Phosphates, Sugars and a base is the structure of a nucleotide, these are joined together by strong bonds to form the structure of DNA GCSE revision notes
DOLICHOL MONOPHOSPHATE MANNOSE | C106H175O9P | CID 6434507 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
TY - JOUR. T1 - Suppression of Rft1 expression does not impair the transbilayer movement of Man5GlcNAc2-P-P-dolichol in sealed microsomes from yeast. AU - Rush, Jeffrey S.. AU - Gao, Ningguo. AU - Lehrman, Mark A.. AU - Matveev, Sergey. AU - Waechter, Charles J.. PY - 2009/7/24. Y1 - 2009/7/24. N2 - To further evaluate the role of Rft1 in the transbilayer movement of Man5GlcNAc2-P-P-dolichol (M5-DLO), a series of experiments was conducted with intact cells and sealed microsomal vesicles. First, an unexpectedly large accumulation (37-fold) of M5-DLO was observed in Rft1-depleted cells (YG1137) relative to Glc3Man9GlcNAc2-P-P-Dol in wild type (SS328) cells when glycolipid levels were compared by fluorophore-assisted carbohydrate electrophoresis analysis. When sealed microsomes from wild type cells and cells depleted of Rft1 were incubated with GDP-[3H]mannose or UDP-[3H]GlcNAc in the presence of unlabeled GDP-Man, no difference was observed in the rate of synthesis of [3H]Man9GlcNAc2-P-P-dolichol ...
Asn-linked glycosylation of proteins is a universal cotranslational and posttranslational modification of proteins entering the secretory pathway. In all eukaryotes, a hallmark of N-glycosylation is the en bloc transfer of a common preassembled oligosaccharide (Glc3Man9GlcNAc2) from the lipid carrier dolichol pyrophosphate to selected Asn residues in the sequence Asn-X-Ser/Thr (where X ≠ P) within nascent polypeptides. This transfer takes place in the lumen of the endoplasmic reticulum (ER) and is catalyzed by the oligosaccharyltransferase (OST), a heteromeric membrane protein complex (Kelleher and Gilmore, 2006). In yeast (Saccharomyces cerevisiae) and mammals, OST consists of a catalytically active subunit (STAUROSPORIN AND TEMPERATURE SENSITIVE3 [STT3]) and several different noncatalytic subunits that contribute to N-glycosylation by regulation of the substrate specificity, stability, or assembly of the complex (Yan and Lennarz, 2002; Mohorko et al., 2011). In yeast, the OST complex is ...
Molecular Plant-Microbe Interactions 24:1522-1529...Patrycja Zembek,1,2 Urszula Perlińska-Lenart,1 Kurt Brunner,2 Barbara Reithner,2 Grażyna Palamarczyk,1 Robert L. Mach,2 and Joanna S. Kruszewska1...
2) dolichyl β-D-mannosyl phosphate + L-seryl-[protein] = dolichyl phosphate + 3-O-(α-D-mannosyl)-L-seryl-[protein]. For diagram of reaction click here.. Other name(s): dolichol phosphomannose-protein mannosyltransferase; protein O-D-mannosyltransferase; dolichyl-phosphate-D-mannose:protein O-D-mannosyltransferase; dolichyl-phosphate-mannose-protein mannosyltransferase. Systematic name: dolichyl-D-mannosyl-phosphate:protein O-D-mannosyltransferase. Comments: The enzyme transfers mannosyl residues to the hydroxy group of serine or threonine residues, producing cell-wall mannoproteins. It acts only on long-chain α-dihydropolyprenyl derivatives, larger than C35.. Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 74315-99-4. References:. 1. Babczinski, P., Haselbeck, A. and Tanner, W. Yeast mannosyl transferases requiring dolichyl phosphate and dolichyl phosphate mannose as substrate. Partial purification and characterization of the solubilized enzyme. Eur. J. Biochem. ...
Babczinski, P., Haselbeck, A. and Tanner, W. (1980). „Yeast mannosyl transferases requiring dolichyl phosphate and dolichyl phosphate mannose as substrate. Partial purification and characterization of the solubilized enzyme. Eur. J. Biochem. 105: 509-515. PMID 6989607 ...
The chemical reactions and pathways resulting in the formation of dolichol-linked oligosaccharide, usually by a stepwise addition of glycosyl chains to endoplasmic reticulum membrane-bound dolichol-P.
Creative-Proteomics offer cas 50468-56-9 4-O-β-D-galactopyranosyl-D-[1-13C]mannose. We are specialized in manufacturing Stabel Isotope Labeled Analytical Standard products.
DNA a. Structure: Nucleotide Hydrogen Bond -Phosphate -Sugar -Nitrogen Base b. Replication: DNA copies itself exactly and occurs within the Nucleus. A...
Godelaine, D. ; Beaufay, Henri. The Dolichol Pathway of Protein Glycosylation in Rat-liver - Evidence That Gtp Promotes Transformation of Endogenous Dolichyl Phosphate Into Dolichylpyrophosphoryl-n-acetylglucosamine in Stripped Rough Microsomes. In: European Journal of Biochemistry, Vol. 131, no. 3, p. 667-670 (1983 ...
We reported that the C‐terminal 24 amino acids of DPM1 are necessary for association with DPM2 and that Phe21 and Tyr23 in DPM2 are important for association with DPM1 (Maeda et al., 1998). Because the present results suggest that DPM2 associates with DPM1 indirectly through DPM3, these previous observations should be interpreted as indicating that the C‐terminal 24 amino acids of DPM1 are necessary for association with DPM3 and that DPM2 associates with DPM3 using its first transmembrane domain, which includes Phe21 and Tyr23.. DPM1 stabilized with DPM3 in the absence of DPM2 had DPM synthase activity, indicating that DPM2 is not essential for the enzymatic reaction. However, the specific enzymatic activity was 10 times higher when DPM2 was present (Figure 7). Therefore, DPM2 plays a role in the enzymatic reaction. Since DPM2 is very hydrophobic, it seems possible that DPM2 facilitates usage of dolichol‐phosphate. Alternatively, association of DPM2 might cause a steric change in DPM3 that ...
Fingerprint Dive into the research topics of Retinyl Palmitate, Retinyl Phosphate, and Dolichyl Phosphate of Postnuclear Membrane Fraction from Hepatoma, Host Liver, and Regenerating Liver: Marginal Vitamin A Status of Hepatoma Tissue. Together they form a unique fingerprint. ...
The effects of addition of 1 microM-dexamethasone on the rate of transfer of mannose from GDP-mannose into mannolipid have been studied in HeLa cell cultures. Concurrent with an increase in incorporation of mannose into glycoproteins, the incorporation of mannose from GDP-mannose in vitro into mannolipid and dolichol-linked oligosaccharides was increased after dexamethasone treatment. Stimulation of mannolipid synthesis showed a correlation with the 11 beta, 17 alpha, 21-trihydroxy structure of C21 steroids. Dexamethasone treatment also resulted in an increased incorporation of acetate into dolichol and dolichyl phosphate. The results suggest that the effect of dexamethasone on the cell-surface glycoprotein accumulation is related to increased sugar-linked dolichol synthesis. ...
Asparagine-linked glycosylation is one of the most common protein modification reactions in eukaryotic cells, occurring on N-(x≠P)-T/S/C consensus sequons on newly synthesized proteins in the lumen of the rough endoplasmic reticulum (RER). Transfer of the preassembled oligosaccharide (GlcNAc2Man9Glc3) from a dolichol pyrophosphate carrier to sequons is mediated by the oligosaccharyltransferase (OST). Although N-glycosylation is often described as a post-translational protein modification reaction, most N-linked glycans are added to ribosome-bound nascent polypeptides as the protein is passing through the protein translocation channel into the lumen of the RER. A cotranslational mode of N-linked glycosylation ensures that addition of glycan occurs before protein folding, thereby relieving the restriction that sequons reside on surface-exposed loops or within disordered protein segments that can access the OST active site (Lizak et al., 2011).. The OST is a hetero-oligomeric integral membrane ...
J. BADDILEY; Lipid Intermediates in the Biosynthesis of Bacterial Cell-Wall Components. Biochem Soc Trans 1 September 1973; 1 (5): 1026-1028. doi: https://doi.org/10.1042/bst0011026a. Download citation file:. ...
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When pig liver microsomal preparations were incubated with GDP-[14C]mannose, 10-40% of the 14C was transferred to mannolipid and 1-3% to mannoprotein. The transfer to mannolipid was readily reversible and GDP was one of the products of the reaction. It was possible to reverse the reaction by adding excess of GDP and to show the incorporation of [14C]GDP into GDP-mannose. When excess of unlabelled GDP-mannose was added to a partially completed incubation there was a rapid transfer back of [14C]mannose from the mannolipid to GDP-mannose. The other product of the reaction, the mannolipid, had the properties of a prenol phosphate mannose. This was illustrated by its lability to dilute acid but stability to dilute alkali, and by its chromatographic properties. Dolichol phosphate stimulated the incorporation of [14C]mannose into both mannolipid and into protein, although the former effect was larger and more consistent than the latter. The incorporation of exogenous [3H]dolichol phosphate into the ...
THE UNAMBIGUOUS CHARACTERIZATION OF DOLICHOL PHOSPHATE MANNOSE AS A PRODUCT OF MANNOSYL TRANSFERASE IN PIG LIVER ENDOPLASMIC RETICULUM = LA CARACTERISATION NON EQUIVOQUE DU DOLICHOL PHOSPHATE MANNOSE COMME PRODUIT DE LA MANNOSYL TRANSFERASE DANS LE RETICULUM ENDOPLASMIQUE DU FOIE DE PORCEVANS PJ; HEMMING FW.1973; F.E.B.S. LETTERS; NETHERL.; DA. 1973; VOL. 31; NO 3; PP. 335-338; BIBL. 26 REF.Serial Issue ...
N-linked glycosylation of proteins in eukaryotic cells follows a highly conserved pathway. The tetradecasaccharide substrate (Glc3Man9GlcNAc2) is first assembled at the membrane of the endoplasmic reticulum (ER) as a dolichylpyrophosphate (Dol-PP)-linked intermediate, and then transferred to nascent …
DOLPP1 overexpression lysate, 0.1 mg. Transient overexpression lysate of dolichyl pyrophosphate phosphatase 1 (DOLPP1), transcript variant 1
dolichyl beta-D-glucosyl phosphate + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06262 ...
Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).
Looking for online definition of dolichyl phosphate glucosyltransferase in the Medical Dictionary? dolichyl phosphate glucosyltransferase explanation free. What is dolichyl phosphate glucosyltransferase? Meaning of dolichyl phosphate glucosyltransferase medical term. What does dolichyl phosphate glucosyltransferase mean?
K03857 PIGA; phosphatidylinositol N-acetylglucosaminyltransferase subunit A [EC:2.4.1.198] K03859 PIGC; phosphatidylinositol N-acetylglucosaminyltransferase subunit C K03858 PIGH; phosphatidylinositol N-acetylglucosaminyltransferase subunit H K03858 PIGH; phosphatidylinositol N-acetylglucosaminyltransferase subunit H K03861 PIGP; phosphatidylinositol N-acetylglucosaminyltransferase subunit P K03860 PIGQ; phosphatidylinositol N-acetylglucosaminyltransferase subunit Q K11001 PIGY; phosphatidylinositol N-acetylglucosaminyltransferase subunit Y K11001 PIGY; phosphatidylinositol N-acetylglucosaminyltransferase subunit Y K09658 DPM2; dolichol phosphate-mannose biosynthesis regulatory protein K09658 DPM2; dolichol phosphate-mannose biosynthesis regulatory protein K03434 PIGL; N-acetylglucosaminylphosphatidylinositol deacetylase [EC:3.5.1.89] K05283 PIGW; glucosaminylphosphatidylinositol acyltransferase [EC:2.3.-.-] K01127 GPLD1; glycosylphosphatidylinositol phospholipase D [EC:3.1.4.50] K05284 PIGM; ...
The biosynthesis of eukaryotic lipid-linked oligosaccharides (LLOs) that act as donor substrates in eukaryotic protein N-glycosylation starts on the cytoplasmic side of the endoplasmic reticulum and includes
GDP-Man: Man1GlcNAc2-PP-dolichol α-1,3-mannosyltransferase / GDP-Man: Man2GlcNAc2-PP-dolichol α-1,6-mannosyltransferase (Alg2;YGL065c ...
Accepted name: dolichyl-P-Man:Man8GlcNAc2-PP-dolichol α-1,2-mannosyltransferase. Reaction: dolichyl β-D-mannosyl phosphate + D-Man-α-(1→2)-D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→6)]-D-Man-α-(1→6)]-D-Man-β-(1→4)-D-GlcNAc-β-(1→4)-D-GlcNAc-diphosphodolichol = D-Man-α-(1→2)-D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→2)-D-Man-α-(1→6)]-D-Man-α-(1→6)]-D-Man-β-(1→4)-D-GlcNAc-β-(1→4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. For diagram of reaction click here.. Other name(s): ALG9; ALG9 α1,2 mannosyltransferase; dolichylphosphomannose-dependent ALG9 mannosyltransferase; ALG9 mannosyltransferase; Dol-P-Man:Man8GlcNAc2-PP-Dol α-1,2-mannosyltransferase. Systematic name: dolichyl β-D-mannosyl phosphate:D-Man-α-(1→2)-D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→6)]-D-Man-α-(1→6)]-D-Man-β-(1→4)-D-GlcNAc-β-(1→4)-D-GlcNAc-diphosphodolichol ...
Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).
This gene encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel. [provided by RefSeq, Jan 2010 ...
Read Characterization and Localization of a Long‐Chain Isoprenyltransferase Activity in Porcine Brain: Proposed Role in the Biosynthesis of Dolichyl Phosphate, Journal of Neurochemistry on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
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