TY - JOUR. T1 - Serine-phosphorylated STAT3 promotes tumorigenesis via modulation of RNA polymerase transcriptional activity. AU - Balic, Jesse J.. AU - Garama, Daniel J.. AU - Saad, Mohamed I.. AU - Yu, Liang. AU - West, Alison C.. AU - West, Alice J.. AU - Livis, Thaleia. AU - Bhathal, Prithi S.. AU - Gough, Daniel J.. AU - Jenkins, Brendan J.. PY - 2019/10/15. Y1 - 2019/10/15. N2 - Deregulated activation of the latent oncogenic transcription factor STAT3 in many human epithelial malignancies, including gastric cancer, has invariably been associated with its canonical tyrosine phosphorylation and enhanced transcriptional activity. By contrast, serine phosphorylation (pS) of STAT3 can augment its nuclear transcriptional activity and promote essential mitochondrial functions, yet the role of pS-STAT3 among epithelial cancers is ill-defined. Here, we reveal that genetic ablation of pS-STAT3 in the gp130F/F spontaneous gastric cancer mouse model and human gastric cancer cell line xenografts ...
In article ,31D7ED66.3535 at ens-lyon.fr,, Stephane Ory ,Stephane.Ory at ens- lyon.fr, writes: , Does anyone know where antibody against phosphoserine and , phosphothreonine are available ? , , Thanks per advance Monoclonals are available from Sigma but when we tested them, they were not useful. Phosphoserine and phosphothreonine are about 100-1000X more abundant in proteins that phosphotyrosine so you need an additional level of specificity. A number of companies are selling antibodies to proteins that only detect those proteins if a particular ser/thr/tyr residue is phosphorylated. See NEB and UBI catalogues for example. Examples of phospho-specific antibodies to the following proteins: tau (microtubule-associated protein, various ser/thr) Jun (ser 63) STAT 3 (tyr 705) SEK1 ERK1/2 (tyr 204) p38 MAPK CREB (ser 133) Jim Woodgett Ontario Cancer Institute mailto:jwoodget at oci.utoronto.ca 610 University Avenue http://kinase.oci.utoronto.ca/woodgettlab.html Toronto, Ontario M5G 2M9 phone (416) ...
Phosphoserine aminotransferase antibody for detecting human phosphoserine aminotransferase. Validated on up to 12 cell lysates for western blotting. Try a trial size today.
Recombinant human PSAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AlphaScreen SureFire cJun, p-Ser63, Assay kit enables precise investigation of signaling pathways and kinase targets in a no-wash, one well format.
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity).
AlphaScreen SureFire CHK1, pSer345 Assay Kit enables precise investigation of signaling pathways and kinase targets in a no-wash, one well format.
This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is O-phosphoserine phosphohydrolase. This enzyme participates in glycine, serine and threonine metabolism. ...
This PhosphoDetect™ Phosphoserine Detection Kit is validated for use in Immunoblotting, Immunoprecipitation, ELISA. - Find MSDS or SDS, a COA, data sheets and more information.
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
BioAssay record AID 493218 submitted by NCGC: Immunofluorescence assay for KAP-1 phosphorylation on Ser824 for Identifying a Potential Treatment of Ataxia-Telangiectasia: Hit Validation.
Goat Polyclonal Anti-Phosphoserine phosphatase Antibody. Validated: WB, PEP-ELISA. Tested Reactivity: Human, Mouse. 100% Guaranteed.
A scientific resource for the WD40 protein domain containing information on structure, function, and binding to both phospho-Ser/Thr and methylated lysine residues.
Lapin CREB1 Polyclonal anticorps pSer133 pour IHC, WB. Publier en 8 références Pubmed. Commandez anti-CREB1 anticorp. | Numéro de produit ABIN152614
Bainimarama mounted a counter-response, one that has become a permanent feature of Fijis international stance. Fiji joined the Non-Aligned Movement and extended its diplomatic reach, setting up new embassies in South Africa, Brazil, South Korea, Indonesia and the United Arab Emirates. At the UN, Fiji made the Pacific Small Islands Developing States group (eleven Pacific countries) an effective grouping, and in 2013 was chair of the G77, the UN grouping of 134 developing countries. Fiji grew notably closer to China, sending military officers to Beijing for training, accepting a significant amount of Chinese aid and welcoming investment in Fiji mining by Chinese companies. Fiji rolled out the red carpet for Xi Jinping when he visited Fiji in 2014, and Fiji soldiers participated in Chinas V-Day parade in 2015 ...
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It has been observed that coincident with or immediately following IκBα degradation, p65 is phosphorylated at multiple residues, and these phosphorylation events are necessary for proper regulation of NF-κB function (45). The phosphorylation patterns of NF-κB proteins have not been characterized in T cell anergy, and so we asked whether aberrant phosphorylation was responsible for the defects in NF-κB function in anergic cells. An early step involves phosphorylation of p65 at Ser536 by the IKK complex (32-35), and it has been suggested that phosphorylation at this residue negatively regulates the kinetics of p65 nuclear translocation (33). We found that p65 is phosphorylated at Ser536 equivalently in both naive and anergic cells, which is consistent with our finding that p65 translocates to the nucleus with normal kinetics in anergic T cells. A second posttranslational modification important for NF-κB activity is phosphorylation at Ser276. We found that, as with Ser536 phosphorylation, p65 ...
1BJO: Crystal structure of phosphoserine aminotransferase from Escherichia coli at 2.3 A resolution: comparison of the unligated enzyme and a complex with alpha-methyl-l-glutamate.
This MassBank Record with Accession UO000007 contains the MS mass spectrum of archaetidylserine with the compound class Glycerophospholipids. The mass spectrum was acquired on a FAB-B with NEGATIVE ionisation and has the SPLASH splash10-03dr-0900000000-3b77e64372833f742a03.
Gong J, Holewinski RJ, Van Eyk JE, Steinberg SF (2016) A novel phosphorylation site at Ser130 adjacent to the pseudosubstrate domain contributes to the activation of protein kinase C-δ. Biochem J 473, 311-20 ...
reacts with Akt when phosphorylated at Ser473; also reacts with Akt2 and Akt3 when phosphorylated at corresponding residues. Does not recognize Akt phosphorylated at other sites, nor does it recognize phosphorylated forms of related kinases such as PKC or p70 S6 ...
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity ...
Ubiquitin-like, containing PHD and RING finger domains 1 (uhrf1) is regulated at the transcriptional level during the cell cycle and in developing zebrafish embryos. We identify phosphorylation as a novel means of regulating UHRF1 and demonstrate that Uhrf1 phosphorylation is required for gastrulation in zebrafish. Human UHRF1 contains a conserved cyclin dependent kinase 2 (CDK2) phosphorylation site at serine 661 that is phosphorylated in vitro by CDK2 partnered with Cyclin A2 (CCNA2), but not Cyclin E. A phosphoserine-661 specific antibody recognizes UHRF1 in both in mammalian cancer cells and in non-transformed zebrafish cells, but not in zebrafish bearing a mutation in ccna2. Depleting Uhrf1 from zebrafish embryos by morpholino injection causes arrest before gastrulation and early embryonic death. This phenotype is rescued by wild-type UHRF1, but not by UHRF1 in which the phospho-acceptor site is mutated, demonstrating that UHRF1 phosphorylation is essential for embryogenesis. UHRF1 was ...
O-phospho-L-serine---tRNA ligase ( EC 6.1.1.27 , O-phosphoseryl-tRNA ligase, non-canonical O-phosphoseryl-tRNA synthetase, SepRS) is an enzyme with systematic name O-phospho-L-serine:tRNACys ligase (AMP-forming). This enzyme catalyses the following chemical reaction : In organisms like Archaeoglobus fulgidus , this enzyme ligates O-phosphoserine to tRNACys. References Fukunaga, R.; Yokoyama, S. (2007).
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
201 Page 210 202 D. 3 KID domain from CREB, phosphorylated on Ser133 Snovitra xl 60 mg in a complex with the KIX domain of CBP. AlвBadr were applied successfully for the determination of miconazole and the other anti- fungal drug in their pharmaceutical formulation. The snovitra and ventricular electrograms are on top and the s novitra markers at snovtira bottom.
C elegans SYD-2 protein: a coiled-coil phosphoserine protein; binds to the intracellular region of the LAR transmembrane PTPase; associates with focal adhesions
Maus Monoklonal ATM Antikörper pSer1981 für ICC, IF, IHC (p), IP, IHC, WB. Publiziert in 19 Pubmed Referenzen. Jetzt diesen anti-ATM Antikörper bestellen. | Produkt ABIN151772
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2) 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. For diagram, click here or here or here for mechanism click here.. Other name(s): PSAT; phosphoserine aminotransferase; 3-phosphoserine aminotransferase; hydroxypyruvic phosphate-glutamic transaminase; L-phosphoserine aminotransferase; phosphohydroxypyruvate transaminase; phosphohydroxypyruvic-glutamic transaminase; phosphoserine aminotransferase; 3-O-phospho-L-serine:2-oxoglutarate aminotransferase; SerC; PdxC; 3PHP transaminase. Systematic name: O-phospho-L-serine:2-oxoglutarate aminotransferase. Comments: A pyridoxal-phosphate protein. This enzyme catalyses the second step in the phosphorylated pathway of serine biosynthesis in Escherichia coli [2,3]. It also catalyses the third step in the biosynthesis of the coenzyme pyridoxal 5-phosphate in Escherichia coli (using Reaction 2 above) [3]. In Escherichia coli, pyridoxal 5-phosphate is synthesized de novo by a pathway that involves EC ...
The structure of the Mg(2+)-dependent enzyme human phosphoserine phosphatase (HPSP) was exploited to examine the structural and functional role of the divalent cation in the active site of phosphatases. Most interesting is the biochemical observation that a Ca(2+) ion inhibits the activity of HPSP, even in the presence of added Mg(2+). The sixfold coordinated Mg(2+) ion present in the active site of HPSP under normal physiological conditions, was replaced by a Ca(2+) ion by using a crystallization condition with high concentration of CaCl(2) (0.7 m). The resulting HPSP structure now shows a sevenfold coordinated Ca(2+) ion in the active site that might explain the inhibitory effect of Ca(2+) on the enzyme. Indeed, the Ca(2+) ion in the active site captures both side-chain oxygen atoms of the catalytic Asp20 as a ligand, while a Mg(2+) ion ligates only one oxygen atom of this Asp residue. The bidentate character of Asp20 towards Ca(2+) hampers the nucleophilic attack of one of the Asp20 side ...
The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq, Jul 2008 ...
Buy anti-WEE1 antibody, Rabbit WEE1 (Phospho-Ser642) Antibody-NP_001137448.1 (MBS001313) product datasheet at MyBioSource, Primary Antibodies. Application: ELISA (EIA), Western Blot (WB)
Generation of Phospho-Ser65 Parkin and Phospho-Thr257 PINK1 Pre-clinical Monoclonal Antibodies and Characterization of Total PINK1 Pre-clinical Monoclonal ...
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anti-GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) (pSer232), (AA 215-260) antibody (Cy5.5) ABIN801158 from antibodies-online
Regulation of type I IFN signaling through STAT2 has remained understudied chiefly because the diversity of STAT TADs and the sequence divergence of the STAT2 TAD among different species have made it challenging to predict amino acids in STAT2 that might be important for proper transcriptional activity (Park et al., 1999; Paulson et al., 1999). For example, serine phosphorylation within the P(M)SP motifs found in several STAT transactivation domains has been shown to be required for STAT-dependent gene regulation. In contrast, STAT2 lacks this conserved motif, and this opens the door to investigate the biological role of serine-phosphorylated STAT2.. Our study provides new evidence that serine phosphorylation of STAT2 plays a pivotal role in selectively regulating the biological actions of type I IFNs. We have identified a serine phosphorylation site (S734) on STAT2 that resides in the TAD, but that is away from the crucial region previously mapped for transactivation. This new site appears to ...
APP is phosphorylated at multiple sites in the 47 amino acid C-terminal cytoplasmic domain (Suzuki et al., 1994). Phosphorylation of Thr668 is particularly important, because it induces conformational changes that affect APP function and metabolism (Ando et al., 2001; Ramelot and Nicholson, 2001). To understand the mechanism of APP phosphorylation at Thr668 in neuronal cells, in this study, we investigated the role of the kinases Cdk5 and JNK and of their scaffolding and activating proteins. Using dominant-negative strategies and small molecule inhibitors, we found that JNK, not Cdk5, phosphorylates APP in differentiating neuronal cells. By preventing the interaction of JIP-1 with JNK or APP, we established that this JNK scaffolding, APP-binding protein does not participate in APP phosphorylation in neurons under normal physiological conditions. Importantly, we found that JIP-3, another JNK adaptor protein, which does not directly interact with APP, participates in the generation of a large ...
以下に掲載:Cancer Science 105 pp.1307-1312 2014. Japanese Cancer Association 共著者:Eiichi Kato, Makoto Orisaka, Tetsuji Kurokaw
G43a1 modified to contain SEP (phosphoserine), TPO (phosphothreonine), and PTR (phosphotyrosine) (all PO4^{2-} forms), and SEPH, TPOH, PTRH (PO4H^{-} forms). The charges and vdw parameters come from Hansson, Nordlund and Aqvist jmb 265 118-27 1997. They worked mostly with gromos 87 but I generally use gromos 96 so I\ve made the files in that format (there seem to be no differences in the amino acid charges between 87 and 96). Hansson et al don\t report bond angle or dihedral parameters, and I don\t know how to do QM calculations, so I\ve used the standard forms from the *bon.itp file. For the dihedrals, I\ve used the 3-fold gd_11. Because of their symmetry, the PO4^{2-} forms at least cannot have the 2-fold + 3-fold symmetry used in the gmx DNA bases. The OP-X LJ-14 parameters are the same as for OA. I\ve checked that I can build simple 1-molecule topologies and tprs for the PO4H forms, and for the PO4 forms have done 100 ps solvated MD of the isolated am acs and the am acs in proteins ...
In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.. ...
Mycobacterium avium phosphoserine phosphatase (SerB): Researchers from the Seattle Structural Genomics Center for Infectious Diseases (SSGCID)have determined the three-dimensional protein structure of Mycobacterium avium phosphoserine phosphatase (SerB). The pathogen M. avium is a hardy, aerobic, Gram-positive bacterium that causes three different illnesses in humans - glandular fever in children, pneumonia in patients prone to lung disease, and generalized infection in AIDS patients. Phosphoserine phosphatate plays a vital role in amino acid metabolism in all organisms. SerB catalyzes the reaction of 3-phosphoserine to L-serine, the final step in the biosynthesis of serine in both animals and bacteria. An unexpected feature of the SerB crystal structure was the observation of two new not observed in any other SerB crystal structures. This dissimilarity to homologous SerB enzymes necessitated the use of de novo phasing. We have adopted the use of iodide ion soaks and single wavelength anomalous
A large body of work has implicated the activity of various kinases and phosphatases in the regulation of synaptic transmission by controlling the phosphorylation state of several synaptic proteins (for review, see Turner et al., 1999). To further understand the physiological significance of these modifications and to gain insights into their role in modulating synaptic strength and plasticity, we have generated a set of antibodies that specifically recognize synaptic proteins only in their phosphorylated form. In this study we report the biochemical characterization of the modulation of phosphorylation of rabphilin, a synaptic protein implicated in exocytosis, using phosphospecific antibodies directed against the two major phosphorylation sites of rabphilin, serine-234 and serine-274.. Our results show that the phosphorylation of rabphilin at serine-234 is greatly stimulated (about sevenfold over basal) by activation of PKA and high K+-induced membrane depolarization, a condition that mimics ...
In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.. ...
Rabbit polyclonal Phosphoserine phosphatase antibody validated for WB and tested in Human. Immunogen corresponding to synthetic peptide
Phosphoprotein phosphatases, which hydrolyze the phosphoester bonds of phosphoserines, phosphothreonines or phosphotyrosines, play an essential role in signal transduction and actively contribute to the regulation of protein phosphorylation. On the basis of their substrate specificity they are usually divided into phosphoserine and phosphothreonine phosphatases on the one hand, and phosphotyrosine and dual-specificity phosphatases, on the other hand. This division corresponds also to different families of enzymes with different catalytic mechanisms. Genes coding for phosphoserine/threonine phosphatases are less numerous in vertebrate genomes than those for serine /threonine kinases, and the complexity of phosphatases function arises in part from the interactions of catalytic subunits with other proteins.. Prior to the knowledge of their sequence, phosphoserine/threonine phosphatases were classified on the basis of their substrate preference and inhibitor sensitivity. Type 1 protein phosphatases ...
Simon Penson with swanson at nature.berkeley.edu (Sarah Swanson) on Mon, 23 Jan 1995 09:01:23 wrote , Im looking at the possibility of using antibodies to phosphorylated amino , acids as an alternative to radiolabelling in studies of protein , phosphorylation. Has anyone got any tips/comments about this, especially , regarding the specificity of the antibodies. , , Thanks, , , Simon Penson , Plant Biology Dept, , UC Berkeley. , Simon: Antiphosphotyrosine is widely and successfully used, especially for immunoprecipitation and Western Blotting. My student Bob Wheelock has tested a number of different commercially-available antibodies and found that monoclonal 4G10 from Upstate Biotechnology was the most efficient at immunoprecipitating and blotting phosphoproteins from mouse and human cell lines. Proteins can be eluted from precipitates easily and gently with phenyl phosphate. WRT specificity, we do occasionally find a band or 2 on gels that antiPT binds to even in the presence of phenyl ...
TY - JOUR. T1 - Pro-tumorigenic phosphorylation of p120 catenin in renal and breast cancer. AU - Kourtidis, Antonis. AU - Yanagisawa, Masahiro. AU - Huveldt, Deborah. AU - Copland, John A.. AU - Anastasiadis, Panos Z.. PY - 2015/6/11. Y1 - 2015/6/11. N2 - Altered protein expression and phosphorylation are common events during malignant transformation. These perturbations have been widely explored in the context of E-cadherin cell-cell adhesion complexes, which are central in the maintenance of the normal epithelial phenotype. A major component of these complexes is p120 catenin (p120), which binds and stabilizes E-cadherin to promote its adhesive and tumor suppressing function. However, p120 is also an essential mediator of pro-tumorigenic signals driven by oncogenes, such as Src, and can be phosphorylated at multiple sites. Although alterations in p120 expression have been extensively studied by immunohistochemistry (IHC) in the context of tumor progression, little is known about the status and ...
Concepts regarding the controls and consequences of PKD1-Ser738/Ser742 (activation loop) phosphorylation are based largely on early studies that used an anti-PKD1-Ser(P)738/Ser(P)742 PSSA (from Cell Signaling Technology, Danvers, MA) and showed that PMA increases PKD1 activation loop phosphorylation in many cell types via a mechanism that requires nPKC isoform activity (PKCδ, PKCε, PKCη, and/or PKCθ). In vitro kinase assays showing direct phosphorylation of the PKD1 activation loop by certain nPKC isoforms also have been published (Brändlin et al., 2002). However, there is recent evidence that the Cell Signaling Technology anti-PKD1-Ser(P)738/Ser(P)742 PSSA primarily recognizes PKD1 phosphorylation at Ser738 and that PKD1 phosphorylation at Ser742 can be tracked with a different PSSA (commercially available from Abcam Inc., Cambridge, MA). Experiments that use a combined approach with these two PSSAs expose differences in the controls and consequences of PKD1 phosphorylation at Ser738 and ...
The PRC leaderships decision to devote not inconsiderable resources to legal development seems premised on the notion that such development is correlated to overall economic development. To wit, law, legal institutions and legal personnel of the type described in part one are seen as having the potential to foster economic growth and, in particular, foreign involvement in the economy and are to be encouraged for that reason, among others. This is, of course, hardly a new thought. Weber compensated for many a sociological error (as, for example, in his accounts of Chinese life) by explaining how legal rationality spurred the development of capitalist societies by facilitating the predictability he indicates business so prizes (Rheinstein, 1954:29-30). North earned the Nobel Prize by pushing this line of thought further in demonstrating how relative clarity in and security of property rights, spawned in important part through formal legal institutions, had been a prime factor in the rise of major ...