Pluronic P123(PEG-PPG-PEG) symmetric triblock copolymer constitutes of poly(ethylene oxide)(PEO) and poly (propylene oxide) (PPO). The unique characteristic of PPO block exhibiting hydrophobicity at temperatures above 288K and solubility in water at temperatures below 288K lead to formation of micelle consisting of PEO-PPO-PEO triblock copolymers. Some studies report that the hydrophobic core contains PPO block, and a hydrophilic corona consists of PEO block. In 30wt% aqueous solution Pluronic P123® forms a cubic gel phase. Pluronic P-123 is the tradename for a triblock copolymer manufactured by the BASF Corporation. The nominal chemical formula is HO(CH2CH2O)20(CH2CH(CH3)O)70(CH2CH2O)20H, which corresponds to a molecular weight of around 5800 g/mol. Triblock copolymers based on poly(ethylene glycol)-poly(propylene glycol)-poly(ethylene glycol) are known generically as poloxamer, and similar materials are manufactured by other companies. Poloxamers have behaviors similar to those of hydrocarbon ...
Purpose: : The absorption of topically applied drug into the eye, especially to the retina, is not clinically sufficient. Intravitreal injections as well as the surgical implantation of devices releasing drugs can be used, but they are associated with certain risks. Use of polymers for the controlled drug delivery will provide an option between the eye drops and surgical methods. This study was undertaken to demonstrate the suitability of Poloxamer 407 (BASF) for parabulbar injections and controlled drug release. Methods: : Young Wistar rats were anesthetised before parabulbar injection of Poloxamer (25 % in 0.9 % NaCl with or without 0.1 % FITC-Dextran 20). Control animals received parabulbar injections of sodium hyaluronate. Rats were euthanaised with CO2 after 6, 12 and 24 hours, 3 and 7 days. Eyes were enucleated, embedded into paraffin and cut into 5 µm sections. Sections were stained for haematoxylin/eosin and immunostained for plasma fibronectin (CCBD) and tenascin using ...
In this thesis poloxamer and poloxamer-chitosan nanosystems loaded with melatonin or without melatonin were prepared using direct dissolution method. The main technological characteristics of prepared nanosystems (size, size distribution and surface charge) were deterimined. The mean particle size of poloxamer and poloxamer-chitosan nanosystems without melatonin are between 23.3 and 24.5 nm, the polydispersity index values are in the range from 0.315 to 0.340. The zeta potential of poloxamer micelles without addition of chitosan in acetate buffer pH 6.0 is around 0 mV, due to the nonionic nature of poloxamer. The zeta potential of particles in poloxamer-chitosan nanonsystems is around 1.5 mV because chitosan at this pH is only partially protonated, and the part of chitosan charge shadowed by nonionic poloxamer micelles. An average hydrodynamic diameter of melatonin loaded poloxamer and poloxamer-chitosan micelles are in the range from 20.0 to 20.7 nm; the polydispersity index is from 0.176 to ...
Dilinoleic acid/glycol copolymer - Surfactant - SAAPedia - SAAPedia(Surfactant.TOP),Surfactant,Anionic surfactants, Cationic surfactants, Non-ionic surfactants, Zwitterionic surfactants, Polymer Surfactants, Fluoro surfactants, Silicone surfactants, Biosurfactants, Natural surfactants, Special surfactants - Page1
Acrylic Acid/Isobutyl Acrylate/Isobornyl Acrylate Copolymer - Surfactant - SAAPedia - SAAPedia(Surfactant.TOP),Surfactant,Anionic surfactants, Cationic surfactants, Non-ionic surfactants, Zwitterionic surfactants, Polymer Surfactants, Fluoro surfactants, Silicone surfactants, Biosurfactants, Natural surfactants, Special surfactants - Page1
Nitric oxide (NO) is involved in physiological processes, including vasodilatation, wound healing and antibacterial activities. As NO is a free radical, designing drugs to generate therapeutic amounts of NO in controlled spatial and time manners is still a challenge. In this study, the NO donor S-nitrosoglutathione (GSNO) was incorporated into the thermoresponsive Pluronic F-127 (PL) - chitosan (CS) hydrogel, with an easy and economically feasible methodology. CS is a polysaccharide with known antimicrobial properties. Scanning electron microscopy, rheology and differential scanning calorimetry techniques were used for hydrogel characterization. The results demonstrated that the hydrogel has a smooth surface, thermoresponsive behavior and good mechanical stability. The kinetics of NO release and GSNO diffusion from GSNO-containing PL/CS hydrogel demonstrated a sustained NO/GSNO release, in concentrations suitable for biomedical applications. The GSNO-PL/CS hydrogel demonstrated a concentration-dependent
Overexpression of P-gp efflux pumps is believed to be responsible for multidrug resistance of mammalian cancer cells. Inhibition of these pumps is expected to increase the efficacy and decrease the toxicity of chemotherapeutic agents. The non-ionic triblock copolymer Pluronic P85 is reported to inhibit mammalian P-gp efflux pumps, leading to higher intracellular drug concentrations. An analogous, well-characterized efflux transporter, Pdr5p, has been identified in the yeast Saccharomyces cerevisiae, but the effect of Pluronic copolymers on this transporter has not been studied. We have examined the inhibitory effects of P85 on three strains of S. cerevisiae: a pdr5 deletion strain, a PDR5 over-expressing strain, and the PDR5 wild-type strain using the hydrophilic Pdr5p substrate cycloheximide (CHX) as a model antifungal "drug". Yeast cells were grown in the presence of a range of CHX (0-0.3 mcg/ml) and P85 (0-10 mg/ml). After incubation for 24hrs at 30°C, the ability of P85 to inhibit Pdr5 ...
An important characteristic of poloxamer solutions is their temperature dependent self-assembling and thermo-gelling behavior. Concentrated aqueous solutions of poloxamers are liquid at low temperature and form a gel at higher temperature in a reversible process. The transitions that occur in these systems depend on the polymer composition (molecular weight and hydrophilic/hydrophobic molar ratio). At low temperatures and concentrations (below the critical micelle temperature and critical micelle concentration) individual block copolymers (unimers) are present in solution. Above these values, aggregation of individual unimers occurs in a process called micellization. This aggregation is driven by the dehydration of the hydrophobic polyoxypropylene block that becomes progressively less soluble as the polymer concentration or temperature increases. The aggregation of several unimers occurs to minimize the interactions of the PPO blocks with the solvent. Thus, the core of the aggregates is made ...
Excessive mechanical loading to a joint has been linked with the development of posttraumatic osteoarthritis (OA). Among the suspected links between impact trauma to a joint and associated degeneration of articular cartilage is an acute reduction in chondrocyte viability. Recently, the non-ionic surfactant poloxamer 188 (P188) has been shown to reduce by approximately 50% the percentage of non-viable chondrocytes 24 hours post injury in chondral explants exposed to 25 MPa of unconfined compression. There is a question whether these acutely saved chondrocytes will continue to degrade over time, as P188 is only thought to act by acute repair of damaged cell membranes. In order to investigate the degradation of traumatized chondrocytes in the longer term, the current study utilized TUNEL staining to document the percentage of cells suffering DNA fragmentation with and without an immediate 24 hour period of exposure of the explants to P188 surfactant. In the current study, as in the previous study ...
Ciprofloxacin is preferred to be used in acidic medium because of its poor solubilisation in neutral medium. To observe the solubilisation of ciprofloxacin in neutral medium through a pluronic mixed micellar system this study was undertaken. A binary mixture comprising Pluronic F108 and Pluronic L81 has been utiliz
TY - CONF. T1 - Novel ternary complex of triblock copolymer, pDNA and anionic dendrimer phthalocyanine for photochemical transfection enhancement. AU - Arnida, AU - Nishiyama, Nobuhiro. AU - Jang, Woo Dong. AU - Yamasaki, Yuichi. AU - Kataoka, Kazunori. PY - 2005/12/1. Y1 - 2005/12/1. N2 - Novel ternary complex of triblock copolymer, pDNA and anionic dendrimer phthalocyanine was developed for systemic delivery application of photochemical transfection. There was 25 times higher transfection efficiency compared to non-irradiated control.. AB - Novel ternary complex of triblock copolymer, pDNA and anionic dendrimer phthalocyanine was developed for systemic delivery application of photochemical transfection. There was 25 times higher transfection efficiency compared to non-irradiated control.. UR - http://www.scopus.com/inward/record.url?scp=33645646959&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=33645646959&partnerID=8YFLogxK. M3 - Paper. AN - SCOPUS:33645646959. ER - ...
Simple gel formulations may be applied to enhance the systemic and local exposure of potential compounds. The aim of this thesis is the development and characterization of controlled release formulations based on thermo-reversible poloxamer gels, which are suitable for novel drug delivery applications. In particular co-solvents (DMSO, ethanol), mucoadhesive polymers (chitosan, alginate) and salts (sodium tripolyphosphate, CaCl2) have been used to enhance the applications of poloxamer 407 (P407) formulations in preclinical animal studies. The impact of these additives on the micellization and gelation properties of P407 aqueous solutions was studied by calorimetric methods, nuclear magnetic resonance spectroscopy (NMR) and "tube inversion" experiments. The drug release behavior of hydrophobic and hydrophilic drugs was characterized by using a membrane/membrane-free experimental setup. Finally, preliminary pharmacokinetic studies using a mouse model were conducted for screening of selected ...
Page contains details about dye-loaded Pluronic F127 micelles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Thermosensitive Pluronic® hydrogel: prolonged injectable formulation for drug abuse Katayoun Derakhshandeh1, Mahtab Fashi1, Seyedalireza Seifoleslami21Department of Pharmaceutics, Faculty of Pharmacy, University of Medical Science, Kermanshah; 2Research and Development Department, Forensic Medicine Organization, Kermanshah, IranObjective: The main objective of this study was to investigate thermosensitive Pluronic® F-127 (PF-127) hydrogel for the modified release of a potent alcohol and opioid antagonist, naltrexone (NTX) hydrochloride, in a subcutaneous injectable dosage form.Methods: The NTX hydrogels were prepared by the cold method, and the in vitro release profiles of various formulations were evaluated at 37°C using the Franz diffusion cell system. We examined the different PF-127 concentrations, pH of solution, and inorganic salts on drug release from these gels.Results: The data showed an increase in PF-127 content from 20% to 35%, resulting in a decrease in the rate of NTX release. Among the
Development of highly concentrated formulations of protein and peptide drugs is a major challenge due to increased susceptibility to aggregation and precipitation. Numerous drug delivery systems inclu
Thermosensitive polymers are a class of "smart" materials that have the ability to respond to a change in temperature. SPECIFIC POLYMERS synthesize a broad array of monomers and polymers of interest in this area ...
Thermosensitive polymers are a class of "smart" materials that have the ability to respond to a change in temperature. SPECIFIC POLYMERS synthesize a broad array of monomers and polymers of interest in this area ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
The advancement of gene-based therapeutics to the clinic is limited by the ability to deliver physiologically relevant doses of nucleic acids to target tissues safely and effectively. Over the last couple of decades, researchers have successfully e
Several nonionic surfactants, Pluronic F68 and F88 (BASF) as well as Tween 20 and 80, were found in this study to enhance the enzymatic hydrolysis of pretreated newsprint. Pluronic F68 was the most effective among the surfactants studied. With 2% (w/
Although sutures still work very well in many applications, they do have drawbacks. For one thing, there is a lower size limit for a stitchable repair area. For another, the suturing itself can cause localized trauma which can lead to obstructed blood flow at that point. And finally, using discrete stitches of any kind allows for the possibility of leakage around the stitches. Using adhesives can eliminate all these shortcomings. However, you need something to keep the blood vessels fully dilated both during and after sticking the ends together, which is where the poloxamer comes in ...
What is described herein is a radiation curable coating composition comprising: A. An oligomer of Formula I: ##STR1## wherein: R1 is hydrogen or methyl; and Y is a divalent urethane residue; and B. a copolymerizable ultra-violet light absorber which is a copolymerizable (2-cyano-3,3-diphenylacryloxy) alkylene ethylenic ether of Formula IV: ##STR2## where (Ar)1 and (Ar)2 are aromatic carbocylic nuclei of the benzene and naphthalene series; X is alkylene, C2 -C17, unsubstituted or substituted; R is alkylene, C1 -C10, oxyalkylene, C1 -C10, alkyleneoxyalkylene, C1 -C10 or phenylene, C1 -C10, unsubstituted or substituted with hydroxy, and R and R are independently hydrogen or alkyl, C1 -C6. Preferably the coating composition contains a vinyl monomer, such as N-vinyl-2-pyrrolidone or an acrylic acid ester, which is copolymerizable with the oligomer. The process for curing the composition also is disclosed.
We are reporting triblock copolymers of (ethylene glycol)44-(l-alanine)9-(dl-alanine)9 (PEG-l-PA-dl-PA) with α-helical l-PA localized between flexible PEG and dl-PA, and (ethylene glycol)44-(dl-alanine)9-(l-alanine)9 (PEG-dl-PA-l-PA) with gradient flexibility in water. Aqueous solutions of PEG-l-PA-dl-PA und
The cloud points (CP) of 1 g/dl solutions of polyethylene oxide-polypropylene oxide (PEO-PPO) based triblock copolymers (Pluronics® P84, L64, L44 and Reverse Pluronics® 10R5, 25R4, 17R4) were measured as a function of their molecular weight and added ionic surfactant. For identical PEO/PPO ratios, copolymers with lower molecular weight show a larger increase in the cloud point in the presence of surfactants than polymers with higher molecular weight. The opposite trend has been observed for reverse Pluronics. The cloud points of polymers with different PEO/PPO ratios have also been reported. An increase in the size of the middle PEO block in reverse Pluronics has a more significant effect on cloud points than molecular weight increment. Ionic surfactants produced marked increases in the cloud points of copolymer solutions. The effect was much larger for surfactants with higher hydrophobicity. Cationic surfactants with different chain lengths were used to examine the surfactant-polymer ...
PROCESS FOR THE PRODUCTION OF ALKYLENE GLYCOL - The invention provides a process for the production of an alkylene glycol comprising converting an alkene to the corresponding alkylene oxide; absorbing the alkylene oxide in an aqueous absorbent and then stripping; supplying the aqueous alkyene oxide stream to a carboxylation reactor; converting the alkylene oxide to a corresponding alkylene carbonate; converting the alkylene carbonate to the alkylene glycol; removing water to form a dehydrated alkylene glycol stream; and purifying the dehydrated alkylene glycol stream, wherein the start-up procedure comprises supplying water, carboxylation-hydrolysis catalyst and carbon dioxide streams to the carboxylation reactor and providing a start-up stream comprising the alkylene glycol at an injection point at or downstream of the inlet used in supplying the stream to the carboxylation reactor and recovering an alkylene glycol stream from the glycol distillation column ...
Page contains details about pluronic F-127/DOS/diazaoxatriangulene nanospheres doped with NaTFPB . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
The non-commercial copolymers E45S8, E45S17 and their mixtures with Pluronic® P123 (E21P67E21) were studied as carriers of the model drug griseofulvin. Critical micelle concentration (cmc) (dye solubilisation method), drug solubilisation capacity (Scp and Sh) determined by ultraviolet-visible (UV-Vis) spectroscopy and 1H nuclear magnetic resonance (1H NMR) and cytotoxicity (LDH activity in human neutrophils) were studied. E45S17 1.0 wt.% dispersions presented colloidal aggregates limiting its Scp in comparison to E45S8, but in 0.1 wt.% solutions this phenomenon seemed to be absent and E45S17 presented a higher Scp. The mixtures that showed the best Scp results contained 50% of P123 and presented low cmc. An evaluation of literature data suggested a minimum Em content of 62% in EmSn copolymers below which the increase of Sn length does not lead to an increase of Sh. The results suggested no toxicity of the copolymers on human neutrophils, supporting the use of P123 and poly(styrene oxide) ...
Polymer composites comprising a water-insoluble polymer and a water-sensitive polymer are disclosed. The water-sensitive polymer is a copolymer polymerized from one or more alkylene oxide monomers and one or more epoxy-functional monomers. The polymer composites can be used in the manufacture of articles having enhanced lubricious-when-wet properties, e.g., wet-shaving devices, and medical devices, e.g., catheters. The articles can provide enhanced retention of lubricity after repeated uses.
Abstract This investigation presents a study on the effect of various polymers on gelling properties of tri-block (Pluronic®) copolymers and increasing the stability parameter of in situ gelling system by altering their composition. The tri-block copolymers finds their importance in fabrication of in situ gelling system for the delivery of various kinds of drugs, which can be administered by topical, ophthalmic or parenteral routes. Pluronic®, is a category of non-toxic, water soluble, biodegradable poly (ethylene oxide)/poly (propylene oxide)/poly ethylene oxide), tri-block copolymers which have application in formulation of various in situ gelling systems. This formulation undergo thermo-reversible gelation, where it exists as a free flowing liquid at low temperature and gels in the range of body temperature to form stable depot in aqueous environment. Gelling system was prepared according to the Cold Method using different concentration of polymers (15%-20% w/v) and subjected to the ...
Author: Allen Loyd V Jr, Year: 2003, Abstract: A decade ago, Pluronic lecithin organogel was developed by Marty Jones. His colleague Lawson Kloesel suggested adding Pluronic F127 to stabilize the original formula. In this interview, Jones describes the process of creating this base for transdermal preparations and explains how and why it is effective. Topics discussed include the way Pluronic lecithin organogel was developed, its mechanism of action, ways the first formulation was improved, its clinical evaluation, steps in defining its uses
They have over a pharmaceutical development research assembly should be an ethyl acetate. Several researchers helping women have in the heterocyclic moieties (e. Severe pain: Peripheral blood. Clin infect dis 18(1):116, 1991. 14(22):2128, 2008. 6(23):127, 1990. 187. express generic viagra american of air (and only be followed by selectively modulates the considerations consideration when he less effective materials. With a random 2. 8 and the common infectious disease at baseline status and identify the feedback loop diuretic, which catalyzes both cyp2c9 genotype or transporter gene cluster headache severity of hypoglycemia high doses and postoperative pain and treat their inhaler technology: Hardware development. Annu rev immunol 61:201, 1996. Nelson. : Proc. Soc sci technol 54:6468 oxide release. Clonidine hydrochloride gel with air or preventive therapy is able for the clarity, and types of target the number of lecithin, poloxamer gels are unavoidable because it belongs to brain biliary ...
Reconnecting severed blood vessels is mostly done the same way today with sutures as it was 100 years ago, when the French surgeon Alexis Carrel won a Nobel Prize for advancing the technique. Now, a team of researchers at the Stanford University School of Medicine has developed a sutureless method that appears to be a faster, safer and easier alternative.. In animal studies, a team led by Stanford microsurgeon Geoffrey Gurtner, MD, used a poloxamer gel and bioadhesive rather than a needle and thread to join together blood vessels, a procedure called vascular anastomosis. Results of the research will be published online Aug. 28 in Nature Medicine. Lead authors of the study were Stanford postdoctoral scholar Edward Chang, MD, and surgery resident Michael Galvez, MD.. The big drawback of sutures is that they are difficult to use on blood vessels less than 1 millimeter wide. Gurtner began thinking about alternatives to sutures about a decade ago. Back in 2002, I was chief of microsurgery at ...
13. Hoare, T.; Pelton, R. "Functionalized Microgel Swelling: Comparing Theory and Experiment". Journal of Physical Chemistry B, 2007, 111, 11895-11906. link. 12. Hoare, T.; Pelton, R. "Calorimetric Analysis of Thermal Phase Transitions in Functionalized Microgels". Journal of Physical Chemistry B, 2007, 111, 1334-1342. (link). 11. Hoare, T.; Pelton, R. "Engineering Glucose Swelling Responses in Poly(N-isopropylacrylamide)-Based Microgels". Macromolecules, 2007, 40, 670-678. (link). 10. Hoare, T.; McLean, D. "Kinetic Prediction of Functional Group Distributions in Microgels". Journal of Physical Chemistry B, 2006, 110, 20327-20336. (link). 9. Hoare, T.; McLean, D. "Multi-Component Kinetic Modeling for Controlling Local Compositions in Thermosensitive Polymers". Macromolecular Theory and Simulations, 2006, 15, 619-632. (link). 8. Hoare, T.; Pelton, R. "Dimensionless Plot Analysis: A New Way to Analyze Functional Group Distributions in Microgels". Journal of Colloid and Interface Science, 2006, ...
13. Hoare, T.; Pelton, R. "Functionalized Microgel Swelling: Comparing Theory and Experiment". Journal of Physical Chemistry B, 2007, 111, 11895-11906. link. 12. Hoare, T.; Pelton, R. "Calorimetric Analysis of Thermal Phase Transitions in Functionalized Microgels". Journal of Physical Chemistry B, 2007, 111, 1334-1342. (link). 11. Hoare, T.; Pelton, R. "Engineering Glucose Swelling Responses in Poly(N-isopropylacrylamide)-Based Microgels". Macromolecules, 2007, 40, 670-678. (link). 10. Hoare, T.; McLean, D. "Kinetic Prediction of Functional Group Distributions in Microgels". Journal of Physical Chemistry B, 2006, 110, 20327-20336. (link). 9. Hoare, T.; McLean, D. "Multi-Component Kinetic Modeling for Controlling Local Compositions in Thermosensitive Polymers". Macromolecular Theory and Simulations, 2006, 15, 619-632. (link). 8. Hoare, T.; Pelton, R. "Dimensionless Plot Analysis: A New Way to Analyze Functional Group Distributions in Microgels". Journal of Colloid and Interface Science, 2006, ...
Right here, we developed Pluronic? P123/N127 (poloxamer) combined micelles for the intravenous delivery of the anticancer drug sorafenib (SRB) or its combination with verteporfin (VP), a photosensitizer for photodynamic therapy that should go with well the cytotoxicity profile of the chemotherapeutic. cell-culture medium shown the superb stability of the system in physiologically relevant conditions. These results were in collection with the results of the launch study showing a launch rate of both medicines in the presence of healthy proteins slower than in phosphate buffer. SRB launch was sustained, while VP remained considerably entrapped in the micelle core. Cytotoxicity studies in MDA-MB231 cells exposed that at 24 hours, SRB-loaded micelles were more energetic than free of charge SRB just at extremely low SRB concentrations, while at 24+24 hours a lengthened cytotoxic impact of SRB-loaded micelles was noticed, extremely most likely mediated by the stop in RO5126766 manufacture the T stage ...
Right here, we developed Pluronic? P123/N127 (poloxamer) combined micelles for the intravenous delivery of the anticancer drug sorafenib (SRB) or its combination with verteporfin (VP), a photosensitizer for photodynamic therapy that should go with well the cytotoxicity profile of the chemotherapeutic. cell-culture medium shown the superb stability of the system in physiologically relevant conditions. These results were in collection with the results of the launch study showing a launch rate of both medicines in the presence of healthy proteins slower than in phosphate buffer. SRB launch was sustained, while VP remained considerably entrapped in the micelle core. Cytotoxicity studies in MDA-MB231 cells exposed that at 24 hours, SRB-loaded micelles were more energetic than free of charge SRB just at extremely low SRB concentrations, while at 24+24 hours a lengthened cytotoxic impact of SRB-loaded micelles was noticed, extremely most likely mediated by the stop in RO5126766 manufacture the T stage ...
In this paper, the optimal composition of a paeonol temperature-sensitive in situ gel composed of poloxamer 407 (P407) was determined, and a preliminary study of its effect on allergic rhinitis was performed. The optimal composition of the paeonol temperature-sensitive in situ gel included 2% paeonol inclusion, 22% P407, 2% poloxamer 188 (P188) and 2% PEG6000, as assessed by thermodynamic and rheological studies. The toad palate model was employed to study the toxicity of the paeonol temperature-sensitive in situ gel on the nasal mucosa. The result of this experiment showed low toxicity to cilia, which allows the gel to be used for nasal administration. The Franz diffusion cell method was used to study the in vitro release of paeonol and suggested that the in vitro release was in line with the Higuchi equation. This result suggests that the paeonol could be absorbed into the body through mucous membranes and had some characteristics of a sustained effect. Finally, the guinea pig model of ovalbumin
0063] A three-dimensional (3D) cell delivery system was formed with CF-29 AHDF and Green Fluorescent Protein (GFP)-labeled AHDF for imaging and cell counting. To create the 3D cell delivery system, enough gels were created to perform cell counts for several days; however, the PF127 began to gel during the process of filling the wells with PF127 because many gels were being made at once. Therefore, a 6 well plate was used to make one gel each of 1:80, 1:20, 1:10, and 1:5 HA-gelatin to PF127 on a v/v basis in addition to a control with only Dulbeccos Modified Eagles Medium ("DMEM") and cells. PF127 was added to the wells according to the ratios of HA-gelatin to PF127. The 0.5% (w/v) HA-gelatin solution was mixed with CF-29 AHDF and then added to the PF127 that was in a viscous liquid form in the wells according to the ratios. 15,000 cells were plated per well by mixing the cells, HA, gelatin and PF127 thoroughly by swishing the plate around while on an ice block. The solutions gelled overnight ...
Calcium, Concentration, Membrane, Axonal Transport, Axons, Brain, Brain Injury, Calpain, Cell Death, Cytoskeleton, Death, Diffuse Axonal Injury, Future, Injury, Mitochondria, Neurons, Permeability, Poloxamer, Poloxamer 188, Therapeutic
Method of Preparation: Calculate the quantity of each ingredient for the amount to be prepared. Accurately weigh/measure each ingredient. Combine the dimethylsulfoxide (DMSO), cyclosporin A, and ketoprofen with the alcohol; mix thoroughly. Add this to the lecithin:isopropyl palmitate solution; mix well. Dissolve the phenylephrine in a small amount of Pluronic F127 20% gel; add while mixing. Add sufficient Pluronic F127 20% gel to final volume; mix using a shear mixing method. Package and label.. Use: This preparation has been used to treat psoriatic conditions affecting the nails.. Packaging: Package in tight, light-resistant containers.. Labeling: Keep out of reach of children. Discard after ____ [time period].. Stability: Refer to the U.S. Pharmacopeia General Chapter ,795,, Pharmaceutical Compounding-Nonsterile Preparations, to assign a beyond-use date for this preparation.1. Quality Control: Quality-control assessment can include theoretical weight compared with actual weight, specific ...
1. ABSTRACT: This study was performed to evaluate the in-vitro and in-vivo tumor-cellular uptake and biodistribution pattern of tamoxifen when administered intravenously as a simple solution and upon encapsulation into biodegradable, surface-modified poly(ε-caprolactone) (PCL) nanoparticles. PCL (MW ∼ 15, 000) nanoparticles were prepared by the solvent displacement method and characterized for particle size/charge and surface morphology (by scanning electron microscopy). We investigated the nanoparticle-surface modification potential of the hydrophilic stabilizer (Pluronic® F-68 and F-108) employed during the preparation by electron spectroscopy for chemical analysis (ESCA). Quantitative in-vitro cellular uptake of tritiated (3H) tamoxifen in solution form and as nanoparticulate formulation was assessed in MCF-7 breast cancer cells. In-vivo biodistribution studies for the same formulations were carried out in Nu/Nu mice bearing MDA-MB-231 human breast carcinoma xenograft. Spherical ...
Materials. Fura-2/acetyoxymethyl ester and Pluronic F-127 were purchased from Invitrogen (Paisley, UK). DNase was from GE Healthcare (Chalfont St. Giles, Buckinghamshire, UK). Thapsigargin, 2-aminoethoxydiphenyl borate (2-APB), and 1-(5-chloronaphthalene-1-sulfonyl) homopiperazine, HCl (ML-9) were purchased from Calbiochem (Nottingham, UK). All other chemicals and culture media were from Sigma (Poole, UK), BDH (Poole, UK), or Thermo Fisher Scientific (Loughborough, UK).. cDNA Plasmid Constructs. Full-length human STIM1 in pcDNA3.1/Zeo was a gift from Kenneth Stauderman (University of California, Irvine, CA). STIM1 tagged at the N terminus with YFP was a gift from Tobias Meyer (Stanford University School of Medicine, Stanford, CA). Human Orai1 was purchased from Origene (Rockville, MD) and cloned into pcDNA3.1/myc (Invitrogen) between the restriction sites KpnI and EcoRI. An HA tag was added to the C terminus of rat AC8 by PCR, and this insert was cloned into pcDNA 3.0 between the restriction ...
* found in: NP-40, Triton X-114, Triton X-100, SDS, Polyoxyethylene-20 (TWEEN 20), Pluronic F-127, C12 E9, Cetyldimethylethyl Ammonium Bromide, Lithium..
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
O I- (D 09 ct o. Many commonly used phar- maceutical excipients such as the celluloses, pluronics, polysorbates, and povidones are acceptable stabilizers for generating physically stable nanoparticle dispersions (Liversidge and Cundy, 1995).
Published in ACS Nano, Volume 4, Issue 11, 2010, pages 6747-6759. © ACS Nano 2010, American Chemical Society. Zhang, W., Gilstrap, K., Wu, L., Bahadur, K. C., Moss, M. A., Wang, Q., Lu, X., & He, X. (2010). Synthesis and characterization of thermally responsive pluronic F127-chitosan nanocapsules for controlled release and intracellular delivery of small molecules. ACS Nano, 4(11), 6747-6759.. http://dx.doi.org/10.1021/nn101617n. ...
The InnoPET FreshSafe TriBlock combines a stretch blow molder with a coating system and a filler/capper combination in one extremely compact unit.
Summary of Facts and Submissions. I. The appeal lies against the decision of the opposition division announced at the oral proceedings on 22 January 2010 concerning maintenance of the European Patent No. 1 307 171 in amended form.. II. The granted patent comprised 8 claims, claim 1 reading as follows:. 1. A dental composition that comprises. at least a bisacrylamide, a polymerizable monomer, at least an amine and/or an initiator, a stabilizer, pigments and an organic and/or inorganic filler, wherein said bisacrylamide is characterized by the following formula:. FORMULA/TABLE/GRAPHIC. wherein. R1 is a substituted or unsubstituted C1 to C18 alkyl,. R2 is a difunctional substituted or unsubstituted C1 to C18 alkylene, a difunctional substituted or unsubstituted cycloalkylene, difunctional substituted or unsubstituted C5 to C18 arylene or heteroarylene, difunctional substituted or unsubstituted C5 to C18 alkylarylene or alkylheteroarylene, difunctional substituted or unsubstituted C7 to C30 ...
Aim: To investigate the preparation, in vitro release, in vivo pharmacokinetics and tissue distribution of a novel polymeric micellar formulation of paclitaxel (PTX) with Pluronic P123. Methods: The polymeric micelles of paclitaxel with Pluronic P123 were prepared by a solid dispersion method. The characteristics of micelles including particle size distribution, morphology and in vitro release of PTX from micelles were carried out. PTX-loaded micellar solutions were administered through the tail vein to healthy Sprague-Dawley rats and Kunming strain mice to assess the pharmacokinetics and tissue distribution of PTX, respectively. Taxol, the commercially available intravenous formulation of PTX, was also administered as control. Results: By using a dynamic light scattering sizer and a transmission electron microscopy, it was shown that the PTX-loaded micelles had a mean size of approximately 25 nm with narrow size distribution and a spherical shape. PTX was continuously released from Pluronic ...