BioAssay record AID 164064 submitted by ChEMBL: Antibacterial activity against gram-negative bacteria Pseudomonas aeruginosa ATCC 27853; No effect.
Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide Academic Article ...
Cystic fibrosis (CF) lung disease begins in the first few months of life and follows a course of recurrent lower airway bacterial infection and inflammation and progression of disease over years and decades at a variable pace. With the development of chronic lung infection, obstructive disease progressively worsens, ultimately leading to respiratory failure. Pseudomonas aeruginosa (Pa) is the most important pathogen infecting the CF lower airways, and its acquisition early in life is associated with a pro-inflammatory effect, lower lung function, poor nutritional outcomes, and decreased survival.. Pseudomonas aeruginosa (Pa) infection of the cystic fibrosis (CF) airway typically proceeds from early infection to chronic infection. Although some studies have shown that a minority of individuals with CF spontaneously clear early Pseudomonas aeruginosa (Pa) infection, data from multiple studies suggest that antibiotics are superior to no treatment in clearing Pseudomonas aeruginosa (Pa) from ...
Many of the virulence factors produced by the opportunistic human pathogen Pseudomonas aeruginosa are quorum-sensing (QS) regulated. Among these are rhamnolipids, which have been shown to cause lysis of several cellular components of the human immune system, e.g. monocyte-derived macrophages and polymorphonuclear leukocytes (PMNs). We have previously shown that rhamnolipids produced by P. aeruginosa cause necrotic death of PMNs in vitro. This raises the possibility that rhamnolipids may function as a biofilm shieldin vivo, which contributes significantly to the increased tolerance of P. aeruginosa biofilms to PMNs. In the present study, we demonstrate the importance of the production of rhamnolipids in the establishment and persistence of P. aeruginosa infections, using an in vitro biofilm system, an intraperitoneal foreign-body model and a pulmonary model of P. aeruginosa infections in mice. Our experimental data showed that a P. aeruginosa strain, unable to produce any detectable ...
Pseudomonas aeruginosa lung infections are a major cause of death in cystic fibrosis and hospitalized patients. Treating these infections is becoming difficult due to the emergence of conventional antimicrobial multiresistance. While monosaccharides have proved beneficial against such bacterial lung infection, the design of several multivalent glycosylated macromolecules has been shown to be also beneficial on biofilm dispersion. In this study, calix[4]arene-based glycoclusters functionalized with galactosides or fucosides have been synthesized. The characterization of their inhibitory properties on Pseudomonas aeruginosa aggregation, biofilm formation, adhesion on epithelial cells, and destruction of alveolar tissues were performed. The antiadhesive properties of the designed glycoclusters were demonstrated through several in vitro bioassays. An in vivo mouse model of lung infection provided an almost complete protection against Pseudomonas aeruginosa with the designed glycoclusters.. ...
The opportunistic human pathogen Pseudomonas aeruginosa is the predominant micro-organism of chronic lung infections in cystic fibrosis patients. P. aeruginosa colonizes the lungs by forming biofilm microcolonies throughout the lung. Quorum sensing (QS) renders the biofilm bacteria highly tolerant to otherwise lethal doses of antibiotics, and protects against the bactericidal activity of polymorphonuclear leukocytes (PMNs). It has been previously demonstrated that QS is inhibited by garlic extract. In this study, the synergistic effects of garlic and tobramycin, and PMNs activities have been evaluated. P. aeruginosa was grown in vitro in continuous-culture once-through flow chambers with and without garlic extract. The garlic-treated biofilms were susceptible to both tobramycin and PMN grazing. Furthermore, the PMNs showed an increase in respiratory burst activation, when incubated with the garlic-treated biofilm. Garlic extract was administered as treatment for a mouse pulmonary infection model. Mice
Bacterial resistance to conventional antibiotics combined with an increasing acknowledgement of the role of biofilms in chronic infections has led to a growing interest in new antimicrobial strategies that target the biofilm mode of growth. In the aggregated biofilm mode, cell-to-cell communication systems involved in the process known as quorum sensing regulate coordinated expression of virulence with immune shielding mechanisms and antibiotic resistance. For two decades, the potential of interference with quorum sensing by small chemical compounds has been investigated with the aim of developing alternative antibacterial strategies. Here, we review state of the art research of quorum sensing inhibitors against the opportunistic human pathogen Pseudomonas aeruginosa, which is found in a number of biofilm-associated infections and identified as the predominant organism infecting the lungs of cystic fibrosis patients ...
A total of 3,700 Pseudomonas aeruginosa isolates were collected from 17 general hospitals in Japan from 1992 to 1994. Of these isolates, 132 carbapenem-resistant strains were subjected to DNA hybridization analysis with the metallo-beta-lactamase gene (blaIMP)-specific probe. Fifteen strains carrying the metallo-beta-lactamase gene were identified in five hospitals in different geographical areas. Three strains of P. aeruginosa demonstrated high-level imipenem resistance (MIC, , or = 128 micrograms/ml), two strains exhibited low-level imipenem resistance (MIC, , or = 4 micrograms/ml), and the rest of the strains were in between. These results revealed that the acquisition of a metallo-beta-lactamase gene alone does not necessarily confer elevated resistance to carbapenems. In several strains, the metallo-beta-lactamase gene was carried by large plasmids, and carbapenem resistance was transferred from P. aeruginosa to Escherichia coli by electroporation in association with the acquisition of the ...
Bacteria synchronize group behaviors using quorum sensing, which is advantageous during an infection to thwart immune cell attack and resist deleterious changes in the environment. In Pseudomonas aeruginosa, the Pseudomonas quinolone signal (Pqs) quorum-sensing system is an important component of an interconnected intercellular communication network. Two alkylquinolones, 2-heptyl-4-quinolone (HHQ) and 2-heptyl-3-hydroxy-4-quinolone (PQS), activate transcriptional regulator PqsR to promote the production of quinolone signals and virulence factors. Our work focused on the most abundant quinolone produced from the Pqs system, 2,4-dihydroxyquinoline (DHQ), which was shown previously to sustain pyocyanin production and antifungal activity of P. aeruginosa. However, little is known about how DHQ affects P. aeruginosa pathogenicity. Using C. elegans as a model for P. aeruginosa infection, we found pqs mutants only able to produce DHQ maintained virulence towards the nematodes similar to wild-type. In addition,
Haji SH. Detection of Biofilm Formation in Pseudomonas aeruginosa Isolates from Clinical Specimens. Zanco J Pure Appl Sci. 2018; 30[4]:83-89. doi: https://doi.org/10.21271/ZJPAS.30.4.9 Saha S, Devi KM, Damrolien S, Devi KS, . K, Sharma KT. Biofilm production and its correlation with antibiotic resistance pattern among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital in north-east India. Int J Adv Med. 2018;5[4]:964. doi: http://dx.doi.org/10.18203/2349-3933.ijam20183129 Vallés J, Mariscal D, Cortés P, Coll P, Villagrá A, Díaz E, et al. Patterns of colonization by Pseudomonas aeruginosa in intubated patients: A 3-year prospective study of 1,607 isolates using pulsed-field gel electrophoresis with implications for prevention of ventilator-associated pneumonia. Intensive Care Med. 2004; 30[9]:1768-1775. doi: https://doi.org/10.1007/s00134-004-2382-6. Gales AC, Jones RN, Turnidge J, Rennie R, Ramphal R. Characterization of Pseudomonas aeruginosa Isolates: Occurrence Rates, ...
The success of Pseudomonas aeruginosa in cystic fibrosis (CF) and other chronic infections is largely attributed to its ability to grow in antibiotic-resistant biofilm communities. This study investigated the effects of limiting iron levels as a strategy for preventing/disrupting P. aeruginosa biofilms. A range of synthetic and naturally occurring iron-chelating agents were examined. Biofilm development by P. aeruginosa strain PAO1 and CF sputum isolates from chronically infected individuals was significantly decreased by iron removal under aerobic atmospheres. CF strains formed poor biofilms under anaerobic conditions. Strain PAO1 was also tested under anaerobic conditions. Biofilm formation by this model strain was almost totally prevented by several of the chelators tested. The ability of synthetic chelators to impair biofilm formation could be reversed by iron addition to cultures, providing evidence that these effective chelating compounds functioned by directly reducing availability of iron to P.
An organism of concern, Pseudomonas aeruginosa is a water-loving bacteria that works and builds biofilms with other threatening bacteria. Understanding that disinfection alone will not rid an engineered water system of bacteria means we need to look toward biofilm-resistant material and disinfection at the source of use as opposed to where the water enters the building.. Antibiotic Resistance of Pseudomonas aeruginosa in Pneumonia at a Single University Hospital Center in Germany over a 10-Year Perioddetermines that "while P. aeruginosa and MDR P. aeruginosa were resistant to a variety of commonly used antibiotics, they were not resistant to colistin in the few isolates recovered from patients with pneumonia.". Inhibition of Aspergillus fumigatus and Its Biofilm by Pseudomonas aeruginosa Is Dependent on the Source, Phenotype and Growth Conditions of the Bacterium reports that "Aspergillus fumigatus (Af) and Pseudomonas aeruginosa (Pa) are leading fungal and bacterial pathogens, respectively, in ...
Cystic fibrosis lung disease is characterized by chronic airway infections with the opportunistic pathogen Pseudomonas aeruginosa and severe neutrophilic pulmonary inflammation. P. aeruginosa undergoes extensive genetic adaptation to the cystic fibrosis (CF) lung environment, and adaptive mutations in the quorum sensing regulator gene lasR commonly arise. We sought to define how mutations in lasR alter host-pathogen relationships. We demonstrate that lasR mutants induce exaggerated host inflammatory responses in respiratory epithelial cells, with increased accumulation of proinflammatory cytokines and neutrophil recruitment due to the loss of bacterial protease-dependent cytokine degradation. In subacute pulmonary infections, lasR mutant-infected mice show greater neutrophilic inflammation and immunopathology compared with wild-type infections. Finally, we observed that CF patients infected with lasR mutants have increased plasma interleukin-8 (IL-8), a marker of inflammation. These findings ...
Prime Journal of Microbiology Research (PJMR) ISSN: 2251-1261. susceptible and P. aeruginosa the least. Antibiotic use is suggested to be a major risk factor for.. chromID ™ P.aeruginosa Chromogenic medium for direct ID of Pseudomonas aeruginosa. Deliver rapid direct identification of Pseudomonas aeruginosa to contribute to.chromID™ P.aeruginosa Chromogenic medium for direct ID of Pseudomonas aeruginosa. Deliver rapid direct identification of Pseudomonas aeruginosa to contribute to.RESEARCH ARTICLE Open Access Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections Youenn Alexandre1, Rozenn Le Berre1,2*.tetracycline, Tetracycline is an antibiotic used to treat a number of bacterial infections. It is commonly used to treat acne and rosacea. Historically it was.Original article Antibiotic resistance and virulence properties of Pseudomonas aeruginosa strains from mechanically ventilated patients with pneumonia in intensive ...
BioAssay record AID 478497 submitted by ChEMBL: Antibacterial activity against Pseudomonas aeruginosa at 2 mg/ml after 24 hrs by agar diffusion method.
Increasing rates of antibiotic resistance among Gram-negative pathogens such as Pseudomonas aeruginosa means alternative approaches to antibiotic development are urgently required. Pyocins, produced by P. aeruginosa for intraspecies competition, are highly potent protein antibiotics known to actively translocate across the outer membrane of P. aeruginosa. Understanding and exploiting the mechanisms by which pyocins target, penetrate and kill P. aeruginosa is a promising approach to antibiotic development. In this work we show the therapeutic potential of a newly identified tRNase pyocin, pyocin SD2, by demonstrating its activity in vivo in a murine model of P. aeruginosa lung infection. In addition, we propose a mechanism of cell targeting and translocation for pyocin SD2 across the P. aeruginosa outer membrane. Pyocin SD2 is concentrated at the cell surface, via binding to the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide (LPS), from where it can efficiently locate its outer
Increasing rates of antibiotic resistance among Gram-negative pathogens such as Pseudomonas aeruginosa means alternative approaches to antibiotic development are urgently required. Pyocins, produced by P. aeruginosa for intraspecies competition, are highly potent protein antibiotics known to actively translocate across the outer membrane of P. aeruginosa. Understanding and exploiting the mechanisms by which pyocins target, penetrate and kill P. aeruginosa is a promising approach to antibiotic development. In this work we show the therapeutic potential of a newly identified tRNase pyocin, pyocin SD2, by demonstrating its activity in vivo in a murine model of P. aeruginosa lung infection. In addition, we propose a mechanism of cell targeting and translocation for pyocin SD2 across the P. aeruginosa outer membrane. Pyocin SD2 is concentrated at the cell surface, via binding to the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide (LPS), from where it can efficiently locate its ...
Pseudomonas aeruginosa ATCC ® 47085D-5™ Designation: Genomic DNA from Pseudomonas aeruginosa strain PAO1-LAC TypeStrain=False Application:
Cluster II che Genes from Pseudomonas aeruginosa Are Required for an Optimal Chemotactic Response: Pseudomonas aeruginosa, a γ-proteobacterium, is motile by mea
Bacterial viruses, or phage, are key members of natural microbial communities. Yet much research on bacterial-phage interactions has been conducted in liquid cultures involving single bacterial strains. Here we explored how bacterial diversity affects the success of lytic phage in structured communities. We infected a sensitive Pseudomonas aeruginosa strain PAO1 with a lytic phage Pseudomonas 352 in the presence versus absence of an insensitive P. aeruginosa strain PA14, in liquid culture versus colonies on agar. We found that both in liquid and in colonies, inter-strain competition reduced resistance evolution in the susceptible strain and decreased phage population size. However, while all sensitive bacteria died in liquid, bacteria in colonies could remain sensitive yet escape phage infection, due mainly to reduced growth in colony centers. In sum, spatial structure can protect bacteria against phage infection, while the presence of competing strains reduces the evolution of resista
Pseudomonas aeruginosa Serotype 2B antibody LS-C538938 is an FITC-conjugated mouse monoclonal antibody to pseudomonas aeruginosa Pseudomonas aeruginosa Serotype 2B. Validated for ELISA.
A Pf1-like phage is involved in P. aeruginosa biofilm killing.Electron microscopic examination of the CsCl-purified phage revealed filamentous phage particles that were approximately 1.5 μm long (Fig. 3b). The genome of P. aeruginosa contains a filamentous prophage that is closely related to phage Pf1, and Pf1 genes are known to be upregulated in P. aeruginosa biofilms (66). Moreover, it is known that Pf1 can infect a cell by using T4P (24). Flagella have also been reported to be receptors for filamentous phage (44), and our data suggest that the P. aeruginosa Pf1-like phage may additionally infect a cell through the flagellum. We also carried out PCR with Pf1-specific primers 437F and 437R using DNA extracted from the CsCl-purified phage band. The 894-bp PCR product was sequenced, and the sequence showed 100% identity with the sequence of the Pf1-like prophage from P. aeruginosa. We also hybridized a PCR-labeled, Pf1-specific DNA probe with individual plaques generated from the biofilm ...
A Pf1-like phage is involved in P. aeruginosa biofilm killing.Electron microscopic examination of the CsCl-purified phage revealed filamentous phage particles that were approximately 1.5 μm long (Fig. 3b). The genome of P. aeruginosa contains a filamentous prophage that is closely related to phage Pf1, and Pf1 genes are known to be upregulated in P. aeruginosa biofilms (66). Moreover, it is known that Pf1 can infect a cell by using T4P (24). Flagella have also been reported to be receptors for filamentous phage (44), and our data suggest that the P. aeruginosa Pf1-like phage may additionally infect a cell through the flagellum. We also carried out PCR with Pf1-specific primers 437F and 437R using DNA extracted from the CsCl-purified phage band. The 894-bp PCR product was sequenced, and the sequence showed 100% identity with the sequence of the Pf1-like prophage from P. aeruginosa. We also hybridized a PCR-labeled, Pf1-specific DNA probe with individual plaques generated from the biofilm ...
Looking for Pseudomonas aeruginosa? Find out information about Pseudomonas aeruginosa. An opportunistic pathogen that is the most significant cause of hospital-acquired infections, particularly in predisposed patients with metabolic,... Explanation of Pseudomonas aeruginosa
ABSTRACT. The present work attempts to solve pollution problems in watery surroundings by aromatic compounds such as the phenol and the benzoic acid. Several ways of elimination of these compounds were the object of different research among which is the use of bacteria. In this framework, Pseudomonas aeruginosa bacterium is used to eliminate phenol and the benzoic acid. This made it possible to isolate the Pseudomonas aeruginosa bacterium directly on the nourishing environment containing phenol and benzoic acid as source of energy then the bacteria is incubated at 37˚C during a minimal duration of four days. Furthermore, we studied the influence of the Pseudomonas aeruginosa bacterium on the deterioration of an area exposed to a phenol and the benzoic acid concentration. Results obtained at the time of the different experimentations clearly show that phenol and the benzoic acid were eliminated by the Pseudomonas aeruginosa bacterium. However, it was noted that during the various investigations ...
TY - JOUR. T1 - Slime production a virulence marker in Pseudomonas aeruginosa strains isolated from clinical and environmental specimens. T2 - A comparative study of two methods. AU - Prasad, S. Vishnu. AU - Ballal, Mamatha. AU - Shivananda, P. G.. PY - 2009/4/1. Y1 - 2009/4/1. N2 - Detection of slime in Pseudomonas aeruginosa can be useful in understanding the virulence of this organism. Here, comparative studies of two phenotypic methods using the tube method and the spectrophotometric method for slime production from 100 clinically and 21 environmentally significant isolates of P. aeruginosa were performed. A total of 68 isolates were positive by either of the tests whereas only 34 were positive by both the tests. The tube method detected slime significantly in more number of isolates than the spectrophotometric method. The tube test was found to be superior to the spectrophotometric method in ease of performance, interpretation and sensitivity. Among the clinical isolates, systemic isolates ...
Background: Pseudomonas aeruginosa is one of the primary pathogens isolated more frequently in cystic fibrosis (CF) and it exhibits innate resistance to a wide range of antibiotics. Purpose: We sought to determine whether the highly prevalent genotypes of P. aeruginosa are specifically linked to CF patients and have any related multidrug antibiotic resistance. Isolates from hospitalized non-CF patients and from environmental sources were also genotypically analyzed. Methods: Collections of P. aeruginosa from lower respiratory secretions (n=45) were genotyped using pulsed-field gel electrophoresis (PFGE). Phenotypic screening for antibiotic susceptibility was performed for the common antimicrobial agents by E-test and automated Phoenix method. Results: P. aeruginosa isolates from CF (n=32), hospitalized non-CF patients (n=13), and environment sources (n=5) were analyzed. The population structure of P. aeruginosa is highly diverse and population-specific. All PFGE results of P. aeruginosa isolates ...
A total of 183 patients were colonized or infected with multidrug-resistant Pseudomonas aeruginosa isolates at a hospital in Spain during 2007-2010; prevalence increased over this period from 2.8% to 15.3%. To characterize these isolates, we performed molecular epidemiologic and drug resistance analysis. Genotyping showed that 104 (56.8%) isolates belonged to a single major clone (clone B), which was identified by multilocus sequence typing as sequence type (ST) 175. This clone was initially isolated from 5 patients in 2008, and then isolated from 23 patients in 2009 and 76 patients in 2010. PCR analysis of clone B isolates identified the bla(VIM-2) gene in all but 1 isolate, which harbored bla(IMP-22). ST175 isolates were susceptible to only amikacin (75%) and colistin (100%). Emergence of the ST175 clone represents a major health problem because it compromises therapy for treatment of P. aeruginosa nosocomial infections ...
Los mecanismos innatos y adquiridos de resistencia a los antibióticos en Pseudomonas representan un reto para los médicos que buscan una quimioterapia oportuna y eficaz. Esto es par- ticularmente importante en las áreas de cuidados intesnsivos de los hospitales. Este estudio está dirigido a lograr una comprensión a nivel molecular de dos de los más importantes mecanismos de resistencia a los fármacos en Pseudomonas aeruginosa. Cien aislados clínicos de Pseudomonas aeruginosa se obtuvieron de un hospital de tercer nivel en Quito, Ecuador. Se analizó la expresión de ampC y oprD mediante PCR cuantitativa en tiempo real. Se realizó una comparación entre los perfiles de expresión ampC y oprD y los fenotipos obtenidos en la prueba de susceptibilidad antimicrobiana (AST), con más del 50% de los aislados con perfiles concordantes para la expresión ampC y oprD. Nuestros resultados sugieren que la expresión ampC y oprD podría proporcionar información útil sobre mecanismos de resistencia ...
Pseudomonas aeruginosa causes aggressive infection in patients with pre-existing disorders and recurrent pulmonary infections in cystic fibrosis patients. Pathogenesis of P. aeruginosa infections is multifactorial owing to numerous virulence factors. The focus of this thesis research was to investigate whether P. aeruginosa elastase (PE) causes remodeling of the cytoskeleton by increasing the phosphorylation of RhoA GTPase proteins. In addressing our hypothesis, we utilized Small GTPase Immuno-sorbent Activation assays (G-LISA) and Enzyme linked Immuno-sorbent assay (ELISA) to quantitate changes in the total as well as phosphorylated RhoA protein in Calu3 cell lines. Fluorescence microscopy aided in understanding the changes in morphological organization of F-actin. Changes in expression of TJ protein, ZO1, due to PE induced RhoA GTPase activity, was analyzed with SDS PAGE and Western Blot Analysis. Our data from G-LISA and ELISA assays indicate that PE increases the amount of active RhoA protein by 50
Emond et al are reporting in Nat Genetics the identification of DCTN4 as a modifier for P.aeruginosa infection in patients with cystic fibrosis. It is a well-established fact that the majority of patients with cystic fibrosis develop acute and chronic P.aerugonisa infections which are associated with a worse clinical outcome. The authors selected and exome sequenced 91 patients from the EPIC collection with cystic fibrosis and P.aeruginosa and after performing logistic regression adjusted for ancestry and for CFTR mutation risk group identified DCTN4 as the only modifier gene. Dynactin 4 is a component of the dynein-dependent motor that moves autophagosomes along microtubules into lysosomes for degradation as part of the autophagy process which has an essential role in the clearance of P. aeruginosa. The presence of at least one DCTN4 missense variant was significantly associated with both early age of first P. aeruginosaâ€"positive culture and with early age at onset of chronic P. aeruginosa ...
The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.
Author Summary Pathogens face a hostile and often novel environment when infecting a new host, and adaptation to this environment can be critical to a pathogens survival. The genetic basis of pathogen adaptation is in turn important for treatment, since the consistency with which therapies succeed may depend on the extent to which a pathogen adapts via the same routes in different patients. In this study, we investigate adaptation of the bacterium Pseudomonas aeruginosa to laboratory conditions that resemble the lungs of cystic fibrosis patients and to quinolone antibiotics. We find that a handful of genes and genetic pathways are repeatedly involved in adaptation to each condition. Nonetheless, other, less common mutations can play important roles in determining fitness, complicating strategies aimed at reducing the prevalence of antibiotic resistance.
Understanding both the mechanistic basis of virulence and the evolutionary processes under which it can arise, is fundamental if we are to increase our knowledge of disease causing bacteria in an era of ever increasing antibiotic resistance. To date, there has been a substantial effort to understand virulence evolution both theoretically and experimentally. However, comparatively little experimental work has focussed on the evolution of virulence in opportunistic pathogens, and how virulence varies across multiple host organisms. In this thesis, the opportunist Pseudomonas aeruginosa was used to study virulence and its evolution in hosts. The virulence levels of different strains of P. aeruginosa, both laboratory and clinical, were tested in the nematode Caenorhabditis elegans. It was found that virulence of P. aeruginosa was lower in clinical strains isolated from chronic infections, and laboratory strains initially isolated from chronic infections, while a strain isolated from an acute ...
Quorum sensing, a cell-to-cell communication system based on small signal molecules, is employed by the human pathogen Pseudomonas aeruginosa to regulate virulence and biofilm development. Moreover, regulation by small trans-encoded RNAs has become a focal issue in studies of virulence gene expression of bacterial pathogens. In this study, we have identified the small RNA PhrS as an activator of PqsR synthesis, one of the key quorum-sensing regulators in P. aeruginosa. Genetic studies revealed a novel mode of regulation by a sRNA, whereby PhrS uses a base-pairing mechanism to activate a short upstream open reading frame to which the pqsR gene is translationally coupled. Expression of phrS requires the oxygen-responsive regulator ANR. Thus, PhrS is the first bacterial sRNA that provides a regulatory link between oxygen availability and quorum sensing, which may impact on oxygen-limited growth in P. aeruginosa biofilms. ...
Resistant Pseudomonas Aeruginosa Infections Drugs Market Insights: Global Industry Analysis, Market Drivers, Restraints, Opportunities, Applications, Trends And Forecasts 2020-2026
Summary Pharmaceutical and Healthcare disease pipeline guide Resistant Pseudomonas aeruginosa Infections - Pipeline Review, H1 2017, provides an overview o
Anti Pseudomonas aeruginosa Serotype 8 Antibody, clone 1017/87 , Mouse Anti-Bacterial Monoclonal Antibody validated in WB (ABD12646), Abgent
Pseudomonas aeruginosa is an ubiquitous organism. Its ability to survive on minimal nutritional requirements and to tolerate a variety of physical conditions allows its persistence in both community and hospital settings [12]. P. aeruginosa is a serious therapeutic challenge for treatment of both community-acquired and nosocomial infections, due to the ability of this microorganism to develop resistance to multiple classes of antibacterial agents, even during the course of therapy [13, 14]. The increasing frequency of MDR or XDR P. aeruginosa strains is of concern as effective antimicrobial options are limited [15, 16]. Moreover, only a few new antibiotics are currently under development [6]. An increase in MDR bacterial infections among companion animals has been documented in multiple veterinary hospital settings [17]. This is of particular importance due to the risk of transmission to humans and other companion animals in close contact with infected animals, even because in our countries the ...
The Gram-negative pathogen Pseudomonas aeruginosa is a significant burden on the health care industry, with up to 10% of nosocomial infections attributed to this pathogen (1, 2). These infections can present as acute and chronic infections of burn wounds, skin, and indwelling medical devices and can disseminate, resulting in sepsis (1, 2). However, chronic pulmonary infections caused by P. aeruginosa are the most prevalent threat to the health and well-being of the nations 30,000 cystic fibrosis (CF) patients, with more than 80% of adult patients harboring these infections (3). Patients are initially infected with nonmucoid environmental strains of P. aeruginosa; however, over time, mutations in mucA, encoding an anti-sigma factor result in the overproduction of the polysaccharide alginate, termed mucoidy (4). Isolation of mucoid strains from sputum is associated with the transition from intermittent to chronic infection and poor outcomes (5, 6). In addition to the underlying disease and direct ...
Persistent lung infection by Pseudomonas aeruginosa is typically associated with the development of biofilms, the appearance of morphotypic variants and reduction in the expression of acute virulence factors. We have characterised and compared functional traits [carbon substrate utilisation, attachment and biofilm formation, protease and elastase activity, quorum-sensing (QS)] of the biofilm dispersal populations of a representative P. aeruginosa isolate from a chronically infected cystic fibrosis individual and P. aeruginosa strain PAO1. The dispersal variants of the clinical strain exhibited significantly greater heterogeneity in all of the phenotypes tested. All morphotypic variants from the dispersal population of the clinical strain showed a significant increase in QS signal and elastase production compared to the parental strain. In contrast, isolates from planktonic cultures were phenotypically identical to the inoculum strain, suggesting that the appearance of these variants was biofilm ...
BACKGROUND: Pseudomonas aeruginosa sinusitis may be the focus for intermittent lung colonization in patients with cystic fibrosis (CF). The sinusitis may induce elevated IgA levels in nasal secretion and saliva against P. aeruginosa. METHODS: 120 CF patients chronically infected, intermittently colonized or without P. aeruginosa in the lungs participated in this cross-sectional study. IgA and IgG against P. aeruginosa sonicate and alginate were measured in nasal secretions, saliva, and in serum by ELISA. RESULTS: The intermittently colonized patients had significantly higher IgA levels in nasal secretions and saliva than those without P. aeruginosa in the lungs, indicating that P. aeruginosa sinusitis may precede intermittent colonization and chronic infection of the lungs. CONCLUSIONS: Specific IgA against P. aeruginosa in nasal secretions and saliva can contribute to differentiation between patients chronically infected, intermittently colonized, and without P. aeruginosa in the lungs. The ...
TY - JOUR. T1 - Application of WGS data for O-specific antigen analysis and in silico serotyping of Pseudomonas aeruginosa isolates. AU - Thrane, Sandra Wingaard. AU - Taylor, Véronique L.. AU - Lund, Ole. AU - Lam, Joseph S.. AU - Jelsbak, Lars. PY - 2016. Y1 - 2016. N2 - Accurate typing methods are required for efficient infection control. The emergence of whole genome sequencing (WGS) technologies has enabled the development of genomics-based methods applicable for routine typing and surveillance of bacterial pathogens. In this study, we developed the Pseudomonas aeruginosa serotyper (PAst) program, which enabled in silico serotyping of P. aeruginosa isolates using WGS data. PAst has been made publically available as a web-service, and aptly facilitate high-throughput serotyping analysis. The program overcomes critical issues such as the loss of in vitro typeability often associated with P. aeruginosa isolates from chronic infections, and quickly determines the serogroup of an isolate based ...
Pseudomonas aeruginosa infections is of public health concerns due to high morbidities and mortalities often occasioned by its multidrug resistant nature, particularly to drugs like fluoroquinolones and cephalosporin. Constant surveillance and tracking of its possible sources of transmission is therefore of epidemiological value. We screened 30 Pseudomonas aeruginosa isolated from oral and rectum swabs of rats captured from some poultry houses in Nigeria; identified by conventional bacteriological procedures and confirmed with 16S ribosomal RNA PCR assay, for their antibiotic susceptibilities to 10 commonly used antibiotics in Nigeria based on standard method. The fluoroquinolone resistant strain were subsequently screened for point mutation at the GyrA of the quinolone resistant determining region(QRDR) through DNA amplification and sequencing. They exhibited 100% resistance at breakpoint concentrations for; ceftazidime, ceftriaxone, sulfamethoxazole, chloramphenicol, streptomycin and ...
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Vaccins pour la prévention de linfection par la Pseudomonas aeruginosa dans les cas de mucoviscidose. La mucoviscidose est une maladie héréditaire dans laquelle un mucus épais est secrété dans les poumons. La Pseudomonas aeruginosa et dautres bactéries sont responsables dinfections pulmonaires durables qui causent des dommages irréversibles aux poumons. Des vaccins visant à réduire linfection par la Pseudomonas aeruginosa ont été développés et il est important de savoir si la vaccination est capable de prévenir linfection du poumon. Nous avons recherché des essais contrôlés randomisés et avons inclus trois essais impliquant respectivement 483, 476 et 37 patients. Aucune donnée nétait disponible pour lun des grands essais, qui navait pas été publié. Dans lautre grand essai et dans le petit essai, le risque de contracter une infection chronique navait pas diminué. Dans le grand essai, le décès dun patient avait été signalé durant la période dobservation. ...
Digit amputation is a commonly performed surgery in cattle practice. The most frequent reason for digit amputation is septic pedal arthritis, but other indications include severe trauma, pedal osteitis, non-healing sole, wall or toe ulcers, osteomyelitis of P2, septic tenosynovitis of the distal digital flexor tendons and other infectious processes of the deep digital structures. The procedure is considered a salvage procedure, but good rates of success are reported. The size of the patient is one of the key determinants of prognosis, with reported success rates varying between bulls, cows and calves. Reported complications include haemorrhage, avascular necrosis of the phalangeal fragments, wound infection and fracture of the partner claw. In this case report the authors describe digit amputation under regional and general anaesthesia for the treatment of proximal interphalangeal septic arthritis and the subsequent wound management in the face of a multidrug resistant Pseudomonas aeruginosa ...
Pseudomonas aeruginosa cells were selected for their ability to grow in the presence of 750 ppm alkyldimethylethylbenzyl ammonium chloride (QAC). These cells were found to retain their resistance to the germicide throughout tri-weekly transfers for 7 months in tryptone glucose yeast extract (TGY) broth containing no QAC. Comparisons of the resistant and sensitive cells were made in an attempt to define the mechanism of resistance and, in addition, to provide some information as to the mode of action of QAC. The germicidal activity of QAC solutions against both sensitive and resistant cells in TGY broth was shown to be greatly affected by the concentration of tryptone and yeast extract, but not by the amount of sugar. The pH of the broth also influenced the germicidal activity; both strains were more susceptible under slightly . acid conditions. A comparison of the pH range of growth of sensitive and resistant cells demonstrated the ability of the former to grow in TGY broth at pH 4.5 while the ...
Multidrug resistant (MDR) Pseudomonas aeruginosa has caused serious nosocomial infections owing to its high intrinsic resistance and ease of acquiring resistance to common antibiotics. There is an urgent need to develop antimicrobial agents against MDR Pseudomonas aeruginosa. Here we report a 27-mer peptide polymer
Silver nanoparticles (AgNPs), have been widely used as antibacterial therapy for any microorganisms that are multidrug resistance to antibiotics. Pseudomonas aeruginosa is the most common respiratory pathogen in patients with cystic fibrosis (CF), was collected from Al-Muthanna hospitals. These isolates were drug-resistant against tetracycline, chloramphenicol, erythromycin, streptomycin, azithromycin and trimethoprim, while they were sensitive to imipenem. The conventional PCR was used to screen for many different virulent genes and eventually, the only algD and plcH were detected among 11 Pseudomonas aeruginosa strains over of twenty isolates of P. aeruginosa isolated from patients with cystic fibrosis (CF) disease. The Ag/F Tio2 NPs was used as antibacterial to test the AgNPs activity against the expression of algD and plcH genes that are screened as a common complication of gene virulence in the cystic fibrosis (CF) disease. The results showed a significant effect on algD and plcH genes ...
TY - UNPB. T1 - The high persister phenotype of Pseudomonas aeruginosa is associated with increased fitness and persistence in cystic fibrosis airways. AU - Mojsoska, Biljana. AU - Cameron, David R.. AU - Bartell, Jennifer A.. AU - Haagensen, Janus A.J.. AU - Sommer, Lea M.. AU - Lewis, Kim. AU - Molin, Søren. AU - Johansen, Helle Krogh. PY - 2019/2/27. Y1 - 2019/2/27. N2 - Pseudomonas aeruginosa (P.a) is responsible for the majority of chronic lung infections in patients suffering from cystic fibrosis (CF) [1]. Despite intensive treatment with antibiotics from the first discovery of this bacterium in the patient lungs, and despite the fact that only few cases of antibiotic resistance are observed the first many years, these bacteria persist and adapt, and eventually develop incurable chronic airway infections in most patients. Persister bacteria are susceptible cells that survive antibiotic treatment and can resume growth when antibiotics are no longer present, resulting in antibiotic ...
The expression of chromosomal AmpC β-lactamase in Pseudomonas aeruginosa is negatively regulated by the activity of an amidase, AmpD. In the present study we examined resistant clinical P. aeruginosa strains and several resistant variants isolated from in vivo and in vitro biofilms for mutations in ampD to find evidence for the genetic changes leading to high-level expression of chromosomal β-lactamase. A new insertion sequence, IS1669, was found located in the ampD genes of two clinical P. aeruginosa isolates and several biofilm-isolated variants. The presence of IS1669 in ampD resulted in the expression of high levels of AmpC β-lactamase. Complementation of these isolates with ampD from the reference P. aeruginosa strain PAO1 caused a dramatic decrease in the expression of AmpC β-lactamase and a parallel decrease of the MIC of ceftazidime to a level comparable to that of PAO1. One highly resistant, constitutive β-lactamase-producing variant contained no mutations in ampD, but a point ...
Pseudomonas aeruginosa and Acinetobacter spp. are found to be associated with biofilm and metallo-β-lactamase production and are the common causes of serious infections mainly in hospitalized patients. So, the main aims of this study were to determine the rates of biofilm production and metallo beta-lactamase production (MBL) among the strains of Pseudomonas aeruginosa and Acinetobacter spp. isolated from hospitalized patients. A total of 85 P. aeruginosa isolates and 50 Acinetobacter spp. isolates isolated from different clinical specimens from patients admitted to Shree Birendra Hospital, Kathmandu, Nepal from July 2013 to May 2014 were included in this study. The bacterial isolates were identified with the help of biochemical tests. Modified Kirby-Bauer disc diffusion technique was used for antimicrobial susceptibility testing. Combined disc diffusion technique was used for the detection of MBL production, while Congo red agar method and tube adherence method were used
Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has determined the development of new strategies in order to elucidate the different mechanisms used by these bacteria at different stages of the infectious process, providing the scientists with new procedures for preventing, or at least improving, the control of S. aureus and Ps. aeruginosa infections. The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens. We used multiplex and uniplex PCR assays to detect the genes encoding different cell-wall associated and extracellular virulence factors, in order to evaluate potential associations between the presence of putative virulence genes and the outcome of
Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has determined the development of new strategies in order to elucidate the different mechanisms used by these bacteria at different stages of the infectious process, providing the scientists with new procedures for preventing, or at least improving, the control of S. aureus and Ps. aeruginosa infections. The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens. We used multiplex and uniplex PCR assays to detect the genes encoding different cell-wall associated and extracellular virulence factors, in order to evaluate potential associations between the presence of putative virulence genes and the outcome of
Pseudomonas aeruginosa is a Gram negative aerobic rod shaped bacterium and is an opportunistic pathogen that usually causes nosocomial infection in immunocompromised patient with various infections and affects normal healthy human as well. P. aeruginosa is also an omnipresent pathogen that can be inhabited in soil, water, vegetable, human and animal. Metallo-β-lactamases (MBL) producing P. aeruginosa are known to be resistant to almost the entire anti-pseudomonas agent via mechanism of low outer membrane permeability, β-lactamases synthesis and the efflux systems. This study was conducted to detect the potential of metallo-β-lactamases producing P. aeruginosa presence in water samples from various parts of Malaysia. In this study, 52 water samples were collected from various parts of Malaysia. These P. aeruginosa isolates were processed to these phenotypic methods, Hodge test which is used to detect the carbapenemase production, Imipenem- EDTA combined disc test (CDT). Imipenem-EDTA
The galactose specific lectin LecA partly mediates the formation of antibiotic resistant biofilms by Pseudomonas aeruginosa, an opportunistic pathogen causing lethal airways infections in immunocompromised and cystic fibrosis patients, suggesting that preventing LecA binding to natural saccharides might prov Multivalent Biomolecular Recognition
TY - JOUR. T1 - Activation of NF-κB by adherent Pseudomonas aeruginosa in normal and cystic fibrosis respiratory epithelial cells. AU - DiMango, E.. AU - Ratner, A. J.. AU - Bryan, R.. AU - Tabibi, S.. AU - Prince, A.. PY - 1998/6/1. Y1 - 1998/6/1. N2 - PMN-dominated airway inflammation is a major component of cystic fibrosis (CF) lung disease. Epithelial cells respond to organisms such as Pseudomonas aeruginosa, the major pathogen in CF, by expressing the leukocyte chemokine IL-8. Experiments were performed using several different types of respiratory epithelial cells that demonstrate that ligation of ceramide- associated receptors on epithelial surfaces by P. aeruginosa pili is a major stimulus for the translocation of transcription factor nuclear factor (NF)- κB and initiation of IL-8 expression by epithelial cells. Using electrophoretic mobility shift assays and Western hybridizations, nuclear NF- κB was found shortly after epithelial cells were stimulated by either whole organisms, ...
Resistance to extended-spectrum cephalosporins complicates treatment of Pseudomonas aeruginosa infections. To elucidate risk factors for cefepime-resistant P. aeruginosa and determine its association with patient death, we conducted a case-control study in Philadelphia, Pennsylvania. Among 2,529 patients hospitalized during 2001-2006, a total of 213 (8.4%) had cefepime-resistant P. aeruginosa infection. Independent risk factors were prior use of an extended-spectrum cephalosphorin (p<0.001), prior use of an extended-spectrum penicillin (p = 0.005), prior use of a quinolone (p<0.001), and transfer from an outside facility (p = 0.01). Among those hospitalized at least 30 days, mortality rates were higher for those with cefepime-resistant than with cefepime-susceptible P. aeruginosa infection (20.2% vs. 13.2%, p = 0.007). Cefepime-resistant P. aeruginosa was an independent risk factor for death only for patients for whom it could be isolated from blood (p = 0.001). Strategies to counter its
Journal of Lipids is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to all aspects of lipids, including their biochemistry, synthesis, function in health and disease, and clinical nutrition. As an interdisciplinary journal, Journal of Lipids aims to provide a forum for scientists, physicians, nutritionists, and other relevant health professionals.
Read "Conserved genomes of ΦKMV-like bacteriophages (T7 supergroup) active on Pseudomonas aeruginosa, Russian Journal of Genetics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Biofilm formation is a universal virulence strategy in which bacteria grow in dense microbial communities enmeshed within a polymeric extracellular matrix that protects them from antibiotic exposure and the immune system. Pseudomonas aeruginosa is an archetypal biofilm-forming organism that utilizes a biofilm growth strategy to cause chronic lung infections in cystic fibrosis (CF) patients. The extracellular matrix of P. aeruginosa biofilms is comprised mainly of exopolysaccharides (EPS) and DNA. Both mucoid and nonmucoid isolates of P. aeruginosa produce the Pel and Psl EPS, each of which have important roles in antibiotic resistance, biofilm formation, and immune evasion. Given the central importance of the EPS for biofilms, they are attractive targets for novel anti-infective compounds. In this study, we used a high-throughput gene expression screen to identify compounds that repress expression of the pel genes. The pel repressors demonstrated antibiofilm activity against microplate and flow ...
The ability of culture filtrate (CF) of two laboratory Pseudomonas aeruginosa strains to inhibit delayed type hypersensitivity (DTH) to non-bacterial antigen in CBA mice has been studied. It was shown that intraperitoneal injection of native CF of the strains did not affect the level of DTH. However, redox treated CF expressed the immunosuppressive activity. Gel filtration of redox activated CF through Sephadex G-200 showed that CF contains three immunosuppressive components differing by their molecular weight and specificity. All components contained lipid group and O-polysaccharide chains that indicated their lipopolysaccharide (LPS) nature. These experiments show that laboratory P. aeruginosa strains have three LPS components but not all of them display the immunosuppressive activity.. ...
Pseudomonas aeruginosa ATCC ® 15442D-5™ Designation: Genomic DNA from Pseudomonas aeruginosa strain PRD-10 TypeStrain=False Application: Food testing
The binding of CO to ascorbate-reduced Pseudomonas cytochrome oxidase was investigated by static-titration, stopped-flow and flash-photolytic techniques. Static-titration data indicated that the binding process was non-stoicheiometric, with a Hill number of 1.44. Stopped-flow kinetics obtained on the binding of CO to reduced Pseudomonas cytochrome oxidase were biphasic in form; the faster rate exhibited a linear dependence on CO concentration with a second-order rate constant of 2 × 10(4) M-1-s-1, whereas the slower reaction rapidly reached a pseudo-first-order rate limit at approx. 1s-1. The relative proportions of the two phases observed in stopped-flow experiments also showed a dependency on CO concentration, the slower phase increasing as the CO concentration decreased. The kinetics of CO recombination after flash-photolytic dissociation of the reduced Pseudomonas cytochrome oxidase-CO complex were also biphasic in character, both phases showing a linear pseudo-first-order rate dependence ...
Human tear fluid protects against Pseudomonas aeruginosa keratitis in a murine experimental model.: Pseudomonas aeruginosa keratitis is an acute sight-threateni
The present Competitive Intelligence Report about Pseudomonas aeruginosa Vaccines & Therapeutics provides a competitor evaluation in the field of prophylactic
We have discovered that P. aeruginosa produces a cell-to-cell signal that is unlike any previously reported intercellular signal molecule. This molecule was determined to have a 4-quinolone base structure and therefore has been designated as the Pseudomonas quinolone signal (PQS). Our results showed that exogenously added PQS induced a lasB′-lacZ fusion in the P. aeruginosa lasR mutant, strain PAO-R1, containing the plasmid pTS400 (Fig. 2A). This indicates that there is a third cell-to-cell signal, in addition to the autoinducers 3-oxo-C12-HSL and C4-HSL, that is involved in lasB induction by P. aeruginosa. Despite the ability of PQS to activate lasB′-lacZ in the absence of lasR, the production of PQS required an active LasR protein (Fig. 2A). This suggested that a gene (or genes) required for PQS synthesis is controlled through the las quorum sensing system. We also found that at least RhlR was required for PQS to act as a signal because lasB′-lacZ was not induced by PQS in the lasR, rhlR ...
Biofilm infection is now understood to be a potent contributor to the recalcitrant nature of chronic wounds. Bacterial biofilms evade the host immune response and show increased resistance to antibiotics. Along with improvements in antibiotic stewardship, effective new anti-biofilm therapies are urgently needed for effective wound management. Previous studies have shown that bioactive glass (Bg) is able to promote healing with moderate bactericidal activity. Here we tested the antimicrobial efficacy of a novel BG incorporating silver (BgAg), against both planktonic and biofilm forms of the wound-relevant bacteria Pseudomonas aeruginosa and Staphylococcus aureus. BgAg was stable, long lasting, and potently effective against planktonic bacteria in time-kill assays (6-log reduction in bacterial viability within 2 h) and in agar diffusion assays. BgAg reduced bacterial load in a physiologically relevant ex vivo porcine wound biofilm model; P. aeruginosa (2-log reduction) and S. aureus (3-log ...
Biofilms are multicellular communities formed by densely-packed microbes that are often associated with persistent infections. Steep gradients of nutrients and oxygen form in these crowded structures. The human pathogen Pseudomonas aeruginosa produces molecules called phenazines that help it to cope with the oxygen-limited conditions within biofilms. Columbia researchers have uncovered new roles for proteins of the electron transport chain that implicate them in utilization of phenazines. Illustration by Nicoletta Barolini.. ...
Biofilms are multicellular communities formed by densely-packed microbes that are often associated with persistent infections. Steep gradients of nutrients and oxygen form in these crowded structures. The human pathogen Pseudomonas aeruginosa produces molecules called phenazines that help it to cope with the oxygen-limited conditions within biofilms. Columbia researchers have uncovered new roles for proteins of the electron transport chain that implicate them in utilization of phenazines. Illustration by Nicoletta Barolini.. ...
Abstract : In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has beennoticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedisbiofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa. Five MDR clinical lab isolates (LS 22, LS 23, LS38, LS 47, and LS 58) of P. aeruginosa were taken and divided into two groups i.e. control and biofield treated.Control and treated group were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration(MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed 60% alteration insensitivity of tested antimicrobials in MDR isolates of P. aeruginosa after biofield treatment. MIC results showed analteration in 42.85% tested antimicrobials out of twenty eight after biofield treatment in ...
P. aeruginosa rarely causes disease in healthy humans. It is usually linked with patients whose immune system is compromised by diseases or trauma. It gains access to these patients tissues through the burns, for the burn victims, or through an underlying disease, like cystic fibrosis. First, P. aeruginosa adheres to tissue surfaces using its flagellum, pili, and exo-S; then, it replicates to create infectious critical mass; and lastly, it makes tissue damage using its virulence factors (21). Since the powerful exotoxins and endotoxins released by P. aeruginosa during bacteremias continue to infect the host even after P. aeruginosa has been killed off by antibiotics, acute diseases caused by P. aeruginosa tend to be chronic and life-threatening. Furthermore, with the exception of the cystic fibrosis strain, most P. aeruginosa strains that attack compromised patients tend to be nonmucoid (2). And even though a small amount of patients infected by P. aeruginosa developed severe sepsis with ...
P. aeruginosa rarely causes disease in healthy humans. It is usually linked with patients whose immune system is compromised by diseases or trauma. It gains access to these patients tissues through the burns, for the burn victims, or through an underlying disease, like cystic fibrosis. First, P. aeruginosa adheres to tissue surfaces using its flagellum, pili, and exo-S; then, it replicates to create infectious critical mass; and lastly, it makes tissue damage using its virulence factors (21). Since the powerful exotoxins and endotoxins released by P. aeruginosa during bacteremias continue to infect the host even after P. aeruginosa has been killed off by antibiotics, acute diseases caused by P. aeruginosa tend to be chronic and life-threatening. Furthermore, with the exception of the cystic fibrosis strain, most P. aeruginosa strains that attack compromised patients tend to be nonmucoid (2). And even though a small amount of patients infected by P. aeruginosa developed severe sepsis with ...
Debate regarding the co-existence of Staphylococcus aureus and Pseudomonas aeruginosa in wounds remains contentious, with the dominant hypothesis describing a situation akin to niche partitioning, whereby both microorganisms are present but occupy distinct regions of the wound without interacting. In contrast, we hypothesised that these microorganisms do interact during early co-colonisation in a manner beneficial to both bacteria. We assessed competitive interaction between S. aureus and P. aeruginosa in biofilm cultured for 24-72 h and bacterial aggregates analogous to those observed in early (,24h) biofilm formation, and interaction with human keratinocytes. We observed that S. aureus predominated in biofilm and non-attached bacterial aggregates, acting as a pioneer for the attachment of P. aeruginosa. We report for the first time that S. aureus mediates a significant (P,0.05) increase in the attachment of P. aeruginosa to human keratinocytes, and that P. aeruginosa promotes an invasive ...
Purpose of reviewRecent articles of clinical interest on Pseudomonas aeruginosa respiratory tract infections including CAP, nosocomially-acquired pneumonia, particularly in the ventilated patient, and chronic infections in cystic fibrosis patients are reviewed.Recent findingsThe growing importance o
Twenty-three patients (85.2%) were infected with MDR P. aeruginosa, confirmed with cultures and resistance tests, during the ICU stay. In greater part, they were isolated from respiratory and urinary tract infections (33.3% and 25.9%, respectively). Four patients were treated empirically, with 50% of therapy response. The study group presented a mean age of 63 years, 51.9% males, with a mean Acute Physiology and Chronic Health Evaluation II score of 24.63. Sixty-three percent of our patients were first admitted to the hospital with community-acquired infection, none caused by P. aeruginosa. The most frequent cause for ICU admission was communitarian or nosocomial respiratory tract infection (29.9%). The mean time of polimixin B use was 15.59 days. Twelve patients (44%) used imipinem, 12 patients (44%) used teicoplanin and three patients (11%) used vancomicin, for more than 3 days, during the ICU stay before polimixin B use. After treatment with polimixin B, we had 40.7% response and improvement ...
The LysR-family regulator MexT modulates the expression of the MexEF-OprN efflux system in the human pathogen Pseudomonas aeruginosa. Recently, we demonstrated that MexT regulates certain virulence phenotypes, including the type-three secretion system and early attachment independent of its role in regulating MexEF-OprN. In this study, transcriptome profiling was utilized to investigate the global nature of MexT regulation in P. aeruginosa PAO1 and an isogenic mexEF mutant. Twelve genes of unknown function were highly induced by overexpressing MexT independent of MexEF-OprN. A well-conserved DNA motif was identified in the upstream regulatory region of nine of these genes and upstream of mexE. Reporter fusion analysis demonstrated that the expression of the genes was significantly induced by MexT in P. aeruginosa and a heterogenous Escherichia coli strain and that the conserved sequence was required for this induction. The conserved DNA motif was further characterized as the MexT binding site by ...
Pseudomonas aeruginosa ExaE protein: a response regulator of a two-component regulatory system for controling expression quinoprotein ethanol dehydrogenase; isolated from Pseudomonas aeruginosa; amino acid sequence in first source
Anti-Pseudomonas aeruginosa Serotype 2B Antibody, clone 1253/17 , Mouse Anti-Bacterial Monoclonal Antibody validated in E (ABD10730), Abgent
Abstract : Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedis biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics. Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using automated MicroScan Walk-Away® system on day 10. The treated sample was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship, the epidemiological relatedness and genetic characteristics. Results: Data showed altered sensitivity pattern in antibiotic cefotaxime from intermediate to decreased β-lactamases ...
Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen, which causes serious debilitating infections in patients with compromised lung function. The mechanism by which P. aeruginosa is cleared from the lung is not fully defined, although our previous studies have established a role for cellular immunity in protection against P. aeruginosa infections. This study aimed to evaluate the role of P. aeruginosa-specific IgG in protection against P. aeruginosa in a rat model of acute pulmonary infection. Immunoaffinity chromatography was used to purify total rat IgG from rat immune serum (rats immunised with P. aeruginosa) and non-immune serum. Untreated recipient rats were injected intravenously with different concentrations of pure IgG prepared from serum of unimmunised rats (non-immune IgG) or from rats immunised intestinally with killed P. aeruginosa (immune IgG) and infected intratracheally with P. aeruginosa 18 h later. The protective capability of the purified IgG against P. aeruginosa ...
RESULTS: As a result of the antibiotic resistance analysis, 11 different antibiotypes for Hospital 1 and Hospital 3, while 6 different antibiotypes for Hospital 2 were determined. The highest incidence of MDR strains was observed in Hospital 1. It was found that MDR strains were frequently isolated from patients who underwent surgical interventions or patients in the intensive care units. As a result of genotyping, 9 different genotypes were determined in Hospital 1, 7 in Hospital 2, and 17 in Hospital 3. There was no significant correlation between the antibiotypes and genotypes. A clonal relationship between the MDR strains that were isolated from both the same service and from different services was observed in Hospital 1. These findings support the hypothesis that the strains are spread within the hospital via cross or horizontal transmission through hospital employees or medical instruments. According to Simpsons diversity index, the RAPD-PCR methods discrimination power was found as 0.92 ...
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Mouse monoclonal Pseudomonas aeruginosa serotype 9 antibody [95/159] validated for ELISA, ICC/IF. Immunogen corresponding to tissue, cells or virus
Wright, Laura, Turton, Jane F., Hopkins, Katie L, Livermore, David and Woodford, Neil (2015) Genetic environment of metallo-β-lactamase genes in Pseudomonas aeruginosa isolates from the UK. Journal of Antimicrobial Chemotherapy, 70 (12). pp. 3250-3258. ISSN 0305-7453 ...
The Gram-negative opportunistic pathogen Pseudomonas aeruginosa ubiquitously inhabits soil and water habitats and also causes serious, often antibiotic resistant, infections in immunocompromised patients (e.g. cystic fibrosis). This versatility is mediated in part by a large repertoire of two-compon …
Pseudomonas aeruginosa PvdE protein: required for pyoverdine biosynthesis in Pseudomonas aeruginosa; amino acid sequence in first source; GenBank U07359
Read "Comparisons of the Genomes of New Giant Phages Isolated from Environmental Pseudomonas aeruginosa Strains of Different Regions, Russian Journal of Genetics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Purpose : Pseudomonas aeruginosa is a leading cause of gram-negative keratitis, particularly in contact lens wearers and readily forms biofilms on biotic an abiotic surfaces, including contact lenses. Biofilms are thought to play a role in potentiating corneal infection, which has implications for treatment strategies in an era of increasing antibiotic resistance. We sought to demonstrate increased resistance of Pseudomonas clinical isolates to common ophthalmic antibiotics when grown in biofilms on synthetic surfaces, including contact lenses. Methods : Seven keratitis P. aeruginosa strains were collected from the Flaum Eye Institute and compared to the laboratory strain PAO1. Phenotypic characteristics such as color, colony morphology and biofilm formation were noted. Virulence factors, exoS, exoY, exoU, exoT and pcrV were determined by PCR. Minimum inhibitory concentrations (MIC) to a panel of ophthalmic antibiotics were measured by determining colony-forming-units (CFUs) after a 24-hour ...
Some clinical isolates of Pseudomonas aeruginosa stored in our culture collection did not grow or grew poorly and showed lysis on the culture plates when removed from the collection and inoculated on MacConkey agar. One hypothesis was that bacteriophages had infected and killed those clinical isolates. To check the best storage conditions to maintain viable P. aeruginosa for a longer time, clinical isolates were stored at various temperatures and were grown monthly. We investigated the presence of phage in 10 clinical isolates of P. aeruginosa stored in our culture collection. Four strains of P. aeruginosa were infected by phages that were characterized by electron microscopy and isolated to assess their ability to infect. The best condition to maintain the viability of the strains during storage was in water at room temperature. Three Siphoviridae and two Myoviridae phages were visualized and characterized by morphology. We confirmed the presence of bacteriophages infecting clinical isolates, ...
TY - JOUR. T1 - OXA-46, a new class D β-lactamase of narrow substrate specificity encoded by a blaVIM-1-containing integron from a Pseudomonas aeruginosa clinical isolate. AU - Giuliani, Francesco. AU - Docquier, Jean Denis. AU - Riccio, Maria Letizia. AU - Pagani, Laura. AU - Rossolini, Gian Maria. PY - 2005/5. Y1 - 2005/5. N2 - A novel OXA-type enzyme, named OXA-46, was found to be encoded by a gene cassette inserted into a class 1 integron from a multidrug-resistant Pseudomonas aeruginosa clinical isolate. The variable region of the integron also contained a blaVIM-1 metallo-β-lactamase cassette and a duplicated aacA4 aminoglycoside acetyltransferase cassette. OXA-46 belongs to the OXA-2 lineage of class D β-lactamases. It exhibits 78% sequence identity with OXA-2 and the highest similarity (around 92% identity) with another OXA-type enzyme detected in clinical isolates of Burkholderia cepacia and in unidentified bacteria from a wastewater plant. Expression of blaOXA-46 in Escherichia coli ...
PURIFIKASI DAN KARAKTERISTIK PROTEASE DARI PATOGEN Pseudomonas aeruginosa[Purification and Charaterization of Protease from Pathogenic Bacteria Pseudomonas aeruginosa]Ace Baekhari 1), Maggy T. Suhartono 2), Nurheni Sri Palupi 2) dan Tati Nurhayati 3)1) Program Studi Teknologi Hasil Perikanan, Fakultas Pertanian Universitas Sriwijaya2) Departemen Ilmu dan Teknologi Pangan, Institut Pertanian Bogor3) Departemen Teknologi Hasil Perairan, Institut Pertanian BogorDiterima…
The type IV pili (T4P) is a major virulence factor of Pseudomonas aeruginosa (P. aeruginosa) that is associated with primary adhesion, biofilm formation and twitching motility. This study focuses on the introduction of a novel biologically active subunit vaccine derived from the disulfide loop (DSL) of P. aeruginosa pilin. We investigated the ...
Pseudomonas aeruginosa bacteria inside biofilm, computer illustration. This is a Gram-negative, aerobic, enteric, rod prokaryote. P. aeruginosa causes skin infections, urinary tract infections and septicaemia. It produces a blue-green pigment, pyocyanin, which characterizes the bluish pus produced by the infection. It is found in soil, water, skin flora, and most man-made environments throughout the world. Many strains are antibiotic-resistant and produce biofilms. A biofilm is a colony of bacteria that forms a coating on a surface. Common places for biofilms of P. aeruginosa to develop are on contact lenses, where they can cause eye infections, on catheters, where they cause catheter-associated infections, and on wound surfaces, where they cause wound infections. - Stock Image F018/1267
The aim of this study was to assess the influence of some abiotic factors monitoring abundances of Pseudomonas aeruginosa, in open and closed wells in sandy and clayey-lateritic soils in Cameroon (Central Africa). In closed wells, the abundance of P. aeruginosa varied from 1 to 153 CFU/100 ml in sandy soil, and from 1 to 60 CFU/100 ml in clayey-lateritic soil. In open wells, it varied from 1 to 200 CFU/100 ml in sandy soil, and from 1 to 58 CFU/100 ml in clayey-lateritic soil. Abundances of P. aeruginosa underwent temporal variations in wells. Meteorological, physicochemical and hydrological factors impacts at different magnitudes the abundance dynamic of cells. Positive correlation (P,0.05) between rainfall and P. aeruginosa abundances were more observed in wells in clayey-lateritic soil than those of sandy soil. Lower cells abundances observed in some open wells were related to the high insolation periods (P,0.05). The hierarchical organization was made by expressing percentage and ranking in ...
Other names: ATCC 10145, ATCC 10145-U, Bacillus aeruginosus, Bacillus pyocyaneus, Bacterium aeruginosum, Bacterium pyocyaneum, CCEB 481, CCUG 28447, CCUG 29297, CCUG 551, CFBP 2466, CIP 100720, DSM 50071, IBCS 277, IFO 12689, JCM 5962, Micrococcus pyocyaneus, NBRC 12689, NCCB 76039, NCIB 8295, NCIMB 8295, NCTC 10332, NRRL B-771, P. aeruginosa, Pseudomonas aeruginosa, Pseudomonas polycolor, Pseudomonas pyocyanea, Pseudomonas sp. RV3, RH 815, VKM B-588, bacterium ASFP-37, bacterium ASFP-38, bacterium ASFP-45, bacterium ASFP-46, bacterium ASFP-48 ...
Other names: ATCC 10145, ATCC 10145-U, Bacillus aeruginosus, Bacillus pyocyaneus, Bacterium aeruginosum, Bacterium pyocyaneum, CCEB 481, CCUG 28447, CCUG 29297, CCUG 551, CFBP 2466, CIP 100720, DSM 50071, IBCS 277, IFO 12689, JCM 5962, Micrococcus pyocyaneus, NBRC 12689, NCCB 76039, NCIB 8295, NCIMB 8295, NCTC 10332, NRRL B-771, P. aeruginosa, Pseudomonas aeruginosa, Pseudomonas polycolor, Pseudomonas pyocyanea, Pseudomonas sp. RV3, RH 815, VKM B-588, bacterium ASFP-37, bacterium ASFP-38, bacterium ASFP-45, bacterium ASFP-46, bacterium ASFP-48 ...
December 2, 2008 - The bacterium Pseudomonas aeruginosa is well known for its environmental versatility, ability to cause infection in humans, and its capacity to resist antibiotics. P. aeruginosa is the most common cause of persistent and fatal lung infections in cystic fibrosis patients. In a study published online today in Genome Research (www.genome.org), researchers have used genomic techniques to study a particularly virulent strain of P. aeruginosa, uncovering genetic clues to its success that will aid in the design of novel therapeutic strategies.. The Liverpool Epidemic Strain, the most common strain of P. aeruginosa infecting cystic fibrosis patients in the United Kingdom, is characterized by its particular aggressiveness and virulence. Though approximately 90% of the P. aeruginosa genome is shared between different strains, a team of scientist led by Dr. Craig Winstanley of the University of Liverpool set out to investigate the unique genomic features of the Liverpool strain. "We used ...
A method of inducing an immune response against multiple strains of Pseudomonas aeruginosa which comprises administering to a human or animal an amount of mucoid exopolysaccharide from Pseudomonas aer