Tay, B S.; Lilley, R M.; Murray, A W.; and Atkinson, M R., "Inhibition of phosphoribosyl pyrophosphate amidotransferase from ehrlich ascites-tumour cells by thiopurine nucleotides." (1969). Subject Strain Bibliography 1969. 1170 ...
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The pentose phosphate pathway is a process of glucose turnover that produces NADPH as reducing equivalents and pentoses as essential parts of nucleotides. There are two different phases in the pathway. One is irreversible oxidative phase in which glucose-6P is converted to ribulose-5P by oxidative decarboxylation, and NADPH is generated [MD:M00006]. The other is reversible non-oxidative phase in which phosphorylated sugars are interconverted to generate xylulose-5P, ribulose-5P, and ribose-5P [MD:M00007]. Phosphoribosyl pyrophosphate (PRPP) formed from ribose-5P [MD:M00005] is an activated compound used in the biosynthesis of histidine and purine/pyrimidine nucleotides. This pathway map also shows the Entner-Doudoroff pathway where 6-P-gluconate is dehydrated and then cleaved into pyruvate and glyceraldehyde-3P [MD:M00008 ...
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Buy our Recombinant Human Phosphoribosyl pyrophosphate amidotransferase protein. Ab159164 is a full length protein produced in Wheat germ and has been…
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Organellar and Cytosolic Localization of Four Phosphoribosyl Diphosphate Synthase Isozymes in Spinach: Four cDNAs encoding phosphoribosyl diphosphate (PRPP) syn
PRPSAP2 antibody [1E3] (phosphoribosyl pyrophosphate synthetase-associated protein 2) for FACS, ICC/IF, IHC-P, WB. Anti-PRPSAP2 mAb (GTX83790) is tested in Human samples. 100% Ab-Assurance.
PRPSAP2 antibody [N2C3] (phosphoribosyl pyrophosphate synthetase-associated protein 2) for IHC-P, WB. Anti-PRPSAP2 pAb (GTX118643) is tested in Human, Rat samples. 100% Ab-Assurance.
An intricate system of interrelated control mechanisms regulate biochemical reaction sequences. Metabolic pathways are controlled not only by specific activity and inherent kinetic properties of...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
How is PRPP synthetase-associated protein of 39 kDa abbreviated? PAP39 stands for PRPP synthetase-associated protein of 39 kDa. PAP39 is defined as PRPP synthetase-associated protein of 39 kDa rarely.
1P19: Interactions at the dimer interface influence the relative efficiencies for purine nucleotide synthesis and pyrophosphorolysis in a phosphoribosyltransferase.
List of all the English words with 30 letters containing letter B. amidophosphoribosyltransferase, dimethylacetylenedicarboxylate, methylenedioxybenzylpiperazine
1. The formation of adenosine 5-phosphate, guanosine 5-phosphate and inosine 5-phosphate from [8-(14)C]adenine, [8-(14)C]guanine and [8-(14)C]hypoxanthine respectively in the presence of 5-phosphoribosyl pyrophosphate and an extract from Ehrlich ascites-tumour cells was assayed by a method involving liquid-scintillation counting of the radioactive nucleotides on diethylaminoethylcellulose paper. The results obtained with guanine were confirmed by a spectrophotometric assay which was also used to assay the conversion of 6-mercaptopurine and 5-phosphoribosyl pyrophosphate into 6-thioinosine 5-phosphate in the presence of 6-mercaptopurine phosphoribosyltransferase from these cells. 2. At pH 7.8 and 25 degrees the Michaelis constants for adenine, guanine and hypoxanthine were 0.9 mum, 2.9 mum and 11.0 mum in the assay with radioactive purines; the Michaelis constant for guanine in the spectrophotometric assay was 2.6 mum. At pH 7.9 the Michaelis constant for 6-mercaptopurine was 10.9 mum. 3. 25 mum-6
During intense exercise a fraction of the ATP pool in human skeletal muscle is degraded to inosine-5-monophosphate (IMP). While most IMP is retained in the cell for reamination to AMP at rest, a significant fraction of IMP is further degraded to inosine and hypoxanthine and enter the bloodstream lowering the adenine nucleotide pool. Lost nucleotides must be restored via the purine salvage pathway or the de novo pathway of adenine nucleotide metabolism. The limiting step in nucleotide synthesis de novo is the availability of phosphoribosyl pyrophosphate (PRPP), which is formed from ribose-5-phosphate. The level of ribose in the muscle is limited; thus an increased availability of ribose may enhance the formation of PRPP and the rate of synthesis of adenine nucleotides. The aim of the present study was to assess the effect of oral intake of ribose after frequent, high-intensity training on adenine nucleotide resynthesis. Such information will not only be useful for people performing regular ...
The pentose phosphate pathway is a process of glucose turnover that produces NADPH as reducing equivalents and pentoses as essential parts of nucleotides. There are two different phases in the pathway. One is irreversible oxidative phase in which glucose-6P is converted to ribulose-5P by oxidative decarboxylation, and NADPH is generated [MD:M00006]. The other is reversible non-oxidative phase in which phosphorylated sugars are interconverted to generate xylulose-5P, ribulose-5P, and ribose-5P [MD:M00007]. Phosphoribosyl pyrophosphate (PRPP) formed from ribose-5P [MD:M00005] is an activated compound used in the biosynthesis of histidine and purine/pyrimidine nucleotides. This pathway map also shows the Entner-Doudoroff pathway where 6-P-gluconate is dehydrated and then cleaved into pyruvate and glyceraldehyde-3P [MD:M00008 ...
TY - JOUR. T1 - Motional dynamics of the catalytic loop in OMP synthase. AU - Wang, Gary P.. AU - Cahill, Sean M.. AU - Liu, Xiaohong. AU - Girvin, Mark E.. AU - Grubmeyer, Charles. PY - 1999/1/5. Y1 - 1999/1/5. N2 - In de novo pyrimidine biosynthesis, orotate phosphoribosyltransferase catalyzes the formation of orotidine 5-monophosphate (OMP) from orotic acid and α-D-5-phosphoribosyl-1-pyrophosphate (PRPP). The known three- dimensional structure of the dimeric enzyme from Salmonella typhimurium is similar to that of other Type I phosphoribosyltransferases (nucleotide synthases) with a solvent-exposed active site atop a Rossman-type nucleotide binding fold. The three-dimensional structure of an enzyme - inhibitor complex [Henriksen et al. (1996) Biochemistry 35, 3803-3809] indicates that of the two identical solvent-exposed loops can descend to cover the active site of the adjacent subunit of the dimeric enzyme. Catalytically essential residues are known to reside on this loop. In the present ...
Phosphoribosyl-pyrophosphate synthetase (Prs) catalyses the synthesis of phosphoribosyl pyrophosphate (PRPP), an intermediate in nucleotide metabolism and the biosynthesis of the amino acids histidine and tryptophan. The Saccharomyces cerevisiae genome contains a family of five PRS genes, PRS1-PRS5. Using anti-peptide antisera directed against two different epitopes of Prs1p it was shown that Prs1p localizes to granular cytoplasmic structures. This localization was confirmed by living cell microscopy of strains expressing a functional green fluorescent protein (GFP)-tagged Prs1p. Analysis of Prs1p distribution in conditional secretory-deficient (sec) mutants suggested that the observed distribution of Prs1p is independent of the secretory pathway. Electron microscopy revealed that plasma membrane invaginations and accumulation of cytoplasmic vesicles were more frequent in strains which lack some of the PRS genes than in the wild-type. The fact that Δprs1 and Δprs3 are hypersensitive to caffeine and
The purinosome is a putative multi-enzyme complex that carries out de novo purine biosynthesis within the cell. It is postulated to include all six of the human enzymes identified as direct participants in this ten-step biosynthetic pathway converting phosphoribosyl pyrophosphate to inosine monophosphate: The enzymes of the multi-step de novo purine biosynthesis pathway have been postulated to form a multi-enzyme complex to facilitate substrate channeling between each enzyme of the pathway. Slight variations of the pathway exists between phyla; however, there are 13 enzymes that can be considered part of this biosynthetic pathway. Several individual enzymatic functions have consolidated onto single bifunctional or trifunctional polypeptide chains in higher organisms, suggesting stable physical interactions exist between enzymes. The functional consolidation of steps 2,3, and 5 of the pathway into a single enzyme in higher organisms such as humans suggests physical local proximity of the enzyme ...
Based on the results of these studies we propose that accelerated purine nucleotide synthesis and uric acid overproduction in our patient resulted from PRS superactivity due to a point mutation in PRPS1, a T-for-A substitution at nucleotide 578 in the PRPS1 coding region. This mutation has 2 distinct functional effects on the activity of the PRS1 isoform thus encoded. First, as is the case in most of the point mutations encountered to date in affected male patients (6), the mutant PRS1 in our patient appears to be more labile than its normal counterpart, resulting in reduced concentrations and activities of the mutant isoform in the patients cells and accounting for the subnormal maximum PRS activities measured in her cell extracts at maximally activating Pi concentrations. Second, the mutation in the patients PRS1 imparts altered allosteric regulatory properties on the activity of the isoform. These altered properties include increased responsiveness of enzyme activity to activation by Pi ...
The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011 ...
Mycobacteria tuberculosis (Mtb), the causative agent of tuberculosis, is responsible for more death in the world today than any other bacteria. As part of the Tuberculosis Structural Genomics Consortium (TBSGC), our research group previously determined the structure of anthranilate phosphoribosyl transferase (AnPRT) from Mtb. AnPRT is the second enzyme in the tryptophan biosynthetic pathway and was identified as a potential drug target through gene knockout experiments, which resulted in a strain of Mtb that was essentially avirulent even in immunodeficient mice. AnPRT catalyses a reaction between anthranilate and phosphoribosylpyrophosphate (PRPP), and the crystal structure of Mtb-AnPRT was originally determined with and without PRPP (PDB ID: 1ZVW and 2BPQ, respectively). In silico docking was used to predict the binding motif of anthranilate, the second substrate, surprisingly predicted two sites despite a 1:1 reaction ratio with PRPP. Previously, 165 compounds were screened for inhibitory ...
4U28: Co-occurrence of analogous enzymes determines evolution of a novel ( beta alpha )8-isomerase sub-family after non-conserved mutations in flexible loop.
The pentose phosphate pathway is a process of glucose turnover that produces NADPH as reducing equivalents and pentoses as essential parts of nucleotides. There are two different phases in the pathway. One is irreversible oxidative phase in which glucose-6P is converted to ribulose-5P by oxidative decarboxylation, and NADPH is generated [MD:M00006]. The other is reversible non-oxidative phase in which phosphorylated sugars are interconverted to generate xylulose-5P, ribulose-5P, and ribose-5P [MD:M00007]. Phosphoribosyl pyrophosphate (PRPP) formed from ribose-5P [MD:M00005] is an activated compound used in the biosynthesis of histidine and purine/pyrimidine nucleotides. This pathway map also shows the Entner-Doudoroff pathway where 6-P-gluconate is dehydrated and then cleaved into pyruvate and glyceraldehyde-3P [MD:M00008 ...
human lymphoblast culture - posted in General Lab Techniques: Hi; I am new to research world and would like to learn more about human lymphoblasts culture techniques, and general rules used in tissue culture. Can someone direct me to good book / articles from where to start? I found many books, but I do not know where to start - I have bachelor in biology and psychology and currently taking some graduate classes but not directly related to research and I just have a chance to work in a l...
TY - JOUR. T1 - Comprehensive X-ray structural studies of the quinolinate phosphoribosyl transferase (BNA6) from Saccharomyces cerevisiae. AU - Di Luccio, Eric. AU - Wilson, David K.. PY - 2008/4/1. Y1 - 2008/4/1. N2 - Quinolinic acid phosphoribosyl transferase (QAPRTase, EC 2.4.2.19) is a 32 kDa enzyme encoded by the BNA6 gene in yeast and catalyzes the formation of nicotinate mononucleotide from quinolinate and 5-phosphoribosyl-1-pyrophosphate (PRPP). QAPRTase plays a key role in the tryptophan degradation pathway via kynurenine, leading to the de novo biosynthesis of NAD+ and clearing the neurotoxin quinolinate. To improve our understanding of the specificity of the eukaryotic enzyme and the course of events associated with catalysis, we have determined the crystal structures of the apo and singly bound forms with the substrates quinolinate and PRPP. This reveals that the enzyme folds in a manner similar to that of various prokaryotic forms which are ∼30% identical in sequence. In addition, ...
Incubation of human erythrocytes in medium containing inosine (10 mM), pyruvate (10 mM), phosphate (50 mM) and NaCl (75 mM) at pH 6.6 leads to a more than 1000-fold increase in the concentration of 5-phosphoribosyl 1-pyrophosphate (PRPP), as identified and quantified by 31P-n.m.r. spectroscopy. The accumulation is highly pH-dependent, with a maximum at extracellular pH 6.60, and the maximum value of 1.3-1.6 mmol/l of erythrocytes is attained within 1 h at 37 degrees C. PRPP was accumulated despite high concentrations of 2,3-bisphosphoglycerate (2,3-BPG), an inhibitor of PRPP synthetase. The concentration of PRPP correlated with the intracellular concentration of inorganic phosphate (Pi). Substitution of either adenosine or adenosine plus inosine for inosine in the medium did not lead to 31P-n.m.r.-detectable accumulation of PRPP. These results show that neither 2,3-BPG nor PRPP itself inhibits the synthesis of PRPP in the human erythrocyte. Adenosine, however, prevents the inosine-stimulated ...
Looking for the definition of HGPRT? Find out what is the full meaning of HGPRT on Abbreviations.com! Hypoxanthin guanine phosphoribosyl tranferase is one option -- get in to view more @ The Webs largest and most authoritative acronyms and abbreviations resource.
MalaCards based summary : Dfnx1 Nonsyndromic Hearing Loss and Deafness, also known as dfn2 nonsyndromic hearing loss deafness, is related to deafness, x-linked 1 and branchiootic syndrome 1. An important gene associated with Dfnx1 Nonsyndromic Hearing Loss and Deafness is PRPS1 (Phosphoribosyl Pyrophosphate Synthetase 1 ...
Abstract: Allosteric modulation of catalysis is a common regulatory strategy of flux-controlling biosynthetic enzymes. The enzyme ATP phosphoribosyltransferase (ATPPRT) catalyses the first reaction in histidine biosynthesis, the magnesium-dependent condensation of ATP and 5-phospho--D-ribosyl-1-pyrophosphate (PRPP) to generate N1-(5-phospho--D-ribosyl)-ATP (PRATP) and pyrophosphate (PPi). ATPPRT is allosterically inhibited by the final product of the pathway, histidine. Hetero-octameric ATPPRT consists of four catalytic subunits (HisGS) and four regulatory subunits (HisZ) engaged in intricate catalytic regulation. HisZ enhances HisGS catalysis in the absence of histidine while mediating allosteric inhibition in its presence. Here we report HisGS structures for the apoenzyme and complexes with substrates (PRPP, PRPP-ATP, PRPP-ADP), product (PRATP), and inhibitor (AMP), along with ATPPRT holoenzyme structures in complexes with substrates (PRPP, PRPP-ATP, PRPP-ADP) and product (PRATP). These ...
Hipoksantin fosforiboziltransferaza (EC 2.4.2.8, IMP pirofosforilaza, transfosforibozidaza, hipoksantin---guanin fosforiboziltransferaza, guaninska fosforiboziltransferaza, GPRT, HPRT, guanozin 5-fosfat pirofosforilaza, IMP-GMP pirofosforilaza, HGPRTase, 6-hidroksipurin fosforiboziltransferaza, 6-merkaptopurin fosforiboziltransferaza, GMP pirofosforilaza, guanin-hipoksantin fosforiboziltransferaza, guanozin fosforiboziltransferaza, guanilat pirofosforilaza, guanilinska pirofosforilaza, inozinatna pirofosforilaza, inozin 5-fosfatna pirofosforilaza, inozinsko kiselinska pirofosforilaza, inozinska pirofosforilaza, 6-merkaptopurinska fosforiboziltransferaza, purin-6-tiolna fosforiboziltransferaza) je enzim sa sistematskim imenom IMP:difosfat fosfo-D-riboziltransferaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju ...
... is an integrated process in all human cells that is intimately linked with the pathways of nucleotide synthesis and salvage
Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5 triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3,5-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity ...
This enzyme belongs to the family of transferases that transfer one-carbon groups, specifically the hydroxymethyl-, formyl- and related transferases. The systematic name of this enzyme class is 10-formyltetrahydrofolate:5-phosphoribosyl-5-amino-4-imidazole-carb oxamide N-formyltransferase. Other names in common use include: ...
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CVSROOT: /cvs/purrs Module name: purrs Changes by: zolo at cs.unipr.it 2003-10-22 16:31:37 Modified files: src : Recurrence.inlines.hh Log message: Added the infinite order recurrences in the method `Recurrence::verify_exact_solution(). Patches: http://www.cs.unipr.it/cgi-bin/cvsweb.cgi/purrs/src/Recurrence.inlines.hh.diff?cvsroot=purrs&r1=1.72&r2=1.73 ...
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nucleotide pyrophosphate transferase: transfers the beta, gamma pyrophosphate group from ATP to 3-hydroxy group of ribonucleotides, oligonucleotides or tRNA
CAS:7320-34-5 Synonyms: Diphosphoric acid, tetrapotassium salt; Potassium Diphosphate; Tetra-Potassium Pyrophosphate; TKPP; Tetrapotassium pyrophosphate; tetrapotassium diphosphate; diphosphoric acid, monopotassium salt Product: Potassium...
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The Golm Metabolome Database (GMD) facilitates the search for and dissemination of mass spectra from biologically active metabolites quantified using GC-MS.
CVSROOT: /cvs/purrs Module name: purrs Changes by: zolo at cs.unipr.it 2003-12-23 18:24:57 Modified files: src : Recurrence.defs.hh Recurrence.cc Log message: Renamed `verify_new_method_exp_poly() in `verify_new_method_const_coeff(). Added the method `verify_new_method_var_coeff() (it is still commented because not finished). Patches: http://www.cs.unipr.it/cgi-bin/cvsweb.cgi/purrs/src/Recurrence.defs.hh.diff?cvsroot=purrs&r1=1.167&r2=1.168 http://www.cs.unipr.it/cgi-bin/cvsweb.cgi/purrs/src/Recurrence.cc.diff?cvsroot=purrs&r1=1.159&r2=1.160 ...
How is De Novo Purine Synthesis abbreviated? DNPS stands for De Novo Purine Synthesis. DNPS is defined as De Novo Purine Synthesis somewhat frequently.
Affinity-purified antibodies that recognize Thymidylate Synthase (TS), Dihydropyrimidine Dehydrogenase (DPD), and Orotate Phosphoribosyltransferase (OPRT) that can be used for Western blot or immunohistochemistry
Affinity-purified antibodies that recognize Thymidylate Synthase (TS), Dihydropyrimidine Dehydrogenase (DPD), and Orotate Phosphoribosyltransferase (OPRT) that can be used for Western blot or immunohistochemistry