TY - JOUR. T1 - Protective effect of glutathione on the cytotoxicity caused by a combination of aluminum and iron in suspension-cultured tobacco cells. AU - Yamaguchi, Yukiko. AU - Yamamoto, Yoko. AU - Ikegawa, Hiroshi. AU - Matsumoto, Hideaki. PY - 1999/3. Y1 - 1999/3. N2 - The role of endogenous glutathione (GSH) in the protection of suspension-cultured tobacco cells from aluminum (Al) toxicity was examined. Cells at the logarithmic phase of growth were treated with or without A1 in nutrient medium prepared without P(i) and EDTA. In the absence of A1, total GSH content (including oxidized glutathione [GSSG]) increased gradually. In the presence of Al, the increase of GSH was repressed. This effect was observed before the loss of plasma membrane integrity and the loss of cell viability. In contrast, GSSG content in cells increased in the presence of A1. GSH-deprived cells were prepared by culturing cells with buthionine sulfoximine (an inhibitor of γ-glutamylcysteine synthetase) for 24 h. ...
Read "Manipulation of the napin primary structure alters its packaging and deposition in transgenic tobacco (Nicotiana tabacum L.) seeds, Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
The cell line of tobacco cultivar BY-2 (Nicotiana tabacum L. cv. Bright Yellow No.2) is one of the most commonly used in plant cell biology. The purpose of this study was to follow the intracellular localization of protein of unknown function from maize (ZmMAB1) during the cell cycle. Synchronization of the cell cycle of tobacco BY-2 cells in the liquid medium was achieved by careful selection of initial dilution, degree of differentiation and subculturing period. In the cell culture which was left two weeks without subculturing, cells became differentiated and mature but after resubculturing in fresh medium, they began to divide, and the third day they reach the highest mitotic index. Visualization of microtubules allowed the identification of particular stages of mitosis, and that was achieved by flourescently labeled protein MAP-4. The maize protein ZmMAB1 labeled with red fluorescent protein showed its colocalization with the microtubules during division ...
Read "Identification of a Nicotiana plumbaginifolia Plasma Membrane H+-ATPase Gene Expressed in the Pollen Tube, Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Search Indian Bright Yellow Sulphur Manufacturers and Suppliers Details - Contact to Bright Yellow Sulphur Exporters in India, Bright Yellow Sulphur Wholesalers, Bright Yellow Sulphur Distributors and Traders from India.
Ion fluxes and the production of reactive oxygen species (ROS) are early events that follow elicitor treatment or microbial infection. However, molecular mechanisms for these responses as well as their relationship have been controversial and still largely unknown. We here simultaneously monitored the temporal sequence of initial events at the plasma membrane in suspension-cultured tobacco cells (cell line BY-2) in response to a purified proteinaceous elicitor, cryptogein, which induced hypersensitive cell death. The elicitor induced transient rise in cytosolic Ca2+ concentration ([Ca2+]cyt) showing two distinct peaks, followed by biphasic (rapid/transient and slow/prolonged) Cl- efflux and H+ influx. Pharmacological analyses suggested that the two phases of the [Ca2+]cyt response correspond to Ca2+ influx through the plasma membrane and an inositol 1,4,5-trisphophate-mediated release of Ca2+ from intracellular Ca2+ stores, respectively, and the [Ca2+]cyt transients and the Cl- efflux were ...
Proteinase inhibitors and thionins are among the many proteins thought to have a role in plant defence against pests and pathogens. Complementary DNA clones encoding the precursors of a multi-domain proteinase inhibitor from Nicotiana alata Link et Otto (NA-PI) (Mr approximately 43 000) and a β-hordothionin (β-HTH) (Mr approximately 13 000) from barley, were linked to constitutive promoters and subsequently transferred by Agrobacterium-mediated transformation into tobacco. The NA-PI and β-HTH precursor proteins were synthesised and post-translationally processed in transgenic tobacco and accumulated as polypeptides of apparent size Mr approximately 6000 and Mr approximately 8500, respectively. The na-pi and β-hth genes were stably inherited for at least two generations. Transgenic tobacco plants containing the highest amounts of NA-PI and β-HTH were crossed to produce plants containing both genes. Helicoverpa armigera (tobacco budworm) larvae that ingested transgenic tobacco leaves ...
Sigma-Aldrich offers abstracts and full-text articles by [Nicolas Amelot, Audrey Carrouche, Saïda Danoun, Stéphane Bourque, Jacques Haiech, Alain Pugin, Raoul Ranjeva, Jacqueline Grima-Pettenati, Christian Mazars, Christian Briere].
This institute contributes to the education of students in Graduate School for Environmental and Life Science (Masters and Doctors Degree Course of Okayama University).
Last night I had bright yellow liquid (watery) come out of my right nostril. I went to the doctor today but they dont know what it is. I have had clear watery liquid come out before like this but ne...
Abstract Using single-copy conserved ortholog set (COSII) and simple sequence repeat (SSR) markers, we have constructed two genetic maps for diploid Nicotiana species, N. tomentosi..
The tobacco plant could be used to fight cancer cells in humans in the future.. A molecule found in a variety of flowering tobacco plant could hold the key to beating cancer in years to come.. Dr. Mark Hulett, lead researcher at La Trobe University, Australia where the discovery was made said: “There is some irony in the fact that a powerful defence mechanism against cancer is found in the flower of a species of tobacco plant, but this is a welcome discovery.†According to Dr. Hullet, the main problem with current treatments is that they attack cells indiscriminately, whether they are cancerous or not.. NaD1, the protein responsible for defending the tobacco plant against fungi and bacteria uses a different approach, which may prove revolutionary.. The molecule works by using a pincer-like structure to latch onto lipids in the membrane of cancer cells, causing them to rupture and explode.. Unlike current cancer treatment, NaD1 targets affected cells whilst leaving healthy cells ...
Nicotiana Tabacum, Tobacco. This agent is too irregular in action to be a safe internal remedy. Squibbs Nicotine is a nerve sedative and relaxant of value in strychnine poisoning in doses of I -64 to...
Tobacco etch virus ATCC ® PV-69™ Designation: Application: Host plant from which virus was purified: Nicotiana tabacum cv. Plant research
The first thing you should do is closely examine the "head" of the cigar - this is the closed end that needs to be clipped. Almost all have what is called a "cap" - a bit of tobacco leaf used to close of the end - you should be able to see how far down the length of the cigar the cap goes by inspection. Typically only a 1/4″ - 3/8″ or so; sometimes much less, and on figurado shapes sometimes quite longer. Anyhow wherever the cap stops is your cutting limit - cut beneath the caps line or even too close and your cigar will start to unwravel, and as you pointed out this is extremely unpleasant. Typically I cut the minimal possible while trying to open approx. 75%-85% of the cigar ends surface area. Sometimes this means a cut as little as 1/32″ down, where other times almost 3/8″ - it depends entirely on the individual cigars roll and cap construction. The single bladed cheapie cutters that most newbies are given or buy for $3 typically do a very poor job of clipping the cap, and result ...
DARVISHZADEH, Reza et al. Genetic variation in oriental tobacco (Nicotiana tabacum L.) by agro-morphological traits and simple sequence repeat markers. Rev. Ciênc. Agron. [online]. 2013, vol.44, n.2, pp.347-355. ISSN 1806-6690. https://doi.org/10.1590/S1806-66902013000200018.. The objectives of this study were to assess genetic diversity and determine differences between several oriental tobacco genotypes by examining both agro-morphological traits and molecular markers. Simple lattice design with two replications was used to evaluate 100 oriental tobacco genotypes. Analysis of variance manifested that there is high level of genetic diversity in oriental-type tobaccos based on morphological traits including number of leaf, days to 50% flowering, leaf length, leaf width, leaf fresh weight, leaf dry weight, stem height and stem girth. Classification of genotypes using agro-morphological data by means of un-weighted pair-group method using arithmetic average (UPGMA) algorithm based on squared ...
TY - JOUR. T1 - In vitro translation products of mrnas derived from TMV-infected tobacco exhibiting a hypersensitive response. AU - Smart, Thomas E.. AU - Dunigan, David D.. AU - Zaitlin, Milton. PY - 1987/6. Y1 - 1987/6. N2 - Expression of the hypersensitive response (HSR) to tobacco mosaic virus (TMV) infection in Nicotiana tabacum L. cv. Xanthi-nc (genotype NN) is controlled by the single dominant "N" gene and is temperature sensitive. TMV-infected Xanthi-nc tobacco plants grown at the HSR-restrictive temperature of 31° for 3 days postinoculation show necrosis approximately 8 hr after the temperature shift to the HSR-permissive temperature of 25°. Both polyribosomal and total cytoplasmic poly(A)-containing RNAs were isolated at various times after the temperature shift from leaves of TMV-infected and mock-infected Xanthi-nc tobacco plants and from TMV-infected Turkish Samsun tobacco plants (genotype nn; systemic for TMV infection). The RNAs were translated in vitro and the products were ...
Bright yellow was all the rage at the 2012 Emmys and has been showing up ever since on the red carpet and in celebrity candids as well. Whether it be Big Bird or mustard, bright yellow is hot right now and I have no doubt it will be a favorite at the upcoming Golden Globes Awards. 2012…
... Knowing how big and elevation of your Childrens bright yellow fantastic banquette chair wooden bookcase blue ticking, now you can consider the type. You can opt for your simple square desk, carved table, fantastic banquette chair table or fold table. Additionally...
How to Choose the Right Moist Snuff for You. Moist snuff is a smokeless tobacco product that is consumed by placing it into your mouth between the lip and the gum. These kinds of tobacco and nicotine products are known as dip, and using...
Learn why meal replacement shakes and protein powders turn your urine bright yellow and if this is cause for concern, plus a healthier smoothie recipe to make instead
Rugged one piece lightweight polyethylene spineboard Twelve large hand holes for easy handling, built in runners, Low profile and X-ray translucent. Junkin Plastic Backboard, Bright Yellow
The RbcS genes encode the small subunits of rubisco; the expression of these genes is controlled in a light-dependent and independent manner. It has been reported that intracellular calmodulin (CaM) is involved in light-dependent RbcS expression. In this report, the role of extracellular CaM in regulating expression of RbcS in darkness was examined. The time course of expression of RbcS-GUS and that of the secretion of CaM in the suspended transgenic tobacco cells in darkness were very similar. Both showed initial increase followed by decline with maximum CaM secretion preceding maximum GUS expression by 24 h. The concentration of CaM in the culture medium is regulated light independently. Purified CaM alone added to the media enhanced RbcS-GUS expression in darkness. The addition of membrane-impermeable CaM inhibitors, such as anti-CaM antiserum or W7-agarose, repressed the expression of RbcS-GUS in darkness, but this inhibitory effect was completely reversed by adding exogenous purified CaM. ...
A comparison of the DNA metabolisms of uninfected and TMV infected excised tobacco leaves, using p(32)-orthophosphate incorporation into the DNA as a measure of its metabolism, indicated that the DNA metabolism is not affected by TMV infection. This result was corroborated by the results of studies on the effect of 5-fluorouracil, a specific inhibitor of DNA synthesis, on the multiplication of TMV in tobacco-leaf discs. Although partial inhibition of TMV multiplication was observed, the absence of inhibition reversal thymidine, indicated that the mechanism of TMV inhibition probably did not involve a specific block of DNA synthesis. Finally unsuccessful attempts were made to see if intact host DNA was necessary for TMV infection by treating tobacco-leaf discs with DNAase ...
Swedish Match develops, manufactures, and sells quality products with market leading brands in the product areas Snus and moist snuff, Other tobacco products (cigars and chewing tobacco), and Lights (matches, lighters, and complementary products).
Keywords: POSTDOCTORAL POSITION ANNOUNCEMENT IN MOLECULAR EVOLUTION OF PLANT VIRUSES PROJECT DESCRIPTION: Applicants are being sought to fill TWO positions in molecular evolution of plant viruses. Position one will involve molecular population biology, epidemiology, and evolutionary mechanisms of peanut stunt cucumovirus. Several virus collections are already available for this study. Position two will involve a study of 1.) the role of viral and host components of the replicase in the generation of variability, and 2.) the role of quasispecies in the biology of cucumber mosaic cucumovirus, including the ability to overcome resistance. More detailed descriptions of both projects are available on request. REQUIREMENTS: A Ph.D. in Molecular Biology, Biochemistry, or related field, with experience in evolution or virology helpful. SALARY and APPOINTMENT: Base salary is US$24,000/year, depending on experience with funding available for 3 years. STARTING DATES: July and August 1995 APPLY TO: Dr. ...
Scanning electron micrograph (SEM), tinted green, of the bacteria Agrobacterium tumifaciens (small bacilli) growing on the surface of cells from the tobacco plant Nicotiana plumbaginifolia. Known as a natural genetic engineer for the ease with which it interferes with its hosts metabolism, this bacterium injects cancer-causing genes (T-DNA) into a plant cell. A tumour develops which, while destructive to the plant, is food to the bacterium. Magnification: X 1070 at 35mm size. Original is BW print B242/01M - Stock Image B242/0002
Saccharification of transgenic tobacco. Saccharification (72 hours at 50°C) of transgenic tobacco pretreated at 110°C, 140°C and 170°C with 100 mg/g cellulo
Nicotiana Tabacum ((Lizard Tail Orinoco x Turtle Foot Tobacco)) 300+ Nicotiana tabacum is a sticky, thick-leaved perennial that can reach up
Tobacco By-2 Cells: From Cellular Dynamics to Omics by Nagata, Toshiyuki available in Hardcover on Powells.com, also read synopsis and reviews. It is our utmost pleasure to present a new book on tobacco BY-2 cells, Tobacco BY-2 Cells: From...
Mottahedeh Tobacco Leaf at Replacements, Ltd., page 1 with links to Tobacco Leaf online pattern registration form, images of more than 425,000 china, crystal, silver and collectible patterns, specialty items for sale, silver hollowware, Christmas ornaments, and much more!
The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.. ...
Binds only to large DNA fragments. Recognizes a DNA fragment carrying 8 copies of box7 motif of the light-induced cab-E promoter of Nicotiana plumbaginifolia. Also recognizes the box7m1 motif.
Tobacco (Nicotiana tabacum L.) plants grown in vitro for 4 weeks on medium containing 10 μmol/L salicylic acid (SA) displayed enhanced tolerance of a 4.5 h heat-shock at 49°C. However, 100 μmol/L SA did not enhance ...
Tobacco: Tobacco, common name of the plant Nicotiana tabacum and, to a limited extent, Aztec tobacco (N. rustica) and the cured leaf that is used, usually after aging and processing in various ways, for smoking, chewing, snuffing, and extraction of nicotine. Various other species in the genus Nicotiana are
Expression of human papillomavirus 18 E6 gene in transgenic tobacco plants / J P Jeon; M A Hyun; D U Kim; S W Jin; Sue Nie Park; H S Kim; W D Yoo; U I Sohn , 1994 ...
In "The Happening," common plants release a neurotoxin that threatens the survival of the human race. This movie premise may be far-fetched, but scientists say summer is a good time to familiarize yourself with toxic plants that can be harmful to your health. ...
I went out this morning looking at my Hawian Lei plants and noticed what looks like very small egg cases on some of the underside of the leaves. Tehey are very small and are bright yellow in color. I touched this and it seemed to be powdering between my fingers I suspect it may be a fungi of some sort. I need some direction in getting rid of it before it harms my plants ...
This is a processing plant of Conoco Phillips Inc in California that can be viewed from an Amtrak train. The bright yellow mountain in the center of the photo is sulfur. It glows fluorescent in the middle of the day ...
See what Xanthi Agathou (xanthiagathou) has discovered on Pinterest, the worlds biggest collection of everybodys favorite things.
The first thing you notice are the sunflowers; loads of em, bright yellow stems painted serenely against a perfect blue sky.Then there are the cheery posters of laughing kids and vibrant seniors,
Crocoite is commonly found as crystals, usually as long prismatic crystals and more rarely as equant crystals, but are most often poorly terminated, and are usually of a bright hyacinth-red color, which are translucent and have an adamantine to vitreous lustre. When fine-grained it can be bright yellow to orange, and some crystals are dark red.
As the tourist trolley bus made its way down Penn Ave, instead of ogling the famous buildings, the tourists seemed more interested in checking out the bright yellow Smart fortwo car parked at the curb, just a few feet from me. I heard the tour guide say something about the building I work in as he stopped at the light, then I saw a middle aged husband and wife pointing at the car, and telling their kids to "Check out that cute little car!" "Wow!" exclaimed a boy of about ten, "Thats just my size!" The light turned green, they were off, and I was left standing, looking at something that I see every day in this city.. ...
پپتیدهای ضدمیکروبی، مولکول‌های قدیمی و حفاظت شده‌ هستند که در مکانیسم‌های دفاعی موجودات زنده مانند باکتری‌ها، جانوران و گیاهان دیده می‌شوند. شناسایی و معرفی پپتیدهای ضدمیکروبی جدید، روشی مقرون به صرفه برای مقابله با میکروب‌های بیماری‌زا و همچنین بهبود مقاومت گونه‌های گیاهان زراعی با استفاده از تکنولوژی DNA نوترکیب است. به این منظور یک سازه ژنی حاوی توالی ژن کد کننده پپتید ضدمیکروبی امیگانان (Omiganan) نوتروفیل گاو پس از همسانه‌سازی به کمک اگروباکتریویوم تومفاشینز به دیسک‌های برگی توتون انتقال داده شد. با روش PCR حضور ژن کد کننده پپتید ضدمیکروبی در ژنوم
Global LED Billboard Market was valued at USD XX(Bn/Mn) in 2017 and is expected to reach USD XX(Bn/Mn) by 2022, growing at a CAGR of XX% between 2017 and 2022
Anabasin je piridinski i piperidinski alkaloid pritutan u duvanskoh biljci Nicotiana glauca, koja je blisko srodna s duvanskom biljkom u širokoj poljoprivrednoj upotrebi (Nicotiana tabacum). Ovaj molekul je strukturalni izomer hemijski srodnog nikotina. Njegova glavna (istorijska) industrijska primena je kao insekticid. Anabasin je prisutan u tragovima u duvanskom dimu, i može se koristiti kao indikator izlaganja.[1] ...
Smoking is rubbish - nearly every part of smoking is bad for the environment, from growing the tobacco plants to people throwing away their used, chemical-filled butts.
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
Weve got 15 years to snuff out a virus that has killed 39 million people. These five approaches are going to help us do it, IntraHealth Internationals Margarite Nathe writes in a guest commentary for World AIDS Day.
n perioada 23-25 noiembrie a.c., eful Statului Major Principal al For elor Aeriene Militare bulgare, general-maior Simeon Hristov Simeonov, efectueaz o vizit de lucru n Rom nia, la invita ia efului Statului Major al For elor Aeriene Rom ne, general-locotenent Gheorghe Catrina.