Pseudomonas chlororaphis is a bacterium used as a soil inoculant in agriculture and horticulture. It can act as a biocontrol agent against certain fungal plant pathogens via production of phenazine-type antibiotics. Based on 16S rRNA analysis, similar species have been placed in its group. P. chlororaphis lends its name to a subgroup within the genus Pseudomonas. The other members of the P. chlororaphis subgroup are P. aurantiaca, P. aureofaciens, P. fragi, P. lundensis, and P. taetrolens. Chin-A-Woeng TF, et al. (2000). Root colonization by phenazine-1-carboxamide-producing bacterium Pseudomonas chlororaphis PCL1391 is essential for biocontrol of tomato foot and root rot. Mol Plant Microbe Interact. 13 (12): 1340-5. doi:10.1094/MPMI.2000.13.12.1340. PMID 11106026. Anzai; Kim, H; Park, JY; Wakabayashi, H; Oyaizu, H; et al. (Jul 2000). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol. 50 (4): 1563-89. doi:10.1099/00207713-50-4-1563. PMID ...

Penyalver, R., Bertolini, E., Olmos, A., Garcia, A., Cambra, M., Lopez, M.M. (2001). Detection of Pseudomonas savastanoi pv. savastanoi (Pss), on asymptomatic olive plant tissues by enrichment-PCR. , , 424 ...

Pseudomonas species are opportunistic pathogens with implications in a wide range of diseases including cystic fibrosis and sickle cell anaemia. Because of their status as multidrug resistant (MDR) and extremely drug resistant (XDR) bacteria Pseudomonas species represent a threat to public health. Prevalence, antibiogram and associated antibiotic resistant genes of Pseudomonas species isolated from freshwater and mixed liquor environments in the Eastern Cape Province of South Africa were assessed. Polymerase chain reaction (PCR) based technique was used to identify the isolates and screen for antibiotic resistant genes. The result shows occurrence of Pseudomonas spp. in freshwater and mixed liquor as follows: 71.42% and 37.5% (P. putida), 14.28% and 31.25% (P. flourescens), 7.14% and 6.25% (P. aeruginosa) and 7.14% and 25% for other Pseudomonas species respectively. Disk diffusion antibiogram of the Pseudomonas isolates from the two locations showed 100% resistance to penicillin, oxacillin, clindamycin,

This study analysed the phenotypic and genotypic variation among 511 Pseudomonas savastanoi pv. phaseolicola (Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean (Vigna radiata) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phenotypically screened. Our in planta screening against a set of four mungbean cultivars with known susceptible and resistant reactions revealed five distinctive pathotypes. Isolates belonging to pathotype 2 were the most prevalent at 84% and were found to be highly pathogenic towards all tested mungbean genotypes. Genomic variation was investigated for 205 isolates using DNA fingerprints, splitting the halo blight pathogen population into two broad genetic lineages. Further genetic testing for two known avirulence genes, avrPphE and avrPphF, identified the avrPphE gene in all the tested isolates and avrPphF present in all but two. To identify candidate avirulence genes unique to Psp isolates infecting ...

Plant Disease 97:1381.1-1381.1...Plant Disease 97:1381.1-1381.1...First Report of Tomato Pith Necrosis (Pseudomonas corrugata) on Tomato (Solanum lycopersicum) in Washington...M. Powell , B. Gundersen , and C. A. Miles , Departments of Plant Pathology and Horticulture, Washington State University Mount Vernon NWREC, 16650 State Route 536, Mount Vernon 98273 ; J. L. Humann and B. K. Schroeder , Department of Plant Pathology, Washi...

Looking for online definition of Pseudomonas facilis in the Medical Dictionary? Pseudomonas facilis explanation free. What is Pseudomonas facilis? Meaning of Pseudomonas facilis medical term. What does Pseudomonas facilis mean?

TY - JOUR. T1 - Isolation and structural elucidation of syringostatins, phytotoxins produced by pseudomonas syringae pv. syringae lilac isolate. AU - Fukuchi, Naoyuki. AU - Isogai, Akira. AU - Nakayama, Jiro. AU - Takayama, Seiji. AU - Yamashita, Shuichi. AU - Suyama, Kazuo. AU - Suzuki, Akinori. PY - 1992. Y1 - 1992. N2 - A bacterial strain of Pseudomonas syringae pv. syringae isolated from lilac was found to produce a homologous mixture of phytotoxins different from syringomycin and syringotoxin. The toxins were termed syringostatins and the structures of the main components, syringostatins A and B, were determined by 2D-NMR spectroscopy and mass spectrometry. Minor component structures were elucidated from mass/mass spectra.. AB - A bacterial strain of Pseudomonas syringae pv. syringae isolated from lilac was found to produce a homologous mixture of phytotoxins different from syringomycin and syringotoxin. The toxins were termed syringostatins and the structures of the main components, ...

TY - JOUR. T1 - Analysis of the role of the Pseudomonas syringae pv. syringae HrpZ harpin in elicitation of the hypersensitive response in tobacco using functionally non-polar hrpZ deletion mutations, truncated HrpZ fragments, and hrmA mutations. AU - Alfano, James R.. AU - Bauer, David W.. AU - Milos, Timothy M.. AU - Collmer, Alan. PY - 1996. Y1 - 1996. N2 - Pseudomonas syringae pv. syringae, like many plant pathogenic bacteria, secretes a harpin protein that can elicit the hypersensitive response (HR), a defensive cellular suicide, in non-host plants. The harpin-encoding hrpZ gene is located in an operon that also encodes Hrp secretion pathway components and is part of the functional cluster of hrp genes carried on cosmid pHIR11 that enables saprophytic bacteria like Escherichia coli and Pseudomonas fluorescens to elicit the HR in tobacco leaves. We have constructed functionally non-polar hrpZ deletion mutations, revealing that HrpZ is necessary for saprophytic bacteria carrying pHIR11 to ...

Pseudomonas syringae pathovar phaseolicola ATCC ® BAA-978D™ Designation: Genomic DNA from Pseudomonas syringae pathovar phaseolicola strain 1448A TypeStrain=False Application:

Pseudomonas mosselii is a Gram-negative, rod-shaped, bacterium clinically isolated in France. Based on 16S rRNA analysis, P. mosselii has been placed in the P. putida group. Dabboussi; Hamze, M; Singer, E; Geoffroy, V; Meyer, JM; Izard, D; et al. (Mar 2002). Pseudomonas mosselii sp. nov., a novel species isolated from clinical specimens. Int J Syst Evol Microbiol. 52 (Pt 2): 363-76. doi:10.1099/00207713-52-2-363. PMID 11931144. Anzai; Kim, H; Park, JY; Wakabayashi, H; Oyaizu, H; et al. (Jul 2000). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol. 50 (4): 1563-89. doi:10.1099/00207713-50-4-1563. PMID 10939664. Type strain of Pseudomonas mosselii at BacDive - the Bacterial Diversity ...

Pseudomonas syringae is pathogenic in a wide variety of plants, causing diseases with economic impacts. Pseudomonas syringae pathovars produce several toxins that can function as virulence factors and contribute to disease symptoms. These virulence factors include antimetabolite toxins, such as tabtoxin, phaseolotoxin and mangotoxin, which target enzymes in the pathways of amino acid metabolism. The antimetabolite toxins are generally located in gene clusters present in the flexible genomes of specific strains. These gene clusters are typically present in blocks of genes that appear to be integrated into specific sites in the P. syringae core genome. A general overview of the genetic organization and biosynthetic and regulatory functions of these genetic traits of the antimetabolite toxins will be given in the present work.

TY - JOUR. T1 - The hrpK operon of Pseudomonas syringae pv. tomato DC3000 encodes two proteins secreted by the type III (Hrp) protein secretion system. T2 - HopB1 and HrpK, a putative type III translocator. AU - Petnicki-Ocwieja, Tanja. AU - Van Dijk, Karin. AU - Alfano, James R.. PY - 2005/1. Y1 - 2005/1. N2 - Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant cells for pathogenicity. The P. syringae TTSS is encoded by hrp-hrc genes that reside in a central region of a pathogenicity island (Pai). Flanking one side of this Pai is the exchangeable effector locus (EEL). We characterized the transcriptional expression of the open reading frames (ORFs) within the EEL of P. syringae pv. tomato DC3000. One of these ORFs, PSPTO1406 (hopB1) is expressed in the same transcriptional unit as hrpK. Both HopB1 and HrpK were secreted in culture and translocated into plant cells ...

Pseudomonas syringae pv. savastanoi y P. syringae pv. phaseolicola son dos patógenos de plantas, incluidos en los 60 patovares del grupo P. syringae. Las dos bacterias causan enfermedad en diferentes huéspedes y con síntomas muy distintos, la primera tumores en olivo y la segunda lesiones en judía, y son organismos modelos de estudio en la identificación de los determinantes que definen el espectro de huésped. La definición del espectro de huésped puede estar determinada por la acción conjunta de proteínas llamadas efectores, que son secretadas por un sistema de secreción tipo III a la célula vegetal, dando lugar a la inhibición de las respuestas de defensa de la planta. El objetivo del trabajo ha sido la identificación de genes de efectores de P. syringae pv. savastanoi que induzcan respuesta de incompatibilidad en judía, para lo que se abordó la clonación y ensayo de 11 efectores. De éstos, sólo se pudieron obtener clones de los efectores AER-0000629 y AER-0001936, que se ...

Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics and proteomics studies with other P. putida strains, the P. putida KT2440 genomic database serves as standard reference. The utility of KT2440 whole-genome, high-density oligonucleotide microarrays for transcriptomics studies of other Pseudomonas strains was investigated. To this end, microarray hybridizations were performed with genomic DNAs of subcultures of P. putida KT2440 (DSM6125), the type strain (DSM291T), plasmid pWW0-containing KT2440-derivative strain mt-2 (DSM3931), the solvent-tolerant P. putida S12, and several other Pseudomonas strains. Depending on the strain tested, 22 to 99% of all genetic elements were identified in the genomic DNAs. The efficacy of these microarrays to study cellular function was determined for all strains included in the study. The vast majority of DSM6125 genes encoding proteins of primary metabolism and genes involved in the catabolism of aromatic compounds ...

Carbapenems are critically important antimicrobials as a last line of defense against multidrug-resistant Gram-negative bacterial infections (1, 2). As such, the increasing prevalence of carbapenemase-producing isolates in animal husbandry is of great concern. While the metallo-β-lactamase (MBL)-producing bacteria have been commonly identified from food animals (3-7), blaMBL-carrying Pseudomonas spp. are rarely reported in animal husbandry or the surrounding environment. Although we have reported the high prevalence of NDM in Enterobacteriaceae isolates from poultry production in Shandong Province (7), carbapenemase-producing non-Enterobacteriaceae isolates have not been identified in the same region. Here, we report four chromosome-borne VIM-positive Pseudomonas isolates: one Pseudomonas aeruginosa isolate from a swallow (Yanornis martini), one Pseudomonas putida isolate from a fly, and two P. putida isolates from chickens. The blaVIM-2 gene was identified in the P. aeruginosa isolate, but 27 ...

Synonyms for blepharitis marginalis in Free Thesaurus. Antonyms for blepharitis marginalis. 3 words related to blepharitis: inflammation, redness, rubor. What are synonyms for blepharitis marginalis?

TY - JOUR. T1 - Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate. T2 - Insight into the basis of its substrate specificity. AU - Kim, Youngsoo. AU - Hol, Wim G.J.. N1 - Funding Information: We thank Ethan Merritt, Stephen Suresh and Craig Behnke for their helpful discussions, Jungwoo Choe, Stewart Turley and Mic Feese for collecting data, and the SBC-CAT staff, in particular Randy Alkire, Stephan Ginell and Rongguang Zhang for their technical assistance on the APS SBC-CAT beamline. Use of the Argonne National Laboratory Structural Biology Center beamlines at the APS was supported by the US Department of Energy Office of Energy Research, under contract No. W-31-109-ENG-38. We also thank Francis Athappilly, Irwin Hirsh, and Claudia Roach of the Biomolecular Structure Center for maintaining our computer facilities and helping with the protein expression, purification and crystallization. W.G.J.H. acknowledges a major equipment grant from the ...

Fruits is a scientific journal for original articles and reviews on fruit crops in temperate, Mediterranean, subtropical and tropical regions

We performed a numerical analysis of the results of biochemical and nutritional tests done with strains of nonfluorescent plant-pathogenic Pseudomonas spp. A total of 57 tests were used, and determinative tests which discriminated between taxa were identified. Pseudomonas andropogonis, P. caryophylli, P. corrugata, P. glumae, P. plantarii, and P. rubrisubalbicans were distinguished as distinct members of the genus Pseudomonas. Strains of P. ficuserectae and P. meliae formed a single cluster having affinities with P. syringae. Strains of P. cissicola were allocated to Agrobacterium spp. Strains of P. avenae, P. cattleyae, P. pseudoalcaligenes subsp. citrulli, P. pseudoalcaligenes subsp. konjaci, and P. rubrilineans formed a single relatively homogeneous cluster, within which three subclusters were discerned. Strains presently identified as P. avenae and as P. rubrilineans, including the type strain of P. cattleyae, formed a single homogeneous subcluster. Strains of P. pseudoalcaligenes subsp. citrulli

Tytuł projektu: Udostępnianie cyfrowe zasobów polskich czasopism z nauk przyrodniczych i rolniczych w bazie AGRO. Nr umowy: POPC.02.03.01-00-0038/18-00 (okres realizacji 2018-2021). Kwota dofinansowania: 7 442 980,00 z. W ramach Programu Operacyjnego Polska Cyfrowa na lata 2014-2020, Oś Priorytetowa nr 2 E-administracja i otwarty rząd Działanie nr 2.3 Cyfrowa dostępność i użyteczność informacji sektora publicznego Poddziałanie nr 2.3.1 Cyfrowe udostępnienie informacji sektora publicznego ze źródeł administracyjnych i zasobów nauki (typ projektu: cyfrowe udostępnienie zasobów nauki) Instytucja Finansująca: Centrum Projektów Polska Cyfrowa ...

Competition among indigenous and inoculated 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was studied in a native Kansas prairie soil following 2,4-D additions. The soil was inoculated with four different 2,4-D-degrading strains at densities of 10(3) cells per g of soil; the organisms used were Pseudomonas cepacia DBO1(pJP4) and three Michigan soil isolates, strain 745, Sphingomonas paucimobilis 1443, and Pseudomonas pickettii 712. Following 2,4-D additions, total soil DNA was extracted and analyzed on Southern blots by using a tfdA gene probe which detected three of the strains and another probe that detected the fourth strain, S. paucimobilis 1443, which belongs to a different class of 2,4-D degraders. P. cepacia DBO1(pJP4), a constructed strain, outcompeted the other added strains and the indigenous 2,4-D-degrading populations. The S. paucimobilis population was the secondary dominant population, and strain 745 and P. pickettii were not detected. Relative fitness coefficients determined

Bacterial stem blight of alfalfa occurs sporadically in the central and western U.S. Yield losses of up to 50% of the first harvest can occur with some cultivars. Developing resistant cultivars is hampered by lack of information on the pathogen and a standard test for evaluating plant germplasm. Bacteria producing a fluorescent pigment were isolated on Kings B agar from alfalfa with symptoms of bacterial stem blight from near Cheyenne, WY. The strain ALF3 was tentatively identified as Pseudomonas syringae pv. syringae based on 16S rDNA sequence and PCR amplification of syrB for lipodepsinonapeptide toxin production. Multilocus sequence analysis indicated that ALF3 falls within a clade containing strains of P. syringae pv. syringae with closest affinity to FF5 from pear. Comparison of a draft whole-genome sequence of ALF3 further confirmed that ALF3 most closely resembles FF5 (~96% sequence identity) and P. syringae pv. aptata DSM50252 from beet. Approximately 60 genes were unique to ALF3, ...

TY - JOUR. T1 - Immunological detection of syringopeptins produced by Pseudomonas syringae pv. lachrymans. AU - Fogliano, V.. AU - Gallo, M.. AU - Vinale, F.. AU - Ritieni, A.. AU - Randazzo, G.. AU - Greco, M.. AU - Lops, R.. AU - Graniti, A.. PY - 1999/11. Y1 - 1999/11. N2 - Several strains of plant pathogenic Pseudomonas are known to produce phytotoxic lipodepsipeptides (syringomycin, syringopeptins and related compounds) in vitro. However, detection of these compounds in organic extracts from diseased plant tissues has been attempted by chromatographic methods for syringomycin only. A macromolecular derivative of syringopeptins (KLH-SP(25A+B)) was used to raise polyclonal antibodies in rabbit. The antiserum was able to recognize free syringopeptins (SP22 and SP25) with an estimated detection limit of 0.05 μg per well in the indirect ELISA, and 0.01 μg per well in the competitive ELISA. Cross-reaction with other structurally related lipodepsipeptides, e.g. syringomycins and pseudomycin A, ...

An examination of the results of phylogenetic analyses based on the sequences of fragments of the 16S rRNA, gyrB and rpoD genes, and the discrimination of genomovars based on siderophore diversity within the genus Pseudomonas, has added important taxonomic tools in the characterization of Pseudomonas stutzeri. Eighteen reference strains, nine newly identified hydrocarbon-degrading strains and three strains showing relevant physiological characteristics of P. stutzeri, together with the type strains of four related species, were included in the study. A novel genomovar within the species is described. A summary of the methodology used in these studies and the results of our attempts to define a solid internal subdivision of this important species within the genus Pseudomonas are presented and discussed.

TY - THES. T1 - Genetic aspects of resistance to Pseudomonas solanacearum E.F. Smith in potato. AU - Tung, P.X.. N1 - WU thesis 1506 Proefschrift Wageningen. PY - 1992. Y1 - 1992. N2 - ,TT,The genetic control of resistance toPseudomonas s,TT,olanacearum in potato is complex and may involve both genes with major effects and genes with minor effects. However, no evidence of a gene-for-gene relationship between the host and the pathogen has been as yet documented. Strain specificity in the potato-P. solanacearum pathosystem is of the polygenic quantitative type and is probably a reflection of differential adaptation of host genotype and pathogen genotype to environments. Thus the resistance is chacterized by strong host x pathogen x environment interaction and tends to break down whenever faced with environmental conditions the host is not well adapted to. Expression of the resistance is heavily dependent on the adaptive potential of the carrier host genotype to a particular environment. Under heat ...

Bacteria that inhabit the rhizosphere of agricultural crops can have a beneficial effect on crop growth. One such mechanism is the microbial-driven solubilisation and remineralisation of complex forms of phosphorus (P). It is known that bacteria secrete various phosphatases in response to low P conditions. However, our understanding of their global proteomic response to P stress is limited. Here, exoproteomic analysis of Pseudomonas putida BIRD-1 (BIRD-1), Pseudomonas fluorescens SBW25 and Pseudomonas stutzeri DSM4166 was performed in unison with whole-cell proteomic analysis of BIRD-1 grown under phosphate (Pi) replete and Pi deplete conditions. Comparative exoproteomics revealed marked heterogeneity in the exoproteomes of each Pseudomonas strain in response to Pi depletion. In addition to well-characterised members of the PHO regulon such as alkaline phosphatases, several proteins, previously not associated with the response to Pi depletion, were also identified. These included putative ...

ADP-ribosylation of proteins occurs in many eukaryotes, and it is also the mechanism of action of a growing number of important bacterial toxins. To date, however, there is only one well-characterized ADP-ribosylation system where the ADP-ribosyltransferase and the substrate protein are both bacterial in origin, namely within the nitrogen-fixing bacterium Rhodospirillum rubrum. The present paper demonstrates the endogenous ADP-ribosylation of two proteins of Mr 32,000 and 20,000 within Pseudomonas maltophilia, a Gram-negative aerobe. The proteins have been partially purified: two apparently separate species of modified protein can be separated by ion-exchange chromatography and gel filtration (V0 and Mr 158,000 - Vi). The substrate protein(s) either has, or is co-eluted with, NAD+ glycohydrolase activity. The modification is mono-ADP-ribosyl in nature. The linkage between the acceptor amino acid and the ADP-ribose moiety is alkali-labile and stable to hydroxylamine, possibly indicating an ...

Pseudomonas chlororaphis ATCC ® 55670™ Designation: TX-1 TypeStrain=False Application: Biological control of turfgrass pathogens Biological control of Sclerotinia homoeocarpa

Wang, Dongping; Dorosky, Robert; Han, Cliff; Lo, Chien-chi; Dichosa, Armand; Chain, Patrick; Jun Myoung Yu; Pierson, Leland; III; Pierson, Elizabeth (2015). Adaptation Genomics of a Small-Colony Variant in a Pseudomonas chlororaphis 30-84 Biofilm. Applied and Environmental Microbiology. Available electronically from http : / /hdl .handle .net /1969 .1 /182425. ...

Biodegradation in water: Experimental study and predicted data for the target compound (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one (CAS No. 89-80-5) and varioussupporting studiesfor its structurally similar read across substance were reviewed for the biodegradation end point which are summarized as below: In an experimental key study from peer reviewed journal (1995), biodegradation experiment was conducted for 21 days for evaluating the percentage biodegradability of test substance (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one (CAS no. 89-80-5) by using Pseudomonas citronellolis DSM 50332 as an inoculum. Test inoculum Pseudomonas citronellolis DSM 50332 was obtained from the Deutsche Sammlung von Mikroorganismen, Braunschweig, Germany. Initial test substance conc. used for the study was 308.5 mg/l (2 mM). Anoxic media was used for the study.The medium contained (per liter of distilled water) 1 g of NaCl, 0.1 g of MgCl2.7H2O, 0.04 g of CaCl2, 0.5 g of KCl, 0.125 g of NH4Cl, 0.2 g of ...

This site uses Akismet to reduce spam. 2. In many cases, Pseudomonas infections are preventable. Septicaemia People with existing lung diseases sometimes carry the bacteria in their lungs without causing infection. A doctor may also prescribe an antibiotic called polymyxin. Certain symptoms depend on the site at which the disease occurs. Produce acid from xylose. Pseudomonas bacteria are generally harmless. These include ear infections and skin rashes, especially after exposure to water. Although it rarely causes disease in healthy individuals, it is a significant threat to hospitalized patients, especially those with severe underlying conditions such as cancer and burns. Of the many different types of Pseudomonas, the one that most often causes infections in humans is called Pseudomonas aeruginosa, which can cause infections in the blood, lungs (pneumonia), or other parts of the body after surgery. It is a widespread free-living bacterium and is found in most moist environments such as skin ...

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General Information: Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. This organism is an opportunistic human pathogen. While it rarely infects healthy individuals, immunocompromised patients, like burn victims, AIDS-, cancer- or cystic fibrosis-patients are at increased risk for infection with this environmentally versatile bacteria. It is an important soil bacterium with a complex metabolism capable of degrading polycyclic aromatic hydrocarbons, and producing interesting, biologically active secondary metabolites including quinolones, rhamnolipids, lectins, hydrogen cyanide, and phenazines. Production of these products is likely controlled by complex regulatory ...

Pseudomonas syringae pv. DC3000 is a gram-negative bacterium that infects the model plant Arabidopsis thaliana. Pathogenicity is achieved via secretion of effector proteins into the host cytoplasm through a Type III Secretion System (T3SS). In Ps. DC3000 the T3SS (and associated effector proteins) are dependent on HrpL for their transcription. hrpL transcription is sigma54-dependent and requires two co-dependent enhancer binding proteins, HrpR and HrpS (HrpRS), for activation. HrpRS are regulated by two hrpL-dependent proteins, HrpV and HrpG, where HrpV negatively affects HrpRS activity and HrpG relieves this repression. Here the mechanism of HrpV and HrpGs action on HrpRS activity was tested in vivo and in vitro; and the molecular determinants of HrpV and HrpG functionality were characterised by in silico and mutational analysis. Whole-gene deletion mutants of hrpV and hrpG in Ps. DC3000 revealed complications associated with inserting marker cassettes in transcriptionally-antagonistic ...

Autor: Gupta, K. J. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2013; Open Access; Titel: The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco

Author: Gupta, K. J. et al.; Genre: Journal Article; Published in Print: 2013; Open Access; Title: The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco

General Information: Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. This organism is highly pathogenic for a variety of insects in both larvae and adults. It was isolated from fruit flies and decaying fruits taken from a sample set obtained from the Island of Guadeloupe and tested for induction of a systemic immune response in Drosophila. Destruction of the insect gut tissue occurs during infection.Analysis of the proteins encoded by the genome indicated a number of potential virulence factors, although a type III secretion system was not found. ...

This invention relates to the production and use of recombinant Pseudomonas-derived toxins modified to increase their toxicity and potency in therapy. More particularly, the invention relates to certain deletions in domain II of the amino acid sequence of Pseudomonas exotoxin the domain which relates to the toxins natural proteolytic processing.

The enzyme is involved in production of the rare amino acid 3-methylarginine, which is used by the epiphytic bacterium Pseudomonas syringae pv. syringae as an antibiotic against the related pathogenic species Pseudomonas syringae pv. glycinea ...

Coloured scanning electron micrograph (SEM) of Pseudomonas putida, Gram-negative, aerobic, enteric, rod prokaryote. Pseudomonas putida is a ubiquitous soil bacterium. Strains of P. putida have the ability to degrade organic solvents or hydrocarbons. This strain was isolated from a soil environment that had high levels of caffeine. It is known to biodegrade caffeine. Thus different strains of Pseudomonas putida can be used for bioremediation. Magnification: x1,600 when shortest axis printed at 25 millimetres. - Stock Image C032/1985

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Looking for online definition of Pseudomonas acidovorans in the Medical Dictionary? Pseudomonas acidovorans explanation free. What is Pseudomonas acidovorans? Meaning of Pseudomonas acidovorans medical term. What does Pseudomonas acidovorans mean?

Looking for Pseudomonas pseudomallei? Find out information about Pseudomonas pseudomallei. A bacteria that is the causative agent of melioidosis, an endemic glanders-like disease of humans and animals that occurs most frequently in southeastern... Explanation of Pseudomonas pseudomallei

Taxobox , color = lightgrey , name = Pseudomonas , image = Pseudomonas aeruginosa 01.jpg , image_width = 280px , image_caption = P. aeruginosa colonies on an [[agar plate]]. , regnum = [[Bacterium,Bacteria]] , phylum = [[Proteobacteria]] , classis = [[Proteobacteria,Gamma Proteobacteria]] , ordo = [[Pseudomonadales]] , familia = [[Pseudomonadaceae]] , genus = Pseudomonas , genus_authority = Migula 1894 , type_species = [[Pseudomonas aeruginosa]] , subdivision_ranks = Species , subdivision = P. aeruginosa group :[[Pseudomonas aeruginosa,P. aeruginosa]] :[[Pseudomonas alcaligenes,P. alcaligenes]] :[[Pseudomonas anguilliseptica,P. anguilliseptica]] :[[Pseudomonas argentinensis,P. argentinensis]] :[[Pseudomonas borbori,P. borbori]] :[[Pseudomonas citronellolis,P. citronellolis]] :[[Pseudomonas flavescens,P. flavescens]] :[[Pseudomonas mendocina,P. mendocina]] :[[Pseudomonas nitroreducens,P. nitroreducens]] :[[Pseudomonas ...

Fingerprint Dive into the research topics of Draft genome sequence of Pseudomonas citronellolis LA18T, a bacterium that uses levulinic acid. Together they form a unique fingerprint. ...

TY - JOUR. T1 - Production of medium-chain length polyhydroxyalkanoates by Pseudomonas citronellolis grown in apple pulp waste. AU - Rebocho, Ana Teresa. AU - Pereira, Joao R.. AU - Freitas, Filomena. AU - Neves, Luisa A.. AU - Alves, Vitor D.. AU - Sevrin, Chantal. AU - Grandfils, Christian. AU - Reis, Maria A. M.. N1 - info:eu-repo/grantAgreement/FCT/5876/147258/PT# info:eu-repo/grantAgreement/FCT/5876/147218/PT# info:eu-repo/grantAgreement/FCT/5876/135920/PT# This work was supported by the Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), Associated Laboratory for Sustainable Chemistry - Clean Processes and Technologies (LAQV) and LEAF-Linking Landscape, Environment, Agriculture and Food, which are financed by national funds from FCT/MEC (UID/Multi/04378/2013, UID/QUI/50006/2013 and PEst-OE/AGR/UI0245/2014, respectively) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728, POCI-01-0145-FEDER-007265, respectively), and exploratory project grants ...

A search was undertaken to screen microorganisms in soil which produce an enzyme capable of deacylating glutaryl-7-aminocephalosporanic acid (glutaryl-7-ACA) to 7-aminocephalosporanic acid (7-ACA). To facilitate screening, a model substrate, glutaryl-p-nitroanilide, and a 7-ACA sensitive strain, Enterobacter taylorae BY312, were used as a color indicator and bioassay, respectively. An isolate, Pseudomonas cepacia BY21, was found to produce glutaryl-7-ACA acylase, of which the activity was optimal at pH 8.0 and 45 °C.

The genus Pseudomonas is one of the best-studied bacterial groups in soil, and includes numerous species of environmental interest. Pseudomonas species play key roles in soil, for instance in biological control of soil-borne plant pathogens and in bioremediation of pollutants. A polymerase chain reaction-denaturing gradient gel electrophoresis system that specifically describes the diversity of Pseudomonas spp. in soil was developed. On the basis of this molecular method as well as cultivation-based approaches, the diversity of Pseudomonas species in soil under different agricultural regimes (permanent grassland, arable land either under rotation or under monoculture of maize) was studied. Both types of approaches revealed differences in the composition of Pseudomonas populations between the treatments. Differences between the treatments were also found based on the frequency of isolation of Pseudomonas strains with antagonistic properties against the soil-borne pathogen Rhizoctonia solani AG3. ...

TY - JOUR. T1 - The siderophore pyoverdine of Pseudomonas syringae pv. tabaci 6605 is an intrinsic virulence factor in host tobacco infection. AU - Taguchi, Fumiko. AU - Suzuki, Tomoko. AU - Inagaki, Yoshishige. AU - Toyoda, Kazuhiro. AU - Shiraishi, Tomonori. AU - Ichinose, Yuki. PY - 2010/1. Y1 - 2010/1. N2 - To investigate the role of iron uptake mediated by the siderophore pyoverdine in the virulence of the plant pathogen Pseudomonas syringae pv. tabaci 6605, three predicted pyoverdine synthesis-related genes, pvdJ, pvdL, and fpvA, were mutated. The pvdJ, pvdL, and fpvA genes encode the pyoverdine side chain peptide synthetase III L-Thr-L-Ser component, the pyoverdine chromophore synthetase, and the TonB-dependent ferripyoverdine receptor, respectively. The ΔpvdJ and ΔpvdL mutants were unable to produce pyoverdine in mineral salts-glucose medium, which was used for the iron-depleted condition. Furthermore, the ΔpvdJ and ΔpvdL mutants showed lower abilities to produce tabtoxin, ...

Strain UFB2 was isolated from a soybean field soil in Mississippi and identified as a member of Pseudomonas chlororaphis. Strain UFB2 has a broad-spectrum antimicrobial activity against common soil-borne pathogens. Plate assays showed that strain UFB2 was especially efficient in inhibiting the growth of Clavibacter michiganensis 1-07, the causal agent of the devastating bacterial canker of tomato. Here, the complete genome sequence of P. chlororaphis strain UFB2 is reported and described. The strain UFB2 genome consists of a circular chromosome of 6,360,256 bp of which 87.86 % are protein-coding bases. Genome analysis revealed multiple gene islands encoding various secondary metabolites such as 2,4-diacetylphloroglucinol. Further genome analysis will provide more details about strain UFB2 antibacterial activities mechanisms and the use of this strain as a potential biocontrol agent.

We have measured the in-vitro activity of 27 antimicrobials against 211 clinical and ten reference strains of Pseudomonas pseudomallei. Imipenem was the most active antibiotic tested, followed by piperacillin, doxycycline, amoxycillin/clavulanic acid, cefixime, cefetamet, azlocillin and ceftazidime, all of which had MICs of less than or equal to 2 mg/l for the majority of strains. The measured MICs were dependent on the media and inocula used, to an extent which varied with the antibiotic class under test; MICs of ureidopenicillins were particularly inoculum-dependent. The beta-lactams and ciprofloxacin were bactericidal, whereas the agents conventionally used to treat melioidosis (doxycycline, chloramphenicol, sulphamethoxazole and trimethoprim) had bacteriostatic activity only. Strains highly resistant to chloramphenicol (MIC greater than or equal to 256 mg/l) emerged during treatment in 7.1% of patients. These strains were fully virulent, and frequently showed cross-resistance to tetracyclines,

para-Nitrophenol (PNP) is a highly toxic compound with threats to mammalian health. The pnpE-encoded γ-hydroxymuconic semialdehyde dehydrogenase catalyzes the reduction of γ-hydroxymuconic semialdehyde to maleylacetate in Pseudomonas sp. strain WBC-3, playing a key role in the catabolism of PNP to Krebs cycle intermediates. However, the catalyzing mechanism by PnpE has not been well understood. Here we report the crystal structures of the apo and NAD bound PnpE. In the PnpE-NAD complex structure, NAD is situated in a cleft of PnpE. The cofactor binding site is composed of two pockets. The adenosine and the first ribose group of NAD bind in one pocket and the nicotinamide ring in the other. Six amino acids have interactions with the cofactor. They are C281, E247, Q210, W148, I146 and K172. Highly conserved residues C281 and E247 were identified to be critical for its catalytic activity. In addition, flexible docking studies of the enzyme-substrate system were performed to predict the interactions

A virulent strain of Pseudomonas pseudomallei was subjected to ultraviolet irradiation and a mutant clone requiring preformed adenine or hypoxanthine for growth was isolated. The imposition of auxotrophism was associated with a striking decline in virulence for mice which could be restored by permitting the strain to revert to purine independence in vitro. The mutant persisted in mice without detected reversions or or untoward effects for approximately 20 days following inoculation of 107 cells. After repeated inoculations, a significant immune response was demonstrated by parenteral challenge with diverse strains of this species. However, animals so immunized remained susceptible to lethal respiratory infection.. ...

Yap, E.H.,Thong, T.W.,Tan, A.L.,Yeo, M.,Tan, H.C.,Loh, H.,Teo, T.P.,Thong, K.T.,Singh, M.,Chan, Y.C. (1995). Comparison of Pseudomonas pseudomallei from humans, animals, soil and water by restriction endonuclease analysis.. Singapore Medical Journal 36 (1) : 60-62. ScholarBank@NUS Repository ...

TY - JOUR. T1 - Genome-wide analysis of bacterial determinants of plant growth promotion and induced systemic resistance by Pseudomonas fluorescens. AU - Cheng, Xu. AU - Etalo, Desalegn W.. AU - van de Mortel, Judith E.. AU - Dekkers, Ester. AU - Nguyen, Linh. AU - Medema, Marnix H. AU - Raaijmakers, Jos M.. N1 - 6370, ME; Data Archiving: data ia archived at GEO databank. PY - 2017. Y1 - 2017. N2 - Pseudomonas fluorescens strain SS101 (Pf.SS101) promotes growth of Arabidopsis thaliana, enhances greening and lateral root formation, and induces systemic resistance (ISR) against the bacterial pathogen Pseudomonas syringae pv. tomato (Pst). Here, targeted and untargeted approaches were adopted to identify bacterial determinants and underlying mechanisms involved in plant growth promotion and ISR by Pf.SS101. Based on targeted analyses, no evidence was found for volatiles, lipopeptides and siderophores in plant growth promotion by Pf.SS101. Untargeted, genome-wide analyses of 7,488 random transposon ...

Ring cyclization and eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic-acid to PQQ.

Plants of which the roots are colonized by selected strains of non-pathogenic, fluorescent Pseudomonas spp. develop an enhanced defensive capacity against a broad spectrum of foliar pathogens. In Arabidopsis thaliana, this rhizobacteria-induced systemic resistance (ISR) functions independently of salicylic acid but requires responsiveness to jasmonic acid and ethylene. In contrast ... read more to pathogen-induced systemic acquired resistance (SAR), ISR is not associated with systemic changes in the expression of genes encoding pathogenesis-related (PR) proteins. To identify genes that are specifically expressed in response to colonization of the roots by ISR-inducing Pseudomonas fluorescens WCS417r bacteria, we screened a collection of Arabidopsis enhancer trap and gene trap lines containing a transposable element of the Ac/Ds system and the GUS reporter gene. We identified an enhancer trap line (WET121) that specifically showed GUS activity in the root vascular bundle upon colonization of the ...

Pseudomonas is a genus of bacteria commonly found in investigations of gut microbes in malaria mosquitoes. Among those mosquitoes is the dominating malaria vector in Asia, Anopheles stephensi, where Pseudomonas is a prevailing bacterium and natural inhabitant of its breeding places. In order to explore the reason for finding Pseudomonas so frequently, an investigation of its localization and transstadial properties was undertaken. A Pseudomonas isolate from An. stephensi was transformed successfully with an endogenous plasmid modified to express green fluorescent protein (GFP). Subsequently, the Pseudomonas-GFP was added to the laboratory larval breeding place of An. stephensi and taken up by the larvae. After 24 hours, the larvae were cleaned and moved to a bath with double-distilled water. Also, female adults were fed sugar solution containing Pseudomonas-GFP. The Pseudomonas-GFP was traced in the alimentary canal of larvae, pupae and adults. Fluorescent microscopy and PCR assays

Research Article Silver Nanoparticles as an Effective Anti-Nanobacterial System towards Biofilm Forming Pseudomonas oryzihabitans. Shaimaa Obaid Hasson 1*, Mohammed Jabber Al-Awady 2, Mohanad Jawad Kadhim 2, Hayder Shkhair Al-Janabi 2. 1 Department of Microbiology, College of Veterinary, Al-Qasim Green University, Babylon, Iraq.. 2 Department of Genetic Engineering, Faculty of Biotechnology, Al Qasim Green University, Babylon, Iraq.. * Corresponding author. E-mail: [email protected]. Received: Jun. 18, 2019; Accepted: Aug. 22, 2019; Published: Aug. 23, 2019. Citation: Shaimaa Obaid Hasson, Mohammed Jabber Al-Awady, Mohanad Jawad Kadhim, and Hayder Shkhair Al-Janabi, Silver Nanoparticles as an Effective Anti-Nanobacterial System towards Biofilm Forming Pseudomonas oryzihabitans. Nano Biomed. Eng., 2019, 11(3): 297-305.. DOI: 10.5101/nbe.v11i3.p297-305.. Abstract Silver nanoparticles have been considered a powerful antimicrobial agents recently especially after increasing incidence of ...

Inhibitory effect of commercial antibiotics and bioactive compounds produced by the isolate W3 against vibrios. Most of the antibiotics used could cause inhibition of all tested strains of V. harveyi (Table 1), Hence antibiotics could be used to control V. harveyi infections that affect aquaculture in Thailand. However, some of them such as chloramphenicol, furazolidone, tetracycline and oxonilic acid are now banned for use in aquaculture as previously mentioned. In general, β-lactam antibiotics such as ampicillin are preferred for use by shrimp farmers to treat luminous vibriosis disease since these groups of antibiotics do not cause significant side effects (Teo et al. 2002). Unfortunately, all tested strains in this study were resistant to ampicillin and this result is in agreement with Teo et al. (2002) who reported that many types of β-lactam antibiotics were no longer able to prevent vibriosis. In Thailand sulphamethoxazole is normally used in hatcheries against Vibrio sp.; however, in ...

A nitrogen-fixing bacterium, designated strain 6H33bT, was isolated from a compost pile in Japan. The nitrogenase activity of this strain was detected based on its acetylene-reducing activity under low oxygen concentrations (2-4 %). An analysis of the genes responsible for nitrogen fixation in this strain, nifH and nifD, indicated a close relationship to those of Pseudomonas stutzeri A15 (A1501). Sequence similarity searches based on the 16S rRNA gene sequences showed that strain 6H33bT belongs within the genus Pseudomonas sensu stricto; closest similarity was with Pseudomonas indica (97·3 %). A comparison of several taxonomic characteristics of 6H33bT with those of P. indica and some type strains of the genus Pseudomonas sensu stricto indicated that 6H33bT could be distinguished from P. indica based on the presence of nitrogen fixation ability, the absence of nitrate reduction and denitrification abilities and the utilization of some sugars and organic acids. Phylogenetic analyses and the results of

Domain architectures containing the following SCOP superfamilies _gap_,100950,_gap_ in Pseudomonas mendocina NK-01. Domain architectures illustrate each occurrence of _gap_,100950,_gap_.

Cloning and identification of genes involved in the interaction between the bacterial stone fruit pathogen Pseudomonas syringae PV.syringae strain NV and plum ...

Arnold, D. L., Lovell, H., Jackson, R. and Mansfield, J. W. (2011) Pseudomonas syringae pv. phaseolicola: From has bean to supermodel. Molecular Plant Pathology , 12 (7). pp. 617-627. ISSN 1464-6722 Available from: http://eprints.uwe.ac.uk/14811 Godfrey, S., Lovell, H., Mansfield, J. W., Corry, D., Jackson, R. W. and Arnold, D. L. (2011) The stealth episome: Suppression of gene expression on the excised genomic island PPHGI-1 from Pseudomonas syringae pv. phaseolicola. PLoS Pathogens, 7 (3). ISSN 1553-7366 Available from: http://eprints.uwe.ac.uk/14583 Lovell, H., Jackson, R. W., Mansfield, J. W., Godfrey, S. A. C., Hancock, J. T., Desikan, R. and Arnold, D. L. (2011) In planta conditions induce genomic changes in Pseudomonas syringae pv. phaseolicola. Molecular Plant Pathology, 12 (2). pp. 167-176. ISSN 1464-6722 Available from: http://eprints.uwe.ac.uk/11468 Godfrey, S., Mansfield, J. W., Corry, D., Lovell, H., Jackson, R. and Arnold, D. L. (2010) Confocal imaging of pseudomonas syringae pv. ...

The structural gene (hdl IVa) for the Pseudomonas cepacia MBA4 2-haloacid halidohydrolase IVa (Hdl IVa) was isolated on a 1.6 kb fragment of Ps. cepacia MBA4 chromosomal DNA. The recombinant halidohydrolase was expressed in Escherichia coli and Pseudomonas putida and the structural gene was subcloned on to the tac expression vector pBTac1. High-level expression from the tac promoter was seen to be temperature-dependent, a consequence of the nucleotide sequence adjacent to the fragment encoding the halidohydrolase. The nucleotide sequence of the fragment encoding the Hdl IVa was determined and analysed. Three ATG codons were identified in one of the open reading frames and the one corresponding to the start of the hdl IVa structural gene was determined by comparison of the predicted amino acid sequences with the experimentally determined N-terminal sequences of halidohydrolase IVa. The hdl IVa gene encoded a 231-amino acid-residue protein of M(r) 25,900. The sequence and predicted structural data ...

The nucleotide sequence of a 4.5-kilobase copper resistance determinant from Pseudomonas syringae pv. tomato revealed four open reading frames (ORFs) in the same orientation. Deletion and site-specific mutational analyses indicated that the first two ORFs were essential for copper resistance; the last two ORFs were required for full resistance, but low-level resistance could be conferred in their absence. Five highly conserved, direct 24-base repeats were found near the beginning of the second ORF, and a similar, but less conserved, repeated region was found in the middle of the first ORF. ...

In the last decade, the worldwide production of kiwi fruit has been highly affected by Pseudomonas syringae pv. actinidiae (Psa), a phytopathogenic bacterium; this has led to severe economic losses that are seriously affecting the kiwi fruit trade. The available treatments for this disease are still scarce, with th

The phyllosphere is known for hosting a great diversity of fungi, yeasts and bacteria. These microorganisms interact with each other and with the host plant in the form of symbiosis, mutualism, commensalism, parasitism, competition or simply neutralism.|em| Pseudomonas syringae|/em| is a ubiquitous epiphytic bacterium commonly found in these microbial communities. The phylogeny complex of |em|P. syringae|/em| comprises 13 phylogroups, containing strains that are well-known pathogens and strains that apparently have limited capacity as pathogens. Emblematic among the pathogens are |em|P. syringae|/em| pv.|em| syringae|/em| (Pss) and |em|P. syringae|/em| pv. |em|actinidiae|/em| (Psa) belonging respectively to phylogroups 2 and 1. Bacterial blights of fruit trees caused by |em|P. syringae|/em| lead to significant economic losses worldwide. With the expansion of bacterial blight of kiwifruit caused by P. syringae pv. actinidiae and bacterial blight of apricot caused by |em|P. syringae|/em| pv. |em|syringae|

Pseudomonas putida is a Gram-negative, rod-shaped, saprotrophic soil bacterium. Based on 16S rRNA analysis, P. putida was taxonomically confirmed to be a pseudomonas species (sensu stricto) and placed, along with several other species, in the P. putida group, to which it lends its name.,ref,{{#invoke:Citation/CS1,citation ,CitationClass=journal }},/ref, A variety of P. putida, called multi-plasmid hydrocarbon-degrading Pseudomonas, is the first patented organism in the world. Because it is a living organism, the patent was disputed and brought before the United States Supreme Court in the historic court case Diamond v. Chakrabarty which the inventor, Ananda Mohan Chakrabarty, won. It demonstrates a very diverse metabolism, including the ability to degrade organic solvents such as toluene.,ref,{{#invoke:Citation/CS1,citation ,CitationClass=journal }},/ref, This ability has been put to use in bioremediation, or the use of microorganisms to biodegrade oil. Use of P. putida is preferable to some ...

TY - JOUR. T1 - Immobilization of Pseudomonas sp. strain ADP. T2 - Applied Clay Science. AU - Stelting, Scott. AU - Burns, Richard G.. AU - Sunna, Anwar. AU - Visnovsky, Gabriel. AU - Bunt, Craig R.. PY - 2012/8. Y1 - 2012/8. N2 - Storage and delivery of beneficial microorganisms are fundamental issues determining their value and effectiveness for a wide range of industrial and environmental purposes. One such application is the use of bacteria for the remediation of soil pollutants such as polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and persistent pesticides. Liquid cultures of a candidate for atrazine degradation in soil and water, Pseudomonas sp. strain ADP (3.67×10 9 colony-forming units, cfu/mL), when stored at 4 and 25°C, showed a 1 log reduction in cfu/mL occurs after approximately 4 and 2weeks, respectively. When immobilized onto natural zeolite from two sources (a New Zealand and an Australian quarry) and stored in open containers exposed to the ...

Citation: Funnell-Harris, D.L., Sattler, S.E., Pedersen, J.F. 2013. Characterization of fluorescent Pseudomonas spp. associated with roots and soil of two sorghum genotypes. European Journal of Plant Pathology. 136 (3): 469-481. Interpretive Summary: Fluorescent Pseudomonas bacteria were collected from roots and associated soil of different sorghum cultivars and a wheat cultivar grown in two different soil types. The ability of the bacteria to produce compounds that inhibit fungi that cause diseases in sorghum was determined. Results indicated that both soil type and plant cultivar affected soil bacteria populations that can inhibit sorghum pathogens. Two bacterial isolates or strains found on the sorghum roots and associated soil inhibited the growth of five pathogens. These two isolates have potential as biological control agents for sorghum fungal pathogens. Technical Abstract: Sorghum is used as bioenergy feedstock, animal feed, and food. Economical methods for disease prevention and control ...

Pseudomonas putida is a well-known plant growth promoting bacterium (PGPB) with the capacity to improve plant growth. The objective of this study was to assess the effect of P. putida towards plant growth and its interaction with its microbial ecosystem. P. putida strain PF1P was isolated from the rhizosphere of banana plant (Musa species). The isolated P. putida strain PF1P possess ability to secrete acetic acid (42.1 µg/mL) and gibberellic acid (10.1 µg/mL). Further study conducted showed that P. putida strain PF1P was able to increase 54.6% of fresh plant weight and 51.3% of root length for Brassica chinensis var parachinensis. Soil microbial assessment indicates that P. putida strain PF1P did not have any detrimental effects as Shannon diversity index showed an increase in the microbial diversity from 2.635 to 2.903 with the colony forming unit per gram of soil increase from 9 × 107 to 5 × 109, respectively for control and treated soil. Soil health check also showed that

The potential of the thymidylate synthase thyA gene cloned from Lactococcus lactis subsp. lactis as a possible alternative selectable marker gene to antibiotic resistance markers has been examined. The thyA mutation is a recessive lethal one; thyA mutants cannot survive in environments containing low amounts of thymidine or thymine (such as Luria-Bertani medium) unless complemented by the thyA gene. The cloned thyA gene was strongly expressed in L. lactis subsp. lactis, Escherichia coli, Rhizobium meliloti, and a fluorescent Pseudomonas strain. In addition, when fused to a promoterless enteric lac operon, the thyA gene drove expression of the lac genes in a number of gram-negative bacteria. In transformation experiments with thyA mutants of E. coli and conjugation experiments with thyA mutants of R. meliloti, the lactococcal thyA gene permitted selection of transformants and transconjugants with the same efficiency as did genes for resistance to ampicillin, chloramphenicol, or tetracycline. ...

Pseudomonas putida strain MTCC5279 is a plant-growth-promoting rhizobacterium (PGPR) isolated from the roots of chick-pea (Cicer arietinum L. cv. Radhey) in a rain-fed area of Dholpur, Rajasthan, India. The strain P. putida MTCC5279 displayed plant growth promotional attributes such as the presence of auxin and the ability to solubilize phosphate and showed drought and salt tolerance (1).. Here we present the genome of P. putida MTCC5279 sequenced using a Roche Genome Sequencer FLX 454 system. A total of 237,769 reads were generated, covering a length of 78.2 Mb. These reads were assembled at an overlap size of 40 bp and 96% identity using the GS Assembler program, which yielded 193 contigs (longest contig, 152 kb; average contig, 27 kb). The consensus length of the draft genome sequence is 5.2 Mb, with ~14× coverage and a total GC content of 62.5%. The closest neighbor among its relatives was P. putida strain KT2440, with a genome size of about 6.18 Mb and GC content of 61.5% (2). Gene ...

Pseudomonas fluorescens BRG100 produces secondary metabolites with herbicidal activity to the grass weeds wild oat, Avena fatua, and green foxtail, Setaria viridis. The green fluorescence protein (gfp) gene was introduced into P. fluorescens BRG100 from Escherichia coli S17-1¥ë via a Tn5 mini transposon suicide vector system. Colony morphology, growth rate in liquid media, weed biocontrol efficacy (plant growth pouch), carbon utilization (Biolog GN) and root colonization of green foxtail by several P. fluorescens BRG100gfp transformants were determined to be the same as the wild type. Pseudomonas fluorescens BRGgfp-15 was found to be most similar to the wild-type in all of the above characteristics and was thus used in subsequent experiments. Note: all strains of Pseudomonas fluorescens will be referred to by only their strain throughout (ie. BRGgfp-15 and BRG100). It was determined by population dynamics per section of root with spiral plating on culture medium, epi-fluorescence and confocal ...

The cloned 9.4-kb insert of plasmid pNHJ20L containing low-molecular-massnitrile hydratase (L-NHase) gene from Rhodococcus rhodochrous J1[Kobayashi, M. et al. (1991) Biochim. Biophys. Acta 1129, 23-33] wasdigested with various restriction enzymes, and the trimmed fragments wereinserted into pUC18 or pUC19. A 1.96-kb EcoRI-SphI region located 1.9-kbdownstream of the L-NHase gene was found to be essential for theexpression of amidase activity in Escherichia coli; the gene arrangementof the amidase and the NHase in R. rhodochrous J1 differed from those inRhodococcus species including N-774 and Pseudomonas chlororaphis B23. Thenucleotide-determined sequence indicated that the amidase consists of 515amino acids (54626 Da) and the deduced amino acid sequence of the amidasehad high similarity to those of amidases from Rhodococcus speciesincluding N-774 and P. chlororaphis B23 and to indole-3-acetamidehydrolase from Pseudomonas savastanoi. The amidase gene modified in thenucleotide sequence upstream ...

Pseudomonas syringae CmaA protein: involved in coronamic acid biosynthesis in Pseudomonas syringae; amino acid sequence given in first source; GenBank U14657

The bacterial plant pathogen Pseudomonas syringae causes economically important diseases of a wide variety of plant species and is used as a model organism to understand the molecular basis of plant disease. Much existing research into P. syringae-plant interactions has focused on the molecular basis of plant disease resistance and the role of secreted effector proteins in the suppression of plant defences. However, researchers have speculated that the diverse array of effectors, toxins and hormones produced by this pathogen also play an important role in manipulating plant metabolism to promote infection. Recent advances in metabolomics, genomics, transcriptomics and metabolic modelling offer new opportunities to address this question and generate a system-level understanding of metabolic interactions at the host-pathogen interface.

Background|br /|The genetic network of the TOL plasmid pWW0 of the soil bacterium Pseudomonas putida mt-2 for catabolism of m-xylene is an archetypal model for environmental biodegradation of aromatic pollutants. Although nearly every metabolic and transcriptional component of this regulatory system is known to an extraordinary molecular detail, the complexity of its architecture is still perplexing. To gain an insight into the inner layout of this network a logic model of the TOL system was implemented, simulated and experimentally validated. This analysis made sense of the specific regulatory topology out on the basis of an unprecedented network motif around which the entire genetic circuit for m-xylene catabolism gravitates.|br /|Results|br /|The most salient feature of the whole TOL regulatory network is the control exerted by two distinct but still intertwined regulators (XylR and XylS) on expression of two separated catabolic operons (upper and lower) for catabolism of m-xylene. Following model

Looking for Pseudomonas aeruginosa? Find out information about Pseudomonas aeruginosa. An opportunistic pathogen that is the most significant cause of hospital-acquired infections, particularly in predisposed patients with metabolic,... Explanation of Pseudomonas aeruginosa

TY - JOUR. T1 - The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor. AU - Kim, Kyeong Kyu. AU - Song, Hyun Kyu. AU - Shin, Dong Hae. AU - Hwang, Kwang Yeon. AU - Sun, Se Won. PY - 1997/2/15. Y1 - 1997/2/15. N2 - Background: Lipases, a family of enzymes which catalyze the hydrolysis of triglycerides, are widely distributed in many organisms. True lipases are distinguished from esterases by the characteristic interfacial activation they exhibit at an oil-water interface. Lipases are one of the most frequently used biocatalysts for organic reactions performed under mild conditions. Their biotechnological applications include food and oil processing and the preparation of chiral intermediates for the synthesis of enantiomerically pure pharmaceuticals. Recent structural studies on several lipases have provided some clues towards understanding the mechanisms of hydrolytic activity, interfacial activation, and ...

Pseudomonas putida 3SK, a solvent resistant strain, produced extracellular lipase. We observed that there was another lipolytic enzyme, which had no lipase activity in P. putida 3SK. This lipolytic enzyme, an esterase, was cloned in Escherichia coli and sequenced. The cloned esterase gene, which designated estA, had single open reading frame of 1941 nucleotides. The estA gene encoded a protein of 646 amino acids and was predicted to belong to the GDSL family of lipolytic enzymes. The consensus motif GxSxxDxG, which was a characteristic feature of the GDSL lipolytic enzymes, was found in estA. A putative catalytic triad, $Ser^{38}$, $Asp^{172}$, and $His^{313}$ was also determined. The calculated molecular weight was 69.6kDa and theoretical isoelectric point (pI) was 4.68. The esterase expressed in E. coli was purified by Ni-NTA affinity chromatography and FPLC gel filtration chromatography. The molecular weight of the purified esterase was about 65kDa and judging from the immunoblot analysis, an ...