TY - JOUR. T1 - Human prostatic acid phosphatases. II. A double-antibody radioimmunoassay. AU - Choe, B. K.. AU - Pontes, E. J.. AU - Morrison, M. K.. AU - Rose, N. R.. PY - 1978. Y1 - 1978. N2 - A double-antibody radioimmunoassay method for prostate-specific acid phosphatase (PAP) is presented. Experimental details are outlined to assess the reproducibility and reliability of the method under various assay conditions. The upper limit of the serum PAP levels in the present assay was set at 2.4 ng/100 μl by 162 determinations of normal serum samples. The serum PAP levels of patients with nonprostatic malignant tumors fell in the normal range, whereas the levels higher than 4.0 ng/100 μl were found in patients with prostatic carcinoma.. AB - A double-antibody radioimmunoassay method for prostate-specific acid phosphatase (PAP) is presented. Experimental details are outlined to assess the reproducibility and reliability of the method under various assay conditions. The upper limit of the serum ...

Adenosine was coupled to human serum albumin by two different procedures that preserved the purine and ribose rings. The conjugates were evaluated for antigenicity in rabbits and guinea pigs. A conjugate containing 2′(3′)-O-succinoyladenosine failed to elicit antibodies, whereas one containing laevulinic acid (O2′, 3′-adenosine-acetal) elicited antibodies in all animals injected. The affinity and specificity of binding of adenosine to three selected antisera were evaluated. Dissociation constants of 31-187 nM were observed. Displacement of adenosine binding to all antisera by adenine 5′-nucleotides, adenine, inosine and hypoxanthine required more than 1000-fold higher concentrations than of adenosine itself. Similar affinities for adenosine and 2′-deoxyadenosine were observed. By exploiting the high specificity of the antisera, a radioimmunoassay method was established that was capable of detecting down to 1 pmol of adenosine (20 nM) in unfractionated heart perfusates and cell ...

Law, Brian (1983) RADIOIMMUNOASSAY METHODS FOR THE FORENSIC ANALYSIS OF CANNABINOIDS IN BIOLOGICAL FLUIDS Doctoral thesis, University of Surrey. ...

Current immunoassays for interleukin-1 beta (IL-1 beta) are effective for analyzing fluids derived from cultured cells. However, IL-1 beta determinations in human plasma or serum samples are technically complicated by higher protein and lipid concentrations, physicochemical differences which exist between samples from healthy subjects and those experiencing acute phase responses, and by the fact that IL-1 beta can be produced and degraded in the blood collection tube after the sample is drawn. A simple chloroform extraction process has been developed which eliminates several of the interfering factors from plasma samples and increases the amount of IL-1 beta detected by radioimmunoassay and lymphocyte activation assay. In the radioimmunoassay, rabbit sera was found to influence the accuracy and variability of plasma measurements. Improvements in radioimmunoassay reagents and methods are reported which reduce this influence. Finally, different concentrations of IL-1 beta were measured depending on

This paper describes a rapid sensitive solid phase radioimmunoassay procedure for staphylococcal enterotoxin B, which can be applied to A and C. The procedure is 100-fold more sensitive thandescribed immunodiffusion and precipitin methods and is reliable at a level of 0.01 µg/ml. It is usable for detection ad measurement of enterotoxin in foodstuffs, biologic fluids and toxin preparations, both purified and crude.. ...

Background: PYY is a gut hormone that physiologically inhibits appetite. Currently only radioimmunoassay (RIA) techniques are available for its estimation. The aim of the study was to evaluate our in-house assay for precision, detection limit, interference of haemolysis, suitability of serum versus plasma and biological variation within one subject.. Method: All samples were obtained from one subject on two occasions following an over night fast and the consumption of 1000 and 3000 kcal meals on the respective days. Plasma was aliquoted in multiple samples. Coefficient of variation (CV) was measured at three concentrations (13.4, 16.4, 31.7pmol/L). The influence of haemolysis was evaluated by diluting severely haemolysed blood with non haemolysed blood obtained at the same venepuncture. All plasma samples were obtained in lithium heparin and trasylol tubes. Serum samples were obtained in a tube with trasylol and a clot activator. Carry over and the detection limit of the assay were also ...

The aim of the current study was to describe the use of a pool of different antisera raised against pregnancy-associated glycoproteins (PAGs; purified from both ovine and caprine placentas) for early pregnancy diagnosis in ovine species. Sixty-three

Polyspecific antiserum against total rat serum proteins was used to develop specific and sensitive radioimmunoassays for fetuin and bikunin, two minor protein components of rat plasma. The radioimmunoassays proved to be highly useful to trace bikunin and fetuin in the course of developing isolation procedures, since neither specific functional assays nor monospecific antisera were available. The two examples demonstrate that, in general, it will be possible to develop a specific and sensitive radioimmunoassay with antiserum raised against a crude antigen preparation, such as a body fluid or a tissue extract, provided that a minute amount of pure antigen is available for preparing the radioiodinated antigen. ...

E. Piall, N. Curtin, W. Aherne, A. Harris, V. Marks; The Quantification of 2′-Deoxyuridine 5′-Triphosphate in Anti-Folate Treated Cells using a Sensitive Radioimmunoassay. Clin Sci (Lond) 1 January 1987; 73 (s17): 18P. doi: https://doi.org/10.1042/cs073018Pa. Download citation file:. ...

TY - JOUR. T1 - Detection of interleukin 1α and 1β in rabbit tissues during endotoxemia using sensitive radioimmunoassays. AU - Clark, B. D.. AU - Bedrosian, I.. AU - Schindler, R.. AU - Cominelli, F.. AU - Cannon, Joseph Gerard. AU - Shaw, A. R.. AU - Dinarello, C. A.. PY - 1991/12/1. Y1 - 1991/12/1. N2 - Interleukin 1 (IL-1) is a primary mediator of a wide variety of immunologic and inflammatory responses, including reactions to microbial infections. To study this cytokine in an animal model, we have developed specific and sensitive radioimmunoassays for the quantitation of rabbit IL- 1α and IL-1β. The sensitivity (limit of detection at 95% confidence level) of our assay for IL-1α and 1β was 20-40 and 40-80 pg/ml, respectively. Recovery of IL-1 from tissues ranged from 75 to 107%, with a mean of 95% for IL-1α and 89% (range 19-98) for IL-1β. We employed these assays in in vivo and in vitro studies. In an in vivo model, we measured the amount of rabbit IL-1α and 1β protein present in ...

The involvement of central catecholaminergic neurons in tonic gonadotrophin release was investigated. Male rats were given a single cerebral intraventricular injection of 6-hydroxydopamine hydrochloride (6-OHDA; 170 μg free base) dissolved in 0·001 n-HCL. Plasma LH and FSH were measured by double antibody radioimmunoassay. In the 6-OHDA-treated animals, LH was significantly reduced (P,0·001 after 1 hr and remained consistently lower for 8 hr. After a transient elevation, the controls showed no significant change (P,0·01). No difference in LH concentrations was found between experimental groups sampled at 2 days or later (P,0·01). Levels of FSH were less consistently different. Administration of 6-OHDA dissolved in different vehicles, with varied pH and osmolarity, gave similar results which suggests that the quinone derivative of 6-OHDA is effective in producing the catecholaminergic impairment. These findings indicate that LH release in the male rat may be controlled (or at least ...

The pneumococci possess, in addition to type-specific capsular polysaccharides, a number of antigens common to the species. Antibodies to phosphocholine (PC), a major determinant of C-carbohydrate, have been shown to protect mice from experimental pneumococcal infection, but little is known of the role of anti-PC antibodies in humans or the extent to which anti-PC levels are affected by carriage or infection. We examined 115 sera from 30 infants, who were followed propsectively from birth through 4 years of age, for the presence of immunoglobulin M antibody to PC, using a solid-phase radioimmunoassay method. Infants were found to develop antibody to PC in response to pneumococcal carriage and infection, and nearly all infants developed some antibody. Antibody levels increased with age. By using a regression model including both age and nasopharyngeal carriage of pneumococci, anti-PC levels were found to be highest after exposure to two or three different types of pneumococci; levels were highest ...

TY - JOUR. T1 - Central obesity and hypertension: the role of plasma endothelin. Am J Hypertens. AU - Licata, Giuseppe. AU - Corrao, Salvatore. AU - Scaglione, Rosario. AU - Parrinello, Gaspare. AU - Licata, Anna. AU - Pinto, Antonio. AU - Parrinello, Gaspare. PY - 1996. Y1 - 1996. N2 - Hypertension and central obesity are two conditions closely linked, but the mechanisms responsible for obesity-associated hypertension are still unclear. In the last few years, several studies addressed the role of endothelin-1 (ET-1) in the development and maintenance of hypertension. This study was designed to evaluate plasma ET-1 in normotensive and hypertensive central obese subjects compared with a lean healthy group. Our final goal was to analyze the relationship between plasma ET-1, blood pressure, and left ventricular structure and function in central obese subjects (both normotensives and hypertensives). ET-levels have been assessed by the radioimmunoassay method in 20 lean normotensives and in 57 ...

Summary. Baboon CG from the urine and placenta (Days 33-45 of gestation) was purified by ammonium acetate/alcohol extraction followed by ionic exchange, gel filtration and affinity chromatography. The CG activity was monitored using a double-antibody radioimmunoassay utilizing anti-baboon CG and hCG both as the labelled ligand and standard. Biological activity was measured by the rat luteal cell radio-receptor assay. The purified preparation exhibited heterogeneity in terms of its behaviour during ionic exchange chromatography and isoelectric focussing. Like hCG, baboon CG was made up of two non-covalently linked subunits: the Stokes radii were 36·5, 29·0 and 22·0 × 10−10 m for native CG, the beta subunit and the alpha subunit. The material focussed between pH 4·2 and 5·5. Relative to the second international hCG standard the biological potency of the purified urinary baboon CG was 4058 i.u. whilst the immunological activity was 4364 i.u. It is concluded that baboon and human CG are ...

The Medical Journal of the Islamic Republic of Iran (MJIRI), is a quarterly and an editorially independent publication owned by Iran University of Medical Sciences and aims to be a publication of international repute for reporting current regional and international adventures in all aspects of the medicine. MJIRI welcomes contributions in this field in the form of original research articles, case reports, letter to the editor, review articles, brief communications, debate articles, and study protocols.

A solid phase radioimmunoassay for adrenal antibodies is described. In the assay plastic tubes coated with adrenal microsomes (100 micrograms/ml) were incubated with human sera diluted from 1:50 to 1:5000 and the retained antibodies detected by subse

p,Serum specimens from 12 patients with type A hepatitis were analyzed for immunoglobulin M-type antibody to hepatitis A virus (IgM anti-HA). A recently developed solid-phase radioimmunoassay kit for IgM anti-HA (HAVAB-M, Abbott Laboratories) and a competitive binding radioimmunoassay kit (HAVAB, Abbott Laboratories) with or without 2-mercaptoethanol treatment, as modified by Yano et al. (Acta Hepatol. Jpn. 21, 704-712, 1980) were used to obtain an M-index. All specimens obtained within 60 days of the onset of illness and specimens from 2 of 4 patients later than 60 days after the onset were positive with the HAVAB-M test. This test gave negative results to sera which were positive for anti-HA by a standard HAVAB test in the following: 3 patients with type B hepatitis; 5 with non-A, non-B hepatitis; 11 healthy adults; and 10 sera strongly positive for rheumatoid factor. The M-index for type A hepatitis in sera within 30 days of the onset (mean value of the M-index, m, = 1.52; standard deviation, ...

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Thank you for your interest in spreading the word about Biochemical Journal.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...

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Evidence for a Nonspecific Factor Interfering in the Radioimmunoassay of Somatoliberin-Like Immunoreactivity in Human Seminal ...

Horn, K.; Blümel, K. R.; Koeppen, D. und Scriba, Peter Christian (1974): Radioimmunoassay for T3 in serum: Necessity of prior extraction. 20.Symposion der Deutschen Gesellschaft für Endokrinologie, February 27-March 2, 1974, Tübingen. ...

BioAssay record AID 302415 submitted by ChEMBL: Inhibition of COX2 in LPS-stimulated J774 cells assessed as inhibition of PGE2 levels at 1 uM by radioimmunoassay.

Verstraeten, A A.; Hageman, P C.; and Kwa, H G., "A radioimmunoassay for mtv-antigens." (1973). Subject Strain Bibliography 1973. 342 ...

Follow assay procedures as outlined in USP1 or AOAC.2 Use levels of B12 in the preparation of the standard curve according to these references. It is essential that a standard curve be constructed each time an assay is run. Autoclave and incubation conditions can influence the standard curve reading and cannot always be duplicated. Generally satisfactory results are obtained with B12 at the following levels: 0.0, 0.025, 0.05, 0.075, 0.1, 0.125, 0.15, 0.2 and 0.25 ng per assay tube (10 mL ...

By the 1970s, there were already many examples of how radioimmunoassays improved diagnostic laboratory methods. Although mildly disadvantaged because of the need for special conditions related to the...

The Ordinary Buffet Serum Value Size - The Ordinary Buffet Serum is a multi-technology peptide serum.  This formula combines a compre

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According to recent investigations neopterin (a pyrazinopyrimidine derivative) is a biochemical marker that reflects the activity of the proinflammatory immunocellular system of the synovial tissue in rheumatoid arthritis (RA). Interferon gamma, derived from antigen activated T lymphocytes, stimulates macrophages to synthesise and release neopterin into the culture supernatant in vitro. To extend this in vitro model to a clinical level a sensitive new radioimmunoassay technique was used to measure neopterin concentrations in the synovial fluid (SF) of 17 patients with active RA, nine with osteoarthritis, and six with acute gout, and in that of 12 controls undergoing meniscectomy. The SF neopterin concentrations were significantly higher in patients with RA than in the other groups of patients, particularly the controls. Multivariant analysis showed that SF neopterin concentrations correspond better with the systemic inflammatory activity of RA than with the local disease activity of the knee ...

In this paper a method for the radioimmunological determination of triamcinolone acetonide (TAAc) in plasma is described. The antiserum used in the radioimmunological determinations showed high...

Oestrus was synchronised with PGF2-alpha in four pluriparous West African Dwarf (WAD) goats and blood collected through jugular venupuncture. The length of the oestrous cycle and oestrus duration were observed in three successive cycles while peripheral plasma progesterone and oestradiol-17B levels were determined by the radioimmunoassay technique. The mean oestrous cycle lengths and oestrus duration were 19.4 ± 1.7 d and 33.3 ± 6.6 h, respectively. Progesterone levels were very low during the first four days of the cycle (day 1 = day of oestrous) with the lowest level of 0.3 ± 0.02 ng/ml being recorded on day 2 of the cycle. There was a steady rise in the levels during the cycle until day 15 when the highest level of 2.2 ± 0.05 ng/ml was recorded which was followed by a sharp decline on day 20 (P ,0.05). The profile of oestradiol-17B was erratic during the cycle. Mean maximum and minimum concentrations were recorded on day 1 (152.6 ± 31.6 ng/ml) and day 2 (58.8 ± 22.6 ng/ml), ...

Huge collection of Rosalyn S. Yalow quotes. Born on 1921-07-19 Died on 2011-05-30. Nickname: Rosalyn Sussman. Radioimmunoassay, (RIA) Rosalyn Sussman Yalow was an American medical physicist, and a co-winner of the 1977 Nobel Prize in Physiology or Medicine for development of the radioimmunoassay technique. She was the second American woman to be awarded the Nobel Prize in Physiology or Medicine after Gerty Cori.

TY - JOUR. T1 - Bleomycin serum pharmacokinetics as determined by a radioimmunoassay and a microbiologic assay in a patient with compromised renal function. AU - Crooke, Stanley T.. AU - Luft, Friedrich. AU - Broughton, Alan. AU - Strong, James. AU - Casson, Kay. AU - Einhorn, Lawrence. PY - 1977/4. Y1 - 1977/4. N2 - Serum and plasma bleomycin concentrations were determined in a patient with renal dysfunction at two creatinine clearances. The results obtained with a new radioimmunoassay and the microbiologic assay were compared. It was shown: 1) that the clearance of bleomycin from the blood is markedly retarded in severe renal dysfunction, 2) that clearance of bleomycin varies with creatinine clearance, 3) that bleomycin is probably not dialyzable, 4) that determinations on serum and plasma were equivalent, and 5) that the radioimmunoassay and microbiologic assays gave equivalent results (P , 0.001).. AB - Serum and plasma bleomycin concentrations were determined in a patient with renal ...

TY - JOUR. T1 - Endothelium-independent contractions to jvg-monomethyl-l-arginine in canine basilar artery. AU - Katušić, Zvonimir S.. PY - 1991/11. Y1 - 1991/11. N2 - Background and Purpose: NG-substituted analogues of L-arginine are potent and selective inhibitors of nitric oxide synthase(s). The present study was designed to determine the effects of these analogues on the vascular smooth muscle of isolated canine basilar arteries. Methods: Basilar artery rings without endothelium were suspended for isometric tension recording in Krebs-Ringer bicarbonate solution bubbled with 94% O2-6% CO2 (temperature=37°C, pH=7.4). A radioimmunoassay technique was used to determine the levels of guanosine 3,5-cyclic monophosphate (cyclic GMP). Results: NG-Monomethyl-L-arginine (L-NMMA) caused concentration-dependent contractions, whereas the D-enantiomer and NG-nitro-L-arginine did not. Contractions to L-NMMA were reduced in the presence of L-arginine but not in the presence of D-arginine. Superoxide ...

to achieve the student with the theoretical skills that allow an adequate knowledge of physical and biological phenomena at the base of radioimmunoassay techniques.. ...

Figure 2: The Effects of Endogenous Thyroglobulin Antibody on the Measurement of Serum Thyroglobulin using Immunoenzymatic (Sandwich) Method. Thyroglobulin Immunoassays. Several immunoassays are commercially available to measure thyroglobulin including the competitive radioimmunoassay (RIA) and the one-step immunoenzymatic (sandwich) assay (IMA). The RIA method is older and more labor intensive compared to IMA. In the RIA method, thyroglobulin competes with 125I labeled human thyroglobulin for a rabbit antibody against human thyroglobulin. After six days of incubation, thyroglobulin - antibody complexes are precipitated with a second anti-rabbit antibody. This method can detect both free thyroglobulin and thyroglobulin bound to antibody. The radioactivity is inversely proportional to the thyroglobulin concentration (2). In contrast to RIA, the IMA can be performed within one hour. The thyroglobulin in the patients sample is mixed with a biotinylated reagent that contains four monoclonal ...

If indirect immunofluorescence assay (IFA) (ANN1C, ANN2C, ANN3C, PCA1C, PCA2C, PCTRC, AMPHC, CRMC, AGN1C) is indeterminate, then paraneoplastic autoantibody Western blot is performed at an additional charge.. If IFA pattern suggest NMO/AQP4-IgG, then NMO/AQP4-IgG FACS is performed at an additional charge.. If NMO/AQP4-IgG FACS screen assay requires further evaluation, then NMO/AQP4-IgG FACS titration assay is performed at an additional charge.. If IFA patterns suggest CRMP-5-IgG, then CRMP-5-IgG Western blot is performed at an additional charge.. If IFA patterns suggest GAD65 antibody, then GAD65 antibody radioimmunoassay is performed at an additional charge.. If IFA patterns suggest neuronal voltage-gated potassium channel-complex autoantibody, then VGKC-complex antibody IPA is performed at an additional charge.. If VGKCC ,0.00, LGI1-IgG CBA, CSF and CASPR2-IgG CBA, CSF are performed at an additional charge.. If IFA patterns suggest amphiphysin antibody, then amphiphysin Western blot is ...

490 blood samples have been examined for the comparison of the various methods concerned with the detection of the Australia Antigen (Au). The following methods have been comparatively examined: immunodiffusion (ID) counterelectrophoresis (CEP), complement fixation (CF) passive hemoagglutination (HA) and solid phase radioimmunoassay RIA. On the basis of the results so far obtained the RIA appears the most sensitive and specific method, followed by the HA. In order to obtain reproducible results for diagnostic purposes we suggest to carry out routinely two different techniques together (possibly RIA and HA), while for screening purposes just one technique, choosed between the two most indicative ones, shall be employed.

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An immobilized immunoadsorbent for use in immunoassay and radioimmunoassay procedures includes, in a preferred form, a mass of superficially porous refractory particles having a water insoluble polymer, such as dextran, bonded thereto. The polymer in turn has bonded to it, by covalent bonds, antibodies capable of binding a specific antigen thereto and stoichiometrically releasing the bound antigen by the use of a rinsing solution which does not adversely affect the binding of the antibody to the polymer. Preferably, each of the porous particles includes an impervious core which has joined thereto sufficient layers of microparticles to form an outer porous coating on the core. Methods of forming such an immunoadsorbent and for using the same and structure for supporting the same are also disclosed.

The in vivo release of cholecystokinin (CCK)-like material (CCKLM) was measured in the frontal cortex of freely moving rats using the microdialysis technique combined with a sensitive radioimmunoassay. Local perfusion of K+ (100 mM)-enriched artificial CSF resulted in a 10-fold increase in CCKLM outflow, as compared with that occurring under basal resting (K+ = 3.0 mM) conditions, and this effect could be completely prevented by removal of Ca2+ in the perfusing fluid. Chromatographic analyses demonstrated that CCK-8S contributed to 70% of CCKLM. Stressful stimuli such as a 2-min exposure to diethyl ether and a 30-min restraint produced a marked but transient increase in cortical CCKLM release. In addition, anxiety-like behavior induced by the systemic administration of yohimbine (5 mg/kg i.p.) was associated with a long-lasting enhancement in the peptide outflow. Pretreatment with the potent anxiolytic drug diazepam (5 mg/kg i.p., 5 min before each condition), which exerted no effect on its own,

Radioimmunoassay for ovine, caprine and bovine prolactin in plasma and tissue extracts: 1. A radioimmunoassay for ovine prolactin is described based on the inhi

Introduction: Chromogranin A (CgA) is not very accurate for the diagnosis of gastric carcinoid type1 (GC1). Clinical interpretation of CgA results may be affected by the heterogeneity between CgA assays. The commercial CgA assay, DAKO (DAKO, Denmark A/S, Glostrup, Denmark) is an ELISA which recognizes a 23 kD C terminal fragment of CgA; the Imperial Supra-regional Assay Service radioimmunoassay (SAS Hammersmith Hospital, Imperial College, London) is a competitive radioimmunoassay raised against the whole pancreastatin molecule ...

Uotila, M; Engvall, E; and Ruoslahti, E, "Monoclonal antibodies in radioimmunoassay of alphafetoprotein. Abstr." (1980). Subject Strain Bibliography 1980. 2317 ...

Reuter, A M ; Ketelslegers, Jean-Marie ; Hendrick, Jean-Pierre ; Franchimont, P.. Radioimmunoassay of protein hormones: principles and methodology.. In: Hormone research, Vol. 9, no. 6, p. 404-21 (1978 ...