Pseudomonas cichorii is a Gram-negative soil bacterium that is pathogenic to plants. It has a wide host range, and can have an important economical impact on lettuce, celery and chrysanthemum crops. P. cichorii was first isolated on endives (Cichorium endivia), from which it derives its name. It produces 6-aminopenicillanic acid. Based on 16S rRNA analysis, P. cichorii has been placed in the P. syringae group. Pseudomonas cichorii is non-host specific as it does not infect just one host. Its host range includes lettuce, pepper, celery, coffee, wheat, basil and several other host plants. Symptoms of the causal agent vary depending on the host and the area of the plant infected. In general, pseudomonas cichorii is seen to cause leaf blighting and spotting. The first appearance of symptoms involves a water soaked lesion that develops at the edge of the leaf, midvein or randomly across the leaf These lesions progressively turn black or brown and may be surrounded by yellow halos. These lesions also ...

Thirty-three fluorescent Pseudomonas strains isolated from tomato pith necrosis (FPTPN strains) and 89 Pseudomonas corrugata strains were studied by numerical taxonomy. In the dendrogram of distances, the P. corrugata strains constituted a single phenon (phenon 1), whereas 17 of the 33 FPTPN strains clustered in a separate phenon (phenon 2). The other 16 FPTPN strains were included in phena consisting of well-characterized fluorescent Pseudomonas species or were isolated phenotypes. Phena 1 and 2 were distinguished by fluorescence on King B medium, accumulation of poly-β;-hydroxybutyrate, production of levan, and assimilation of sorbitol. DNA-DNA hybridization showed that P. corrugata is a true genomic species (66 to 100% DNA relatedness) and that the FPTPN strains of phenon 2 were divided into three genomic groups. Genomic groups 1 and 2 were not distinct from each other phenotypcally, and genomic group 3 could be distinguished from genomic groups 1 and 2 only on the basis of assimilation of

TY - JOUR. T1 - The LuxR regulators PcoR and RfiA co-regulate antimicrobial peptide and alginate production in Pseudomonas corrugata. AU - Bella, Patrizia. AU - Licciardello, Grazia. AU - Trantas, Emmanouil A.. AU - Sarris, Panagiotis F.. AU - Strano, Cinzia P.. AU - Catara, Vittoria. AU - Caruso, Andrea. AU - Caruso, Andrea. AU - Anzalone, Alice. AU - Gheleri, Rodolpho. AU - Caruso, Andrea. AU - Almeida, Nalvo F.. PY - 2018. Y1 - 2018. N2 - Cyclic lipopeptides (CLPs) are considered as some of the most important secondary metabolites in different plant-associated bacteria, thanks to their antimicrobial, cytotoxic, and surfactant properties. In this study, our aim was to investigate the role of the Quorum Sensing (QS) system, PcoI/PcoR, and the LuxR-type transcriptional regulator RfiA in CLP production in the phytopatogenic bacterium, Pseudomonas corrugata based on our previous work where we reported that the pcoR and rfiA mutants were devoid of the CLPs cormycin and corpeptin production. Due to ...

Pseudomonas viridiflava is a pectinolytic bacterium member of the Pseudomonas syringae group (1). It is pathogenic to numerous cultivated crops and weeds (2), including Arabidopsis thaliana, in which it induces both compatible (disease) and incompatible (resistance) responses (3). For this reason, it has triggered much interest in plant-microbe interaction studies in A. thaliana (4, 5).. Pathogenicity genes and mechanisms are becoming increasingly well-known, and 2 paralogous pathogenicity islands (T-PAI and S-PAI), which share many gene homologs, have been described for P. viridiflava (6, 7).. P. viridiflava was shown to display a high level of genetic variation worldwide, with all isolated P. viridiflava strains parting into two distinct and deeply diverged clades, with evidence of frequent recombination but little geographic differentiation (4, 5). These 2 distinct clades cause disease symptoms of differing severities.. This bacterium is an antimycotic producer that is usable in biological ...

The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT exhibited much higher UV sensitivity than did P. putida strains. UV-induced mutagenesis of plasmid-free P. putida was easily detected in three different assays (two reversion assays and one forward mutation assay), whereas UV mutagenesis of P. aeruginosa without CAM-OCT was seen only in the forward mutation assay. These results suggest major differences in DNA repair between the two species and highlight the presence of error-prone repair functions on CAM-OCT. A number of P. putida mutants carrying chromosomal mutations affecting either survival or mutagenesis after UV irradiation were isolated, and the effect of CAM-OCT on these mutants was determined. All mutations producing a ...

The chromosomal DNA was isolated and purified from 17 strains of Pseudomonas paucimobilis, and from the type or reference strains of Flavobacterium capsulatum, F. devorans, F. multivorum, Chromobacterium lividum, Xanthomonas campestris and seven species of Pseudomonas. The DNA base compositions (mol%G + C) of P. paucimobilis strains were between 62·2 and 68·6%, and typical strains had a mean value of 65·3 ± 0·4 mol %, determined from thermal denaturation temperature. DNA-DNA molecular hybridization with 3H-labelled probe DNA from NCTC 11030 P. paucimobilis (the type strain) indicated that the species comprised a core of 13 closely related strains (74 to 96%), which included F. devorans NCIB 8195 (= ATCC 10829). Four P. paucimobilis strains displayed lower levels of hybridization (≤ 38%). The hybridization results showed that P. paucimobilis was not closely related to allied yellow-pigmented bacteria or to other reference pseudomonads. The electrophoretic protein patterns of representative

Pseudomonas amygdali is a Gram-negative plant pathogenic bacterium. It is named after its ability to cause disease on almond (Prunus amygdalus) trees. Different analyses, including 16S rRNA analysis, DNA-DNA hybridization, and MLST clearly placed P. amygdali in the P. syringae group together with the species Pseudomonas ficuserectae and Pseudomonas meliae, and 27 pathovars of Pseudomonas syringae/Pseudomonas savastanoi, constituting a single, well-defined phylogenetic group which should be considered as a single species. This phylogenetic group has not been formally named because of the lack of reliable means to differentiate it phenotipically from closely related species, and it is currently known as either genomospecies 2 or phylogroup 3. When it is formally named, the correct name for this new species should be Pseudomonas amygdali, which takes precedence over all the other names of taxa from this group, including Pseudomonas savastanoi, which is and inadequate and confusing name whose use is ...

Université de Liège - ULg , Département des sciences biomédicales et précliniques , Microbiologie médicale et virologie médicale ,] ...

If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. Thank you!. Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016 ...

Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Cobalt atom in PDB 1h41: Pseudomonas Cellulosa E292A Alpha-D-Glucuronidase Mutant Complexed With Aldotriuronic Acid

The broad and vague phenotypic definition allowed the genus Pseudomonas to become a dumping ground for incompletely characterized polarly flagellated, gram-negative, rod-shaped, aerobic bacteria, and a large number of species have been accommodated in the genus Pseudomonas. The 16S rRNA sequences of 128 valid and invalid Pseudomonas species, which included almost valid species of the genus Pseudomonas listed in the Approved Lists of Bacterial Names, were obtained: sequences of 59 species were determined and those of 69 species were obtained from the GenBank/EMBL/DDBJ databases. These sequences were compared with the sequences of other species of the Proteobacteria. Fifty-seven valid or invalid species including Pseudomonas aeruginosa (type species of the genus Pseudomonas Migula 1894) belonged to the genus Pseudomonas (sensu stricto). Seven subclusters were formed in the cluster of the genus Pseudomonas (sensu stricto), and the resulting clusters conformed well to the rRNA-DNA hybridization study by

One way of identifying and categorizing multiple bacterial organisms in a sample is to use ribotyping.[37] In ribotyping, differing lengths of chromosomal DNA are isolated from samples containing bacterial species, and digested into fragments.[37] Similar types of fragments from differing organisms are visualized and their lengths compared to each other by Southern blotting or by the much faster method of polymerase chain reaction (PCR).[37] Fragments can then be matched with sequences found on bacterial species.[37] Ribotyping is shown to be a method to isolate bacteria capable of spoilage.[38] Around 51% of Pseudomonas bacteria found in dairy processing plants are P. fluorescens, with 69% of these isolates possessing proteases, lipases, and lecithinases which contribute to degradation of milk components and subsequent spoilage.[38] Other Pseudomonas species can possess any one of the proteases, lipases, or lecithinases, or none at all.[38] Similar enzymatic activity is performed by Pseudomonas ...

One way of identifying and categorizing multiple bacterial organisms in a sample is to use ribotyping.[42] In ribotyping, differing lengths of chromosomal DNA are isolated from samples containing bacterial species, and digested into fragments.[42] Similar types of fragments from differing organisms are visualized and their lengths compared to each other by Southern blotting or by the much faster method of polymerase chain reaction (PCR).[42] Fragments can then be matched with sequences found on bacterial species.[42] Ribotyping is shown to be a method to isolate bacteria capable of spoilage.[43] Around 51% of Pseudomonas bacteria found in dairy processing plants are P. fluorescens, with 69% of these isolates possessing proteases, lipases, and lecithinases which contribute to degradation of milk components and subsequent spoilage.[43] Other Pseudomonas species can possess any one of the proteases, lipases, or lecithinases, or none at all.[43] Similar enzymatic activity is performed by Pseudomonas ...

TY - JOUR. T1 - Infections caused by Pseudomonas species in patients with burns and in other surgical patients. AU - Pruitt, Basil A.. PY - 1974/11. Y1 - 1974/11. N2 - Infections caused by Pseudomonas species are a particular threat to all patients with extensive burns, as well as to other critically ill surgical patients. These gramnegative opportunistic bacteria proliferate rapidly in and invade through nonviable tissue such as the burn wound. Topical chemotherapy with Sulfamylonburn creams has significantly reduced the occurrence of pseudomonas burn wound sepsis, but wound surveillance and biopsy monitoring are essential to the assessment of the bacterial density of the burn and to the timely alteration of therapy. Pseudomonas infections of the lung may be either hematogenous or airborne in origin, and the clinical course and treatment vary accordingly. Pseudomonas suppurative thrombophlebitis may also occur in patients with impaired antimicrobial defenses and may serve as a source of ...

Define pseudomonas. pseudomonas synonyms, pseudomonas pronunciation, pseudomonas translation, English dictionary definition of pseudomonas. n. pl. pseu·do·mon·a·des Any of various gram-negative, rod-shaped, mostly aerobic flagellated bacteria of the genus Pseudomonas, commonly found in soil,...

TY - JOUR. T1 - Lipodepsipeptides from Pseudomonas syringae are partially proteolyzed and are not absorbed by humans. T2 - an in vitro study. AU - Fiore, A.. AU - Laparra, J. M.. AU - Farre, R.. AU - Fullone, M. R.. AU - Grgurina, I.. AU - Gallo, M.. AU - Fogliano, V.. PY - 2008/5. Y1 - 2008/5. N2 - There are some concerns about the use of Pseudomonas-based products as biocontrol agents because of the hemolytic activity shown by their metabolites. The effects of Pseudomonas lipodepsipeptides (LDPs) on mammals via ingestion and the LDP degradation during the digestion and intestinal permeability have not been evaluated. In this research, the susceptibility of different LDPs to degradation was assayed with enzymatic gastrointestinal digestion, and intestinal permeability to LDPs was investigated in an in vitro system based on an intestinal cell layer system. Results demonstrated that trypsin and chymotrypsin hydrolyze up to 50% of the various LDPs, and that proteolysis was further increased by ...

Other names: ATCC 8062, CCUG 2087, CFBP 5589, CIP 59.11, IFO 13583, JCM 11598, LMG 2229, NBRC 13583, NCIMB 6576, NCTC 10692, NRRL B-778, P. oleovorans, Pseudomonas oleovorans, Pseudomonas sp. MGY01 ...

Other names: ATCC 8062, CCUG 2087, CFBP 5589, CIP 59.11, IFO 13583, JCM 11598, LMG 2229, NBRC 13583, NCIMB 6576, NCTC 10692, NRRL B-778, P. oleovorans, Pseudomonas oleovorans, Pseudomonas sp. MGY01 ...

Pseudomonas aeruginosa bacteria, computer illustration. P. aeruginosa is a gram-negative bacterium which causes multiple antibiotic resistant nosocomial (hospital-acquired) infections of different location, including pneumonia, osteomyelitis, peritonitis and wound infections. - Stock Image F012/9762

General Information: This strain was isolated from agricultural loam (sand, clay, and organic matter) soil in 1988 by Compeau et al. and is well adapted to soil environments. Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. This organism is a nonpathogenic saprophyte which inhabits soil, water and plant surface environments. If iron is in low supply, it produces a soluble, greenish fluorescent pigment, which is how it was named. As these environmentally versatile bacteria possess the ability to degrade (at least partially) multiple different pollutants, they are studied in their use as bioremediants. ...

General Information: This strain was isolated from agricultural loam (sand, clay, and organic matter) soil in 1988 by Compeau et al. and is well adapted to soil environments. Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. This organism is a nonpathogenic saprophyte which inhabits soil, water and plant surface environments. If iron is in low supply, it produces a soluble, greenish fluorescent pigment, which is how it was named. As these environmentally versatile bacteria possess the ability to degrade (at least partially) multiple different pollutants, they are studied in their use as bioremediants. ...

In this study, the efficient phosphate utilizing isolates were used to remove phosphate from synthetic phosphate wastewater was tested using batch scale process. Hence the objective of the present study was to examine the efficiency of bacterial species individually for the removal of phosphate from synthetic phosphate wastewater. The most efficient phosphate reducers were isolated and screened from eutrophic lake water samples. The total heterotrophic bacterial analysis of the samples showed the presence of about 22 phosphate reducers. Among them, Pseudomonas sp YLW-7 were found to be efficient in phosphate reduction based on the maximum phosphate ultization which was observed by plate screening method using the minimum inhibitory concentration (MIC) test. The effect of carbon sources (glucose, starch, sucrose and lactose) at 0.5% on the removal of phosphate by Pseudomonas sp YLW7 was estimated. The maximum growth of Pseudomonas sp YLW7 was observed to be 0.9886 OD in glucose followed by starch ...

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Penyalver, R., Bertolini, E., Olmos, A., Garcia, A., Cambra, M., Lopez, M.M. (2001). Detection of Pseudomonas savastanoi pv. savastanoi (Pss), on asymptomatic olive plant tissues by enrichment-PCR. , , 424 ...

Pseudomonas species are opportunistic pathogens with implications in a wide range of diseases including cystic fibrosis and sickle cell anaemia. Because of their status as multidrug resistant (MDR) and extremely drug resistant (XDR) bacteria Pseudomonas species represent a threat to public health. Prevalence, antibiogram and associated antibiotic resistant genes of Pseudomonas species isolated from freshwater and mixed liquor environments in the Eastern Cape Province of South Africa were assessed. Polymerase chain reaction (PCR) based technique was used to identify the isolates and screen for antibiotic resistant genes. The result shows occurrence of Pseudomonas spp. in freshwater and mixed liquor as follows: 71.42% and 37.5% (P. putida), 14.28% and 31.25% (P. flourescens), 7.14% and 6.25% (P. aeruginosa) and 7.14% and 25% for other Pseudomonas species respectively. Disk diffusion antibiogram of the Pseudomonas isolates from the two locations showed 100% resistance to penicillin, oxacillin, clindamycin,

This study analysed the phenotypic and genotypic variation among 511 Pseudomonas savastanoi pv. phaseolicola (Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean (Vigna radiata) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phenotypically screened. Our in planta screening against a set of four mungbean cultivars with known susceptible and resistant reactions revealed five distinctive pathotypes. Isolates belonging to pathotype 2 were the most prevalent at 84% and were found to be highly pathogenic towards all tested mungbean genotypes. Genomic variation was investigated for 205 isolates using DNA fingerprints, splitting the halo blight pathogen population into two broad genetic lineages. Further genetic testing for two known avirulence genes, avrPphE and avrPphF, identified the avrPphE gene in all the tested isolates and avrPphF present in all but two. To identify candidate avirulence genes unique to Psp isolates infecting ...

Plant Disease 97:1381.1-1381.1...Plant Disease 97:1381.1-1381.1...First Report of Tomato Pith Necrosis (Pseudomonas corrugata) on Tomato (Solanum lycopersicum) in Washington...M. Powell , B. Gundersen , and C. A. Miles , Departments of Plant Pathology and Horticulture, Washington State University Mount Vernon NWREC, 16650 State Route 536, Mount Vernon 98273 ; J. L. Humann and B. K. Schroeder , Department of Plant Pathology, Washi...

Looking for online definition of Pseudomonas facilis in the Medical Dictionary? Pseudomonas facilis explanation free. What is Pseudomonas facilis? Meaning of Pseudomonas facilis medical term. What does Pseudomonas facilis mean?

TY - JOUR. T1 - Isolation and structural elucidation of syringostatins, phytotoxins produced by pseudomonas syringae pv. syringae lilac isolate. AU - Fukuchi, Naoyuki. AU - Isogai, Akira. AU - Nakayama, Jiro. AU - Takayama, Seiji. AU - Yamashita, Shuichi. AU - Suyama, Kazuo. AU - Suzuki, Akinori. PY - 1992. Y1 - 1992. N2 - A bacterial strain of Pseudomonas syringae pv. syringae isolated from lilac was found to produce a homologous mixture of phytotoxins different from syringomycin and syringotoxin. The toxins were termed syringostatins and the structures of the main components, syringostatins A and B, were determined by 2D-NMR spectroscopy and mass spectrometry. Minor component structures were elucidated from mass/mass spectra.. AB - A bacterial strain of Pseudomonas syringae pv. syringae isolated from lilac was found to produce a homologous mixture of phytotoxins different from syringomycin and syringotoxin. The toxins were termed syringostatins and the structures of the main components, ...

TY - JOUR. T1 - Analysis of the role of the Pseudomonas syringae pv. syringae HrpZ harpin in elicitation of the hypersensitive response in tobacco using functionally non-polar hrpZ deletion mutations, truncated HrpZ fragments, and hrmA mutations. AU - Alfano, James R.. AU - Bauer, David W.. AU - Milos, Timothy M.. AU - Collmer, Alan. PY - 1996. Y1 - 1996. N2 - Pseudomonas syringae pv. syringae, like many plant pathogenic bacteria, secretes a harpin protein that can elicit the hypersensitive response (HR), a defensive cellular suicide, in non-host plants. The harpin-encoding hrpZ gene is located in an operon that also encodes Hrp secretion pathway components and is part of the functional cluster of hrp genes carried on cosmid pHIR11 that enables saprophytic bacteria like Escherichia coli and Pseudomonas fluorescens to elicit the HR in tobacco leaves. We have constructed functionally non-polar hrpZ deletion mutations, revealing that HrpZ is necessary for saprophytic bacteria carrying pHIR11 to ...

Pseudomonas syringae pathovar phaseolicola ATCC ® BAA-978D™ Designation: Genomic DNA from Pseudomonas syringae pathovar phaseolicola strain 1448A TypeStrain=False Application:

Pseudomonas syringae is pathogenic in a wide variety of plants, causing diseases with economic impacts. Pseudomonas syringae pathovars produce several toxins that can function as virulence factors and contribute to disease symptoms. These virulence factors include antimetabolite toxins, such as tabtoxin, phaseolotoxin and mangotoxin, which target enzymes in the pathways of amino acid metabolism. The antimetabolite toxins are generally located in gene clusters present in the flexible genomes of specific strains. These gene clusters are typically present in blocks of genes that appear to be integrated into specific sites in the P. syringae core genome. A general overview of the genetic organization and biosynthetic and regulatory functions of these genetic traits of the antimetabolite toxins will be given in the present work.

TY - JOUR. T1 - The hrpK operon of Pseudomonas syringae pv. tomato DC3000 encodes two proteins secreted by the type III (Hrp) protein secretion system. T2 - HopB1 and HrpK, a putative type III translocator. AU - Petnicki-Ocwieja, Tanja. AU - Van Dijk, Karin. AU - Alfano, James R.. PY - 2005/1. Y1 - 2005/1. N2 - Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant cells for pathogenicity. The P. syringae TTSS is encoded by hrp-hrc genes that reside in a central region of a pathogenicity island (Pai). Flanking one side of this Pai is the exchangeable effector locus (EEL). We characterized the transcriptional expression of the open reading frames (ORFs) within the EEL of P. syringae pv. tomato DC3000. One of these ORFs, PSPTO1406 (hopB1) is expressed in the same transcriptional unit as hrpK. Both HopB1 and HrpK were secreted in culture and translocated into plant cells ...

Pseudomonas syringae pv. savastanoi y P. syringae pv. phaseolicola son dos patógenos de plantas, incluidos en los 60 patovares del grupo P. syringae. Las dos bacterias causan enfermedad en diferentes huéspedes y con síntomas muy distintos, la primera tumores en olivo y la segunda lesiones en judía, y son organismos modelos de estudio en la identificación de los determinantes que definen el espectro de huésped. La definición del espectro de huésped puede estar determinada por la acción conjunta de proteínas llamadas efectores, que son secretadas por un sistema de secreción tipo III a la célula vegetal, dando lugar a la inhibición de las respuestas de defensa de la planta. El objetivo del trabajo ha sido la identificación de genes de efectores de P. syringae pv. savastanoi que induzcan respuesta de incompatibilidad en judía, para lo que se abordó la clonación y ensayo de 11 efectores. De éstos, sólo se pudieron obtener clones de los efectores AER-0000629 y AER-0001936, que se ...

Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics and proteomics studies with other P. putida strains, the P. putida KT2440 genomic database serves as standard reference. The utility of KT2440 whole-genome, high-density oligonucleotide microarrays for transcriptomics studies of other Pseudomonas strains was investigated. To this end, microarray hybridizations were performed with genomic DNAs of subcultures of P. putida KT2440 (DSM6125), the type strain (DSM291T), plasmid pWW0-containing KT2440-derivative strain mt-2 (DSM3931), the solvent-tolerant P. putida S12, and several other Pseudomonas strains. Depending on the strain tested, 22 to 99% of all genetic elements were identified in the genomic DNAs. The efficacy of these microarrays to study cellular function was determined for all strains included in the study. The vast majority of DSM6125 genes encoding proteins of primary metabolism and genes involved in the catabolism of aromatic compounds ...

Carbapenems are critically important antimicrobials as a last line of defense against multidrug-resistant Gram-negative bacterial infections (1, 2). As such, the increasing prevalence of carbapenemase-producing isolates in animal husbandry is of great concern. While the metallo-β-lactamase (MBL)-producing bacteria have been commonly identified from food animals (3-7), blaMBL-carrying Pseudomonas spp. are rarely reported in animal husbandry or the surrounding environment. Although we have reported the high prevalence of NDM in Enterobacteriaceae isolates from poultry production in Shandong Province (7), carbapenemase-producing non-Enterobacteriaceae isolates have not been identified in the same region. Here, we report four chromosome-borne VIM-positive Pseudomonas isolates: one Pseudomonas aeruginosa isolate from a swallow (Yanornis martini), one Pseudomonas putida isolate from a fly, and two P. putida isolates from chickens. The blaVIM-2 gene was identified in the P. aeruginosa isolate, but 27 ...

Synonyms for blepharitis marginalis in Free Thesaurus. Antonyms for blepharitis marginalis. 3 words related to blepharitis: inflammation, redness, rubor. What are synonyms for blepharitis marginalis?

TY - JOUR. T1 - Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate. T2 - Insight into the basis of its substrate specificity. AU - Kim, Youngsoo. AU - Hol, Wim G.J.. N1 - Funding Information: We thank Ethan Merritt, Stephen Suresh and Craig Behnke for their helpful discussions, Jungwoo Choe, Stewart Turley and Mic Feese for collecting data, and the SBC-CAT staff, in particular Randy Alkire, Stephan Ginell and Rongguang Zhang for their technical assistance on the APS SBC-CAT beamline. Use of the Argonne National Laboratory Structural Biology Center beamlines at the APS was supported by the US Department of Energy Office of Energy Research, under contract No. W-31-109-ENG-38. We also thank Francis Athappilly, Irwin Hirsh, and Claudia Roach of the Biomolecular Structure Center for maintaining our computer facilities and helping with the protein expression, purification and crystallization. W.G.J.H. acknowledges a major equipment grant from the ...

Fruits is a scientific journal for original articles and reviews on fruit crops in temperate, Mediterranean, subtropical and tropical regions

Tytuł projektu: Udostępnianie cyfrowe zasobów polskich czasopism z nauk przyrodniczych i rolniczych w bazie AGRO. Nr umowy: POPC.02.03.01-00-0038/18-00 (okres realizacji 2018-2021). Kwota dofinansowania: 7 442 980,00 z. W ramach Programu Operacyjnego Polska Cyfrowa na lata 2014-2020, Oś Priorytetowa nr 2 E-administracja i otwarty rząd Działanie nr 2.3 Cyfrowa dostępność i użyteczność informacji sektora publicznego Poddziałanie nr 2.3.1 Cyfrowe udostępnienie informacji sektora publicznego ze źródeł administracyjnych i zasobów nauki (typ projektu: cyfrowe udostępnienie zasobów nauki) Instytucja Finansująca: Centrum Projektów Polska Cyfrowa ...

Competition among indigenous and inoculated 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was studied in a native Kansas prairie soil following 2,4-D additions. The soil was inoculated with four different 2,4-D-degrading strains at densities of 10(3) cells per g of soil; the organisms used were Pseudomonas cepacia DBO1(pJP4) and three Michigan soil isolates, strain 745, Sphingomonas paucimobilis 1443, and Pseudomonas pickettii 712. Following 2,4-D additions, total soil DNA was extracted and analyzed on Southern blots by using a tfdA gene probe which detected three of the strains and another probe that detected the fourth strain, S. paucimobilis 1443, which belongs to a different class of 2,4-D degraders. P. cepacia DBO1(pJP4), a constructed strain, outcompeted the other added strains and the indigenous 2,4-D-degrading populations. The S. paucimobilis population was the secondary dominant population, and strain 745 and P. pickettii were not detected. Relative fitness coefficients determined

Bacterial stem blight of alfalfa occurs sporadically in the central and western U.S. Yield losses of up to 50% of the first harvest can occur with some cultivars. Developing resistant cultivars is hampered by lack of information on the pathogen and a standard test for evaluating plant germplasm. Bacteria producing a fluorescent pigment were isolated on Kings B agar from alfalfa with symptoms of bacterial stem blight from near Cheyenne, WY. The strain ALF3 was tentatively identified as Pseudomonas syringae pv. syringae based on 16S rDNA sequence and PCR amplification of syrB for lipodepsinonapeptide toxin production. Multilocus sequence analysis indicated that ALF3 falls within a clade containing strains of P. syringae pv. syringae with closest affinity to FF5 from pear. Comparison of a draft whole-genome sequence of ALF3 further confirmed that ALF3 most closely resembles FF5 (~96% sequence identity) and P. syringae pv. aptata DSM50252 from beet. Approximately 60 genes were unique to ALF3, ...

TY - JOUR. T1 - Immunological detection of syringopeptins produced by Pseudomonas syringae pv. lachrymans. AU - Fogliano, V.. AU - Gallo, M.. AU - Vinale, F.. AU - Ritieni, A.. AU - Randazzo, G.. AU - Greco, M.. AU - Lops, R.. AU - Graniti, A.. PY - 1999/11. Y1 - 1999/11. N2 - Several strains of plant pathogenic Pseudomonas are known to produce phytotoxic lipodepsipeptides (syringomycin, syringopeptins and related compounds) in vitro. However, detection of these compounds in organic extracts from diseased plant tissues has been attempted by chromatographic methods for syringomycin only. A macromolecular derivative of syringopeptins (KLH-SP(25A+B)) was used to raise polyclonal antibodies in rabbit. The antiserum was able to recognize free syringopeptins (SP22 and SP25) with an estimated detection limit of 0.05 μg per well in the indirect ELISA, and 0.01 μg per well in the competitive ELISA. Cross-reaction with other structurally related lipodepsipeptides, e.g. syringomycins and pseudomycin A, ...

TY - THES. T1 - Genetic aspects of resistance to Pseudomonas solanacearum E.F. Smith in potato. AU - Tung, P.X.. N1 - WU thesis 1506 Proefschrift Wageningen. PY - 1992. Y1 - 1992. N2 - ,TT,The genetic control of resistance toPseudomonas s,TT,olanacearum in potato is complex and may involve both genes with major effects and genes with minor effects. However, no evidence of a gene-for-gene relationship between the host and the pathogen has been as yet documented. Strain specificity in the potato-P. solanacearum pathosystem is of the polygenic quantitative type and is probably a reflection of differential adaptation of host genotype and pathogen genotype to environments. Thus the resistance is chacterized by strong host x pathogen x environment interaction and tends to break down whenever faced with environmental conditions the host is not well adapted to. Expression of the resistance is heavily dependent on the adaptive potential of the carrier host genotype to a particular environment. Under heat ...

Bacteria that inhabit the rhizosphere of agricultural crops can have a beneficial effect on crop growth. One such mechanism is the microbial-driven solubilisation and remineralisation of complex forms of phosphorus (P). It is known that bacteria secrete various phosphatases in response to low P conditions. However, our understanding of their global proteomic response to P stress is limited. Here, exoproteomic analysis of Pseudomonas putida BIRD-1 (BIRD-1), Pseudomonas fluorescens SBW25 and Pseudomonas stutzeri DSM4166 was performed in unison with whole-cell proteomic analysis of BIRD-1 grown under phosphate (Pi) replete and Pi deplete conditions. Comparative exoproteomics revealed marked heterogeneity in the exoproteomes of each Pseudomonas strain in response to Pi depletion. In addition to well-characterised members of the PHO regulon such as alkaline phosphatases, several proteins, previously not associated with the response to Pi depletion, were also identified. These included putative ...

ADP-ribosylation of proteins occurs in many eukaryotes, and it is also the mechanism of action of a growing number of important bacterial toxins. To date, however, there is only one well-characterized ADP-ribosylation system where the ADP-ribosyltransferase and the substrate protein are both bacterial in origin, namely within the nitrogen-fixing bacterium Rhodospirillum rubrum. The present paper demonstrates the endogenous ADP-ribosylation of two proteins of Mr 32,000 and 20,000 within Pseudomonas maltophilia, a Gram-negative aerobe. The proteins have been partially purified: two apparently separate species of modified protein can be separated by ion-exchange chromatography and gel filtration (V0 and Mr 158,000 - Vi). The substrate protein(s) either has, or is co-eluted with, NAD+ glycohydrolase activity. The modification is mono-ADP-ribosyl in nature. The linkage between the acceptor amino acid and the ADP-ribose moiety is alkali-labile and stable to hydroxylamine, possibly indicating an ...

Pseudomonas chlororaphis ATCC ® 55670™ Designation: TX-1 TypeStrain=False Application: Biological control of turfgrass pathogens Biological control of Sclerotinia homoeocarpa

Wang, Dongping; Dorosky, Robert; Han, Cliff; Lo, Chien-chi; Dichosa, Armand; Chain, Patrick; Jun Myoung Yu; Pierson, Leland; III; Pierson, Elizabeth (2015). Adaptation Genomics of a Small-Colony Variant in a Pseudomonas chlororaphis 30-84 Biofilm. Applied and Environmental Microbiology. Available electronically from http : / /hdl .handle .net /1969 .1 /182425. ...

Biodegradation in water: Experimental study and predicted data for the target compound (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one (CAS No. 89-80-5) and varioussupporting studiesfor its structurally similar read across substance were reviewed for the biodegradation end point which are summarized as below: In an experimental key study from peer reviewed journal (1995), biodegradation experiment was conducted for 21 days for evaluating the percentage biodegradability of test substance (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one (CAS no. 89-80-5) by using Pseudomonas citronellolis DSM 50332 as an inoculum. Test inoculum Pseudomonas citronellolis DSM 50332 was obtained from the Deutsche Sammlung von Mikroorganismen, Braunschweig, Germany. Initial test substance conc. used for the study was 308.5 mg/l (2 mM). Anoxic media was used for the study.The medium contained (per liter of distilled water) 1 g of NaCl, 0.1 g of MgCl2.7H2O, 0.04 g of CaCl2, 0.5 g of KCl, 0.125 g of NH4Cl, 0.2 g of ...

This site uses Akismet to reduce spam. 2. In many cases, Pseudomonas infections are preventable. Septicaemia People with existing lung diseases sometimes carry the bacteria in their lungs without causing infection. A doctor may also prescribe an antibiotic called polymyxin. Certain symptoms depend on the site at which the disease occurs. Produce acid from xylose. Pseudomonas bacteria are generally harmless. These include ear infections and skin rashes, especially after exposure to water. Although it rarely causes disease in healthy individuals, it is a significant threat to hospitalized patients, especially those with severe underlying conditions such as cancer and burns. Of the many different types of Pseudomonas, the one that most often causes infections in humans is called Pseudomonas aeruginosa, which can cause infections in the blood, lungs (pneumonia), or other parts of the body after surgery. It is a widespread free-living bacterium and is found in most moist environments such as skin ...

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Pseudomonas syringae pv. DC3000 is a gram-negative bacterium that infects the model plant Arabidopsis thaliana. Pathogenicity is achieved via secretion of effector proteins into the host cytoplasm through a Type III Secretion System (T3SS). In Ps. DC3000 the T3SS (and associated effector proteins) are dependent on HrpL for their transcription. hrpL transcription is sigma54-dependent and requires two co-dependent enhancer binding proteins, HrpR and HrpS (HrpRS), for activation. HrpRS are regulated by two hrpL-dependent proteins, HrpV and HrpG, where HrpV negatively affects HrpRS activity and HrpG relieves this repression. Here the mechanism of HrpV and HrpGs action on HrpRS activity was tested in vivo and in vitro; and the molecular determinants of HrpV and HrpG functionality were characterised by in silico and mutational analysis. Whole-gene deletion mutants of hrpV and hrpG in Ps. DC3000 revealed complications associated with inserting marker cassettes in transcriptionally-antagonistic ...

Autor: Gupta, K. J. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2013; Open Access; Titel: The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco

Author: Gupta, K. J. et al.; Genre: Journal Article; Published in Print: 2013; Open Access; Title: The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco

This invention relates to the production and use of recombinant Pseudomonas-derived toxins modified to increase their toxicity and potency in therapy. More particularly, the invention relates to certain deletions in domain II of the amino acid sequence of Pseudomonas exotoxin the domain which relates to the toxins natural proteolytic processing.

The enzyme is involved in production of the rare amino acid 3-methylarginine, which is used by the epiphytic bacterium Pseudomonas syringae pv. syringae as an antibiotic against the related pathogenic species Pseudomonas syringae pv. glycinea ...

Coloured scanning electron micrograph (SEM) of Pseudomonas putida, Gram-negative, aerobic, enteric, rod prokaryote. Pseudomonas putida is a ubiquitous soil bacterium. Strains of P. putida have the ability to degrade organic solvents or hydrocarbons. This strain was isolated from a soil environment that had high levels of caffeine. It is known to biodegrade caffeine. Thus different strains of Pseudomonas putida can be used for bioremediation. Magnification: x1,600 when shortest axis printed at 25 millimetres. - Stock Image C032/1985

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