Corning™ Polystyrene Poly-D-Lysine Coated Microplates 384well; White/Clear bottom Corning™ Polystyrene Poly-D-Lysine Coated Microplates Coated...
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Our ViewPlate microplates are engineered for bottom reading systems and high content imaging. This high quality microplate can be utilized in your cell based assay for superior results.. Poly-lysine is a synthetic positively-charged polymer, existing as two enantiomers: Poly-D-lysine (PDL) and Poly-L-lysine (PLL). Adherence of certain cell types to poly-lysine-coated surfaces is based on the electrostatic interaction of the poly-D-lysine polycation with the negative charges of the cell membrane. Use of poly-lysine coatings on plate surfaces can help mediate the negative charges of the cell membrane and the negative charge of the surface. Both PDL and PLL are commonly used however PDL is not degraded by cellular proteases and is therefore often the preferred choice. As Poly-lysine is a synthetic protein, it does not influence the signaling pathways of the cells and is completely free of any animal contaminants. Almost all cell types will adhere to Poly-lysine coated plate bottoms.. Leveraging ...
Our ViewPlate microplates are engineered for bottom reading systems and high content imaging. Glass exhibits better optical properties and is perfectly flat, but is less-suited for cell culture. Glass therefore usually has to be coated with proteins that enhance cell adherence and growth (e.g., poly-D-lysine or collagen). This high quality microplate can be utilized in your cell based assay for superior results.. Poly-lysine is a synthetic positively-charged polymer, existing as two enantiomers: Poly-D-lysine (PDL) and Poly-L-lysine (PLL). Adherence of certain cell types to poly-lysine-coated surfaces is based on the electrostatic interaction of the poly-D-lysine polycation with the negative charges of the cell membrane. Use of poly-lysine coatings on plate surfaces can help mediate the negative charges of the cell membrane and the negative charge of the surface. Both PDL and PLL are commonly used however PDL is not degraded by cellular proteases and is therefore often the preferred choice. As ...
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Learn more about 96 Well Clear Flat Bottom Poly-D-Lysine Coated Microplate, Aseptic, Corning®. We enable science by offering product choice, services, process excellence and our people make it happen.
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Product Description:. Poly-L-lysine hydrobromide is a positively charged synthetic polyamino acid having one HBr per lysine unit. It is a crystalline solid soluble in water. Applications for poly-L-lysine hydrobromide include the promotion of cell adhesion to solid substrates for culture dishes or slides, the conjugation to active molecules for improved activities, the layer-by-layer deposition techniques, and the complexation with nucleic acids for gene expression.. For cell culture applications, poly-L-lysine hydrobromide with a molecular weight greater than 30,000 Da is recommended. The poly-L-lysine hydrobromide (MW=52,000 Da) is easier to use because it is less viscous in solution; however, the poly-L-lysine hydrobromide with an higher molecular weight (MW=84,000 Da) has more attachement sites per polymer chain available to the cells.. Our poly-L-lysine hydrobromide has been purified by dialysis, sterile-filtered on 0.2um, lyophilized, and stored under Ar.. Storage Conditions:. Store ...
Polyamino acids are able to adopt ordered conformations, such as α-helices and β-sheets, through cooperative hydrogen bonding. These conformations impart polyamino acids with various unique properties and functions in biological environments.
Read "A novel ultrasensitive electrochemiluminescence biosensor for glutathione detection based on poly-L-lysine as co-reactant and graphene-based poly(luminol/aniline) as nanoprobes, Biosensors and Bioelectronics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Gene Delivery with Hyperbranched Polylysine THÈSE N O 5216 (2011) PRÉSENTÉE le 4 novembre 2011 À LA FACULTÉ SCIENCES ET TECHNIQUES DE LINGÉNIEUR LABORATOIRE DES POLYMÈRES PROGRAMME DOCTORAL EN SCIENCE
Poly-D-lysine (PDL), a synthetic positively charged amino acid chain that alters surface charges on the culture substrate, is widely used as a coating to enhance cell adhesion and protein absorption. Poly-D-lysine (PDL), a synthetic positively charged amino acid chain that alters surface charges on the culture substrate, is widely used as a coating to enhance cell adhesion and protein absorption. Learn More ...
Poly-D-lysine (PDL), a synthetic positively charged amino acid chain that alters surface charges on the culture substrate, is widely used as a coating to enhance cell adhesion and protein absorption. Poly-D-lysine (PDL), a synthetic positively charged amino acid chain that alters surface charges on the culture substrate, is widely used as a coating to enhance cell adhesion and protein absorption. Learn More ...
The effect of polycations poly-L-arginine and major basic protein on L-14C-arginine uptake and LPS-induced IL-8 production by NCI-H292 cells. Parallel cultures
For these tests a fluorescence assay was used: a model peptide antigen of the sequence LFEAIEGFI (MHC Kd-restricted) was labelled with the fluorescent dye fluorescein isothiocyanate (FITC) in accordance with the manufacturers instructions (Molecular Probes). The uptake or binding of FITC-labelled peptide on its own ("pulsed") or together with various concentrations of basic amino acids (polylysine with a chain length of 16 to 490, polyarginine with a chain length of 15 to 720) by the MHC Kd-restricted monocyte macrophage cell line P388D1 was measured by throughflow cytometry. In order to do this, 1 x 106 P388D1 cells were incubated in a final volume of 1 ml of medium (DMEM/10% FCS) in a centrifugal test tube with 5 µg of FITC-labelled peptide on its own or with a mixture of peptide and polyamino acid for 30 minutes at 37°C and then washed thoroughly to eliminate any free peptide. The polyamino acids were added in a concencration of 50, 25, 12, 6 and 3 µg per ml of medium, containing 5 µg of ...
* found in: L-Tryptophan, Poly-L-Lysine, MW |=70,000, Poly-L-Lysine Hydrobromide, Poly-D-Lysine Hydrobromide from Synthetic, L-Glutamine, L-Glutamic..
How to coat 96 well plate with poly-L-Lysine for cell culture use? - posted in -Immunology and Neuroscience-: How to coat 96 well plate with Poly-L-lysine?
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Pharmaceutical composition containing at least one peptide or protein (fragment) with an immunomodulatory activity together with an adjuvant. The peptide is derived from a pathogenic agent or a tumour antigen. The adjuvant is capable of increasing the binding of the peptide to the cells of the individual to be treated or of increasing the entry of the peptide into the cells and strengthening the immunomodulatory activity of the peptide. Preferred adjuvants are basic polyamino acids such as polyarginine or polylysine, optionally conjugated with a cellular ligand such as a carbohydrate group or transferrin. The composition is particularly intended for use as a vaccine, e.g. as a tumour vaccine.
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The objective of our research is to develop a liposomal gene delivery system that specifically targets potent therapeutic genes to breast cancers which are positive for the human epidermal growth receptor 2 (Her-2). Current liposomal gene delivery systems predominately utilize cationic lipids, which efficiently bind and deliver plasmid DNA, but also result in nonspecific gene expression in the lungs and liver tissue. We attempt to improve on specificity, by using a two-component delivery system. The first component is a cationic polyethylene glycol (PEG) grafted polylysine which carries the plasmid DNA. The polylysine/DNA particle binds nonspecifically to cells but has limited ability to bypass the endosomal membrane. The second component is an anionic Her-2 targeting liposome which is disulfide bound to the pore forming protein Listeriolysin O (LLO). These liposomes bind with high selectivity to Her-2 positive cells and once internalized into the reducing conditions of an endosome, release LLO ...
Images: Polycaspase Assay Kit, green was used to assess cell death in primay rat hippocampal neurons.Cells were plated on 25-mm poly-l-lysine-coated coverslips at 300,000 cells per coverslip. Cells were used at 4 or 8 days in vitro. Composite imagae (A) 3 out of 4 cells are apoptotic (green). No cells were necrotic as both of the PI-positive cells were FLICA-positive; they had compromised membranes and were probably in the late stages of apoptosis rather than necrosis. (B) 3 Caspase-positive cells fluoresce green ...
Images: Polycaspase Assay Kit, green was used to assess cell death in primay rat hippocampal neurons.Cells were plated on 25-mm poly-l-lysine-coated coverslips at 300,000 cells per coverslip. Cells were used at 4 or 8 days in vitro. Composite imagae (A) 3 out of 4 cells are apoptotic (green). No cells were necrotic as both of the PI-positive cells were FLICA-positive; they had compromised membranes and were probably in the late stages of apoptosis rather than necrosis. (B) 3 Caspase-positive cells fluoresce green ...
Hi all,. I had a question about my transfections. I have the cells grown on coverslips 16 hours prior to transfection. After, I use lipofectamine to transfect my cells. At 24 hours post-transfection they look healthy and I change the media. After 48 hours, though, the cells are very streched out and starting to detach. The cells on the plastic that did not land on the coverslip, however, are just fine. Ive tried using fibronectin and poly-D-lysine and still I get the same problem.. Are there any other techniques that I can try? I can see that the transfection has worked when I try to image these cells, but theyre so unhealthy that I dont want to trust the data.. ...
Cell culture flasks , roller bottles , multilayer cell culture vessels, cell culture plates , inserts , cell culture tubes , cell culture dishes , Poly-D-Lysine , incubators, cryotubes , slides , ELISA plates ...
Cell culture flasks , roller bottles , multilayer cell culture vessels, cell culture plates , inserts , cell culture tubes , cell culture dishes , Poly-D-Lysine , incubators, cryotubes , slides , ELISA plates ...
Histidine containing reducible polycations based on CH(6)K(3)H(6)C monomers (His6 RPCs), are highly effective DNA transfection agents combining pH buffering endosomal escape mechanisms with rapid unpackaging following reduction in the cytoplasm. We examined their ability to mediate siRNA uptake into cells focusing on hepatocyte delivery. Co-delivery of EGFP siRNA with pEGFP plasmid DNA reduced reporter gene expression by 85%. However while DNA transfection efficiency increased with polymer size, with 162 k His6 RPCs proving the most effective, delivery of siRNA alone to EGFP stably expressing cells was only possible using 36-80 k polymers. Analysis of particle sizes showed that 80 k polymers formed more compact siRNA complexes than 162 k polymers. The reducible nature of the polymer was necessary for siRNA activity, since siRNA combined with non-reducible polylysine showed little activity. Incorporation of a targeting peptide from the Plasmodium falciparum circumsporozoite (CS) protein onto His6 RPCs,
Histidine containing reducible polycations based on CH(6)K(3)H(6)C monomers (His6 RPCs), are highly effective DNA transfection agents combining pH buffering endosomal escape mechanisms with rapid unpackaging following reduction in the cytoplasm. We examined their ability to mediate siRNA uptake into cells focusing on hepatocyte delivery. Co-delivery of EGFP siRNA with pEGFP plasmid DNA reduced reporter gene expression by 85%. However while DNA transfection efficiency increased with polymer size, with 162 k His6 RPCs proving the most effective, delivery of siRNA alone to EGFP stably expressing cells was only possible using 36-80 k polymers. Analysis of particle sizes showed that 80 k polymers formed more compact siRNA complexes than 162 k polymers. The reducible nature of the polymer was necessary for siRNA activity, since siRNA combined with non-reducible polylysine showed little activity. Incorporation of a targeting peptide from the Plasmodium falciparum circumsporozoite (CS) protein onto His6 RPCs,
R115777 was a potent inhibitor of isolated human FPT that was competitive for the CAAX peptide substrate. The molecule was selective for FPT versus PGGT I despite having no obvious structural relationships to the CAAX recognition motif, which is the basis of selectivity for these two similar enzymes (31 , 32) . Earlier leads in the chemical series confirmed that the imidazole group is the central pharmacophore prompting the hypothesis that the imidazole interacts with the coordination structure of the zinc catalytic site (33 , 34) . As with other inhibitors that are competitive for the peptide-binding site, R115777 exhibited a loss of potency with a polylysine containing K-RasB peptide substrate (15) .. To a degree, the loss of potency with K-RasB at the level of the enzyme was reflected in studies in intact cells in vitro. Cell lines with N-ras or H-ras mutations responded to lower concentrations of R115777 than did the cell lines bearing K-ras mutations. These findings are consistent with ...
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Synthetic polycations have been investigated widely as delivery vectors of nucleic acid therapeutics, such as plasmid DNA and siRNA. Polycations form complexes, called polyplexes, with the nucleic acids, thereby protecting them from degradation and facilitating transport across cellular membranes. Traditionally, polycations have been viewed as pharmacologically inert components of the delivery systems. We proposed a conceptually new approach that polycations can have both a delivery function as well as pharmacologic activity, to enhance the therapeutic outcome of gene and siRNA therapies. We developed a novel class of polycations that not only deliver nucleic acids but also function as antagonists of the CXCR4 chemokine receptor. Many preclinical and clinical studies have found significant correlation between CXCR4 overexpression and tumor growth and metastasis in many different types of cancer. CXCR4 expression is often associated with poor survival and aggressive type of cancer. We are working ...
Looking for online definition of benzylpenicilloyl polylysine in the Medical Dictionary? benzylpenicilloyl polylysine explanation free. What is benzylpenicilloyl polylysine? Meaning of benzylpenicilloyl polylysine medical term. What does benzylpenicilloyl polylysine mean?
Dear Janet, I can help you with problem #1 of your inquiry..... A) You need to be sure that you are using a high molecular weight poly-lysine (i.e. greater than 70kD. We routinely use 150-300kD poly-lysine Sigma P1399) If you use lower molecular weight poly-lysine the sections will float off of the slides. With the higher molecular weight stuff our sections remain attached throughout in situ hybridizations, a rather rigorous treatment for tissues. B) You need to be sure that your slides are completely clean before poly-lysine treatment. Packaged slides marked as pre-cleaned are no such thing! They have a thin coating of oil on them to prevent sticking together. I usually soak a box of slides in 70% ethanol + a drop or two of glacial acetic. I then remove the slides as needed and wipe them until dry with a Kimwipe. Hope that this is helpful. =============================================================================== Melissa Melan mmelan at lucy.wellelsey.edu Department of Biological ...
TY - JOUR. T1 - Steric stabilization of poly-L-lysine/DNA complexes by the covalent attachment of semitelechelic poly[N-(2-hydroxypropyl)methacrylamide]. AU - Oupicky, David. AU - Howard, Kenneth A.. AU - Koňák, Čestmír. AU - Dash, Philip R.. AU - Ulbrich, Karel. AU - Seymour, Leonard W.. PY - 2000/7/1. Y1 - 2000/7/1. N2 - The concept of steric stabilization was utilized for self-assembling polyelectrolyte poly-L-lysine/DNA (pLL/DNA) complexes using covalent attachment of semitelechelic poly[N-(2-hydroxypropyl)methacrylamide] (pHPMA). We have examined the effect of coating of the complexes with pHPMA on their physicochemical stability, phagocytic uptake in vitro, and biodistribution in vivo. The coated complexes showed stability against aggregation in 0.15 M NaCl and reduced binding of albumin, chosen as a model for the study of the interactions of the complexes with plasma proteins. The presence of coating pHPMA had no effect on the morphology of the complexes as shown by transmission ...
Week 4: Tingling and pricking particularly around the nipples due to an increased blood supply . Imported and distributed by PB Pretty White Co.. You may hesitate to apply moisturizer to acne-prone skin but a good moisturizer can actually help calm your skin and keep Tight-fitting hats can also cause or worsen acne especially if you sweat. issues like marks on the skin around the eyes and nose As you make to-do lists for this busy time of year below are some to-dos fo better skin. Scarabeus Funktions-Merino Hoody STARLIGHT - blue 16900 EUR inkl.. Find best value and selection for your Collagen TREATMENT Mask - ANTI AGING ELASTICITY- 3 PCS search on eBay. What can I do to stop them from getting worse? This lightweight oil-free sheer tinted moisturizer is good for all skin types - especially sensitive skin. -Poly-l-lysine (poly-lysine or PL) The two dark circles represent fully inhibitory concentration combinations of nisin and poly-lysine: And for patients seeking smoother skin Smoothbeam safely ...
PRIMARY OBJECTIVES:. I. To determine whether the immune response to cancer/testis antigen 1B (NY-ESO-1) elicited by vaccination with CDX-1401 (anti-DEC205-NY-ESO-1 fusion protein vaccine) plus polyinosinic-polycytidylic acid stabilized with poly-L-lysine and carboxymethylcellulose (poly-ICLC) is substantially increased by prior expansion in the number of circulating dendritic cells (DC) by therapy with CDX-301 (fms-related tyrosine kinase 3 ligand [Flt3L]) (recombinant flt3 ligand).. SECONDARY OBJECTIVES:. I. To assess the effect of the vaccine regimen on immune responses to other ongoing and nascent antitumor response antigens associated with melanoma (e.g., preferentially expressed antigen in melanoma [PRAME], melanoma antigen family A, 3 [MAGE-A3], tumor protein p53 [p53], and premelanosome protein [gp100]) as well as memory viral responses (influenza A) and chronic viral responses (cytomegalovirus [CMV], Epstein-Barr virus [EBV]).. II. To assess the effect of the vaccine regimen on the ...
The objectives of this study were to determine the safety and efficacy of polyinosinic-polycytidylic acid stabilized with poly-l-lysine and carboxymethylcellulose (poly-ICLC) when added to radiation and temozolomide (TMZ) in adults with newly diagnosed glioblastoma (GB). Patients received external b …
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Phagocytosis of axonal debris is shown by type 1 and 2 microglia. (A) A model of rat cortical explant culture is shown. After the chopped cortex (300-600 μm) was placed in a poly-l-lysine-coated dish, axons grew radially within 7 to 10 days. Explant was subjected to immunofluorescence analysis using an anti-Tuj1 antibody (green) and an anti-Iba1 antibody (red). Scale bar: 500 μm. (B) Coculture of microglia and axonal debris is shown. After primary microglia (4 × 105 cells/dish) were added to the explant culture dish, axons were transected with a scalpel blade (white dotted line). Scale bar: 200 μm. (C) Ninety-six hours after transection of the axons, immunostaining analysis was performed using anti-Tuj1 (green) and anti-Iba1 (red) antibodies. Nuclei were stained with DAPI (blue). Scale bar: 200 μm. (D) High-magnification view of the boxed region in C. Phagocytosed axonal debris was found within the microglia (arrows). Scale bar: 100 μm. (E, F) Type 1 or type 2 microglia (MG) were incubated ...
Cell culture and toxicity studies. Human embryonic kidney cells (HEK293) were cultivated in DMEM supplemented with 10% FCS and 2 mM L-glutamine at 37°C and 5% CO2. Primary neuron cultures were prepared from hippocampi or cortex of C57BL/6 mice and cultured in neurobasal medium supplemented with B-27, N-2, and L-glutamine. All reagents were supplied by Life Technologies (Thermo Fisher Scientific). Neurons were cultivated at different densities depending on which culture plate was used (30,000/well for 96-well plates, 75,000/well for 24-well plates, and 900,000/well for 6-well plates). For confocal laser-scanning microscopy, neurons and HEK293 cells were cultivated on poly-L-lysine-coated and collagenized coverslips, respectively. Unless otherwise stated, all neuronal studies were conducted after 9 to 10 days in vitro (DIV).. Toxicity studies using the MTT assay were conducted in 96-well plates for 12 hours in the presence of 200 μM of each investigated metabolite, unless otherwise stated. For ...
Whether poly-ICLC vaccination is safe and efficacious for patients with melanoma, head and neck cancer, sarcoma, or a non-melanoma skin cancer.
This trial will investigate the efficacy and tolerability of FLT-3 ligand and poly-ICLC in patients with low grade B-cell lymphomas.
This trial will investigate whether delivery of HIV antigens via immunization with anti-DEC-205 p24 monoclonal antibody plus poly ICLC, as an adjuvant, is safe and induces either cellular or humoral immunogenicity in healthy volunteers. We propose to assess the quality of immunity elicited by DEC targeted vaccines in humans. Immunogenicity after HIV antigen delivery directly to dendritic cells could provide the proof-of-concept that dendritic cell targeted protein vaccines may serve as a stand-alone vaccine strategy or in combination with other vaccine modalities against HIV or other diseases.. The main hypothesis of this study is to assess the delivery of HIV antigens via immunization with anti-DEC-205 p24 monoclonal antibody (DCVax-001) plus poly ICLC (Hiltonol) is safe and induces either cellular or humoral immunogenicity in HIV-uninfected, healthy volunteers. ...
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Previous diffusion studies indicated that either endothelial cell- or copper-oxidized LDL binds to a greater extent to type I collagen gel than native LDL, using a gel diffusion technique.9 10 11 In the present study we estimated the binding of Eu3+-LDL and 125I-LDL to microtitration plates coated with the tissue proteins (type I, II, III, IV, and V collagen, fibronectin, and laminin) and poly-d-lysine. This method allowed working with small LDL concentrations and short incubation times, keeping lipid peroxidation low, whereas in studies using diffusion techniques, the incubation time was 48 hours.9 11 Iodination of LDL obviously initiated lipid peroxidation, leading to a considerably higher content of lipid hydroperoxides and resulting in a higher negative charge of LDL protein. A higher negative charge was sometimes obtained with LDL labeled with the Eu3+ chelate. This reagent forms a stable covalent thiourea bond with free amino groups of the LDL protein and therefore causes an ...
Growth cones motility under time modulated tension. (a) Two contacting growth cones of a differentiating rat hippocampal neuron (7 DIV) with an attached poly-D-lysine coated bead. The white arrow indicates the constant force applied on the trapped bead. (b) Traces of the x, y, z coordinates of the tracked bead (in blue, red and green, respectively). Axes origin is at the left upper corner of the field of view in a. Traces are sampled at 2 KHz (kx,y = 4 fN/nm, kz = 2 fN/nm). The blue shadow indicates when the applied force in the x direction is time modulated. Power at the sample, 3.8 mW. Bars, 8 μm. (c) Inset of the traces showed in b. The black line represent the tracked reference point in the x direction indicated in pN on the left side of the plot (oscillating frequency 0.3 Hz ...
Yes. Whilst we have focussed on J774A.1 adherent cells in developing the phagocytosis application, the use of non-adherent phagocytic cell types should be possible. Care must be taken to ensure cells remain in the field of view - this may be facilitated by the use of plate coatings such as poly-D-lysine or Matrigel™.. ...
Animals. Male (225-250 gm) and timed-pregnant female rats (Fischer 344) were purchased from Charles River Laboratories (Raleigh, NC) and kept on a 12 hr light/dark cycle with ad libitum access to food and water. Male rats were acclimated to their environment for 10 d before use for experiments.. Materials. Minimal essential medium (MEM), penicillin, streptomycin, gentamicin, horse serum, and LPS (strain O111:B4) were purchased from Life Technologies (Gaithersburg, MD). [7,8-3H]dopamine (DA) (40 Ci/mmol) was obtained from Amersham Pharmacia Biotech (Arlington Heights, IL) and poly-d-lysine, biotinylated isolectin B4, and dopamine were obtained from Sigma (St. Louis, MO). Biotinylated secondary antibodies, ABC kit, and 3,3′-diaminobenzidine were from Vector Laboratories (Burlingame, CA). The polyclonal anti-tyrosine hydroxylase (TH) antiserum was a gift from Glaxo Wellcome (Research Triangle Park, NC).. LPS injection in vivo. Male rats were anesthetized with sodium pentobarbital (50 mg/kg) and ...
An electrically dimmable rear-view mirror for motor vehicles of simple and robust construction, suitable for use as an outside mirror or as an interior mirror possessing a transmission layer which is composed of a PLZT ceramic, on which a linear birefringence can be imposed by means of a controlling electric field. The transmission layer is located between a linear polarizer and a reflective layer, the latter being designed as a dielectric multilayer arrangement. Suitable comb-type electrodes are located on that side of the reflective layer which faces away from the transmission layer and, if appropriate, on that side of the polarizer which faces the transmission layer, the function of these comb-type electrodes being to generate the controlling field. The polarizer defines the direction of polarization, which is inclined at 45 with respect to the field direction and/or the optical axis of the transmission layer. The strength of the electric field controlling the dimming effect is regulated in a manner
This trial is investigating the tolerability and immunogenicity of poly ICLC as an adjuvant for NY-ESO-1 protein vaccination [cancer vaccine NY-ESO-1], with or