selenophosphate synthetase: involved in selenium metabolism; gamma-phosphate of ATP is transferred to HSe resulting in formation of monoselenophosphate; amino acid sequence in first source
SEPHS2 antibody (selenophosphate synthetase 2) for ICC/IF, IHC-P, WB. Anti-SEPHS2 pAb (GTX103766) is tested in Human samples. 100% Ab-Assurance.
phospho-N-acetylmuramoyl pentapeptide transferase: enzyme important in murein synthesis which transfers the N-acetylmuramoylpentapeptide to a membrane-bound carrier that is a C-55 terpene alcohol phosphate; minor descriptor (75-84); on-line & Index Medicus search PHOSPHOTRANSFERASES (75-84)
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Several hits in gapped BLAST to uridine kinase sequences, e.g. residues 4-206 are 41% similar to the enzyme from B.subtilis (Z99117). UU332 is similar to MG382 and, weakly, to TP0667, two predicted uridine kinases. No similarity to C.trachomatis ...
Kinase molecule, computer model. Kinases, also known as phosphotransferases, are enzymes that catalyse the transfer of phosphate groups from a high-energy phosphate-containing molecule (such as ATP or ADP) to a substrate (such as a protein, lipid or carbohydrate). This process is known as phosphorylation. The largest group of kinases are protein kinases. Up to 518 different types have been identified in humans and are used to transmit signals and control complex processes in cells. In this model the molecules shape (secondary structure) is seen, comprising alpha helices (coils), beta sheets (arrows) and linking regions (lines). - Stock Image A605/0207
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor.
Glycerol uptake and glycerol kinase activity were studied in primary cultures of rat hepatocytes in the presence of either 1 nM insulin, 1 nM glucagon, or 100 nM dexamethasone, alone or in combination in
Thus, the two substrates of this enzyme are ATP and uridine, whereas its two products are ADP and UMP. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:uridine 5-phosphotransferase. Other names in common use include pyrimidine ribonucleoside kinase, uridine-cytidine kinase, uridine kinase (phosphorylating), and uridine phosphokinase. This enzyme participates in pyrimidine metabolism. ...
... , Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
The IUPHAR/BPS Guide to Pharmacology. phosphoinositide-3-kinase regulatory subunit 1 - Phosphatidylinositol kinases. Detailed annotation on the structure, function, physiology, pharmacology and clinical relevance of drug targets.
Journal of Amino Acids is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of amino acids.
... , Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
Ratliff, R.L., Weaver, R.H., Lardy, H.A. and Kuby, S.A. (1964). "Nucleoside triphosphate-nucleoside diphosphate transphosphorylase (nucleoside diphosphokinase). I. Isolation of the crystalline enzyme from brewers yeast". J. Biol. Chem. 239: 301-309. PMID 14114857. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Saint Girons, I.S., Gilles, A.-M., Margarita, D., Michelson, S., Monnot, M., Fermandjian, S., Danchin, A. and Barzu, O. (1987). „Structural and catalytic characteristics of Escherichia coli adenylate kinase". J. Biol. Chem. 262: 622-629. PMID 3027060 ...
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found o
Adenylate Kinase 1兔多克隆抗体(ab71621)可与仓鼠, 人样本反应并经WB, ELISA, IHC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
MTSQEKTEEYPFADIFDEDETERNFLLSKPVCFVVFGKPGVGKTTLARYITQAWKCIRVEALPILEEQIA 1 - 70 AETESGVMLQSMLISGQSIPDELVIKLMLEKLNSPEVCHFGYIITEIPSLSQDAMTTLQQIELIKNLNLK 71 - 140 PDVIINIKCPDYDLCQRISGQRQHNNTGYIYSRDQWDPEVIENHRKKKKEAQKDGKGEEEEEEEEQEEEE 141 - 210 AFIAEMQMVAEILHHLVQRPEDYLENVENIVKLYKETILQTLEEVMAEHNPQYLIELNGNKPAEELFMIV 211 - 280 MDRLKYLNLKRAAILTKLQGAEEEINDTMENDELFRTLASYKLIAPRYRWQRSKWGRTCPVNLKDGNIYS 281 - 350 GLPDYSVSFLGKIYCLSSEEALKPFLLNPRPYLLPPMPGPPCKVFILGPQYSGKTTLCNMLAENYKGKVV 351 - 420 DYAQLVQPRFDKARETLVENTIAEATAAAIKVVKEKLLRELQARKQAETALREFQRQYEKMEFGVFPMEA 421 - 490 THSSIDEEGYIQGSQRDRGSSLVDTEEAKTKSENVLHDQAAKVDKDDGKETGETFTFKRHSQDASQDVKL 491 - 560 YSDTAPTEDLIEEVTADHPEVVTMIEETIKMSQDINFEQPYEKHAEILQEVLGEVMEENKDRFPGAPKYG 561 - 630 GWIVDNCPIVKELWMALIKKGIIPDLVIYLSDTENNGKCLFNRIYLQKKSEIDSKILERLLEELQKKKKE 631 - 700 EEEARKATEEELRLEEENRRLLELMKVKAKEAEETDNEDEEEIEGDELEVHEEPEASHDTRGSWLPEEFE 701 - 770 ASEVPETEPEAVSEPIEETTVETEIPKGSKEGLEIEKLSETVVLPEFPEDSYPDVPEMEPFKEKIGSFII 771 - 840 ...
myokinase definition: adenylate kinase, a phosphotransferase chemical that catalyzes the interconversion of adenine nucleotides and plays a crucial role in mobile energy homeostasis.
Considerable differences in steady-state hexokinase specific activity were found in 16 N.C.I.B. strains of Klebsiella aerogenes grown in identical conditions in glucose-limited chemostats. Strains of N.C.I.B. 8258 had no detectable activity, but its glucose-phosphoenolpyruvate phosphotransferase specific activity and that of the other strains were closely similar, and it is concluded that this phosphotransferase activity regulates the overall utilization of glucose, in which hexokinase plays no essential role. The hexokinase activity was subject to regulation by the availability of phosphorus, but this did not affect the glucose phosphotransferase activity. tlactose-grown organisms (including strain N.C.I.B. 8258) had no glucose phosphotransferase activity, but more than adequate hexokinase activity to phosphorylate the intracellularly liberated glucose. ...
Definition of Phosphoglyceromutase with photos and pictures, translations, sample usage, and additional links for more information.
Overview Protocols Specifications Resources Convenient, Scalable Kinase Profiling The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The TGF?R2 Kinase Enzyme System contains: TGF?R2 Kinase, 10ug (Human, recombinan
Overview Protocols Specifications Resources Convenient, Scalable Kinase Profiling The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The p38? Kinase Enzyme System contains: p38? Kinase, 10ug (Human, recombinant fu
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the ...
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the ...
Choline kinase (also known as CK,ChoK and choline phosphokinase) is an enzyme which catalyzes the first reaction in the choline pathway for phosphatidylcholine (PC) biosynthesis. This reaction involves the transfer of a phosphate group from adenosine triphosphate (ATP) to choline in order to form phosphocholine. ATP + choline ⇌ {\displaystyle \rightleftharpoons } ADP + O-phosphocholine Thus, the two substrates of this enzyme are ATP and choline, whereas its two products are adenosine diphosphate (ADP) and O-phosphocholine. Choline kinase requires magnesium ions (+2) as a cofactor for this reaction. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The first detailed investigation of the enzyme was conducted by McCamen in 1962, where it was shown that the brain is the richest source of the enzyme in mammalian tissue. A related enzyme, ethanolamine kinase, tends to co-purify ...
Order Adenylate Kinase 1 ELISA Kits for many Reactivities. Mouse and more. Compare Adenylate Kinase 1 ELISA Kits and find the right product on antibodies-online.com.
The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq, Jul 2008 ...
Shop Lichenan-specific phosphotransferase enzyme IIA component ELISA Kit, Recombinant Protein and Lichenan-specific phosphotransferase enzyme IIA component Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Fingerprint Dive into the research topics of A Mitochondrial RNAi Screen Defines Cellular Bioenergetic Determinants and Identifies an Adenylate Kinase as a Key Regulator of ATP Levels. Together they form a unique fingerprint. ...
1AKY: High-resolution structures of adenylate kinase from yeast ligated with inhibitor Ap5A, showing the pathway of phosphoryl transfer.
Activation and aggregation of blood platelets is crucial for hemostasis and thrombosis. In the vascular system adenine nucleotides are important signaling molecules playing a key role in hemostasis. ADP was the first low molecular weight agent recognized to cause blood platelets activation and aggregation. NTPDases and adenylate kinase (AK) are the main enzymes involved in metabolism of extracellular adenine nucleotides. The majority of studies concentrated on the role of NTPDase1 (apyrase) in the inhibition of platelets aggregation. Up to now, there are still insufficient data concerning the role of AK in this process. We found that adenylate kinase activity in the serum of patients with myocardial infarction is significantly increased when compared to the healthy volunteers. The elevated activity of AK is connected to appearance of another isoform of that enzyme, expressed in patients with myocardial infarction. The influence of AK on the pig blood platelets aggregation induced by 20 μM ADP or 7.5
The energy expenditure of another ATP in this step is justified in 2 ways: The glycolytic process (up to this step) is now irreversible, and the energy supplied destabilizes the molecule. Because the reaction catalyzed by Phosphofructokinase 1 (PFK-1) is coupled to the hydrolysis of ATP, an energetically favorable step, it is, in essence, irreversible, and a different pathway must be used to do the reverse conversion during gluconeogenesis. This makes the reaction a key regulatory point (see below). This is also the rate-limiting step. Furthermore, the second phosphorylation event is necessary to allow the formation of two charged groups (rather than only one) in the subsequent step of glycolysis, ensuring the prevention of free diffusion of substrates out of the cell. The same reaction can also be catalyzed by pyrophosphate-dependent phosphofructokinase (PFP or PPi-PFK), which is found in most plants, some bacteria, archea, and protists, but not in animals. This enzyme uses pyrophosphate (PPi) ...
The main aim of the work presented in this thesis was to elucidate the apparent role of pyrophosphate fructose 6-phosphate 1-phosphotransferase (PFP) in sucrose accumulation in sugarcane. PFP activity in sugarcane internodal tissue is inversely correlated to the sucrose content and positively to the water-insoluble component across varieties which differ in their capacities to accumulate sucrose. This apparent well defined and important role of PFP seems to stand in contrast to the ambiguity regarding PFPs role in the general literature as well as the results of various transgenic studies where neither the downregulation nor the over-expression of PFP activity had a major influence on the phenotype of transgenic potato and tobacco plants. Based on this it was therefore thought that either the kinetic properties of sugarcane PFP is significantly different than that of other plant PFPs or that PFPs role in sucrose accumulating tissues is different from that in starch accumulating tissues. In the ...
The two-component system is involved in several developmental events and different responses to the environment. Histidine-containing phosphotransfer prote
AimsTo measure the activity of the key phosphotransfer enzymes creatine kinase (CK), adenylate kinase (AK), and glycolytic enzymes in two common mouse models of chronic heart failure.Methods and resultsC57BL/6 mice were subjected to transverse aortic constriction (TAC), myocardial infarction induced
1BIF: The crystal structure of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies.
Kit Component:- KN302227G1, Bpgm gRNA vector 1 in pCas-Guide vector- KN302227G2, Bpgm gRNA vector 2 in pCas-Guide vector- KN302227D, donor vector…
Numerous significant hits using gapped BLAST to aspartokinase III from E. coli (416597), Arabidopsis thaliana (6091740, putative), Methanococcus jannaschii (2492982), among others. HD1375 is 57% similar to residues 1-449 from AK3_ECOLI ...
tRNA 2-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1-2cyclic phosphate and ...
Based on the results of these studies we propose that accelerated purine nucleotide synthesis and uric acid overproduction in our patient resulted from PRS superactivity due to a point mutation in PRPS1, a T-for-A substitution at nucleotide 578 in the PRPS1 coding region. This mutation has 2 distinct functional effects on the activity of the PRS1 isoform thus encoded. First, as is the case in most of the point mutations encountered to date in affected male patients (6), the mutant PRS1 in our patient appears to be more labile than its normal counterpart, resulting in reduced concentrations and activities of the mutant isoform in the patients cells and accounting for the subnormal maximum PRS activities measured in her cell extracts at maximally activating Pi concentrations. Second, the mutation in the patients PRS1 imparts altered allosteric regulatory properties on the activity of the isoform. These altered properties include increased responsiveness of enzyme activity to activation by Pi ...
TABLE-US-00003 TABLE 3 PFAM Results for Amino Acid Sequences Amino Acid SEQ ID Range NO: ORF Domain Start, Stop Family PFAM Accession No. E-value 3 877 PTS_IIA 16, 111 PTS system, Lactose/Cellobiose specific IIA PF02255 8.20E-40 subunit 5 609 PTS_EIIA_1 30, 134 phosphoenolpyruvate-dependent sugar PF00358 6.00E-55 phosphotransferase system, EIIA 1 7 1479 PRD 76, 171; PRD domain PF00874 9.90E-52 181, 282 7 1479 CAT_RBD 6, 67 CAT RNA binding domain PF03123 1.10E-16 9 1574 Glyco_hydro_1 4, 471 Glycosyl hydrolase family 1 PF00232 2.90E-133 11 1707 PTS_EIIA_1 491, 595 phosphoenolpyruvate-dependent sugar PF00358 6.10E-53 phosphotransferase system, EIIA 1 11 1707 PTS_EIIC 105, 387 Phosphotransferase system, EIIC PF02378 3.10E-33 11 1707 PTS_EIIB 7, 41 phosphotransferase system, EIIB PF00367 8.50E-19 13 725 PTS_EIIA_1 528, 632 phosphoenolpyruvate-dependent sugar PF00358 4.10E-60 phosphotransferase system, EIIA 1 13 725 PTS_EIIC 122, 419 Phosphotransferase system, EIIC PF02378 3.80E-35 13 725 PTS_EIIB 21, ...
casSAR Dugability of A2S874 | argB | Acetylglutamate kinase - Also known as ARGB_BURM9, argB. Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.
the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK2) catalyzes both the formation of fructose-2,6-bisphosphate (fructose-2,6-P2) and its degradation (26, 27, 35). Fructose-2,6-P2 is a regulator of glycolysis because it is a potent activator of phosphofructokinase-1 and inhibitor of fructose-1,6-bisphosphatase-1. Various tissue-specific isoforms of PFK2 encoded by four genes are expressed in mammals. They differ in their relative kinase and bisphosphatase activities and also in their regulatory mechanisms (36). The liver isoform is regulated by phosphorylation of a serine residue at the NH2 terminus (Ser-32) by cAMP-dependent protein kinase, which leads to an increase in the bisphosphatase-to-kinase activity ratio. This mechanism accounts for the lowering of fructose-2,6-P2 and inhibition of glycolysis caused by glucagon (26).. Recent studies by Baltrusch et al. (8) showed that PFK2 binds to glucokinase through the bisphosphatase domain. Putative roles for this ...
CsiE has two central PRD domains with histidine residues predicted to be phosphorylated by the sugar phosphotransferase system (PTS) in response to the availability of carbon source. The PRD domains of BglG phosphorylated by the sugar phosphotransferase system (PTS) in response to the availability of carbon source ...
Fructose 6 Phosphate Kinase, 0.1 mg. Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate.
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... Designation: pBSL97 TypeStrain=False Application: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance