Complete 16S rRNA gene sequences of three representative strains of anaerobic, Gram-negative, pigmented, moderately saccharolytic, indole-positive bacteria isolated from the oral cavity of humans were determined. According to comparative analyses of the rRNA sequence data, this organism represents a previously unknown species within the genus Prevotella. In addition, 22 representative strains and 21 reference strains (including 11 Prevotella intermedia and 10 Prevotella nigrescens strains) were subjected to multilocus enzyme electrophoretic analysis. The strains were consistently separated into three clearly distinct groups, corresponding to their previous entities. On the basis of the present phylogenetic results that confirmed our biochemical and genetic data, we propose a new species, Prevotella pallens. The type strain is NCTC 13042 (= AHN 10371).
Prevotella heparinolytica ATCC ® 35895™ Designation: HEP TypeStrain=True Application: Produces heparin lyase heparinase
The nucleotide positions of the target site for the forward primer on T. bryantii 16S rRNA gene sequences were 380-400 while those of the reverse primer were 934-953, yielding a 575-bp PCR product. The primer set was designed to cover all rumen Treponema and named g-TrepoF. The online basic local alignment search tool (blast) program (http://blast.ncbi.nlm.nih.gov/Blast.cgi) was used to determine the specificity of the forward primer.. The specificity of the primers was further tested by PCR amplification using genomic DNA from pure cultures of 16 representative rumen bacterial strains including T. bryantii ATCC33254, F. succinogenes ATCC19169, Ruminococcus albus 8, Ruminococcus flavefaciens C94, Prevotella ruminicola 23, Prevotella bryantii B14, Prevotella brevis GA33, Butyrivibrio fibrisolvens. H17c, B. fibrisolvens D1, Eubacterium ruminantium Silmitasertib GA195, Selenomonas ruminantium GA192, Succinivibrio dextrinosolvens ATCC19716, Succinimonas amylolytica ATCC19206, Streptococcus bovis ...
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DNA from the batch culture fluid and rumen contents in the feeding study was extracted and purified using the repeated bead beating plus column method [19] with a DNA stool mini kit (QIAamp DNA Stool Mini Kit; Qiagen, Hilden, Germany). The purified DNA was quantified using a NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and diluted to 10 ng/μL before being subjected to polymerase chain reaction (PCR) amplification. The diluted DNA was used for quantitative real-time PCR (qPCR) to monitor the abundance of rumen representatives including total bacteria, total archaea, fungi, protozoa, Fibrobacter succinogenes (F. succinogenes), Ruminococcus albus (R. albus), Ruminococcus flavefaciens (R. flavefaciens), genus Prevotella, Prevotella bryantii, Prevotella ruminicola (P. ruminicola), Anaerovibrio lipolytica (A. lipolytica), and Butyrivibrio group. Details of qPCR such as primers, standards, PCR conditions, and calculations were as described by Koike et al [20] and Ohene-Adjei et al [21]. ...
ID D5EWU7_PRER2 Unreviewed; 354 AA. AC D5EWU7; DT 15-JUN-2010, integrated into UniProtKB/TrEMBL. DT 15-JUN-2010, sequence version 1. DT 07-JUN-2017, entry version 28. DE SubName: Full=RyR domain protein {ECO:0000313,EMBL:ADE82520.1}; GN OrderedLocusNames=PRU_0332 {ECO:0000313,EMBL:ADE82520.1}; OS Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23). OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; OC Prevotella. OX NCBI_TaxID=264731 {ECO:0000313,EMBL:ADE82520.1, ECO:0000313,Proteomes:UP000000927}; RN [1] {ECO:0000313,EMBL:ADE82520.1, ECO:0000313,Proteomes:UP000000927} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 19189 / JCM 8958 / 23 {ECO:0000313,Proteomes:UP000000927}; RX PubMed=20585943; DOI=10.1007/s00248-010-9692-8; RG North American Consortium for Rumen Bacteria; RA Purushe J., Fouts D.E., Morrison M., White B.A., Mackie R.I., RA Coutinho P.M., Henrissat B., Nelson K.E.; RT "Comparative genome analysis of Prevotella ruminicola and Prevotella ...
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T helper 17 (TH17) cells are key players in multiple sclerosis (MS), and studies in animal models demonstrated that effector TH17 cells that trigger brain autoimmunity originate in the intestine. We validate in humans the crucial role of the intestinal environment in promoting TH17 cell expansion in MS patients. We found that increased frequency of TH17 cells correlates with high disease activity and with specific alterations of the gut mucosa-associated microbiota in MS patients. By using 16S ribosomal RNA sequencing, we analyzed the microbiota isolated from small intestinal tissues and found that MS patients with high disease activity and increased intestinal TH17 cell frequency showed a higher Firmicutes/Bacteroidetes ratio, increased relative abundance of Streptococcus, and decreased Prevotella strains compared to healthy controls and MS patients with no disease activity. We demonstrated that the intestinal TH17 cell frequency is inversely related to the relative abundance of Prevotella ...
In rheumatoid arthritis (RA), immunological triggers at mucosal sites, such as the gut microbiota, may promote autoimmunity that affects joints. Here, we used discovery-based proteomics to detect HLA-DR-presented peptides in synovia or peripheral blood mononuclear cells and identified 2 autoantigens, N-acetylglucosamine-6-sulfatase (GNS) and filamin A (FLNA), as targets of T and B cell responses in 52% and 56% of RA patients, respectively. Both GNS and FLNA were highly expressed in synovia. GNS appeared to be citrullinated, and GNS antibody values correlated with anti-citrullinated protein antibody (ACPA) levels. FLNA did not show the same results. The HLA-DR-presented GNS peptide has marked sequence homology with epitopes from sulfatase proteins of the Prevotella sp. and Parabacteroides sp., whereas the HLA-DR-presented FLNA peptide has homology with epitopes from proteins of the Prevotella sp. and Butyricimonas sp., another gut commensal. Patients with T cell reactivity with each self-peptide ...
ID R6PT18_9BACT Unreviewed; 432 AA. AC R6PT18; DT 24-JUL-2013, integrated into UniProtKB/TrEMBL. DT 24-JUL-2013, sequence version 1. DT 22-NOV-2017, entry version 15. DE RecName: Full=M18 family aminopeptidase {ECO:0000256,RuleBase:RU004387}; DE EC=3.4.11.- {ECO:0000256,RuleBase:RU004387}; GN ORFNames=BN637_00431 {ECO:0000313,EMBL:CDC27508.1}; OS Prevotella sp. CAG:386. OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; OC Prevotella; environmental samples. OX NCBI_TaxID=1262925 {ECO:0000313,EMBL:CDC27508.1, ECO:0000313,Proteomes:UP000018046}; RN [1] {ECO:0000313,EMBL:CDC27508.1, ECO:0000313,Proteomes:UP000018046} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=MGS:386 {ECO:0000313,Proteomes:UP000018046}; RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., RA Sunagawa S., Plichta D., Gautier L., Le Chatelier E., Peletier E., RA Bonde I., Nielsen T., Manichanh C., Arumugam M., Batto J., RA Santos M.B.Q.D., Blom N., Borruel N., Burgdorf K.S., Boumezbeur ...
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Intestinal bacteria are linked to our diet. When participants in this last Danish study were divided by their level of intestinal bacteria, it was found that people with a high proportion of Prevotella bacteria compared to the bacteria Bacteroides lost 3.5 kg more in 26 weeks, they ate a diet composed of the principles of the "New Nordic Diet" compared to those who consumed a Danish average diet.. Subjects with a low proportion of Prevotella bacteria compared to Bacteroides did not lose any extra weight by following this new Nordic Diet. Overall, about 50% of the population has a high proportion of Prevotella bacteria compared to Bacteroides bacteria.. The study shows that only about half of the population will lose weight if it eats according to the Danish national dietary guidelines - and eats more fruits, vegetables, fiber and whole grains. The other half of the population does not seem to benefit from this change of regime. These people should focus on other feeding recommendations and ...
Dr. Kishore Bhat, Rajiv Gandhi University of Health Sciences, Bangalore.. 05Evaluation of role of pigmented and non-pigmented Prevotella species in patients with chronic periodontics and oral health: A culture based and molecular study. Dr Vijayalakshmi S. Kotrashetti Rajiv Gandhi University of Health Sciences, Bangalore ...
Abstract: Se evaluó la actividad de ampicilina, ampicilina-sulbactama, cefoxitina, ceftriaxona, imipenem, piperacilina, piperacilina-tazobactama, clindamicina, metronidazol y azitromicina frente a 166 cepas de bacterias anaerobias aisladas en 8 hospitales de Buenos Aires. Se estudiaron: Bacteroides grupo fragilis (65), Fusobacterium spp. (26), Prevotella spp. (21), Porphyromonas spp. (10), Clostridium difficile (10), otros clostridios (12) y cocos gram-positivos (22). Las CIMs se determinaron usando el método patrón de dilución en agar recomendado por el NCCLS, documento M11-A5. Los antibióticos más activos fueron metronidazol y piperacilina-tazobactama que exhibieron valores de CIM90£ 2 μg/ml y £ 4 μg/ml frente a los microorganismos gram-negativos y £ 2 μg/ml y £ 8 μg/ml frente a los microorganismos gram-positivos, respectivamente. Entre los b-lactámicos el orden de actividad frente a bacilos gram-negativos fue: imipenem > piperacilina > cefoxitina > ceftriaxona > ampicilina. ...
A few weeks ago (June 2012), a paper in Nature by a group of researchers suggested that despite the vast geographical and nutritional differences in the human population, that just three predominant bacterial clusters (referred to as enterotypes hereafter) could explain all of our gastric microbial mixes.[1] This they suggest indicates the existence of a limited number of well-balanced host-microbial symbiotic states that might respond differently to diet and drug intake.. Each of these three enterotypes are identifiable by the variation in the levels of one of three genera: Bacteroides (enterotype 1), Prevotella (enterotype 2) and Ruminococcus (enterotype 3). These enterotypes are not as sharply delimited as, for example, human blood groups; they are, in contrast, densely populated areas in a multidimensional space of community composition. They are nevertheless likely to characterise individuals, in line with previous reports that gut microbiota are quite stable in individuals and can even be ...
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We supply a selection of bacterial QC reference strains as well as a comprehensive range of superior quality bacterial growth media for Molecular Biological & Molecular Genetics applications.
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Real-time PCR using intercalating dyes (e.g., SYBR Green) or labeled probes is a highly reliable, standard tool to investigate the expression and regulation of genes. It offers reproducible characterization and quantification of biomolecules such as DNA, mRNA, miRNA or lncRNA. We offer a comprehensive range of quantitative (RT-)PCR tools from standard reverse transcription and qPCR kits to specially designed assays and arrays, so you can choose the best approach to suit your needs. Get instant success in gene expression analysis from any sample at any time with our extensive PCR solutions ...
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At JCST we are able to combining a comprehensive range of both classical wet chemistry and instrumental techniques to give the most cost effective solutions to most chemical problems.. Classical wet chemistry. The wet chemistry basically falls into two distinct areas. Using classic methods to identify chemical species. Using standard methods to isolate the chemical species under investigation and then identify the species and if necessary quantify the amount present. Identification can be achieved by a colour change or possibly pH. The amount present can be identified by the intensity of colour although this would normally be done instrumentally or by precipitation.. Using classical methods to prepare samples for analysis by instrumental methods. Samples of metals for analysis are often dissolved in acids to enable the resulting solution to be analysed using such techniques as Atomic Absorption/emission. In this way classical methods enable the samples to be analysed in the most effective ...
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INTERTRONICS supplies a comprehensive range of bonding and protective materials - together with the associated consumables and equipment - to customers in high technology, high performance assembly industries - including electronics, medical device, plastics, optical, automotive, energy, defence and aerospace. The company supplies over 3,000 regular customers, including multinational manufacturing giants, production facilities, specialist design and development businesses, universities, training organisations and government establishments.. adhere is INTERTRONICS brand which encompasses its bonding, coating, sealing, encapsulating, potting, masking and gasketing materials, together with the most appropriate equipment and accessories for dispensing and curing them.. Because adhere brings together this proven range of integrated products from selected and trusted partners, INTERTRONICS customers have the confidence that everything will work together efficiently and cost-effectively, underpinned ...
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OECD Ministers at their meeting in May 2013 agreed to take a comprehensive range of measures as set out in the OECD Action Plan for Youth. The first objective of these measures is to tackle the current situation of high youth unemployment and underemployment. The second objective is to produce better outcomes for youth in the longer run by equipping them with relevant skills and removing barriers to their employment.. ...
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