years, many methods have been developed for determining vitamin D status in humans (5)(6)(7)(8)(9). These techniques have involved competitive protein-binding assays (5), direct UV detection after HPLC (6)(9), and RIA (7)(8). The 125I-based RIA has emerged as the method of choice for the determination of circulating 25(OH)D. The clinical use and utility of this marker for calcium homeostasis greatly increased in 1992 after a report that demonstrated a relationship between circulating concentrations of 25(OH)D and hip fracture incidence (2). Today, the determination of circulating 25(OH)D is a common clinical event. To that end, there currently are two FDA-approved devices in use clinically for the detection of circulating 25(OH)D in the United States. These methods were both evaluated in this study.. In the determination of nutritional vitamin D status, it is imperative that the method of choice measure circulating 25(OH)D2 and 25(OH)D3 equally to provide total circulating ...

The choriogonadotropin beta-subunit radioimminoassay has been used extensively to measure human choriogonadotropin in the sera of pregnant women and individuals with trophoblastic and nontrophoblastic tumors. Unmodified, this method cannot be used to measure choriogonadotropin in urine because of interfering substances. We circumvented the non-parallelism between the standards and serial dilutions of urine containing choriogonadotropin by adding pooled urine from men to the standard tubes and limiting the volume of urine to 100 microliter. This modified assay has a sensitivity of 3 int. units/liter of urine and is specific for choriogonadotropin concentrations of 40 int. units/liter of urine. Analytical recovery of choriogonadotropin added to urine ranged from 96 to 105%. The within-assay CV was 7.6%; the between-assay CV was 11.8%. Concentrations of choriogonadotropin in concurrently collected serum and urine samples from pregnant women correlated well. The test can be performed within 24 h by ...

Hormone-free serum is required for use as a matrix for standards in the radioimmunoassay of hormones. The glycoprotein hormones are difficult to remove from serum by conventional techniques. We exploited the specific binding of carbohydrates by lectins to extract glycoprotein hormones from human serum. Passing serum over Concanavalin A Sepharose efficiently removed lutropin and the beta subunit of choriogonadotropin. Wheat-Germ Lectin Sepharose completely removed these, and also follitropin and thyrotropin. The latter treatment is shown to provide a suitable matrix for standards in radioimmunoassays of the four hormones.

en] A radioimmunoassay of the undecapeptide substance P (SP) and its application in rabbit lung superfusate has been developed. The assay was based on the use of a radioactive tracer, 125I-SP-Tyr8, which showed, with an excess of antibodies, a specific binding greater than 90% and a nonspecific binding lower than 2%. This tracer was stable for 4 weeks at -20 degrees C. Its specific radioactivity was 384 Ci/mmol. The assays lower limit of detection was 10 pg/ml (0.7 fmol). The determination of SP in rabbit lung perfusate required extraction and concentration using octadecylsilane cartridges. Under these conditions, the recovery of SP from experiments on lung perfusates (n=4) was 75.0+/-4.5%, and the intraassay and interassay coefficients of variation were 7.3+/-1.9% and 10.7+/-1.3%, respectively. Amounts of SP released in rabbit lung perfusates were determined following stimulation of pulmonary C fibers with capsaicin (10(-4) M). During the stimulation period, the values of SP from lung ...

OBJECTIVES: I. Compare growth hormone (GH) levels at baseline and after glucose suppression measured with both a polyclonal radioimmunoassay and a highly sensitive immunoradiometric assay (IRMA) in patients with acromegaly and normal volunteers.. II. Measure the levels of IGF-I and its binding protein, IGFBP-3, in these cohorts.. III. Determine any correlation between levels of IGF-I and IGFBP-3 and GH suppressibility as assessed by sensitive IRMA.. IV. Determine if patients who demonstrate biochemical features of mild GH excess are at risk for progression to active disease. ...

OBJECTIVES: I. Compare growth hormone (GH) levels at baseline and after glucose suppression measured with both a polyclonal radioimmunoassay and a highly sensitive immunoradiometric assay (IRMA) in patients with acromegaly and normal volunteers.. II. Measure the levels of IGF-I and its binding protein, IGFBP-3, in these cohorts.. III. Determine any correlation between levels of IGF-I and IGFBP-3 and GH suppressibility as assessed by sensitive IRMA.. IV. Determine if patients who demonstrate biochemical features of mild GH excess are at risk for progression to active disease. ...

Radioimmunoassay is used primarily in the healthcare industry due to its high sensitivity, which can be used to analyze nanomolar and picomolar concentrations of hormones in biological fluids. RIA has ever since becoming one of the major tools in various clinical laboratories for diagnostic procedures, such as assessing the plasma levels of most of the hormones, identifying the presence of certain abused drugs, checking for the presence of hepatitis B surface antigen in donated blood, and other similar applications.

The biologic and clinical significance of the oncofetal antigens carcinoembryonic antigen (CEA) and alpha1-fetoprotein (AFP) are discussed. Although the current assays for these molecules are not tumor-specific, measurement of these molecules in the circulation of cancer patients is useful either for tumor diagnosis or for management of the cancer patient in the postoperative or post-chemotherapy state. An approach to increasing the specificity of the CEA radioimmunoassay is described. ...

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BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.

BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.

hCG alpha-5 Monoclonal Antibody from Invitrogen for Western Blot, ELISA and Radioimmune Assays applications. This antibody reacts with Human samples. Clone: INN-hFSH-158. Supplied as 250 µg purified antibody (1 mg/ml) in PBS with 0.09% sodium azide.

UCL Discovery is UCLs open access repository, showcasing and providing access to UCL research outputs from all UCL disciplines.

UCL Discovery is UCLs open access repository, showcasing and providing access to UCL research outputs from all UCL disciplines.

M pore size; Amersham Biosciences). Immunodetection was performed with either a monoclonal rabbit anti-human SAP antibody (1:5,000; Cat. no. 1793-1; Epitomics,

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Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...

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Most employers and organizations are moving from conducting urine drug tests to saliva testing or hair drug test since these are considered to be more

Products for in vitro diagnosis of viral infectious disease by detection of antibodies, antigens and proteins by ELISA and Immunofluorescence in addition to molecular biological amplification techniques for identification and detection of viral nucleic acids. ...

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO s term and conditions which are available at www.exbio.cz ...

Monobind makes clinical diagnostics that are innovative, simple to use, cost effective and vital for human-care. Since 1978, it provided 200+ million immunoassays ranking among the largest IVD producers.

• Sufficient for 100s of Samples • • One Drop Per 5 mL of Blood • For Blood Preservation • Sequester-Sol® is an effective, non-toxic anticoagulant, widely used in hematology and blood chemistry procedures. And its very convenient and economical to use. It stabilizes the cellular constituents of blood in the same mann

At Terumo Interventional Systems, we constantly work to refine and perfect our products so that interventionalists can do more. We are proud to support great thi

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Evaluation of chosen heavy metals and enzyme exercise in soils throughout the zone of affect of assorted tree species The research aimed to guage the overall content material and bioavailable types ...

Supplementary MaterialsSupplementary materials 1 (PDF 45 kb) 262_2012_1263_MOESM1_ESM. Outcomes KLH-specific IgM, IgA, IgG and everything IgG-subclasses were measured in concentrations only 20 accurately?g/ml. The intra- and inter-assay coefficients of deviation of the ELISA had been below 6.7 and 9.9?%, respectively. Analyses of 128 sufferers demonstrated that older DC induced higher degrees of KLH-specific IgG in […]. ...

If there is a need to give a stress dose of steroids immediately due to clinical instability but there is a plan to still test for AI after by measuring serum cortisol, giving dexamethasone may be a good choice of steroid, as it is not measured in the assays for serum cortisol (hydrocortisone will interfere with the test and is measured in the cortisol radioimmunoassay ...

Buy HP Urease recombinant protein (MBS8506687) product datasheet at MyBioSource, Recombinant Proteins. Application: ELISA (EIA), CLIA, Radioimmunoassay (RIA), immunohistochemistry (IHC), Western Blot (WB)