Pseudomonas cichorii is a Gram-negative soil bacterium that is pathogenic to plants. It has a wide host range, and can have an important economical impact on lettuce, celery and chrysanthemum crops. P. cichorii was first isolated on endives (Cichorium endivia), from which it derives its name. It produces 6-aminopenicillanic acid. Based on 16S rRNA analysis, P. cichorii has been placed in the P. syringae group. Pseudomonas cichorii is non-host specific as it does not infect just one host. Its host range includes lettuce, pepper, celery, coffee, wheat, basil and several other host plants. Symptoms of the causal agent vary depending on the host and the area of the plant infected. In general, pseudomonas cichorii is seen to cause leaf blighting and spotting. The first appearance of symptoms involves a water soaked lesion that develops at the edge of the leaf, midvein or randomly across the leaf These lesions progressively turn black or brown and may be surrounded by yellow halos. These lesions also ...
Pseudomonas viridiflava is a pectinolytic bacterium member of the Pseudomonas syringae group (1). It is pathogenic to numerous cultivated crops and weeds (2), including Arabidopsis thaliana, in which it induces both compatible (disease) and incompatible (resistance) responses (3). For this reason, it has triggered much interest in plant-microbe interaction studies in A. thaliana (4, 5).. Pathogenicity genes and mechanisms are becoming increasingly well-known, and 2 paralogous pathogenicity islands (T-PAI and S-PAI), which share many gene homologs, have been described for P. viridiflava (6, 7).. P. viridiflava was shown to display a high level of genetic variation worldwide, with all isolated P. viridiflava strains parting into two distinct and deeply diverged clades, with evidence of frequent recombination but little geographic differentiation (4, 5). These 2 distinct clades cause disease symptoms of differing severities.. This bacterium is an antimycotic producer that is usable in biological ...
Pseudomonas amygdali is a Gram-negative plant pathogenic bacterium. It is named after its ability to cause disease on almond (Prunus amygdalus) trees. Different analyses, including 16S rRNA analysis, DNA-DNA hybridization, and MLST clearly placed P. amygdali in the P. syringae group together with the species Pseudomonas ficuserectae and Pseudomonas meliae, and 27 pathovars of Pseudomonas syringae/Pseudomonas savastanoi, constituting a single, well-defined phylogenetic group which should be considered as a single species. This phylogenetic group has not been formally named because of the lack of reliable means to differentiate it phenotipically from closely related species, and it is currently known as either genomospecies 2 or phylogroup 3. When it is formally named, the correct name for this new species should be Pseudomonas amygdali, which takes precedence over all the other names of taxa from this group, including Pseudomonas savastanoi, which is and inadequate and confusing name whose use is ...
Université de Liège - ULg , Département des sciences biomédicales et précliniques , Microbiologie médicale et virologie médicale ,] ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Cobalt atom in PDB 1h41: Pseudomonas Cellulosa E292A Alpha-D-Glucuronidase Mutant Complexed With Aldotriuronic Acid
The broad and vague phenotypic definition allowed the genus Pseudomonas to become a dumping ground for incompletely characterized polarly flagellated, gram-negative, rod-shaped, aerobic bacteria, and a large number of species have been accommodated in the genus Pseudomonas. The 16S rRNA sequences of 128 valid and invalid Pseudomonas species, which included almost valid species of the genus Pseudomonas listed in the Approved Lists of Bacterial Names, were obtained: sequences of 59 species were determined and those of 69 species were obtained from the GenBank/EMBL/DDBJ databases. These sequences were compared with the sequences of other species of the Proteobacteria. Fifty-seven valid or invalid species including Pseudomonas aeruginosa (type species of the genus Pseudomonas Migula 1894) belonged to the genus Pseudomonas (sensu stricto). Seven subclusters were formed in the cluster of the genus Pseudomonas (sensu stricto), and the resulting clusters conformed well to the rRNA-DNA hybridization study by
One way of identifying and categorizing multiple bacterial organisms in a sample is to use ribotyping.[37] In ribotyping, differing lengths of chromosomal DNA are isolated from samples containing bacterial species, and digested into fragments.[37] Similar types of fragments from differing organisms are visualized and their lengths compared to each other by Southern blotting or by the much faster method of polymerase chain reaction (PCR).[37] Fragments can then be matched with sequences found on bacterial species.[37] Ribotyping is shown to be a method to isolate bacteria capable of spoilage.[38] Around 51% of Pseudomonas bacteria found in dairy processing plants are P. fluorescens, with 69% of these isolates possessing proteases, lipases, and lecithinases which contribute to degradation of milk components and subsequent spoilage.[38] Other Pseudomonas species can possess any one of the proteases, lipases, or lecithinases, or none at all.[38] Similar enzymatic activity is performed by Pseudomonas ...
Define pseudomonas. pseudomonas synonyms, pseudomonas pronunciation, pseudomonas translation, English dictionary definition of pseudomonas. n. pl. pseu·do·mon·a·des Any of various gram-negative, rod-shaped, mostly aerobic flagellated bacteria of the genus Pseudomonas, commonly found in soil,...
Other names: ATCC 8062, CCUG 2087, CFBP 5589, CIP 59.11, IFO 13583, JCM 11598, LMG 2229, NBRC 13583, NCIMB 6576, NCTC 10692, NRRL B-778, P. oleovorans, Pseudomonas oleovorans, Pseudomonas sp. MGY01 ...
Other names: ATCC 8062, CCUG 2087, CFBP 5589, CIP 59.11, IFO 13583, JCM 11598, LMG 2229, NBRC 13583, NCIMB 6576, NCTC 10692, NRRL B-778, P. oleovorans, Pseudomonas oleovorans, Pseudomonas sp. MGY01 ...
Pseudomonas aeruginosa bacteria, computer illustration. P. aeruginosa is a gram-negative bacterium which causes multiple antibiotic resistant nosocomial (hospital-acquired) infections of different location, including pneumonia, osteomyelitis, peritonitis and wound infections. - Stock Image F012/9762
General Information: This strain was isolated from agricultural loam (sand, clay, and organic matter) soil in 1988 by Compeau et al. and is well adapted to soil environments. Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. This organism is a nonpathogenic saprophyte which inhabits soil, water and plant surface environments. If iron is in low supply, it produces a soluble, greenish fluorescent pigment, which is how it was named. As these environmentally versatile bacteria possess the ability to degrade (at least partially) multiple different pollutants, they are studied in their use as bioremediants. ...
In this study, the efficient phosphate utilizing isolates were used to remove phosphate from synthetic phosphate wastewater was tested using batch scale process. Hence the objective of the present study was to examine the efficiency of bacterial species individually for the removal of phosphate from synthetic phosphate wastewater. The most efficient phosphate reducers were isolated and screened from eutrophic lake water samples. The total heterotrophic bacterial analysis of the samples showed the presence of about 22 phosphate reducers. Among them, Pseudomonas sp YLW-7 were found to be efficient in phosphate reduction based on the maximum phosphate ultization which was observed by plate screening method using the minimum inhibitory concentration (MIC) test. The effect of carbon sources (glucose, starch, sucrose and lactose) at 0.5% on the removal of phosphate by Pseudomonas sp YLW7 was estimated. The maximum growth of Pseudomonas sp YLW7 was observed to be 0.9886 OD in glucose followed by starch ...
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Pseudomonas oleovorans ATCC ® 8062™ Designation: TypeStrain=True Application: Assay of antimicrobial preservatives Bioresistance testing
M: 100 bp marker. Lane 1-10 shows different Pseudomonas isolates positive for the 990 bp PCR product of the universal 16S rRNA primers ...
Pseudomonas bacteria are often found in moisture environment, especially in warm places such as stagnant water. Exposure to this environmental bacteria cannot be avoided completely. In addition, there is no hard scientific evidence that avoiding activities such as swimming or showering would reduce infection with Pseudomonas. In many European countries, strict hygiene standards are imposed in public swimming pools, where the water is examined for the presence of bacteria such as Pseudomonas. With appropriate disinfection and hygiene the presence of high concentrations of Pseudomonas is unlikely. For tropical pools, it is more difficult to guarantee the quality of the water, since in very warm water and humidity, the bacteria grow faster. For that reason some centers advise not to visit such parks or subtropical swimming pools, even if there is no evidence that this actually involves risks ...
Pseudomonas syringae pv. tomato DC3000 (PtoDC3000) is an extracellular model plant pathogen, yet its potential to produce secreted effectors that manipulate the apoplast has been under investigated. Here we identified 131 candidate small, secreted, non-annotated proteins from the PtoDC3000 genome, most of which are common to Pseudomonas species and potentially expressed during apoplastic colonization. We produced 43 of these proteins through a custom-made gateway-compatible expression system for extracellular bacterial proteins, and screened them for their ability to inhibit the secreted immune protease C14 of tomato using competitive activity-based protein profiling. This screen revealed C14-inhibiting protein-1 (Cip1), which contains motifs of the chagasin-like protease inhibitors. Cip1 mutants are less virulent on tomato, demonstrating the importance of this effector in apoplastic immunity. Cip1 also inhibits immune protease Pip1, which is known to suppress PtoDC3000 infection, but has a lower
Nearly all confirmed bacterial plant pathogens are gram-negative organisms, all of which produce some form of cell wall lipopolysaccharide (LPS). Moreover, most successful pathogens or symbionts also...
Citation: N/A Interpretive Summary: Proteins with slippery properties (lipopeptides) are produced by many bacteria, which enables them to grow on surfaces that do not absorb water, such as plant leaf or stem surfaces. Bacteria have also evolved ways to make iron-binding compounds (siderophores), which allow them to grow under conditions where very little iron is available. The second example of a rarely found compound, a lipopeptide siderophore, has been discovered by German scientists in collaboration with the US Department of Agriculture (USDA). The group isolated the compound from a bacterium (Pseudomonas corrugata) that causes tomato stems to rot. They next determined the chemical structure of the compound and named it corrugatin. Bacteria in the Pseudomonas class (genus) are important because they cause many plant diseases that are responsible for millions of dollars in crop losses. The results of their cooperative work will be valuable for resolving the taxonomical placement of the tomato ...
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Pseudomonas cichorii strain LMG2163 putative HrcR (hrcR) gene, partial cds; putative HrcS (hrcS) gene, complete cds; and putative HrcT (hrcT) gene, partial ...
Pseudomonas cichorii strain LMG2164 putative HrcR (hrcR) gene, partial cds; putative HrcS (hrcS) gene, complete cds; and putative HrcT (hrcT) gene, partial ...
How do you say Pseudomonas in English? Pronunciation of Pseudomonas found 8 audio voices, 1 Meaning, 4 Sentences and 5 Synonyms for Pseudomonas.
A novel neutrophilic metalloprotease was isolated from Pseudomonas sp. DR89 isolate which was identified ina mineral spring in Iran. The enzyme was purified from the isolate to 21-folds in a three-step procedure involving ammonium sulfate precipitation, Q-Sepharose ionic exchange and Sephadex G-100 gel filtrationchromatography. Resuts showed that the enzyme was active at high temperatures and in a wide-range pH of5-11 with the optimum of 8.0. The zymogram and sodium dodecyl sulfate polyacrylamide gel electrophoresisanalysis revealed the presence of one protease with a molecular weight of 74 kDa. The enzyme activity wasdecreased by Zn2+, Mn2+, H2O2 and cetyl trimethylammonium bromide (CTAB), whereas its activity wasincreased by Ca2+, Mg2+, Cu2+ and dimethyl sulfoxide (DMSO). Na+, phenylmethyl sulfonylfluoride (PMSF),β-mercaptoethanol, sodium dodecyl sulfate (SDS), and Triton X-100 did not show a considerable effect onits activity. Casein was a better substrate than bovin serum albumin (BSA) and gelatin
Sezona sa puno kiše na više lokacija može doprineti j pojavi bakterioze na luku (Pseudomonas viridiflava). Problem je što proizvo?a?i ?esto ne razlikuju bakteriozu od mikoza. Simptomi se lako zamene sa simptomima plamenja?e. Pega izgleda vodenasto i tako dugo ostaje, a ako na njoj ima sporulacije, može biti od sekundarnih truleži, ali se onda razlikuje od sporulacije koja se javlja ... Read More ». ...
Those transfers were expected to be completed by Tuesday night.. Hospital officials emphasized that the babies who tested positive for the pseudomonas bacteria have not shown any symptoms of illness from that infection, which can be mild in healthy individuals but far more serious for those with compromised or immature immune systems.. Dimensions board members were notified by phone Sunday about the presence of what hospital officials are describing as a troubling "cluster" of the bacteria among the NICU patients, according to two individuals with direct knowledge of the phone calls.. The discovery of the bacteria followed two recent deaths in the neonatal unit, said the two people, who spoke on the condition of anonymity because they were not authorized to comment to reporters. They also said the bacteria were found in water pipes near the NICU.. Hospital officials said that the cause of the two infant deaths was still being investigated and that so far there was no evidence that the deaths ...
Phrma: 12/2/13. Throughout history, infectious diseases have taken a devastating toll on the lives and well-being of people around the world. Caused when pathogens such as bacteria or viruses enter a body and multiply, infectious diseases were the leading cause of death in the United States until the 1920s. Today, vaccines and infectious disease treatments have proven to be effective treatments in many cases, but infectious diseases still pose a very serious threat to patients. Recently, some infectious pathogens, such as pseudomonas bacteria, have become resistant to available treatments. Diseases once considered conquered, such as tuberculosis, have reemerged as a growing health threat. Read More. ...
Pseudomonas protegens ATCC ® BAA-477D-5™ Designation: Genomic DNA from Pseudomonas protegens strain Pf-5 TypeStrain=False Application:
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另外,在43株具分解辛基苯酚聚氧乙基醇能力的菌株中,Pseudomonas nitroreducens TX1生長速率最快且對辛基苯酚聚氧乙基醇的耗氧活性最高,並能生長在0.05%到20%的辛基苯酚聚氧乙基醇濃度中,其生長速率在0.34到0.44 hr-1之間。以高效能液相層析質譜儀 (HPLC-MS) 分析菌株TX1分解辛基苯酚聚氧乙基醇的代謝產物,推斷此菌株能減短聚氧乙烯鏈並生成辛基苯酚。本研究發現菌株TX1可以進一步以支鏈型辛基苯酚 (4-t-octylphenol) 為唯一碳源生長。P. nitroreducens TX1為第一株被發現同時具有分解辛基苯酚聚氧乙基醇與支鏈型辛基苯酚能力的菌株。以高效能液相層析質譜儀 (LC-ESI-Q-TOF) 分析此菌株以支鏈型辛基苯酚為唯一碳源生長過程中的代謝產物,發現有中間代謝物辛基鄰苯二酚 (4-t-octylcatechol) 生成。其中偵測到兩個經間位裂解 (meta-cleavage) 途徑產生之代謝物: ...
If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. Thank you!. Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016 ...
The genus Pseudomonasis one of the most diverse bacterial genera, containing over 60 validly described species, isolated from sources ranging from plants to contaminated soils and water to human...
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The objective of this study was to evaluate the effects of bioaugmenting anaerobic biosolids digestion with a commercial product containing selected strains of bacteria from genera Bacillus, Pseudomonas, and Actinomycetes, along with ancillary organic compounds containing various micronutrients. Spe.... Full description. ...
Pseudomonas is a Gram-negative, rod-shaped bacterium commonly found in soil, water, plants, animals and humans that has evolved to become a potentially virulent nosocomial pathogen.
The secretome of rice-infecting Pseudomonas has high proportion of dispensable genes.A) Distribution of the 729 orthologous gene clusters in the secretome accor
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|501681198|ref|YP_008001024.1| from Pseudomonas protegens CHA0. Plus protein sequence and external database links.
If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. Thank you!. Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016 ...
If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. Thank you!. Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016 ...
Study Microbiology-Legionella & Pseudomonas flashcards from John Doe's class online, or in Brainscape's iPhone or Android app. ✓ Learn faster with spaced repetition.
There are several different things that can be involved in pseudomonas testing, depending on the type of testing that is being...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Daily News How Gaining and Losing Weight Affects the Body Millions of measurements from 23 people who consumed extra calories every day for a month reveal changes in proteins, metabolites, and gut microbiota that accompany shifts in body mass.. ...
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
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Pseudomonas species are opportunistic pathogens with implications in a wide range of diseases including cystic fibrosis and sickle cell anaemia. Because of their status as multidrug resistant (MDR) and extremely drug resistant (XDR) bacteria Pseudomonas species represent a threat to public health. Prevalence, antibiogram and associated antibiotic resistant genes of Pseudomonas species isolated from freshwater and mixed liquor environments in the Eastern Cape Province of South Africa were assessed. Polymerase chain reaction (PCR) based technique was used to identify the isolates and screen for antibiotic resistant genes. The result shows occurrence of Pseudomonas spp. in freshwater and mixed liquor as follows: 71.42% and 37.5% (P. putida), 14.28% and 31.25% (P. flourescens), 7.14% and 6.25% (P. aeruginosa) and 7.14% and 25% for other Pseudomonas species respectively. Disk diffusion antibiogram of the Pseudomonas isolates from the two locations showed 100% resistance to penicillin, oxacillin, clindamycin,
Plant Disease 97:1381.1-1381.1...Plant Disease 97:1381.1-1381.1...First Report of Tomato Pith Necrosis (Pseudomonas corrugata) on Tomato (Solanum lycopersicum) in Washington...M. Powell , B. Gundersen , and C. A. Miles , Departments of Plant Pathology and Horticulture, Washington State University Mount Vernon NWREC, 16650 State Route 536, Mount Vernon 98273 ; J. L. Humann and B. K. Schroeder , Department of Plant Pathology, Washi...
Looking for online definition of Pseudomonas facilis in the Medical Dictionary? Pseudomonas facilis explanation free. What is Pseudomonas facilis? Meaning of Pseudomonas facilis medical term. What does Pseudomonas facilis mean?
Pseudomonas syringae is pathogenic in a wide variety of plants, causing diseases with economic impacts. Pseudomonas syringae pathovars produce several toxins that can function as virulence factors and contribute to disease symptoms. These virulence factors include antimetabolite toxins, such as tabtoxin, phaseolotoxin and mangotoxin, which target enzymes in the pathways of amino acid metabolism. The antimetabolite toxins are generally located in gene clusters present in the flexible genomes of specific strains. These gene clusters are typically present in blocks of genes that appear to be integrated into specific sites in the P. syringae core genome. A general overview of the genetic organization and biosynthetic and regulatory functions of these genetic traits of the antimetabolite toxins will be given in the present work.
Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics and proteomics studies with other P. putida strains, the P. putida KT2440 genomic database serves as standard reference. The utility of KT2440 whole-genome, high-density oligonucleotide microarrays for transcriptomics studies of other Pseudomonas strains was investigated. To this end, microarray hybridizations were performed with genomic DNAs of subcultures of P. putida KT2440 (DSM6125), the type strain (DSM291T), plasmid pWW0-containing KT2440-derivative strain mt-2 (DSM3931), the solvent-tolerant P. putida S12, and several other Pseudomonas strains. Depending on the strain tested, 22 to 99% of all genetic elements were identified in the genomic DNAs. The efficacy of these microarrays to study cellular function was determined for all strains included in the study. The vast majority of DSM6125 genes encoding proteins of primary metabolism and genes involved in the catabolism of aromatic compounds ...
Synonyms for blepharitis marginalis in Free Thesaurus. Antonyms for blepharitis marginalis. 3 words related to blepharitis: inflammation, redness, rubor. What are synonyms for blepharitis marginalis?
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We performed a numerical analysis of the results of biochemical and nutritional tests done with strains of nonfluorescent plant-pathogenic Pseudomonas spp. A total of 57 tests were used, and determinative tests which discriminated between taxa were identified. Pseudomonas andropogonis, P. caryophylli, P. corrugata, P. glumae, P. plantarii, and P. rubrisubalbicans were distinguished as distinct members of the genus Pseudomonas. Strains of P. ficuserectae and P. meliae formed a single cluster having affinities with P. syringae. Strains of P. cissicola were allocated to Agrobacterium spp. Strains of P. avenae, P. cattleyae, P. pseudoalcaligenes subsp. citrulli, P. pseudoalcaligenes subsp. konjaci, and P. rubrilineans formed a single relatively homogeneous cluster, within which three subclusters were discerned. Strains presently identified as P. avenae and as P. rubrilineans, including the type strain of P. cattleyae, formed a single homogeneous subcluster. Strains of P. pseudoalcaligenes subsp. citrulli
ADP-ribosylation of proteins occurs in many eukaryotes, and it is also the mechanism of action of a growing number of important bacterial toxins. To date, however, there is only one well-characterized ADP-ribosylation system where the ADP-ribosyltransferase and the substrate protein are both bacterial in origin, namely within the nitrogen-fixing bacterium Rhodospirillum rubrum. The present paper demonstrates the endogenous ADP-ribosylation of two proteins of Mr 32,000 and 20,000 within Pseudomonas maltophilia, a Gram-negative aerobe. The proteins have been partially purified: two apparently separate species of modified protein can be separated by ion-exchange chromatography and gel filtration (V0 and Mr 158,000 - Vi). The substrate protein(s) either has, or is co-eluted with, NAD+ glycohydrolase activity. The modification is mono-ADP-ribosyl in nature. The linkage between the acceptor amino acid and the ADP-ribose moiety is alkali-labile and stable to hydroxylamine, possibly indicating an ...
A novel heterotrophic, Gram-stain-negative, aerobic, rod-shaped bacterium, designated as strain MT5T, was isolated from deep seawater in the Mariana Trench and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 28 °C (range, 4-45 °C), pH 5-7 (pH 4-11) and with 3-7 % (w/v) NaCl (0-18 %). Phylogenetic analysis based on 16S rRNA gene sequence showed that strain MT5T was related to members of the genus Pseudomonas and shared the highest sequence identities with Pseudomonas pachastrellae CCUG 46540T (99.6 %), Pseudomonas aestusnigri VGXO14T (98.5 %) and Pseudomonas oceani KX 20T (98.4 %). The 16S rRNA gene sequence identities between strain MT5T and other members of the genus Pseudomonas were below 96.7 %. The digital DNA-DNA hybridization values between strain MT5T and the two type strains, P. pachastrellae and P. aestusnigri , were 38.9±2.5 and 25.8±2.4 %, respectively. The average nucleotide identity values between strain MT5T and the two type strains were
Pseudomonas syringae pv. DC3000 is a gram-negative bacterium that infects the model plant Arabidopsis thaliana. Pathogenicity is achieved via secretion of effector proteins into the host cytoplasm through a Type III Secretion System (T3SS). In Ps. DC3000 the T3SS (and associated effector proteins) are dependent on HrpL for their transcription. hrpL transcription is sigma54-dependent and requires two co-dependent enhancer binding proteins, HrpR and HrpS (HrpRS), for activation. HrpRS are regulated by two hrpL-dependent proteins, HrpV and HrpG, where HrpV negatively affects HrpRS activity and HrpG relieves this repression. Here the mechanism of HrpV and HrpGs action on HrpRS activity was tested in vivo and in vitro; and the molecular determinants of HrpV and HrpG functionality were characterised by in silico and mutational analysis. Whole-gene deletion mutants of hrpV and hrpG in Ps. DC3000 revealed complications associated with inserting marker cassettes in transcriptionally-antagonistic ...
Autor: Gupta, K. J. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2013; Open Access; Titel: The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco
This invention relates to the production and use of recombinant Pseudomonas-derived toxins modified to increase their toxicity and potency in therapy. More particularly, the invention relates to certain deletions in domain II of the amino acid sequence of Pseudomonas exotoxin the domain which relates to the toxins natural proteolytic processing.
The enzyme is involved in production of the rare amino acid 3-methylarginine, which is used by the epiphytic bacterium Pseudomonas syringae pv. syringae as an antibiotic against the related pathogenic species Pseudomonas syringae pv. glycinea ...
Coloured scanning electron micrograph (SEM) of Pseudomonas putida, Gram-negative, aerobic, enteric, rod prokaryote. Pseudomonas putida is a ubiquitous soil bacterium. Strains of P. putida have the ability to degrade organic solvents or hydrocarbons. This strain was isolated from a soil environment that had high levels of caffeine. It is known to biodegrade caffeine. Thus different strains of Pseudomonas putida can be used for bioremediation. Magnification: x1,600 when shortest axis printed at 25 millimetres. - Stock Image C032/1985
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1-Naphthol 2-hydroxylase (1-NH) which catalyzes the conversion of 1-naphthol to 1,2-dihydroxynaphthalene was purified to homogeneity from carbaryl-degrading Pseudomonas sp. strain C6. The enzyme was found to be a homodimer with subunit molecular weight of 66 kDa. UV, visible and fluorescence spectral properties, identification of flavin moiety by HPLC as FAD, and reconstitution of apoenzyme by FAD suggest that enzyme is FAD-dependent. 1-NH accepts electron from NADH as well as NADPH. Besides 1-naphthol (K (m), 9.1 mu M), the enzyme also accepts 5-amino 1-naphthol (K (m), 6.4 mu M) and 4-chloro 1-naphthol (K (m), 2.3 mu M) as substrates. Enzyme showed substrate inhibition phenomenon at high concentration of 1-naphthol (K (i), 283 mu M). Stoichiometric consumption of oxygen and NADH, and biochemical properties suggest that 1-NH belongs to FAD containing external flavomonooxygenase group of oxido-reductase class of enzymes. Based on biochemical and kinetic properties, 1-NH from Pseudomonas sp. ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Magnesium atom in PDB 3k4i: Crystal Structure of Uncharacterized Protein PSPTO_3204 From Pseudomonas Syringae Pv. Tomato Str. DC3000
Phenotypic and Genotypic Characterization of Extended Spectrum β-Lactamase Klebsiella pneumoniae and Fluorescent Pseudomonas spp. Strains from Market Garden Products and Their Watering Water in Benin West Africa. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
HERNANDEZ-MONTIEL, Luis G et al. Efficiency of two inoculation methods of Pseudomonas putida on growth and yield of tomato plants. J. Soil Sci. Plant Nutr. [online]. 2017, vol.17, n.4, pp.1003-1012. ISSN 0718-9516. http://dx.doi.org/10.4067/S0718-95162017000400012.. The objective of this study was to determine the efficiency of applying microcapsules and liquid inoculation of three Pseudomonas putida strains on growth and yield of tomato plants in greenhouse where the results showed differences between both treatments. Rhizobacterial strainsFA-8, FA-56, and FA-60 of P. putida, were assessed individually and combined to determine their capacity to produce indoleacetic acid (IAA). The three strains demonstrated the capacity to produce IAAin vitro, of which FA-56 stood out with 23.02 µg mL-1 in the microcapsule treatment with significant increases in plant height, stem diameter, radical volume, dry biomass, fruit yield, and rhizobacterial population (CFU). These responses could have been ...
26/01/2012. The Public Health Agency has today published leaflets for parents and carers providing information on preventing infections caused by Pseudomonas and screening patients for Pseudomonas. A fact sheet on is also available. To view these documents please follow the links below: Screening patients for Pseudomonas - Parent/Carer Information Preventing infections caused by Pseudomonas - Parent/Carer Information. Pseudomonas Fact Sheet. ...
Cluster VII was the largest cluster and contained 24 genes repressed in response to bean leaf extract and apoplastic fluid (Figure 3). Thirteen of these genes are known or hypothesized to be associated with pyoverdine production. This group includes pvdS, an extracytoplasmic sigma factor (ECF) needed for the transcription of genes for pyoverdine synthesis, a ferripyoverdine receptor (FpvA) involved in binding of iron-siderophore complexes in P. aeruginosa and a gene encoding a sigma-70 family protein with 34% identity (aa) to sigma factor FpvI, which is needed for fpvA transcription [56, 57]. In this cluster there are also five genes associated with biosynthesis of achromobactin and yersiniabactin, the secondary siderophores in P. syringae pv. syringae B728a and P. syringae pv. tomato DC3000 respectively (Table 2) [58, 59]. Two of these genes whose products belong to an ABC transporter system are located close to genes for yersiniabactin synthesis on the chromosome and are probably involved in ...
Pseudomonas sp. strain DY1 was newly isolated from soil with rotten wood and identified as a member of the genus Pseudomonas based on 16S rDNA and biochemical tests. Acid Black 172, a water soluble Cr-complex dye, was then selected as a model dye to investigate the decolorisation ability of the strain. It was observed that the growth of the strain was not inhibited by high dose of metal ions (10 mM), and efficient decolorisation was still observed when high concentrations of Fe2+, Fe3+ and Ca2+ existed. The optimal decolorising conditions obtained from Taguchi design were as follows: temperature 37˚C, pH 7.0, Fe3+ and proline concentrations of 7 mM and 3.0 g/L, respectively. Under the optimal conditions, 94.5% of Acid Black 172 (100 mg/L) could be decolorised by the strain in 24 h. The kinetics of the decolorisation best fitted the first order kinetic model (R2=0.981). Besides, the phytotoxicity study demonstrated a good detoxification by the strain. This work has a certain practical value in ...
The plant genus Capsium (Solanaceae) is of interest given its use in a number of herbal remedies by indigenous inhabitants throughout the world and the ability to synthesize the antibacterial metabolite and the active ingredient capsaicin (8-methyl-N-vanillyl-6-nonenamide) which is predominantly stored in the fruit of the plant (1, 2). To gain some insights into bacteria that associate with the Capsicum spp., we embarked on a project as a laboratory exercise in the Fundamentals of Plant Biochemistry and Pathology course in the Biotechnology and Molecular Bioscience Program at the Rochester Institute of Technology to assess the bacterial diversity of bacteria associate with the fruits of Capsicum annuum (birds eye chili) and Capsicum chinense (yellow lantern chili). This resulted in the isolation and identification of Bacillus safensis RIT372 and Pseudomonas oryzihabitans RIT370 from surface sterilized internal tissue from the fruits that were purchased from a farmers market in Rochester, New ...
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We report kinetic data for the two-step electron transfer (ET) oxidation and reduction of the two-domain di-heme redox protein Pseudomonas stutzeri cytochrome (cyt) c(4) by [Co(bipy)(3)](2- 3-) (bipy = 2,2-bipyridine). Following earlier reports, the data accord with both bi- and tri-exponential kinetics. A complete kinetic scheme includes both "cooperative" intermolecular ET between each heme group and the external reaction partner, and intramolecular ET between the two heme groups. A now data analysis scheme shows unequivocally that two-ET oxidation and reduction of P. stutzeri cyt c(4) is entirely dominated by intermolecular ET between the heme groups and the external reaction partner in the ms time range, with virtually no contribution from intramolecular interheme ET in this time range. This is in striking contrast to two-ET electrochemical oxidation or reduction of P. stutzeri cyt c(4) for which fast, ms to sub-ms intramolecular interheme ET is a crucial step. The rate constant dependence ...
Fingerprint Dive into the research topics of Inhibitor studies of dissimilative Fe(III) reduction by Pseudomonas sp. strain 200 (Pseudomonas ferrireductans). Together they form a unique fingerprint. ...
Can anybody tell me about the use of pseudomonas cepacia against nematodes and/or fungi. Does it work, are there already commercial products, how does it work ...
1JVZ: Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity
Weimberg, R. and Doudoroff, M. (1955). "The oxidation of L-arabinose by Pseudomonas saccharophila". J. Biol. Chem. 217: 607-624. PMID 13271422. ...
1. The ring-fission of cis- and trans-acenaphthene-1,2-diol by rat liver microsomes was studied. 2. 1,8-Naphthalic acid was detected and isolated after microsomal incubations of the diols. 3. The accompanying reduction of NAD+ was followed spectrophotometrically. 4. The optimum pH for the microsomal reaction was 9·4 for the oxidation of the cis-diol and 9·8 for that of the trans-diol. 5. p-Chloromercuribenzoate and 2,4-dichlorophenol inhibited the reaction. 6. Possible mechanisms for the microsomal ring-fission, involving 1,8-naphthalic aldehyde, are discussed.. ...
If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. Thank you!. Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016 ...
Response surface methodology for the optimization of chlorpyrifos-degrading conditions by Pseudomonas stutzeri ZH-1 Feng HE 1, Mi-mi ZHANG 2, Li-hong
Duque, E., Molina-Henares, A.J., De La Torre, J., Molina-Henares, M.A., Del Castillo, T., Lam, J., and Ramos, J.L. (2007a) Towards a genome-wide mutant library of Pseudomonas strain KT2440. In Pseudomonas: A Model System in Biology. Vol. V. Ramos, J.L., and Filloux, A. (eds). Dorchester, the Netherlands: Springer, pp: 227-251 ...
title:A Study of Metallo-Beta-Lactatamase Producing Pseudomonas Aeruginosa in Clinical Samples of S.S.G. Hospital. Author:Mehul S Chaudhari Tanuja B Javadekar Govind Ninama Neelam Pandya Jivraj Damor. Keywords:metallo-beta-lactatamase, producing pseudomonas aeruginosa, drug resistence. Type:Original Article. Abstract:Introduction: Pseudomonas spp. is common pathogen causing nosocomial infection. Acquired drug resistance is frequent in nosocomial isolates of Pseudomonas spp. Acquired metallo-?-lactamases (MBL) in pseudomonas spp. have recently emerged as one of the most worrisome resistance mechanism because of their capacity to hydrolyze all beta-lactam antibiotics including penicillins, cephalosporins and carbapenems, with the exception of aztreonam. Aim: To detect metallo-?-lactamase producing isolates of Pseudomonas aerugenosa from various clinical samples from patients admitted in our hospital. Material and methods : In this studyt we studied the prevalence, following standard methods of ...
Froese, R. and D. Pauly. Editors. (2018). FishBase. Hippocampus marginalis Kaup, 1856. Accessed through: World Register of Marine Species at: http://www.marinespecies.org/aphia.php?p=taxdetails&id=306804 on 2018-02-26 ...
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|568181348|ref|YP_008953893.1| from Pseudomonas monteilii SB3078. Plus protein sequence and external database links.
Here is a film we made in the lab to illustrate freezing induced by adding a drop of a suspension of Pseudomonas syringae to water supercooled to -6°C. Please
TY - JOUR. T1 - Adaptive divergence in experimental populations of Pseudomonas fluorescens. III. Mutational origins of wrinkly spreader diversity. AU - Bantinaki, Eleni. AU - Kassen, Rees. AU - Knight, Christopher G.. AU - Robinson, Zena. AU - Spiers, Andrew J.. AU - Rainey, Paul B.. PY - 2007/5. Y1 - 2007/5. N2 - Understanding the connections among genotype, phenotype, and fitness through evolutionary time is a central goal of evolutionary genetics. Wrinkly spreader (WS) genotypes evolve repeatedly in model Pseudomonas populations and show substantial morphological and fitness differences. Previous work identified genes contributing to the evolutionary success of WS, in particular the di-guanylate cyclase response regulator, WspR. Here we scrutinize the Wsp signal transduction pathway of which WspR is the primary output component. The pathway has the hallmarks of a chemosensory pathway and genetic analyses show that regulation and function of Wsp is analogous to the Che chemotaxis pathway from ...
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Pseudomonas bacteria can immobilise microalgae within moments. Researchers from Jena identified orfamid A as the chemical culprit.
PASADENA, Calif. (AP) A Los Angeles-area hospital said that some of its patients contracted an antibiotic-resistant "superbug" that has been linked to a type of medical scope and infected dozens of people around the country.. Huntington Memorial Hospital said in a statement Wednesday that it notified public health authorities after several patients who had procedures using Olympus Corp. duodenoscopes were found to have the resistant pseudomonas bacteria.. The hospital said it has quarantined the scopes while it investigates whether they may be linked to the infections.. The statement made no mention of the total number of infected patients or their conditions.. However, the Los Angeles Times said (http://lat.ms/1J5p3pl ) the problem was discovered in June and three patient infections have been reported to health officials.. Drug-resistant bacterial infections around the country have been linked to contamination of the reusable scopes, which are used for a procedure known as endoscopic retrograde ...
Ratio of pyoverdines and dihydropyoverdines produced by Pseudomonas putida. BTP16 is influenced by the growth rate of the strain ...
Sensitivity of Pseudomonas putida mutants to heat stress. Overnight-grown cells equivalent to an OD600 unit of 0.6 were mixed with LB broth in a total volume of
CURRENT RESEARCH. We work on the evolution of microbes, in particular ones associated with human disease. Currently our lab focuses on bacteria in the genus Pseudomonas from human homes and from the people and pets living in them. We combine studies of the distributions of these organism, studies of their patterns of phenotypic variability, and investigations of the genetic underpinnings of these phenotypes, and the evolutionary pressures that help mold them.. We have sampled human homes for many years, looking for members of the genus Pseudomonas. We have found that recovery of these bacteria varies among houses, among types of places within houses, as well as among seasons. We have also found that the opportunistic pathogen Pseudomonas aeruginosa is found most frequently in drains, rather than being widespread around the home. This is important because it suggests that for susceptible people, such as immunocompromised people or people with cystic fibrosis, controlling exposure to P. aeruginosa ...
Pseudomonas i urin Pseudomonas aeruginosa in Healthcare Settings | HAI | CDC. Behandla inte asymtomatisk bakteriuri hos äldre och urinodla endast vid symtom från urinvägarna
is a nickname given to a variant of the common bacterium Pseudomonas syringae ( P. syringae ). This strain of P. syringae lacks the ability to produce a certain surface protein, usually found on wild type ice plus P. syringae . The ice plus…
Principal Investigator:NAKAZAWA Teruko, Project Period (FY):1991 - 1992, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:細菌学