Page contains details about N-octyl-N-quatenary chitosan cross-linked with low-molecular weight polyethylenimine/plasmid encoding enhanced green fluorescent protein complex nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Amphiphilic graft copolymer based on poly(styrene-co-maleic anhydride) with low molecular weight polyethylenimine for efficient gene delivery Xiaopin Duan,1,2 Jisheng Xiao,2 Qi Yin,2 Zhiwen Zhang,2 Shirui Mao,1 Yaping Li21School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 2Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, ChinaBackground and methods: A new amphiphilic comb-shaped copolymer (SP) was synthesized by conjugating poly(styrene-co-maleic anhydride) with low molecular weight polyethyleneimine for gene delivery. Fourier transform infrared spectrum, 1H nuclear magnetic resonance, and gel permeation chromatography were used to characterize the graft copolymer.Results: The buffering capability of SP was similar to that of polyethyleneimine within the endosomal pH range. The copolymer could condense DNA effectively to form complexes with a positive charge (13–30 mV) and a small particle size (130–200 nm) at N/P ratios
Enhancement of DNA delivery efficiency has been improved by several physical and chemical approaches in the past decade. However, the application of those methods is hampered by large-scaled configurations. To augment gene delivery efficiency with scalable settings, the potential of resonant acoustic fields (RAF) to facilitate DNA delivery was explored. We reasoned that, driven by the primary acoustic radiation force, suspended cells agglomerated on the pressure nodal planes first and formed cell bands. Nanometer-sized DNA vectors, circulated between nodal planes by acoustic microstreaming, then used the pre-formed cell bands as the nucleating sites to attach on. As a result, the encounter opportunity between DNA vectors and target cells was increased and further enhanced the gene delivery efficiency. In this talk, delivery efficacy of DNA ferried by retroviral or nonviral vector was examined applying RAF. For the viral vector part, our results showed that mega-hertz RAF brought K562 erythroleukemia
Development of efficient antibacterial agents is critical for human health. In the present study, we investigated the antibacterial activity of polyethyleneimine (PEI)-capped silver nanoclusters (PEI-AgNCs), based on the fact that nanoclusters normally have higher surface-to-volume ratios than traditional nanomaterials and PEI itself has a strong antimicrobial capacity. We synthesized stable silver nanoclusters by altering PEI molecular weight from 0.6 kDa to 25 kDa and characterized them by UV-Vis absorption and fluorescence spectroscopy and high resolution transmission electron microscopy. The sizes of AgNCs were around 2 nm in diameter and were little influenced by the molecular weight of PEIs. The antibacterial abilities of the four PEI-AgNCs were explored on agar plate and in liquid systems. Our results revealed that the antibacterial activity of PEI-AgNCs is excellent and the reduction of PEI molecular weight could result in the increased antibacterial capacity of PEI-AgNCs. Such proposed new
The effect of the polycation polyethyleneimine (PEI) on the permeability properties of the Gram-negative bacterial outer membrane was investigated using Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium as target organisms. At concentrations of less than 20 μg ml-1, PEI increased the bacterial uptake of 1-N-phenylnaphthylamine, which is a hydrophobic probe whose quantum yield is greatly increased in a lipid environment, indicating increased hydrophobic permeation of the outer membrane by PEI. The effect of PEI was comparable to that brought about by the well-known permeabilizer EDTA. Permeabilization by PEI was retarded but not completely inhibited by millimolar concentrations of MgCl2. PEI also increased the susceptibility of the test species to the hydrophobic antibiotics clindamycin, erythromycin, fucidin, novobiocin and rifampicin, without being directly bactericidal. PEI sensitized the bacteria to the lytic action of the detergent SDS in assays where the bacteria were pretreated
A novel, efficient DNA delivery and transfection agent has been developed by chemically attaching short chain polyethyleneimine (PEI) molecules (M.W. = 423 and 1800) to the surface of composite iron oxide nanoparticles (250 nm in diameter). These particles are not only capable of delivering DNA but also can
Fe3O4 magnetic nanoparticles (MNPs), as one of the most intensively researched NPs, have a range of applications in cancer treatments. In current research, we have focused on the influences of MNPs on cancer cells. We chose polyethyleneimine (PEI) coated MNPs (PEI-MNPs) as a model and they are colloidally st
Carbon dots (CDs) have attracted great attention in a broad range of applications due to its unique optical properties, high biocompatibility and property adjustability. However, developed CDs can be rarely used as effective gene vectors up to now. In this work, a fluorine-doping strategy was provided to prepare a novel fluorine-doped CDs (F-CDs) using fluorine-containing aromatic compound as fluorine source and using branched polyethyleneimine (PEI) to furnish positive charge sites. The as-synthesized F-CDs achieves dramatic gene transfection efficiency as well as low cytotoxicity in commonly used cell lines at low weight ratio. It is worthy to point out that this F-CDs is superior efficiency and better biocompatibility compared to some widely used commercial reagents such as branched 25k Da PEI and Lipofectamine 2000. At weight ratio (transfection reagent/EGFP plasmid) of 2, the transfection efficiency of F-CDs achieves about 92% in HEK 293T cells, 85% in COS-7 cells, 81% in A549 cells, 73% in ...
Ovarian cancer remains the most lethal gynaecological cancer mainly due to the lack of reliable biomarkers and eventual development of chemo-resistance. This emphasizes the need for better therapies. Ovarian cancer is considered as an immunogenic tumour and adoptive immunotherapy is a promising treatment strategy. However, co-inhibitory molecules such as programmed death-ligand 1 (PD-L1), highly expressed on ovarian cancer cells interacts with its receptor, programmed death-1 (PD-1), expressed on T cells, causing immunosuppression. The aim of this Ph.D. was to 1) develop more efficient and targeted gene delivery agents by functionalizing poly(ethylenimine) (PEI) with various hydrophobic groups and folic acid (FA) targeting ligand, 2) deliver PD-L1 small interfering RNA (siRNA) or short hairpin RNA (shRNA) into ovarian cancer cells to block PD-1/PD-L1 interactions and 3) to study how T cell function and anti-tumour activity are affected as a consequence of PD-L1 knockdown. 4) In addition, ...
Bone marrow derived human mesenchymal stem cells (hMSCs) show promising potential in regeneration of defective tissue. Recently, gene silencing strategies using microRNAs (miR) emerged with the aim to expand the therapeutic potential of hMSCs. However, researchers are still searching for effective miR delivery methods for clinical applications. Therefore, we aimed to develop a technique to efficiently deliver miR into hMSCs with the help of a magnetic non-viral vector based on cationic polymer polyethylenimine (PEI) bound to iron oxide magnetic nanoparticles (MNP). We tested different magnetic complex compositions and determined uptake efficiency and cytotoxicity by flow cytometry. Additionally, we monitored the release, processing and functionality of delivered miR-335 with confocal laser scanning microscopy, real-time PCR and live cell imaging, respectively. On this basis, we established parameters for construction of magnetic non-viral vectors with optimized uptake efficiency (~75%) and moderate
Linear polyethylenimines (PEIs) contain all secondary amines, in contrast to branched PEIs which contain primary, secondary and tertiary amino groups.
Novel bio-reducible polymer compositions which combine the ability to act as a CXCR4 antagonist and simultaneously provide enhanced gene delivery through the formation of inert nanocarrier polyplexes have been developed. As would be expected for biodegradable polymers, the cytotoxicity of these nanocarrier compositions are very low with measured IC50 values 50 to 100 times higher than conventional polyethylenimines (PEI) controls. Significantly, the known CXCR4 antagonist drug AMD3100 has successfully been incorporated into the polymer composition and shown to retain activity comparable or better than the drug alone with or without the formation of a nanocarrier polyplexes. Successful gene delivery by the nanocarrier polyplexes has been demonstrated through fluorescence imaging studies and measurements of transfected gene expression levels. Preliminary results of two different animal studies confirm the CXCR4 antagonist response of this novel composition for both lung cancer metastasis and ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
The successful delivery of therapeutic genes to the designated target cells and their availability at the intracellular site of action are crucial requirements for successful gene therapy. Nonviral gene delivery is currently a subject of increasing attention because of its relative safety and simplicity of use; however, its use is still far from being ideal because of its comparatively low efficiency. Most of the currently available nonviral gene vectors rely on two main components, cationic lipids and cationic polymers, and a variety of functional devices can be added to further optimize the systems. The design of these functional devices depends mainly on our understanding of the mechanisms involved in the cellular uptake and intracellular disposition of the therapeutic genes as well as their carriers. Macromolecules are internalized into cells by a variety of mechanisms, and their intracellular fate is usually linked to the entry mechanism. Therefore, the successful design of a nonviral gene ...
3. Venault, A., Y. C. Huang, J. W. Lo, Chung-Jung Chou, A. Chinnathambi, A. Higuchi, W. S. Chen, W. Y. Chen and Y. Chang (2017). Tunable PEGylation of Branch-type PEI/DNA Polyplexes with a Compromise of Low Cytotoxicity and High Transgene Expression: in vitro and in vivo Gene Delivery.JOURNAL OF MATERIALS CHEMISTRY B 5(24): 4732-4744 ...
A number of neurodegenerative disorders, such as Parkinsons or Alzheimer Disease, may potentially be treated by gene therapy, i.e. the delivery of therapeutic genes to neurons. Currently, the most common carrier molecules to deliver the therapeutic gene to the patients target cells are viruses that have been genetically altered to carry normal human DNA. Overall gene delivery efficiency is typically low for nonviral vectors. New research undertaken at The Johns Hopkins University offers a systematic approach to understanding the gene delivery process in neurons and explores the intracellular barriers to nonviral gene delivery and possible ways to improve their effectiveness.
Modification of cellular functions by overexpression of genes is increasingly practised for research of signalling pathways, but restricted by limitations of low efficiency. We investigated whether the novel technique of magnetofection (MF) could enhance gene transfer to cultured primary endothelial cells. MF of human umbilical vein endothelial cells (HUVEC) increased transfection efficiency of a luciferase reporter gene up to 360-fold compared to various conventional transfection systems. In contrast, there was only an up to 1.6-fold increase in toxicity caused by MF suggesting that the advantages of MF outbalanced the increase in toxicity. MF efficiently increased transfection efficiency using several commercially available cationic lipid transfection reagents and polyethyleneimine (PEI). Using PEI, even confluent HUVEC could be efficiently transfected to express luciferase activity. Using a green fluorescent protein vector maximum percentages of transfected cells amounted up to 38.7% while ...
Polyethyleneimine-capped silver nanoclusters for microRNA oligonucleotide delivery and bacterial inhibition Chunyuan Du,1,* Haibo Yan,2,* Jichao Liang,1 Ailing Luo,1 Lingqian Wang,1 Jing Zhu,1 Huayu Xiong,3 Yong Chen1 1Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan, 2Department of Internal Medicine, Shandong Medical College, Linyi, 3Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan, China *These authors contributed equally to this work Abstract: Efficient and safe nonviral gene delivery systems are a prerequisite for the clinical application of therapeutic genes. In this paper, polyethyleneimine-capped silver nanoclusters (PEI-AgNCs) were prepared for the purpose of microRNA (miRNA) delivery. The resultant PEI-AgNCs were characterized by a photoluminescence assay
To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have focused on two transfection vehicles: polyethylenimine (PEI) and calcium phosphate (CaPi). Both have been shown to result in high transfection efficiencies at scales beyond 10 L. Unfortunately, both approaches yield higher levels of recombinant protein (r-protein) in the presence of serum than in its absence. Since serum is a major cost factor and an obstacle to protein purification, our goal was to develop a large-scale TGE process for Chinese hamster ovary (CHO) cells in the absence of serum. CHO-DG44 cells were cultivated and transfected in a chemically defined medium using linear 25 kDa PEI as a transfection vehicle. Parameters that were optimized included the DNA amount, the DNA-to-PEI ratio, the timing and solution conditions for complex formation, the transfection medium, and the cell density at the time of transfection. The highest levels of r-protein expression were observed when cultures ...
Objective: Regulated promoter system consented to tightly controlled gene expression is desirable for safe and efficacious overexpression of therapeutic transgenes. Combined with skeletal myoblast (SkMs) transplantation, we report the efficacy of hypoxia-regulated VEGF gene delivery for myocardial repair during acute phase cardiomyopathy.. Methods and Results. A hypoxia-regulated VEGF plasmid (pHRE-VEGF) was developed by inserting 5 copies of hypoxia response elements (HRE). At optimal transfection conditions, flowcytometry revealed that ~30% SkMs were transfected for the gene overexpression using polyethyleneimine nanoparticles. Peak VEGF expression was significantly higher in pHRE-VEGF transfected SkMs (VEGFSkMs) grown under hypoxia (151.34±8.59 ng/ml) as compared with normoxia (16.92±2.74 ng/ml). The efficacy of our hypoxia-regulated gene expression system was assessed in a rabbit model of ischemic cardiomyopathy, developed by permanent coronary artery ligation. The animals were grouped ...
The sulphate activation and tyrosyl-protein sulphotransferase systems in normal 3Y1 rat embryo fibroblasts and the same cells transformed by Schmidt Ruppin subgroup-A-Rous sarcoma virus (SRA-3Y1) were examined. Employing metabolic [35S]sulphate-labelling followed by PEI (polyethyleneimine)-cellulose thin-layer chromatography of the labelled cell lysates, it was found that the steady-state level of active sulphate, adenosine 3′-phosphate 5′-phosphosulphate, was drastically lower in SRA-3Y1 cells compared with their normal counterparts. When the sulphate activating enzymes were tested, it appeared that the activities in 3Y1 homogenates were 2-2.5 times greater than those in SRA-3Y1 homogenates. An endogenous sulphation assay for tyrosyl-protein sulphotransferase revealed that activities in 3Y1 and SRA-3Y1 homogenates were comparable. Nearly identical patterns were observed with both sets of cells when [35S]sulphated proteins generated in the endogenous assay were separated by two-dimensional ...
Media for chromatographic applications, wherein the media is a membrane having a surface coated with a polymer such as a polyethyleneimine. The immobilized polymer coating is modified with a charge-modifying agent to impart quaternary ammonium functionality to the media. The media is well suited for chromatographic purification of virus.
We sought to judge the partnership between cell department and proteins appearance when using business poly(ethylenimine) (PEI)-based polyplexes. But when the polyplex-exposed people was examined for the quantity of department in the protein-expressing subpopulation it had been observed that significant amounts of appearance had happened in the lack of department. Certainly in HeLa S3 cells […]. ...
* found in: Convoy™ Transfection Reagent, Penetratin 1 Peptide, GeneTran™ III Tranfection Reagent, ConvoyTM is new generation cationic polymer gene..
Lipid Polycation DNA (LPD) is Targeted Genetics newest synthetic gene delivery system. Targeted Genetics, in collaboration with Dr L Huang at the University of
Sigma-Aldrich offers abstracts and full-text articles by [Kai Ewert, Ayesha Ahmad, Heather M Evans, Hans-Werner Schmidt, Cyrus R Safinya].
Meet our team booth #A3 during the Cell Therapy Manufacturing Congress in Kyoto (Japan), February 27th - March 2nd, 2018. Our presentation: Optimized PEI-mediated production of clinical grade viral vectors, PEIpro® and PEIpro®-HQ
Page contains details about QD-encapsulated heparinized nanogels/pGFP . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Huang, C. L., Chang, H. W., Chang, J. B., Chen, J-H., Lin, J-D., Wu, C. Z., Pei, D., Hung, Y. J., Lee, C. H., Chen, Y. L. & Hsieh, C. H., 八月 1 2016, 於 : QJM. 109, 8, p. 515-522 8 p., hcv204.. 研究成果: 雜誌貢獻 › 文章 ...
加州克莱尔蒙特--中国的"太子党",那些在教育、就业和商务方面享尽特权的人,正前所未有地经受着外界审视的目光。毛的同志及所谓"不朽"革命者的儿子薄熙来,因为腐败及滥用权力的罪名在不久前被判处无期徒刑。. 国外的太子党们也同样感到了压力。不久前,美国证券交易委员会宣布正在调查摩根大通在香港聘用、并且显然提供了丰厚承销业务的太子党。. 虽然最近的丑闻才让中国太子党进入到媒体耀眼的聚光灯下,但他们一直是西方企业炙手可热的目标。这些企业希望利用其关系获得数十亿美元的交易业务。进行过此类雇佣的金融机构名单与投资银行界名人录如出一辙。 ...
Cíl: Zjistit, které z měřicích nástrojů byly využity k hodnocení kvality života (KŽ) u nemocných s chronickou pankreatitidou (CHP); dohledat případně existující specifický nástroj, který byl validován a standardizován přímo pro CHP; sumarizovat hlavní
结果:与对照组相比,实验组应用激素合并低钙饮食8周骨密度显著性降低(P<0.05),12周进一步降低(P<0.01);实验中两组血钙无显著性差别(P>0.05),实验组4周血磷较对照组降低(P<0.05),8周及12周血磷进一步降低(P<0.01),实验4、8、12周实验组碱性磷酸酶均显著性升高(P<0.01);实验组第12周骨小梁面积百分数(%Tb.Ar)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)较对照组显著降低(P<0.01),骨小梁分离度(Tb.Sp)较对照组显著增高(P<0.01);实验第12周实验组腰椎压缩极限载荷、极限强度和压缩模量均明显低于对照组(P<0.01),股骨三点弯曲极限载荷实验组低于对照组(P<0.05)。 ...
article{8659977, abstract = {Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which is particularly promising in applications for both adherent and suspension cells. In this work, we explored surface modifications of graphene quantum dots (GQD) and reduced graphene oxide (rGO) with polyethylene glycol (PEG) and polyethyleneimine (PEI) to enhance colloidal stability while retaining photoporation functionality. After photoporation with FITC-dextran 10 kDa (FD10), the percentage of positive HeLa cells (81% for GQD-PEG, 74% for rGO-PEG and 90% for rGO-PEI) increased approximately two-fold compared to the bare nanomaterials. While for Jurkat suspension cells, the photoporation efficiency with polymer-modified graphene-based nanomaterial reached as high as 80%. Cell viability was ,80% in all these cases. In addition, polymer functionalization proved to be beneficial for the delivery of larger ...
0361] Cell Line Specific Transfection Reagents/Kits include, e.g., GenJet® (Ver. II) DNA In Vitro Transfection Reagent for 3LL Cell, TransIT®-3T3 Transfection Kit, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for NIH3T3 Cell, Human B Cell Nucleofector® Kit, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for B16-F10 Cells, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for BHK-21 Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for C6 Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for C6 Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for Ca Ski Cell, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for Caco-2 Cell, DG44 Transfection Kit, TransIT®-CHO Transfection Kit, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for CHO Cell, TransIT®-COS Transfection Kit, TransPass® COS Transfection Reagent, GenJet® (Ver. II) DNA In Vitro Transfection Reagent for COS Cell, Targefect-COS, GenJet® (Ver. II) DNA In Vitro Transfection Reagent ...
Abstract : Non-viral gene carriers for safe and efficient gene transfection have become of particular interest among researchers of different disciplines ranging from physical chemistry to biotechnology. Recently polymeric vectors have been extensively studied as potentially new gene transfer agents. Until now most of the research efforts were made to optimize the gene-to-polymer weight ratio of polyplexes for safe and efficient gene transfection. In this work, we report on the development of novel poly(allylamine) derivatives with different balance of the primary, secondary, tertiary, and quaternary amino groups. All derivatives were able to complex pDNA into polyplexes at low gene-to-polymer weight ratios i.e., 1:1 or 1:2. Moreover, the examined polyplexes were less cytotoxic and showed better transfection efficiency when compared to linear poly(ethyleneimine). These results indicate that the presence of quaternary ammonium groups is important in the formation of stable polyplexes. Polymers ...
Purpose. Crosslinked, degradable derivatives of low-molecular-weight polyethylenimine (PEI) are relatively efficient and non-cytotoxic gene delivery agents. To further investigate these promising materials, a new synthetic approach was developed using a poly(4-vinylpyridine)-supported Fe(III) catalyst (PVP(Fe(III))) that provides more facile synthesis and enhanced control of polymer molecular weight.. Methods. Biodegradable polymers (D.PEI) comprising 800-Da PEI crosslinked with 1,6-hexanediol diacrylate and exhibiting molecular weights of 1.2, 6.2, and 48 kDa were synthesized utilizing the PVP(Fe(III)) catalyst. D.PEI/DNA polyplexes were characterized using gel retardation, ethidium bromide exclusion, heparan sulfate displacement, and dynamic light scattering. In vitro transfection, cellular uptake, and cytotoxicity of the polyplexes were tested in human cervical cancer cells (HeLa) and human breast cancer cells (MDA-MB-231).. Results. D.PEIs tightly complexed plasmid DNA and formed 320- to ...
TY - JOUR. T1 - Cationic surface modification of PLG nanoparticles offers sustained gene delivery to pulmonary epithelial cells. AU - Baoum, Abdulgader. AU - Dhillon, Navneet. AU - Buch, Shilpa J. AU - Berkland, Cory. PY - 2010/1/1. Y1 - 2010/1/1. N2 - Biodegradable polymeric nanoparticles are currently being explored as a nonviral gene delivery system; however, many obstacles impede the translation of these nanomaterials. For example, nanoparticles delivered systemically are inherently prone to adsorbing serum proteins and agglomerating as a result of their large surface/volume ratio. What is desired is a simple procedure to prepare nanoparticles that may be delivered locally and exhibit minimal toxicity while improving entry into cells for effectively delivering DNA. The objective of this study was to optimize the formulation of poly(D,L-lactide-co-glycolide) (PLG) nanoparticles for gene delivery performance to a model of the pulmonary epithelium. Using a simple solvent diffusion technique, ...
The treatment of PVT-WGA SPA beads with positively charged polyethyleneimine (PEI) blocks potential non-specific binding sites on the SPA bead surface. There are two SPA bead types available with PEI treatment. The PVT-WGA-PEI type A SPA beads are treated with PEI prior to the coupling of WGA to the PVT SPA bead. The PVT-WGA PEI type B SPA beads are treated with PEI after the WGA coupling stage. The PVT-WGA-PEI type A and type B SPA beads exhibit different characteristics with regard to the non-specific binding of radiolabelled ligand directly to the SPA bead. Therefore, both bead types should be evaluated when deciding which SPA bead to use and both are included in the Select-a-Bead Kit. The binding capacity of both bead types for cell membrane protein remains 10-30 μg membrane protein per milligram of SPA bead.. SPA Scintillation beads are microspheres containing scintillant which emit light in the blue region of the visible spectrum. As a result, these beads are ideally suited to use with ...
SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents... GenJet In Vitro DNA Transfection Reagent for Rin Related Cells [SL100489-RIN] - Description GenJet DNA In Vitro Transfection Reagent for Rin is pre-optimized znd conditioned for transfecting Rin and related cells including Rin-14B, Rin-m5F, Rin-m and Rin-5F. Refer to the following optimal transfection conditions for maximal transfection efficiency on Rin related cells. GenJet reagent, 1.0 ml, is sufficient for 300 to
All-atom molecular dynamics simulations can provide insight into the properties of polymeric gene-delivery carriers by elucidating their interactions and detailed binding patterns with nucleic acids. However, to explore nanoparticle formation through complexation of these polymers and nucleic acids and study their behavior at experimentally relevant time and length scales, a reliable coarse-grained model is needed. Here, we systematically develop such a model for the complexation of small interfering RNA (siRNA) and grafted polyethyleneimine copolymers, a promising candidate for siRNA delivery. We compare the predictions of this model with all-atom simulations and demonstrate that it is capable of reproducing detailed binding patterns, charge characteristics, and water release kinetics. Since the coarse-grained model accelerates the simulations by one to two orders of magnitude, it will make it possible to quantitatively investigate nanoparticle formation involving multiple siRNA molecules and ...
The choice of a transfection reagent often depends more upon the particular cell line than the substance being delivered into the cells. We have available four different lipid-based transfection reagents that have been specially formulated for delivery of siRNAs into cells. These DharmaFECT reagents are very mild yet have very high transfection rates into a wide variety of cell lines. You can find a list of cells weve tested with DharmaFECT here: http: //dharmacon. horizondiscovery. com/transfection/dharmafect-cell-type-guide/Another recommendation is to use a reagent that has been previously used in the cells of interest. Further optimization for siRNA delivery may be necessary. If no established protocol for the cells is available, a PubMed or HighWire search may help identify if a published protocol for the specific cell line or a similar cell line has been reported. In all instances, we recommend following the protocol provided by the manufacturer of the transfection reagent ...
TY - JOUR. T1 - Nonviral gene delivery to human breast cancer cells by targeted Ad5 penton proteins. AU - Medina-Kauwe, L. K.. AU - Maguire, M.. AU - Kasahara, N.. AU - Kedes, L.. PY - 2001/12/1. Y1 - 2001/12/1. N2 - The capsid proteins of adenovirus serotype 5 (Ad5) are key to the virus highly efficient cell binding and entry mechanism. In particular, the penton base plays a significant role in both viral internalization and endosome penetration. We have produced an adenovirus penton fusion protein (HerPBK10) containing moieties for DNA transport and targeted delivery to breast cancer cells. HerPBK10 binds DNA through a polylysine appendage, while the EGF-like domain of the heregulin-α1 isoform is used as the targeting ligand. This ligand binds with high affinity to HER2/3 or HER2/4 heterodimers, which are overexpressed on certain aggressive breast cancers. In addition, this ligand is rapidly internalized after binding, thus adding to the utility of heregulin for targeting. HerPBK10 binds ...
The efficiency of non-viral gene delivery is dependent on 4 factors: DNA protection, DNA delivery across membranes, endosomal DNA release, and nuclear uptake. Here we report on the development of a new delivery technology based on the in situ layer by layer synthesis of DNA nanoplexes within hollow yeast cell wall particles (YCWP). YCWP provides protection and facilitates oral and systemic receptor-targeted delivery of DNA payloads to phagocytic cells and tissues containing these cells. Using DNA encoding green fluorescent protein (GFP) as a model system we have evaluated nanomaterials capable of overcoming these membrane hurdles. Outer nanomaterial layers have been identified that protect the DNA payload and enhance endosomal DNA release. Low levels of polyethylenimine, cationic surfactants and cationic peptides have proven effective. Inner nanomoaterial layers have been synthesized with components designed to promote DNA decomplexation and nuclear uptake to maximize transfection efficiency. Cationic
Disclosed is a diagnostic test device which comprises a fluid permeable structural support member having on one side thereof a layer of a macroporous membrane comprising polyethyleneimine impregnated with iodate ion and on the other side of the support member having a layer of a microporous membrane having dispersed therein a chromogenic indicator capable of providing a colored response upon being oxidized. The intensity of the colored response is less subject to ascorbate interference due to the activity of the iodate ion.
G-FECT™ DNA Transfection reagents are a series of powerful DNA transfection reagents with high efficiency DNA delivery and low cell toxicity into mammalian cells. G-FECT™ can be used for plasmid transfection, genome editing, virus production and more.
Lipofectamine 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene exp
GeneJuice® Transfection Reagent Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammallian cells. - Find MSDS or SDS, a COA, data sheets and more information.
Surface treatment compositions comprising certain cationic polymer(s), anionic surfactant, one or more shielding salts and hydrophobic association disruptor. The surface treatment compositions comprise at least about 6 % by weight of cationic polymer, at least about 6% by weight anionic surfactant, and at least about 4 % by weight of the shielding salt. The weight ratio of anionic surfactant to cationic polymer is between about 0.5:1 and about 4:1. The composition may also have a weight ratio of shielding salt to cationic polymer of between about 0.3:1 and about 3:1.
InvivoGen provides LyoVec, a lyophilized cationic lipid-based transfection reagent made of phosphonolipid DTCPTA coupled with DiPPE, a neutral lipid that helps destabilizing membrane bilayers, therefore increasing the in vitro transfection efficiency of LyoVec.
* This reagent has two solutions: Solution A - 0.5 ml, Solution B - 1.0 ml The TransPass™ R2 Transfection Reagent is a two component non-liposomal reagent combination developed specifically for efficient transfection of siRNA in a variety of mammalian cell lines that include “difficult to transfect cells such as primary cells