Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.. In our series of projects for accumulating sequence information on the coding sequences of unidentified human genes, we have newly determined the sequences of 100 cDNA clones from a set of size-fractionated human brain cDNA libraries, and predicted the coding sequences of the corresponding genes, named KIAA0711 to KIAA0810. These cDNA clones were selected according to their coding potentials of large proteins (50 kDa and more) in vitro. The average sizes of the inserts and corresponding open reading frames were 4.3 kb and 2.6 kb (869 amino acid residues), respectively. Sequence analyses against the public databases indicated that the predicted coding sequences of 78 genes were similar to those of known genes, 64% of which (50 genes) were categorized as proteins functionally related to cell signaling/communication, cell structure/motility and nucleic acid management. As additional information ...
Nagase T، Ishikawa K، Nakajima D، Ohira M، Seki N، Miyajima N، Tanaka A، Kotani H، Nomura N، Ohara O (Apr 1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research. 4 (2): 141-50. PMID 9205841. doi:10.1093/dnares/4.2.141. ...
Ishikawa K، Nagase T، Suyama M، وآخرون. (1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.". DNA Res. 5 (3): 169-76. PMID 9734811. doi:10.1093/dnares/5.3.169. ...
1Dv1 : Version assembled with FPC (1D, 4D, 6D were generated as a single project). We used markers data to assign BAC on 1D chromosome. Unassigned data from this experiment are available here. ...
Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro ...
Build: Sat Nov 17 23:53:08 EST 2018 (commit: a759bb7). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
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Department of Genetics, Harvard Medical School, Boston, MA, USA.. A method for construction of bacterial artificial chromosome (BAC) contigs from a yeast artificial chromosome (YAC) physical map is described. An approximately 2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing.. MeSH Terms ...
Spermatogenesis-associated protein 2 is a protein that in humans is encoded by the SPATA2 gene. GRCh38: Ensembl release 89: ENSG00000158480 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000047030 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". "Entrez Gene: SPATA2 spermatogenesis associated 2". Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791-806. doi:10.1101/gr.6.9.791. PMID 8889548. Nagase T, Ishikawa K, Suyama M, et al. (1999). "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 5 (5): 277-86. doi:10.1093/dnares/5.5.277. PMID 9872452. Graziotto R, Foresta C, Scannapieco P, et al. (1999). "cDNA cloning and characterization of PD1: a novel human testicular protein with different expressions in various testiculopathies". Exp. Cell Res. 248 (2): ...
Texas public colleges and universities are in the process of implementing the states contentious new campus carry law, which come Aug. 1 will allow anyone with a "license to carry" to bring concealed handguns into most college buildings. Citing unique security concerns, several campuses have proposed designating certain areas as "gun-free" zones. The Dallas Morning News has surveyed each public campus - along with a few state medical schools, including all of those in the Dallas area - to see what progress theyve made in developing their policies. READ MORE ...
3 (fi 6) (either term, 3-0-3). The chromosomal and molecular basis for the transmission and function of genes. The construction of genetic and physical maps of genes and genomes. Strategies for the isolation of specific genes. Examples of regulatory mechanisms for the expression of the genetic material in both prokrayotes and eukaryotes. Note: Students may not obtain credit for both BIOL 207 and GENET 197. Prerequisite: BIOL 107.. ...
The development of a universal soybean (Glycine max [L.] Merr.) cytogenetic map that associates classical genetic linkage groups, molecular linkage groups, and a sequence-based physical map with the karyotype has been impeded due to the soybean chromosomes themselves, which are small and morphologically homogeneous. To overcome this obstacle, we screened soybean repetitive DNA to develop a cocktail of fluorescent in situ hybridization (FISH) probes that could differentially label mitotic chromosomes in root tip preparations. We used genetically anchored BAC clones both to identify individual chromosomes in metaphase spreads and to complete a FISH-based karyotyping cocktail that permitted simultaneous identification of all 20 chromosome pairs. We applied these karyotyping tools to wild soybean, G. soja Sieb. and Zucc., which represents a large gene pool of potentially agronomically valuable traits. These studies led to the identification and characterization of a reciprocal chromosome ...
ATAC002388 AC002388 85534bp DNA PLN 15-AUG-1998 Arabidopsis thaliana chromosome II BAC T13E15 genomic sequence, complete sequence. HTG; T13E15.1; T13E15.2; T13E15.3; T13E15.4; T13E15.5; TINY transcription factor isolog; T13E15.6; T13E15.7; T13E15.8; homeodomain-zipper protein, ATHB-4; T13E15.9; T13E15.10; cytochrome P450 isolog; T13E15.11; T13E15.12; T13E15.13; 60S ribosomal protein L30 isolog; T13E15.14; T13E15.15; EREBP isolog; T13E15.16; serine/threonine protein kinase isolog; T13E15.17; T13E15.18; triacyglycerol lipase isolog; T13E15.19; giberellin beta-hydroxylase isolog, 5 partial ...
Previous genetic studies had pointed to a region on mouse chromosome 1 as containing a gene responsible for BMD regulation. In the current project, Mohan and his colleagues honed in on this region of chromosome 1 using a variety of molecular techniques, and they located a gene called DARC (Duffy Antigen Receptor for Chemokines) that exhibited different levels of expression in mice with higher BMD. The analogous chromosomal region has also been shown to influence osteoporosis in humans ...
When have you last used a heart rate monitor?Only during certain workouts is it wise for ultra runners to track the numbers down.Find out when it most matters to use your heart rate monitor
Although second generation sequencing (2GS) technologies allow re-sequencing of previously gold-standard-sequenced genomes, whole genome shotgun sequencing and de novo assembly of large and complex eukaryotic genomes is still difficult. Availability of a genome-wide physical map is therefore still a prerequisite for whole genome sequencing for genomes like barley. To start such an endeavor, large insert genomic libraries, i.e. Bacterial Artificial Chromosome (BAC) libraries, which are unbiased and representing deep haploid genome coverage, need to be ready in place. Result: Five new BAC libraries were constructed for barley (Hordeum vulgare L.) cultivar Morex. These libraries were constructed in different cloning sites (HindIII, EcoRI, MboI and BstXI ) of the respective vectors. In order to enhance unbiased genome representation and to minimize the number of gaps between BAC contigs, which are often due to uneven distribution of restriction sites, a mechanically sheared library was also ...
Coordinates: 22°11′52″N 113°32′26″E / 22.19778°N 113.54056°E / 22.19778; 113.54056 The Na Tcha Temple (Portuguese: Templo Na Tcha; Chinese: 大三巴哪吒廟), built in 1888, is a Chinese folk religion temple in Santo António, Macau, China dedicated to the worship of the deity Na Tcha. The Na Tcha Temple was built in homage to the child god of war. It is believed that it was built to put an end to the plague ravaging the region during that time. The small traditional Chinese temple is a simple, single chamber building measuring 8.4 meters (28 ft) long and 4.51 meters (14.8 ft) wide. The entrance porch opens to the temple building measuring 5 meters (16 ft) in depth. The building is painted gray, with few ornamentations, except for paintings on walls under the entrance porch. The temples roof, rising five meters, is of the traditional yingshan style. True to traditional Chinese architecture, the Na Tcha has protective ceramic animal figures on its ridge. Na Tcha Temple is ...
Genome analysis of soybean (Glycine max L.) has been complicated by its paleo-autopolyploid nature and conserved homeologous regions. Landmarks of expressed sequence tags (ESTs) located within a minimum tile path (MTP) of contiguous (contig) bacterial artificial chromosome (BAC) clones or radiation hybrid set can identify stress and defense related gene rich regions in the genome. A physical map of about 2,800 contigs and MTPs of 8,064 BAC clones encompass the soybean genome. That genome is being sequenced by whole genome shotgun methods so that reliable estimates of gene family size and gene locations will provide a useful tool for finishing. The aims here were to develop methods to anchor plant defense- and stress-related gene paralogues on the MTP derived from the soybean physical map, to identify gene rich regions and to correlate those with QTL for disease resistance. The probes included 143 ESTs from a root library selected by subtractive hybridization from a multiply disease resistant soybean
We have built a robust rice physical map. More than 65,000 BAC clones representing 20-fold coverage have been fingerprinted successfully and assembled into physical contigs. The integrity of the contig assembly and clone order has been confirmed independently by FPC Simulated Digest using sequenced BAC and PAC clones from GenBank. Approximately 90% of the rice genome has been anchored genetically. Among the genetically anchored contigs, ∼80% are anchored by two or more genetic markers and therefore are oriented properly, whereas ,80% are anchored by multiple methods (i.e., marker hybridization, in silico hybridization, FISH, and sequenced clones).. On the basis of the physical map, we estimated the euchromatic portion of the rice genome to be ∼400 Mb, whereas earlier studies estimated the rice genome to be 430 Mb, based on DNA content (Arumuganathan and Earle, 1991; Saji et al., 2001). In contrast to the previous estimate of 51.5 Mb for chromosome 1 (Table 1) (Saji et al., 2001), our size ...
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Пример добавления картинок в ms word. Точно работает с 2007, но по идее должен работать и с остальными постарше. Берёт кучу картинок из каталога с картинками, добавляет в документ и сохраняет в этот же каталог с именем каталога. Lazarus 2.0 + FPC 3.0. ...
Author Summary During growth and development, all plants and animals must replicate their DNA. This process is regulated to ensure that all sequences are completely and accurately replicated and is limited to S phase of the cell cycle. In the cell, DNA is packaged with histone proteins into chromatin, and both DNA and histones are subject to epigenetic modifications that affect chromatin state. Euchromatin and heterochromatin are chromatin states marked by epigenetic modifications specifying open and closed conformations, respectively. Using the model plant Arabidopsis thaliana, we show that the time at which a DNA sequence replicates is influenced by the epigenetic modifications to the surrounding chromatin. DNA replication occurs in two phases, with euchromatin replicating in early and mid S phase and heterochromatin replicating late. DNA replication time has been linked to gene expression in other organisms, and this is also true in Arabidopsis because more genes are active in euchromatin when
Our initial mapping data placed Mcd4 into two possible locations. In both cases, it was desirable to generate bacterial artificial chromosome (BAC) contigs for eventual complementation, as well as additional markers. As a case study, we describe how BAC contigs can be extended using Chlamydomonas resources and our experience in generating site-specific markers.. Several BAC libraries have been constructed for Chlamydomonas, two of which are available through the Clemson Genomics Institute (http://www.genome.clemson.edu/groups/bac/). In addition, BAC contigs (http://www.biology.duke.edu/chlamy_genome/BAC/index.html) have been assembled for most of the STS and RFLP markers. In the case of mcd4, Gsp1 resides on scaffold 2 and CNA45 on scaffold 66. A 41-BAC contig exists for scaffold 2 covering approximately 1,000 kb (R. Nguyen, personal communication), and a smaller contig is linked to scaffold 66 and CNA45 (Fig. 6). An unknown amount of DNA separates scaffolds 2 and 66; such discontinuities in the ...
Distribution of contigs by size of longest ORF. Solid line, contigs with any database homology by BLASTX (1,445). Dotted line, contigs without database homology
Title: Physical map construction and physical characterization of channel catfish genome. Name: Xu, Peng. Degree: PhD. Chair: Zhanjiang Liu. Resides: FAA Library. University: Auburn. Location: Auburn, Alabama. Date: 2007. Pages: 111. Keywords: channel catfish genome, gene mapping, genetics. Abstract: Catfish is the major aquaculture species in the United States. To enhance genome studies involving linkage mapping, comparative mapping and linkage map and physical map integration, over 20,000 Bacterial Artificial Chromosome (BAC) end sequences were generated and a BAC-based physical map of the channel catfish (Ictalurus punctatus Rafinesque) genome was constructed using four color fluorescence-based fingerprinting. A total of 25,195 BAC ends were sequenced, generating 20,366 clean BAC end sequences (BES) with an average reading length of 557 bp. The total reading length of 11,414,601 bp represented approximately 1.2% of the catfish genome. Based on this survey, the catfish genome was found to be ...
LOCUS ATU53502 11862 bp DNA PLN 05-MAY-1996 DEFINITION Arabidopsis thaliana chromosome I cosmid g17311 DNA. ACCESSION U53502 KEYWORDS . SOURCE thale cress strain=Columbia. ORGANISM Arabidopsis thaliana Eukaryotae; mitochondrial eukaryotes; Viridiplantae; Charophyta/Embryophyta group; Embryophyta; Magnoliophyta; Magnoliopsida; Capparales; Brassicaceae; Arabidopsis. REFERENCE 1 (bases 1 to 11862) AUTHORS Goodman,H.M. and Gallant,P. TITLE Sequence of cosmid g17311 from Arabidopsis thaliana chromosome I JOURNAL Unpublished REFERENCE 2 (bases 1 to 11862) AUTHORS Goodman,H.M. and Gallant,P. TITLE Direct Submission JOURNAL Submitted (03-APR-1996) John Morris, Molecular Biology, Massachusetts General Hospital / Harvard Medical School, 50 Blossom St., Boston, MA 02114, USA FEATURES Location/Qualifiers source 1..11862 /organism="Arabidopsis thaliana" /strain="Columbia" /chromosome="I" /map="1; between m132 and mi157 at 159 cM" misc_feature 1..7505 /note="sequence overlap with cosmid g8261" misc_feature ...
As part of the effort to sequence the genome of Rattus norvegicus, we constructed a physical map comprised of fingerprinted bacterial artificial chromosome (BAC) clones from the CHORI-230 BAC library. These BAC clones provide approximately 13-fold redundant coverage of the genome and have been assembled into 376 fingerprint contigs. A yeast artificial chromosome (YAC) map was also constructed and aligned with the BAC map via fingerprinted BAC and P1 artificial chromosome clones (PACs) sharing interspersed repetitive sequence markers with the YAC-based physical map. We have annotated 95% of the fingerprint map clones in contigs with coordinates on the version 3.1 rat genome sequence assembly, using BAC-end sequences and in silico mapping methods. These coordinates have allowed anchoring 358 of the 376 fingerprint map contigs onto the sequence assembly. Of these, 324 contigs are anchored to rat genome sequences localized to chromosomes, and 34 contigs are anchored to unlocalized portions of the ...
8-base recognition sites will yield pieces 64,000 bases long. Since hundreds of different restriction enzymes have been characterized, DNA can be cut into many different small fragments. Physical Maps Different types of physical maps vary in their degree of resolution. The lowest- resolution physical map is the chromosomal (sometimes called cytogenetic) map, which is based on the distinctive banding patterns observed by light microscopy of stained chromosomes. A cDNA map shows the locations of expressed DNA regions (exons) on the chromosomal map. The more detailed cosmid contig map depicts the order of overlapping DNA fragments spanning the genome. A macrorestriction map describes the order and distance between enzyme cutting (cleavage) sites. The highest- resolution physical map is the complete elucidation of the DNA base- pair sequence of each chromosome in the human genome. Physical maps are described in greater detail below. Low-Resolution Physical Mapping Chromosomal map. In a chromosomal ...
This page provides quick access to the Comparative mapping functions available in the Map Viewer. Currently, comparative maps are calculated using HomoloGene orthology predictions. Once the gene pairs have been established, blocks of conserved syteny can be established using the positions of each gene object in their respective builds. Data used to calculate the Gene Table View is available from our FTP site.. ...
Illustration of integration of the linkage map (left) with the physical map (right). The linkage group 12 (LG12) was used for the illustration. Vertical bars re
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Quint, M., Mihaljevic, R., Dussle, C.M., Xu, M.L., Melchinger, A.E. & Lübberstedt, T. Development of RGA-CAPS markers and genetic mapping of candidate genes for SCMV resistance in maize Theor Appl Genet 105, 355-366, (2002) ...
大野 京子 , 丹羽 愛樹 , 加藤 志津香 , 近藤 恭司 , 織田 銑一 , 井上 稔 , 山村 英樹 Experimental Animals 43(2), 251-255, 1994 J-STAGE 医中誌Web 被引用文献3件 ...
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Heres something all the means girls can write about in their notebooks: Rachel McAdams is now a redhead.The Vow star ventured to Disneyland Wednesday with a new do, according to US Magazine.Rachel McAdams and Michael Sheen splitMcAdams, 34, kept her ...
Genome sequencing of barley has been delayed due to its large genome size (ca. 5,000Mbp). Among the fast sequencing systems, 454 liquid phase pyrosequencing provides the longest reads and is the most promising method for BAC clones. Here we report the results of pooled sequencing of BAC clones selected with ESTs genetically mapped to chromosome 3H. We sequenced pooled barley BAC clones using a 454 parallel genome sequencer. A PCR screening system based on primer sets derived from genetically mapped ESTs on chromosome 3H was used for clone selection in a BAC library developed from cultivar Haruna Nijo. The DNA samples of 10 or 20 BAC clones were pooled and used for shotgun library development. The homology between contig sequences generated in each pooled library and mapped EST sequences was studied. The number of contigs assigned on chromosome 3H was 372. Their lengths ranged from 1,230 bp to 58,322 bp with an average 14,891 bp. Of these contigs, 240 showed homology and colinearity with the genome
OPPORTUNITY TO PROPOSE ORGANISMS FOR BAC LIBRARY CONSTRUCTION Release Date: December 19, 2001 NOTICE: NOT-HG-02-004 National Human Genome Research Institute Annual Submission Dates: February 10, June 10 and October 10 Over the past several years, the bacterial artificial chromosome (BAC) has emerged as the vector system of choice for the construction of the large- insert chromosomal DNA libraries that are needed in genomic studies. Because BAC clones are relatively large and appear to faithfully represent an organisms genome, the BAC system will also be the vehicle of choice for the isolation of targeted regions of genomic DNA from additional organisms being used in specific biological studies, a variety of mouse strains, and even from individual humans. With the increasing interest in genomic approaches to biological research, the demand for new BAC libraries is expected to increase rapidly in the next several years. To meet the need to increase the number of available BAC libraries, NHGRI, ...
Segmental structure of various scales exists in genomic sequences. Many evolutionary and genetic mechanisms leading to variation in DNA operate on segments of the genome (duplications and inversions of segments, recombinations). Furthermore, eukaryotic chromosomes consist of alternating regions of gene-rich and gene-poor regions. A gene-rich region can be further decomposed into non-coding segments, segments that contain regulatory information, and genes, which in turn consist of introns and exons. Also, remnants of viral or microbial inserts in a genome form a type of segmental structure.. There are many types of features with which one can segment the sequences. For any given technique, there may exist alternative biological features to segment on. For example, if the goal is to identify coding and noncoding segments in a sequence, one may study the distribution of three-letter words (codons) along the sequence to determine where to set the segment boundaries. An alternative would be, for ...
Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces ...
利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Genes Genet. Syst. 83, 245-256, 2008)。ショウジョウバエBACクローンを用いた研究成果の公表に当たっては、ナショナルバイオリソースプロジェクト「ショウジョウバエ」に対する謝辞の表明を必要とする ...
SCORE============================================Contig000140============================================ 668 ============================================Contig002535============================================ 810 2 -----------------------------------------------------------------------------------,JMFF026E19 ,----------------------------------------------------------------------------------------------rJMFF026E19 2 --------------------------------------------------------------------------------------------,JMFF035C08 ,----------------------------------------------------------------------------------------------rJMFF035C08 4 -----------------------------------------------------------------------------------------,JMFF009N06 ,----------------------------------------------------------------------------------------------rJMFF009N06 9 -------------------------------------------------------------------,JMFF012H01 ...
SCORE============================================Contig004682============================================ 660 ============================================Contig013845============================================1162 57 --------------------------------------------------------------------------------,JMFF051H21 ,----------------------------------------rJMFF051H21 68 -----------------------------------------------------------------------------------------------,JMFF044I04 ,----------------------------------------------------rJMFF044I04 72 ---------------------------------------------------------------------,JMFF037P06 ,-----------------------------------------------------------------rJMFF037P06 81 ------------------------------------------------------------------------------------------,JMFF025B05 ,-----------------------------------------------------------------rJMFF025B05 110 -----------------------------------------------------------------------------,JMFF028E21 ...
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Looking for online definition of chromosome walking in the Medical Dictionary? chromosome walking explanation free. What is chromosome walking? Meaning of chromosome walking medical term. What does chromosome walking mean?
To align the developing chicken BAC contig physical maps with the existing linkage map, its necessary to identify BACs corresponding to the DNA-based markers on the latter. Chicken BAC libraries, derived from DNA of a single UCD001 inbred Red Jungle Fowl, have been generated by our collaborators. Characterization of the first BAC library based on BamHI partial digest fragments initially was done by filter hybridization with pools of labeled, PCR-amplified fragments based on marker or gene DNA sequences. Individual marker/BAC assignments were made by Southern hybridization of BAC DNA with individual marker probes and/or PCR analysis. In this manner, 31 markers from 9 linkage groups generated 71 BamHI BAC candidates (2.3 clones per locus). This approach is labor- and cost-intensive. Thus, we began using pools of overgo probes, that are complementary synthetic oligonucleotides extended in vitro to generate ~40 base pair, double-stranded DNA probes. Two BAC libraries were hybridized to pools of 36 ...
Read "A comparative linkage and physical map of bovine chromosome 24 with human chromosome 18, Mammalian Genome" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
To increase the utility of the previously constructed physical map of the chromosome of Neisseria gonorrhoeae FA1090, 28 additional genetic markers were localized on the map. Cloned gonococcal genes were used to probe Southern blots of restriction enzyme-digested DNA separated on pulsed-field gels, thus identifying the fragment in each of several digests to which the probe hybridized and the map location of each gene. The addition of the new markers brings the total number of mapped loci for this strain to 68; the locations of all of those markers on the updated map are shown. ...
We have cloned a gene, ZFF29 (zinc-finger protein of human fetal liver erythroid cells 29), from human fetal liver erythroid cells. Two types of mature mRNA were identified and designated ZFF29a and ZFF29b. In human genome the ZFF29 gene is on chromosome 9q, and the two forms are splice variants. There is a unique transcription start site, which predicts major mRNAs composed of 2485 bases for ZFF29a and 1801 bases for ZFF29b. The anticipated mRNAs were demonstrated in K562 cells, but not in any adult human tissues examined by Northern blotting. In the mouse, reverse transcription-PCR revealed that the ZFF29 mRNA is present in adult bone marrow and ovary at a higher level than in any other tissues examined. These findings suggest that ZFF29 proteins are expressed in embryonic/fetal erythroid tissues. The deduced polypeptide chains of ZFF29a and ZFF29b are composed of 306 and 350 amino acids respectively. A unique zinc-finger motif composed of two contiguous Cys2His2-type fingers is common to both ...