Reeve, J G. and Twentyman, P R., "Ploidy distribution of tumour cells derived from induced and spontaneously arising metastases of a murine radiation-induced sarcoma, rif-1." (1982). Subject Strain Bibliography 1982. 1155 ...
Objective: To investigate underlying genetic events associated with complex DNA ploidy breast carcinomas.. Methods: Screening for chromosome imbalances was carried out using comparative genomic hybridisation (CGH) in 14 frozen samples of tumour from a series of 13 breast cancer patients with multiploid (n = 11) and hypertetraploid (n = 2) tumours. They had previously been analysed by DNA flow cytometry and also assessed immunohistochemically for p53 tissue expression. Ploidy status was determined on frozen samples using the Multicycle software program.. Results: The total number of copy gains (n = 242) was significantly greater than the number of copy losses (n = 51). The mean (SD) number of gains per sample was 17.3 (5.7), and of losses, 3.6 (4.2) (p = 0.0001). Gains of chromosomal regions at 1q (14/14; 100%), 7q (12/14; 85.7%), and 3q (11/14; 78.6%), as well as 1p, 2q, 5p, 8q, and 13q (10/14; 71.4%) were the most frequent aberrations in this series. Losses were most commonly found on 17p ...
Principal Investigator:TAKIHARA Hiroshi, Project Period (FY):1992 - 1993, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Urology
To investigate the colonic adenoma-adenocarcinoma progression sequence, DNA ploidy analysis was performed on hyperplastic polyps to adenocarcinomas. DNA ploidy data were then compared with...
Sauder CA, Koziel JE, Choi M, Fox MJ, Grimes BR, Badve S, Blosser RJ, Radovich M, Lam CC, Vaughan MB, Herbert BS, Clare SE. Phenotypic plasticity in normal breast derived epithelial cells. BMC Cell Biol. 2014 Jun 10; 15:20 ...
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Background and Aims. Genome duplication is widely acknowledged as a major force in the evolution of angiosperms, although the incidence of polyploidy in different floras may differ dramatically. The Greater Cape Floristic Region of southern Africa is one of the worlds biodiversity hotspots and is considered depauperate in polyploids. To test this assumption, ploidy variation was assessed in a widespread member of the largest geophytic genus in the Cape flora: Oxalis obtusa.. Methods. DNA flow cytometry complemented by confirmatory chromosome counts was used to determine ploidy levels in 355 populations of O. obtusa (1014 individuals) across its entire distribution range. Ecological differentiation among cytotypes was tested by comparing sets of vegetation and climatic variables extracted for each locality.. Key Results. Three majority (2x, 4x, 6x) and three minority (3x, 5x, 8x) cytotypes were detected in situ, in addition to a heptaploid individual originating from a botanical garden. While ...
Mutations of the KRAS2 protoncogene and inactivation of the TP53 oncosuppressor gene have been suggested to contribute to chromosomal instability (CIN) and aneuploidy in colorectal cancer (CRC). Previous work has also shown that the degree of DNA ploidy [DNA index (DI)], as obtained by flow cytometry in CRC, is non-randomly distributed and, in particular, that DI near-diploid and near-triploid values are well separated by a low-probability valley region. At present, it is not known whether a relationship exists between DI and the mutational status of KRAS2 and TP53. Multiple samples obtained from 35 human sporadic CRCs have been used to provide nuclei suspensions for flow cytometric analysis and sorting of specific DI subpopulations. Sorted nuclei were then used to analyze the high-microsatellite-instability (MSI-H) phenotype and the mutation spectrum of the KRAS2 and TP53 genes. A single MSI-H case was detected. There were 6 DNA diploid (DI = 1) and 29 aneuploid (DI not equal 1) CRCs, with the DI
Clinical experience has shown that mammary carcinomas can be classified according to their type of progression into slow-growing and fast-growing ones, where the terms
Background: DNA ploidy has been shown to have prognostic significance in patients with breast cancer. Studies in the past have mainly utilized flow cytometry (FCM) for measuring DNA ploidy. However FCM has several disadvantages, the instrument cannot distinguish benign from malignant cells and it cannot be performed on small tumor samples. The relationship between DNA ploidy and biomarker expression in breast cancer has not been well studied. Recently, gene expression analysis has demonstrated distinct subtypes of breast cancer. Expression of ER, PR and Her2 by IHC has been used as a surrogate tool for the molecular classification of breast cancer. Aim: To determine the relationship between DNA ploidy, biomarkers (ER, PR, HER2, Ki67 and p53) expression and molecular subtypes of invasive breast cancer (IBCA) using image analysis.. Design: DNA analysis was performed on Feulgen stained sections from the same tumor block used for biomarker analysis. DNA indices and ploidy were analyzed using the ...
Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of Ser-464 of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at Ser-15 and Ser-392 and is recruited to the CDKN1A/WAF1 promoter to participate to transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced
TY - JOUR. T1 - IL VALORE PROGNOSTICO DELLA PLOIDIA, DELLINDICE PROLIFERATIVO E DEI RECETTORI PER LEPIDERMAL GROWTH FACTOR NEL CANCRO DELLO STOMACO OPERATO. ANALISI DI 130 CASI. AU - Santoro, E.. AU - Zupi, G.. AU - Vecchione, A.. AU - Carboni, M.. AU - Carlini, M.. AU - Catarci, M.. AU - Giannarelli, D.. AU - DAgnano, I.. AU - Santoro, R.. AU - Garofalo, A.. PY - 1995. Y1 - 1995. N2 - DNA ploidy, proliferative index (PI) and EGF-R espression of 130 gastric adenocarcinomas submitted to surgical treatment during the last ten years, were evaluated and related to the usual prognostic variables, such as TNM, stage, grading and histology of the tumor. DNA ploidy and PI were obtained through flow cytometry whereas EGF-R was evaluated with immunostaining. Diploid tumors were observed in 52% of cases and aneuploid in the remaining 48%. Low PI was present in 58% and high PI in 42% of cases. EGF-R was expressed in 69% of cases. All the three biologic variables were significantly related to T and ...
login1$ vcfutils.pl qstats test.raw.vcf QUAL #non-indel #SNPs #transitions #joint ts/tv #joint/#ref #joint/#non-indel 186 1011 1011 757 0 2.9803 0.0000 0.0000 2.9803 142 2036 2036 1441 0 2.4218 0.0000 0.0000 2.0059 122 3091 3091 2156 0 2.3059 0.0000 0.0000 2.1029 109 4177 4177 2925 0 2.3363 0.0000 0.0000 2.4259 99.5 5237 5237 3647 0 2.2937 0.0000 0.0000 2.1361 92 6273 6273 4354 0 2.2689 0.0000 0.0000 2.1489 85.5 7328 7328 5105 0 2.2964 0.0000 0.0000 2.4704 79 8352 8352 5811 0 2.2869 0.0000 0.0000 2.2201 74 9369 9369 6497 0 2.2622 0.0000 0.0000 2.0725 69 10553 10553 7311 0 2.2551 0.0000 0.0000 2.2000 65 11782 11782 8176 0 2.2673 0.0000 0.0000 2.3764 61 13059 13059 9090 0 2.2902 0.0000 0.0000 2.5179 57 14280 14280 9945 0 2.2941 0.0000 0.0000 2.3361 53 15301 15301 10656 0 2.2941 0.0000 0.0000 2.2935 48.8 16323 16323 11347 0 2.2803 0.0000 0.0000 2.0876 45 17460 17460 12122 0 2.2709 0.0000 0.0000 2.1409 41.8 18639 18639 12899 0 2.2472 0.0000 0.0000 1.9328 39.8 19660 19660 13572 0 2.2293 0.0000 0.0000 ...
... by Ventana Medical Systems, Inc. is intended to determine ploidy status of chromosome 17 via chromogenic SISH in breast cancer specimens.
DNA quantification and cell cycle phases study provide relevant clinical information for prognostic, evaluation and follow-up of patients with solid tumors or hematological malignancies. It has been proved that DNA ploidy is characteristic of malignant neoplasm for some type of diseases and can be associated with better or worse prognosis ...
Hi all, I am calling SNPs in various immortalised cell lines, which are known to be very instable - hence the ploidy is not known.
Looking for online definition of DNA ploidy analysis in the Medical Dictionary? DNA ploidy analysis explanation free. What is DNA ploidy analysis? Meaning of DNA ploidy analysis medical term. What does DNA ploidy analysis mean?
article{28d6110c-5d57-42d8-9243-f7ea1c6d6398, abstract = {This analysis of DNA-ploidy heterogeneity in advanced gastric carcinomas is consistent with the hypothesis of the emergence of a single aneuploid cell clone as a crucial mechanism in the progression from early gastric carcinoma to advanced gastric cancer. The prognostic value of DNA-ploidy in gastric cancers has been a matter of controversy. Tumour DNA-ploidy heterogeneity, the presence within the same tumour of multiple stemlines differing in DNA content, has been described in various tumours including gastric cancers. The occurrence of such heterogeneity has been accepted as an explanation for the divergent DNA-ploidy results in this type of tumours. A previous study of early gastric cancers suggested that in pure diploid superficial carcinomas, genetic instability might lead to a cell clone which has undergone a ploidy shift and is more aggressive. If so, this would initially result in DNA-ploidy heterogeneity. Proliferative dominance ...
Endopolyploidy (increased cell ploidy) occurs during normal development in many eukaryotes. In higher plants, endopolyploidy is usually the result of endoreduplication - endonuclear DNA replication that produces chromosomes with multivalent chromatids. According to the karyoplasmic ratio theory, a cells cytoplasmic volume is proportional to its nuclear DNA content. On p. 3817, Christian Chevalier and co-workers test this theory by analysing the structure of endoreduplicated nuclei in tomato fruit, which reach very high ploidy levels during their development. The researchers show that endopolyploidy in tomato pericarp (the fleshy part of the fruit) leads to the formation of polytene chromosomes. Pericarp nuclei, they report, have a complex structure in which numerous deep grooves are filled with mitochondria and in which there is a fairly constant ratio between nuclear surface area and the nuclear volume. Finally, they provide the first direct evidence that endoreduplication triggers enhanced ...
Aneuploidy is a well recognised feature of human tumours, but the investigation of its biological and clinical significance has been hampered by technological constraints. Quantitative DNA analysis reflects the total chromosomal content of tumour cells and can now be determined rapidly and reliably using flow cytometry; this has resulted in renewed interest in its potential clinical applications. This article reviews the accumulating evidence that tumour ploidy reflects the biological behaviour of a large number of tumour types and that diploid tumours in particular have a relatively good prognosis. The measurement of tumour ploidy is likely to become a valuable adjunct to the clinical and histopathological assessment of cancers.. ...
Authors: Abad, Mar , Ciudad, Juana , Rincon, Manuel R. , Silva, Isabel , Paz‐Bouza, José I. , Lopez, Antonio , Alonso, Alberto G. , Bullon, Agustin , Orfao, Alberto Article Type: Research Article Abstract: In the present study the prognostic value of both DNA ploidy and the proliferative activity of tomour cells were studied in a series of 76 consecutive patients suffering from gastric tumours. DNA ploidy and the proliferative index (as measured by the percentage of S‐phase cells) were determined by flow cytometry using fresh tumour specimens. The presence of DNA aneuploid clones by flow cytometry was detected in 62% of the cases (mean DNA index of 1.63\pm 0.46 ; range 1.08-2.92), the mean proportion of S‐phase cells being of 18.4\pm 11.5\% . In comparison with diploid cases, aneuploid tumours …showed a higher proliferative activity (cases with more than 15% S‐phase cells: 18.4% versus 6.1%, p=0.0001 ) as well as a higher incidence of node involvement (95% versus 68%, p=0.001 ). By ...
Historically, genetic maps in high-level autopolyploids have been constructed using only alleles present in one homolog, called single-dose or simplex markers (Wu et al. 1992; Sorrells 1992). In a full-sib population, these markers segregate in a 1:1 ratio (if they are present only in one parent), or in a 1:2:1 ratio (if present in both parents, also called double simplex). Given this level of simplification, it is possible to use the five-step procedure coupled with a standard software suitable for diploid populations. Nevertheless, it is well accepted that the use of single-dose markers imposes limitations on the construction of adequate genetic maps. These approaches sub-sample the genome (Hackett et al. 2013; Garcia et al. 2013), which precludes further consideration of multiallelic effects in models for QTL mapping and subsequent studies. Moreover, there is low statistical power to detect linkage when markers are in repulsion phase configurations (Wu et al. 1992; Ripol et al. 1999). ...
The role of ploidy and ploidy shifts in the emergence of pathogenic fungi is now beginning to be appreciated. The degree to which such ploidy changes drive the emergence of new fungal threats is not yet certain; however, it is clear that ploidy shifts and aneuploidy can promote the acquisition of altitude on the fitness landscape, even if the aneuploid state is transient. An example is C. neoformans, which has emerged from an environmental saprophyte to a pathogen of global importance [58]. Very large and polyploid (4n, 8n and 16n) cryptococcal cells, termed titan cells, were recently discovered and shown to be better able to tolerate oxidative and nitrosative stress [59], to prevent phagocytosis and contribute to dissemination to the central nervous system (CNS). In addition, the large size of titan cells protects them from phagocytosis by immune cells. Titan cells have been proposed to promote persistence in the host based on their prevalence in chronic lung infections [60]. Titan cells give ...
Semantic Scholar extracted view of The DDD-method in the cytophotometric quantitative estimation of protein-bound sulfhydryl groups in palatal smears. by Peter A. E. Sillevis Smitt et al.
Cell cycle checkpoints are biochemical signal transduction pathways that prevent downstream events from being initiated until upstream processes are completed. We analyzed whether the p53 or pRb tumor suppressors are involved in a checkpoint(s) that prevents DNA rereplication in the presence of drugs that interfere with spindle assembly. Normal mouse and human fibroblasts arrested with a 4N DNA content when treated with nocodazole and Colcemid, whereas isogeneic p53-deficient or pRb-deficient derivatives became polyploid. Flow cytometric and cytogenetic analyses demonstrated that the polyploidy resulted from genomewide rereplication without an intervening mitosis. Thus, p53 and pRb help maintain normal cell ploidy by preventing DNA rereplication prior to mitotic division.. ...
Intratumor heterogeneity can lead to underestimation of the tumor genomics landscape portrayed from single tumor-biopsy samples and may present major challenges to personalized-medicine and biomarker development. Intratumor heterogeneity, associated with heterogeneous protein function, may foster tu …
The flow acetone staining technique (FAST) allows one to concurrently study physical cell features revealed by light‐scatter analysis, surface/nuclear phenotypes, and cellular DNA content
Karyotyping analyzes revealed that the chromosome numbers of the three cell systems ranged from 60 to 67 with 3n ploidies, and that there were many structural aberrations, such as del(11)(q13), del(22)(q13), add(2)(p11), add(7)(q22), extra copies for chromosomes 1, 2, 3, 5, 7, 9, 10, 11, 12, 16, 20, and 22, der(9)t(9;13)(p13;q12)add(9)(q34), and der(13;21)(q10;q10), which were shared by the three cell systems, while der(19)t(11;19)(q13;p13) was found in MINT1 and MINT3 ...
My mom came for a visit and she helped me take pictures of diploid (2 sets of chromosomes) and haploid (one set of chromosomes) individuals that had settled onto some PVC posts I placed out in the mudflat last year as well as weighing the algae so we can calculate the water content. With Dr. Courtney Murren, we will be analyzing these images to see whether there are differences between the three types of individuals we find on the mudflats here in Charleston. This might give us some insight into why one of the ploidies (the number of sets of chromosomes within an organisms) is more dominant than the other. Check back for updates on these small project that Courtney and I are doing ...
Background & Aims Intratumor heterogeneity is a common feature of colorectal cancers (CRC). and enough for inducing EMT, invasion, and migration in epithelial-like CRC cells. In principal CRCs, increased appearance was connected with mutation and microRNA-192/215 down-regulation. Significantly, increased appearance in CRCs correlated with improved tumor development and poor individual survival. Conclusions together Taken, our results present that CRC cells promote tumor development via secreting NID1, which induces EMT in neighboring tumor cells. Significantly, the disturbance of p53 with this paracrine signaling between tumor cells?might?donate to tumor suppression critically. (had been up-regulated on the amount of messenger RNA (mRNA) appearance in DLD1, HCT15, HCT116, and LoVo cells following the addition of CM from mesenchymal-like CRC cell lines (Body?1and 1and and in DLD1, HCT15, HCT116, LoVo, HT29, and Caco2 cells cultured for 96 hours in CM extracted from SW480 or SW620 cells. Mean ...
Archival paraffin embedded material was used to examine whether additional quantitative criteria would be helpful to discriminate between histologically benign and malignant rat mammary tumours. To this end nuclear DNA content expressed as DNA ploidy index (DI) was measured using flow cytometry (FCM). A total of 63 benign and malignant mammary tumours were investigated. Thirteen out of 38 (34%) mammary carcinomas were DNA aneuploid against 0 out of 25 benign mammary tumours. Aneuploidy was not significantly increased in tumours showing histological signs of greater malignancy such as cribriform-comedo type or invasive growth. In addition to DI other quantitative criteria indicative for malignancy, such as mitotic count and nuclear morphometric characteristics, were estimated in 24 benign and malignant tubulopapillary tumours, a category where the histological classification may be difficult. It appeared that five out of nine noninvasive tubulopapillary carcinomas and six out of seven invasive ...
Hi Philipp, You can get devel versions of R at ftp://ftp.stat.math.ethz.ch/Software/R/. I believe the idea has always been that BioC devel (i.e., 2.6) works with R devel; iFlow is in BioC 2.6 so that should work. However, iFlow may not necessarily need the latest R. I havent tried the latest version but I have been playing with iFlow back in 2009 using R 2.9 or so. You can install iFlow from sources from the SVN repository (https://hedgehog.fhcrc.org/bioconductor/trunk/madman/Rpacks) user readonly, password readonly. To install it, run the R CMD INSTALL ,path to your iFlow,. The only pain with this approach is that you will have to manually resolve all the dependencies. Good luck. Cheers, Josef , Date: Mon, 08 Feb 2010 11:52:41 +0100 , From: Philipp Meng ,philipp.meng at meduniwien.ac.at, , To: Martin Morgan ,mtmorgan at fhcrc.org, , Cc: bioconductor ,bioconductor at stat.math.ethz.ch, , Subject: Re: [BioC] Installing problems of Flow Cytometry on Ubuntu , Karmic , Message-ID: , ...
Flow cytometry (or FACS) is a technology that allows the rapid measurement of fluorescently labelled samples and common applications include cell cycle, ploidy analysis, proliferation, apoptosis, immunophenotyping, live/dead assays and expression analysis. This technology can also be used to separate out individual cells to either improve transfection efficiencies or create stable cell lines. The facility is located on Level 5 (5.13) of the L.C.Miall building. Please follow the links on the left hand side to find out more about the facility, the equipment available and how it can help your research.. Upcoming course. In conjunction with InCytometry (www.incytometry.co.uk) the facility will be re-running the one day course on the Basics of Flow Cytometry during this semester. Please e-mail the facility manager if you are interested, [email protected] Numbers are very limited, and there will be a small charge for attending.. Please note this course is not currently scheduled, and will ...
DNA Measurement Protocol (Flourimeter Protocol) To use the flourimeter in order to obtain DNA measurements, a set of steps were followed. First, the machine was set up. The device used to take photographs of the DNA, a phone, was set in place. The flourimeter was lined up in a position so that accurate photographs of the drops could be taken. A glass slide was put in place on the flourimeter so that light would be able to reach the sample and pass through it. A single drop of the DNA sample was then placed onto a slide. It is important to note that each drop (of each sample) was placed on the slide separately (one at a time). From there, the green dye (GoTaq Master Mix) was added. A photo was taken of the first DNA sample. The sample was removed and disposed of properly before the next sample was put in place on the slide. The slide was moved slightly so that the drop would not be contaminated with the DNA from the previous sample. For each sample, two drops of green dye were added and a photo ...
DNA Measurement Protocol (Flourimeter Protocol) To use the flourimeter in order to obtain DNA measurements, a set of steps were followed. First, the machine was set up. The device used to take photographs of the DNA, a phone, was set in place. The flourimeter was lined up in a position so that accurate photographs of the drops could be taken. A glass slide was put in place on the flourimeter so that light would be able to reach the sample and pass through it. A single drop of the DNA sample was then placed onto a slide. It is important to note that each drop (of each sample) was placed on the slide separately (one at a time). From there, the green dye (GoTaq Master Mix) was added. A photo was taken of the first DNA sample. The sample was removed and disposed of properly before the next sample was put in place on the slide. The slide was moved slightly so that the drop would not be contaminated with the DNA from the previous sample. For each sample, two drops of green dye were added and a photo ...
Screening for haploids, diploids, triploids and polyploids Ploidy and genome size analysis in less than two minutes Detection of anisoploids, allop...
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Nous avons trouvé que le contenu en ADN nucléaire varie peu chez les lamproies. Dans les 3 familles principales ce contenu représente en moyenne le 40% de celui de lhomme. Il existe une corrélation entre le contenu en ADN et le caryotype dans presque tous les genres.. ...
Uses of Flow Cytometry 1. Multicolour analysis Cell Cycle and Proliferation... 3 a. Analysis of Cellular DNA Content... 4 b. Cell Proliferation Assays Immunology Apoptosis... 7
Describes a cell or organism which has more than the normal total number of chromosomes. For example, humans normally have 46 chromosomes per cell - but...
Affiliation:公益財団法人ルイ・パストゥール医学研究センター,その他部局等,研究員(移行), Research Field:Human pathology,Radiation science,Experimental pathology, Keywords:大腸癌,癌遺伝子,大腸腺腫,モノクローナル抗体,小腸腺化生,分化抗原,粘液組成,核DNAパターン,印環細胞癌,DNA ploidy, # of Research Projects:16, # of Research Products:0
Bits of genetic material in rivers make it possible to detect the organisms living in them - without having to collect these and examine them under the microscope. Researchers at Eawag, the ETH and the EPFL have now developed a computer model that with the help of single DNA measurements even simulates exactly where and how often the species are present in bodies of water. ...
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A mutated clone with different ploidy levels in epidermis cells, 5/74/2, of the haploid `Kleiner Liebling` of Pelargonium zonale was investigated to answer the question that how the different ploidy levels were generated. Such a variability did not appear in L2- and L3-derived cells. Consequently, clone 5/74/2 is a periclinal cytochimera with a mixed ploidy epidermis. This type of cytochimera with different ploidy levels in epidermis has not been reported up to now. The epidermis of the blistered leaf or of the hairy leaf is polyploid with different ploidy levels, like the epidermis of the shoots with blistered and hairy leaves. Epidermis cells of normal shoots are diploid. The morphologically blistered leaf surface seems to be the result of a somatic variability in epidermis. Histological investigations of clone 5/74/2 showed two different ways of development of the somatic variability: the cells of the L1 in the apical meristem were already polyploid and the cells in the apical meristem were ...
Background: DNA ploidy analysis of cervical intraepithelial neoplasia (CIN) and invasive cervical cancer samples by flow cytometry (FCM) has been established as an aid to prognostic assessment. Liquid based cytology (LBC) increases diagnostic specificity by using ancillary techniques that provide information beyond morphology. The present study was undertaken to assess DNA ploidy in LBC samples as an adjunct for early detection of cervical pre-cancer and cancer. Methods: DNA ploidy assessment was performed on LBC samples of 50 cases and 31 controls. Cell pellets were obtained by centrifugation and stained with Telford reagent. At least 20,000 R1 gate (G0-G1) events were acquired on a BD FACSCalibur by using a 575±10 nm filter. Results: Mean diploid G1 values were lowered significantly (p|0.01) while diploid S values were significantly elevated (p|0.01) in both high grade squamous intraepithelial lesions (HSILs) and squamous cell carcinomas (SCCs) as compared to controls. Receiver operating curve (ROC
The surface exposure of CRT and ERp57 was increased in CT26 clones derived from cells transiently exposed to nocodazole that contained close to twice the DNA content of parental cells (which we refer to as "hyperploid" cells), although the surface expression of most other membrane proteins was unaltered (Fig. 1D and fig. S8). This hyperploidy-associated increase in CRT exposure was also observed in mouse Lewis lung carcinoma (LLC) and fibrosarcoma MCA205 cells, as well as in human cancer cell lines (fig. S9). As compared to their parental counterparts, hyperploid clones exhibited constitutive PERK and eIF2α phosphorylation (Fig. 1E). Interruption of the CRT exposure pathway reduced the clonogenic potential of hyperploid cells (Fig. 1, F and G), suggesting a functional link between the ER stress-associated CRT exposure pathway and the fitness of hyperploid cells.. Because hyperploidization is linked to CRT exposure, we wondered whether cancer cells with increased DNA content might be subjected ...
The surface exposure of CRT and ERp57 was increased in CT26 clones derived from cells transiently exposed to nocodazole that contained close to twice the DNA content of parental cells (which we refer to as "hyperploid" cells), although the surface expression of most other membrane proteins was unaltered (Fig. 1D and fig. S8). This hyperploidy-associated increase in CRT exposure was also observed in mouse Lewis lung carcinoma (LLC) and fibrosarcoma MCA205 cells, as well as in human cancer cell lines (fig. S9). As compared to their parental counterparts, hyperploid clones exhibited constitutive PERK and eIF2α phosphorylation (Fig. 1E). Interruption of the CRT exposure pathway reduced the clonogenic potential of hyperploid cells (Fig. 1, F and G), suggesting a functional link between the ER stress-associated CRT exposure pathway and the fitness of hyperploid cells.. Because hyperploidization is linked to CRT exposure, we wondered whether cancer cells with increased DNA content might be subjected ...
AMONG the genetic and epigenetic changes to genomes, changes in ploidy are the most drastic, and as such, polyploidy is not tolerated by most animal species (Li et al. 2009a). A recent study of tetraploid yeast suggests that the deleterious effects of ploidy change are due to the uncoordinated scaling of the spindle pole body, spindle, and kinetochore, thus resulting in genetic instability (GIN) (Storchova et al. 2006). However, ploidy changes occur in every sexual cycle of all eukaryotes and are associated with the inclusion or exclusion of an entire set of chromosome homologs that significantly alters the DNA repair capacity. Little is known about whether DNA damage response is regulated differently in haplophase and diplophase during sexual cycles.. DNA replication stress, induced by oncogene activation, genotoxic stress, or defects in the DNA replication machinery, is believed to cause GIN that accelerates tumorigenesis (Halazonetis et al. 2008). However, DNA replication stress does not ...
TY - JOUR. T1 - Cytophotometric Studies of DNA in Circulating Erythrocytes of Brook Trout Exposed to Acid pH. AU - Allen, Sharon S. AU - Mitchell, R. B.. AU - Anthony, Adam. PY - 1969. Y1 - 1969. M3 - Article. VL - 43. SP - 191. EP - 194. JO - Proceedings of the Pennsylvania Academy of Science. JF - Proceedings of the Pennsylvania Academy of Science. SN - 0096-9222. ER - ...