Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro (By similarity).

A wheat non specific phospholipid transfer protein has been isolated from wheat seeds and its amino acid sequence reveals that it is composed of 90 residues for a molecular weight of 9607. From the comparison of its sequence with those of the eight known proteins of the same family, hypotheses on the role of some conserved residues in the transfer activity can be made. The conformation of this protein has been studied by Raman and Fourier transform infrared spectroscopy and this is the first report on the structure of non specific plant phospholipid transfer proteins. As opposed to previous studies on the structure prediction from the amino acid sequence, the results obtained show that plant non specific phospholipid transfer proteins are not almost entirely composed of beta-sheets. Instead, infrared results show that the wheat protein contains 41% alpha-helix and 19% beta-sheet structures, while 40% of the conformation is undefined or composed of turns. Raman spectroscopy shows that three disulfide

TY - JOUR. T1 - Phospholipid transfer protein-deficient mice absorb less cholesterol. AU - Liu, Ruijie. AU - Iqbal, Jahangir. AU - Yeang, Calvin. AU - Wang, David Q.H.. AU - Hussain, M. Mahmood. AU - Jiang, Xian Cheng. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2007/9. Y1 - 2007/9. N2 - OBJECTIVE - Phospholipid transfer protein (PLTP) plays an important role in lipoprotein metabolism and atherosclerosis. PLTP gene knockout (KO) mice show significant reduction of plasma cholesterol levels. Because small intestine is one of the major tissue expressing PLTP, we hypothesize that PLTP deficient small intestine absorbs less cholesterol, thus contributing to the diminishing of cholesterol levels in the plasma. METHODS AND RESULTS - We used dual-labeled cholesterol/sitostanol feeding approach to study cholesterol absorption in PLTP KO and WT mice. We found that PLTP KO mice absorb significant less cholesterol than WT mice. Primary enterocytes isolated from PLTP KO ...

Human phospholipid transfer protein (PLTP) mediates the transfer of lipids among atheroprotective high-density lipoproteins (HDL) and atherogenic low-density lipoproteins (LDL) by an unknown mechanism. Delineating this mechanism would be an important step toward the understanding and regulation of PLTP for treating cardiovascular diseases, hypoalphalipoproteinemia and hyperalphalipoproteinemia. Using electron microscopy, negative-staining, and single-particle image processing, we discovered that PLTP penetrates each class of HDL, LDL and liposome independently, and also bridges a ternary complex with one of its distal end-domains penetrating into HDL and another distal domain interacting with LDL. These new insights into PLTP interaction with lipoproteins and liposomes provide a molecular basis for analyzing PLTP-dependent lipid transfer between lipoprotein particles. ...

Order Aspergillus clavatus Phosphatidylinositol transfer protein sfh5 sfh5 -Mammalian Cell 01022755170 at Gentaur Aspergillus clavatus Phosphatidylinositol transfer protein sfh5 (sfh5) Mammalian

The ORPs (oxysterol-binding-protein-related proteins) constitute an enigmatic family of intracellular lipid receptors that are related through a shared lipid binding domain. Emerging evidence suggests that ORPs relate lipid metabolism to membrane transport. Current data imply that the yeast ORP Kes1p is a negative regulator of Golgi-derived vesicular transport mediated by the essential phosphatidylinositol/phosphatidylcholine transfer protein Sec14p. Inactivation of Kes1p function allows restoration of growth and vesicular transport in cells lacking Sec14p function, and Kes1p function in this regard can be complemented by human ORP1S (ORP1 short). Recent studies have determined that Kes1p and ORP1S both bind phospholipids as ligands. To explore the function of distinct linear segments of ORP1S in phospholipid binding and vesicular transport regulation, we generated a series of 15 open reading frames coding for diagnostic regions within ORP1S. Purified versions of these ORP1S deletion proteins ...

Buy PLSCR4 elisa kit, Human Phospholipid scramblase 4 ELISA Kit-Q9NRQ2.2 (MBS283171) product datasheet at MyBioSource, ELISA Kits

Antibodies for proteins involved in phospholipid scramblase activity pathways, according to their Panther/Gene Ontology Classification

DESCRIPTION (provided by applicant): Molecular mechanisms utilized by axons to ensure proper wiring in the brain involve multiple ligand-receptor interactions, as extracellular guidance cues, such as netrins and RGMs (repulsive guidance molecule), are critical in the process of axon path-finding. Receptors for netrins involved in axon path-finding include DCC (deleted in colorectal cancer) and neogenin, a family of immunoglobin domain-containing proteins. Neogenin is also a receptor for RGMs. The long-term goal of our work is to elucidate intracellular signaling mechanisms initiated by DCC and neogenin engagement. In this regard, the PIs laboratory has identified binding partners for DCC and neogenin which are likely to participate in netrin-1/RGM-induced intracellular signaling, including PITPalpha (phosphatidylinositol transfer protein alpha), a protein essential for ),), ( phosphatidylinositol signaling, and myosin X (Myo X), an unconventional myosin implicated in cell adhesion and ...

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Shop Phospholipid scramblase ELISA Kit, Recombinant Protein and Phospholipid scramblase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.

Lipid droplet (LD) utilization is an important cellular activity that regulates energy balance and release of lipid second messengers. Because fatty acids exhibit both beneficial and toxic properties, their release from LDs must be controlled. Here we demonstrate that yeast Sfh3, an unusual Sec14-like phosphatidylinositol transfer protein, is an LD-associated protein that inhibits lipid mobilization from these particles. We further document a complex biochemical diversification of LDs during sporulation in which Sfh3 and select other LD proteins redistribute into discrete LD subpopulations. The data show that Sfh3 modulates the efficiency with which a neutral lipid hydrolase-rich LD subclass is consumed during biogenesis of specialized membrane envelopes that package replicated haploid meiotic genomes. These results present novel insights into the interface between phosphoinositide signaling and developmental regulation of LD metabolism and unveil meiosis-specific aspects of Sfh3 (and ...

TY - JOUR. T1 - The phosphatidylcholine transfer protein StarD7 is important for myogenic differentiation in mouse myoblast C2C12 cells and human primary skeletal myoblasts. AU - Horibata, Yasuhiro. AU - Mitsuhashi, Satomi. AU - Shimizu, Hiroaki. AU - Maejima, Sho. AU - Sakamoto, Hirotaka. AU - Aoyama, Chieko. AU - Ando, Hiromi. AU - Sugimoto, Hiroyuki. PY - 2020/12/1. Y1 - 2020/12/1. N2 - StarD7 is a phosphatidylcholine (PC)-specific lipid transfer protein essential for the maintenance of mitochondrial PC composition, morphogenesis, and respiration. Here, we studied the role of StarD7 in skeletal myoblast differentiation using mouse myoblast C2C12 cells and human primary myoblasts. Immunofluorescence and immuno-electron microscopy revealed that StarD7 was distributed in the cytosol, inner mitochondria space, and outer leaflet of the outer mitochondrial membrane in C2C12 cells. Unlike human kidney embryonic cell line HEK293 cells, the mitochondrial proteinase PARL was not involved in the ...

We have purified a 38 kDa protein from bovine brain which is cross-reactive with an affinity purified antibody against the 35 kDa phosphatidylino-sitol transfer protein from the same source. Controlled trypsinization of the 38 kDa protein yielded an immunoreactive protein of 35 kDa which displayed a 6-fold increase in phosphatidylinositol transfer activity and a IO-fold higher affinity for this phospholipid. The possibility that the 38 kDa protein is a precursor of the phosphatidylinositol transfer protein is discussed.(C) 1990 Elsevier Science B.V. All rights reserved ...

Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability.

PLSCR3 (phospholipid scramblase 3 Scr3) is one of the superfamily of membrane-associated transcription regulators named Tubby-like protein (TULPs). medium LAQ824 by means of extracellular microvesicles (exosomes). Alternatively Scr3 expression didnt decrease as well as the secretion of Scr3 significantly?in 3T3 Swiss-albino fibroblasts (a parental cell-line of 3T3-L1) had not been increased by differentiation treatment. Overexpression of human being Scr3 during 3T3-L1 differentiation suppressed triacylglycerol build up and inhibited induction from the mRNAs lately stage pro-adipogenic transcription elements [CCAAT/enhancer-binding proteins α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ)] and X-box-binding proteins 1 (XBP1). Manifestation of early stage pro-adipogenic transcription elements (C/EBPβ and C/EBPδ) had not been considerably affected. These outcomes claim that Scr3 features as a poor regulator of adipogenesis in 3T3-L1 cells at a particular differentiation ...

The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.[1] ...

We have reported studies characterizing small-molecule inhibitors that selectively inhibit PLTP activity and concomitantly reduce apoB secretion. In the present study, we identified small molecules that inhibit both PLTP and MTP activities, which are known to regulate apoB secretion. This is the first report to identify dual inhibitors for PLTP and MTP activities. The discovery was not expected based on the lack of homology of PLTP and MTP at protein sequence levels. Although CETP and PLTP have 40% homology and belong to the family of lipid transfer/lipopolysaccharide-binding proteins (Tollefson et al., 1988; Day et al., 1994), none of these compounds inhibit CETP activity (Luo et al., 2010). MTP and apoB belong to the vitellogenin family of lipid transfer proteins. Read et al. (2000) predicted the three-dimensional structure of the C-terminal lipid binding cavity of MTP based on the crystal structure of lipoviellin. The lipid cavity in MTP bears a resemblance to the lipid binding domain of ...

PITPNB antibody, Internal (phosphatidylinositol transfer protein beta) for WB. Anti-PITPNB pAb (GTX45365) is tested in Human samples. 100% Ab-Assurance.

Progression of proteins through the secretory pathway of eukaryotic cells involves a continuous rearrangement of macromolecular structures made up of proteins and phospholipids. The protein SEC14p is essential for transport of proteins from the yeast Golgi complex. Independent characterization of th …

Lipopolysaccharides (LPS) of the cell wall of Gram (-) bacteria trigger inflammation, which is associated with marked changes in glucose metabolism. Hyperglycemia is frequently observed during bacterial infection and it is a marker of a poor clinical outcome in critically ill patients. The aim of the present study was to investigate the effect of an acute injection or continuous infusion of LPS on experimentally-induced hyperglycemia in wild-type and genetically-engineered mice. The acute injection of a single dose of LPS produced an increase in glucose disposal and glucose-stimulated insulin secretion (GSIS). Continuous infusion of LPS through mini-osmotic pumps was also associated with increased GSIS. Finally, manipulation of LPS detoxification by knocking out the plasma phospholipid transfer protein (PLTP) led to increased glucose disposal and GSIS. Overall, glucose tolerance and GSIS tests supported the hypothesis that mice treated with LPS develop glucose-induced hyperinsulinemia. The ...

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Janis MT; Siggins S; Tahvanainen E; Vikstedt R; Silander K; Metso J; Aromaa A; Taskinen MR; Olkkonen VM; Jauhiainen M; Ehnholm C (2004 ...

Sigma-Aldrich offers abstracts and full-text articles by [Hiroyuki Takatsu, Keiko Baba, Takahiro Shima, Hiroyuki Umino, Utako Kato, Masato Umeda, Kazuhisa Nakayama, Hye-Won Shin].

Our laboratory is focusing on a family of proteins called phospholipid scramblases. We originally cloned phospholipid scramblase 1 (PLSCR1), a Ca2+-binding, endofacial plasma membrane protein, based on its capacity to promote rapid transbilayer movement of phospholipids in response to elevated Ca2+. Such redistribution of phospholipids is normally observed upon platelet activation, and in injured or apoptotic cells. We subsequently identified three additional members of this gene family. Recent data from our laboratory suggest a considerably more complex biology for these proteins than their putative role in mediating transbilayer lipid movement. PLSCR1 -- and possibly other members of the PLSCR gene family -- plays a role in modulating the signal transduction through multiple growth factor receptors, and is itself transcriptionally upregulated through these same growth factor receptor pathways. We also discovered that when transcriptionally induced, a portion of the newly synthesized PLSCR1 ...

Phospholipid transfer protein (PLTP), which binds phospholipids and facilitates their transfer between lipoproteins in plasma, plays a key role in lipoprotein remodeling. PLTP levels increase during acute inflammation and increased PLTP activity is associated with the inflammatory markers C-reactive protein (CRP) and serum amyloid A (SAA) as seen in patients with type 2 diabetes and cardiovascular disease. SAA, an HDL apolipoprotein, is highly induced during inflammation. In this study we investigated whether HDL remodeling by PLTP is affected by SAA, and the significance of PLTP on HDL biogenesis. Over-expression of PLTP in mice using an adenoviral vector reduced HDL-C and phospholipid (PL) in a dose dependent manner with the liberation of lipid-poor apoA-I. Co-expression of PLTP and SAA produced a significantly greater reduction in HDL-C and PL than expression of either PLTP or SAA alone. Studies were carried out to examine the lipidation of apoA-I and formation of nascent HDL particles by ...

Shishova, E.Y., Stoll, J.M., Ersoy, B.A., Shrestha, S., Scapa, E.F., Li, Y., Niepel, M.W., Su, Y., Jelicks, L.A., Stahl, G.L., Glicksman, M., Gutierrez-Juarez, R., Cuny, G.D., Cohen, D.E. Stahl, G.L., Cuny, G.D., Cohen, D.E. Genetic Ablation or Chemical Inhibition of Phosphatidylcholine Transfer Protein Attenuates Diet-Induced Hepatic Glucose Production. Hepatology 2011, 54, 664 - 674.. Shishova, E.Y., Di Costanzo, L., Emig, F. A., Ash, D. E., Christianson, D.W. Probing the Specificity Determinants of Amino Acid Recognition by Arginase. Biochemistry 2009, 38, 121-131.. Wagle N., Xian, J., Shishova, E.Y., Wei, J., Glicksman, M.A., Cuny, G.D., Stein. R.L., Cohen. D.E. Small-molecule inhibitors of phosphatidylcholine transfer protein/StarD2 identified by high-throughput screening. Anal. Biochem. 2008, 383, 85-92.. Shishova E.Y., Yu F., Miller D.J., Faraldos J.A., Zhao Y., Coates R.M., Allemann R.K., Cane D.E., Christianson D.W. X-ray Crystallographic Studies of Substrate Binding to Aristolochene ...

These reference sequences exist independently of genome builds. Explain. These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above. ...

Nuclear receptors play prominent roles in regulating lipid homeostasis. Fibrates as a drug class effectively reduce plasma triglyceride levels and moderately elevate HDL cholesterol. These effects have been largely attributed to their activation of PPARα (Staels et al., 1998). LXRs on the other hand, are novel receptors that have been regarded as the master transcription factors mediating cholesterol catabolism (Repa and Mangelsdorf, 2002). In this report, we have investigated the effects of nuclear receptor interplay on lipid homeostasis in vivo. We have shown that simultaneous activation of PPARα and LXRs led to significant accumulation of enlarged HDL cholesterol that is enriched in apoE and apoAI. The enlarged HDL cholesterol is closely associated with liver PLTP mRNA induction and the elevation of plasma PLTP activity. Coadministration of PPARα and LXR agonists also reduced plasma triglycerides and produced virtually no changes in liver triglyceride levels through intricate modulation of ...

Results 202 proteins showed significant differential expression between the CAD patients and the control subjects (P , 0.05). CAD patients had selective depletion of antioxidants; glutathione peroxidase 3 (GPX3) (p , 1e-28), clusterin (p , 1e-12) and serum paroxonase-1 (PON1) (p , 1e-7) compared with controls. Furthermore, there was selective up-regulation of proteins concerned with inflammation; serum amyloid A-1 (p , 1e-12), mannan binding lectin serine protease 1 (MASP1) (p , 1e-8) and galectin-3-binding protein (p = 0.001) in the CAD patients compared with the control subjects. Phospholipid transfer protein (PLTP) (p , 0.001) and apolipoprotein (a) (p = 0.002) were over expressed in the CAD patients. ...

PLTP antibody, C-term (phospholipid transfer protein) for FACS, IHC-P, WB. Anti-PLTP pAb (GTX81352) is tested in Human samples. 100% Ab-Assurance.

Autophagy, Epidermal Growth Factor, Starvation, Dimerization, Endocytosis, Phospholipid Transfer Protein, Plasma, Lipoproteins, Metabolism, Mice, Disease, Lipoprotein, Risk, and Associations

Complete information for PITPNM2 gene (Protein Coding), Phosphatidylinositol Transfer Protein Membrane Associated 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Polyene phosphatidylcholine is an unsaturated version of phosphatidylcholine which is derived from natural sources such as soybeans, eggs, sunflowers, mustard, etc. It is interchangeably mentioned as lecithin which is a different chemical moiety. It has a vital role in the transfer of phosphatidylcholine transfer protein (PCTP) within cell membranes and is the activation of enzymes having significance in the metabolic functions of the liver.. Liver diseases are reigning the clinical application segment for the polyene phosphatidylcholine market. According to the latest statistics provided by the National Institute of Health (NIH), it is estimated that approximately 50 million people worldwide are suffering from chronic liver disease. Additionally, the growing incidence of hepatitis B infection among the adult population across the globe further accelerates the market growth. Dietary supplements are gaining immense popularity since the last 2 decades on account of the rising public health ...

lipid transfer protein: accelerates glycolipid exchange; catalyzes net transfer of glycosphingolipids from brush border membrane vesicles; also facilitates transfer of glucosyl-, galactosyl- & lactosylceramide from liposomal vesicles to red ghost cells; see also record for phospholipid exchange protein

Flippases are proteins in cell membranes that mediate the transfer of specific phospholipids or fats from one sheet to another sheet in the cell membrane. This displacement of lipid molecules in cell membranes requires an energy supply and induces an electric current. And this microcurrent could play a key role in controlling certain essential processes in the body and its health, suggests, this study from the University of Aarhus (Denmark).. This electric current, hitherto unknown, develops in the cells of the body precisely when the flippases mediate the transfer of lipids from the external layer to the internal layer of the cell membranes of the body. This electric current seems to be important for a whole range of other cellular mechanisms, as well as for human well-being and health. In any case, this is the conclusion of Professor Jens Peter Andersen of the Department of Biomedicine at the University of Aarhus and his team.. Why you shouldnt underestimate ...

Lipid Transfer Proteins (LTPs) are found in plants and foods that contain plants. Lipid Transfer Protein Syndrome is an allergy affecting people who have become sensitised to LTPs.

Plasmid PITPbeta-GFP from Dr. Shamshad Cockcrofts lab contains the insert PITP beta and is published in Biochem J. 2006 Sep 15;398(3):411-21. This plasmid is available through Addgene.

Phosphatidylcholine transfer protein (PC-TP) is a phospholipid-binding protein that is enriched in liver and that interacts with thioesterase superfamily member 2 (THEM2). Mice lacking either protein exhibit improved hepatic glucose homeostasis and are resistant to diet-induced diabetes. Insulin receptor substrate 2 (IRS2) and mammalian target of rapamycin complex 1 (mTORC1) are key effectors of insulin signaling, which is attenuated in diabetes. We found that PC-TP inhibited IRS2, as evidenced by insulin-independent IRS2 activation after knockdown, genetic ablation, or chemical inhibition of PC-TP. In addition, IRS2 was activated after knockdown of THEM2, providing support for a role for the interaction of PC-TP with THEM2 in suppressing insulin signaling. Additionally, we showed that PC-TP bound to tuberous sclerosis complex 2 (TSC2) and stabilized the components of the TSC1-TSC2 complex, which functions to inhibit mTORC1. Preventing phosphatidylcholine from binding to PC-TP disrupted ...

PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.

6. CONCLUSIONS 1. We found that mast cell stimulation can induce PS externalization in the absence of secretory response. 2. We identified GPI-APs as molecules whose engagement can induce sustained and reversible non-apoptotic PS externalization. 3. GPI-AP-induced PS externalization was determined as non- apoptotic and distinct from the FceRl-induced PS externalization. 4. The effect of multiple triggering on PS externalization was additive and dependent on a tlpe of stimulus and cells engaged. 5. We identifred PLSCR1 as a molecule that becomes tyrosine phosphorylated in mast cells stimulated through GPI-APs. 6. We found that the PLSCR1 tyrosine phosphorylation is not associated with mast cell secretory response' and with GPI-AP- or FceRl-induced non-apoptotic PS externalization. 7. Using confocal microscopy and electron microscopy visualization of PLSCR1 in the course of mast cell activation we found that PLSCR1: (1) is not co-localized with extemalized PS, (2) is not co- localized with ...

In order to study the individual steps involved in the import of phosphatidylcholine (PC) into rat liver mitochondria, a number of PC analogues were introduced into the outer membrane of isolated mitochondria. Two fluorescent PC species, i.e. 1-palmitoyl-2-(16-bimanylthio)hexadecanoyl-PC (bimane-PC) and 1-palmitoyl-2-(10-pyrene)decanoyl-PC (pyrene-PC), and one radiolabeled PC species, i.e. 1-palmitoyl-2-[1-14C]oleoyl-PC (14C-POPC), were studied. The PC analogues were introduced from small unilamellar vesicles with the use of PC-specific transfer protein. The amount of PC imported was quantified by reisolation of the mitochondria. Import of the fluorescent PC species was monitored by on-line fluorescence spectroscopy. The distribution of the newly inserted PC between the outer and the inner membrane was assessed by separation of the two membranes using digitonin treatment. All analogues tested remained exclusively localized in the outer membrane thereby suggesting that additional ...

Background Haptoglobin is a plasma proteins that scavenges haemoglobin during haemolysis. not really in normolipidemic human being plasma. Haptoglobin amounts and PLTP activity are inversely proportional in hyperlipidemic plasmas (R2 = 0.57, p 0.05). Once the PLTP activity was graphed versus the Horsepower/Apo-A1 percentage in hyperlipidemic plasma there is a significant relationship (R2 = 0.69, p 0.05) recommending that PLTP activity is suffering from the combined aftereffect of Apo-A1 and haptoglobin. When haptoglobin was put into specific hyperlipidemic plasma samples there was a dose dependent decrease in PLTP activity. In these samples we also found a negative Zibotentan correlation (-0.59, p 0.05) between PLTP activity and Hp/Apo-A1. Rabbit Polyclonal to ENTPD1 When we added an amount of haptoglobin equivalent to 100% of the basal levels, we found a 64 23% decrease (p 0.05) in PLTP activity compared to basal PLTP activity. We tested the hypothesis that additional Apo-A1 would induce PLTP ...

Description: The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012 ...

We examined the discussion of ECM1 (extracellular matrix protein 1) using yeast two-hybrid screening and identified the type II transmembrane protein, PLSCR1 (phospholipid scramblase 1), as a binding partner. we were able to demonstrate PLSCR1-ECM1 interaction in human skin extracts. Furthermore, whereas ECM1 is secreted by the endoplasmic/Golgi-dependent pathway, PLSCR1 release from HaCaT keratinocytes occurs via a lipid raft-dependent mechanism, and is deposited in the extracellular matrix. In summary, we here demonstrate that PLSCR1 interacts with the tandem repeat region of ECM1a in the dermal epidermal junction zone of human skin and provide for the first time experimental evidence that PLSCR1 is secreted by an unconventional secretion pathway. These data suggest that PLSCR1 is a multifunctional protein that can function both inside and outside of the cell and together with MK-4827 ECM1 may play a regulatory role in human skin. MK-4827 gene (11 exons) is located on chromosome 1q21.2 (1, 2) ...

LIPID TRANSFER PROTEINS AND MEMBRANE CONTACT SITES IN HUMAN CANCER FEBRUARY 12, 2020: Presentation from Sima Lev about the lipid transfer proteins in cancer.

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to another. This gene is maternally expressed. It maps within the most common interval of deletion responsible for Angelman syndrome, also known as happy puppet syndrome. [provided by RefSeq, Jul 2008 ...

Human phospholipid screen IgG/IgM ELISA kit detects IgG and IgM autoantibodies to screen beta-2 glycoprotein I and anti phospholipid IgG/IgM.

Eukaryotes possess seven different phosphoinositides (PIPs) that help form the unique signatures of various intracellular membranes. PIPs serve as docking sites for the recruitment of specific proteins to mediate membrane alterations and integrate various signaling cascades. The spatio-temporal regulation of PI kinases and phosphatases generates distinct intracellular hubs of PIP signaling. Hepatitis C virus (HCV), like other plus-strand RNA viruses, promotes the rearrangement of intracellular membranes to assemble viral replication complexes. HCV stimulates enrichment of phosphatidylinositol 4-phosphate (PI4P) pools near endoplasmic reticulum (ER) sites by activating PI4KIIIα, the kinase responsible for generation of ER-specific PI4P pools. Inhibition of PI4KIIIα abrogates HCV replication. PI4P, the most abundant phosphoinositide, predominantly localizes to the Golgi and plays central roles in Golgi secretory functions by recruiting effector proteins involved in transport vesicle generation. The PI4P

Microbubble-assisted ultrasound (sonopermeabilization) results in reversible permeabilization of the plasma membrane of cells. This method is increasingly used in vivo because of its potential to deliver therapeutic molecules with limited cell damage. Nevertheless, the effects of sonopermeabilization on the plasma membrane remain not fully understood. We investigated the influence of sonopermeabilization on the transverse mobility of phospholipids, especially on phosphatidylserine (PS) externalization. We performed studies using optical imaging with Annexin V and FM1-43 probes to monitor PS externalization of rat glioma C6 cells. Sonopermeabilization induced transient membrane permeabilization, which is positively correlated with reversible PS externalization. This membrane disorganization was temporary and not associated with loss of cell viability. Sonopermeabilization did not induce PS externalization via activation of the scramblase. We hypothesize that acoustically induced membrane pores may

Protein features are: Cellular retinaldehyde binding/alpha-tocopherol transport; Cellular retinaldehyde-binding/triple function, C-terminal; Phosphatidylinositol transfer protein-like, N-terminal ...

Each molecule that makes up either bilayer is shaped vaguely like a gooey flexible fat headed thumbtack. The head of the thumbtack is called a leaflet, and these leaflets make up the surface of the membrane itself. The tack part of each leaflet molecule points inward toward the middle of the space between the membranes. It is actually divided into two thin little tails, so my tack analogy was pretty bad. Now let me introduce you to flippase, an enzyme that lives among these tails in the space between the layers. He does not fit what you think you know about enzymes. He does not hold two or more molecules together in a way that will allow chemical reactions to take place rapidly between them. Flippase is clearly a machine with moving parts that do things, but what we think of as the usual enzyme thing is not the flippase thing. When the cell has built all or part of the first layer of the lipid bilayer membrane, the only layer it can reach, flippase grabs molecules and flips them to the layer ...

BACKGROUND:Excessive alcohol consumption can cause hepatocellular injury. ATPase II (ATP8A1) can display an ATP-dependent phospholipid translocase activity. However, the function of ATP8A1 in hepatocyte injury is still unclear. In the present study w...

(KudoZ) English to Dutch translation of tick the box (not literally!): onzin ophangen [memo from manager to his employees (Bus/Financial)].

Ive heard flip-flops can cause injuries, but kids wear them all summer. How can parents make them as safe as possible? Flip-flops are functional for use around the pool but ideally shouldnt be worn