Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polym
Polyhydroxyalkanoate (PHA) is a versatile class of biodegradable and biocompatible biopolyesters accumulated by many bacteria as intracellular carbon storage compound. The largest subclass consists of medium-chain-length (mcl) PHA which has a large potential in medical applications where flexible or elastomeric materials are required. Different extraction and purification methods for mcl-PHA are known to result in variations of polymer purities. In this study it was assessed whether this difference in quality may increase risk of failure of the implant material by enhanced colonization by Gram-negative and Gram-positive model pathogens (Staphylococcus aureus and Escherichia coli curli). Two types of mcl-PHA copolymers, poly(3-hydroxyoctanoate) (PHO) and the less known poly(3-hydroxyundecanoate) (PHUA), were evaluated for an impact of monomeric unit composition and degree of polymer purity on colonization by the model pathogens. It was found that film formation by bacteria on purified PHO and PHUA
A method of recovering polyhydroxyalkanoates (PHAs) from municipal waste sludge includes: (a) measuring a solid content in the municipal waste sludge in terms of a volatile suspended solid in the mun
As the only kind of naturally-occurring biopolyester synthesized by various microorganisms, polyhydroxyalkanoate (PHA) shows a great market potential in packaging, fiber, biomedical, and other fields due to its biodegradablity, biocompatibility, and renewability. However, the inherent defects of scl-PHA with low 3HV or 4HB content, such as high stereoregularity, slow crystallization rate, and particularly the phenomena of formation of large-size spherulites and secondary crystallization, restrict the processing and stability of scl-PHA, as well as the application of its products. Many efforts have focused on the modification of scl-PHA to improve the mechanical properties and the applicability of obtained scl-PHA products. The modification of structure and property together with the potential applications of scl-PHA are covered in this review to give a comprehensive knowledge on the modification and processing of scl-PHA, including the effects of physical blending, chemical structure design, and
SIRIM consortium researchers developed the process technology in 2006 to produce polyhydroxyalkanoates (PHA) polyesters from palm oil wastes. (Image courtesy of SIRIM Berhad.)
Misra, Superb K., Nazhat, Showan N., Torbati, M.M., Silva, S.R.P., Roy, Ipsita and Boccaccini, Aldo R. (2006) From bacteria to tissue engineering applications-usage of polyhydroxyalkanoates for biomedical applications. In: Proceedings of the International Conference on the Design of Biomaterials, 08 - 11 Dec 2006, Kanpur, India. Full text not available from this repository ...
A single-nucleotide substitution in phasin gene leads to enhanced accumulation of polyhydroxyalkanoate (PHA) in Escherichia coli harboring Aeromonas caviae PHA biosynthetic operonA single-nucleotide substitution in phasin gene leads to enhanced accumulation of polyhydroxyalkanoate (PHA) in Escherichia coli harboring Aeromonas caviae PHA biosynthetic operon ...
The investigation and development of plastics synthesized by bacteria is receiving great attention also due to the raising petroleum prices and many environmental concerns related to plastic pollution. Recent results and studies of the properties and various applications of bio-based plastics are presented in this volume. Polyhydroxyalkanoates (PHAs), a biodegradable compound, is treated in several chapters: PHAs as energy and intracellular carbon storage compounds, the metabolic engineering of PHA producers, the development of tailor-made PHAs including uncommon monomers, microbial PHA production from waste raw materials, PHA polyesters produced by both wild-type and recombinant bacteria and the production of medium-chain-length PHAs in pseudomonads. Further microbial plastics discussed are lactic acid and its polymer polylactic acid (PLA), succinic acid and its polymer polybutylene succinate (PBS), ethylene from ethanol and its polymer polyethylene, 1,3-propandiol as well as poly(p-phenylene) ...
By Renard, E Langlois, V; Guerin, P Polyhydroxyalkanoates (PHAs) form an enlarged family of biopolyesters, which are biocompatible, biodegradable and non- toxic. Polyhydroxyalkanoates biodegradation corresponds to a hydrolysis involving endo- or exo-enzymatic systems in the breaking cleavage of esters bonds. This type of degradation is needed for environmental applications. In the case of therapeutic and biomedical uses, a simple hydrolysis is required. Hydrolytic degradation of PHAs is not evident as shown on poly(3- hydroxyoctanoate) (PHO) and is depending on the structure of the polyester and more particularly on the nature of the side chains. In some cases, blending with others polymers has decreased PHAs crystallinity and has made easier hydrolysis. Another route consists in the preparation of unsaturated PHAs which can be chemically modified. Pendant double bonds have been turned into carboxylic, hydroxyl or epoxy groups. Moreover these reactive functions were used for grafting oligomers ...
In this work, sludge was submitted to aerobic dynamic substrate feeding. Two sequencing batch reactors were operated, with acetate or propionate as carbon substrates. When acetate was used the system only produced a homopolymer of polyhydroxybutyrate (PHB). In order to maximize the PHB production, tests with different concentrations of acetate and ammonia were preformed. The best results (67.2% of PHB by cell dry weight) were obtained for 0.7 Nmmol/l of ammonia and 180 Cmmol/l of acetate. The PHB cell content was further improved by pupulse addition of substrate, three times 60 Cmmol/l of acetate, reaching a value of 78.5%. Propionate can be used as a precursor for hydroxyvalerate. In conjunction with other substrates, it allows for the formation of copolymers, which present better processing properties on commercial applications. Tests with different concentrations of propionate and ammonia were performed. Under the operating conditions used, the maximum PHA accumulated inside cells was 34.8%, ...
Introduction. Polyhydroxyalkanoates (PHA) are bio-polymeric materials that exhibit excellent biodegradability and biocompatibility. These biodegradable polymers possess excellent physico-mechanical properties resulting in their current increase in diverse applications. In fact they are commonly utilized in various biomedical applications spanning from drug delivery devices to tissue engineering scaffoldings (Francis, 2011).. These polymers are accumulated in vivo as cellular inclusions and serve as carbon source reserves in several bacterial species (Xue et al., 2010; Hofer et al., 2011; Gumel et al., 2012b). Among the most extensively studied bacterial species capable of accumulating these biopolymers are those belonging to genus Pseudomonas reported to accumulate intracellular PHA under limited nutrient conditions and abundant carbon source such as fatty acids (Annuar et al., 2007; Gumel, et al., 2013).. For a successful process optimization, acquisition of process kinetics information ...
Production of polyhydroxyalkanoates (PHAs) in activated sludge treating wastewater represents an economical and environmental promising alternative to pure culture fermentations. A process for production of PHA from a paper mill wastewater was examined at laboratory scale. The three stage process examined consisted of acidogenic fermentation to convert wastewater organic matter to volatile fatty acids (VFAs), an activated sludge system operating under feast/famine conditions to enrich for PHA producing organisms and accumulation of PHA in batch experiments. After fermentation of the wastewater, 74% of the soluble COD was present as VFA (acetate, propionate, butyrate and valerate) and the resulting PHA after batch accumulation consisted of 31-47 mol% hydroxybutyrate and 53-69 mol% hydroxyvalerate. The maximum PHA content achieved was 48% of the sludge dry weight and the three stage process exhibited a potential to produce 0.11 kg of PHA per kg of influent COD treated ...
The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved p
Polyhydroxyalkanoates (PHA) [1] are natural thermoplastics synthesized by many microorganisms as an intracellular storage material under unbalanced conditions of growth. As a substitute, to synthetic petrochemical polymers as well as to fulfill the growing demand of environmentally friendly plastics, production of PHA is under intensive studies [2]. PHA belong to a family of fully biodegradable polymers with no toxicity which can also be used for medical applications [3, 4]. PHA can be completely bio-degraded into oligomers and monomers and then to CO2 and water, all are environmentally benign [5-7]. Some PHA have been developed for various applications including bioplastics for packaging, implant materials, biofuels and fine chemicals [8-10].. On the basis of monomer structures, PHA are divided into short chain length (scl) polymers consisting of monomers of C3-C5 carbon atoms, medium chain length (mcl) polymers consisting of monomers of C6-C16 carbons atoms, as well as PHA copolymers ...
Polyhydroxyalkanoates are a class of over 80 polyesters synthesized by more than 300 microorganisms that exhibit material properties similar to those of petrochemical plastics (Lee, 1999; Lee, 1996). Polyhydroxybutyrate (PHB), the most prevalent PHA, has been of particular interest in commercial and medical applications because of its high tensile strength, biodegradability, and biocompatibility with human tissues (Lee, 1996b; Abe, 1992; Williams 2002). Concerns with the increasingly limited supply of fossil fuels and public anxiety over landfills oversaturated with non-biodegradable materials have also stimulated bioplastic research (Moran, 2006; Ojumu, 2004). PHA research has increased in an effort to understand its potential. It has been used for specialized medical applications, including bone fixation, drug delivery systems and degradable sutures (Knowles, 1993: Kose, 2004; Holmes, 1985: Chen, 2005). ...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
Bio-on and SECI Spa, part of Gruppo Industriale Maccaferri holding, signed an agreement to build the worlds first facility for the production of PHAs bioplastic from biodiesel production co-products, namely glycerol. The two companies will work together to build a production site with a 5 thousand tons/year output, expandable to 10 thousand tons/year. Requiring a 55…
Single-use plastic is a growing problem for our planet. Now, one student has created a bioplastic using an unconventional material - fish skins and scales.
مقدمه: فیتاز، فیتیک‌اسید را هیدرولیز می‌کند و دسترسی زیستی فسفر و دیگر مواد معدنی مغذی را برای حیوانات تک‌معده‌ای افزایش می‌دهد.بنابراین، به‌شکل افزودنی غذایی مهم استفاده می‌شود. مواد و روش‏‏ها: هدف مطالعه حاضر، جداسازی باکتری‌‌های تولیدکننده فیتاز از مزرعه لوبیای شوشتر، جنوب‌غرب ایران، با استفاده از محیط غربال‌گری فیتاز (PSM) و بهینه‌سازی رشد و شرایط تولید آنزیم با بهترین جدایه است. نتایج: بهترین جدایه، سیتروباکتر فارمری سویه phas32 شناسایی شد. دمای 30 درجه سانتی‌گراد، اسیدیته 7، 25/0 درصد فیتیک‌اسید و 48 ساعت انکوباسیون، شرایط بهینه‌سازی‌شده برای
Fe-Zr oxides with different Zr/Fe molar ratios were prepared by a co-precipitation method and used as catalysts for the vapor phas... ...
The synthesis and degradation of polyhydroxyalkanoates (PHAs), the storage polymer of many bacteria, is linked to the operation of central carbon metabolism. To rationalize the impact of PHA accumulation on central carbon metabolism of the prototype bacterium Pseudomonas putida, we have revisited PHA production in quantitative physiology experiments in the wild-type strain vs. a PHA negative mutant growing under low nitrogen conditions. When octanoic acid was used as PHA precursor and as carbon and energy source, we have detected higher intracellular flux via acetyl-CoA in the mutant strain than in the wild type, which correlates with the stimulation of the TCA cycle and glyoxylate shunt observed on the transcriptional level. The mutant defective in carbon and energy storage spills the additional resources, releasing CO2 instead of generating biomass. Hence, P. putida operates the metabolic network to optimally exploit available resources and channels excess carbon and energy to storage via PHA, ...
de Eugenio, L.I., García, P., Luengo, J.M., Sanz, J., San Román, J., García, J.L., and Prieto, M.A. (2007) Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: characterization of a paradigmatic enzyme. J Biol Chem 282: 4951-4962 ...
1.Chen GQ, Wu Q. 2005. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials. 26:6565-6578 2. Doi Y, Kunioka M, Nakamura Y, Soga K. 1986. Nuclear magnetic resonance studies on poly(B-hydroxybutyrate) and a copolyester of B-hydroxybutyrate and B-hydroxyvalerate isolated from Alcaligenes eutrophus H16. Macromolecules. 19:2860-2864 3. Endy D. 2005. Foundations for engineering biology. Nature. 438(7067):449-53 4. International Genetically Engineered Machines competition. 15 Jun 2008. 26 Jul 2008. ,http://igem.org, 5. Holmes PA. 1985. Applications of PHB - a microbially produced biodegradable thermoplastic. Physics in technology. 16:32-36 6. Kang Z, Wang Q, and H Zhang. 2008. Construction of a stress-induced system in Escherichia coli for efficient polyhydroxyalkanoates production. Biotechnological Products and Process Engineering. 79:203-208 7. Knight TF. 2003. Idempotent Vector Design for Standard Assembly of BioBricks. Tech. rep., MIT Synthetic Biology Working ...
AB - The polyhydroxyalkanoates (PHAs) are microbially-produced biopolymers that could potentially be used as sustainable alternatives to oil-derived plastics. However, PHAs are currently more expensive to produce than oil-derived plastics. Therefore, more efficient production processes would be desirable. Cell-free metabolic engineering strategies have already been used to optimise several biosynthetic pathways and we envisioned that cell-free strategies could be used for optimising PHAs biosynthetic pathways. To this end, we developed several Escherichia coli cell-free systems for in vitro prototyping PHAs biosynthetic operons, and also for screening relevant metabolite recycling enzymes. Furthermore, we customised our cell-free reactions through the addition of whey permeate, an industrial waste that has been previously used to optimise in vivo PHAs production. We found that the inclusion of an optimal concentration of whey permeate enhanced relative cell-free GFPmut3b production by ∼50%. In ...
A new bacterium, designated as strain TE9 was isolated from a microbial mat in French Polynesia and was studied for its ability to synthesize medium chain length poly-beta-hydroxyalkanoates (mcl PHAs) during cultivation on cosmetics co-products. The composition of PHAs was analysed by coupled gas chromatography mass spectroscopy (GC/MS), nuclear magnetic resonance (NMR) and Fourier Transform InfraRed (FTIR) spectroscopy. PHAs were composed of C6-C14 3-hydroxyacids monomers, with a predominance of 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD) and 3-hydroxydodecanoate (3HDD). Differential scanning calorimetry (DSC) experiments allowed the characterization of elastomeric materials with a melting point T(m) near 50 degrees C, enthalpy of fusion DeltaH(m) from 27 to 32 J/g, and glass transition temperature T(g) of -43 degrees C. Molecular weights ranged from 175,000 to 358,000 g/mol. On the basis of the phenotypical features and genotypic investigations, strain TE9 was assigned to the Pseudomonas genus
In California, researchers at the University of California, Berkeley have coaxed Escherichia coli to use fluorinated building blocks to make new organofluorine target molecules.. Led by with Michelle C.Y. Chan, the group able to introduce genes to code for enzymes that use fluorine-containing derivatives of their normal substrates. The researchers also introduced a gene for an enzyme used by many bacteria to make polyhydroxyalkanoates (PHAs), a biodegradable bioplastic. The fluorinated versions of PHA were less brittle than conventional PHAs, opening up new applications. The researchers also hope to produce small fluorinated molecules in living cells for pharmaceuticals. ...
Abstract: Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is one of the components of polyhydroxyalkanoates (PHAs) and some of its mechanical properties have been shown to improve over poly (3-hydroxybutyrate) (PHB) and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The investigation of PHBHHx microspheres as a drug delivery system was prepared by emulsion-solvent evaporation method for the sustained release of anti-cancer drug 5-fluorouracil (5-FU) and cyclosporin A (CsA). The mean diameter of the PHBHHx microspheres ranged from 5.24 to 22.10 μm dependent on the different processing parameters. The PHBHHx concentration, emulsifier concentration, anti-cancer drug dosage, and agitation speed, were optimized according to the encapsulation efficiency of 4% PHBHHx, 0.5% SDS, 10 mg anti-cancer drug, and 500 rpm. Under optimized conditions, the encapsulation efficiency of 5-FU and CsA microspheres were 7.19% and 96.44%, respectively. The morphologies of scanning electron microscope ...
23. The norbornene polymer according to claim 8, wherein the norbornene polymer is a copolymer further comprising a polar norbornene repeating unit represented by Formula 3 and a nonpolar norbornene repeating unit represented by Formula 4:wherein each m is independently an integer of 0 to 4,at least one of R1a, R2a, R3a, and R4a is a polar functional group containing at least one atom selected from the group consisting of oxygen, nitrogen, phosphorus, sulfur, silicon, and boron, andR1a, R2a, R3a, and R4a that are not the polar functional group are each independently hydrogen; halogen; linear or branched alkyl, alkenyl, or vinyl having 1 to 20 carbon atoms; cycloalkyl that is unsubstituted or substituted with hydrocarbon and has 5 to 12 carbon atoms; aryl that is unsubstituted or substituted with hydrocarbon and has 6 to 40 carbon atoms; aralkyl that is unsubstituted or substituted with hydrocarbon and has 7 to 15 carbon atoms; alkynyl that has 2 to 20 carbon atoms; linear or branched haloalkyl, ...
The bio-transformation of organic crops, such as corn, has led to a range of polyesters such as poly lactic acid (PLA) and polyhydroxyalkanoates (PHA). ). Applications of these polyesters could include paper coatings, wood product finishing, adhesives, and waterborne coating technologies. Researchers at UNH are preparing waterborne hybrid vinylic polyester latices through a miniemulsion polymerization process ...
Some of the top companies like Asta Real and Fuji Health have doubled its production capacity recently owing to the increasing demand of astaxanthin.. Lutein, a carotenoid extracted from algae, is capable of preventing the onset of Age-related Macular Degeneration (AMD). The global market value of lutein was around $233 million in 2010 and is expected to reach $309 million by 2018.. Algae are also widely used in the production of enzymes and toxins which, in general, have a product values in the range of US $4000-8000/kg. Algae are also used in cosmetics industry as thickening agents, water-binding agents and antioxidants.. Algae based added value commodities such as lactic acid, polyhydroxyalkanoates and butanol price ranges from US $1300 to US $7000/tonne.. Learn more on the business opportunities in algae non-fuel products from Comprehensive Report on Attractive Algae Product Opportunities. ...
The results of the stereolithographic habitat construction process of Cesaretti et al. [11] can also be accomplished with a three-dimensional printer that extrudes plastic media using fused deposition modelling (FDM). A comparison of the different additive manufacturing methods and possible feedstocks is included in [49], which explains why FDM is preferred over stereolithography for space applications. To determine the existence of launch mass savings for three-dimensional printed habitats from biological processes, this section examines the extraterrestrial manufacture of feasible three-dimensional printer raw materials like polyhydroxyalkanoate (PHA) biopolymers, of which polyhydroxybutyrates (PHBs) are an example. A contrast of habitat structural properties when using either salt-MgO or biopolymers as printer media is beyond the scope of this paper.. The biomanufacture of PHAs is reviewed in [122-125], and Lu et al. [123] include a list of organisms known to synthesize PHAs. It has been ...
Its time to talk about something that almost everyone can use without being worried about breaking out. AHAs and BHAs are fantastic chemical exfoliants but weve heard way too many cases of people breaking out or purging periods being too long. Fear no more ladies for its time to welcome PHAs into our lives. So, what
A process for producing a toner for developing electrostatic images is disclosed. A polymerizable monomer composition is first prepared and dispersed in an aqueous medium by the action of high shear force to produce particles. The polymerizable monomer is then polymerized in a container having a jacket. The liquid temperature inside the container is controlled by feeding a high-pressure vapor into the jacket while adjusting the degree of vacuum inside the jacket. The production process can achieve a superior temperature response, can keep the reaction system at a constant temperature with ease and can realize shortened production time and reduced production cost.
Metabolix biopolymers are based on polyhydroxyalkanoate polymers (PHAs) and are made by fermentation.. using renewable carbon based feedstocks, making them 100% biobased in neat form.. ...
Large-scale production and purification of recombinant proteins by cell cultures represent a key-area in manufacturing field. The production process still has several drawbacks affecting cost/efficiency. In this work, three modular systems were designed to overcome some of these bottlenecks. A library of self-inducible promoters was built and characterized to start the peptide production at a desired culture density, without expensive inducer molecules. Two standard integrative vectors were realized to insert BioBrick parts in user-defined positions of E. coli or S. cerevisiae genome, to ensure genetic stability without using selection markers. Finally, two promising techniques were combined for an "in-cell" protein purification: PolyHydroxyAlkanoate (PHA) granules were used as a substrate for PHA-binding peptides (Phasins) fused to the target protein, thus replacing affinity resins/columns and tags, while a pH-based self-cleaving peptide (Intein) was used instead of a protease cleavage site. ...
Large-scale production and purification of recombinant proteins by cell cultures represent a key-area in manufacturing field. The production process still has several drawbacks affecting cost/efficiency. In this work, three modular systems were designed to overcome some of these bottlenecks. A library of self-inducible promoters was built and characterized to start the peptide production at a desired culture density, without expensive inducer molecules. Two standard integrative vectors were realized to insert BioBrick parts in user-defined positions of E. coli or S. cerevisiae genome, to ensure genetic stability without using selection markers. Finally, two promising techniques were combined for an "in-cell" protein purification: PolyHydroxyAlkanoate (PHA) granules were used as a substrate for PHA-binding peptides (Phasins) fused to the target protein, thus replacing affinity resins/columns and tags, while a pH-based self-cleaving peptide (Intein) was used instead of a protease cleavage site. ...
Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2020 Elsevier B.V. We use cookies to help provide and enhance our service and tailor content. By continuing you agree to the use of cookies. Log in to Pure. ...
If he chooses to use only Styrofoam items (which weighs much less than PLA and plastic items) - in one year, he will have consumed 191.11 kWh of energy, released 95.81 pounds of CO2 and used 254.59 gallons of water. Since the average household uses 29.19 kWh of energy per day, this would be enough energy to power a household for 6.55 days ...
chains in the Genus database with same CATH superfamily 3C1Y A; 4Z6D A; 1KFD A; 5D49 A; 4I2I A; 3MR2 A; 4YMO A; 3GII A; 2BPF A; 1T7P A; 4PHA A; 4RNN A; 4QWC A; 4CCH A; 1ARO P; 1ZQK A; 5KFB A; 3MFH A; 5HP4 A; 5KT3 A; 1Z00 B; 4YVZ A; 3KHH A; 3THV A; 3RBE A; 3RJK A; 2J6T A; 1SKR A; 1MQ3 A; 4YXM A; 1U47 A; 5J2H A; 4Q45 A; 2H5X A; 4UAZ A; 2XY6 A; 3LDA A; 5KFE A; 4J9L A; 5DB9 A; 3KHR A; 2Y1J A; 2MUT B; 9ICP A; 3C1Z A; 2GWS A; 3MBY A; 3BDP A; 4FBU A; 4DSL A; 1SL2 A; 5CP2 A; 4DQP A; 2DFL A; 2VA8 A; 3BQ0 A; 5CHG A; 4XRH A; 1SL1 A; 3RJG A; 3OHA A; 4JK1 A; 9ICT A; 2I1Q A; 4C8O A; 3RRH A; 2ASD A; 1QSY A; 2W8L A; 2R8H A; 2Y1I A; 3HPO A; 1BPY A; 2PFO A; 2ZUC A; 3V7J A; 2HHX A; 2KZZ A; 1QLN A; 4UB5 A; 1K1Q A; 3V7K A; 1T4G A; 4ZH4 A; 5J2K A; 2R8I A; 3UXP A; 4PPX A; 4MF2 A; 4O3O A; 3ETL A; 4F91 B; 5J0R A; 1TK0 A; 4RNO A; 4RQ0 A; 2MUT A; 4TUR A; 3TFR A; 1ZQA A; 1D9F A; 4P4M A; 3MGI A; 3ZDA A; 4QZA A; 4GC6 A; 3FYH A; 5KFC A; 4ECZ A; 3T3F A; 3C5G A; 2PI5 A; 4YFK A; 9ICK A; 7ICV A; 4UB3 A; 2NRZ A; 3C23 A; 4NLG A; ...
He is clean necked, big hipped and has a killer look when you study him from the profile.. Hair like a carrier, but is THF and PHAF to breed to your carrier cows.. Winning is in his pedigree, as his dam is the full sister to the dam of the 2015 NWSS grand champion steer.. ...
Every month, a womans body prepares itself for the possibility of conception and pregnancy by going through a menstrual cycle. During the menses phas...
Can you name the Phar 401 Druglist Test your knowledge on this science quiz to see how you do and compare your score to others. Quiz by mpchiang
Visiongain publishes insightful market-led reports that will help you understand future opportunities from changes in technologies, materials & regulations.
Visiongain publishes insightful market-led reports that will help you understand future opportunities from changes in technologies, materials & regulations.
Polyhydroxybutyrate (PHB) is one of the polyhydroxyalkanoates (PHAs) which has biodegradable and biocompatible properties. They are adopted in the biomedical field, in, for example, medical implants and drug delivery carriers. This study seeks to promote the production of PHB by Vibrio sp. BM-1, isolated from a marine environment by improving constituents of medium and implementing an appropriate fermentation strategy. This study successfully developed a glycerol-yeast extract-tryptone (GYT) medium that can facilitate the growth of Vibrio sp. BM-1 and lead to the production of 1.4 g/L PHB at 20 h cultivation. This study also shows that 1.57 g/L PHB concentration and 16% PHB content were achieved, respectively, when Vibrio sp. BM-1 was cultivated with MS-GYT medium (mineral salts-supplemented GYT medium) for 12 h. Both cell dry weight (CDW) and residual CDW remained constant at around 8.2 g/L and 8.0 g/L after the 12 h of cultivation, until the end of the experiment. However, both 16% of PHB content and
phdthesis{de533308-cdd5-4693-8db6-38f644972c19, abstract = {This thesis presents studies on moderately halophilic bacteria belonging to the family Halomonadaceae that accumulate polyhydroxybutyrate (PHB) in high amounts when grown under conditions of nitrogen limitation. PHB is the simplest homopolymer belonging to the class of polyhydroxyalkanoates (PHAs), the biopolyesters that can biodegraded in the environment to carbon dioxide and water and are hence attractive substitutes for fossil-derived plastics. ,br/,,br, Halomonas andesensis was isolated from a hypersaline lake in the Andean region of Bolivia, and was identified as a novel species by phenotypic and genotypic characterization. The organism utilizes different carbon sources such as acetate, L-arabinose, cellobiose, D-fructose, galactose, D-gluconic acid, D-glucose, L-lactic acid, maltose, myo-inositol, propionic acid, raffinose, L-rhamnose, sucrose, starch and D-xylose, respectively, for growth. ,br/,,br, Halomonas boliviensis was also ...
The influence of leukemic serum on phytohemagglutinin-P (PHA-P)-induced lymphocyte transformation was studied. Twenty of 22 pretreatment sera collected at diagnosis inhibited the transformation of normal lymphocytes stimulated with PHA-P by more than 50%. In these experiments with PHA-P, kinetic studies of transformation demonstrated that leukemic serum inhibited rather than delayed transformation. The inhibition of transformation by PHA-P was assumed to be due to binding of PHA by components of leukemic serum because the inhibition could be overcome by an increase in the PHA concentration. A biologic assay of the PHA content of lysates of lymphocytes preincubated with PHA and leukemic serum further substantiated the PHA-binding mechanism of leukemic serum. Whether the differences observed in the binding capacity of normal and leukemic serum are qualitative or quantitative remains to be determined.. ...
View the basic SGB.V stock chart on Yahoo Finance. Change the date range, chart type and compare SOLEGEAR BIOPLASTIC TECH INC against other companies.
A complex comprises organoborane and polyamine. The polyamine is the reaction product of a diprimary amine-terminated material and a material having at least two groups reactive with primary amine. The complexes are useful in systems for initiating the polymerization of acrylic monomer, which systems further include a material reactive with amine. Polymerizable acrylic monomer compositions useful as adhesives can be prepared.