TY - JOUR. T1 - Radiation leukemia virus-induced T-cell lymphomas with common T-cell receptor variable region structure and similar binding specificity for retrovirus. AU - ONeill, H C. PY - 1991/11. Y1 - 1991/11. N2 - The 5C2 cell line was derived following culture of mouse spleen cells exposed in vivo and in vitro to radiation leukemia virus (RadLV) containing supernatants from the C6VL/1 T cell lymphoma. This cell line has been found to express an alpha beta T-cell receptor (TCR) identifiable with the Mab124-40 anti-clonotypic antibody which is specific for C6VL/1. It has been shown to be genetically and phenotypically distinct from C6VL/1 with a unique phenotype, i.e. CD4-, CD8-, CD3+, TCR-alpha beta. 5C2 has been shown to express high levels of alpha and beta chain mRNA and to utilize the same or similar V alpha and V beta region genes as C6VL/1. Whereas C6VL/1 binds cross-reactively to both RadLV/C6VL and an unrelated isolate RadLV/VL3, 5C2 has binding specificity for only RadLV/C6VL, ...

TY - JOUR. T1 - T-cell differentiative capacity of haematopoietic stem cells immortalized in vitro with radiation leukemia virus. AU - Ho, E S. AU - ONeill, H C. PY - 1993/8. Y1 - 1993/8. N2 - The differentiative capacity of a unique haematopoietic cell type has been investigated. These cells have been found to be a common target in vitro to infection and immortalization by radiation leukemia viruses (RadLVs). Many continuous lines of these cells have been generated. Since RadLV retroviruses are known to be strictly T-cell-tropic in vivo, we have questioned whether these cells are precursors of T cells. To this end, the RadLV-induced C1-V13D cell line has been inoculated into thymus of sublethally irradiated syngeneic CBA/H mice and tested for capacity to proliferate and differentiate, i.e. express T-cell markers. When inoculated in high number, C1-V13D cells can induce a thymic tumour within 14 to 21 days. Expression of T-cell markers on these cells was determined by fluorescence-activated ...

Two different classes of neoplastic T cells were isolated from radiation leukemia virus (RadLV)-inoculated and from X-ray-treated C57BL/6 mice. One consisted of growth factor-dependent T-cell lymphoma (FD-TCL) lines which were established from the spleens and thymuses of treated mice within a day of lymphoma detection. FD-TCL cells were often eudiploid and could be grown in pure culture only at high concentrations, or on stromal feeder layers. Non-thymic, factor-dependent TCL cells produced interleukin-2 upon lectin stimulation, and were autostimulatory because they secreted growth factor(s) constitutively. Single cell cloning of FD-TCL cells in semisolid medium required the addition of exogenous conditioned medium. In vivo, FD-TCL cells that were injected intraperitoneally or intravenously homed to the spleen, proliferated in it and killed the injected mice. FD-TCL cells did not produce local tumors at the site of subcutaneous injection. The isolation and study of FD-TCL cells were facilitated ...

We show here that CD8 T cells undergo apoptosis and decline in number in response to IFN and that non-virus-specific bystander CD8 T cells are driven to apoptosis during the T-cell response to virus infections. Apoptosis and cell loss in each of these systems were most pronounced in CD8 T cells of the memory phenotype (CD44hi). IFN-induced attrition and apoptosis of memory cells were confirmed using MHC tetramer analysis of a well-defined LCMV memory population from poly(I:C)-injected LCMV-immune mice (Fig. 4 and Table 3). An early virus-induced peak in T-cell apoptosis and attrition correlated with the peak in virus-induced IFN-α/β, and the IFN-α/β inducer poly(I:C) dramatically induced apoptosis and attrition in the memory CD8 T-cell compartment. Poly(I:C) is a very potent IFN-α/β inducer, but it also induces potentially cytotoxic cytokines like TNF alpha (44). IFN-α/β, in turn, can stimulate the synthesis of a number of other cytokines, such as IL-15 (20, 54), which is known to act on ...

定期的にKIS保護者の皆さんによる給食会が、1月19日（金）のランチタイムに行なわれました。 KISでは、こうした給食会が年に数回行なわれ、生徒や教職員からも大好評です。 この日も朝から準備のために集められた保護者の皆さんがふるまうヤンニョムチキン、豚汁、チヂミ、ご飯に生徒たちの長蛇の列が並びました。 朝早くから準備してくださった保護者の皆さま、本当に有難うございました。. ...

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私は現在の地球上に生きるどこの国の子供達も一人残らず、お腹一杯食べられて、十分な教育を受けられ、互いの国から戦争がなくなる日こそ地球のすべての人間が真に幸福になることだとかねがね思っていました。コリア国際学園の教育の理念と建学の精神は、私の考えに全くぴったりです。特にアジアの子供たちが、こういう教育を受けて伸び伸び自分の才能を伸ばし、たくましく成長していくことには、何という晴れがましい未来をもたらすことかと胸が躍るような気がします ...

We love and respect sports - even if my grandson said: I prefer watch soccer on TV, it is not as tiresome as playing it.. Fishing and Hunting Trade Fairs are becoming very popular. One may say, because we love to be outdoors. Or we can say that Fishing & Hunting are great sports - but ask the rabbit!. F&H trade fairs are often held together and though being patient is common requirement, yet I noticed major differences among the typical visitors. Anglers are more reserved - fishing is a lone fight - just me and the fish - while hunting needs an accord of team. A joint, common experience for hunters - at fairs they are jolly, loud, noisy - and happy together.. Therefore one can feel somewhat different atmosphere at the different areas of the booths if a joint fair is held. Hunters are ready to go a long way (to the fairs) to be together - while fishing fairs are much more often brought closer georaphically to the anglers - sometimes they are cloned and/or re-branded.. Did you see the latest ...

We love and respect sports - even if my grandson said: I prefer watch soccer on TV, it is not as tiresome as playing it.. Fishing and Hunting Trade Fairs are becoming very popular. One may say, because we love to be outdoors. Or we can say that Fishing & Hunting are great sports - but ask the rabbit!. F&H trade fairs are often held together and though being patient is common requirement, yet I noticed major differences among the typical visitors. Anglers are more reserved - fishing is a lone fight - just me and the fish - while hunting needs an accord of team. A joint, common experience for hunters - at fairs they are jolly, loud, noisy - and happy together.. Therefore one can feel somewhat different atmosphere at the different areas of the booths if a joint fair is held. Hunters are ready to go a long way (to the fairs) to be together - while fishing fairs are much more often brought closer georaphically to the anglers - sometimes they are cloned and/or re-branded.. Did you see the latest ...

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Researchers at the University of Pennsylvania School of Medicine have found that juvenile cells on their way to becoming mature immune cells can develop into either T cells or other blood-cell types versus only being committed to the T-cell path. The findings appear in this weeks issue of Nature, and have implications for better understanding how T-cell leukemias and other disorders arise.

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