The phosphorylated pathway has been proposed as the major source of serine in Neurospora Crassa (Sojka & Garner, 1967). Chuck (1980) demonstrated that a serine deficient mutant of Neurospora crassa, ser (JBM5), which is isogenic with its progenitor prototrophic strain, shows a marked decrease in its phosphoserine phosphatase specific activity when compared to the activity of its prototrophic strain. This lowered activity in the enzyme catalyzing the terminal step of the phosphorylated pathway of serine biosynthesis in a serine requiring mutant which has a single gene difference compared to its prototrophic strain supported the phosphorylated pathway as the major source of serine in Neurospora crassa. The present study assayed the phosphoserine phosphatase activities of two allelic serine requiring mutants of Neurospora crassa, ser-3 and ser (JBM5). In all assays, both mutants had significantly lower phosphoserine phosphatase specific activities than did their respective prototrophic strains. ...

TY - JOUR. T1 - Microenvironment regulates the expression of MIR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia. AU - Carabia, Júlia. AU - Carpio, Cecilia. AU - Abrisqueta, Pau. AU - Jiménez, Isabel. AU - Purroy, Noelia. AU - Calpe, Eva. AU - Palacio, Carles. AU - Bosch, Francesc. AU - Crespo, Marta. PY - 2017/12/1. Y1 - 2017/12/1. N2 - © 2017 The Author(s). Chronic lymphocytic leukemia (CLL) cells are highly dependent on microenvironment, being the BCR pathway one key player in this crosstalk. Among proteins participating, ZAP-70 enhances response to microenvironmental stimuli. MicroRNA-21 (miR-21) is overexpressed in diverse neoplasias including CLL, where it has been associated to refractoriness to fludarabine and to shorter time to progression and survival. To further elucidate the role of ZAP-70 in the biology of CLL, we studied its involvement in miR-21 regulation. MiR-21 expression was higher in CLL cells with high ZAP-70. Ectopic ...

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Antibodies for proteins involved in inositol phosphate phosphatase activity pathways, according to their Panther/Gene Ontology Classification

Fingerprint Dive into the research topics of Dimerization of inositol monophosphatase Mycobacterium tuberculosis SuhB is not constitutive, but induced by binding of the activator Mg,sup,2+,/sup,. Together they form a unique fingerprint. ...

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is O-phosphoserine phosphohydrolase. This enzyme participates in glycine, serine and threonine metabolism. ...

Inpp4a - Inpp4a (Myc-DDK-tagged) - Mouse inositol polyphosphate-4-phosphatase, type I (Inpp4a), transcript variant 1 available for purchase from OriGene - Your Gene Company.

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To determine if the SHIP phosphatase activity is sufficient to alter neutrophil motility, we ectopically expressed a membrane-bound form of the human SHIP1 phosphatase domain (aa364-826) in zebrafish neutrophils (Freeburn et al., 2002). Transient expression was achieved using the lyz promoter driving both the constitutively active SHIP1 phosphatase and EGFP expression with the viral 2A peptide system which allows multiple protein products to be expressed from a single transgene (Provost et al., 2007). Transient expression of this construct in Tg(mpx:mCherry) embryos allowed for mosaic expression of the phosphatase domain labeled with EGFP and mCherry to be compared to control neutrophils that expressed mCherry alone. Live imaging of neutrophil random motility in the head of 3 dpf embryos showed that ectopic expression of the SHIP1 phosphatase domain impaired neutrophil random motility as compared to control neutrophils (Fig. 4D-E; supplementary material Movie 7). By contrast, ectopic expression ...

The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq, Jul 2008 ...

Metastasis is the major cause of breast cancer mortality. Phosphoinositide 3-kinase (PI3K) generated PtdIns(3,4,5)P3 activates AKT, which promotes breast cancer cell proliferation and regulates migration. To date, none of the inositol polyphosphate 5-phosphatases that inhibit PI3K/AKT signaling have been reported as tumor suppressors in breast cancer. Here, we show depletion of the inositol polyphosphate 5-phosphatase PIPP (INPP5J) increases breast cancer cell transformation, but reduces cell migration and invasion. Pipp ablation accelerates oncogene-driven breast cancer tumor growth in vivo, but paradoxically reduces metastasis by regulating AKT1-dependent tumor cell migration. PIPP mRNA expression is reduced in human ER-negative breast cancers associated with reduced long-term outcome. Collectively, our findings identify PIPP as a suppressor of oncogenic PI3K/AKT signaling in breast cancer.. » Online Version. ...

The small GTPase Rac1 is thought to play an important role in cell migration and invasion. We have previously identified synaptojanin 2, a phosphoinositide phosphatase, as an effector of Rac1. Here, we show that small interfering RNA-mediated depletion of either Rac1 or synaptojanin 2 inhibits invasion of SNB19 and U87MG glioblastoma cells through Matrigel and rat brain slices. Depletion of Rac1 or synaptojanin 2 also inhibits migration of SNB19 and U87MG cells on glioma-derived extracellular matrix. In addition, we found that depletion of Rac1 or synaptojanin 2 inhibits the formation of lamellipodia and invadopodia, specialized membrane structures that are thought to be involved in extracellular matrix degradation. These results suggest that synaptojanin 2 contributes to the role of Rac1 in cell invasion and migration by regulating the formation of invadopodia and lamellipodia. This study also identifies synaptojanin 2 as a novel potential target for therapeutic intervention in malignant ...

A new study shows that 43 percent of pre-menopausal women carry mutations in the PTEN tumor suppressor gene that may predispose them to uterine cancer. The mutations occur in seemingly normal endometrial tissue, and researchers suggest that along with other genetic markers the PTEN gene may be useful for determining a womans risk of developing the disease.. In more than 50 percent of uterine cancers the PTEN tumor suppressor gene is inactive due to mutations within the gene or because the gene has been completely deleted. Researchers led by George Mutter, of Brigham and Womens Hospital in Boston, Massachusetts, found that because the lining of the uterus is not completely shed during menses, endometrial cells carrying PTEN mutations are able to persist in the uterus. The endometrial tissue is made up of rapidly dividing cells, which facilitates the proliferation of cells carrying genetic mutations. When using conventional bulk tissue-handling methods, these abnormal cells are easily ...

Pseudomonas aeruginosa arylsulfatase (PAS) is a bacterial sulfatase capable ofhydrolyzing a range of sulfate esters. Recently, it has been demonstrated to also show very high proficiency for phosphate ester hydrolysis. Such proficient catalytic promiscuity is significant, as promiscuity has been suggested to play an important role in enzyme evolution. Additionally, a comparative study of the hydrolyses of the p-nitrophenyl phosphate and sulfate monoesters in aqueous solution has demonstrated that despite superficial similarities, the two reactions proceed through markedly different transition states with very different solvation effects, indicating that the requirements for the efficient catalysis of the two reactions by an enzyme will also be very different (and yet they are both catalyzed by thesame active site). This work explores the promiscuous phosphomonoesterase activity ofPAS. Specifically, we have investigated the identity of the most likely base for the initial activation of the ...

14-3-3 proteins belong to a family of conserved molecules, which play a regulatory role and participate in signal transduction and checkpoint control pathways. 14-3-3 proteins bind phosphoserine-phosphorylated ligands, such as the Raf-1 kinase and Bad, through recognition of the phosphorylated consensus motif, RSXpSXP (where pS is phosphoserine). Recently, a phosphorylation-independent interaction has been reported to occur between 14-3-3 and a small number of proteins, for example the 43 kDa inositol polyphosphate 5-phosphatase, glycoprotein Ib, p75NTR-associated cell-death executor (NADE) and the bacterial ADP-ribosyltransferase toxin exoenzyme S (ExoS). It has been suggested that specific residues of 14-3-3 proteins are required for activation of the bacterial toxin ExoS. An unphosphorylated peptide derived from a phage display library, known as the R18 peptide, and a synthetic peptide derived from ExoS inhibit the interaction between ExoS and 14-3-3. In this report we identify the amino acid ...

HER2/neu overexpression due to gene amplification is an important factor in breast cancer, modifying the sensitivity to anti-HER2 monoclonal antibody therapy. The clinical significance of HER2 expression in non small cell lung carcinoma (NSCLC) is currently under evaluation. The tumor suppressor gene PTEN negatively regulates the HER2/PI3K/Akt signalling pathway. The purpose of this study was to evaluate the role of simultaneous alteration in HER2 and PTEN protein expression in relation to biological behaviour of NSCLCs.. MATERIALS AND METHOD:. Protein expression was determined by immunohistochemistry in 82 NSCLC cases along with CISH for HER2 gene analysis and detection of chromosome 17 aneuploidy. Patients were followed-up for a period of 34 to 41 months after surgery.. RESULTS:. HER2 overexpression (2+/3+ score) was detected in 23 (27.9%) patients while loss of PTEN expression was observed in 32 (39.3%) cases, low expression in 39 (47.6%) and overexpression in 11(13.1%). Simultaneous HER2 ...

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Crystal structure of the cytoplasmic phosphatase and tensin homolog (PTEN)-like region of Ciona intestinalis voltage-sensing phosphatase provides insight into substrate specificity and redox regulation of the phosphoinositide phosphatase ...

TY - JOUR. T1 - Substrate recognition by the human MTH1 protein.. AU - Kamiya, Hiroyuki. AU - Dugué, Laurence. AU - Yakushiji, Hiroyuki. AU - Pochet, Sylvie. AU - Nakabeppu, Yusaku. AU - Harashima, Hideyoshi. N1 - Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine. PY - 2002. Y1 - 2002. N2 - A nucleotide pool sanitizing enzyme, the human MTH1 protein, hydrolyzes 2-hydroxy-dATP, 8-hydroxy-dATP, and 8-hydroxyd-GTP. To examine the substrate recognition mechanism of the MTH1 protein, ten nucleotide analogs (8-bromo-dATP, 8-bromod-GTP, deoxyisoinosine triphosphate, 8-hydroxy-dITP, 2-aminopurine-deoxyriboside triphosphate, 2-amino-dATP, deoxyxanthosine triphosphate, deoxyoxanosine triphosphate, dITP, and dUTP) were incubated with the protein. Of these, the former five nucleotides were hydrolyzed with various efficiencies. This results suggests the importance of the anti/syn-conformation and the functional groups on the 2 and 6-positions of the ...

TY - JOUR. T1 - Metabolic switching of PI3K-dependent lipid signals. AU - Downes, Pete. AU - Leslie, N. R.. AU - Batty, I. H.. AU - van der Kaay, J.. PY - 2007. Y1 - 2007. N2 - The lipid phosphatase, PTEN (phosphatase and tensin homologue deleted on chromosome 10), is the product of a major tumour suppressor gene that antagonizes PI3K (phosphoinositide 3-kinase) signalling by dephosphorylating the 3-position of the inositol ring of PtdIns(3,4,5)P(3). PtdIns(3,4,5)P(3) is also metabolized by removal of the 5-phosphate catalysed by a distinct family of enzymes exemplified by SHIP1 [SH2 (Src homology 2)-containing inositol phosphatase 1] and SHIP2. Mouse knockout studies, however, suggest that PTEN and SHIP2 have profoundly different biological functions. One important reason for this is likely to be that SHIP2 exists in a relatively inactive state until cells are exposed to growth factors or other stimuli. Hence, regulation of SHIP2 is geared towards stimulus dependent antagonism of PI3K ...

ID INM1_YEAST Reviewed; 295 AA. AC P38710; D3DKZ4; DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1995, sequence version 1. DT 22-NOV-2017, entry version 142. DE RecName: Full=Inositol monophosphatase 1; DE Short=IMP 1; DE Short=IMPase 1; DE EC=3.1.3.25; DE AltName: Full=Inositol-1(or 4)-monophosphatase 1; GN Name=INM1; Synonyms=IMP1; OrderedLocusNames=YHR046C; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=8091229; DOI=10.1126/science.8091229; RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., RA Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., RA Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., RA Latreille P., Louis E.J., Macri C., Mardis E., Menezes S., ...

DNA bases can be modified by endogenous agents (e.g. oxidized by products of respiration and photosynthesis or methylated by gene silencing processes) as well as by environmental agents (e.g. oxidized by UV light). In the process of removing modified bases, a 3-phosphate group is sometimes left in the resulting gap, and has to be removed since it blocks the incorporation of a new nucleotide by DNA polymerase. The aim of this thesis was the characterization of AtZDP, a plant enzyme with a DNA 3-phosphatase activity.. By homologous modeling, the existence of four domains was predicted in AtZDP, three independent zinc-finger and one DNA 3-phosphatase domains. AtZDP was found to be localized in the nucleus by bimolecular fluorescence complementation. Western blotting analysis showed that the enzyme was ubiquitously expressed in plant tissues.. AtZDP was found in a 600,000 molecular-weight protein complex by gel chromatography and glycerol gradient sedimentation centrifugation. The fractions ...

Inp53 is a calcineurin substrate. (A) Domain structure of yeast synaptojanins and creation of Inp53ARAQAA allele. IP5P, inositol-polyphosphate 5-phosphatase dom

FUNCTION: The protein encoded by this gene is a lipid phosphate phosphohydrolase. It is an integral membrane protein that catalyzes the conversion of phosphatidic acid to diacylglycerol and inorganic phosphate. The transcript is expressed at high levels in lung, liver, and kidney and at low levels in brain and heart. Null mutant mice are viable and fertile and display no overt phenotypic defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014 ...

The PI3K signaling pathway regulates cell growth and movement and is heavily mutated in cancer. Class I PI3Ks synthesize the lipid messenger PI(3,4,5)P3. PI(3,4,5)P3 can be dephosphorylated by 3- or 5-phosphatases, the latter producing PI(3,4)P2. The PTEN tumor suppressor is thought to function primarily as a PI(3,4,5)P3 3-phosphatase, limiting activation of this pathway. Here we show that PTEN also functions as a PI(3,4)P2 3-phosphatase, both in vitro and in vivo. PTEN is a major PI(3,4)P2 phosphatase in Mcf10a cytosol, and loss of PTEN and INPP4B, a known PI(3,4)P2 4-phosphatase, leads to synergistic accumulation of PI(3,4)P2, which correlated with increased invadopodia in epidermal growth factor (EGF)-stimulated cells ...

The PI3K signaling pathway regulates cell growth and movement and is heavily mutated in cancer. Class I PI3Ks synthesize the lipid messenger PI(3,4,5)P3. PI(3,4,5)P3 can be dephosphorylated by 3- or 5-phosphatases, the latter producing PI(3,4)P2. The PTEN tumor suppressor is thought to function primarily as a PI(3,4,5)P3 3-phosphatase, limiting activation of this pathway. Here we show that PTEN also functions as a PI(3,4)P2 3-phosphatase, both in vitro and in vivo. PTEN is a major PI(3,4)P2 phosphatase in Mcf10a cytosol, and loss of PTEN and INPP4B, a known PI(3,4)P2 4-phosphatase, leads to synergistic accumulation of PI(3,4)P2, which correlated with increased invadopodia in epidermal growth factor (EGF)-stimulated cells ...

Catalyzes the dephosphorylation of an unusually broad range of substrate including deoxyribo- and ribonucleoside tri-, di-, and monophosphates, as well as polyphosphate and glucose-1-P (Glu1P).

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

Aliases : GRMZM2G369130. Description : 28.1 DNA.synthesis/chromatin structure Encodes an inositol polyphosphate 5Ã--phosphatase (5PTase). Mediating phosphoinositide signaling. Involved in establishment of foliar vein patterns. CVP2 like 1 (CVL1). ...

Complete information for SYNJ2BP gene (Protein Coding), Synaptojanin 2 Binding Protein, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

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Recent experiments show that PTEN may well dephosphorylate the 3 phosphate associated with PI(3, 4, 5)P3in vitro. We therefore examined when PTEN mutations could explain the increased amounts of 3 phospholipids and this increased PKB/Akt activity with GM cell lines. … Continue reading →. ...

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References for Abcams Recombinant Human IMPA2 protein (ab104020). Please let us know if you have used this product in your publication

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Oculocerebrorenal syndrome (also called Lowe syndrome) is a rare X-linked recessive disorder characterized by congenital cataracts, hypotonia, intellectual disability, proximal tubular acidosis, aminoaciduria, and low-molecular-weight proteinuria. Lowe syndrome can be considered a cause of Fanconi syndrome (bicarbonaturia, renal tubular acidosis, potassium loss, and sodium loss). Because oculocerebrorenal syndrome is an X-linked recessive condition, the disease develops mostly in men with very rare occurrences in women, while women are carriers of the disease; it has an estimated prevalence of 1 in 500,000 people. Boys with Lowe syndrome are born with cataracts in both eyes, glaucoma is present in about half of the individuals with Lowe syndrome, though usually not at birth. While not present at birth, many affected boys develop kidney problems at about one year of age. Renal pathology is characterized by an abnormal loss of certain substances into the urine, including bicarbonate, sodium, ...

Oculocerebrorenal syndrome of Lowe (OCRL) is a rare X-linked disorder characterized by congenital cataracts, mental retardation, and renal tubular dysfunction. Patients with known or suspected OCRL were enrolled under previous protocol 96-HG-0008 that expired in 1998 and was not renewed. We are continuing studies of DNA and cell samples obtained and archived under our previous protocol to identify mutations in the OCRL1 gene responsible for Lowe syndrome and related disorders in affected males and attempt to correlate these mutations to particular biochemical or cellular phenotypes (enzyme activity, protein stability, cellular localization and trafficking). Information about genotypes will not be communicated back to the patients, their parents (if patient is a minor) or their physicians as part of this study.. We are also continuing our investigations of heterogeneity in OCRL by studying collected cell cultures from our collaborator Dr. Steven Scheinman at Suny New York Medical University, ...

Oculocerebrorenal syndrome of Lowe (OCRL) is an X-linked recessive disorder. This study investigated the history of a Chinese family with OCRL and used direct DNA sequencing to screen all exons of OCRL gene for mutations. A missense mutation (1736 A→G) in exon 15 was revealed, which .... ...

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The study aim was to investigate the impacts of the expressions of tumor suppressor gene phosphatase and tensin homolog deleted on chromosome ten (PTE..

TY - JOUR. T1 - A motor driving PTEN. AU - Zhou, Jing. AU - Parada, Luis F.. N1 - Copyright: Copyright 2009 Elsevier B.V., All rights reserved.. PY - 2009. Y1 - 2009. N2 - To fulfil its lipid phosphatase function, PTEN must be in close proximity to the plasma membrane where its substrates reside. PTEN translocation to the plasma membrane is an active process that is mediated by the myosin-based transport machinery. MyosinV controls PTEN membrane association and thus, PTEN-mediated cell growth in neurons.. AB - To fulfil its lipid phosphatase function, PTEN must be in close proximity to the plasma membrane where its substrates reside. PTEN translocation to the plasma membrane is an active process that is mediated by the myosin-based transport machinery. MyosinV controls PTEN membrane association and thus, PTEN-mediated cell growth in neurons.. UR - http://www.scopus.com/inward/record.url?scp=70349636049&partnerID=8YFLogxK. UR - ...

SH2-containing inositol phosphatase, SH protein-tyrosine phosphatase, SH domains, SH, Online Electronic Medical Dictionary Terminology, Articles, Glossary

Somatic ribosomal protein mutations have recently been described in cancer, yet their impact on cellular transcription and translation remains poorly understood. Here, we integrate mRNA sequencing, ribosome footprinting, polysomal RNA sequencing and mass spectrometry datasets from a mouse lymphoid cell model to characterize the T-cell acute lymphoblastic leukemia (T-ALL) associated ribosomal RPL10 R98S mutation. Surprisingly, RPL10 R98S induces changes in protein levels primarily through transcriptional rather than translation efficiency changes. Phosphoserine phosphatase (PSPH), encoding a key serine biosynthesis enzyme, was the only gene with elevated transcription and translation leading to protein overexpression. PSPH upregulation is a general phenomenon in T-ALL patient samples, associated with elevated serine and glycine levels in xenograft mice. Reduction of PSPH expression suppresses proliferation of T-ALL cell lines and their capacity to expand in mice. We identify ribosomal mutation ...

Phosphoinositides have traditionally been known to be important in the generation of the second messengers inositol 1,4,5,-triphosphate and diacylglycerol. Recently, it was demonstrated that in yeast and animals, phosphoinositides themselves are regulators of a wide variety of cellular processes, such as signal transduction, actin cytoskeleton organization, vesicle trafficking, and activation of proteins such as phosphoinositide-dependent kinase 1 and phospholipase D (Martin, 1998; Takenawa and Itoh, 2001). In plant cells, all phosphoinositide forms except phosphatidylinositol 3,4,5-triphosphate [PtdIns(3,4,5)P3] have been identified, and they have been suggested to play important roles in vesicle trafficking (Matsuoka et al., 1995; Kim et al., 2001), pollen tube growth (Kost et al., 1999), and stress and hormone responses (Mikami et al., 1998; Meijer et al., 1999, 2001; Pical et al., 1999; DeWald et al., 2001). PtdIns(4,5)P2 has been shown to bind profilin (Kovar et al., 2001) and to regulate ...

Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base.: Escherichia coli MutT hydrolyzes 8-

Myosin 1E is one of two long-tailed human Class I myosins that contain an SH3 domain within the tail region. SH3 domains of yeast and amoeboid myosins I interact with activators of the Arp2/3 complex, an important regulator of actin polymerization. No binding partners for the SH3 domains of myosin …

Les kinases et les phosphatases (KP) représentent la plus grande famille des enzymes dans la cellule. Elles régulent les unes les autres ainsi que 60 % du protéome, formant des réseaux complexes kinase-phosphatase (KP-Net) jouant un rôle essentiel dans ...

PTEN expression induced cell cycle arrest in endometrial carcinoma cells lacking endogenous PTEN, whereas the growth of a cell line containing wild-type PTEN was unaffected. In AN3CA and RL-95 cells, inhibition of growth paralleled decreases in the levels of phospho-AKT. In contrast, growth of Hec1A cells was unaffected by PTEN expression despite decreased levels of phospho-AKT. However, the levels of phospho-AKT were already very low in these cells. It is likely that Hec1A cells have sustained an alternative mutation(s) downstream of AKT that contributed to tumorigenesis in the presence of persistent wild-type PTEN. In agreement with this possibility, the downstream effects of PTEN expression, decreased cyclin D3 levels and a concomitant increase in association of p27KIP1 with CDK2, were not observed in Hec1A cells. Because all tumors and tumor cell lines contain multiple mutations, it is possible that not all endometrial tumors that contain wild-type PTEN would have the same resistance to PTEN ...

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LPS), have been shown to block neutrophilspontaneous death (4-7, 26, 27). We have confirmed theantineutrophil death effect of these reagents in our experimentalsystem (Fig. 4A). In addition, we showed that these factorsalso can prevent deactivation of Akt during neutrophil deathwith no alterations in total Akt levels (Fig. 4 B and C). Theseresults are consistent with our hypothesis that Akt deactivationis an essential causal mediator in neutrophil spontaneous death.. Augmentation of PtdIns(3,4,5)P3/Akt Signal by PTEN Depletion Prevents Neutrophil Spontaneous Death. If Akt deactivation mediates neutrophil spontaneous death, thenelevating this pathway should diminish neutrophil death. Totest this idea, we tried to augment PtdIns(3,4,5)P3/Akt signalingby disrupting PTEN gene. The tumor suppressor PTEN is a phosphatidylinositol3-phosphatase that converts PtdIns(3,4,5)P3 to PtdIns(4,5)P2.Depletion of this lipid phosphatase leads to accumulation ofPtdIns(3,4,5)P3 on the plasma membrane and, thus, ...

Clone REA580 recognizes the human and mouse SHIP-1 antigen, which is a 145 kDa protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. SHIP-1, also known as enzyme phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1, is expressed on hematopoietic cells, including immature CD34+ cells, the majority of T cells, and a minority of B cells. It has a negative effect on cell growth and therefore loss or modification may have profound effects on hematopoietic cell development. Thus SHIP-1 functions as a negative regulator of myeloid cell proliferation and survival.Additional information: Clone REA580 displays negligible binding to Fc receptors. | USA

Clone REA270 recognizes the phosphatase and tensin homolog deleted on chromosome ten (PTEN) antigen, regardless of phosphorylation status. PTEN is a tumor suppressor gene which is also known as mutated in multiple advanced cancers 1 (MMAC1) or TGF-β-regulated and epithelial cell-enriched phosphatase (TEP-1). It has been found to be defective in a large number of human cancers like glioblastoma, breast, and prostate. In addition, germ-line mutations in PTEN result in the dominantly inherited disease Cowden syndrome. PTEN functions as a negative regulator of the phosphoinositide 3-kinase signaling pathway which has crucial roles in cell proliferation, survival, differentiation, and migration. The protein encoded by PTEN is a phosphatase that preferentially dephosphorylates phosphoinositide substrates.Additional information: Clone REA270 displays negligible binding to Fc receptors. | USA

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Description: SH2-Domain containing inositol 5-phosphatases (SHIP1 & SHIP2) dephosphorylate the 5-position of PI(3,4,5)P3 generating PI(3,4)P2. PI(3,4)P2 activates Protein Kinase B (PKB/Akt) and promotes cell survival. SHIP2 expression and activity is increased in colorectal cancer suggesting an oncogenic role. Treatment of colorectal cancer cells with K149 (2uM) decreases PKB/Akt signaling and sensitizes the cells to 5-fluorouracil regardless of whether the cell lines have a PI3KCA mutation ...

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MEDIZINISCHE MOLEKULARGENETIK; AMYOTROPHISCHE LATERALSKLEROSE (NEUROPATHOLOGIE); PHOSPHOMONOESTER-HYDROLASEN (ENZYME); MASSENSPEKTROSKOPIE + MASSENSPEKTROMETRIE (BIOLOGISCHE TECHNIKEN); TIERISCHE MODELLE IN DER MEDIZIN; MOLECULAR MEDICAL GENETICS; AMYOTROPHIC LATERAL SCLEROSIS (NEUROPATHOLOGY); PHOSPHORIC MONOESTER HYDROLASES (ENZYMES); MASS SPECTROSCOPY + MASS SPECTROMETRY (BIOLOGICAL TECHNIQUES); ANIMAL MODELS IN ...

The bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) was recently identified as a new intracellular binding partner for glucokinase (GK). Therefore, we studied the importance of this interaction for the activity status of GK and glucose metabolism in insulin-producing cells by overexpression of the rat liver and pancreatic islet isoforms of PFK-2/FBPase-2. PFK-2/FBPase-2 ...

Principal Investigator：EGUCHI SATOSHI, Project Period (FY)：2014-04-01 - 2017-03-31, Research Category：Grant-in-Aid for Scientific Research (C), Section：一般, Research Field：Structural biochemistry

MarineTraffic Live Ships Map. Discover information and vessel positions for vessels around the world. Search the MarineTraffic ships database of more than 550000 active and decommissioned vessels. Search for popular ships globally. Find locations of ports and ships using the near Real Time ships map. View vessel details and ship photos.

MarineTraffic Live Ships Map. Discover information and vessel positions for vessels around the world. Search the MarineTraffic ships database of more than 550000 active and decommissioned vessels. Search for popular ships globally. Find locations of ports and ships using the near Real Time ships map. View vessel details and ship photos.

MarineTraffic Live Ships Map. Discover information and vessel positions for vessels around the world. Search the MarineTraffic ships database of more than 550000 active and decommissioned vessels. Search for popular ships globally. Find locations of ports and ships using the near Real Time ships map. View vessel details and ship photos.

α,α-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15); Glucosylglycerol-phosphate synthase (EC 2.4.1.213); trehalose-6-P phosphatase (EC 3.1.3.12); [retaining] GDP-valeniol: validamine 7-phosphate valeniolyltransferase (EC 2.-.-.- ...

α,α-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15); Glucosylglycerol-phosphate synthase (EC 2.4.1.213); trehalose-6-P phosphatase (EC 3.1.3.12); [retaining] GDP-valeniol: validamine 7-phosphate valeniolyltransferase (EC 2.-.-.- ...

The 61,321gt ship is one of, if not the largest car carrier to call in South Africa so far, with a loading capacity of 6,800 motor vehicles. She is one of three ships in the class and was built in Japan. Tombarra is also one of 42 new builds under construction for the Swedish-Norwegian partnership that includes Eukor and American Ro-Ro carriers (ARC). Only two ships have so far been delivered the 42 will ultimately be divided among the three associated companies ...

Free Essays from Bartleby | SHIPS Since the end of the age of sail a ship has been any large buoyant watercraft. Ships are generally distinguished from boats...

Something about a ship in a bottle doesnt seem to fit. Oh, thats it - the ship! Learn the secret to create your own ship in a bottle.

Something about a ship in a bottle doesnt seem to fit. Oh, thats it - the ship! Learn the secret to create your own ship in a bottle.

Intercad cooperates with Shipyards, Ship owners and Ship Design companies around the world offering quality shipdesign services according to the latest innovations and regulations. ...

Fast-paced block stacking VR fun in a playful factory setting. Pack a box with colorful shapes, then ship it to earn your wage. Filling VR boxes is physically satisfying as you grab blocks, rotate them with you hands, and smash them home.

At Umeå Marine Sciences Centre (UMF), ships and boats are available for research, education and environmental monitoring. Universities, colleges and authorities are able to use them at subsidised prices. UMF conducts regular sampling activities all year long, and there is often the possibility of coordinating sampling. ...

I know, I know, by this point you want Just Ship It to be an actual person so you could punch it in the face. As an Indie Maker, that sentence can be...

I know, I know, by this point you want Just Ship It to be an actual person so you could punch it in the face. As an Indie Maker, that sentence can be...

Nearby ships reported receiving a horrifying distress call. All officers including captain dead, lying in chartroom and on bridge