Gentaur molecular products has all kinds of products like :search , AbD \ MOUSE ANTI HUMAN NEURON SPECIFIC ENOLASE, Product Type Monoclonal Antibody, Specificity NEURON SPECIFIC ENOLASE, Target Species Human, Host Mouse, Format Purified, Isotypes IgG2a, Applications \ 0300-0104 for more molecular products just contact us
Enolase Activity Assay Kit is a quantitative colormetric/fluorometric determination of Enolase Activity. (KA3839) - Products - Abnova
Three enolase isozymes are known in mammals, each with a characteristic tissue distribution: alpha, beta, and gamma enolase. The ENO2 gene encodes enolase 2 (gamma, neuronal) and is expressed in mature neurons and cells of neuronal origin. It is also known as neural enolase, neuron-specific enolase (NSE), 2-phospho-D-glycerate hydrolyase, gamma-enolase, neuron-specific gamma enolase, and enolase 2. NSE exhibits neurotrophic and neuroprotective properties in central nervous system (CNS) neurons. The calcium-regulated binding of NSE to cultured neocortical neurons promotes cell survival. NSE was detected in small-cell carcinomas of neuroendocrine origin, and has been proposed as a marker for lung cancer patients.. ...
Three enolase isozymes are known in mammals, each with a characteristic tissue distribution: alpha, beta, and gamma enolase. The ENO2 gene encodes enolase 2 (gamma, neuronal) and is expressed in mature neurons and cells of neuronal origin. It is also known as neural enolase, neuron-specific enolase (NSE), 2-phospho-D-glycerate hydrolyase, gamma-enolase, neuron-specific gamma enolase, and enolase 2. NSE exhibits neurotrophic and neuroprotective properties in central nervous system (CNS) neurons. The calcium-regulated binding of NSE to cultured neocortical neurons promotes cell survival. NSE was detected in small-cell carcinomas of neuroendocrine origin, and has been proposed as a marker for lung cancer patients.. ...
In higher vertebrates, the glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase; EC 4.2.1.11) is active as a dimer formed from three different subunits, α, β and γ, encoded by separate genes. Th
NSE, or Enolase 2 (gamma, neuronal), is a human gene encoded by ENO2. It may also be known as: Gamma-enolase; Enolase 2; Neural enolase; Neuron-specific enolase; Gamma-enolase ; HEL-S-279. The encoded protein is an enzyme with an amino acid length of 434 and a mass of 47.3 kDa. Other isoforms may exist. NSE is a member of the Enolase family. Homologs of this gene exist in other organisms, including: Mouse, Rat.. Abbkine NSE Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous NSE proteins. Its suitable to be applied in WB, IHC and IF. Its verified to react with human, mouse and rat. The concentration of this monoclonal antibody is 1mg/ml.. Our group purchased Abbkine NSE monoclonal antibody for detecting NSE proteins in paraffin-embedded Human-colon tissue sections. Our sample were incubated with Abbkine NSE monoclonal antibody (diluted at 1:200) overnight at +4°C. The sensitivity of the antibody was ...
Tawk RG, Grewal SS, Heckman MG, Rawal B, Miller DA, Edmonston D, Ferguson JL, Navarro R, Ng L, Brown BL, Meschia JF, Freeman WD. The Relationship Between Serum Neuron-Specific Enolase Levels and Severity of Bleeding and Functional Outcomes in Patients With Nontraumatic Subarachnoid Hemorrhage. Neurosurgery. 2016 Apr; 78: (4)487-91 ...
Buy goat anti-human Neuron Specific Enolase (NSE) polyclonal antibodies for research, life science and manufacturing applications!
TY - JOUR. T1 - Discovery of function in the enolase superfamily. T2 - D-mannonate and d-gluconate dehydratases in the d-mannonate dehydratase subgroup. AU - Wichelecki, Daniel J.. AU - Balthazor, Bryan M.. AU - Chau, Anthony C.. AU - Vetting, Matthew W.. AU - Fedorov, Alexander A.. AU - Fedorov, Elena V.. AU - Lukk, Tiit. AU - Patskovsky, Yury V.. AU - Stead, Mark B.. AU - Hillerich, Brandan S.. AU - Seidel, Ronald D.. AU - Almo, Steven C.. AU - Gerlt, John A.. PY - 2014/4/29. Y1 - 2014/4/29. N2 - The continued increase in the size of the protein sequence databases as a result of advances in genome sequencing technology is overwhelming the ability to perform experimental characterization of function. Consequently, functions are assigned to the vast majority of proteins via automated, homology-based methods, with the result that as many as 50% are incorrectly annotated or unannotated (Schnoes et al. PLoS Comput. Biol. 2009, 5 (12), e1000605). This manuscript describes a study of the d-mannonate ...
More than a decade after the genomic age, full genome sequencing is cost-effective and fast, allowing for the deposit of an ever increasing number of DNA sequences. New fields have arisen from this availability of genomic information, and the way we think about biochemistry and enzymology has been transformed. Unfortunately, there is no robust method for accurately determining the functions of enzymes encoded by these sequences that matches the speed in which genomes are deposited into public databases. Functional assignment of enzymes remains of utmost importance in understanding microbial metabolism and has applications in agriculture by examining bacterial plant pathogen metabolism and additionally in human health by providing metabolic context to the human gut microbiome. To aid in the functional identification of proteins, enzymes can be grouped into superfamilies which share common structural motifs as well as mechanistic features. To this end, the enolase superfamily is an excellent model ...
Abstract: Adrenocortical cancer (ACC) is a rare and aggressive endocrine tumor. The patient presented with a large retroperitoneum tumor and lung metastases. Removal of the adrenocortical tumor with part of the transverse colon and tail of the pancreas, spleen and kidney was successfully performed following chemotherapy. Levels of serum neuron-specific enolase (NSE) were found to be markedly high before surgery and may be clinically useful markers for monitoring tumor status during management. Immunohistochemical studies showed that the cancer cells were positive for NSE and overexpression of p53. We identified a novel germ line variant of the 177 mutant (Pro to Arg; P177R) of p53 by genomic sequencing. The genetic and biochemical data presented in this case confirm the importance of screening for p53 status in ACC with inherited cancer syndrome.. ...
The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11) exists as several dimeric isoenzymes (αα, αβ, αγ, ββ and γγ) composed of three distinct subunits α, β and γ. The γ-unit is found either in a homologous γγ- or in a heterologous α, γ-isoenzyme and is known as neuron-specific enolase (NSE). The monoclonal antibodies used in the CanAg NSE EIA bind to the γ-subunit of the enzyme and thereby detects both the γγ- and the αγ-forms (1, 2). The NSE levels are low in healthy subjects and subjects with benign diseases. Elevated levels are commonly found in patients with malignant tumours with neuroendocrine differentiation, especially small cell lung cancer (SCLC) (3) and neuroblastoma (4 ...
TY - JOUR. T1 - Streptococcus sanguinis and the sera of patients with Behçets disease stimulate membrane expression of α-enolase in human dermal microvascular endothelial cells. AU - Cho, Suhyun. AU - Zheng, Zhenlong. AU - Cho, Sung Bin. AU - Choi, Min Ju. AU - Lee, Kwang Hoon. AU - Bang, Dongsik. PY - 2013/4/1. Y1 - 2013/4/1. N2 - The glycolytic enzyme α-enolase is a plasminogen-binding protein that is generally found in the cytosolic compartment. However, α-enolase can also be expressed on cell surfaces following an inflammatory stimulus via an unknown mechanism. We investigated the effects of Streptococcus sanguinis (S. sanguinis) and the sera of patients with Behçets disease (BD) on the expression and distribution of α-enolase in human dermal microvascular endothelial cells (HDMECs). HDMECs were stimulated with cultured S. sanguinis and the sera of active BD patients. HDMECs incubated for 6, 12 or 24 h were harvested, and the membrane and cytoplasmic fractions of proteins were ...
The effect of hydroalcoholic extract of artemisia. Hu y, et al. Immunisation with the glycolytic enzyme enolase confers powerful safety in opposition to candida albicans infection in mice. Vaccine. 2011;29. Systemic contamination following intravenous inoculation of. Systemic contamination following intravenous inoculation of mice with candida albicans int1 precipitated mortality or the route of progressive infection […]. Continue reading ...
Rika Susanti. Blunt brain injury is the highest cause of death in crime. Many efforts to determining cause of death, besides autopsy, have been done. CKBB, NSE, and S100B levels is some of biomarkers that found in human getting blunt brain injury. Alternative of determining cause of death, besides autopsy, is focused to these biomarkers. Studys purpose is to analyze CKBB, NSE, and S100B levels in cerebrospinal fluid and serum to determining cause of death and time of death. Moreover, this study describes about histopathology description of brain tissue.. This is experimental study with post test only group design, comparing handling of death caused because of blunt brain injury to ketamine acute poisoning. Total of handling groups are 8. Each group has 6 mature Rattusnovergicus Sprague Dawley. CKBB, NSE, and S100B levels were examined by sandwich ELISA after 0 hour, 1 hour, 2 hours and 3 hours post mortem. This study examined histopathologyof brain tissue usingHematoxylin Eosin staining. It ...
Allow specimen to clot completely at room temperature. Separate serum from cells ASAP. Serum should be separated from cells immediately to avoid release of NSE from blood cells ...
Mandelate racemase (MR) from Pseudomonas putida catalyzes the Mg(2+)-dependent, 1,1-proton transfer reaction that racemizes (R)- and (S)-mandelate. MR shares a partial reaction (i.e., the metal ion-assisted, Brønsted base-catalyzed proton abstraction of the α-proton of carboxylic acid substrates) and structural features ((β/α)7β-barrel and N-terminal α + β capping domains) with a vast group of homologous, yet functionally diverse, enzymes in the enolase superfamily. Mechanistic and structural studies have developed this enzyme into a paradigm for understanding how enzymes such as those of the enolase superfamily overcome kinetic and thermodynamic barriers to catalyze the abstraction of an α-proton from a carbon acid substrate with a relatively high pKa value ...
Cytosolic levels of neuron-specific enolase in squamous cell carcinomas of the lung. - A Ruibal, M I Nuñez, J Rodríguez, L Jiménez, M C del Rio, J Zapatero
Immunohistochemistry and EM can provide further supportive evidence of GANTs [6,9-11,13]. The tumors are stained consistently positive for vimentin and neuron-specific enolase. Some may also be stained positive for synaptophysin, S-100, neurofilaments, vasoactive intestinal peptides, and chromogranin. Its stained negative for cytokeratin, muscle specific actin, CD34, desmin, serotonin, and glial fibrillary acid protein. The tumor we report was composed of plump eosinophilic cytoplasm and was stained positive for vimentin, c-kit, neuron specific enolase, S-100, neurofilaments, synaptophysin, and smooth muscle actin. Diagnostic ultrastructural features include neuro-scretory granules, neurotubles, skeinoid fibers and the interdigitating complex cellular processes joined by rudimentary cell junctions [10-13]. Initially, GANT was regarded as malignant with poor prognosis [4]. This view was later challenged by Lee et al. [12], who suggested GANT was not always malignant. Histology wont give easy ...
Enolases are enzymes which catalyze the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse…
Gamma-enolase, known also as neuron-specific enolase (NSE), is an enzyme of the glycolytic pathway, which is expressed predominantly in neurons and cells of the neuroendocrine system. As a tumour marker it is used in diagnosis and prognosis of cancer; however, the mechanisms enrolling it in malignan ...
Glucose is unstable in whole blood (1). Fluoride-containing tubes are used to reduce glycolysis, especially when very accurate glucose results are required. Complete inhibition of glycolysis by fluoride can take as long as 4 h, during which time glucose can fall as much as 10 mg/dL (0.6 mmol/L) at room temperature (1), even in samples with normal blood cell counts. This is because fluoride inhibits the enzyme enolase, which acts late in the Embden-Meyerhof pathway, thus allowing the "breakdown" of glucose to intermediate molecules earlier in the glycolytic cycle (2). Is there a better fluoride formulation available that can prevent this problem?. Inclusion of citrate buffer and EDTA in a fluoride tube produces immediate inhibition of glycolysis, with no loss of glucose prior to centrifugation for at least 8 h at 77°F (25°C) (3). Even after 24 h, the loss of glucose is only 1.3 mg/dL (0.07 mmol/L). Hexokinase, which converts glucose to glucose-6-phosphate in the initial step of the glycolytic ...
1ECQ: Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glucarate dehydratase from Escherichia coli.
1EC7: Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glucarate dehydratase from Escherichia coli.
Autoimmunity to retinal, neuronal, and axonal antigens may play a role in inducing cellular degeneration. The specific AAbs against retinal antigens are most commonly associated with CAR and AR but their pathogenic effects were not fully established [14]. The current studies showed that serum anti-optic nerve AAbs are also present in more than 50% patients tested in patients with either CAR or AR. The major optic nerve antigenic targets for patients AAbs described in these studies can be divided into four categories as follows: (1) classical glycolytic enzymes involved in energy production, including α and γ enolases, glyceraldehyde 3-phosphate dehydrogenase, which also reacted with retinal antigens; (2) neuronal-specific myelin proteins; (3) CRMP5; (4) AQP4. Also other antibodies were present but their antigens have not yet been discovered and are under investigation. The newly identified AAbs labeled optic nerve components such as axons, astrocytes, and oligodendrocytes. Although their role ...
Researchers have described a new selective target in muscle regeneration. This is the association of alpha-enolase protein and plasmin, findings which could be used to develop new treatments to regenerate muscular injuries or dystrophies. |!-- --| read more via Science 2.0 Read More...
|b||i|Background/Aims:|/i||/b| Post-operative cognitive decline is frequent in older individuals following major surgery; however, biomarkers of this decline are less clearly d
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
GF ID Nse5 #=GF AC PF08691.9 #=GF DE DNA repair proteins Nse5 and Nse6 #=GF AU Mistry J, Wood V #=GF SE manual #=GF GA 25.00 25.00; #=GF TC 28.10 26.30; #=GF NC 24.40 21.70; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq #=GF TP Family #=GF RN [1] #=GF RM 16478984 #=GF RT The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 #=GF RT complex. #=GF RA Pebernard S, Wohlschlegel J, McDonald WH, Yates JR 3rd, Boddy #=GF RA MN; #=GF RL Mol Cell Biol. 2006;26:1617-1630. #=GF DR INTERPRO; IPR014803; #=GF CC Nse5 and Nse6 are non essential nuclear proteins that are #=GF CC critical for chromosome segregation in fission yeast [1]. Nse5 #=GF CC forms a dimer with Nse6 and facilitates DNA repair as part of #=GF CC the Smc5-Smc6 holocomplex. #=GF SQ 54 #=GS J7S707_KAZNA/90-379 AC J7S707.1 #=GS K0K7N9_WICCF/217-302 AC K0K7N9.1 #=GS J8LPK5_SACAR/33-467 AC J8LPK5.1 #=GS Q756F5_ASHGO/1-501 AC Q756F5.2 #=GS Q6FSX3_CANGA/1-531 AC Q6FSX3.1 #=GS ...
Highly Updated And Relevant NSE4-5.4 Questions And Answers For NSE4 NSE4-5.4 Exam. Interactive Testking Fortinet NSE4-5.4 Exam Engine With Various Practice Exam Options. NSE4-5.4 Test King.
Det er mange arter av busker som er eviggrønne og grunnen til dette er at de kan tilpasse seg lav matforsyning. Det er slike anlegg for alle typer jord så...
Read "Plant enolase: gene structure, expression, and evolution." on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
hivi ni vyakula ambavyo haviwezi kurudi shambani na kuota au vilikua na uwezo wa kuota lakini vimepikwa hivyo havina uwezo tena wa kuota shambani, mfano wa vyakula hivyo ni nyama, dagaa, samaki, kuku, chips, mayai, viazi vilivyopikwa, sukari, soda, pombe, mihogo iliyopikwa, mikate, keki, maandazi, chapati, wali, ugali na kadhalika ...
NSE兔多克隆抗体(ab834)可与牛, 人样本反应并经IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
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Muligheden opstår pludselig, da Ki-woo på sin vens anbefaling får et job som engelsktutor for den kønne, unge Da-hye, hvis hovedrige far og neurotiske mor betaler godt for lektiehjælpen ...
TY - JOUR. T1 - Immunohistochemical detection of carcinoembryonic antigen and P-glycoprotein in small cell lung cancer at diagnosis and relapse, with special reference to the tissue expression of CEA and response to chemotherapy. AU - Ohnoshi, T.. AU - Ueoka, H.. AU - Segawa, Y.. AU - Kiura, Katsuyuki. AU - Tabata, Masahiro. AU - Shibayama, T.. AU - Maeda, T.. AU - Miyatake, M.. AU - Takigawa, N.. AU - Kimura, I.. PY - 1992. Y1 - 1992. N2 - Small cell lung cancer (SCLC) is one of the most sensitive tumors to drug therapy; however, the majority of patients eventually relapse within a few years. Emergence of drug resistance is thought to play a major role in the dismal course of this disease. However, the mechanism of drug resistance in SCLC still remains obscure. Based on the clinical observation that a significant proportion of patients with relapsing tumor show an elevated serum carcinoembryonic antigen (CEA) concentration while serum neuron-specific enolase (NSE) concentration remains normal, ...
Enolase is the eighth enzyme in the glycolytic pathway, a reaction that generates ATP from phosphoenol pyruvate in cytosolic compartments. Enolase is essential, especially for organisms devoid of the Krebs cycle that depend solely on glycolysis for energy. Interestingly, enolase appears to serve a separate function in some organisms, in that it is also exported to the cell surface via a poorly understood mechanism. In these organisms, surface enolase assists in the invasion of their host cells by binding plasminogen, an abundant plasma protease precursor. Binding is mediated by the interaction between a lysine motif of enolase with Kringle domains of plasminogen. The bound plasminogen is then cleaved by specific proteases to generate active plasmin. Plasmin is a potent serine protease that is thought to function in the degradation of the extracellular matrix surrounding the targeted host cell, thereby facilitating pathogen invasion. Recent work revealed that the malaria parasite Plasmodium ...
Enolase ELISA Kit is a solid-phase enzyme immunoassay for the quantitative detection of neuron-specific enolase. (KA4023) - Products - Abnova
Results The analysis of the protein profiles allowed us to find 26 spots with a different expression in FM respect to RA (p-value,0.05), 28 spots from the comparison of FM with migraine, and 32 in FM respect to healthy subjects. In particular, we found 7 spots differentially expressed exclusively in FM. Six spots were identified as serotransferrin and the other as alpha-enolase. Both serotransferrin and alpha-enolase increased in FM respect to all the control classes. Furthermore, the proteins differentially expressed in FM respect to the control classes, were functionally analyzed by using the Ingenuity Pathways Analysis software with the aim to determine the predominant canonical pathways and the interaction network involved. Serotransferrin is an iron binding transport protein, responsible for the transport of iron from sites of absorption to those of storage and utilization, and alpha-enolase is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various ...
Approximately 40% of patients show autoantibodies against a citrullinated epitope of α-enolase, the CEP-1 peptide, which are highly specific for the disease (97%). Interestingly, CEP-1 shows a remarkably high sequence homology to the corresponding peptide of the bacterial α-enolase of P. gingivalis. Therefore, human anti-CEP-1 antibodies are able to recognise and bind recombinant, in vitro-citrullinated P. gingivalis enolase. This has led to the hypothesis of molecular mimicry as a cause of RA: In order not to be identified as foreign by the immune system, P. gingivalis has an α-enolase which is similar to the human α-enolase. Due to the similarity, the bacterial PAD might citrullinate both the P. gingivalis enolase and the human enolase, which sensitive persons subsequently produce antibodies against. Alternatively, there is the possibility that the immune response is really primarily directed against the bacteria and their citrullinated proteins, amongst others α-enolase, whereby the ...
Usando sondas isotópicas, o mecanismo global proposto para converter o 2-PG en PEP é unha reacción de eliminación E1cb na que está implicado un intermediato carbanión.[7] O seguinte mecanismo detallado está baseado en estudos da estrutura cristalina e cinética.[3][8][9][10][11][12][13] Cando o substrato 2-fosfoglicerato se une á α-enolase, o seu grupo carboxilo coordínase con dous cofactores ións magnesio no sitio activo. Isto estabiliza a carga negativa no oxíxeno desprotonado á vez que incrementa a acidez do hidróxeno alfa. A Lys345 da enolase desprotona o hidróxeno alfa, e a carga negativa resultante é estabilizada por resonancia co oxíxeno do carboxilato e polos cofactores Mg2+. Despois da creación do intermediato carbanión, o OH de C3 é eliminado como auga coa axuda do Glu211, e fórmase o PEP. Adicionalmente, no encima teñen lugar cambios conformacionais que axudan á catálise. Na α-enolase humana, o substrato está rotado na súa posición ao unirse ao encima ...
import matplotlib.pyplot as plt import numpy as np class PID: An implementation of a PID control class for use in process control simulations. def __init__(self, name=None, SP=None, Kp=0.2, Ki=0, Kd=0, beta=0, gamma=0, MVrange=(0,100), DirectAction=False): self.name = name self.SP = SP self.Kp = Kp self.Ki = Ki self.Kd = Kd self.beta = beta self.gamma = gamma self.MVrange = MVrange self.DirectAction = DirectAction self._mode = inAuto self._log = [] self._errorP0 = 0 self._errorD0 = 0 self._errorD1 = 0 self._lastT = 0 self._currT = 0 def auto(self): Change to automatic control mode. self._mode = inAuto def manual(self): Change to manual control mode. In manual mode, the setpoint tracks the process variable to provide bumpless transfer on return to auto. self._mode = inManual def _logger(self,t,SP,PV,MV): self._log.append([t,SP,PV,MV]) def plot(self): Create historical plot of SP,PV, and MV using the controllers internal log file. dlog = np.asarray(self._log).T ...
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What I want to say is that the anomalously high chance that the \(126\GeV\) Higgs boson splits into two photons, \(h\to\gamma\gamma\), is no longer anomalously high. ATLAS and CMS used to suggest that the rate could be up to \(1.8\) times the Standard Model prediction. However, ATLAS updated the figure to something like \(1.5\pm 0.3\) (which is pretty much one) a week ago and today, CMS updated its results and the new value is even lower than expected, \(0.8\pm 0.3\). Calculate the ATLAS+CMS average and you will find out that the decay rate is what it should be within less than one sigma ...
NS-398 inhibits COX-2 enzyme activity in a concentration dependent manner, the IC50 being 3.8 μM, whereas NS-398 at 100μM has no effect on COX-1 activity[1]. At 10 μM, NS-398 treatment results in increased production of COX-2 and the pro-inflammatory cytokine. NS-398 (10 μM) induces apoptosis in LNCaP cells, but not in the more aggressive, androgen-unresponsive C4-2b cells. The C4-2b cells are observed to continue to proliferate when treated with NS-398 and continues to retain malignant phenotype characteristics. NS-398 treatment results in C4-2b cell differentiation into an unusual neuroendocrinelike cell. These neuroendocrine-like cells produces both epithelial (cytokeratin 18 and prostate specific antigen) and neuronal (neuron-specific enolase and chromogranin A) proteins. Furthermore, this C4-2b cellular response to NS-398 is mediated by NF-kB transcription factor activation. NS-398 induces NF-kB and down-regulates Ikβ-α protein expression in LNCaP C4-2b cells[2]. ...
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Complete information for ENO4 gene (Protein Coding), Enolase Family Member 4, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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