Ralstonia eutropha H16 is a well-studied bacterium with respect to biosynthesis of polyhydroxyalkanoates (PHAs), which has attracted attentions as biodegradable bio-based plastics. However, this strain shows quite poor growth on glycerol of which bulk supply has been increasing as a major by-product of biodiesel industries. This study examined enhancement of glycerol assimilation ability of R. eutropha H16 by introduction of the genes of aquaglyceroporin (glpF) and glycerol kinase (glpK) from Escherichia coli. Although introduction of glpFK Ec into the strain H16 using a multi-copy vector was not successful, a recombinant strain possessing glpFK Ec within the chromosome showed much faster growth on glycerol than H16. Further analyses clarified that weak expression of glpK Ec alone allowed to establish efficient glycerol assimilation pathway, indicating that the poor growth of H16 on glycerol was caused by insufficient kination activity to glycerol, as well as this strain had a potential ability ...

The synthesis and degradation of polyhydroxyalkanoates (PHAs), the storage polymer of many bacteria, is linked to the operation of central carbon metabolism. To rationalize the impact of PHA accumulation on central carbon metabolism of the prototype bacterium Pseudomonas putida, we have revisited PHA production in quantitative physiology experiments in the wild-type strain vs. a PHA negative mutant growing under low nitrogen conditions. When octanoic acid was used as PHA precursor and as carbon and energy source, we have detected higher intracellular flux via acetyl-CoA in the mutant strain than in the wild type, which correlates with the stimulation of the TCA cycle and glyoxylate shunt observed on the transcriptional level. The mutant defective in carbon and energy storage spills the additional resources, releasing CO2 instead of generating biomass. Hence, P. putida operates the metabolic network to optimally exploit available resources and channels excess carbon and energy to storage via PHA, ...

TY - JOUR. T1 - Isolation of polyhydroxyalkanoate from hydrolyzed cells of Bacillus flexus using aqueous two-phase system containing polyethylene glycol and phosphate. AU - Divyashree, M. S.. AU - Shamala, T. R.. AU - Rastogi, N. K.. PY - 2009/10/9. Y1 - 2009/10/9. N2 - Main objective of present work was to isolate polyhydroxyalkanoate (PHA) from cell lysate of Bacillus flexus by aqueous-aqueous two-phase system (ATPS). Selected ATPS having polyethylene glycol (12%, w/v) and potassium phosphate (9.7%, pH 8.0) containing cell lysate obtained by sonication or hypochlorite treatment of B. flexus biomass (1 g%, dry weight), was held at 28°C for 30 min, which partitioned PHA into top PEG phase and residual cell materials into bottom phase. For enzymatic cell hydrolysis, Microbispora sp. culture filtrate having protease (3 U/mL) was mixed with B. flexus biomass and ATPS, incubated at 37°C for 2 h prior to phase separation. PHA recovered by centrifugation was 19∼51% of cell dry weight, depending on ...

de Eugenio, L.I., García, P., Luengo, J.M., Sanz, J., San Román, J., García, J.L., and Prieto, M.A. (2007) Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: characterization of a paradigmatic enzyme. J Biol Chem 282: 4951-4962 ...

Achieving a sustainable society requires, among other things, the use of renewable feedstocks to replace chemicals obtained from petroleum-derived compounds. Crude glycerol synthesized inexpensively as a byproduct of biodiesel production is currently considered a waste product, which can potentially be converted into value-added compounds by bacterial fermentation. This study aimed at evaluating several characterized P. putida strains to produce medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA) using raw glycerol as the only carbon/energy source. Among all tested strains, P. putida KT2440 most efficiently synthesized mcl-PHA under nitrogen-limiting conditions, amassing more than 34% of its cell dry weight as PHA. Disruption of the PHA depolymerase gene (phaZ) in P. putida KT2440 enhanced the biopolymer titer up to 47% PHA (%wt/wt). The low biomass and PHA titer found in the mutant strain and the wild-type strain KT2440 seems to be triggered by the high production of the side-product citrate during

Achieving a sustainable society requires, among other things, the use of renewable feedstocks to replace chemicals obtained from petroleum-derived compounds. Crude glycerol synthesized inexpensively as a byproduct of biodiesel production is currently considered a waste product, which can potentially be converted into value-added compounds by bacterial fermentation. This study aimed at evaluating several characterized P. putida strains to produce medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA) using raw glycerol as the only carbon/energy source. Among all tested strains, P. putida KT2440 most efficiently synthesized mcl-PHA under nitrogen-limiting conditions, amassing more than 34% of its cell dry weight as PHA. Disruption of the PHA depolymerase gene (phaZ) in P. putida KT2440 enhanced the biopolymer titer up to 47% PHA (%wt/wt). The low biomass and PHA titer found in the mutant strain and the wild-type strain KT2440 seems to be triggered by the high production of the side-product citrate during

Sabian HHX Stage Crash CymbalMedium weight of the Sabian 18 HHX Stage Crash boosts volume for solid attack and full-bodied, warm, penetrating response. This best-selling Core Sabian model is a Killer Crash! Until HHX, it was impossible to project darkness. But the dark, simmering sound, agitated bite and Tone Projection design of HHX makes this series more effective than others. Other cymbals may generate darkness, but HHX stirs up more simmering heat, more musical dirt, more of what drummers want to hear. Manufacturer: SabianModel: 11808XNWarranty: See manufacturer website for detailsSKU: I138909-GA1-1-BUPC: 622537029790We are Chuck Levins Washington Music CenterMaryland, Virginia, and Washington DCs legendary music shop. Family-owned and operated since 1958. Buy gear the way it was meant to be bought, from a store thats been there through it all and has the vibe to prove it. Were staffed with musicians and industry experts, people that live and breathe music gear. Our

1.Chen GQ, Wu Q. 2005. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials. 26:6565-6578 2. Doi Y, Kunioka M, Nakamura Y, Soga K. 1986. Nuclear magnetic resonance studies on poly(B-hydroxybutyrate) and a copolyester of B-hydroxybutyrate and B-hydroxyvalerate isolated from Alcaligenes eutrophus H16. Macromolecules. 19:2860-2864 3. Endy D. 2005. Foundations for engineering biology. Nature. 438(7067):449-53 4. International Genetically Engineered Machines competition. 15 Jun 2008. 26 Jul 2008. ,http://igem.org, 5. Holmes PA. 1985. Applications of PHB - a microbially produced biodegradable thermoplastic. Physics in technology. 16:32-36 6. Kang Z, Wang Q, and H Zhang. 2008. Construction of a stress-induced system in Escherichia coli for efficient polyhydroxyalkanoates production. Biotechnological Products and Process Engineering. 79:203-208 7. Knight TF. 2003. Idempotent Vector Design for Standard Assembly of BioBricks. Tech. rep., MIT Synthetic Biology Working ...

AB - The polyhydroxyalkanoates (PHAs) are microbially-produced biopolymers that could potentially be used as sustainable alternatives to oil-derived plastics. However, PHAs are currently more expensive to produce than oil-derived plastics. Therefore, more efficient production processes would be desirable. Cell-free metabolic engineering strategies have already been used to optimise several biosynthetic pathways and we envisioned that cell-free strategies could be used for optimising PHAs biosynthetic pathways. To this end, we developed several Escherichia coli cell-free systems for in vitro prototyping PHAs biosynthetic operons, and also for screening relevant metabolite recycling enzymes. Furthermore, we customised our cell-free reactions through the addition of whey permeate, an industrial waste that has been previously used to optimise in vivo PHAs production. We found that the inclusion of an optimal concentration of whey permeate enhanced relative cell-free GFPmut3b production by ∼50%. In ...

A new bacterium, designated as strain TE9 was isolated from a microbial mat in French Polynesia and was studied for its ability to synthesize medium chain length poly-beta-hydroxyalkanoates (mcl PHAs) during cultivation on cosmetics co-products. The composition of PHAs was analysed by coupled gas chromatography mass spectroscopy (GC/MS), nuclear magnetic resonance (NMR) and Fourier Transform InfraRed (FTIR) spectroscopy. PHAs were composed of C6-C14 3-hydroxyacids monomers, with a predominance of 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD) and 3-hydroxydodecanoate (3HDD). Differential scanning calorimetry (DSC) experiments allowed the characterization of elastomeric materials with a melting point T(m) near 50 degrees C, enthalpy of fusion DeltaH(m) from 27 to 32 J/g, and glass transition temperature T(g) of -43 degrees C. Molecular weights ranged from 175,000 to 358,000 g/mol. On the basis of the phenotypical features and genotypic investigations, strain TE9 was assigned to the Pseudomonas genus

Domain architectures containing the following SCOP superfamilies _gap_,100950,_gap_ in Pseudomonas mendocina NK-01. Domain architectures illustrate each occurrence of _gap_,100950,_gap_.

In California, researchers at the University of California, Berkeley have coaxed Escherichia coli to use fluorinated building blocks to make new organofluorine target molecules.. Led by with Michelle C.Y. Chan, the group able to introduce genes to code for enzymes that use fluorine-containing derivatives of their normal substrates. The researchers also introduced a gene for an enzyme used by many bacteria to make polyhydroxyalkanoates (PHAs), a biodegradable bioplastic. The fluorinated versions of PHA were less brittle than conventional PHAs, opening up new applications. The researchers also hope to produce small fluorinated molecules in living cells for pharmaceuticals. ...

Caracterización de la fasina PhaF de Pseudomonas putida KT2442 y de su unión a polímeros de poli-3-hidroxibutirato Conference Paper 2008 ...

Abstract: Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is one of the components of polyhydroxyalkanoates (PHAs) and some of its mechanical properties have been shown to improve over poly (3-hydroxybutyrate) (PHB) and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The investigation of PHBHHx microspheres as a drug delivery system was prepared by emulsion-solvent evaporation method for the sustained release of anti-cancer drug 5-fluorouracil (5-FU) and cyclosporin A (CsA). The mean diameter of the PHBHHx microspheres ranged from 5.24 to 22.10 μm dependent on the different processing parameters. The PHBHHx concentration, emulsifier concentration, anti-cancer drug dosage, and agitation speed, were optimized according to the encapsulation efficiency of 4% PHBHHx, 0.5% SDS, 10 mg anti-cancer drug, and 500 rpm. Under optimized conditions, the encapsulation efficiency of 5-FU and CsA microspheres were 7.19% and 96.44%, respectively. The morphologies of scanning electron microscope ...

23. The norbornene polymer according to claim 8, wherein the norbornene polymer is a copolymer further comprising a polar norbornene repeating unit represented by Formula 3 and a nonpolar norbornene repeating unit represented by Formula 4:wherein each m is independently an integer of 0 to 4,at least one of R1a, R2a, R3a, and R4a is a polar functional group containing at least one atom selected from the group consisting of oxygen, nitrogen, phosphorus, sulfur, silicon, and boron, andR1a, R2a, R3a, and R4a that are not the polar functional group are each independently hydrogen; halogen; linear or branched alkyl, alkenyl, or vinyl having 1 to 20 carbon atoms; cycloalkyl that is unsubstituted or substituted with hydrocarbon and has 5 to 12 carbon atoms; aryl that is unsubstituted or substituted with hydrocarbon and has 6 to 40 carbon atoms; aralkyl that is unsubstituted or substituted with hydrocarbon and has 7 to 15 carbon atoms; alkynyl that has 2 to 20 carbon atoms; linear or branched haloalkyl, ...

The bio-transformation of organic crops, such as corn, has led to a range of polyesters such as poly lactic acid (PLA) and polyhydroxyalkanoates (PHA). ). Applications of these polyesters could include paper coatings, wood product finishing, adhesives, and waterborne coating technologies. Researchers at UNH are preparing waterborne hybrid vinylic polyester latices through a miniemulsion polymerization process ...

Some of the top companies like Asta Real and Fuji Health have doubled its production capacity recently owing to the increasing demand of astaxanthin.. Lutein, a carotenoid extracted from algae, is capable of preventing the onset of Age-related Macular Degeneration (AMD). The global market value of lutein was around $233 million in 2010 and is expected to reach $309 million by 2018.. Algae are also widely used in the production of enzymes and toxins which, in general, have a product values in the range of US $4000-8000/kg. Algae are also used in cosmetics industry as thickening agents, water-binding agents and antioxidants.. Algae based added value commodities such as lactic acid, polyhydroxyalkanoates and butanol price ranges from US $1300 to US $7000/tonne.. Learn more on the business opportunities in algae non-fuel products from Comprehensive Report on Attractive Algae Product Opportunities. ...

The results of the stereolithographic habitat construction process of Cesaretti et al. [11] can also be accomplished with a three-dimensional printer that extrudes plastic media using fused deposition modelling (FDM). A comparison of the different additive manufacturing methods and possible feedstocks is included in [49], which explains why FDM is preferred over stereolithography for space applications. To determine the existence of launch mass savings for three-dimensional printed habitats from biological processes, this section examines the extraterrestrial manufacture of feasible three-dimensional printer raw materials like polyhydroxyalkanoate (PHA) biopolymers, of which polyhydroxybutyrates (PHBs) are an example. A contrast of habitat structural properties when using either salt-MgO or biopolymers as printer media is beyond the scope of this paper.. The biomanufacture of PHAs is reviewed in [122-125], and Lu et al. [123] include a list of organisms known to synthesize PHAs. It has been ...

Its time to talk about something that almost everyone can use without being worried about breaking out. AHAs and BHAs are fantastic chemical exfoliants but weve heard way too many cases of people breaking out or purging periods being too long. Fear no more ladies for its time to welcome PHAs into our lives. So, what

A process for producing a toner for developing electrostatic images is disclosed. A polymerizable monomer composition is first prepared and dispersed in an aqueous medium by the action of high shear force to produce particles. The polymerizable monomer is then polymerized in a container having a jacket. The liquid temperature inside the container is controlled by feeding a high-pressure vapor into the jacket while adjusting the degree of vacuum inside the jacket. The production process can achieve a superior temperature response, can keep the reaction system at a constant temperature with ease and can realize shortened production time and reduced production cost.

Supplementary MaterialsSupplementary Info 41598_2019_39321_MOESM1_ESM. of promoter engineering for raising the produce of mcl-PHA in NK-01. In this ongoing work, 10 endogenous promoters from NK-01 had been identified predicated on promoter and RNA-seq prediction outcomes. Subsequently, 10 putative promoters had been characterized for his or her power through the manifestation of the reporter gene operon in the genome of NK-01, leading to the recombinant strains NKU-4C1, NKU-16C1 and NKU-6C1. Needlessly to say, the transcriptional degrees of and in the recombinant strains had been increased as demonstrated by real-time quantitative RT-PCR. The gene encoding PHA depolymerase was erased to create the recombinant strains NKU- further?NK-01 and highlights the potential of the screened endogenous solid promoters for metabolic executive of NK-01 to improve the produce of mcl-PHA. Intro Currently, promoter executive can serve as a robust tool for logical tuning of the experience of the artificial ...

Metabolix biopolymers are based on polyhydroxyalkanoate polymers (PHAs) and are made by fermentation.. using renewable carbon based feedstocks, making them 100% biobased in neat form.. ...

Large-scale production and purification of recombinant proteins by cell cultures represent a key-area in manufacturing field. The production process still has several drawbacks affecting cost/efficiency. In this work, three modular systems were designed to overcome some of these bottlenecks. A library of self-inducible promoters was built and characterized to start the peptide production at a desired culture density, without expensive inducer molecules. Two standard integrative vectors were realized to insert BioBrick parts in user-defined positions of E. coli or S. cerevisiae genome, to ensure genetic stability without using selection markers. Finally, two promising techniques were combined for an in-cell protein purification: PolyHydroxyAlkanoate (PHA) granules were used as a substrate for PHA-binding peptides (Phasins) fused to the target protein, thus replacing affinity resins/columns and tags, while a pH-based self-cleaving peptide (Intein) was used instead of a protease cleavage site. ...

Large-scale production and purification of recombinant proteins by cell cultures represent a key-area in manufacturing field. The production process still has several drawbacks affecting cost/efficiency. In this work, three modular systems were designed to overcome some of these bottlenecks. A library of self-inducible promoters was built and characterized to start the peptide production at a desired culture density, without expensive inducer molecules. Two standard integrative vectors were realized to insert BioBrick parts in user-defined positions of E. coli or S. cerevisiae genome, to ensure genetic stability without using selection markers. Finally, two promising techniques were combined for an in-cell protein purification: PolyHydroxyAlkanoate (PHA) granules were used as a substrate for PHA-binding peptides (Phasins) fused to the target protein, thus replacing affinity resins/columns and tags, while a pH-based self-cleaving peptide (Intein) was used instead of a protease cleavage site. ...

Record removed. The sequence NP_745242 is 100% identical to WP_003248355.1 over its full length. Be aware that a NCBI nonredundant RefSeq protein (WP_) can be annotated on large numbers of bacterial genomes that encode that identical protein.. Old NP_745242.1 New WP_003248355.1 Identical proteins Re-annotation project. ...

Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2020 Elsevier B.V. We use cookies to help provide and enhance our service and tailor content. By continuing you agree to the use of cookies. Log in to Pure. ...

If he chooses to use only Styrofoam items (which weighs much less than PLA and plastic items) - in one year, he will have consumed 191.11 kWh of energy, released 95.81 pounds of CO2 and used 254.59 gallons of water. Since the average household uses 29.19 kWh of energy per day, this would be enough energy to power a household for 6.55 days ...

Richée Professional Bioplastic Progressive Brush reduces the volume of undisciplined and rebellious hair. Its formula, enriched with highly nutritious ingredients, will leave your hair smooth and with a renewed structure.Professional Bioplastic Richée restores moisture to the hair fiber, essential for the health and mo

Proposed maintenance/housekeeping building; gas storage compound; staff car parking; temporary lodge storage area Ref P/01428/14 TCP/20245/ ...

chains in the Genus database with same CATH superfamily 3C1Y A; 4Z6D A; 1KFD A; 5D49 A; 4I2I A; 3MR2 A; 4YMO A; 3GII A; 2BPF A; 1T7P A; 4PHA A; 4RNN A; 4QWC A; 4CCH A; 1ARO P; 1ZQK A; 5KFB A; 3MFH A; 5HP4 A; 5KT3 A; 1Z00 B; 4YVZ A; 3KHH A; 3THV A; 3RBE A; 3RJK A; 2J6T A; 1SKR A; 1MQ3 A; 4YXM A; 1U47 A; 5J2H A; 4Q45 A; 2H5X A; 4UAZ A; 2XY6 A; 3LDA A; 5KFE A; 4J9L A; 5DB9 A; 3KHR A; 2Y1J A; 2MUT B; 9ICP A; 3C1Z A; 2GWS A; 3MBY A; 3BDP A; 4FBU A; 4DSL A; 1SL2 A; 5CP2 A; 4DQP A; 2DFL A; 2VA8 A; 3BQ0 A; 5CHG A; 4XRH A; 1SL1 A; 3RJG A; 3OHA A; 4JK1 A; 9ICT A; 2I1Q A; 4C8O A; 3RRH A; 2ASD A; 1QSY A; 2W8L A; 2R8H A; 2Y1I A; 3HPO A; 1BPY A; 2PFO A; 2ZUC A; 3V7J A; 2HHX A; 2KZZ A; 1QLN A; 4UB5 A; 1K1Q A; 3V7K A; 1T4G A; 4ZH4 A; 5J2K A; 2R8I A; 3UXP A; 4PPX A; 4MF2 A; 4O3O A; 3ETL A; 4F91 B; 5J0R A; 1TK0 A; 4RNO A; 4RQ0 A; 2MUT A; 4TUR A; 3TFR A; 1ZQA A; 1D9F A; 4P4M A; 3MGI A; 3ZDA A; 4QZA A; 4GC6 A; 3FYH A; 5KFC A; 4ECZ A; 3T3F A; 3C5G A; 2PI5 A; 4YFK A; 9ICK A; 7ICV A; 4UB3 A; 2NRZ A; 3C23 A; 4NLG A; ...

He is clean necked, big hipped and has a killer look when you study him from the profile.. Hair like a carrier, but is THF and PHAF to breed to your carrier cows.. Winning is in his pedigree, as his dam is the full sister to the dam of the 2015 NWSS grand champion steer.. ...

Every month, a womans body prepares itself for the possibility of conception and pregnancy by going through a menstrual cycle. During the menses phas...

Genentech, a member of the Roche Group (SIX: RO, ROG; OTCQX: RHHBY) today announced positive topline results from two identically designed global Phas

Can you name the Phar 401 Druglist Test your knowledge on this science quiz to see how you do and compare your score to others. Quiz by mpchiang

Study Phar1603 using smart web & mobile flashcards created by top students, teachers, and professors. Prep for a quiz or learn for fun!

Visiongain publishes insightful market-led reports that will help you understand future opportunities from changes in technologies, materials & regulations.

Visiongain publishes insightful market-led reports that will help you understand future opportunities from changes in technologies, materials & regulations.

Polyhydroxybutyrate (PHB) is one of the polyhydroxyalkanoates (PHAs) which has biodegradable and biocompatible properties. They are adopted in the biomedical field, in, for example, medical implants and drug delivery carriers. This study seeks to promote the production of PHB by Vibrio sp. BM-1, isolated from a marine environment by improving constituents of medium and implementing an appropriate fermentation strategy. This study successfully developed a glycerol-yeast extract-tryptone (GYT) medium that can facilitate the growth of Vibrio sp. BM-1 and lead to the production of 1.4 g/L PHB at 20 h cultivation. This study also shows that 1.57 g/L PHB concentration and 16% PHB content were achieved, respectively, when Vibrio sp. BM-1 was cultivated with MS-GYT medium (mineral salts-supplemented GYT medium) for 12 h. Both cell dry weight (CDW) and residual CDW remained constant at around 8.2 g/L and 8.0 g/L after the 12 h of cultivation, until the end of the experiment. However, both 16% of PHB content and

A novel method for the assay of polyhydroxyalkanoate (PHA)-degrading ability of triacylglycerol lipases was developed. By applying the natural affinity of lipases towards hydrophobic interfaces, a sensitive and rapid densitometry analysis for the evaluation of hydrolytic activity of lipase droplets towards PHA-coated surface was successfully carried out. We found that 12 out of 14 tested lipases which are of fungal, bacterial and animal origin were able to hydrolyze P(3HB-co-92 mol% 4HB) thin film. The patterns and opacity of the hydrolysis spots of lipases on PHA films allowed easy comparison of PHA-hydrolytic strength of lipases. Lipase from the bacterium Chromobacterium viscosum exhibited the highest PHA-degrading activity. The hydrolytic activity of lipases on water insoluble PHA, emulsified p-nitrophenyl laurate and olive oil were also compared and interestingly some lipases showed better activity when PHA was used as a substrate.

The microbial synthesis of polyhydroxyalkanoates (PHA) from organic wastes is a valuable process to valorize available renewable resources, such as food wastes and biological sludge. Bioplastics find many applications in various sectors, from medical field to food industry. However, persistent organic pollutants could be transferred from wastes to the final product. The present paper demonstrates that the use of municipal wastes in PHA production is safe for the environment and human health and provides a polychlorinated biphenyl (PCB) profile in both commercial and waste-based PHA samples. PCB analysis in several PHA samples showed very low concentrations of the target analytes. Commercial PHA samples showed a similar PCB level with respect to PHA samples from municipal waste/sludge and higher than PHA samples from fruit waste. For all analyzed PCBs, detected concentrations were consistently lower than the ones reported in regulatory framework or guidelines.

phdthesis{de533308-cdd5-4693-8db6-38f644972c19, abstract = {This thesis presents studies on moderately halophilic bacteria belonging to the family Halomonadaceae that accumulate polyhydroxybutyrate (PHB) in high amounts when grown under conditions of nitrogen limitation. PHB is the simplest homopolymer belonging to the class of polyhydroxyalkanoates (PHAs), the biopolyesters that can biodegraded in the environment to carbon dioxide and water and are hence attractive substitutes for fossil-derived plastics. ,br/,,br, Halomonas andesensis was isolated from a hypersaline lake in the Andean region of Bolivia, and was identified as a novel species by phenotypic and genotypic characterization. The organism utilizes different carbon sources such as acetate, L-arabinose, cellobiose, D-fructose, galactose, D-gluconic acid, D-glucose, L-lactic acid, maltose, myo-inositol, propionic acid, raffinose, L-rhamnose, sucrose, starch and D-xylose, respectively, for growth. ,br/,,br, Halomonas boliviensis was also ...

Picture Copyright Dennis Kunkel Microscopy, Inc.. P4SB is about the utilisation of the conceptual and material tools of contemporary Synthetic Biology to bring about the sustainable and environmentally friendly bioconversion of oil-based plastic waste into fully biodegradable counterparts by means of deeply engineered, whole-cell bacterial catalysts. These tools will be used to design tailor-made enzymes for the bio-depolymerisation of PET (polyethylene terephthalate) and PU (polyurethane), but also for the custom design of a Pseudomonas putida Cell Factory capable of metabolizing the resulting monomers. Pseudomonas putida will undergo deep metabolic surgery to channel these diverse substrates efficiently into the production of polyhydroxyalkanoates (PHA) and derivatives. In addition, synthetic downstream processing modules based on the programmed non-lytic secretion of PHA will facilitate the release and recovery of the bioplastic from the bacterial biomass. These industry driven objectives ...

Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, growth kinetics and stoichiometry, to gain a systematic understanding of the underlying metabolic and regulatory network. Glycerol-grown P. putida KT2440 has a maintenance energy requirement [0.039 (mmolglycerol (gCDW h)(-1))] that is about sixteen times lower than that of other bacteria, such as Escherichia coli, which provides a great advantage to use this substrate commercially. The shift from carbon (glycerol) to nitrogen (ammonium) limitation drives the modulation of specific genes involved in glycerol ...

Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, growth kinetics and stoichiometry, to gain a systematic understanding of the underlying metabolic and regulatory network. Glycerol-grown P. putida KT2440 has a maintenance energy requirement [0.039 (mmolglycerol (gCDW h)−1)] that is about sixteen times lower than that of other bacteria, such as Escherichia coli, which provides a great advantage to use this substrate commercially. The shift from carbon (glycerol) to nitrogen (ammonium) limitation drives the modulation of specific genes involved in glycerol

Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, growth kinetics and stoichiometry, to gain a systematic understanding of the underlying metabolic and regulatory network. Glycerol-grown P. putida KT2440 has a maintenance energy requirement [0.039 (mmolglycerol (gCDW h)−1)] that is about sixteen times lower than that of other bacteria, such as Escherichia coli, which provides a great advantage to use this substrate commercially. The shift from carbon (glycerol) to nitrogen (ammonium) limitation drives the modulation of specific genes involved in glycerol

Fingerprint Dive into the research topics of Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers. Together they form a unique fingerprint. ...

Wide use of synthetic plastics increases the environmental concerns of non-biodegradable wastes which home pathogenic organisms and contamination with particles of toxic compounds leached into ground water and other organic pollutants associated with plastics wastes. This research aimed at developing a biodegradable polymer using plantain peels flour with Bacillius siamensis kcTcT 13613(T). During fermentation, optical density, pH and incubation time were monitored. After 48 hours of incubation, the polymer were extracted using chloroform and 63% dried cell weight were gotten. FT-IR analysis peak at wave numbers 1656 cm-1 revealed ester carbonyl (C=O) stretching groups, the GC-MS Chromatogram apex at (7.957) which correspond to polyhydroxybutyrate spectral. This study provides economic insight use of plantain peels cultured with Bacillus Siamensis KcTc 13613(T) is capable of producing biodegradable polymer (PHB) that is environmental friendly.. ...

If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. Thank you!. Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016 ...

Sigma-Aldrich offers abstracts and full-text articles by [J L Castro-Mayorga, A Martínez-Abad, M J Fabra, Catarina Olivera, M Reis, J M Lagarón].

The influence of leukemic serum on phytohemagglutinin-P (PHA-P)-induced lymphocyte transformation was studied. Twenty of 22 pretreatment sera collected at diagnosis inhibited the transformation of normal lymphocytes stimulated with PHA-P by more than 50%. In these experiments with PHA-P, kinetic studies of transformation demonstrated that leukemic serum inhibited rather than delayed transformation. The inhibition of transformation by PHA-P was assumed to be due to binding of PHA by components of leukemic serum because the inhibition could be overcome by an increase in the PHA concentration. A biologic assay of the PHA content of lysates of lymphocytes preincubated with PHA and leukemic serum further substantiated the PHA-binding mechanism of leukemic serum. Whether the differences observed in the binding capacity of normal and leukemic serum are qualitative or quantitative remains to be determined.. ...

View the basic SGB.V stock chart on Yahoo Finance. Change the date range, chart type and compare SOLEGEAR BIOPLASTIC TECH INC against other companies.

Pulsed laser polymerization coupled with size exclusion chromatography (PLP-SEC) was implemented to study the micellar radical homopropagation kinetics of cationic surfmer, polycaprolactone choline iodide ester methacrylate (PCLnChMA with n = 2 average polyester units), at concentrations of 5, 10, and 20 wt% in aqueous solutions at 25, 50, 70, and 85 °C. PCL2ChMA can propagate in both aqueous and compartmentalized phases, with the relative importance of the two reaction loci changing with temperature. As the corresponding saturated macromonomer concentration, [M], inside the growing polymeric micelles cannot be easily determined, only the product of propagation rate coefficient (kp) and [M] are measured by PLP-SEC; at 25 °C, a minimum kp of 863 ± 95 L mol−1 s−1 is estimated assuming bulk [M]. (Macro)monomer composition drifts for batch acrylamide (AM)/PCLnChMA micellar copolymerizations in D2O at 50 °C are well represented by the apparent reactivity ratios rAM = 0.31 ± 0.03 and ...

A complex comprises organoborane and polyamine. The polyamine is the reaction product of a diprimary amine-terminated material and a material having at least two groups reactive with primary amine. The complexes are useful in systems for initiating the polymerization of acrylic monomer, which systems further include a material reactive with amine. Polymerizable acrylic monomer compositions useful as adhesives can be prepared.

The effect of the granule structure on the methylation of starch was investigated by comparing the substitution patterns of potato starch methylated in granular suspension and in solution to DS 0.3. Substitution patterns were analyzed by successive digestion with α-amylase and amyloglucosidase, fractionation of the resulting malto-oligosaccharide mixture by GPC on a preparative scale, and characterization of the fractions by GLC and MALDI-MS. The mass composition of fractions with intermediate and higher degree of polymerization was indicative of enhanced clustering of substituents in granular methyl starch. On the contrary, the composition of the smaller saccharides was governed by enzyme specificity, which was also reflected in strong deviations in their monomer composition. A sequencing study on selected pure small saccharides confirmed and complemented previous conclusions on enzyme specificity. © 2008 Elsevier Ltd. All rights reserved.. ...

A purpose of the present invention is to provide a production method by which a poly(hydroxyalkanoic acid) (PHA) (e.g., a PHA powder) can be obtained at high efficiency. This PHA production method comprises (a) the step of producing an a ...

If you have used this database, please ensure that you acknowledge this most recent Pseudomonas Genome Database publication rather than just the website URL. Thank you!. Winsor GL, Griffiths EJ, Lo R, Dhillon BK, Shay JA, Brinkman FS (2016 ...

Genome Portal version:8.18.66 content:a67cb9e668 jgi-portal-web-2 Release Date:07-Jun-2021 16:04:17.646 PST Current Date:19-Jun-2021 06:15:42.695 PDT ...

However, in order to be considered for industrial uses, a chemical process needs to work on a concentrated solution that ranges from 10-20 wt percentage. And current oxidation methods are unable to achieve a yield that is high enough to fulfill commercial requirements.. Sponsored solution from CWC Labs: This heavy metals test kit allows you to test almost anything for 20+ heavy metals and nutritive minerals, including lead, mercury, arsenic, cadmium, aluminum and more. You can test your own hair, vitamins, well water, garden soil, superfoods, pet hair, beverages and other samples (no blood or urine). ISO accredited laboratory using ICP-MS (mass spec) analysis with parts per billion sensitivity. Learn more here.. In earlier experiments involving the minimum 10 wt percent concentrated solution of HMF, only 30 percent of that biomatter got oxidized into FDCA. This is a huge drop in efficiency compared to its effectiveness on 20 percent.. Furthermore, many of the formyl groups present in the ...

Ελληνική Γαστροεντερολογική Εταιρία. Annals of Gastroenterology.Τμήμα περιοδικού.Επιστημονικό άρθρο.Άρθρο.2007 . Creators: Konstantellou, Helen, Argyros, N., Sambani, Constantina, Kottaras, G., Hereti, Irini, Choremi, Helen, Cheracakis, P., Christodoulou, Martha.A,â-lipoproteinemia is an extremely rare genetic disorder of lipoprotein metabolism, associated with various biochemical abnormalities combined with clinical malabsorption. The aim of this study was to provide information concerning the cytogenetic and HLA patterns of three patients with A,â-lipoproteinemia, as well as to see if there is any correlation between specific HLA type and clinical course of the disease.The study included seven persons. There were 3 patients (a girl and two boys aged 32, 30 and 22 years respectively) and their 4 healthy, first-degree relatives (two parents, a brother and a sister). Cytogenetic studies wereperformed on PHA stimulated peripheral

For The Complete Final Report CLICK HERE. Sustaining Health Sustaining Housing is an evaluation study of our Jarvis Program investigating how PHAs, who have accessed support services, view the impact of these services on their housing experiences and their overall health. Thirty five residents (43%) were recruited and interviewed by the Peer Research Assistants for the quantitative study; fifteen went on to participate in focus groups.. The study reveals that supportive services at Jarvis have a positive impact on the lives of PHAs. Eighty nine percent (89%) indicated that it is important to have support services within the building. The majority of participants (66%) reported that they were satisfied with Fife House services and knowing that they are available enhanced a sense of security and reduced isolation.. …you know with this disease youre going to have a turn… And anyone who I know whos…had a real hard time and struggling has felt very supported and very cared for by ...

Beginning in January 2017, ATSDR will begin to take steps to streamline our website. As a result, Public Health Assessments (PHAs) and Health Consultations (HCs) preceding 2004 will no longer be available on our website. A list of documents that have been removed can be found in the Archive documents pdf below. To access PHAs and HCs published before October 1, 2004, please send a request to the ATSDR Records Center by phone: 770-488-0707 or email: [email protected]. We apologize for any inconvenience ...

PDM Somthin ElseAMAA #508025 • 1/2 Maine-Anjou GOET I BELIEVEsire. GETCHA SOME ET PHAF THCASR DREAMS COME TRUE 301Z COWANS ALI 4Mdam. PM MS MOLLYSUPER GIRL S633 owned with M LAZY HEART RANCH/GRISWOLD CATTLE We think hes an awfully special animal to come from a flush that has sold every steer for 20k plus, and yet is b

Shrilk Biodegradable Plastic on Wyss Institute | Wyss Institute researchers have developed a fully degradable bioplastic by isolating a material called…

Once we will establish the production process and describe phiscal properties of the novel materials, we will start biological tests. The novel functionalised materials will be characterized in vitro for their biocompatibility with chosen cell lines appropriate for their future applications. In parallel we will conduct test in order to descibe antibacterial properties of the novel functionalised PHAs. ...

**Do Not Post to this Topic* Start a new topic, please. The Rules of Induction from www.atkins.com/articles/atkins-phases/phas e-one/the-rules-of-induction This phase of the , team1783board