In the absence of detergent, the transfer of mannose from GDP-mannose to rat liver microsomal vesicles was highly stimulated by exogenous retinyl phosphate in incubations containing bovine serum albumin, as measured in a filter binding assay. Under these conditions 65% of mannose 6-phosphatase activity was latent. The transfer process was linear with time up to 5min and with protein concentration up to 1.5mg/0.2ml. It was also temperature-dependent. The microsomal uptake of mannose was highly dependent on retinyl phosphate and was saturable against increasing amounts of retinyl phosphate, a concentration of 15μm giving half-maximal transfer. The uptake system was also saturated by increasing concentrations of GDP-mannose, with an apparent Km of 18μm. Neither exogenous dolichyl phosphate nor non-phosphorylated retinoids were active in this process in the absence of detergent. Phosphatidylethanolamine and synthetic dipalmitoylglycerophosphocholine were also without activity. Several ...
This gene encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel. [provided by RefSeq, Jan 2010 ...
Fingerprint Dive into the research topics of Retinyl Palmitate, Retinyl Phosphate, and Dolichyl Phosphate of Postnuclear Membrane Fraction from Hepatoma, Host Liver, and Regenerating Liver: Marginal Vitamin A Status of Hepatoma Tissue. Together they form a unique fingerprint. ...
Godelaine, D. ; Beaufay, Henri. The Dolichol Pathway of Protein Glycosylation in Rat-liver - Evidence That Gtp Promotes Transformation of Endogenous Dolichyl Phosphate Into Dolichylpyrophosphoryl-n-acetylglucosamine in Stripped Rough Microsomes. In: European Journal of Biochemistry, Vol. 131, no. 3, p. 667-670 (1983 ...
TY - JOUR. T1 - Suppression of Rft1 expression does not impair the transbilayer movement of Man5GlcNAc2-P-P-dolichol in sealed microsomes from yeast. AU - Rush, Jeffrey S.. AU - Gao, Ningguo. AU - Lehrman, Mark A.. AU - Matveev, Sergey. AU - Waechter, Charles J.. PY - 2009/7/24. Y1 - 2009/7/24. N2 - To further evaluate the role of Rft1 in the transbilayer movement of Man5GlcNAc2-P-P-dolichol (M5-DLO), a series of experiments was conducted with intact cells and sealed microsomal vesicles. First, an unexpectedly large accumulation (37-fold) of M5-DLO was observed in Rft1-depleted cells (YG1137) relative to Glc3Man9GlcNAc2-P-P-Dol in wild type (SS328) cells when glycolipid levels were compared by fluorophore-assisted carbohydrate electrophoresis analysis. When sealed microsomes from wild type cells and cells depleted of Rft1 were incubated with GDP-[3H]mannose or UDP-[3H]GlcNAc in the presence of unlabeled GDP-Man, no difference was observed in the rate of synthesis of [3H]Man9GlcNAc2-P-P-dolichol ...
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K03857 PIGA; phosphatidylinositol N-acetylglucosaminyltransferase subunit A [EC:2.4.1.198] K03859 PIGC; phosphatidylinositol N-acetylglucosaminyltransferase subunit C K03858 PIGH; phosphatidylinositol N-acetylglucosaminyltransferase subunit H K03858 PIGH; phosphatidylinositol N-acetylglucosaminyltransferase subunit H K03861 PIGP; phosphatidylinositol N-acetylglucosaminyltransferase subunit P K03860 PIGQ; phosphatidylinositol N-acetylglucosaminyltransferase subunit Q K11001 PIGY; phosphatidylinositol N-acetylglucosaminyltransferase subunit Y K11001 PIGY; phosphatidylinositol N-acetylglucosaminyltransferase subunit Y K09658 DPM2; dolichol phosphate-mannose biosynthesis regulatory protein K09658 DPM2; dolichol phosphate-mannose biosynthesis regulatory protein K03434 PIGL; N-acetylglucosaminylphosphatidylinositol deacetylase [EC:3.5.1.89] K05283 PIGW; glucosaminylphosphatidylinositol acyltransferase [EC:2.3.-.-] K01127 GPLD1; glycosylphosphatidylinositol phospholipase D [EC:3.1.4.50] K05284 PIGM; ...
DOLICHOL MONOPHOSPHATE MANNOSE | C106H175O9P | CID 6434507 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
We reported that the C‐terminal 24 amino acids of DPM1 are necessary for association with DPM2 and that Phe21 and Tyr23 in DPM2 are important for association with DPM1 (Maeda et al., 1998). Because the present results suggest that DPM2 associates with DPM1 indirectly through DPM3, these previous observations should be interpreted as indicating that the C‐terminal 24 amino acids of DPM1 are necessary for association with DPM3 and that DPM2 associates with DPM3 using its first transmembrane domain, which includes Phe21 and Tyr23.. DPM1 stabilized with DPM3 in the absence of DPM2 had DPM synthase activity, indicating that DPM2 is not essential for the enzymatic reaction. However, the specific enzymatic activity was 10 times higher when DPM2 was present (Figure 7). Therefore, DPM2 plays a role in the enzymatic reaction. Since DPM2 is very hydrophobic, it seems possible that DPM2 facilitates usage of dolichol‐phosphate. Alternatively, association of DPM2 might cause a steric change in DPM3 that ...
Babczinski, P., Haselbeck, A. and Tanner, W. (1980). „Yeast mannosyl transferases requiring dolichyl phosphate and dolichyl phosphate mannose as substrate. Partial purification and characterization of the solubilized enzyme. Eur. J. Biochem. 105: 509-515. PMID 6989607 ...
GDP-Man: Man1GlcNAc2-PP-dolichol α-1,3-mannosyltransferase / GDP-Man: Man2GlcNAc2-PP-dolichol α-1,6-mannosyltransferase (Alg2;YGL065c ...
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2) dolichyl β-D-mannosyl phosphate + L-seryl-[protein] = dolichyl phosphate + 3-O-(α-D-mannosyl)-L-seryl-[protein]. For diagram of reaction click here.. Other name(s): dolichol phosphomannose-protein mannosyltransferase; protein O-D-mannosyltransferase; dolichyl-phosphate-D-mannose:protein O-D-mannosyltransferase; dolichyl-phosphate-mannose-protein mannosyltransferase. Systematic name: dolichyl-D-mannosyl-phosphate:protein O-D-mannosyltransferase. Comments: The enzyme transfers mannosyl residues to the hydroxy group of serine or threonine residues, producing cell-wall mannoproteins. It acts only on long-chain α-dihydropolyprenyl derivatives, larger than C35.. Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 74315-99-4. References:. 1. Babczinski, P., Haselbeck, A. and Tanner, W. Yeast mannosyl transferases requiring dolichyl phosphate and dolichyl phosphate mannose as substrate. Partial purification and characterization of the solubilized enzyme. Eur. J. Biochem. ...
THE UNAMBIGUOUS CHARACTERIZATION OF DOLICHOL PHOSPHATE MANNOSE AS A PRODUCT OF MANNOSYL TRANSFERASE IN PIG LIVER ENDOPLASMIC RETICULUM = LA CARACTERISATION NON EQUIVOQUE DU DOLICHOL PHOSPHATE MANNOSE COMME PRODUIT DE LA MANNOSYL TRANSFERASE DANS LE RETICULUM ENDOPLASMIQUE DU FOIE DE PORCEVANS PJ; HEMMING FW.1973; F.E.B.S. LETTERS; NETHERL.; DA. 1973; VOL. 31; NO 3; PP. 335-338; BIBL. 26 REF.Serial Issue ...
Creative-Proteomics offer cas 50468-56-9 4-O-β-D-galactopyranosyl-D-[1-13C]mannose. We are specialized in manufacturing Stabel Isotope Labeled Analytical Standard products.
dolichyl beta-D-glucosyl phosphate + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06262 ...
Looking for online definition of dolichyl phosphate glucosyltransferase in the Medical Dictionary? dolichyl phosphate glucosyltransferase explanation free. What is dolichyl phosphate glucosyltransferase? Meaning of dolichyl phosphate glucosyltransferase medical term. What does dolichyl phosphate glucosyltransferase mean?
Molecular Plant-Microbe Interactions 24:1522-1529...Patrycja Zembek,1,2 Urszula Perlińska-Lenart,1 Kurt Brunner,2 Barbara Reithner,2 Grażyna Palamarczyk,1 Robert L. Mach,2 and Joanna S. Kruszewska1...
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Accepted name: dolichyl-P-Man:Man8GlcNAc2-PP-dolichol α-1,2-mannosyltransferase. Reaction: dolichyl β-D-mannosyl phosphate + D-Man-α-(1→2)-D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→6)]-D-Man-α-(1→6)]-D-Man-β-(1→4)-D-GlcNAc-β-(1→4)-D-GlcNAc-diphosphodolichol = D-Man-α-(1→2)-D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→2)-D-Man-α-(1→6)]-D-Man-α-(1→6)]-D-Man-β-(1→4)-D-GlcNAc-β-(1→4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. For diagram of reaction click here.. Other name(s): ALG9; ALG9 α1,2 mannosyltransferase; dolichylphosphomannose-dependent ALG9 mannosyltransferase; ALG9 mannosyltransferase; Dol-P-Man:Man8GlcNAc2-PP-Dol α-1,2-mannosyltransferase. Systematic name: dolichyl β-D-mannosyl phosphate:D-Man-α-(1→2)-D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→2)-D-Man-α-(1→3)-[D-Man-α-(1→6)]-D-Man-α-(1→6)]-D-Man-β-(1→4)-D-GlcNAc-β-(1→4)-D-GlcNAc-diphosphodolichol ...
N-linked glycosylation of proteins in eukaryotic cells follows a highly conserved pathway. The tetradecasaccharide substrate (Glc3Man9GlcNAc2) is first assembled at the membrane of the endoplasmic reticulum (ER) as a dolichylpyrophosphate (Dol-PP)-linked intermediate, and then transferred to nascent …