Fingerprint Dive into the research topics of Plasma levels of platelet-derived growth factor BB and transforming growth factor in patients with failed hip prostheses. Together they form a unique fingerprint. ...

In the present pre-clinical study, we used a long-acting vector (AAV) as a means to ensure long-term efficacy of transduction. Moreover, we sought to overcome the weakness of VEGF-A with respect to inducing vessel maturation and collateral growth by combining it with PDGF-B, a factor well-known for its role in mural cell recruitment. In a peripheral artery occlusion model, we found that the VEGF-A/PDGF-B combination did not alter the capillary density induced by VEGF-A alone (Figs. 1A and 1B). However, pericyte coverage of capillaries as well as growth of the conductance vessels (collaterals) increased significantly after PDGF-B cotransduction (Figs. 1C, 1D, 2A, and 2B), enabling a gain in limb perfusion. Combined VEGF-A/PDGF-B gene therapy was also found capable of improving the function of hibernating myocardium in a large-animal model (pigs) of chronic ischemia, in contrast to VEGF-A alone, even though the latter was given at a 5-fold higher dose (1 × 1013virus particles vs. 2 × 1012in the ...

TY - JOUR. T1 - Nicotine reorganizes cytoskeleton of vascular endothelial cell through platelet-derived factor BB. AU - Corvino, Valentina. AU - Cucina, A.. AU - Sapienza, P.. AU - Borrelli, V.. AU - Foresi, G.. AU - Randone, B.. AU - Cavallaro, A.. AU - Santoro DAngelo, L.. PY - 2000. Y1 - 2000. N2 - Background. Cigarette smoking has been directly linked to atherosclerosis formation and vascular graft failures but the role of nicotine in these processes is not yet completely understood. We investigated the release of platelet-derived growth factor BB (PDGF BB) by the bovine aortic endothelial cell (EC) after nicotine administration at concentrations similar to those found in plasma of active and passive smokers and the role of PDGF BB, autocrinally released, in EC cytoskeletal modification. Methods. EC were stimulated in a serum-free medium for 72 h with (2)-nicotine (from 6 3 1024 to 6 3 1028 M). The release of PDGF BB was assessed by inhibition antibody-binding assay and confirmed by Western ...

Effects of murrayafoline A on the PDGF-BB-stimulated activation of PDGF-Rβ, PLCγ1, Akt, ERK1/2, and STAT3. Quiescent VSMCs cultured in serum-free medium were

Background: Fibrosis may be considered the hallmark of systemic sclerosis (SSc), the end stage triggered by different pathological events. Transforming growth factor-β (TGF-β) and platelet-derived growth factor BB (PDGF-BB) are profibrotic molecules modulating myofibroblast differentiation and proliferation, respectively. There is evidence linking CD248 with these two molecules, both highly expressed in patients with SSc, and suggesting that CD248 may be a therapeutic target for several diseases. The aim of this work was to evaluate the expression of CD248 in SSc skin and its ability to modulate SSc fibrotic process. Methods: After ethical approval was obtained, skin biopsies were collected from 20 patients with SSc and 10 healthy control subjects (HC). CD248 expression was investigated in the skin, as well as in bone marrow mesenchymal stem cells (MSCs) treated with TGF-β or PDGF-BB, by immunofluorescence, qRT-PCR, and Western blotting. Finally, in SSc-MSCs, the CD248 gene was silenced by ...

Overexpression of platelet-derived growth factor-B increases the growth, invasion, and angiogenesis of gastric carcinoma cells through protein kinase B

The presence of tumor-associated macrophages (TAM) has been linked to increased tumor grade and poor clinical outcome in glioblastoma multiforme (GBM), suggesting that depletion or inhibition of these cells may suppress tumor growth. Colony-stimulating factor 1 receptor (CSF1R) is required for macrophage differentiation and survival, prompting Pyonteck and colleagues to investigate the therapeutic potential of CSF1R blockade in preclinical models of GBM. Treatment with BLZ945, a brain-penetrant, selective CSF1R inhibitor, specifically reduced macrophage proliferation and survival in vitro without affecting glioma cell viability. Furthermore, in a transgenic mouse model of proneural GBM driven by expression of the platelet-derived growth factor B (PDGFB) oncogene, CSF1R blockade reduced the malignant progression of GBMs and induced tumor regression in mice bearing established high-grade gliomas, resulting in prolonged survival. In addition, CSF1R inhibition impaired the intracranial growth of ...

Bioaim Human PDGF-BB EasyTest™ ELISA Kit suitable for Plasma, Serum in human. Reliably quantify 1pg/ml of PDGF-BB. It takes 1.5 hours.

REGRANEX Gel is the first and only FDA-approved recombinant platelet-derived growth factor (PDGF) therapy for diabetic neuropathic ulcers.

BioAssay record AID 421037 submitted by ChEMBL: Inhibition of PDGF-BB-stimulated Rac1 activity in human aortic smooth muscle cells assessed as reduction of ratio of Rac1GTP/Rac1 levels at 25 uM after 4 hrs by pull-down assay.

In the present study, we report that SMC migration is lower in response to the CM of ECs exposed to high SS compared with low SS. The increase in SS from 5 to 15 dyne/cm2 enhanced PDGF-BB and PDGF-AA secretion. In addition, the degree of phosphorylation of PDGFRα and PDGFRβ in SMCs was enhanced when the cells were incubated with CM from ECs exposed to low SS, and it was increased even further when the cells were incubated with CM from ECs exposed to high SS. Inhibition of PDGFRα-mediated effects either by overexpressing DN-PDGFRα or by a PDGF-AA antibody enhanced SMC migration in response to CMSS15; in contrast, these interventions failed to modulate SMC migration when CMSS5 was used as a chemoattractant. Taken together, these results indicate that PDGFRα activation on SMCs is enhanced when the cells are treated with the CM of ECs exposed to high SS and that, via this mechanism, PDGFRβ-directed SMC migration is inhibited. PDGF-BB binds ββ-, αβ-, and αα-receptor dimers, whereas ...

During limb development, dorsal and ventral muscles progressively separate to form individual muscles in a process called muscle splitting. In their study on p. 2579, Duprez and colleagues reveal that blood vessels regulate this process. They report that the location of endothelial cells, which are present in the developing chick limb before muscle, determines the site of future zones of muscle cleavage. By overexpressing VEGFA (a key growth factor in blood vessel development) in chick wing buds prior to muscle splitting, the researchers induced both blood vessel and connective tissue formation, while inhibiting muscle formation. Conversely, blocking blood vessel formation with a soluble VEGFR1 in chick wing buds caused muscle fusion. The authors propose that PDGFB (platelet-derived growth factor B), which is expressed in endothelial cells, is the molecular signal that regulates this process, perhaps by promoting the production of the extracellular matrix and attracting connective tissue cells ...

We have demonstrated 15- to 20-fold increases, relative to normal individuals, in the expression of PDGF-A and PDGF-B genes (reflected in mRNA levels) in circulating monocytes of HL and FH patients. These measurements of PDGF mRNA concentration were performed after isolation of mononuclear cells by density gradient centrifugation of blood, but with no further incubation. Although these are not strictly direct in vivo measurements, the very clear differences in PDGF mRNA observed between hypercholesterolemic and normocholesterolemic individuals argue strongly that the mononuclear cell isolation procedure has not distorted the data and that they reflect real differences in vivo. Northern blotting techniques could not detect PDGF-A or PDGF-B mRNA in freshly isolated, unstimulated monocytes,4 5 but PDGF-B mRNA was detectable after adherence of monocytes to a plastic surface.13 Bacterial lipopolysaccharide, interferon-γ, and phorbol esters have been variously shown to stimulate PDGF-A and/or PDGF-B ...

CSL will consider requests to share Individual Patient Data (IPD) from systematic review groups or bona-fide researchers. For information on the process and requirements for submitting a voluntary data sharing request for IPD, please contact CSL at [email protected].. Applicable country specific privacy and other laws and regulations will be considered and may prevent sharing of IPD.. If the request is approved and the researcher has executed an appropriate data sharing agreement, IPD that has been appropriately anonymized will be available. ...

Abstract of Paper: THE EFFECT OF HYPERGLYCEMIA ON SERUM TRANSFORMING GROWTH FACTOR-B1 (TGF-B1) LEVEL AND THEIR RELATIONS TO DIABETIC NEPHROPATHY IN PATIENTS WITH CONTROLLED TYPE 2 DIABETES MELLITUS , Author: Medhat Abdel Monem*, Awad EI-Abd*, Mosad Odah*, Ashraf Talaat** and Inas Abdel Monem* , Year: 2005 , Faculty of Medicine, Benha University

PDGF-BB Rat Recombinant produced in E.coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide of two B chains containing 2x109 amino acids (218 amino acids in total) and having a molecular mass of 24.4 kDa.The PDGF-BB is purified by proprietary chromatographic techniques.

TY - JOUR. T1 - Glucose alters platelet-derived growth factor-BB activity in human aortic vascular smooth muscle cells by stimulating protein phosphatase 2A in a protein kinase C-beta II-dependent pathway. AU - Campbell, Malcolm. AU - Trimble, Elizabeth. PY - 2004/9. Y1 - 2004/9. M3 - Article. VL - 47. SP - A445-A445. JO - Diabetologia. JF - Diabetologia. SN - 0012-186X. ER - ...

DOI: 10.11607/prd.00.0863 This preclinical study evaluated the efficacy of purified recombinant human platelet-derived growth factor (rhPDGF-BB), combined with a novel equine hydroxyapatite and collagen (eHAC) bone block, in providing vertical bone regeneration in critical-size defects simulating localized mandibular alveolar bone atrophy. In addition, the impact of barrier membrane placement in growth factor mediated bone regeneration was also studied. Bilateral posterior mandibular defects simulating severe localized bony atrophy were created in 12 adult foxhounds following removal of all four mandibular premolars. Three months later, the defects were grafted as follows: group A: eHAC block alone; group B: eHAC block + collagen membrane; group C: eHAC block + rhPDGF-BB; group D: eHAC block + rhPDGF-BB + membrane. The animals were sacrificed after 5 months and the grafted areas were examined histologically, radiographically, and clinically. Groups A and B (controls) exhibited little to no ...

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Non-gastrointestinal stromal tumor soft-tissue sarcomas (non-GIST STSs) constitute a heterogeneous group of tumors with poor prognosis. Fibroblast growth factor 2 (FGF2) and fibroblast growth factor receptor-1 (FGFR-1), in close interplay with platelet-derived growth factor-B (PDGF-B) and vascular endothelial growth factor receptor-3 (VEGFR-3), are strongly involved in angiogenesis. This study investigates the prognostic impact of FGF2 and FGFR-1 and explores the impact of their co-expression with PDGF-B and VEGFR-3 in widely resected tumors from non-GIST STS patients. Tumor samples from 108 non-GIST STS patients were obtained and tissue microarrays were constructed for each specimen. Immunohistochemistry was used to evaluate the expressions of FGF-2, FGFR-1, PDGF-B and VEGFR-3. In the multivariate analysis, high expression of FGF2 (P = 0.024, HR = 2.2, 95% CI 1.1-4.4) and the co-expressions of FGF2 & PDGF-B (overall; P = 0.007, intermediate; P = 0.013, HR = 3.6, 95% CI = 1.3-9.7, high; P = ...

Increased fetal hemoglobin (HbF) in b-globin gene disorders ameliorates the clinical symptoms of the underlying disease. 5-azacytidine, butyrate and hydroxyurea, have been shown to activate g-globin gene expression. It has also been found that hematopoietic growth factors can influence expression of g-globin in erythroid cultures and in animal models. This study was designed to evaluate the in vitro effects of the stem cell factor (SCF) and transforming growth factor-b (TGF-b) on g-globin gene reactivation of erythroid precursors derived from CD133+ cells in vitro. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis showed increased expression of the g-globin transcript in cell culture groups containing either TGF- b or SCF or both as compared to control (2.2-, 2.7- and 5.5-fold, respectively) (p|0.01). Production of HbF in a differentiated population was demonstrated using flow cytometry. The results of this study suggest that SCF and TGF-b warrant further evaluation as potential

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Objective: Platelet-derived growth factor-BB (PDGF)-induced intracellular signaling is involved in phenotypic modulation of vascular smooth muscle (VSM). This study has examined the PDGF-induced Ca2+ increase and the resultant effect on signaling pathways in proliferative compared with fully differentiated VSM. Methods: PDGF-induced changes in Ca2+ were measured in portal vein (PV) myocytes from 2-4-day-old (proliferating), compared to 6-week-old (differentiated), Sprague Dawley rats. Phospholipase C (PLC)g expression and activation of extracellular signal-regulated kinase (ERK) 1/2 was determined by immunoblotting or confocal immunolabelling. Activation of the Ca2+-dependent transcription factor, nuclear factor of activated T-cells (NFATc), was assessed by electromobility shift assay. Results: PDGF increased the intracellular Ca2+ concentration in differentiated, but not in proliferating, PV myocytes. This is probably due to very low expression of PLCg in proliferating PV. In 6-week-old PV, ...

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids ...

Amino Acid Sequence, Animals, Base Sequence, Blotting; Northern, Blotting; Western, Cell Movement/physiology, Chemotaxis/*physiology, Collagen Type I/*metabolism, Comparative Study, DNA Primers, DNA; Complementary/genetics, Electrophoresis, Fibroblasts, Gene Expression Regulation; Developmental/*physiology, Immunohistochemistry, In Situ Hybridization, Integrin alpha Chains/*genetics, Integrins/genetics/*metabolism, Mice/*genetics/metabolism, Molecular Sequence Data, Platelet-Derived Growth Factor/*metabolism, Precipitin Tests, Receptors; Collagen/genetics/*metabolism, Research Support; Non-U.S. Govt, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis; DNA ...

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Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. It is made up of two polypeptide chains (A and B) combined in three disulfide-linked dimeric forms (AA, AB, and BB). Here, the biosynthesis and proteolytic processing of the two homodimeric forms of PDGF (AA and BB) were studied in CHO cells stably transfected with A-chain (short splice version) or B-chain cDNA. PDGF-AA was processed to a 30-kD molecule which was secreted from the cells. In contrast, PDGF-BB formed two structurally distinct end products; a minor secreted 30-kD form and a major cell-associated 24-kD form. Immunocytochemical studies at light- and electron-microscopical levels revealed presence of PDGF in the Golgi complex, in lysosomes, and to a smaller extent in the ER. From analysis of cells treated with brefeldin A, an inhibitor of ER to Golgi transport, it was concluded that dimerization occurs in the ER, whereas the proteolytic processing of PDGF-AA and PDGF-BB precursors normally occurs in a ...

The Atul Bedi Lab in the Head and Neck cancer research program provides fundamental insights into the molecular determinants and mechanisms by which tumor cells evade death signals entrained by the immune system and anticancer agents. Their recent studies show that tumor-induced immune tolerance limits the in vivo anti-tumor efficacy of tumor-targeted antibodies and that the tumor cell-autonomous expression of transforming growth factor-b (TGF-b) is a key molecular determinant of the de novo or acquired resistance of cancers to EGFR-targeted antibody. Their laboratory has developed novel bi-functional antibody-based strategies to simultaneously counteract immune tolerance in the tumor microenvironment and to enhance the anti-tumor efficacy of targeted antibody therapies for the treatment of cancer.. Research Areas: targeted antibody therapies, transforming growth factor-b, cancer, head and neck cancer, tumor-targeted antibodies ...

TY - JOUR. T1 - Tumor necrosis factor-b in human pregnancy and labor. AU - Laham, N.. AU - Van Dunne, F.. AU - Abraham, Lawrence. AU - Farrugia, W.. AU - Bendtzen, K.. AU - Brennecke, S.P.. AU - Rice, G.E.. PY - 1997. Y1 - 1997. M3 - Article. VL - 33. SP - 53. EP - 69. JO - Journal of Reproductive Immunology. JF - Journal of Reproductive Immunology. SN - 0165-0378. ER - ...

We conducted a double-blind, randomized, placebo-controlled phase I/IIa study at two clinical centers in Sweden. Twelve patients with moderate PD received rhPDGF-BB via an implanted drug infusion pump and an investigational i.c.v. catheter. Patients were assigned to a dose cohort (0.2, 1.5, or 5 μg rhPDGF-BB per day) and then randomized to active treatment or placebo (3:1) for a 12-day treatment period. The primary objective was to assess safety and tolerability of i.c.v.-delivered rhPDGF-BB. Secondary outcome assessments included several clinical rating scales and changes in DAT binding. The follow-up period was 85 days.. ...

Seung-Hoon Lee, Yong-Suk Cho, Chan-sub Shim, Jung-joo Choi, Sun-Hee Oh, Jung-whan Kim, Wei Zhang and Jeho Lee. Aberrant Expression of Smad4 Results in Resistance against the Growth Inhibitory Effect of Transforming Growth Factor-b (TGF-b) in the SiHa Human Cervical Carcinoma Cell Lines. Int. J. Cancer,94:500-507 (2001 ...

Seung-Hoon Lee, Yong-Suk Cho, Chan-sub Shim, Jung-joo Choi, Sun-Hee Oh, Jung-whan Kim, Wei Zhang and Jeho Lee. Aberrant Expression of Smad4 Results in Resistance against the Growth Inhibitory Effect of Transforming Growth Factor-b (TGF-b) in the SiHa Human Cervical Carcinoma Cell Lines. Int. J. Cancer,94:500-507 (2001) ...

Blood-brain barrier (BBB) disruption and neuroinflammation are considered key mechanisms of pathogenic Escherichia coli invasion of the brain. However, the specific molecules involved in meningitic E. coli-induced BBB breakdown and neuroinflammatory response remain unclear. Our previous RNA-sequencing data from human brain microvascular endothelial cells (hBMECs) revealed two important host factors: platelet-derived growth factor-B (PDGF-B) and intercellular adhesion molecule-1 (ICAM-1), which were significantly upregulated in hBMECs after meningitic E. coli infection. Whether and how PDGF-B and ICAM-1 contribute to the development of E. coli meningitis are still unclear. The western blot, real-time PCR, enzyme-linked immunosorbent assay, immunohistochemistry, and immunofluorescence were applied to verify the significant induction of PDGF-B and ICAM-1 by meningitic E. coli in vivo and in vitro. Evans blue assay and electric cell-substrate impedance sensing assay were combined to identify the effects of

In normal adult physiology, intestinal smooth muscle cells (ISMC) are characterized as contractile and non-proliferative. Inflammation induces permanent changes to the intestine including hypertrophy of the smooth muscle layer largely due to smooth muscle cell (SMC) proliferation. While the consequences of this hyperplasia are largely unknown, increased muscularis mass may present permanent challenges to organ motility. Similar SMC hyperplasia is observed in other inflammatory pathologies including atherosclerosis and pulmonary arterial hypertension (PAH) where SMC de-differentiate into a synthetic phenotype and the mitogens responsible for hyperplasia have been well studied. However, there are limited investigations of SMC mitogens in intestinal inflammation. The identification of these factors may be of critical importance in the case of intestinal strictures, whereby recurring inflammation can lead to bowel obstruction requiring surgical intervention. A novel, primary rat ISMC model was ...

In normal adult physiology, intestinal smooth muscle cells (ISMC) are characterized as contractile and non-proliferative. Inflammation induces permanent changes to the intestine including hypertrophy of the smooth muscle layer largely due to smooth muscle cell (SMC) proliferation. While the consequences of this hyperplasia are largely unknown, increased muscularis mass may present permanent challenges to organ motility. Similar SMC hyperplasia is observed in other inflammatory pathologies including atherosclerosis and pulmonary arterial hypertension (PAH) where SMC de-differentiate into a synthetic phenotype and the mitogens responsible for hyperplasia have been well studied. However, there are limited investigations of SMC mitogens in intestinal inflammation. The identification of these factors may be of critical importance in the case of intestinal strictures, whereby recurring inflammation can lead to bowel obstruction requiring surgical intervention. A novel, primary rat ISMC model was ...

Neurofibromin 2 (NF2), a potent tumor suppressor, is reported to inhibit proliferation in several cell types. The role of NF2 in neointima hyperplasia after vascular injury is unknown. We explored the role of NF2 in proliferation, migration of vascular smooth muscle cell (VSMC) and neointima hyperplasia after vascular injury. NF2 phosphorylation was elevated in VSMC subjected to platelet-derived growth factor (PDGF)-BB and in artery subjected to vascular injury. Mice deficient for Nf2 in VSMC showed enhanced neointima hyperplasia after injury, increased proliferation and migration of VSMC after PDGF-BB treatment. Mechanistically, we observed increased nuclear p-NF2, declined p-Yes-Associated Protein (YAP), nuclear translocation of YAP after PDGF-BB treatment or injury. NF2 knockdown or YAP overexpression showed similar phenotype in VSMC proliferation, migration and neointima hyperplasia. YAP inhibition abolished the above effects mediated by NF2 knockdown. Finally, NF2 knockdown further promoted

BioAssay record AID 161259 submitted by ChEMBL: Inhibitory concentration against platelet-derived growth factor receptor beta phosphorylation in CHO cells.

Purpose. Graves ophthalmopathy (GO) is characterized by the infiltration of immune cells into the orbit, a process in which cytokines play a central role. Orbital fibroblasts are potent producers of cytokines on different stimuli. Recently, the authors showed increased expression of the PDGF-B chain in GO orbital tissue. The dimeric PDGF-BB molecule has been described to activate the NF-κB pathway, which is well recognized for its role in regulating cytokine production. This study was conducted to determine the role of PDGF-BB in the production of proinflammatory cytokines by orbital fibroblasts in GO. Methods. Orbital, lung, and skin fibroblasts were stimulated with PDGF-BB, and cytokine (IL-1β, IL-6, IL-8, IL-16, CCL2, CCL5, CCL7, TNF-α) production was measured by ELISA. Involvement of NF-κB activation through PDGF signaling was investigated by electrophoretic mobility shift assay, specific NF-κB inhibitors, and the PDGF-receptor kinase inhibitor imatinib mesylate. Results. IL-6, IL-8, ...

In this work we have evaluated the capacity of bone morphogenetic protein-2 (BMP-2) and fibrin-binding platelet-derived growth factor-BB (PDGF-BB) to support cell growth and induce bone regeneration using two different imaging technologies to improve the understanding of structural and organizational processes participating in tissue repair. Human mesenchymal stem cells from adipose tissue (hAMSCs) expressing two luciferase genes, one under the control of the cytomegalovirus (CMV) promoter and the other under the control of a tissue-specific promoter (osteocalcin or platelet endothelial cell adhesion molecule), were seeded in fibrin matrices containing BMP-2 and fibrin-binding PDGF-BB, and further implanted intramuscularly or in a mouse calvarial defect. Then, cell growth and bone regeneration were monitored by bioluminescence imaging (BLI) to analyze the evolution of target gene expression, indicative of cell differentiation towards the osteoblastic and endothelial lineages. Non-invasive ...

Migration of medial smooth muscle cells (SMCs) and their proliferation in the intima contribute to thickening of injured and atherosclerotic vessels. These events have been proposed to be regulated in part by platelet-derived growth factor (PDGF). Two separate PDGF receptors have been identified, PDGF-R alpha and PDGF-R beta. To study the functions of PDGF-R alpha and PDGF-R beta in vascular SMCs, neutralizing monoclonal antibodies (mAbs) specific for each of the two receptors were used. These antibodies allowed us to evaluate the role of each receptor for PDGF-induced proliferation and migration of cultured baboon SMCs. Both PDGF-AA and PDGF-BB stimulated SMC growth, with PDGF-BB being more potent than PDGF-AA. Studies with anti-PDGF-R alpha and anti-PDGF-R beta mAbs revealed that both PDGF receptors promoted the stimulatory signals for proliferation. In contrast, PDGF-BB stimulated SMC migration, whereas PDGF-AA had no stimulatory activity on its own. Additionally, PDGF-AA was able to suppress ...

Animals, Chemotaxis/*drug effects, GTP-Binding Proteins/metabolism, Genes; src/physiology, Helminth Proteins/metabolism, Humans, Isoenzymes/metabolism, Mice, Phospholipase C/metabolism, Phosphotransferases (Alcohol Group Acceptor)/metabolism, Platelet-Derived Growth Factor/*pharmacology, Protein-Tyrosine Kinase/metabolism, Proteins/metabolism, Receptors; Platelet-Derived Growth Factor/metabolism, Signal Transduction ...

Multi-centre randomized clinical trial on the efficacy and safety of recombinant human platelet-derived growth factor with β-tricalcium phosphate in human intra-osseous periodontal defects ...

Multi-centre, randomized clinical trial on the efficacy and safety of recombinant human platelet-derived growth factor with β-tricalcium phosphate in human intra-osseous periodontal defects. Journal of Clinical Periodontology 2011; 38(2), 163-172 ...

Tumor progression is a key aspect in oncology. Not even the overexpression of a powerful oncogenic stimulus such as platelet derived growth factor-B (PDGF-B) is sufficient per se to confer full malignancy to cells. In previous studies we showed that neural progenitors overexpressing PDGF-B need to undergo progression to acquire the capability to give rise to secondary tumor following transplant. By comparing the expression profile of PDGF-expressing cells before and after progression, we found that progressed tumors consistently downregulate the expression of the antiproliferative gene Btg2. We therefore tested whether the downregulation of Btg2 is sufficient and necessary for glioma progression with loss and gain of function experiments. Our results show that downregulation of Btg2 is not sufficient but is necessary for tumor progression since the re-introduction of Btg2 in fully progressed tumors dramatically impairs their gliomagenic potential. These results suggest an important role of Btg2 in

We conducted a double-blind, randomized, placebo-controlled phase I/IIa study at two clinical centers in Sweden. Twelve patients with moderate PD received rhPDGF-BB via an implanted drug infusion pump and an investigational i.c.v. catheter. Patients were assigned to a dose cohort (0.2, 1.5, or 5 μg rhPDGF-BB per day) and then randomized to active treatment or placebo (3:1) for a 12-day treatment period. The primary objective was to assess safety and tolerability of i.c.v.-delivered rhPDGF-BB. Secondary outcome assessments included several clinical rating scales and changes in DAT binding. The follow-up period was 85 days.. ...

V kolostre sa nachádzajú tieto rastové faktory: IGF-I (insulin-like GF I ) - rastový faktor podobný inzulínu (v najvyššej koncentrácií, aká je možná v prírode) TGF-B- (transforming growth factor B) EGF (Epitelial Growth factor) - faktor epidermálneho rastu FGF (Fibroblast growth factor) - fibroblastový rastový faktor PDGF (Platelet-derived GF) - faktor rastu krvných doštičiek. Tieto rastové faktory sú nevyhnutné pre správny vývoj zdravého organizmu. Podporujú správny rast svalov a kostí, chrupavky, pokožky, kolagénu a nervového tkaniva. Zvyšujú tvorbu vlastných imunoglobulínov a tým zvyšujú imunitu organizmu. Pomáhajú pri spaľovaní tukov. Regulujú hladinu cukru v krvi. Zlepšujú hojenie rán spôsobené popálením, mechanickým poškodením a urýchľujú hojenie herpesu na perách. Zlepšujú koncentráciu a pohotovosť mozgu. Dokážu dokonca u čerstvo prerušených nervov obnoviť ich zrastenie. Zabezpečujú dostatok energie pre organizmus, ...

Complete information for PDGFB gene (Protein Coding), Platelet Derived Growth Factor Subunit B, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

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We screened receptors and identified one, denoted earlier as ChemR23, that mediates RvE1 signal to attenuate nuclear factor-B. Specific binding of RvE1 to this receptor was confirmed using synthetic [3H]-labeled RvE1. Treatment of DCs with small interference RNA specific for ChemR23 sharply reduced RvE1 regulation of IL-12 ...

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Platelet-derived growth factor-stimulated actin rearrangement and edge ruffle formation have previously been shown to be dependent on activation of phosphatidylinositol 3′-kinase, the activity of which also is important for directed migration of cells. This lipid kinase binds to phosphorylated tyrosine residues Y740 and Y751 in the kinase insert of the human platelet-derived growth factor ss-receptor. We examined the role of two other tyrosine residues in the kinase insert of this receptor, Y775 and Y778, for ligand-induced actin rearrangement. Both were shown to be phosphorylation sites; Y775 was only marginally phosphorylated in cells expressing the wild-type ss-receptor, whereas Y778 was phosphorylated at higher stoichiometry. Mutant receptors Y775F, Y778F and Y775/778F were active kinases and mediated proliferative responses when expressed in porcine aortic endothelial cells. Fluorescence staining of actin in platelet-derived growth factor-stimulated PAE cells revealed that Y778 is ...

Looking for platelet-derived growth factors? Find out information about platelet-derived growth factors. see blood clotting blood clotting, process by which the blood coagulates to form solid masses, or clots. In minor injuries, small oval bodies called... Explanation of platelet-derived growth factors

The VHL protein (pVHL) is involved in the regulation of a protein known as hypoxia inducible factor 1α (HIF1α). This is a subunit of a heterodimeric transcription factor that at normal cellular oxygen levels is highly regulated. In normal physiological conditions, pVHL recognises and binds to HIF1α only when oxygen is present due to the post translational hydroxylation of 2 proline residues within the HIF1α protein. pVHL is an E3 ligase that ubiquitinates HIF1α and causes its degradation by the proteasome. In low oxygen conditions or in cases of VHL disease where the VHL gene is mutated, pVHL does not bind to HIF1α. This allows the subunit to dimerise with HIF1β and activate the transcription of a number of genes, including vascular endothelial growth factor, platelet-derived growth factor B, erythropoietin and genes involved in glucose uptake and metabolism.[12][13] A new novel missense mutation in VHL genes c.194 C,T, c.239 G,A, c.278 G,A, c.319 C,G, c.337 C,G leading to the following ...

Thrombomodulin (TM), a transmembrane glycoprotein highly expressed in endothelial cells (ECs), is a potent anticoagulant maintaining circulation homeostasis. Under inflammatory states, TM expression is drastically reduced in ECs while vascular smooth muscle cells (VSMCs) show a robust expression of TM. The functional role of TM in VSMCs remains elusive. We examined the role of TM in VSMCs activities in human aortic VSMCs stimulated with platelet-derived growth factor-BB (PDGF-BB). Using rat embryonic aorta-derived A7r5 VSMCs which do not express TM, the role of the chondroitin sulfate (CS) moiety of TM in VSMCs was delineated with cells expressing wild-type TM and the CS-devoid TM mutant. Expression of TM enhanced cell migration and adhesion/spreading onto type I collagen, but had no effect on cell proliferation. Knocking down TM with short hairpin RNA reduced PDGF-stimulated adhesion and migration of human aortic VSMCs. In A7r5 cells, TM-mediated cell adhesion was eradicated by pretreatment with

Platelet-derived growth factor (PDGF)-BB is a homodimeric growth factor with a broad range of target cells, notably mesoderm-derived cells, such as pericytes and mesangial cells, but also ectoderm-derived glial cells and neurons. PDGF-BB binds to two distinct receptor tyrosine kinases denoted PDGF receptor (PDGFR)- α and - β [1].. PDGF-BB and PDGFR-β are mainly expressed in the developing vasculature, where PDGF-BB is produced by endothelial cells which attract PDGFR-β expressing mural cells, i.e. pericytes and vascular smooth muscle cells [2-4]. Knockout studies of PDGF-B or PDGFR-β identified pericyte deficiency of the microvasculature as a main phenotype, resulting in lethal hemorrhage and edema at late gestation [5-7].. PDGF-BB is produced by most types of solid tumors, inducing stroma formation and neovascular response in order to secure sufficient supply of nutrients and oxygen [8]. Furthermore, it has been shown that PDGFR-β expression by pericytes is necessary for their recruitment ...

Apart from their endocrine functions renin-expressing cells play an important functional role as mural cells of the developing preglomerular arteriolar vessel tree in the kidney. The recruitment of renin-expressing cells from the mesenchyme to the vessel wall is not well understood. Assuming that it may follow more general lines of pericyte recruitment to endothelial tubes we have now investigated the relevance of the platelet-derived growth factor (PDGF)-B-PDGFR-β signaling pathway in this context. We studied renin expression in kidneys lacking PDGFR-β in these cells and in kidneys with reduced endothelial PDGF-B expression. We found that expression of renin in the kidneys under normal and stimulated conditions was not different from wild-type kidneys. As expected, PDGFR-β immunoreactivity was found in mesangial, adventitial and tubulo-interstitial cells but not in renin-expressing cells. These findings suggest that the PDGF-B-PDGFR-β signaling pathway is not essential for the recruitment ...

Linköping University, Faculty of Health Sciences. Linköping University, Department of Biomedicine and Surgery, Plastic Surgery, Hand Surgery and Burns. Östergötlands Läns Landsting, Reconstruction Centre, Department of Plastic Surgery, Hand surgery UHL. ...

MicroRNAs (miRNAs) and their role during tumor development have been studied in great detail during the last decade, albeit their expression pattern and regulation during normal development are however not so well established. Previous studies have shown that miRNAs are differentially expressed in solid human tumors. Platelet-derived growth factor (PDGF) signaling is known to be involved in normal development of the brain as well as in malignant primary brain tumors, gliomas, but the complete mechanism is still lacking. We decided to investigate the expression of the oncogenic miR-21 during normal mouse development and glioma, focusing on PDGF signaling as a potential regulator of miR-21. We generated mouse glioma using the RCAS/tv-a system for driving PDGF-BB expression in a cell-specific manner. Expression of miR-21 in mouse cell cultures and mouse brain were assessed using Northern blot analysis and in situ hybridization. Immunohistochemistry and Western blot analysis were used to investigate SOX2

This drug pipelines features 14 companies, including Samsung Bioepis Co Ltd, Shandong Boan Biological Technology Co Ltd, CSL Ltd, Alteogen Inc, Momenta Pharmaceuticals Inc

A PDGF Receptor that binds specifically to both PDGF-A chains and PDGF-B chains. It contains a Protein-Tyrosine Kinase activity that is involved in Signal Transduction ...

Supplementary MaterialsSupp Numbers1-S6. memory space cells was T-cell-intrinsic. Therefore, c-IAP E3 activity is required for 4-1BB co-receptor signaling and maintenance of CD8+ T-cell memory space. infection due to very high effector cytokine levels produced during the main effector response [27]. Using these mice, we have EMD534085 analyzed 4-1BB signaling and both the acute and memory space response to LCMV. We find that signaling downstream 4-1BB, and consequently the maintenance of a functional and effective pool of memory space T cells, requires c-IAP E3 activity. Results Impaired 4-1BB-induced signaling in c-IAP2H570A T cells In vitro studies have shown that engagement of 4-1BB on T cells induces the activation of the canonical NF-B pathway inside a c-IAP-dependent manner [18C20, 24]. We analyzed the part of c-IAP E3 activity in this process by taking advantage of mice in which endogenous c-IAP2 has been replaced with an E3-inactive point mutant, c-IAP2H570A, that also functions as ...

ウサギ・ポリクローナル抗体 ab62437 交差種: Ms,Hu 適用: WB,ELISA,IHC-P,ICC/IF…PDGF Receptor beta抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody…

Weinmann, Karolina (2017): Protektiver Effekt von PDGF-B in der Endotoxämie und in der polymikrobiellen Sepsis. Dissertation, LMU München: Faculty of Medicine ...

Expression of PDGFB (SIS, SSV) in cancer tissue. The cancer tissue page shows antibody staining of the protein in 20 different cancers.

PDGFR阻害剤（経路に合図することの標的を妨げる）がいろいろな分析のために使われて、いくつかは臨床試験に入りました。そして、それは新しいガン療法です。