Amicetin, an antibacterial and antiviral agent, belongs to a group of disaccharide nucleoside antibiotics featuring an alpha-(1 -> 4)-glycoside bond in the disaccharide moiety. In this study, the amicetin biosynthesis gene cluster was cloned from Streptomyces vinaceusdrappus NRRL 2363 and localized on a 37-kb contiguous DNA region. Heterologous expression of the amicetin biosynthesis gene cluster in Streptomyces lividans TK64 resulted in the production of amicetin and its analogues, thereby confirming the identity of the ami gene cluster. In silico sequence analysis revealed that 21 genes were putatively involved in amicetin biosynthesis, including 3 for regulation and transportation, 10 for disaccharide biosynthesis, and 8 for the formation of the amicetin skeleton by the linkage of cytosine, p-aminobenzoic acid (PABA), and the terminal (+)-alpha-methylserine moieties. The inactivation of the benzoate coenzyme A (benzoate-CoA) ligase gene amiL and the N-acetyltransferase gene amiF led to two ...
In the search for effective, selective, and nontoxic antiviral and antitumor agents, a variety of strategies have been devised to design nucleoside analogues. Here we have described the versatile synthesis of beta-L-1,3- thiazolidine nucleoside analogues. These analogues are all derived from the key stereochemically defined intermediate Ntert- butoxy-carbonyl-4-hydroxymethyl- 1,3-thiazolidine-2-ol which was accessible in 57% yield starting from L-Cysteine methylester hydrochloride. N-tert-butoxycarbonyl-2-acyloxy-4-trityloxymethyl-1,3-thiazolidine was coupled with the pyrimidine bases in the presence of Lewis acids stannic chloride or trimethyl silyl triflate following Vorbruggen procedure. Proof of the structure and configuration was obtained through 1H NMR, 13C NMR, Mass, elemental analysis and NOE experiments. Docking and antitumor activity of these nucleoside analogues are also reported.. ...
9979, Phase 1 and Pharmacology Study of Oral 5-iodo-2-pyrimidinone-2-deoxyribose (IPdR) as a Prodrug for IUdR-Mediated Tumor Radiosensitization in Brain ...
TY - JOUR. T1 - Biochemical properties of 5 sulfur substituted pyrimidine nucleosides and nucleotides. AU - Bardos, T. J.. AU - Aradi, J.. AU - Ho, Y. K.. AU - Kalman, T. I.. PY - 1975. Y1 - 1975. UR - http://www.scopus.com/inward/record.url?scp=0016590249&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0016590249&partnerID=8YFLogxK. M3 - Article. C2 - 1059371. AN - SCOPUS:0016590249. VL - Vol.255. SP - 522. EP - 531. JO - Annals of the New York Academy of Sciences. JF - Annals of the New York Academy of Sciences. SN - 0077-8923. ER - ...
References for Abcams Anti-Pseudomonas aeruginosa Group Ds antibody (ab78754). Please let us know if you have used this product in your publication
Background Sansanmycins are uridyl peptide antibiotics (UPAs), that are inhibitors of translocase We (MraY) and stop the bacterial cell wall structure biosynthesis. sansanmycin analogues, MX-1 to MX-10, had been purified and discovered by electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic buy Rosiglitazone (BRL-49653) resonance (NMR). The bioassay of the sansanmycin analogues demonstrated that sansanmycin MX-1, MX-2, MX-4, MX-7 and MX-6 exhibited equivalent strength to sansanmycin A against H37Rv, aswell as multi-drug-resistant (MDR) and extensive-drug-resistant (XDR) strains. Furthermore, sansanmycin MX-4 and MX-2 displayed far better balance than sansanmycin A. Conclusions We showed that SsaX is in charge of the biosynthesis of deletion mutant SS/XKO and ten of these had been purified and structurally discovered. Among them, MX-4 and MX-2 showed promising anti-MDR and anti-XDR tuberculosis activity and better balance than sansanmycin A. These outcomes indicated that ...
(3-Thien-2-ylphenyl)methanol, ≥97%, Maybridge 5g (3-Thien-2-ylphenyl)methanol, ≥97%, Maybridge Tetraphenylc to Thiopheneb -Organics
Benzo(b)thien-3-yl morpholinomethyl ketone hydrochloride | C14H16ClNO2S | CID 23798 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
(6-Thien-2-ylimidazo[2,1-b][1,3]thiazol-3-yl)acetic acid/ACM878259871 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
Learn more about 1-thien-2-ylcarbonyl-piperidin-3-amine-hydrochloride. We enable science by offering product choice, services, process excellence and our people make it happen.
This compound belongs to the class of organic compounds known as 3-thia pyrimidine nucleosides. These are nucleoside analogues with a structure that consists of a pyrimidine base, which is N-substituted at the 1-position with a 3-thia derivative (1,3-oxazolidine) of the ribose moiety that is characteristic of nucleosides. ...
A stereoselective radical-mediated hydrogermylation of the protected 5-ethynyluracil nucleosides with trialkyl-, triaryl,- or tris(trimethylsilyl)germanes gave (Z)-5-(2-germylvinyl)uridine, 2-deoxyuridine, or ara-uridine as major products. Reaction of the β-triphenylgermyl vinyl radical intermediate with oxygen and fragmentation of the resulting peroxyradical provided also 5-[2-(triphenylgermyl)acetyl]pyrimidine nucleosides in low to moderate yields. Thermal isomerization of the ...
A method of making a radiolabeled pyrimidine nucleoside or nucleotide is described. In the method, a stannylated pyrimidine nucleoside or nucleotide is contacted in an aqueous solution with a radioactive iodide, bromide, chlorine or astatine ion in the presence of an acidic hydrogen peroxide oxidizing solution comprising at least a 3:1 ratio of 30% hydrogen peroxide to 1N acid (v/v), whereby a water soluble pyrimidine nucleoside or nucleotide labeled with radioactive iodine, bromine, chlorine or astatine is formed. Kits suitable for practicing the method are also disclosed.
Anti-Pseudomonas aeruginosa Serotype 2B Antibody, clone 1253/17 , Mouse Anti-Bacterial Monoclonal Antibody validated in E (ABD10730), Abgent
Synthesis of Purine Acyclonucleosides via Ribofuranose Ring Cleavage of Purine Nucleosides by Diisobutylaluminum Hydride|span||/span| | Kosaku Hirota; Yasunari Monguchi; Hironao Sajiki | download | BookSC. Download books for free. Find books
N-Methyl-[1-(thien-2-ylmethyl)piperid-4-yl]methylamine, 97%, Maybridge Amber Glass Bottle; 5g N-Methyl-[1-(thien-2-ylmethyl)piperid-4-yl]methylamine, 97%, Maybridge...
1b) 2 5-dehydroinosine + O2 = 2 9-riburonosylhypoxanthine. Systematic name: nucleoside:oxygen 5-oxidoreductase. Comments: Other purine and pyrimidine nucleosides (as well as 2-deoxyribonucleosides) are substrates, but ribose and nucleotides are not substrates. The overall reaction takes place in two separate steps, with the 5-dehydro nucleoside being released from the enzyme to serve as substrate for the second reaction. This enzyme differs from EC 1.1.3.39, nucleoside oxidase (H2O2-forming), as it produces water rather than hydrogen peroxide.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: References:. 1. Isono, Y., Sudo, T. and Hoshino, M. Purification and reaction of a new enzyme, nucleoside oxidase. Agric. Biol. Chem. 53 (1989) 1663-1669.. 2. Isono, Y., Sudo, T. and Hoshino, M. Properties of a new enzyme, nucleoside oxidase, from Pseudomonas maltophilia LB-86. Agric. Biol. Chem. 53 (1989) 1671-1677.. ...
Unlike herpes viruses, human immunodeficiency virus and other retroviruses do not encode specific enzymes required for the metabolism of the purine or pyrimidine nucleosides to their corresponding...
The enzyme from Escherichia coli catalyzes the 2-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNA(Leu)CmAA and tRNA(Leu)cmnm(5)UmAA. -!- The enzyme is selective for the two tRNA(Leu) isoacceptors and only methylates these when they present the correct anticodon loop sequence and modification pattern. -!- Specifically, YibK requires a pyrimidine nucleoside at position 34, it has a clear preference for an adenosine at position 35, and it fails to methylate without prior addition of the N(6)-(isopentenyl)- 2-methylthioadenosine modification at position 37 ...
Boosting up performance of inverted photovoltaic cells from bis(alkyl- thien-2-yl)dithieno[2,3-d:2′,3′-d′]benzo[1,2-b:4′,5′-b′]dithiophene-based copolymers by advantageous vertical phase separation ...
Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA(Ile)-lysidine synthetase, C-terminal domain (TIGR02433; EC 6.-.-.-; HMM-score: 71.6) ...
Learn more about 4-Amino-5-iodo-2-methoxybenzenecarboxylic acid. We enable science by offering product choice, services, process excellence and our people make it happen.
The pyrimidine nucleoside analog FIAC and its primary deaminated uracil metabolite FIAU are highly and specifically active compounds in vitro against several herpes group viruses, particularly herpes simplex virus (HSV) types 1 and 2, varicella zoster (VZV), and cytomegalovirus (CMV), as well as hepatitis B virus (HBV). Since FIAU is the primary metabolite of FIAC and the administration of FIAU simplifies the metabolism of FIAC, it is anticipated from clinical studies of FIAC that FIAU will be tolerated at least as well as FIAC. A single-dose, pharmacokinetic (blood level) study showed that FIAC, when taken orally, is readily absorbed into the bloodstream, and most of it is converted to FIAU. Daily oral doses are expected to provide concentrations of FIAU exceeding the in vitro minimum inhibitory concentration for nearly all the herpes group viruses.. HIV-infected patients with Karnofsky scores at least 80 (with or without documented recurrent herpes group infections) are successively entered ...
The pyrimidine nucleoside analog FIAC and its primary deaminated uracil metabolite FIAU are highly and specifically active compounds in vitro against several herpes group viruses, particularly herpes simplex virus (HSV) types 1 and 2, varicella zoster (VZV), and cytomegalovirus (CMV), as well as hepatitis B virus (HBV). Since FIAU is the primary metabolite of FIAC and the administration of FIAU simplifies the metabolism of FIAC, it is anticipated from clinical studies of FIAC that FIAU will be tolerated at least as well as FIAC. A single-dose, pharmacokinetic (blood level) study showed that FIAC, when taken orally, is readily absorbed into the bloodstream, and most of it is converted to FIAU. Daily oral doses are expected to provide concentrations of FIAU exceeding the in vitro minimum inhibitory concentration for nearly all the herpes group viruses.. HIV-infected patients with Karnofsky scores at least 80 (with or without documented recurrent herpes group infections) are successively entered ...
FOSTER CITY, Calif., Jun 24, 2003 (BUSINESS WIRE) -- Gilead Sciences, Inc. (Nasdaq: GILD) today announced that the company is ending its licensing agreement with Bukwang Pharm. Ind. Co., Ltd for the development and commercialization of clevudine. Formerly known as L-FMAU, clevudine is an investigational pyrimidine nucleoside analogue currently in Phase I/II development for the treatment of chronic hepatitis B. The termination will be effective August 6, 2003. In February 1998, Triangle Pharmaceuticals entered into a licensing agreement with Bukwang for worldwide rights to clevudine in all markets except Korea. In January 2003, Gilead acquired Triangle Pharmaceuticals. Under the terms of the licensing agreement, Gilead does not owe any further payments to Bukwang. Gilead will meet its ongoing obligations under the agreement until such time that they can be transferred to Bukwang or a new partner. "We have valued our partnership with Bukwang, and while clevudine is an interesting compound, we have ...
Mouse monoclonal Pseudomonas aeruginosa serotype 9 antibody [95/159] validated for ELISA, ICC/IF. Immunogen corresponding to tissue, cells or virus
Chemical Synthesis of Nucleoside Analogues covers all the major classes of nucleosides, including pronucleotides, C-nucleosides, carbanucleosides, and PNA monomers which have shown great promise as starting points for the synthesis of nucleoside analogues. The book also includes experimental procedures for key reactions related to the synthesis of nucleoside analogues, providing a valuable tool for the preparation of a number of different compounds.. Throughout the book, chemical schemes and figures help readers better understand the chemical structures of nucleoside analogues and the methods used to synthesize them. Extensive references serve as a gateway to the growing body of original research studies and reviews in the field.. Synthetically modified nucleosides have proven their value as therapeutic drugs, in particular as antiviral and antitumor agents. However, many of these nucleoside analogues have undesirable side effects. With Chemical Synthesis of Nucleoside Analogues as their guide, ...
The AUA codon-specific isoleucine tRNA (tRNA(Ile)) in eubacteria has the posttranscriptionally modified nucleoside lysidine (L) at the wobble position of the anticodon (position 34). This modification is a lysine-containing cytidine derivative that converts both the codon specificity of tRNA(Ile) from AUG to AUA and its amino acid specificity from methionine to isoleucine. We identified an essential gene (tilS; tRNA(Ile)-lysidine synthetase) that is responsible for lysidine formation in both Bacillus subtilis and Escherichia coli. The recombinant enzyme complexed specifically with tRNA(Ile) and synthesized L by utilizing ATP and lysine as substrates. The lysidine synthesis of this enzyme was shown to directly convert the amino acid specificity of tRNA(Ile) from methionine to isoleucine in vitro. Partial inactivation of tilS in vivo resulted in an AUA codon-dependent translational defect, which supports the notion that TilS is an RNA-modifying enzyme that plays a critical role in the accurate ...
The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis.
The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis.
Other purine and pyrimidine nucleosides (as well as 2-deoxyribonucleosides) are substrates, but ribose and nucleotides are not substrates. The overall reaction takes pla
2001: Recent molecular advances in studies of the concentrative Na+-dependent nucleoside transporter (CNT) family: identification and characterization of novel human and mouse proteins (hCNT3 and mCNT3) broadly selective for purine and pyrimidine nucleosides (system cib ...
Literature References: Fluorinated pyrimidine nucleoside with cytostatic activity. Prepn: A. F. Cook, US 4071680 (1978 to Hoffmann-La Roche); H. Hrebabecky, J. Beranek, Nucleic Acids Res. 5, 1029 (1978); A. F. Cook et al., J. Med. Chem. 22, 1330 (1979). Stereospecific synthesis: J. Kiss et al., Helv. Chim. Acta 65, 1522 (1982). Mechanism of action studies: H.-R. Hartmann, A. Matter, Cancer Res. 42, 2412 (1982); R. D. Armstrong et al., Cancer Chemother. Pharmacol. 11, 102 (1983). Kinetics and metabolism in humans: J.-P. Sommadossi et al., Cancer Res. 43, 930 (1983). Clinical trials in colorectal carcinoma: R. Abele et al., J. Clin. Oncol. 1, 750 (1983); S. D. Fossa et al., Cancer Chemother. Pharmacol. 15, 161 (1985). Series of articles on animal toxicology: Yakuri to Chiryo 13, Suppl. 2, 221-430 (1985); acute toxicity: M. Shimizu et al., ibid. 209, C.A. 104, 14673z-14678e (1986). Evaluation of neurotoxicity in humans: M. S. Heier, S. D. Fossa, Acta Neurol. Scand. 73, 449 (1986). ...
I should think the same principle would apply with nucleoside analogs. They apply the treatment only to the tumor cells, selectively stopping them from dividing by disrupting DNA replication only in those cells. But of course the big problem with curing cancer has always been that any treatment for cancer will cause damage to the patients normal cells as well, which is why so much cancer research is focused on trying to find differences between cancer cells and normal cells that might be exploited to develop more precisely targeted therapies ...
Nucleosides are the building blocks for life, they are found in everything from DNA to RNA. This one-step reference is the first comprehensive resource...
nucleoside: Any of various compounds consisting of a sugar, usually ribose or deoxyribose, and a purine or pyrimidine base, especially a compound obtained by hydrolysis of a nucleic acid, such as adenosine or guanine.
MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisadenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 76) ...
Pseudomonas aeruginosa Serotype 2B antibody LS-C538938 is an FITC-conjugated mouse monoclonal antibody to pseudomonas aeruginosa Pseudomonas aeruginosa Serotype 2B. Validated for ELISA.
Objective: Thymidine phosphorylase (TP) is a key enzyme in the pyrimidine nucleoside salvage pathway and its expression is upregulated in a wide variety of solid tumors. In mice, we previously observed high and specific accumulation levels of our TP imaging probe, radioiodinated 5-iodo-6-[(2-iminoimidazolidinyl)methyl]uracil (IIMU) not only in high-TP-expressing tumors, but also in the liver and small intestine. To clarify the reason for the high accumulation levels of radioiodinated IIMU in the liver and small intestine, we investigated the expression levels of TP in mice in comparison with the biodistribution of radioiodinated IIMU (123I-IIMU). Methods: BALB/cCrSlc mice were injected with 123I-IIMU, and the radioactivity levels [%ID/g (normalized to a mouse of 25 g body weight)] in the tissues of interest were determined 0.5, 1, 3 and 24 h after the injection (n = 5, each time point). To determine the expression levels of TP, BALB/cCrSlc and ddy mice (n = 3/each strain) were euthanized, and ...
These two volumes record the scientific and clinical work presented at the VIIth International and 3rd European joint symposium on purine and pyrimidine metabolism in man held at the Bournemouth Inter
The aim of this thesis was to determine the role of nucleoside analog activating and deactivating enzymes in nucleoside analog metabolism and resistance development. Nucleoside analogs are anti-cancer drogs and are often used to treat different leukemias, attributably to presence of high levels of nucleoside analog activating enzymes in hematopoietic cells. More recently some of the newer analogs have been used successfully to treat solid tumors as well.. We have used human leukemic cell lines, and isolated cells from patients with leukemia, to investigate the nucleoside analog activating enzymes deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK) and some of the deactivating enzymes called 5nucleotidases (5-NTs). We have measured mRNA expressions and enzymatic activities and correlated them with the cytotoxic response to nuc1eoside analogs and changes in cell cycle progression. We optimized and evaluated a siRNA-transfection method and decreased the activities of dCK and dGK in two ...
Pyrimidine-5-Nucleotidase Deficiency (Pyrimidine 5-Nucleotidase Deficiency): Read more about Symptoms, Diagnosis, Treatment, Complications, Causes and Prognosis.
An imidazole antifungal agent used topically as a skin cream or in the form of vaginal ovules. Since the last update date we have not found published data on its excretion in breastmilk. The mean plasma concentration after the application of 300 mg of vaginal sertaconazole was 1.79 ± 0.70 micrograms/L (Wang 2009). Its insignificant or zero systemic absorption through vaginal mucosa or skin (Croxtall 2009, Wang 2009, Susilo 2006, Farré 1992) and its high plasma protein binding makes transfer to milk in significant quantities unlikely. You should wash your hands well after application. Do not apply on breast-nipple-areola or clean before breastfeeding.
Pearson D, Hienzsch A, Wagner M, Globisch D, Reiter V, Özden D, Carell T Chem. Commun. 47 (18) 5196 [2011-00-00; online 2011-00-00] RNA nucleosides are often naturally modified into complex non-canonical structures with key biological functions. Here we report LC-MS quantification of the Ar(p) and Gr(p) 2-ribosylated nucleosides in tRNA using deuterium labelled standards, and the first detection of Gr(p) in complex fungi. Fellow QC bibliography QC xrefs PubMed 21448475. DOI 10.1039/c1cc11011j. Crossref 10.1039/c1cc11011j. ...
Using a new process, scientists can create new nucleoside analogues months earlier than with the previous method, paving the way for quicker drug discoveries.
PIPERAZIN, a substance formed by the action of sodium glycol on ethylene-diamine hydrochloride, consisting of small alkaline deliquescent crystals with a saline taste and soluble in water. It was originally introduced into medicine as a solvent for uric acid. When taken into the body the drug is partly oxidized and partly eliminated unchanged. Outside the body piperazin has a remarkable power of dissolving uric acid and producing a soluble urate, but in clinical experience it has not proved equally successful. Lycetol, lysidine and sidonal are bodies having similar action.. ...
The oxygen doubled bonded to the C-2 invades the space of the hydrogen at C-2 and to a lesser extent the oxygen of the furanose ring. Since both pyrimidines found in DNA have an oxygen at the C-2 position, nucleosides and nucleotides of these pyrimidines only adopt the anti conformation and they do so even in Z-DNA. ...
Nascent Pharmaceuticals are developing a nucleoside prodrug for the treatment of cancer. The prodrug is activated into a locally cytotoxic agent by high levels
The pyrimidine salvage enzyme, nucleoside hydrolase, is catalyzes the irreversible hydrolysis of nucleosides into the free nucleic acid base and D-ribose. Nucleoside hydrolases have varying degrees of specificity towards purine and pyrimidine nucleosides. In E. coli, three genes were found that encode homologues of several known nucleoside hydrolases in protozoa. All three genes (designated yaaF, yeiK, and ybeK) were amplified by PCR and cloned. Two of the gene products (yeiK and ybeK) encode pyrimidine-specific nucleoside hydrolases, while the third (yaaF) encodes a nonspecific nucleoside hydrolase. All three were expressed at low levels and had different modes of regulation. As a comparative analysis, the homologous genes of Pseudomonas aeruginosa and P. fluorescens (designated nuh) were cloned. Both were determined to encode nonspecific nucleoside hydrolases. The nucleoside hydrolases of the pseudomonads exhibited markedly different modes of regulation. Both have unique promoter structures and
Literature References: First reported nucleoside antibiotic. Isoln from culture fluids of Cordyceps militaris (Linn.) Link: K. G. Cunningham et al., J. Chem. Soc. 1951, 2299; N. M. Kredich, A. J. Guarino, Biochim. Biophys. Acta 41, 363 (1960). Proposed structure: H. R. Bentley et al., J. Chem. Soc. 1951, 2301. Identity with 3¢-deoxyadenosine and revised structure: E. A. Kaczka et al., Biochem. Biophys. Res. Commun. 14, 456 (1964). Biosynthesis: R. Suhadolnik et al., J. Am. Chem. Soc. 86, 948 (1964). Synthesis: A. R. Todd, T. L. Ulbricht, J. Chem. Soc. 1960, 3275; W. W. Lee et al., J. Am. Chem. Soc. 83, 1906 (1961); E. Walton et al., ibid. 86, 2952 (1964); Y. Ito et al., ibid. 103, 6739 (1981). Cordycepin and cordycepin triphosphate have been used extensively in the study of messenger RNA transcription, see H. T. Shigeura, G. E. Boxer, Biochem. Biophys. Res. Commun. 17, 758 (1964); S. Penman et al., Proc. Natl. Acad. Sci. USA 67, 1878 (1970). Reviews: J. J. Fox. et al., "Nucleoside Antibiotics" ...