Pseudomonas aeruginosa bloodstream infection (BSI) is predominantly acquired in the hospital setting. Community-onset infection is less common. Differences in epidemiology, clinical features, microbiological factors and BSI outcomes led to the separation of bacterial community-onset BSI into the categories of healthcare-associated infection (HCAI) and community-acquired infection (CAI). Community-acquired P. aeruginosa BSI epidemiology is not well defined in the literature. In addition, it is also not clear if the same factors separate CAI and HCAI BSI caused by P. aeruginosa alone. A retrospective multicentre cohort study was performed looking at P. aeruginosa BSI from January 2008 to January 2011. Strict definitions for HCAI and CAI were applied. Extensive epidemiological, clinical and outcome data were obtained. Thirty-four CAI episodes and 156 HCAI episodes were analysed. The CAI group could be characterised into seven distinct categories based on comorbidities and clinically suspected ...
Pseudomonas infections are diseases caused by a bacterium from the genus Pseudomonas. The bacteria are found widely in the environment, such as in soil, water, and plants. They usually do not cause infections in healthy people. If an infection does occur in a healthy person, it is generally mild. More severe infections occur in people who are already hospitalized with another illness or condition, or people who have a weak immune system. Pseudomonades are fairly common pathogens involved in infections acquired in a hospital setting. A pathogen is a microorganism that causes disease. Infections acquired in a hospital are called nosocomial infections. Infections can occur in any part of the body. Symptoms depend on which part of the body is infected. Antibiotics are used to treat the infections. Pseudomonas infection could be fatal in people who are already very ill.. ...
This graph shows the total number of publications written about Pseudomonas Infections by people in this website by year, and whether Pseudomonas Infections was a major or minor topic of these publications ...
The ELITE trial shows that treatment with TIS for 28 days is effective for treating early P aeruginosa infection, and extending TIS treatment to 56 days does not provide notable additional improvements. Over 90% of randomised patients in the 28-day and 56-day TIS groups had negative cultures for P aeruginosa 1 month after the end of treatment, and the majority of these patients remained free from infection for up to 27 months. The results were similar in sputum and non-sputum producers and were unaffected by the patients baseline characteristics (age, lung function and first versus recurrent infection).. Antibiotics, either alone or in combination, have been shown to be effective in the treatment of early P aeruginosa infection.3-6 11 14 However, to date there have been no reports of comparative studies assessing different treatment regimens and only a few controlled studies have been performed. In an open controlled trial, inhaled colistin and oral ciprofloxacin twice daily for 3 weeks was ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
CF is an inherited disease that causes mucus to build up in the lungs and digestive tract, which can cause lung infections and digestive problems. It is the most common type of chronic lung disease in children and young adults and may result in early death. There is no cure for this disease. The primary cause of death in individuals with CF is progressive obstructive pulmonary disease associated with chronic Pseudomonas aeruginosa (PA) infection. PA infection can occur early in life and can become highly resistant to antibiotics. Once an individual has been diagnosed with chronic PA infection, it is almost impossible to manage effectively. The need exists for an effective treatment to control and eliminate PA infection. Past research has shown that if PA infection is treated early, there is a greater likelihood that it may be eliminated completely. This study will examine two treatment regimens to compare which is more effective at eliminating PA infection. In the first regimen, participants ...
Sánchez-Diener I1, Zamorano L2, Peña C3, Ocampo-Sosa A4, Cabot G1, Gómez-Zorrilla S5, Almirante B6, Aguilar M7, Granados A8, Calbo E9, Baño JR10, Rodríguez-López F11, Tubau F12, Martínez-Martínez L11, Navas A13, and Oliver A14. 1Servicio de Microbiología and Unidad de Investigación, Hospital Son Espases, Instituto de Investigación Sanitaria de les Illes Balears (IdISBa), Palma de Mallorca,…
Sigma-Aldrich offers abstracts and full-text articles by [Amber L Jolly, Desire Takawira, Olufolarin O Oke, Sarah A Whiteside, Stephanie W Chang, Emily R Wen, Kevin Quach, David J Evans, Suzanne M J Fleiszig].
MBio. 2013 Mar 12;4(2):e00032-13. doi: 10.1128/mBio.00032-13. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
This chapter reviews the observations supporting the idea that acute and chronic Pseudomonas aeruginosa infections represent distinct modes of host-pathogen interaction. The virulence factors associated with acute infections and chronic infections are discussed, with a focus on data obtained from human subject-based studies, when possible. P. aeruginosa expresses many virulence factors that can damage host cells and which contribute to infection in both humans and animal models. Ectopic expression of virulence factor regulator (Vfr) in mucA22 strains restored expression of ExoS, type IV pilus (TFP), and elastase, confirming that downregulation of Vfr is responsible for decreased virulence factor expression in mucoid strains under the conditions evaluated in this study. While it is clear that regulators of TFP biogenesis and function are intimately associated with the control of Vfr and cyclic AMP (cAMP) expression, the mechanism that links twitching motility and Vfr remains to be elucidated. rsmZ and
1. Gomez MI, Prince A. Opportunistic infections in lung disease: Pseudomonas infections in cystic fibrosis. Current opinion in pharmacology. 2007;7(3):244-51. 17418640. 2. Soler N, Torres A, Ewig S, Gonzalez J, Celis R, El-Ebiary M, et al. Bronchial microbial patterns in severe exacerbations of chronic obstructive pulmonary disease (COPD) requiring mechanical ventilation. American journal of respiratory and critical care medicine. 1998;157(5 Pt 1):1498-505. 9603129. 3. Sethi S, Evans N, Grant BJ, Murphy TF. New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. The New England journal of medicine. 2002;347(7):465-71. 12181400. 4. Rada B, Leto TL. Pyocyanin effects on respiratory epithelium: relevance in Pseudomonas aeruginosa airway infections. Trends in microbiology. 2013;21(2):73-81. doi: 10.1016/j.tim.2012.10.004 23140890. 5. Bleves S, Viarre V, Salacha R, Michel GP, Filloux A, Voulhoux R. Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic ...
That research had shown that the bacteria travelled up to 4m and stayed viable for 45 minutes after being coughed into the air, says lead researcher Lidia Morawska in a university press release.. For their experiment, the researchers recruited two individuals- one of whom had cystic fibrosis, and the other of whom had a chronic pseudomonas aeruginosa infection. Airborne cough droplets were collected from both patients, although there was more focus on the patient who was infected.. Despite the droplets of infected saliva quickly losing moisture, contaminated particles remained airborne for quite some time after the infected individual coughed or sneezed, the researchers found. This was particularly the case for larger droplets.. We found that the concentration of active bacteria in the dried droplets showed rapid decay with a 10-second half-life for most of the bacteria but a subset of bacteria had a half-life of more than 10 minutes, reveals Morawska.. LIKE STUDIES? FOLLOW STUDYFINDS.ORG ON ...
A seventh baby is believed to have contracted the Pseudomonas infection at the Royal hospital. A seventh baby is believed to have contracted the Pseudomonas infection at the Royal hospi
Takei Y, however, is often short meidcation so take into account side-effects such as proximal myopathy and poor wound healing. Opal SM, Jhung JW, Keith JC Jr, et al Recombinant human interleukin-11 in experimental Pseudomonas aeruginosa sepsis in immunocompromised animals. The size of the system and the amount of in-house (vs.
Individuals with mild to moderate disease typically can be treated with an intermittent regimen of antibiotics daily or three times per week. Infection with novel SARS-CoV-2 carries significant morbidity and mortality in patients with pulmonary compromise, such as lung cancer, autoimmune disease, and pneumonia. However, people with chronic lung problems or people with weak immune systems may be at greater risk for developing the infection. What is a Lung Infection? Treating Pseudomonas aeruginosa lung infections. MAC lung disease treatment usually involves a combination of … Little is known regarding response of antimicrobial agents and clinical outcome for this rare species. Treatment with a combination of antibiotic drugs (drug regimen) is the mainstay of therapy for these diseases. Pulmonary infections are common and are caused by a wide range of organisms.. Mycobacterium abscessus infections are challenging to treat because multidrug resistance necessitates prolonged intravenous (IV) ...
Introduction: Changes in the prevalence of respiratory pathogens in CF may reflect improved therapeutic strategies and clinical practice within a CF centre.. Aims: We hypothesized that active microbiological surveillance and a low threshold for long term nebulised antibiotics might reduce the prevalence of respiratory pathogens in patients with CF.. Methods: Retrospective review of data of patients under full care at a paediatric CF centre in Cardiff between 1998(n=80) and 2011(n=70). We calculated the number of isolates for common pathogens from 1998 onward (expressed as a percentage for each year); mean number of respiratory cultures taken for each patient per year; and the rate of chronic P aeruginosa (Lee 2003) from 2002 onward. Changes in prevalence over time were assesed by linear regression.. Results:Non-significant increase in mean (SD) number of respiratory cultures from 5.3(3.22) to 7.4(2.89) per patient/year.The prevalence of P aeruginosa infection decreased significantly from 43.8% ...
About 80 % of CF patients develop chronic P. Aeruginosa lung infection and antibiotic resistance is a growing problem. 'New classes of inhaled
Community acquired pneumonia (CAP) is a major cause of morbidity and mortality worldwide. The contribution of Pseudomonas aeruginosa to both disease burden and antibiotic resistance is unclear; Restrepo et al (European Respiratory Journal 2018;52: 1701190) present a point-prevalence study to address this gap in the data. This multinational study of hospitalised patients investigated the prevalence, risk factors and antibiotic resistance profiles of P. aeruginosa-CAP in 54 countries. A total of 3193 patients were included, all had microbiological testing on admission and had a confirmed diagnosis of CAP. Prevalence of P. aeruginosa-CAP was 4.2 % (133/3193) which represented 11.3 % (133/1173) of all patients with a positive bacterial culture result. Almost half of these patients had an antibiotic-resistant strain (64/133, 48 %; 2.0 % of total cohort) and a quarter had a multi-drug resistant strain of P. aeruginosa (33/133, 25 %; 1.0 % of total cohort). Prior Pseudomonas infection/colonisation (OR ...
People with weakened immune systems are susceptible to pseudomonas infections. Luella May shares safe, effective alternatives to Big Pharma antibiotics ...
Throat cultures werent as good this time. She had 2 clean cultures since her first pseudomonas infection last April. I was really hoping for a 3rd clean culture, but luck was not on our side. Her lungs are culturing MRSA, so were going to do a round of bactrum and see if it can eradicate it. The nurse coordinator explained that, just like with the pseudos, they want to try to catch and treat a MRSA infection quickly so it doesnt become a more serious issue and so they can reduce the amount of potential damage it can do to the lungs. I did give her a heads up about Judiths 2 MRSA infections in the abscesses over the summer, just in case they wanted to move to a stronger antibiotic. Since the cultures for the lungs are slightly different than the skin cultures, she said they can still try the bactrum and take it from there. One good point we discovered from the culture is she is still pseudo-free, so at least we dont have to worry about that on top of the MRSA ...
Hi all, so my 6 years old cat is been treated with an Antibiotic for the treatment of Pseudomonas infection. Does your vet advise you to always give your...
My senior year, he developed an intramuscular sarcoma that appeared very histologically aggressive. He had an entire muscle in his leg removed, a large skin graft, got a Pseudomonas infection, spent a week in the hospital. He was a great patient until you put him in a kennel. He broke out of the ICU and went wandering to find the technician in the night. He developed a slough from extravascular administration of antibiotics in his other front leg and wore two full leg bandages for weeks. In July after graduation and months after surgery, he threw a clot to his front leg and I thought we were toast, but he recovered after 24 hours in the hospital again. He never was aggressive or even resistant to treatment, but by the end of vet school he hated hospitals. I promised him then Id never leave him in one again ...
The philosophy in traditional drug design, i.e., the one gene, one drug, one disease paradigm, focuses on the individual properties of a protein, for example, whether the deletion of a gene is lethal to an organism and if it is, then inhibition of the product of the gene by a drug should also be lethal. However, many effective drugs have been found to affect a handful of targets instead of a single protein. Recently, understanding of biological networks and molecule functionality has given rise to a new discipline, network pharmacology, that provides the opportunity to identify new drug targets in an infectious organism, while avoiding targets that cause toxicity in the host. Our group recently combined a genes essentiality and network properties to facilitate the identification of potential drug targets in treating Pseudomonas infection.. ...
I havent swore on this blog much, coz its a limited form of communication, isnt it? Yesterday I felt like it but it would have just turned out like the fucking fuckers fucked, it can get fucked for fucks sake or something. And all I really have is questions with no answers. And I feel like Ive been hit by a stealth CF infection with added wheeze factor. Last summer I was 10 stone 10, now Ive dropped down to 9 stone 12 which has completely mystified me; I have no idea how this happened. Can a stealth pseudomonas infection really rob me of so many calories? The doctor reckons it could be but I remain baffled...On the plus side my stomach is really flat ...
HIPS researchers produced a molecule that provides a way to visualise Pseudomonas infections weiter … Source:: http://idw-online.de/de/news686100. ...
1 Answer - Posted in: cancer, chronic, hospital, pseudomonas - Answer: Hi, my name is Liz, how did they treat the pseudomonis? What antibiotics ...
Semantic Scholar extracted view of Experimental Pseudomonas burn sepsis--evaluation of topical therapy. by Thomas J. Krizek et al.
Pseudomonas aeruginosa lung infections are a major cause of death in cystic fibrosis and hospitalized patients. Treating these infections is becoming difficult due to the emergence of conventional antimicrobial multiresistance. While monosaccharides have proved beneficial against such bacterial lung infection, the design of several multivalent glycosylated macromolecules has been shown to be also beneficial on biofilm dispersion. In this study, calix[4]arene-based glycoclusters functionalized with galactosides or fucosides have been synthesized. The characterization of their inhibitory properties on Pseudomonas aeruginosa aggregation, biofilm formation, adhesion on epithelial cells, and destruction of alveolar tissues were performed. The antiadhesive properties of the designed glycoclusters were demonstrated through several in vitro bioassays. An in vivo mouse model of lung infection provided an almost complete protection against Pseudomonas aeruginosa with the designed glycoclusters.. ...
Gilead is developing a broad spectrum combination antibiotic (FTI) consisting of fosfomycin (an antibiotic with activity against gram-positive and gram-negative bacteria) and tobramycin (an aminoglycoside antibiotic with potent gram-negative activity) for treatment of patients with CF. FTI offers a potential option for treatment of CF lung infections. It is important to note that the concentration of tobramycin in FTI is lower than that of the approved dose of inhaled tobramycin alone, thereby demonstrating the potential of FTI to minimize long-term toxicity from repeated exposure to aminoglycosides like tobramycin. This study will evaluate the safety and efficacy of 2 dose combinations of fosfomycin/tobramycin for inhalation (FTI), following a 28-day course of Aztreonam for Inhalation (AZLI) in patients with cystic fibrosis and Pseudomonas aeruginosa lung infection ...
Emond et al are reporting in Nat Genetics the identification of DCTN4 as a modifier for P.aeruginosa infection in patients with cystic fibrosis. It is a well-established fact that the majority of patients with cystic fibrosis develop acute and chronic P.aerugonisa infections which are associated with a worse clinical outcome. The authors selected and exome sequenced 91 patients from the EPIC collection with cystic fibrosis and P.aeruginosa and after performing logistic regression adjusted for ancestry and for CFTR mutation risk group identified DCTN4 as the only modifier gene. Dynactin 4 is a component of the dynein-dependent motor that moves autophagosomes along microtubules into lysosomes for degradation as part of the autophagy process which has an essential role in the clearance of P. aeruginosa. The presence of at least one DCTN4 missense variant was significantly associated with both early age of first P. aeruginosaâ€positive culture and with early age at onset of chronic P. aeruginosa ...
Clinical trial: Efficacy & Tolerability of Tobramycin Podhaler in Bronchiectasis Patients With Chronic Pseudomonas Aeruginosa Infection
Amaç: Bu çalışmada Psödomonas aeruginosa (P. aeruginosa) ile sıçan modelinde oluşturulan kronik akciğer infeksiyonlarının renal arterde ateroskleroz oluşumundaki rolü araştırıldı.. Çalışma planı: Altmış altı Wistar albino sıçan her grupta 14 ile 20 adet olacak şekilde randomize olarak dört gruba ayrıldı. Grup 1 (n=20) ve grup 3 (n=16) %1 kolesterol eklenmiş diyet ile, grup 2 (n=14) ve grup 4 (Kontrol grubu) standart sıçan yemi ile beslendi. Sıçanların trakeaları anestezi altında cerrahi olarak çıkarıldı. Çalışma periyodu esnasında dört haftalık aralıklarla toplam beş defa bir enjektörle grup 1 ve 2ye P. aeruginosa süspansiyonu ile grup 3 ve 4e ise serum fizyolojik ile intratrakeal inokülasyon yapıldı. Kolesterol seviyeleri anestezi altında kuyruktan alınan kan örneklerinden değerlendirildi.. Bulgular: Histopatolojik incelemelerde en fazla luminal incelme olan segment gözle inceleme yoluyla seçildi ve her segmentten 8-10 kesit ...
The opportunistic human pathogen Pseudomonas aeruginosa is the predominant micro-organism of chronic lung infections in cystic fibrosis patients. P. aeruginosa colonizes the lungs by forming biofilm microcolonies throughout the lung. Quorum sensing (QS) renders the biofilm bacteria highly tolerant t …
A total of 3,700 Pseudomonas aeruginosa isolates were collected from 17 general hospitals in Japan from 1992 to 1994. Of these isolates, 132 carbapenem-resistant strains were subjected to DNA hybridization analysis with the metallo-beta-lactamase gene (blaIMP)-specific probe. Fifteen strains carrying the metallo-beta-lactamase gene were identified in five hospitals in different geographical areas. Three strains of P. aeruginosa demonstrated high-level imipenem resistance (MIC, , or = 128 micrograms/ml), two strains exhibited low-level imipenem resistance (MIC, , or = 4 micrograms/ml), and the rest of the strains were in between. These results revealed that the acquisition of a metallo-beta-lactamase gene alone does not necessarily confer elevated resistance to carbapenems. In several strains, the metallo-beta-lactamase gene was carried by large plasmids, and carbapenem resistance was transferred from P. aeruginosa to Escherichia coli by electroporation in association with the acquisition of the ...
The early endobronchial inflammation induced by Pseudomonas aeruginosa infection varies in resistant and susceptible strains of mice. Mice of the DBA/2 strain are severely afflicted by the infection, with a high bacterial burden accumulating rapidly following inoculation and a high mortality rate occurring. Mice of the BALB/c strain are resistant to infection and clear the bacteria within 3 to 7 days. Infection of (BALB/c x DBA/2)F1 hybrid mice showed that the resistance to lung P. aeruginosa infection is inherited as a dominant trait. Mice of the A/J and C57BL/6 strains were found to have an intermediate phenotype to Pseudomonas aeruginosa infection when compared with BALB/c and DBA/2 strains. The decrease in the bacterial load seen early after infection coincided with a steady and strong recruitment of inflammatory cells to the bronchoalveolar spaces of mice of the resistant BALB/c strain. On the other hand, the recruitment of inflammatory cells to the lungs of mice of the susceptible DBA/2 ...
TY - JOUR. T1 - Dual β-lactam combination therapy for multi-drug resistant Pseudomonas aeruginosa infection. T2 - enhanced efficacy in vivo and comparison with monotherapies of penicillin-binding protein inhibition. AU - Siriyong, Thanyaluck. AU - Murray, Rachael M.. AU - Bidgood, Lucy E.. AU - Young, Simon A.. AU - Wright, Florence. AU - Parcell, Benjamin J.. AU - Voravuthikunchai, Supayang Piyawan. AU - Coote, Peter J.. PY - 2019/6/24. Y1 - 2019/6/24. N2 - The aim of the study was to determine the efficacy of dual β-lactam combination treatments derived from eight approved drugs against Galleria mellonella larvae infected with MDR strains of P. aeruginosa. Carbapenem-resistant P. aeruginosa NCTC 13437 and an unrelated clinical isolate were used to infect G. mellonella larvae and the efficacy of twenty-eight dual β-lactam combination therapies were compared to their constituent monotherapies. For the most potent combinations identified, penicillin-binding protein (PBP) inhibition profiles ...
A total of 183 patients were colonized or infected with multidrug-resistant Pseudomonas aeruginosa isolates at a hospital in Spain during 2007-2010; prevalence increased over this period from 2.8% to 15.3%. To characterize these isolates, we performed molecular epidemiologic and drug resistance analysis. Genotyping showed that 104 (56.8%) isolates belonged to a single major clone (clone B), which was identified by multilocus sequence typing as sequence type (ST) 175. This clone was initially isolated from 5 patients in 2008, and then isolated from 23 patients in 2009 and 76 patients in 2010. PCR analysis of clone B isolates identified the bla(VIM-2) gene in all but 1 isolate, which harbored bla(IMP-22). ST175 isolates were susceptible to only amikacin (75%) and colistin (100%). Emergence of the ST175 clone represents a major health problem because it compromises therapy for treatment of P. aeruginosa nosocomial infections ...
Background & Objective: The spread of carbapenem-resistant Pseudomonas aeruginosa is a global concern. Metallo-beta-lactamase (MBL) enzymes cause extensive drug resistance among Gram-negative bacteria. The current study aimed at determining the prevalence of MBL-producing P. aeruginosa in Iran. Methods: A total of 43 studies were found out of which 36 were adopted. Data were collected from Google, Google Scholar, Science Direct, PubMed, Scopus, Embase, and Sciverse. The terms
Resistant Pseudomonas Aeruginosa Infections Drugs Market Insights: Global Industry Analysis, Market Drivers, Restraints, Opportunities, Applications, Trends And Forecasts 2020-2026
Summary Pharmaceutical and Healthcare disease pipeline guide Resistant Pseudomonas aeruginosa Infections - Pipeline Review, H1 2017, provides an overview o
Video articles in JoVE about pseudomonas aeruginosa include Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice, Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants, A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection, Microtiter Dish Biofilm Formation Assay, Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response, Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays.
Twenty-three patients (85.2%) were infected with MDR P. aeruginosa, confirmed with cultures and resistance tests, during the ICU stay. In greater part, they were isolated from respiratory and urinary tract infections (33.3% and 25.9%, respectively). Four patients were treated empirically, with 50% of therapy response. The study group presented a mean age of 63 years, 51.9% males, with a mean Acute Physiology and Chronic Health Evaluation II score of 24.63. Sixty-three percent of our patients were first admitted to the hospital with community-acquired infection, none caused by P. aeruginosa. The most frequent cause for ICU admission was communitarian or nosocomial respiratory tract infection (29.9%). The mean time of polimixin B use was 15.59 days. Twelve patients (44%) used imipinem, 12 patients (44%) used teicoplanin and three patients (11%) used vancomicin, for more than 3 days, during the ICU stay before polimixin B use. After treatment with polimixin B, we had 40.7% response and improvement ...
Ozer B, Duran N, Onlen Y, Savas L. Efflux pump genes and antimicrobial resistance of Pseudomonas aeruginosa strains isolated from lower respiratory tract infections acquired in an intensive care unit. J Antibiot 2012; 65(1): 9-13. Badamchi, A., Masoumi, H., Javadinia, S., Asgarian, R. and Tabatabaie, A., 2017. Molecular detection of six virulence genes in Pseudomonas aeruginosa isolates detected in children with urinary tract infection. Microb Pathog 107: 44-47. Farra A, Islam S, Stralfors A, et al. Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem. Int J Antimicrob Agents 2008; 31(5): 427-33. Fazeli H, Havaei SA, Solgi H, et al. Pattern of antibiotic resistance in Pesudomonas aeruginosa isolated from intensive care unit, Isfahan, Iran. Journal of Isfahan Medical School 2013; 31(232): 432-438. Gutierrez O, Juan C, Cercenado E, et al. Molecular epidemiology and mechanisms of carbapenem resistance in Pseudomonas ...
Asuncion, Camille Carissa A. and Villadelrey, Michelle F. (2008) Synergistic effect of anti-pseudomonal agents and aminoglycosides on multi-drug resistant Pseudomonas aeruginosa. Undergraduate thesis, De La Salle University-Dasmariñas. ...
Cyclic-diguanylate (c-di-GMP) is a widespread bacterial signal molecule that plays a major role in the modulation of cellular surface components, such as exopolysaccharides and fimbriae, and in the establishment of a sessile life style. Here, we report that intracellular c-di-GMP levels influence cupA-encoded fimbriae expression in Pseudomonas aeruginosa. In an autoaggregative P. aeruginosa small colony variant (SCV) CupA fimbriae and the intracellular c-di-GMP concentration were found to be enhanced as compared with the clonal wild-type. The SCV morphology and the expression of CupA fimbriae were dependent on a functional PA1120 and morA gene both encoding a GGDEF domain. Overexpression of the GGDEF domain protein PA1120 complemented the PA1120 and the morA mutant with respect to CupA fimbriae expression. In agreement with these findings, overexpression of the EAL domain containing phenotypic variance regulator (PvrR) in the SCV resulted in a decreased intracellular level of c-di-GMP, a reduced ...
Phytopathology 102:575-587...Phytopathology 102:575-587...Effect of Overexpressing rsmA from Pseudomonas aeruginosa on Virulence of Select Phytotoxin-Producing Strains of P. syringae...Hye Suk Kong, Daniel P. Roberts, Cheryl D. Patterson, Sarah A. Kuehne, Stephan Heeb, Dilip K. Lakshman, and John Lydon...
Data of the study: Direct and indirect impact of the bacterial strain Pseudomonas aeruginosa on the dissolution of synthetic Fe(III)- and Fe(II)-bearing basaltic glasses
Many of the virulence factors produced by the opportunistic human pathogen Pseudomonas aeruginosa are quorum-sensing (QS) regulated. Among these are rhamnolipids, which have been shown to cause lysis of several cellular components of the human immune system, e.g. monocyte-derived macrophages and polymorphonuclear leukocytes (PMNs). We have previously shown that rhamnolipids produced by P. aeruginosa cause necrotic death of PMNs in vitro. This raises the possibility that rhamnolipids may function as a biofilm shieldin vivo, which contributes significantly to the increased tolerance of P. aeruginosa biofilms to PMNs. In the present study, we demonstrate the importance of the production of rhamnolipids in the establishment and persistence of P. aeruginosa infections, using an in vitro biofilm system, an intraperitoneal foreign-body model and a pulmonary model of P. aeruginosa infections in mice. Our experimental data showed that a P. aeruginosa strain, unable to produce any detectable ...
TY - JOUR. T1 - Detection of bacteriophage particles containing antibiotic resistance genes in the sputum of cystic fibrosis patients. AU - Brown-Jaque, Maryury. AU - Oyarzun, Lirain Rodriguez. AU - Cornejo-Sánchez, Thais. AU - Martín-Gómez, Maria T.. AU - Gartner, Silvia. AU - de Gracia, Javier. AU - Rovira, Sandra. AU - Alvarez, Antonio. AU - Jofre, Joan. AU - González-López, Juan J.. AU - Muniesa, Maite. PY - 2018/5/1. Y1 - 2018/5/1. N2 - © 2018 Brown-Jaque, Rodriguez Oyarzun, Cornejo-Sánchez, Martín-Gómez, Gartner, de Gracia, Rovira, Alvarez, Jofre, González-López and Muniesa. Cystic fibrosis (CF) is a chronic disease in which the bacterial colonization of the lung is linked to an excessive inflammatory response that leads to respiratory failure. The microbiology of CF is complex. Staphylococcus aureus is the first bacterium to colonize the lungs in 30% of pediatric CF patients, and 80% of adult patients develop a chronic Pseudomonas aeruginosa infection, but other microorganisms ...
Purpose of reviewRecent articles of clinical interest on Pseudomonas aeruginosa respiratory tract infections including CAP, nosocomially-acquired pneumonia, particularly in the ventilated patient, and chronic infections in cystic fibrosis patients are reviewed.Recent findingsThe growing importance o
TY - JOUR. T1 - Pseudomonas aeruginosa infection in cystic fibrosis. T2 - biosynthesis of alginate as a virulence factor.. AU - Roychoudhury, S.. AU - Zielinski, N. A.. AU - DeVault, J. D.. AU - Kato, J.. AU - Shinabarger, D. L.. AU - May, T. B.. AU - Maharaj, R.. AU - Kimbara, K.. AU - Misra, T. K.. AU - Chakrabarty, A. M.. PY - 1991/12/1. Y1 - 1991/12/1. UR - http://www.scopus.com/inward/record.url?scp=0026309286&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026309286&partnerID=8YFLogxK. M3 - Review article. C2 - 1801646. AN - SCOPUS:0026309286. VL - 44. SP - 63. EP - 67. JO - Antibiotics and chemotherapy. JF - Antibiotics and chemotherapy. SN - 0066-4758. ER - ...
Los mecanismos innatos y adquiridos de resistencia a los antibióticos en Pseudomonas representan un reto para los médicos que buscan una quimioterapia oportuna y eficaz. Esto es par- ticularmente importante en las áreas de cuidados intesnsivos de los hospitales. Este estudio está dirigido a lograr una comprensión a nivel molecular de dos de los más importantes mecanismos de resistencia a los fármacos en Pseudomonas aeruginosa. Cien aislados clínicos de Pseudomonas aeruginosa se obtuvieron de un hospital de tercer nivel en Quito, Ecuador. Se analizó la expresión de ampC y oprD mediante PCR cuantitativa en tiempo real. Se realizó una comparación entre los perfiles de expresión ampC y oprD y los fenotipos obtenidos en la prueba de susceptibilidad antimicrobiana (AST), con más del 50% de los aislados con perfiles concordantes para la expresión ampC y oprD. Nuestros resultados sugieren que la expresión ampC y oprD podría proporcionar información útil sobre mecanismos de resistencia ...
Pseudomonas aeruginosa Serotype 2B antibody LS-C538938 is an FITC-conjugated mouse monoclonal antibody to pseudomonas aeruginosa Pseudomonas aeruginosa Serotype 2B. Validated for ELISA.
http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1027-3948&volume=23&issue=2 Supp&spage=67&epage=&date=2001&atitle=Pseudomonas+aeruginosa+infection+is+associated+with+reduced+exhaled+nitric+oxide+(NO)+in+stable+ ...
Haji SH. Detection of Biofilm Formation in Pseudomonas aeruginosa Isolates from Clinical Specimens. Zanco J Pure Appl Sci. 2018; 30[4]:83-89. doi: https://doi.org/10.21271/ZJPAS.30.4.9 Saha S, Devi KM, Damrolien S, Devi KS, . K, Sharma KT. Biofilm production and its correlation with antibiotic resistance pattern among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital in north-east India. Int J Adv Med. 2018;5[4]:964. doi: http://dx.doi.org/10.18203/2349-3933.ijam20183129 Vallés J, Mariscal D, Cortés P, Coll P, Villagrá A, Díaz E, et al. Patterns of colonization by Pseudomonas aeruginosa in intubated patients: A 3-year prospective study of 1,607 isolates using pulsed-field gel electrophoresis with implications for prevention of ventilator-associated pneumonia. Intensive Care Med. 2004; 30[9]:1768-1775. doi: https://doi.org/10.1007/s00134-004-2382-6. Gales AC, Jones RN, Turnidge J, Rennie R, Ramphal R. Characterization of Pseudomonas aeruginosa Isolates: Occurrence Rates, ...
The CFTR-PTEN complex regulates cell metabolism and the inflammatory response to Pseudomonas aeruginosa infection. Lack of CFTR-PTEN function, as seen in cystic fibrosis, induces excessive secretion of metabolites succinate and itaconate. Pseudomonas aeruginosa exploits succinate and itaconate to produce pulmonary infection. ...
Pseudomonas aeruginosa is an ubiquitous organism. Its ability to survive on minimal nutritional requirements and to tolerate a variety of physical conditions allows its persistence in both community and hospital settings [12]. P. aeruginosa is a serious therapeutic challenge for treatment of both community-acquired and nosocomial infections, due to the ability of this microorganism to develop resistance to multiple classes of antibacterial agents, even during the course of therapy [13, 14]. The increasing frequency of MDR or XDR P. aeruginosa strains is of concern as effective antimicrobial options are limited [15, 16]. Moreover, only a few new antibiotics are currently under development [6]. An increase in MDR bacterial infections among companion animals has been documented in multiple veterinary hospital settings [17]. This is of particular importance due to the risk of transmission to humans and other companion animals in close contact with infected animals, even because in our countries the ...
Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide Academic Article ...
Annotation. Over the last ten years, there has been an increase in the incidence of bacterial keratitis caused by Pseudomonas aeruginosa, associated with contact lenses use. Certain features of the course of such keratitis, the mechanisms of corneal damage, the factors that affect the severity and depth of tissue destruction remain unclear. Therefore, the aim of our work was to model experimental pseudomonal keratitis in rabbits using different methods of infection and to investigate the microbiological and clinical aspects of keratitis depending on the method of infection. As a result of the study, we were able to reproduce superficial and deep keratitis with different methods of infection. Our proposed method cornea infection with bacterial films on a contact lens allowed to reproduce severe keratitis with a longer release of the pathogen from the surface of the affected cornea. It was also found that the results of microbiological examination of the material from the affected eye do not ...
Anti Pseudomonas aeruginosa Serotype 8 Antibody, clone 1017/87 , Mouse Anti-Bacterial Monoclonal Antibody validated in WB (ABD12646), Abgent
Prime Journal of Microbiology Research (PJMR) ISSN: 2251-1261. susceptible and P. aeruginosa the least. Antibiotic use is suggested to be a major risk factor for.. chromID ™ P.aeruginosa Chromogenic medium for direct ID of Pseudomonas aeruginosa. Deliver rapid direct identification of Pseudomonas aeruginosa to contribute to.chromID™ P.aeruginosa Chromogenic medium for direct ID of Pseudomonas aeruginosa. Deliver rapid direct identification of Pseudomonas aeruginosa to contribute to.RESEARCH ARTICLE Open Access Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections Youenn Alexandre1, Rozenn Le Berre1,2*.tetracycline, Tetracycline is an antibiotic used to treat a number of bacterial infections. It is commonly used to treat acne and rosacea. Historically it was.Original article Antibiotic resistance and virulence properties of Pseudomonas aeruginosa strains from mechanically ventilated patients with pneumonia in intensive ...
RESULTS: As a result of the antibiotic resistance analysis, 11 different antibiotypes for Hospital 1 and Hospital 3, while 6 different antibiotypes for Hospital 2 were determined. The highest incidence of MDR strains was observed in Hospital 1. It was found that MDR strains were frequently isolated from patients who underwent surgical interventions or patients in the intensive care units. As a result of genotyping, 9 different genotypes were determined in Hospital 1, 7 in Hospital 2, and 17 in Hospital 3. There was no significant correlation between the antibiotypes and genotypes. A clonal relationship between the MDR strains that were isolated from both the same service and from different services was observed in Hospital 1. These findings support the hypothesis that the strains are spread within the hospital via cross or horizontal transmission through hospital employees or medical instruments. According to Simpsons diversity index, the RAPD-PCR methods discrimination power was found as 0.92 ...
TY - JOUR. T1 - Slime production a virulence marker in Pseudomonas aeruginosa strains isolated from clinical and environmental specimens. T2 - A comparative study of two methods. AU - Prasad, S. Vishnu. AU - Ballal, Mamatha. AU - Shivananda, P. G.. PY - 2009/4/1. Y1 - 2009/4/1. N2 - Detection of slime in Pseudomonas aeruginosa can be useful in understanding the virulence of this organism. Here, comparative studies of two phenotypic methods using the tube method and the spectrophotometric method for slime production from 100 clinically and 21 environmentally significant isolates of P. aeruginosa were performed. A total of 68 isolates were positive by either of the tests whereas only 34 were positive by both the tests. The tube method detected slime significantly in more number of isolates than the spectrophotometric method. The tube test was found to be superior to the spectrophotometric method in ease of performance, interpretation and sensitivity. Among the clinical isolates, systemic isolates ...
BioAssay record AID 164894 submitted by ChEMBL: In vitro antibacterial activity against AZT resistant Pseudomonas aeruginosa 69 was determined.
BioAssay record AID 164900 submitted by ChEMBL: In vitro antibacterial activity against IPM resistant Pseudomonas aeruginosa 56 was determined.
An organism of concern, Pseudomonas aeruginosa is a water-loving bacteria that works and builds biofilms with other threatening bacteria. Understanding that disinfection alone will not rid an engineered water system of bacteria means we need to look toward biofilm-resistant material and disinfection at the source of use as opposed to where the water enters the building.. Antibiotic Resistance of Pseudomonas aeruginosa in Pneumonia at a Single University Hospital Center in Germany over a 10-Year Perioddetermines that while P. aeruginosa and MDR P. aeruginosa were resistant to a variety of commonly used antibiotics, they were not resistant to colistin in the few isolates recovered from patients with pneumonia.. Inhibition of Aspergillus fumigatus and Its Biofilm by Pseudomonas aeruginosa Is Dependent on the Source, Phenotype and Growth Conditions of the Bacterium reports that Aspergillus fumigatus (Af) and Pseudomonas aeruginosa (Pa) are leading fungal and bacterial pathogens, respectively, in ...
Plasma ferritin is an important extracellular iron storage molecule, whose concentration increases drastically in cancer and infection. During infection, the pathogen usurps host iron for its survival and pathogenicity; hence, maintenance of the plasma ferritin level during infection is a crucial host defence mechanism. In this study, the horseshoe crab plasma ferritin complex was purified, characterized, and its involvement in innate immune defence was investigated. The plasma ferritin appears as a 21-kDa subunit on SDS-PAGE. Full-length ferritin-H cDNAs (CrFer-H1 and CrFer-H2) were cloned. Analysis of the 5′ UTR indicates the existence of a functional iron-response element, suggesting that both the CrFer-H genes may be post-transcriptionally regulated. Northern analysis shows that the CrFer-H is ubiquitously expressed. Within 3 h of lipopolysaccharide challenge, the gene is up-regulated by > 12-fold. In contrast, iron-loading did not result in any significant change. When challenged with ...
Author Summary Pathogens face a hostile and often novel environment when infecting a new host, and adaptation to this environment can be critical to a pathogens survival. The genetic basis of pathogen adaptation is in turn important for treatment, since the consistency with which therapies succeed may depend on the extent to which a pathogen adapts via the same routes in different patients. In this study, we investigate adaptation of the bacterium Pseudomonas aeruginosa to laboratory conditions that resemble the lungs of cystic fibrosis patients and to quinolone antibiotics. We find that a handful of genes and genetic pathways are repeatedly involved in adaptation to each condition. Nonetheless, other, less common mutations can play important roles in determining fitness, complicating strategies aimed at reducing the prevalence of antibiotic resistance.
Author Summary Pathogens face a hostile and often novel environment when infecting a new host, and adaptation to this environment can be critical to a pathogens survival. The genetic basis of pathogen adaptation is in turn important for treatment, since the consistency with which therapies succeed may depend on the extent to which a pathogen adapts via the same routes in different patients. In this study, we investigate adaptation of the bacterium Pseudomonas aeruginosa to laboratory conditions that resemble the lungs of cystic fibrosis patients and to quinolone antibiotics. We find that a handful of genes and genetic pathways are repeatedly involved in adaptation to each condition. Nonetheless, other, less common mutations can play important roles in determining fitness, complicating strategies aimed at reducing the prevalence of antibiotic resistance.
Resistance to extended-spectrum cephalosporins complicates treatment of Pseudomonas aeruginosa infections. To elucidate risk factors for cefepime-resistant P. aeruginosa and determine its association with patient death, we conducted a case-control study in Philadelphia, Pennsylvania. Among 2,529 patients hospitalized during 2001-2006, a total of 213 (8.4%) had cefepime-resistant P. aeruginosa infection. Independent risk factors were prior use of an extended-spectrum cephalosphorin (p<0.001), prior use of an extended-spectrum penicillin (p = 0.005), prior use of a quinolone (p<0.001), and transfer from an outside facility (p = 0.01). Among those hospitalized at least 30 days, mortality rates were higher for those with cefepime-resistant than with cefepime-susceptible P. aeruginosa infection (20.2% vs. 13.2%, p = 0.007). Cefepime-resistant P. aeruginosa was an independent risk factor for death only for patients for whom it could be isolated from blood (p = 0.001). Strategies to counter its
Multidrug resistant (MDR) Pseudomonas aeruginosa has caused serious nosocomial infections owing to its high intrinsic resistance and ease of acquiring resistance to common antibiotics. There is an urgent need to develop antimicrobial agents against MDR Pseudomonas aeruginosa. Here we report a 27-mer peptide polymer
Pseudomonas aeruginosa is an important opportunistic pathogen usually resistant to most antimicrobials. We present changes in the resistance pattern of R aeruginosa to amikacin (AK) and ciprofloxacin (CIP) between January 2002 and June 2004. The physicians of each unit were given information on antibiotic resistance rates of P aeruginosa isolated from ward patients at regular intervals. The antibiotic resistance of 161 P aeruginosa isolates isolated from intensive care units (ICUs) and non-ICUs were tested by disk diffusion method, and the results were interpreted according to the guidelines of National Committee for Clinical Laboratory Standards. Thirty-five percent of all the P aeruginosa isolates were resistant to AK in 2002, 18% in 2003, and 20% in 2004. The CIP resistance rates were 4% in 2002, 26% in 2003 and 20% in 2004. In that period, resistance to AK decreased, whereas resistance to CIP increased. The usage rate of AK in 2002 was 32%, which fell to 26% in 2003 ( ...
Wright, Laura, Turton, Jane F., Hopkins, Katie L, Livermore, David and Woodford, Neil (2015) Genetic environment of metallo-β-lactamase genes in Pseudomonas aeruginosa isolates from the UK. Journal of Antimicrobial Chemotherapy, 70 (12). pp. 3250-3258. ISSN 0305-7453 ...
Looking for Pseudomonas aeruginosa? Find out information about Pseudomonas aeruginosa. An opportunistic pathogen that is the most significant cause of hospital-acquired infections, particularly in predisposed patients with metabolic,... Explanation of Pseudomonas aeruginosa