The non-lethal effects of the lymphocyte-derived pore-forming toxin perforin on the human erythroleukaemia cell line K562 were investigated. By using the fluorescent Ca2+ indicator fura-2, perforin was shown to cause intracellular Ca2+ concentration to rise transiently into the micromolar range in the absence of cell death. By fluorescence-activated cell sorting it was demonstrated that K562 cells took up the membrane-impermeant nuclear stain propidium iodide (PI) when exposed to non-lethal doses of perforin. The permeability to PI was short-lived, confirming the transience of the perforin pore. Analogies with non-lethal effects and recovery processes occurring in nucleated cells exposed to the membrane-attack complex of complement are drawn. ...

Given that the exocyst plays a role in spatially targeting vesicles to the plasma membrane (Heider and Munson, 2012), we also investigated whether the depletion of exocyst components had a differential effect on the repair of polarized (mechanical) and non-polarized (pore-forming toxin SLO) wounds. After SLO exposure, cells were incubated under conditions that were permissive (Ca2+) or non-permissive (no Ca2+) for plasma membrane repair, and exposed to the membrane-impermeant dye propidium iodide. Cells treated with control or Exo70-specific siRNA were equally capable of resealing their plasma membrane after SLO permeabilization in the presence of Ca2+, as indicated by the block in uptake (Fig. 2C, right panels). As expected, without Ca2+, permeabilized cells did not reseal, allowing propidium iodide influx (Fig. 2C, left panels). Flow cytometric quantification of propidium-iodide-positive cells confirmed that depletion of Exo70 (Fig. 2D) or Sec8 (Fig. 2E) did not affect the ability of the cells ...

Flow cytometer is a sophisticated and analytical tool which analyzes the phenotypic and functional characteristics of a cell in much lesser time. It measures the light scattering features, mainly forward scatter and side scatter properties of the cell. Optics, fluidics, and electronics are the main elements flow cytometer. Hydrodynamic focusing of the sheath fluid present in a sheath chamber enables the streamline motion of cells. This allows the encounter of each cell separately with the laser. A photodiode, photomultiplier tubes, optical filters, and beam splitters collect and emit the light or fluorescence in the form of signals. The signals are further processed by the electronic component and with the help of software, data are analyzed. For a cell to emit fluorescence, a number of fluorochromes (FITC, Alexa Fluor 488, Alexa Fluor 647 etc.) and dyes (propidium iodide, Hoechst, ethidium bromide etc.) are available. These fluorochromes may be conjugated with antibodies and emit fluorescence ...

Recent isolates of RX54-3 hybridoma cells (new cells) protect BALB/c mice against subsequent challenge with the tumorigenic myeloma parent cells used to construct this hybridoma. In contrast, hybridoma cells which have been maintained in tissue culture for long periods of time (old cells) are not protective. In the present study, we compared a number of properties of the new and old hybridoma cells and determined which line was more similar to the parent myeloma. We found that new hybridoma cells resembled myeloma cells in: (a) possessing A- and C-type viral particles on transmission electron microscopy and a relatively smooth surface on scanning electron microscopy; (b) being sensitive to a hypotonic solution containing the dye propidium iodide; (c) having similar DNA histograms on flow cytometric analysis; (d) being sensitive to the bacteriocin colicin HSC 10; and (e) being tumorigenic in nude mice. In contrast, old hybridoma cells differed in all of these characteristics from new hybridoma ...

Vitamin E prevents AICD. The day 6 prestimulated peripheral blood T cells were either left alone or pretreated with vitamin E (VE) and subsequently restimulated with α-CD3 (OKT3) in the absence or presence of CD95Fc or control human IgG1. Cell death was assessed after a further 48 hours by propidium iodide uptake. Results represent one experiment done in duplicates ...

流式細胞儀 細胞週期染色(flow cytometry propidium iodide stain) 在細胞凋亡的早期，發現有染色體濃縮 (chromosome condensation)的情況，

Propidium Iodide Buffer from BioLegend,Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.,biological,biology supply,biology supplies,biology product

Massimo Bonora is the author of this article in the Journal of Visualized Experiments: Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps

BioAssay record AID 611971 submitted by ChEMBL: Cell cycle arrest in human SW620 cells assessed as G1 phase delay at 5 uM after 24 to 48 hrs using propidium iodide staining by flow cytometry.

after fixation and perm, are you treating with RNAse A? P.I. will interculate into free RNA and make your cells light up like an xmas tree! If using P.I as a vital dye do not fix or perm, just add to media. Only dead cells will take up the dye, why live cells will expel the chemical ...

Tinción con anexina V y propidio yoduro (PI) ofrece a los investigadores una manera de identificar distintos tipos de muerte celular:...

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DNA, Human, Blood, Cell, Cell Lines, Cells, Interleukin, Interleukin-2, Nk Cell, Propidium, Propidium Iodide, Regulation, Screening, Therapeutic, Viruses, Consumption, Gastrointestinal Tract, Health, Health Benefit, Human Volunteers

p,Viability and GFP geometric mean fluorescent intensity (MFI) were assayed by flow cytometric analysis 48 h after transfection. For scFv-C4-PEST vs.scFv-C4: ,b,A,/b,. The percentage of propidium iodide (PI) positive nonviable cells is reduced. ,b,B,/b,. Total httex1-25Q-GFP and httex1-72Q-GFP reduction was confirmed. (means ± SEM; *** p,0.001 within groups.),/p ...

The Brutality Update to Dead Cells adds 25% more content to the game, including levels, abilities, weapons and enemies as well as re-working the upgrade system.

Legionella pneumophila is a ubiquitous microorganism widely distributed in aquatic environments and can cause Legionellosis in humans. A promising approach to detect viable cells in water samples involves the use of quantitative polymerase chain reaction (qPCR) in combination with photoactivatable DNA intercalator propidium monoazide (PMA). However, the PMA efficiency could be different depending on the experimental conditions used. The aim of this study was to compare two PMA exposure protocols: (A) directly on the membrane filter or (B) in liquid after filter washing. The overall PMA-induced qPCR means reductions in heat-killed L. pneumophila cells were 2.42 and 1.91 log units for exposure protocols A and B, respectively. A comparison between the results obtained reveals that filter exposure allows a higher PMA-qPCR signal reduction to be reached, mainly at low concentrations (p < 0.05). This confirms the potential use of this method to quantify L. pneumophila in water with low contamination.

Abstract. Abstract 2661Background:. Multiparameter flow cytometry (MFC) identifies mature T-cell neoplasms based on detection of populations with aberrant exp

JO and DP identified formalin fixed, paraffin wax embedded tissue blocks containing representative areas of tumour with the highest Gleason grade. Sections (5 μm thick) were mounted on to sialinised slides. The sections were dewaxed in xylene, washed twice in 100% ethanol, and air dried. They were then placed in the pretreatment solution of 30% sodium bisulphite (Sigma Aldrich, Poole, Dorset, UK) for 45 minutes at 45°C. Next, the slides were washed in 2× sodium chloride/sodium citrate (SSC; 0.3M sodium chloride, 0.03M sodium citrate at pH 7.0). Protein digestion was then carried out by immersing the slides in proteinase K (Sigma Aldrich) at 100 mg/litre for between 30 and 50 minutes at 45°C. The digestion time was calculated based on the degree of propidium iodide uptake, according to the manufacturers instructions (propidium iodide antifade; Quantum Appligene Lifescreen, Harefield, Middlesex, UK). After digestion, the slides were dehydrated in 70%, 90%, and 100% ethanol and then air dried. ...

Morphological changes in the macronucleus and micronuclei of the ciliated protozoon Heliophrya chapmani were investigated using the nucleic acid-specific stain propidium iodide. The fluorescence patterns of nuclei observed in propidium iodide prepara

Propidium iodide, an antitumor compound, was diffused into crystals of a complex between RNase A and deoxytetraadenylate (dpA)4). This complex has four deoxyoligomers bound per protein molecule. A difference Fourier analysis at 2.9 A showed that the principal binding site for the propidium in the crystals was a hydrophobic depression on the side of RNase away from the active site and apparently involves methionine 13 and phenylalanine 8. Binding of propidium at this site produces small conformational changes that effect binding of nucleotides at the active site of the enzyme. Fluorescence titrations in the presence and absence of nucleotide inhibitors suggested that propidium iodide is a competitive inhibitor of the enzyme with a Kl of approximately 1 mM. No significant binding of propidium to the 16 nucleotides of single-stranded DNA associated with each protein molecule was observed. ...

Immune nonresponder (INR) HIV-1-infected subjects are characterized by their inability to reconstitute the CD4+ T cell pool after antiretroviral therapy. This is linked to poor clinical outcome. Mechanisms underlying immune reconstitution failure are poorly understood, although, counterintuitively, INRs often have increased frequencies of circulating CD4+ T cells in the cell cycle. While cycling CD4+ T cells from healthy controls and HIV+ patients with restored CD4+ T cell numbers complete cell division in vitro, cycling CD4+ T cells from INRs do not. Here, we show that cells with the phenotype and transcriptional profile of Tregs were enriched among cycling cells in health and in HIV infection. Yet there were diminished frequencies and numbers of Tregs among cycling CD4+ T cells in INRs, and cycling CD4+ T cells from INR subjects displayed transcriptional profiles associated with the impaired development and maintenance of functional Tregs. Flow cytometric assessment of TGF-β activity ...

Immune nonresponder (INR) HIV-1-infected subjects are characterized by their inability to reconstitute the CD4+ T cell pool after antiretroviral therapy. This is linked to poor clinical outcome. Mechanisms underlying immune reconstitution failure are poorly understood, although, counterintuitively, INRs often have increased frequencies of circulating CD4+ T cells in the cell cycle. While cycling CD4+ T cells from healthy controls and HIV+ patients with restored CD4+ T cell numbers complete cell division in vitro, cycling CD4+ T cells from INRs do not. Here, we show that cells with the phenotype and transcriptional profile of Tregs were enriched among cycling cells in health and in HIV infection. Yet there were diminished frequencies and numbers of Tregs among cycling CD4+ T cells in INRs, and cycling CD4+ T cells from INR subjects displayed transcriptional profiles associated with the impaired development and maintenance of functional Tregs. Flow cytometric assessment of TGF-β activity ...

Mitochondria are an important source of superoxide production contributing to physiological and pathological responses, including vascular oxidative stress that is relevant to cardiovascular diseases

I have a killer toxin, which generates pores on susceptible yeast cells, so I want to make a viability test with PI. Now, I incubate the sensitive cells with the toxin for 12 and 24 hour, but the problem is that I cant afford to make the measurments with the flow cytometer at each sampling time. The question is that, after the sampling and staining, can the cells be stored at 4 C simply, or I have to fix them with 70% ethanol or formaldehyde (which one do you prefer?). Will not the PI diffused from the dead cells at 4 C overnight? I suppose it wont. What do you think ...

article{934d5e3b-3125-4a8e-9594-9c88d7695cb4, abstract = {Low-density polethylene disks with smooth or course surfaces were implanted in the abdominal wall of rats, and the tissue response was evaluated after 1, 6, or 12 weeks. Cell damage was detected by two different methods. Cells with increased membrane permeability could be identified using fluorescence microscopy by injection of propidium iodide prior to the killing of the rats. Second, cell death was verified by detection of DNA fragmentation. At 1 week a considerable number of the interfacial cells was stained with propidium iodide. Propidium-iodide-positive cells also were enriched at the edges of the disks irrespective of surface texture. The numbers of positive interfacial cells decreased markedly over time. Cells with DNA fragmentation initially displayed a scattered distribution; at later time points they appeared mainly in the outer portion of the enveloping capsule. The reactive capsule was thicker for the smooth surface, and ...

GelRed, GelGreen, EvaGreen - Fluorescence dyes, EtBr substitute Apoptosis and necrosis assay kitsCell biology accessory products Nucleic acid stains for microbiology Enzyme substrates Membrane and organelle stains Qubit® kits - DNA, RNA quantification Spectrophotometers a fluorometers Consumables GelRed Nucleic Acid Gel Stain, 10.000 x in water Biot41003 119 €144,30 € incl. Btw […]. ...

Vegetative cells growing in rich medium (Fig. 1 a and Video 1, available at http://www.jcb.org/cgi/content/full/jcb.200212104) were transferred to starvation medium SB containing 3 mM cAMP, in which they were incubated for 8 h. Control groups were then further incubated in SB alone, leading to a majority of cells with flat body and filopodia (Fig. 1 b and Video 2) that can survive as such for several days. In contrast, incubation in SB in the presence of DIF-1 led to the emergence within 8-16 h of very motile and strikingly compartmentalized cells that we call paddle cells (Fig. 1 c and Videos 3-7; see below). These stop moving within 15-26 h and change morphology to round cells (Fig. 1 d and Video 8). Round cells then progress toward massive vacuolization (Fig. 1 e and Videos 9-11) and still later to rupture of the cytoplasmic membrane (Video 12), which allows propidium iodide staining of the cell remnant (Cornillon et al., 1994). Incubation with propidium iodide leads to ,50% of the cells ...

Physiology Test Question - At the peak of the action potential, plasma membrane permeability to Na+ (pNa) is higher than the permeability to K+ (pK).

Dead Cells developer Motion Twin has just released a substantial new update for its roguelike platformer. The Corrupted Update adds a new challenge biome full of horrible blue birds to fight, and it makes key changes to Dead Cells' balance in order to keep things interesting.The new area is called The Corrupted Confinement, and it's accessible from the Toxic Sewers. From there, you'll go either to the Ancient Sewers or the Ramparts (via the Boss Cell level one door).

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Plants have always been a source of medicine and are still being used by traditional healers in the rural part of Africa, Asia and India to treat a range of illnesses including cancer. The in vitro anticancer activity of an 80 percent ethanol extract of Cyathula prostrata, an annual branching shrub used by traditional healers in Nigeria to treat cancer was investigated. No previous studies have outlined the possible pathways and mechanisms used by cancer cells when treated with C. prostrata. Dose response analysis was performed to determine the effective cytotoxic concentrations of C. prostrata on HeLa (cervical cancer cell line) and U937 (myelo-monocytic cell line). The IC50 values were 100.8 μg/ml and 64.4 μg/ml for HeLa and U937 cells, respectively. All further experiments were performed using 125 μg/ml C. prostrata extract and 50 μM cisplatin as positive control. With the use of the fluorescent DNA binding dye propidium iodide, the induction of tumour cell death by C. prostrata extract ...

microbiota, gut, infant, child, archaea, dairy products, milk, M. smithii, METHANOBREVIBACTER-SMITHII, METHANOMASSILIICOCCUS-LUMINYENSIS, PROPIDIUM MONOAZIDE, HUMAN FECES, SP NOV., METHANE, BACTERIAL, BREATH, MILK, ...

Results: Flow cytometric assessment of surface marker expression of mASCs demonstrated the following mean (SEM) percentage positive cells for the indicated surface antigens: CD44, 80.6 (5.3); CD90, 79.7 (4.6); and CD105, 26.7 (6.3). mASCs expressed the following mean (SEM) percentage negative cells for the indicated surface antigens: CD14, 96.4 (0.5); CD31, 97.6 (0.1); and CD45, 98.2 (0.1). There were no statistically significant differences in marker expression between mASC groups that underwent successful trilineage induction (n=8) and those that failed one differentiation pathway (n=6). mASCs from BRCA negative vs. BRCA mutation patients produced lesser quantities of triglycerides after two weeks of induction; average fold-change of lipid production in non-inherited cancer mASCs was 3.45±1.96 vs. 0.79±0.89 (p=0.03) (FIGURE 1). The mASC secretome of non-BRCA carriers vs. BRCA mutations differed in production of interleukin (IL) 1b (0.09 vs. 4.45-fold, p=0.03), IL10 (1.00 vs. 4.24-fold, ...

Antibiotic resistance is a serious threat to public health. The empiric use of the wrong antibiotic occurs due to urgency in treatment combined with slow, culture-based diagnostic techniques. Inappropriate antibiotic choice can promote the development of antibiotic resistance and can result in increased patient morbidity and mortality. We propose to use live/dead spectroscopy as a rapid alternative to culture-based techniques through application of the LIVE/DEAD® BacLightTM Bacterial Viability Kit. We have developed a spectroscopic device (Optrode) to measure fluorescence from SYTO 9 and propidium iodide (PI) stained cells that can be used to enumerate the bacterial load. We propose a procedure using the Optrode that will take bacteria in a clinical sample, challenge with a panel of antibiotics, and measure live/dead ratios to determine the best bactericidal choice. Initial investigations revealed that the experimental parameters outlined in the kit instructions do not generate results that ...

BioAssay record AID 609847 submitted by ChEMBL: Cell cycle arrest in human HeLaS3 cells assessed as accumulation at S phase at 150 uM after 48 hrs using propidium iodide staining by flow cytometry (Rvb = 19.62%).

Purpose : Previous our studies showed that the commercially available ophthalmic product, as fixed antibiotic combination (Colbiocin®), containing tetracycline, chloramphenicol and colistimethate sodium, had a good efficacy against Candida spp., including resistant strains (Marino et al., ARVO 2016). The aim of this work was to study the mode-of-action of this antibacterial product against yeasts. Methods : C. albicans ATCC and clinical isolated treated with sub-MIC concentrations of Colbiocin eye drop or with antibiotics of the formulation, alone and in combination each other, were examined by propidium iodide staning and MitoTracker red staining to determine cell permeability and mitochondrial function, respectively. Observations under the fluorescence microscope were performed. Results : Microscopic examination using propidium staining demonstrated that the C. albicans cells treated with colistimethate sodium were stained red because they lost in cell membrane integrity. Propidium iodide, a ...

PromoKine offers a wide range of dyes for fluorescently staining dead cells. The membrane-impermeable nuclear stains can also be used for fixed cells. Commonly used dyes for staining dead cells include; orange-fluorescent ethidium bromide (EB), red-fluorescent propidium iodide (PI), green-fluorescent acridine orange (AO) and blue-fluorescent DAPI. The toluidine derivative Trypan Blue is a classical non-fluorescent dye used to selectively stain dead cells and shows a distinctive blue colour under the microscope. ...

Original article can be found at: http://www.sciencedirect.com/science/journal/1385299X Copyright Elsevier B.V. DOI: 10.1016/S1385-299X(02)00201-5 [Full text of this article is not available in the UHRA ...

BioTek Anwendungshinweise, 04-Nov-14, Automated Hemocytometer-Based Live/Dead Cell Counting using Phase Contrast and Color Brightfield Imaging

BioTek Anwendungshinweise, 04-Nov-14, Automated Hemocytometer-Based Live/Dead Cell Counting using Phase Contrast and Color Brightfield Imaging

Assessment of cellular immunity in children with Down syndrome by determining the ratio of subpopulations of T lymphocytes CD3+, CD4+ and CD8+ in peripheral lood using flow cytometric method ...

Supplementary Materialsoncotarget-06-13520-s001. antibody restrained migration. Collectively, these total outcomes claim that c-Src regulates secreted protein, like the exosomal Cyr61, which get excited about modulating the metastatic potential of triple adverse breast tumor cells. 0.05). Oddly enough, shRNA-c-Src induction didnt alter the proliferation of adherent MDA-MB-231-Tet-On-shRNA-c-Src cells. The outcomes from metabolic activity (MTT) and cell viability (Trypan blue) assays (Components and Strategies) had been similar in charge and Doxy-treated cells (Shape 1C, D). It ought to be noted how the percentage Isoorientin of Trypan blue-stained cells was always smaller than 5% (data not shown), indicating that c-Src suppression was not cytotoxic. Furthermore, c-Src suppression did not alter expression of cyclin D1 and p27Kip1 (Figure ?(Figure1E).1E). Consistently, flow cytometric analysis of the cell cycle using propidium iodide labeling showed no differences in the percentage of cells in ...

Interphase alpha-tubulin immunostaining of human A549 epithelial lung cancer cells. The image shows vehicle-treated cells, which have a normal cytoplasmic tubulin distribution (green) and were well adherent. ALEXA 488 (green) was used to visualize the tubulin distribution; cell nuclei (red) were stained with propidium iodide. Cells treated with CYT997 for 1 hour had tubulin networks disrupted and had begun to dislodge. For details, see article by Burns and colleagues 3036. ...

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Curcumin is a major diarylheptanoid component of|i| Curcuma longa|/i| with traditional usage for anxiety and depression. It has been known for the anti-inflammatory, antistress, and neurotropic effects. Here we examined curcumin effect in neural plasticity and cell viability. 60-channel multielectrode array was applied on organotypic hippocampal slice cultures (OHSCs) to monitor the effect of 10 |i|μ|/i|M curcumin in long-term depression (LTD) through low-frequency stimulation (LFS) to the Schaffer collaterals and commissural pathways. Cell viability was assayed by propidium iodide uptake test in OHSCs. In addition, the influence of oral curcumin administration on rat behavior was assessed with the forced swim test (FST). Finally, protein expression levels of brain-derived neurotrophic factor (BDNF) and cyclooxygenase-2 (COX-2) were measured by Western blot in chronically stressed rats. Our results demonstrated that 10 |i|μ|/i|M curcumin attenuated LTD and reduced cell death.

TY - JOUR. T1 - Flow cytometric methods for the analysis of human basophil surface antigens and viability. AU - Bochner, Bruce S.. AU - McKelvey, Alicia A.. AU - Schleimer, Robert P.. AU - Hildreth, James. AU - MacGlashan, Donald W.. PY - 1989/12/20. Y1 - 1989/12/20. N2 - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.. AB - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low ...

This work reviews common flow cytometric methods and applications for the study of bacterial organisms. Flow cytometry is fluorescent method capable of both quantitative and qualitative analysis at the single cell level. It can offer insights about bacterial population dynamics, phenotypic heterogeneity and more. This work features a basic introduction to flow cytometry and presents some of the commonly measured variables, such as viability or membrane potential with an emphasis on the fluorescent probes used to visualize them. The difficulties of adapting flow cytometry to bacterial physiology are discussed, as well as the advantages and disadvantages of the particular probes and methods. Finally, this work seeks to demonstrate the flexibility as well as the shortcomings of flow cytometry using examples of practical applications in basic research, environmental microbiology, biotechnology, clinical practice. Keywords: flow cytometry, microbial subpopulations, fluorescent labelling, bacterial ...

Each day approximately forty to fifty billion (yes billion) cells die in every human. Have you ever wondered where these dead cells go and how your body eliminates the dead cells? Try getting rid of a small dust particle from your eye. You know just how difficult it is. Every day without you realizing it, you are elimination billions of cells without breaking a sweat. A significant part of these dead cells is destroyed, worn out, and regenerated in your skin. These cells slough off and new ones made in their place. Removal of dead cells from your skin is the simpler part of this process. Many cells die inside your body. Here is where it gets interesting.. Your cell has a membrane which keeps the contents within isolated from everything outside of it. When a cell dies, the membrane layer becomes leaky and allows the contents outside to mingle with the contents inside. Enzymes will now begin to digest the insides of the cell. White Blood Cells (neutrophils and macrophages) which are also ...

was used as negative control. After 48 h, cells were collected for total RNA and protein extraction.Cell Proliferation AssayCell proliferation was assessed by the cell counting kit-8 (CCK8) assay according to the manufacturers protocol (Dojindo Laboratories, Gaithersburg, MD, USA). Cells in a 96-well plate were incubated with CCK-8 solutions for 1 h at 37uC. Absorbance of each well was quantified at 450 nm by the Tecan Infinite 2000 Microplate Reader.Flow CytometryCell cycle analysis was performed using ethanol-fixed cells stained with propidium iodide in buffer containing RNaseA. The DNA content was assessed using a FACS Calibur Flow Cytometer (Becton-Dickinson, San Jose, CA, USA). Apoptotic cells were assessed following the manufacturers protocol (Becton-Dickinson). Briefly, treated cells were collected, washed twice with ice-coldSULT2B1b Promotes Hepatocarcinoma INCB-039110 web ProliferationFigure 5. Knock-down of SULT2B1b suppressed tumorigenesis in vivo. 1.56106 NC-GFP-LV or ...

Inhibition of PI3K in serum-starved LNCaP cells induces apoptosis that is abrogated by introduction of EGF or serum. LNCaP cells serum-starved for 20 h were treated with fresh medium containing 10% serum (serum), serum-free medium (No serum), medium containing 10% serum and 25 μ M LY (LY+serum), serum-free medium with 25 μ M LY (LY) or serum-free medium containing 25 μ M LY and 100 ng/ml EGF (LY+EGF) for 6, 12 and 20 h before (a) cell death was determined by propidium iodide staining and (c) caspase 3 and (d) caspase 9 activity as described in Materials and methods. Percentage of cells in sub-G1 was analysed using the WINMDI 2.8 software. DNA histograms depicting % cell in sub-G1 of one of the experiments after 20 h incubation in different treatments is shown in (b). Results shown in (a), (c) and (d) are a mean of three experiments performed in duplicates±s.e. ...

Tripos muelleri infected by Amoebophrya ceratii-like. Photo: Catharina Alves-de-Souza. Green: cytoplasm of the parasite stained by FISH. Red: nuclei stained by propidium iodide. Blue: Host theca stained by calcofluor. ...

This protocol was developed to utilize imaging flow cytometry (IFCM) in combination with fluorescent dyes to both enumerate and analyze morphological features of live and dead cells in a mixed live/dead bacterial sample

Fluorescent micrographs and quantitative analysis of CAOV-3 cells double-stained with acridine orange and propidium iodide. Cells were treated with liriodenine

An easy, accurate, high-throughput-suitable method for determining cell viability.. Catalog #CTL-LDC-100-2. Includes: CTL-LDC™ Reagent and fifty disposable, 2-chamber hemocytometer slides. A flat rate for shipping will be applied to all orders. To use your companys FedEx account number, please contact us before placing your order.. Pricing is for US and Canada only and does not include shipping. All others, contact the distributor in your region.. ...

Caudalie Deep Cleansing Exfoliator is suitable for exfoliating all types of skin by removing dead cells and allowing more a light skin. Learn more.