To explore the effect of endogenous Ets-1 and Ets-2 on miR-126 expression, we altered the levels of endogenous Ets-1 and Ets-2 in endothelial cells. Endogenous Ets-1 and Ets-2 are expressed in endothelial cells and other cell types (Supplemental Figure III). To decrease endothelial Ets-1 and Ets-2 levels, we transfected HUVEC with siRNA directed to Ets-1, Ets-2, or control. siRNA to Ets-1 or Ets-2 decreased the expression of Ets-1 and Ets-2, respectively, in HUVEC (Figure 3C and 3D). Using this approach, we found that knockdown of Ets-2 decreased transactivation of the promoter construct in HUVEC (Figure 3E). Knockdown of Ets-1 also led to a smaller decrease in the transactivation of the Egfl7/miR-126 5′ reporter construct.. To explore the ability of Ets-1 and Ets-2 to alter the expression of miR-126 in endothelial cells, we transfected HUVEC with siRNA directed against Ets-1, Ets-2, or control and analyzed cellular RNA for miR-126 expression by qRT-PCR. Knockdown of Ets-1 decreased miR-126 ...
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BACKGROUND: The majority of prostate cancers harbor gene fusions of the 5-untranslated region of the androgen-regulated transmembrane protease serine 2 (TMPRSS2) promoter with erythroblast transformation-specific transcription factor family members. The common fusion between TMPRESS2 and v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG) is associated with a more aggressive clinical phenotype, implying the existence of a distinct subclass of prostate cancer defined by this fusion.. METHODS: We used complementary DNA-mediated annealing, selection, ligation, and extension to determine the expression profiles of 6144 transcriptionally informative genes in archived biopsy samples from 455 prostate cancer patients in the Swedish Watchful Waiting cohort (1987-1999) and the United States-based Physicians() Health Study cohort (1983-2003). A gene expression signature for prostate cancers with the TMPRSS2-ERG fusion was determined using partitioning and classification models and used in ...
ERG antibody [N3C3] (v-ets erythroblastosis virus E26 oncogene homolog (avian)) for WB. Anti-ERG pAb (GTX113079) is tested in Human, Mouse samples. 100% Ab-Assurance.
Modulation of the activity of proteins by phosphorylation has often been described as a binary switch, but Pufall et al. show that finer rheostat-like control can also be achieved. The transcription factor Ets-1 exhibits a graded DNA binding affinity that depends on the number of sites that are phosphorylated. Ets-1 exists in conformational equilibrium between a dynamic conformation that binds DNA and a well-folded inhibited state. Increasing phosphorylation progressively shifts the equilibrium toward the inhibited state and thus fine-tunes the level of activity. The phosphorylated region, which serves as the allosteric effector, is predominantly unstructured and flexible and probably acts through transient interactions.. M. A. Pufall, G. M. Lee, M. L. Nelson, H.-S. Kang, A. Velyvis, L. E. Kay, L. P. McIntosh, B. J. Graves, Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region. Science 309, 142-145 (2005). [Abstract] [Full Text]. ...
TY - JOUR. T1 - Etv5 regulates il-10 production in th cells. AU - Koh, Byunghee. AU - Hufford, Matthew M.. AU - Sun, Xin. AU - Kaplan, Mark. PY - 2017/3/1. Y1 - 2017/3/1. N2 - IL-10 is an immunoregulatory cytokine that has broad effects across the immune system. In Th cell subsets, Th2 cells produce considerable amounts of IL-10. The transcription factors that regulate IL-10 production in Th2 cells are still incompletely described. In this study, we demonstrate that the ETS family transcription factor ETS variant (Etv)5 regulates IL-10 production in Th2 cells. T cell-specific Etv5-deficient and littermate control mice demonstrated that IL-10 production and gene expression were significantly decreased in the absence of Etv5. In an Aspergillus fumigatus extract-induced inflammation model, IL-10-producing CD4+ T cells in bronchoalveolar lavage and lung were significantly decreased in mice that lacked Etv5 in T cells, compared with control mice. We showed that Etv5 directly binds to the Il10 locus ...
Germline mutations of transcription factors (e.g. PAX5, CEBPA, GATA2, RUNX1) have been associated with an inherited susceptibility to acute leukemia. Here we report 2 unrelated kindreds harboring germline mutations in ETV6, the gene encoding the transcription factor ETS variants 6. These kindreds were primarily characterized by thrombocytopenia and acute lymphoblastic leukemia (ALL). The first kindred, identified at MSKCC and HMC, includes 9 individuals with thrombocytopenia, and 3 individuals with pre-B ALL. Sequencing a subset of common and somatically altered leukemia genes in this family identified a rare heterozygous non-synonymous missense variation (T,C) in 6 family members with thrombocytopenia and 2 with ALL. Notably, this variant did not segregate in 9 individuals in the kindred without these phenotypes. The amino acid alteration is predicted to lead to an L349P substitution within the DNA binding domain of ETV6 (L349P, NPP_001978). In silico analyses using SIFT and Polyphen assigned ...
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Efficacy of ETS-1 transcriptional silencing. Expression level of ETS-1 in the Panc-1 cells was decreased after ETS-1 shRNA transfection. (A) Brightfield and flu
This novel protocol is designed to assess the neural bases of social interaction in infants. The paradigm is designed to tease apart...
Many animals sense environmental gases such as carbon dioxide and oxygen using specialized populations of gas-sensing neurons. The proper development and function of these neurons is critical for survival, as the inability to respond to changes in ambient carbon dioxide and oxygen levels can result in reduced neural activity and ultimately death. Despite the importance of gas-sensing neurons for survival, little is known about the developmental programs that underlie their formation. Here we identify the ETSfamily transcription factor ETS-5 as critical for the normal differentiation of the carbon dioxide-sensing BAG neurons in Caenorhabditis elegans. Whereas wild-type animals show acute behavioral avoidance of carbon dioxide, ets-5 mutant animals do not respond to carbon dioxide. The ets-5 gene is expressed in BAG neurons and is required for the normal expression of the BAG neuron gene battery. ets-5 may also autoregulate its expression in BAG neurons. ets-5 is not required for BAG neuron ...
In the spider Parasteatoda tepidariorum the transcription factor Ets4 is needed for cumulus integrity, dorsoventral patterning and for the activation of hunchback and twist expression.
ERG antibody, Internal (v-ets avian erythroblastosis virus E26 oncogene homolog) for WB. Anti-ERG pAb (GTX77743) is tested in Human samples. 100% Ab-Assurance.
Gene Information This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins such as apoptosis antigen Fas centromere protein C and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications including sumoylation phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2008]. ...
ETV6 (ETS-related protein Tel1, ETS translocation variant 6 , Tel) is an ETS family transcription factor. This protein contains two functional…
ETS-1, ETS-1A, ETS-1B, TNIP1, c-ets-1, TNFAIP3 interacting protein 1, c-ets-1 oncogene, p54 transcription factor, protein C-ets-1, transforming protein p54/c-ets-1, transforming protein p68/c-ets-1, virion-associated nuclear-shuttling protein, c-ets-1, Transforming protein p54, Transforming protein ...
ETS1兔多克隆抗体(ab124282)可与人样本反应并经WB, IP实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Heart development requires precise coordination of morphogenetic movements with cell fate specification and differentiation. Beh and colleagues have been investigating how this is achieved in ascidian embryos and now report that the forkhead transcription factor FoxF is essential for FGF-induced migration of heart precursor cells in Ciona intestinalis (see p. 3297). In ascidian embryos, FGF signalling, transduced via the MAPK pathway, activates the transcription factor Ets1/2, which is needed for heart tissue specification and cell migration. Beh et al. show that FoxF is rapidly activated in heart precursors in response to FGF signalling, identify the FoxF minimal heart enhancer, and show that Ets1/2 interacts with this in vivo. Expression of a dominant-negative form of FoxF in heart precursor cells, they report, inhibits their migration but not differentiation and results in the formation of an ectopic beating heart in the tail of juveniles. Overall, these results indicate that FoxF is a direct ...
Looking for online definition of E74-like factor 53 Epithelium-specific Ets transcription factor 2 in the Medical Dictionary? E74-like factor 53 Epithelium-specific Ets transcription factor 2 explanation free. What is E74-like factor 53 Epithelium-specific Ets transcription factor 2? Meaning of E74-like factor 53 Epithelium-specific Ets transcription factor 2 medical term. What does E74-like factor 53 Epithelium-specific Ets transcription factor 2 mean?
Sequence and functional properties of Ets genes in the model organism Drosophila.: Detailed molecular and genetic studies, coupled with the recent sequencing of
The non‐conservation in ETS proteins of the hydrophobic residues necessary for stabilization of the putative helix 1 as well as the insertion observed in that region for some ETS proteins, together with our secondary structure prediction, suggest that this region is unlikely to fold as an α‐helix. In the absence of experimental data about the structure adopted by this domain in either a monomeric or oligomeric state, its description as an HLH domain clearly appears to be premature.. The conserved fold of the amino‐terminal domain of ETS proteins is likely to underlie a conserved function. Our results show, however, that the conserved amino‐terminal domains of ETS‐1, ERG‐2 and GABPα are not homotypic oligomerization domains since they failed to replace the conserved amino‐terminal domain of TEL in inducing oligomerization when analyzed in an identical setting (Figure 6). These data are in accordance with other experimental approaches which have led to the conclusion that ETS‐1 ...
The ERK cascade regulates cellular proliferation, differentiation and survival, and is commonly activated in human cancers where it is associated with tumour aggression and metastases. In the present paper, we provide mechanistic and functional evidence of a regulatory pathway linking ERK signalling via inhibition of the transcriptional repressor capicúa to expression of the PEA3 Ets transcription factors that promote the transcription of pro-metastatic genes. Our findings could therefore explain why mRNA levels of ETV1 in particular are often elevated in breast, prostate, melanoma, gastrointestinal and other tumours, even when ETV1 overexpression is not driven by gene amplification or fusion to another gene [17,19,20,22-24,27]. In particular, our data are consistent with the identification of ETV1 and ETV5 in screens for mRNAs whose expression was decreased by BRAF(V600E)/MEK [MAPK (mitogen-activated protein kinase)/ERK kinase] inhibitors in melanoma cells [25,26]. It is also tempting to ...
Complete information for ELF3 gene (Protein Coding), E74 Like ETS Transcription Factor 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
InshaaALLAH Khamis ni seawal 9.00 pagi tiket bas Yui ke Putrajaya. Jujurnya, Yui jarang nak naik bas sebab lebih selesa dengan ETS tapi memandangkan member cakap kalau naik ETS kena ambil pengangkutan lain pula untuk ke Putrajaya ...
Human telomerase reverse transcriptase (hTERT) underlies cancer cell immortalization, and the expression of hTERT is regulated strictly at the gene transcription. Here, we report that transcription factor Ets2 is required for hTERT gene expression and breast cancer cell proliferation. Silencing Ets2 induces a decrease of hTERT gene expression and increase in human breast cancer cell death. Reconstitution with recombinant hTERT rescues the apoptosis induced by Ets2 depression. In vitro and in vivo analyses show that Ets2 binds to the EtsA and EtsB DNA motifs on the hTERT gene promoter. Mutation of either Ets2 binding site reduces the hTERT promoter transcriptional activity. Moreover, Ets2 forms a complex with c-Myc as demonstrated by co-immunoprecipitation and glutathione S-transferase pulldown assays. Immunological depletion of Ets2, or mutation of the EtsA DNA motif, disables c-Myc binding to the E-box, whereas removal of c-Myc or mutation of the E-box also compromises Ets2 binding to EtsA. ...
Complete information for ELK1 gene (Protein Coding), ELK1, ETS Transcription Factor, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
ETS2 contains an ETS DNA-binding domain and belongs to the ETS family. ETS2 is a target of protein kinase C and upregulates GM-CSF. Ets2 and its…
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Hematopoietic stem cells (HSCs) are essential for the maintenance of the hematopoietic system. However, these cells cannot be maintained or created in vitro, and very little is known about their generation during embryogenesis. Many transcription factors and signaling pathways play essential roles at various stages of HSC development. Members of the ETS (E twenty-six) family of transcription factors are recognized as key regulators within the gene regulatory networks governing hematopoiesis, including the ontogeny of HSCs. Remarkably, although all ETS transcription factors bind the same DNA consensus sequence and overlapping tissue expression is observed, individual ETS transcription factors play unique roles in the development of HSCs. Also, these transcription factors are recurrently used throughout development and their functions are context-dependent, increasing the challenge of studying their mechanism of action. Critically, ETS factors also play roles under pathological conditions, such as
This gene encodes an ETS family transcription factor. The product of this gene contains two functional domains: a N-terminal pointed (PNT) domain that is involved in the protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain. Gene knockout studies in mice suggest that it is required for hematopoiesis and maintenance of the developing vascular network. This gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma ...
Accumulation of specific proteins at synaptic structures is essential for synapse assembly and function, but mechanisms regulating local protein enrichment remain poorly understood. At the neuromuscular junction (NMJ), subsynaptic nuclei underlie motor axon terminals within extrafusal muscle fibers and are transcriptionally distinct from neighboring nuclei. In this study, we show that expression of the ETS transcription factor Erm is highly concentrated at subsynaptic nuclei, and its mutation in mice leads to severe downregulation of many genes with normally enriched subsynaptic expression. Erm mutant mice display an expansion of the muscle central domain in which acetylcholine receptor (AChR) clusters accumulate, show gradual fragmentation of AChR clusters, and exhibit symptoms of muscle weakness mimicking congenital myasthenic syndrome (CMS). Together, our findings define Erm as an upstream regulator of a transcriptional program selective to subsynaptic nuclei at the NMJ and underscore the importance
The data presented in this study show that Ets-1 is required for normal development and function of T reg cells and that defects in this cell subset were responsible for some of the immunological disorders in Ets-1−/− mice. Viable young mutant animals had reduced numbers of T reg cells in the spleen, but the frequency in the thymus appeared normal. In both sites, Ets-1−/− T reg cells had an unusual phenotype in that they expressed CD103 (Fig. 4 B), a marker typical of cells that experienced antigen under certain inflammatory conditions (Huehn et al., 2004; Suffia et al., 2005). This raised the possibility that the majority of thymic Ets-1−/− T reg cells were antigen-experienced recirculating cells, thereby masking an important quantitative deficit in thymic development of these cells. This was indeed the case, as supported by the very low frequency of thymic T reg cells in FTOCs (Fig. 4 C), in 5-d-old newborns (not depicted), and in mixed WT/KO chimeras analyzed at relative early ...
MEF, a recently identified member of the E74 family of ETS-related transcription factors, is a strong transcriptional activator of cytokine gene expression. Using a green fluorescent protein gene reporter plasmid regulated by an MEF-responsive promot
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Our findings clearly indicate that Spi-B is specifically required for development of human pDCs. These data add to observations that mice deficient for ICSBP/IRF-8 lack pDCs and CD8α+ DCs (13). Thus, both the ETS factor Spi-B and the IRF factor ICSBP/IRF-8 appear to be essential for pDC development. Interestingly, the ETS and IRF factors can cooperatively assemble on composite ETS-IRF DNA (EICE) elements, which were initially discovered in the immunoglobulin light chain enhancers but have later been found in promoters and enhancers of B lymphoid and myeloid genes (27). Similar to PU.1 and IRF-4, Spi-B and ICSBP/IRF-8 assemble in an ETS-IRF ternary complex of which the crystal structure was resolved recently (27). Given that both ICSBP/IRF-8 and Spi-B are required for pDC development, the structural data of Escalante et al. (27) make it very likely that Spi-B and ICSBP/IRF-8 cooperate in controlling pDC development.. Recent data have made clear that pDCs can develop both from Flt3+ lymphoid as ...