Rice is highly sensitive to cold stress during reproductive developmental stages, and little is known about the mechanisms of cold responses in rice anther. Using the HiSeq™ 2000 sequencing platform, the anther transcriptome of photo thermo sensitive genic male sterile lines (PTGMS) rice Y58S and P64S (Peiai64S) were analyzed at the fertility sensitive stage under cold stress. Approximately 243 million clean reads were obtained from four libraries and aligned against the oryza indica genome and 1497 and 5652 differentially expressed genes (DEGs) were identified in P64S and Y58S, respectively. Both gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were conducted for these DEGs. Functional classification of DEGs was also carried out. The DEGs common to both genotypes were mainly involved in signal transduction, metabolism, transport, and transcriptional regulation. Most of the DEGs were unique for each comparison group. We observed that there were more differentially
0070] In the near-isogenic line of Oryza sativa L. cultivar Koshihikari containing the sd1 gene derived from Oryza sativa L. cultivar Habataki (Habataki-derived sd1-containing near-isogenic line), the region in which the sd1 gene in a chromosome of Oryza sativa L. cultivar Koshihikari is encoded has been substituted with a chromosome fragment containing a region encoding the sd1 gene derived from Oryza sativa L. cultivar Habataki. The Habataki-derived chromosome fragment contained in the Habataki-derived sd1-containing near-isogenic line is not particularly limited as long as it contains a region in which the sd1 gene is encoded, and may contain only the region in which the sd1 gene is encoded, and a gene present in the proximity of the sd1 gene, together with the sd1 gene, may also be inserted into Oryza sativa L. cultivar Koshihikari. FIG. 1 shows a DNA marker (SNP) of approximately 38.11 Mbp in which the sd1 gene in the chromosome 1 of rice is encoded. A length of the Habataki-derived ...
Cytoplasmic male sterility is total or partial male sterility in plants as the result of specific nuclear and mitochondrial interactions. Male sterility is the failure of plants to produce functional anthers, pollen, or male gametes The first documentation of male sterility was by Joseph Gottlieb Kölreuter, who observed anther abortion within species and specific hybrids. Cytoplasmic male sterility has now been identified in over 150 plant species. It is more prevalent than female sterility, either because the male sporophyte and gametophyte are less protected from the environment than the ovule and embryo sac, or because it results from natural selection on mitochondrial genes which are maternally inherited and are thus not concerned with pollen production. Male sterility is easy to detect because a large number of pollen grains are produced and are easily studied. Male sterility is assayed through staining techniques (carmine, lactophenol or iodine), while detection of female sterility is by ...
337S is a novel bi-pole-photo-thermo-sensitive genic male sterile line in wheat, and sensitive to both long day length/high temperature and short day length/low temperature condition. Although the regulatory function of MicroRNAs (miRNAs) in reproductive development has been increasingly studied, their roles in pre-meiotic and meiotic cells formation of plants have not been clearly explored. Here, we explored the roles of miRNAs in regulating male sterility of 337S at short day length/low temperature condition. Small RNA sequencing and degradome analyses were employed to identify miRNAs and their targets in the 337S whose meiotic cells collapsed rapidly during male meiotic prophase, resulting in failure of meiosis at SL condition. A total of 102 unique miRNAs were detected. Noticeably, the largest miRNA family was MiR1122. The target CCR4-associated factor 1 (CAF1) of miR2275, a subunit of the Carbon Catabolite Repressed 4-Negative on TATA-less (CCR4-NOT) complex, contributes to the process of early
Cytoplasmic male sterility (CMS) is a maternally inherited trait characterized by the inability to produce functional pollen. The CMS-associated protein Orf507 (reported as Orf456 in previous researches) was previously identified as a candidate gene for mediating male sterility in pepper. Here, we performed yeast two-hybrid analysis to screen for interacting proteins, and found that the ATP synthase 6 kDa subunit containing a mitochondrial signal peptide (MtATP6) specifically interacted with Orf507. In addition, the two proteins were found to be interacted in vivo using bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation (Co-IP) assays. Further functional characterization of Orf507 revealed that the encoded protein is toxic to bacterial cells. Analysis of tissue-specific expression of ATP synthase 6 kDa showed that the transcription level was much lower in anthers of the CMS line than in their wild type counterparts. In CMS plants, ATP synthase activity and content were ...
Mitochondrial genomes encode only a fraction of the genetic information required for their biogenesis and function. Consequently, a large number of genetic and biochemical features present in plant mitochondria arose in the context of coevolution and coordinated gene functions between the mitochondrial and nuclear genomes (Mackenzie and McIntosh, 1999). Cytoplasmic male sterility (CMS) is a widespread phenomenon observed in ,150 flowering plant species (Laser and Lersten, 1972). CMS is a maternally inherited trait and is often associated with unusual open reading frames (ORFs) found in mitochondrial genomes, and in many instances, male fertility can be restored specifically by nuclear-encoded, fertility restorer (Rf) genes (Schnable and Wise, 1998). Therefore, CMS/Rf systems are ideal models for studying the genetic interaction and cooperative function of mitochondrial and nuclear genomes in plants.. CMS/Rf systems have long been exploited for hybrid breeding to enhance the productivity of ...
Received 29 Nonember; Accepted 27 December 2014). SUMMARY. Bajra is the fourth most important crop, whose cultivation mainly confines to dry land regions. Gene manipulation using conventional and advanced approaches as well as genetic resource conservation and evaluation is an activity that supports the national crop improvement programme to cope with the biotic and abiotic stresses while maintaining high level of productivity, profitability and quality. Its wild species are tertiary gene pool species as reservoir of important genes such as apomixis, perenniality, fodder characteristics, stress tolerance thereby pearl millet improvement has potential of utilizing interspecific hybridization. Plant pathological research in pearl millet did not receive adequate attention until the F1 hybrids, based on cytoplasmic male sterile line, released for commercial cultivation in India in the mid-1960s and become susceptible to downy mildew in early 1970s. The superiority of hybrids over open pollinated ...
In wheat, there are two methods of Hybrid seed production 1) Cytoplasmic Male Sterility based system 2) Chemical Hybridizing Agents based system
Hybrid Seeds. Genetically-modified (GMO) seeds. Heirloom seeds. The labels often confuse people. What many fail to understand is the fundamental difference between hybrid seeds and GMOs.
Hybrid Seeds. Genetically-modified (GMO) seeds. Heirloom seeds. The labels often confuse people. What many fail to understand is the fundamental difference between hybrid seeds and GMOs.
Alterations of mitochondrial-encoded subunits of the FoF1-ATP synthase are frequently associated with cytoplasmic male sterility (CMS) in plants; however, little is known about the relationship of the nuclear encoded subunits of this enzyme with CMS. In the present study, the full cDNA of the gene TaFAd that encodes the putative FAd subunit of the FoF1-ATP synthase was isolated from the wheat (Triticum aestivum) fertility restorer 2114 for timopheevii cytoplasm-based CMS. The deduced 238 amino acid polypeptide is highly similar to its counterparts in dicots and other monocots but has low homology to its mammalian equivalents. TaFAd is a single copy gene in wheat and maps to the short arm of the group 6 chromosomes. Transient expression of the TaFAd-GFP fusion in onion epidermal cells demonstrated TaFAds mitochondrial location. TaFAd was expressed abundantly in stem, leaf, anther, and ovary tissues of 2114. Nevertheless, its expression was repressed in anthers of CMS plants with timopheevii ...
A number of S male-sterile plants from several shrunken-2 inbred lines were crossed initially with an R138-TR inbred line pollinator carrying the nonrestoring genotype for S sterile cytoplasm. One such cross, involving a male-sterile female parent from inbred line M825, produced, unexpectedly, a number of male-fertile F1 progeny, along with the expected male-sterile off-spring. Pollen records of plants in F2, F3 and F4 progenies in the exceptional pedigree, and of a variety of testcross and backcross progenies from these male-fertile exceptions, indicate that the exceptional male fertility is not attributable to the action of either dominant or recessive nuclear restorer genes. They are, however, consistent with the hypothesis that the event responsible for the appearance of exceptional male-fertile offspring among progeny of the original cross involved a change from male-sterile to male-fertile condition in the cytoplasm of the male-sterile M825 plant involved as the female parent in this ...
0165] A further embodiment of the invention is a backcross conversion of wheat variety SJ909-369. A backcross conversion occurs when DNA sequences are introduced through traditional (non-transformation) breeding techniques, such as backcrossing. DNA sequences, whether naturally occurring or transgenes, may be introduced using these traditional breeding techniques. Desired traits transferred through this process include, but are not limited to nutritional enhancements, industrial enhancements, disease resistance or tolerance, insect resistance or tolerance, herbicide tolerance or resistance, agronomic enhancements, grain quality enhancement, waxy starch, breeding enhancements, seed production enhancements, and male sterility. Descriptions of some of the cytoplasmic male sterility genes, nuclear male sterility genes, chemical hybridizing agents, male fertility restoration genes, and methods of using the aforementioned are known. Examples of genes for other traits include: Leaf rust resistance ...
GD lines can be on chromosome 1, 2 or 3. All KK lines are on chromosome 2. All lines on chromosomes 2 or 3 are balanced. Lines either homozygous lethal or sterile are kept over the balancers (CyO for the 2nd chromosome; TM3, Sb for the 3rd chromosome). Stocks that are viable have been made homozygous. Sterile lines are kept over the balancers. In these stocks there are both homozygous (these flies are sterile) and heterozygous flies for the UAS-IR insertion (over the CyO or TM3). For your RNAi experiments please use flies over the balancers and screen the progeny against the balancer. Lethal lines are kept over the balancers. In these lines all the flies are either over the CyO or TM3,Sb balancer depending on the insertion chromosome. In these stocks homozygous flies (no balancer) survive rarely. Insertions on the 1st chromosome are not balanced, and we keep these strains by crossing the RNAi males to X-compound virgins. In this case only the males have red eyes, never the females. Since these ...
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Cytoplasmic male sterility (CMS) has consistently been associated with the expression of mitochondrial open reading frames (ORFs) that arise from genomic rearrangements. Spontaneous fertility reversion in CMS has been observed in several cases, but a clear understanding of fertility reversion controlled by nuclear genetic influences has been lacking. Here, we identified spontaneous fertile revertant lines for Brassica juncea CMS cytoplasm in which the mitochondrial genome has undergone substoichiometric shifting (SSS) to suppress ORF220 copy number. We placed ORF220, with or without a mitochondrial targeting presequence, under the control of the CaMV35S and AP3 promoters in Arabidopsis to confirm that ORF220 causes male sterility when mitochondrially localized. We found that copy number of the ORF220 gene was altered under conditions that suppress MSH1, a nuclear gene that controls illegitimate recombination in plant mitochondria. MSH1-RNAi lines with increased ORF220 copy number were male sterile
The key has been kept as simple as possible so it can be used by both the experienced and inexperienced. We have attempted to keep it non-technical and, on the whole have tried to score genera as a non-expert would (flora features like the presence of a perianth are an exception). The key uses mainly non-flower and fruit characters, so that it can be used to identify sterile plants, as these are the hardest to identify for expert and non-expert alike. In some cases, the key will not allow you to reduce the list to a single genus, but will take you to a group of related and very similar genera. Once you have a list of half a dozen genera, the images should allow you to identify a genus in most cases.. ...
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Gatan Microscopy Suite® (GMS) 是业界标准的(扫描)透射电子显微镜系统控制和结果分析软件。GMS 3 经过全面改造,现采用全新、简化的用户界面。GMS 3 可让新用户轻松实验基础的研究应用,同时还能继续为经验丰富的显微镜工作者提供其需要的深度访问和控制权限。 GMS 软件的卓越优势:
Renovation, Restoration, Restraint. I have just seen another 100 year old tiled floor and a panel of beautiful wall tiles (reminiscent of those in Monets kitchen at Giverny) hacked out and thrown in a skip. I understand all too well that initial urge to update, uprate & improve when presented with an old French house…
قارچ ها به دلیل رسوب نقره احیا شده در دیواره سلولی، به رنگ سیاه قهوه ای دیده می شوند.(زمینه به رنگ سبز کم رنگ دیده می شود.) نسبت به رنگ پاس کنتراست بهتری ب
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The present investigation was undertaken with a view of expedition of putative restorers for the development of high yielding rabi sorghum hybrids. Three CMS line were tested with ten restorers in line × tester mating design to evaluate seed set per cent and fertility restoration behavior. Restorers AKRB-335-3, Rb-413-1, AKRB-428, AKRB-429, AKRB-430 and AKRB-431 exhibited high fertility restoration ability in the CMS lines and therefore, hybrids viz. AKMS 30A×AKRB-335-3, AKMS 30A×AKRB-428, AKMS 30A×Rb-413-1, AKMS 30A×AKRB-429, AKMS 30A×AKRB-430, AKMS 30A×AKRB-431, AKRMS 45A×AKRB-335-3, AKRMS 45A×AKRB-428 and AKRMS 45A×Rb-413-1 showed high seed set percentage with anther fertility rating (9.0). The inheritance of fertility restoration revealed that observed frequency fits well into Mendelian ration of 3:1 for seed setting indicates that fertility restoration is governed by single dominant gene. Cluster analysis differentiate thirty hybrids in three classes, fully fertile revealed that ...
Cytoplasmic male sterility (CMS) has been exploited in the commercial pigeonpea [Cajanus cajan (L.) Millsp.] hybrid breeding system; however, the molecular mechanism behind this system is unknown. To understand the underlying molecular mechanism involved in A4 CMS system derived from C. cajanifolius (Haines) Maesen, 34 mitochondrial genes were analyzed for expression profiling and structural variation analysis between CMS line (ICRISAT Pigeonpea A line, ICPA 2039) and its cognate maintainer (ICPB 2039). Expression profiling of 34 mitochondrial genes revealed nine genes with significant fold differential gene expression at P ≤ 0.01, including one gene, nad4L, with 1366-fold higher expression in CMS line as compared with the maintainer. Structural variation analysis of these mitochondrial genes identified length variation between ICPA 2039 and ICPB 2039 for nad7a (subunit of nad7 gene). Sanger sequencing of nad4L and nad7a genes in the CMS and the maintainer lines identified two single ...
As a crop, flax holds significant commercial value for its omega-3 rich oilseeds and stem fibres. Canada is the largest producer of linseed but there exists scope for significant yield improvements. Implementation of mechanisms such as male sterility can permit the development of hybrids to assist in achieving this goal. Temperature sensitive male sterility has been reported in flax but the leakiness of this system in field conditions limits the production of quality hybrid seeds. Here, we characterized a 2,588 bp transcript differentially expressed in male sterile lines of flax. The twelve intron gene predicted to encode a 368 amino acid protein has five WD40 repeats which, in silico, form a propeller structure with putative nucleic acid and histone binding capabilities. The LuWD40-1 protein localized to the nucleus and its expression increased during the transition and continued through the vegetative stages (seed, etiolated seedling, stem) while the transcript levels declined during ...
San Juan Bautista CA (PRWEB) April 02 2013 Ag-Biotech Inc. a leading commercial plant genomics testing laboratory now offers DNA-based markers that can rapidly determine the cytoplasm [normal (N) male-fertile versus male-sterile (S)] and genotypes at the nuclear ma,Ag-Biotech,Offers,Male,Sterility,Locus,Marker,Services,for,Breeding,Onions,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
RNA editing of mitochondrial gene transcripts plays a central role during plant development and evolutionary adaptation. RNA editing has previously been reported to differ between the rice cytoplasmic male sterile (CMS) line and its maintainer line, which has been suggested as a cause for their different performances under environmental stress. To specifically test this hypothesis, a wild abortive (WA) CMS line (Huhan-1A) and its maintainer line (Huhan-1B) were utilized to investigate performances in response to oxidative stress, as well as RNA editing efficiencies on transcripts of six selected mitochondrial genes. Compared to the maintainer line, Huhan-1A represented both lower plant height and total antioxidant capacity, possessed higher total soluble protein and chlorophyll contents, accumulated less H2O2 content on the 3rd day after treatment (DAT), and exhibited higher survival ratio after re-watering. Furthermore, a total of 90 editing sites were detected on transcripts of six mitochondrial genes
Genetic variation exists in Raphanus that could be of use to Brassica breeders. Of particular interest is the Ogura system of cytoplasmic male sterility (CMS) which has been worked on extensively in a Brassica napus background. Problems have been experienced in B. napusrestorer lines due to the inheritance of a large segment of Raphanus chromosome containing the fertility restoring locus. This restorer introgression is located on the Brassica C genome making it only of use for B. napus and not for B. rapa or B. juncea. This thesis describes the development of the materials necessary for the introgression into the Brassica A genome of a defined segment of Raphanus chromosome containing a restorer locus. Defined genetic stocks of Raphanus were developed that contained specific loci controlling restoration of Ogura CMS. This material was used to develop populations segregating for specific restorer loci. Extensive RFLP maps of three Raphanus populations were developed and aligned, resulting in a ...
In higher plants, timely degradation of tapetal cells, the innermost sporophytic cells of the anther wall layer, is a prerequisite for the development of viable pollen grains. However, relatively little is known about the mechanism underlying programmed tapetal cell development and degradation. Here, we report a key regulator in monocot rice (Oryza sativa), PERSISTANT TAPETAL CELL1 (PTC1), which controls programmed tapetal development and functional pollen formation. The evolutionary significance of PTC1 was revealed by partial genetic complementation of the homologous mutation MALE STERILITY1 (MS1) in the dicot Arabidopsis (Arabidopsis thaliana). PTC1 encodes a PHD-finger (for plant homeodomain) protein, which is expressed specifically in tapetal cells and microspores during anther development in stages 8 and 9, when the wild-type tapetal cells initiate a typical apoptosis-like cell death. Even though ptc1 mutants show phenotypic similarity to ms1 in a lack of tapetal DNA fragmentation, delayed ...
The I3 cDNA isolated from a library made from 2-4 mm (immature) anthers of Brassica napus shows microspore-specific expression. Homologous transcripts are detected in buds and anthers of male-fertile plants, but not in green tissues, roots, or in cytoplasmic male-sterile buds. High expression of the transcript is limited to microspores entering and undergoing mitosis. The predicted peptide sequence of the cDNA shows an unusual repeated alanine/proline motif at the C-terminus, which may be of importance in the native protein structure.. ...
MAIS (PFLANZENBAU); MÄNNLICHE STERILITÄT VON KULTURPFLANZEN (PFLANZENZÜCHTUNG); ERTRAGSPHYSIOLOGIE (KULTURPFLANZEN); MAIZE (CROP PRODUCTION); MALE STERILITY OF CULTIVATED PLANTS (PLANT BREEDING); CROP YIELD PHYSIOLOGY (CROP PLANTS ...
Reagents and test methods for rapidly and specifically testing maize plants for the presence of T-type cms are provided. The reagent includes a novel nucleic acid segment whose sequence is uniquely arranged in mitochondrial DNA of cms-T maize. The segment, designated TURF 2H3, was cloned and vectors comprising TURF 2H3 provided. Subclones having sequences specific to cms-T mitochondrial DNA and the DNA and deduced amino acid sequences of ORI3, which is unique to T-type cytoplasm, are also provided.
Read more about Hybrid seeds new growth area in agro-chemicals on Business Standard. Companies aim breeding new varieties of self-immunity seeds to reduce uses of agrochemicals
In a post on Google+, Jean-Baptiste Quéru, long-time maintainer of the Android Open Source Project, has said hell no longer be working on it. Theres no point being the maintainer of an Operating System that cant boot to the home screen on its flagship device for lack of GPU support, especia...
When a new package maintainer joins the Fedora Project, we request that he/she introduces themselves on the Fedora devel mailing list. To sign up for the list, visit the devel lists signup page. The primary purpose of this is to begin the process of building trust by allowing the Fedora community members to get to know you a bit more and increase the chances of your review request being processed sooner. We want to break anonymity and foster real-world community within the project. You are under no obligation to reveal personal secrets. The objective is to establish a level of trust with yourself and the other members of the project. But you should at least use your real name when communicating with us. Also a brief description of who you are, your motivations and perhaps a description of the software you have submitted for review is advisable. ...
Living Maintenance - Maintainers Winning the Battle of the Bulge - Originally Posted by silverbirch Ive just come in from my Italian class and had a
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A commit references this bug: Author: jbeich Date: Thu Jan 18 00:19:05 UTC 2018 New revision: 459300 URL: https://svnweb.freebsd.org/changeset/ports/459300 Log: audio/hydrogen: update to 0.9.7 - Track direct dependencies on qt4-core - Convert to _CMAKE_BOOL option helper - Lift BROKEN_powerpc64 after USES=compiler:c++11-lang Changes: http://www.hydrogen-music.org/hcms/node/17207 PR: 225285 Submitted by: Shane Ambler (maintainer) Changes: head/audio/hydrogen/Makefile head/audio/hydrogen/distinfo head/audio/hydrogen/files/patch-CMakeLists.txt head/audio/hydrogen/files/patch-cmake_FindHelper.cmake head/audio/hydrogen/pkg- ...
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Mixing different RPM repositories that were not designed to be mixed can lead to incompatibilities that often result in dependency resolution problems in yum or up2date. Sometimes it even happens that software is not working as expected if libraries and applications come from different repositories. EPEL is designed as an add-on repository for RHEL and compatible derivatives. The best way to avoid problems is to avoid mixing EPEL with other third party repositories that have conflicting packages on the same system. Some people nevertheless do it and the yum priorities plugin can help to avoid the worst problems. If you encounter a problem where packages from EPEL are incompatible with another repository, or lead yum or up2date to bail out during dependency resolution, please report a bug to Bugzilla and contact the maintainer of the other repositories. The EPEL project encourages its maintainers to solve such problems together with the maintainers from other repositories in order to find a ...
Mosinger, Bedrich et al "Genetic loss or pharmacological blockade of testes-expressed taste genes causes male sterility." Proceedings of the National Academy of Sciences (2013): 201302827. Web. 11 Aug. 2020. ...
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On 6/9/2017 4:06 AM, Soegtrop, Michael wrote: , Dear Blake, , ,, External products were being lazy by relying on cygwin to strip CR when they ,, should have stripped it themselves. But sed -b does NOT strip CR (it is the ,, exact opposite, of keeping CR unstripped). , , I think that there are more people who use sed for text processing in a MinGW cygwin cross environment than there are people using sed for binary data - but looking at the mailing list this might be a subjective view. The maintainers of the Linux centric SW could insert dos2unix in all stuff piped into sed, but this would only be required in this very specific build configuration, and Linux SW maintainers frequently argue why they should care about Windows at all. It can take all sorts of philosophical discussions to get such a change in. And I cannot blame them for a we are free SW people, if you Windowers can make use of our SW without bothering us - go ahead, but please dont drag us into your mud attitude. In the end one ...
Did you ever need to insert a init.d scripts Y before init.d scripts Z and after init.d script X, where both Z and X is started using boot sequence number 20 (as in 20X and 20Z)? Adding your script as 20Y would not cut it, because you need Z to start before X. To do this, in Debian you currently need to talk to the maintainer of packages with scripts Z and X, asking one of them (or both) to move their script into a different number. Dependency based boot sequencing make it possible for each package maintainer to specify the dependencies of its scripts, and leave the number allocation to the dependency resolver. Some examples of such problems are 256699 294053, 279297, 311057 and 475626 . ...
Did you ever need to insert a init.d scripts Y before init.d scripts Z and after init.d script X, where both Z and X is started using boot sequence number 20 (as in 20X and 20Z)? Adding your script as 20Y would not cut it, because you need Z to start before X. To do this, in Debian you currently need to talk to the maintainer of packages with scripts Z and X, asking one of them (or both) to move their script into a different number. Dependency based boot sequencing make it possible for each package maintainer to specify the dependencies of its scripts, and leave the number allocation to the dependency resolver. Some examples of such problems are 294053, 279297 and 475626 . ...
The use of done_testing() in the newly-added POD coverage test is redundant because all_pod_coverage_ok() define its own plan. If you really need to use it, a better test for its availability is to check for Test::More version 0.88 or later rather than checking for perl 5.8.6 or later. In case youre wondering, I (as a downstream packager) habitually run maintainer tests, not because they add value for the module Im packaging at the time but to give wider testing for the maintainer test modules themselves ...
On Fri, Mar 11, 2016 at 04:16:58PM +0000, Alex Bennée wrote: , , Fam Zheng ,[email protected], writes: , , , v3 changes: , , I think we are almost there. There a just a few tweaks to be made to , help text and prompts. Can you ensure that all examples in commit , messages and help text actually do run as expected? , , Is it proposed this goes through Daniels treee? What tree is that youre referring to ? I certainly dont consider myself the maintainer of all tests in QEMU :-) Id say that Fam is maintainer of tests/docker/ and should thus just send PULL for it request(s) directly Regards, Daniel -- ,: http://berrange.com -o- http://www.flickr.com/photos/dberrange/ :, ,: http://libvirt.org -o- http://virt-manager.org :, ,: http://autobuild.org -o- http://search.cpan.org/~danberr/ :, ,: http://entangle-photo.org -o- http://live.gnome.org/gtk-vnc ...
In the case of features impacting individual activities, the person responsible is the activity maintainer. The list of maintainers is available on the activity page in [http://activities.sugarlabs.org, ...
What is the status of this package, please --- will it be fixed, For fixes, the package maintainer needs to be found and contacted, or a new maintainer (you? :) come forward. Per the thread from September 9 2013 that you mentioned. or deleted? In general, we dont delete packages from TL unless they are removed or marked obsolete on CTAN. That would be an unusual thing to do in this case. best, karl ...