Learn about Phosphoglycerate Kinase Deficiency and other health conditions at HealtheTreatment. What is Phosphoglycerate Kinase Deficiency? Compare Phosphoglycerate Kinase Deficiency symptoms Phosphoglycerate Kinase Deficiency treatments and . Share online in the health community.
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
TY - JOUR. T1 - Anion activation of 3-phosphoglycerate kinase requires domain closure. AU - Szilágyi, Andrea N.. AU - Vas, M.. PY - 1998/6/9. Y1 - 1998/6/9. N2 - 3-Phosphoglycerate kinase is a typical two-domain hinge-bending enzyme, which is known to be regulated by multivalent anions. Here a relationship between this regulation and the hinge-bending domain closure is proposed on the basis of enzyme kinetic analysis and molecular modeling. Activation of the pig muscle enzyme at low concentrations and inhibition at high concentrations of various anionic analogues of the substrate 3- phosphoglycerate or of the nonsubstrate metal-free ATP are described by a two-site model assuming separate sites for activation and inhibition, respectively. Kinetic experiments with various pairs of analogues suggest the presence of a common site for activation by all effectors, separate from the catalytic site for 3-phosphoglycerate; and a common site for inhibition, except for metal-free ATP, identical with the ...
The Phosphoglycerate Kinase 1 is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19.PGK1 is distinguished from testicular PGK2, which maps to chromosome 6p21. The deduced protein contains 417 amino acid residues. Southern blot analysis of human genomic DNAs showed a complex pattern of hybridizing fragments, 2 of which were non-X in origin. The results were interpreted as reflecting the existence of a small family of dispersed PGK or PGK-like genes.
Phosphoglycerate kinase (PGK) mammalian promoter for constitutive expression. The PGK promoter is upstream of our normal multiple cloning site.
Peritoneal carcinomatosis is a frequent finding in gastric cancer associated with a poor prognosis. The features that enable gastric tumors to disseminate are poorly understood until now. Previously, we showed elevated mRNA levels of phosphoglycerate kinase 1 (PGK1), an ATP-generating enzyme in the glycolytic pathway, the chemokine receptor 4 (CXCR4), the corresponding chemokine ligand 12 (CXCL12) and beta-catenin in specimens from gastric cancer patients with peritoneal carcinomatosis. In this study the influence of PGK1 on CXCR4 and beta-catenin was assessed as well as the invasiveness of PGK1 overexpressing cancer cells. In this current study we found that PGK1 regulates the expression of CXCR4 and beta-catenin at the mRNA and protein levels. On the other hand, CXCR4 regulates the expression of PGK1. Plasmid-mediated overexpression of PGK1 dramatically increased the invasiveness of gastric cancer cells. Interestingly, inhibition of CXCR4 in cells overexpressing PGK1 produced only a moderate ...
The mouse autosomal locus that determines the form of phosphoglycerate kinase found only in testes is shown here to be closely linked to but not included within the major histocompatibility complex on chromosome 17. Data are presented that strongly favor the location of this locus, designated Pgk-2, distal to H-2, Qa-1, and Qa-2, and closely associated with T1a. The Pgk-2 strain distribution pattern for 103 inbred and congenic strains of mice is given. Because Pgk-2 is polymorphic among inbred strains, it should be of value in linkage studies.
Recombinant Phosphoglycerate Kinase 2 (PGK2) Protein (His tag). Species: Human. Source: Escherichia coli (E. coli). Order product ABIN1981682.
testis specific phosphoglycerate kinase: human testis-specific isoenzyme; amino acid sequence in first source; RefSeq NM_138733 (human), NM_031190 (mouse); GenBank AY496962 and AY500132 (S scrofa)
Top performende anti-Yeast (Saccharomyces cerevisiae) Phosphoglycerate Kinase 1 Antikörper für Immunofluorescence (IF) vergleichen & kaufen.
The SCOP classification for the Phosphoglycerate kinase superfamily including the families contained in it. Additional information provided includes InterPro annotation (if available), Functional annotation, and SUPERFAMILY links to genome assignments, alignments, domain combinations, taxonomic visualisation and hidden Markov model information.
en] The cold-active phosphoglycerate kinase from the Antarctic bacterium Pseudomonas sp. TACII18 exhibits two distinct stability domains in the free, open conformation. It is shown that these stability domains do not match the structural N- and C-domains as the heat-stable domain corresponds to about 80 residues of the C-domain, including the nucleotide binding site, whereas the remaining of the protein contributes to the main heat-labile domain. This was demonstrated by spectroscopic and microcalorimetric analyses of the native enzyme, of its mutants, and of the isolated recombinant structural domains. It is proposed that the heat-stable domain provides a compact structure improving the binding affinity of the nucleotide, therefore increasing the catalytic efficiency at low temperatures. Upon substrate binding, the enzyme adopts a uniformly more stable closed conformation. Substrate-induced stability changes suggest that the free energy of ligand binding is converted into an increased ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Pgk-1 is an X-linked gene encoding 3-phosphoglycerate kinase, an enzyme necessary in every cell for glycolysis. The regulatory sequences of the Pgk-1 gene were used to drive the E. coli lacZ reporter gene and 2 strains of transgenic animals created with this Pgk-lacZ transgene carried on autosomes. …
Pgk1 - Pgk1 (untagged ORF) - Rat phosphoglycerate kinase 1 (Pgk1), (10 ug) available for purchase from OriGene - Your Gene Company.
mouse connexin45(Cx45) genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), mouse phosphoglycerate kinase promoter (PGK promoter), Phage P1 loxP, E. coli neo, E. coli ...
Phosphoglycerate kinase (EC 2.7.2.3)(PGK) catalyzes the second step in the second phase of glycolysis, the reversible conversion of 1,3-diphospho-glycerate to 3-phosphoglycerate with generation of one molecule of ATP. PGK is found in all living organisms and its sequence has been highly conserved throughout evolution. It is a two-domain protein; each domain is composed of six repeats of an alpha/beta structural motif ...
Hinge-bending movements in proteins comprising two or more domains form a large class of functional movements. Hinge-bending regions demarcate protein domains and collectively control the domain movement. Consequently, the ability to recognise sequence features of hinge-bending regions and to be able to predict them from sequence alone would benefit various areas of protein research. For example, an understanding of how the sequence features of these regions relate to dynamic properties in multi-domain proteins would aid in the rational design of linkers in therapeutic fusion proteins. The DynDom database of protein domain movements comprises sequences annotated to indicate whether the amino acid residue is located within a hinge-bending region or within an intradomain region. Using statistical methods and Kernel Logistic Regression (KLR) models, this data was used to determine sequence features that favour or disfavour hinge-bending regions. This is a difficult classification problem as the number of
Generation of α1−/− mice. The GABAA receptor α1 gene-targeting vector was constructed from the same genomic 129/SvEv λ fixII clone, which has been used for the introduction of the α1H101R mutation (McKernan et al., 2000). However, for the complete gene knock-out, exon 4 was disrupted at the MscI restriction site by cloning the 1.2 kb BstBI+MscI and the 7 kbEcoRV+BamHI DNA fragment blunt-ended into the targeting vector. A phosphoglycerate kinase I (PGK) neo and a thymidine kinase (TK) cassette were also engineered blunt-ended into the loxP site containing targeting vector. After linearization with NotI the targeting vector was introduced into AB2.2 embryonic stem (ES) cells (Lexicon Genetics) as described (Soriano et al., 1991; Rosahl et al., 1993, 1995). Homologous recombinants were identified by PCR using the primers 5′-ATTAATGGAGAGTGTGGTAATCTTT-3′ and 5′-GGATGCGGTGGGCTCTATGGCTTCTGA-3′ and were further confirmed by genomic Southern blotting. Correctly targeted ES cell clones ...
PGK1 an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein). Note: This description may include information from UniProtKB ...
1. A study has been made of the ability of rat liver in vivo to maintain equilibrium in the combined glyceraldehyde 3-phosphate dehydrogenase, 3-phosphoglycerate kinase and lactate dehydrogenase reactions, i.e. in the system: [Formula: see text] Attempts were made to upset equilibrium. The [lactate]/[pyruvate] ratio was rapidly changed by injection of ethanol or crotyl alcohol, and the value of [ATP]/[ADP][HPO42-] was rapidly changed by injection of ethionine or carbonyl cyanide p-trifluoromethoxy-phenylhydrazone. 2. The concentrations of the metabolites occurring in the above equation were measured in freeze-clamped liver. 3. Although the injected agents caused large changes in the concentrations of the individual components, near-equilibrium in the system was maintained, as indicated by the fact that the value of [ATP]/[ADP][HPO42-], referred to as the phosphorylation state of the adenine nucleotides, measured directly agreed with the value calculated for equilibrium conditions from the above ...
Phosphoglycerate Phosphokinase antibody LS-C147605 is a biotin-conjugated rabbit polyclonal antibody to yeast Phosphoglycerate Phosphokinase. Validated for ELISA, IF, IHC and WB.
This protein is a key enzyme of primary carbon metabolism. The catalytic activity of pgm is the following: 3-Phosphoglycerate to 2-Phosphoglycerate (reversible). By similarity it has been ascertained to have an absolute requirement for manganese (Mn). In B.subtilis this protein is found in an operon containing (in order: pgk, tpi, pgm and eno). See the following gene records for the remaining glycolytic enzymes: UU181 (tpi), UU184 (eno) and UU279 (pgk ...
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Expression-ready Rat PGK1 cDNA ORF clone (RG80663-CF) with enhanced promotor in expression vector (pCMV3-C-FLAG) is confirmed by full-length sequence and validated in expression capability for gene expression studies or other applications. Quote for bulk production.
Hello, I am looking for a source of the plasmid pGK12 as prepared by Kok et al, ref. Appl Envir Microbiol, V 48 N 4, 1984. If any one has any suggestions or sources for this plasmid, please contact by e-mail to dtjones at comp.uark.edu. Thank you. dtj ...
Plasmid pLenti.PGK.blast-Flag-MFF-WT from Dr. Reuben Shaws lab contains the insert Flag-MFF and is published in Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138. This plasmid is available through Addgene.
Primer recognition proteins (PRP) are cofactors for DNA polymerase alpha and may have a role in lagging-strand DNA replication. PRP is composed of two subunits, which we have previously identified as the protein-tyrosine kinase substrate annexin II and phosphoglycerate kinase (PGK). In this study, we have examined the physiological involvement of these proteins in DNA synthesis and cell proliferation. When exponentially growing human HeLa cells are exposed to antisense phosphorothioate oligodeoxynucleotides to annexin II, ongoing DNA synthesis is reduced. The extent of reduction with antisense oligodeoxynucleotide to PGK was much less than with the antisense annexin II oligodeoxynucleotide. Reductions in the labeling and mitotic indices of HeLa cell cultures are seen after exposure to antisense oligodeoxynucleotides. Flow cytometric analyses indicate that progression from S phase to G2 phase of the cycle is retarded by exposure of cells to the antisense oligodeoxynucleotides. Corresponding sense ...
cytosol, ADP binding, ATP binding, phosphoglycerate kinase activity, gluconeogenesis, glycolytic process, positive regulation of oxidative phosphorylation
Clonal expansions of mutated hematopoietic cells, termed clonal hematopoiesis, are common in aging humans. One expected consequence of mutation-associated clonal hematopoiesis is an increased risk of hematologic cancers, which has now been shown in several studies. However, the hematopoietic stem cells that acquire these somatic mutations also give rise to mutated immune effector cells, such as monocytes, granulocytes, and lymphocytes. These effector cells can potentially influence many disease states, especially those with a chronic inflammatory component. Indeed, several studies have now shown that clonal hematopoiesis associates with increased risk of atherosclerotic cardiovascular disease. Emerging data also associate clonal hematopoiesis to other non-hematologic diseases. Here, we will review recent studies linking clonal hematopoiesis to altered immune function, inflammation, and non-malignant diseases of aging ...
Hi there, The article posted by Krystyna Kielan Rybicka on the bionet.parasitology newsgroup on the existence of glycogen containing particles called glycosomes in almost all animal is interesting but provokes a large amount of confusion. As you probably all know glycosomes are the membrane surrounded microbody-like organelles of trypanosomatid and bodonid flagellated protists that belong to the order of the Kinetoplastida. This organelle, is unique to the Kinetoplastida. Glycosomes contain the early enzymes of the glycolytic pathway and glycerol metabolism, such as hexokinase, phosphoglucose isomerase, phosphofructokinase, aldolase, triosephosphate isomerase, glyceraldehyde-phosphate dehydrogenase, phosphoglycerate kinase, glycerol kinase and glycerophosphate dehydrogenase. They also contain enzymes involved in such diversed pathways as carbon-dioxide fixation, pyrimidine biosynthesis, ether-lipid biosynthesis and purine salvage. The organelles resemble the peroxisomes of other eukaryotic ...
Our study extends the understanding of the genetic predispositions underlying BCR-ABL1-negative MPNs and JAK2 V617F clonal hematopoiesis in the general population. In addition to the 46/1 JAK2 haplotype, we identify predisposition alleles associated with TERT, SH2B3, TET2, ATM, CHEK2, PINT, and GFI1B in both MPN patients and in population controls who are V617F carriers. These genes impact diverse biologic pathways such as cellular aging (TERT), JAK-STAT signaling (JAK2, SH2B3), epigenetic regulation (TET2), DNA damage repair and/or tumor suppressor function (ATM, CHEK2, PINT), and erythroid/ megakaryocyte development (GFI1B).. Age-related clonal hematopoiesis in the general population was recently found to be associated with adverse outcomes, including an increased risk of hematologic cancer and all-cause mortality.27⇓-29,37 JAK2 was among the most commonly mutated genes in these studies, which also included DNMT3A, TET2, ASXL1, and TP53. The predisposition alleles we have identified in our ...
The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015 ...
Shop Phosphoglycerate transport system transcriptional regulatory protein ELISA Kit, Recombinant Protein and Phosphoglycerate transport system transcriptional regulatory protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
A new view has recently emerged showing that multi-domain enzymes are biological machines evolved to harness stochastic kicks of solvent particles into highly directional functional motions. These intrinsic motions are structurally encoded, and Nature makes use of them to catalyze chemical reactions by means of ligand-induced conformational changes and states redistribution. Such mechanisms align reactive groups for efficient chemistry and stabilize conformers most proficient for catalysis. By combining single-molecule Foerster resonance energy transfer (smFRET) measurements with normal mode analysis and coarse grained mesoscopic simulations, we obtained results for a hinge-bending enzyme, namely phosphoglycerate kinase (PGK), which support and extend these new ideas. From smFRET, we obtained insight into the distribution of conformational states and the dynamical properties of the domains. With the simulations, we characterize the inter-domain motions of a compact state of PGK. The data show ...
CHIP stands for Clonal Hematopoiesis of Indeterminate Significance (1-4). Up to 20% of individuals in the general population acquire mutations in their bone marrow stem cells as they age that give that population of cells a survival or clonal advantage for growth. The frequency of CHIP may be higher in patients with other cancers. CHIP increases with age, and has been shown to be a risk factor associated with cardiovascular disease and a tendency to the development of bone marrow cancers at a rate of 1% per year (1,2,5). CHIP is also associated with the development of bone marrow cancers that occur after chemotherapy. The investigators want to investigate whether CHIP is also a risk factor for chemotherapy-related complications like low blood counts, infections, cardiac events, hospitalizations, dose delays and dose reductions. They are also interested in determining if CHIP may explain why some patients do not recover normal blood counts after chemotherapy finishes.. The results from this ...
This study explores the prevalence of clonal hematopoiesis related to radioactive iodine exposure and how it impacts overall survival in patients with thyroid cancer.
Sperm proteins are important for the sperm cell function in fertilization. Some of them are involved in the binding of sperm to the egg. We characterized the acrosomal sperm protein detected by a monoclonal antibody (MoAb) (Hs-8) that was prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperms and we tested the possible role of this protein in the binding assay. Indirect immunofluorescence and immunogold labelling, gel electrophoresis, Western blotting and protein sequencing were used for Hs-8 antigen characterization. Functional analysis of GAPDHS from the sperm acrosome was performed in the boar model using sperm/zona pellucida binding assay. Monoclonal antibody Hs-8 is an anti-human sperm antibody that cross-reacts with the Hs-8-related protein in spermatozoa of other mammalian species (boar, mouse). In the immunofluorescence test, Hs-8 antibody recognized the protein localized in the acrosomal part of the sperm head and in the principal piece of the sperm flagellum. In
The behaviour of human cells arrested in mitosis can be severely perturbed so as to generate numerous small minisegregants containing very few chromosomes. These cells can be separated according to size and DNA content and fused with intact cells. In this paper we describe the production and some properties of proliferating cell hybrids generated by fusion of human minisegregant cells derived from a HeLa strain with mouse A9 cells deficient in hypoxanthine phosphoribosyltransferase (HPRT, EC 2.4.2.8). The hybrids were shown to contain up to 10 human chromosomes including a single X. Independently derived hybrid clones were quantitatively characterized and compared with the parental phenotypes with respect to HPRT. Human isozymes of each of the 3 enzymes HPRT, glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and phosphoglycerate kinase (EC 2,7.2.3) were found. Tests to evaluate both structure and function of HPRT were utilized. The specific activity of HPRT of more than 10 hybrids tested was ...
This is a consultative evaluation in which the case will be evaluated at Mayo Medical Laboratories, the appropriate tests performed at an additional charge, and the results interpreted.. Note: RBCE / Reflexed RBC Enzymes, Blood includes: adenylate kinase, phosphofructokinase, phosphoglycerate kinase, triosephosphate isomerase, and pyrimidine 5nucleotidase.. ...
Increased levels of the phosphoglycerate kinase, an ATP-producing glycolytic enzyme, was detected in feces of food-restricted mice and this enzyme was further increased in the ABA group. Starvation also upregulated several other proteins synthetized by order Clostridiales including Clostridiaceae and Lachnospiraceae families. No significant differences in the in vitro ATP-producing capacity by bacterial proteins from ABA, food-restricted, and ad libitum-fed control mice were found. However, plasma levels of acetate strongly tended to be increased in the activity groups including ABA mice.. ...
To combat decrease in rice productivity under different stresses, an understanding of rice metabolism is needed. Though there are different genome scale metabolic models (GSMs) of Oryza sativa japonica, no GSM with gene-protein-reaction association exist for Oryza sativa indica. Here, we report a GSM, OSI1136 of O.s. indica, which includes 3602 genes and 1136 metabolic reactions and transporters distributed across the cytosol, mitochondrion, peroxisome, and chloroplast compartments. Flux balance analysis of the model showed that for varying RuBisCO activity (Vc/Vo) (i) the activity of the chloroplastic malate valve increases to transport reducing equivalents out of the chloroplast under increased photorespiratory conditions and (ii) glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase can act as source of cytosolic ATP under decreased photorespiration ...
Utilizamos cookies propias y de terceros para mejorar nuestros servicios y mostrarle publicidad relacionada con sus preferencias mediante el análisis de sus hábitos de navegación. Si continua navegando, consideramos que acepta su uso. Puede cambiar la configuración u obtener más información aquí. To improve our services and products, we use "cookies" (own or third parties authorized) to show advertising related to client preferences through the analyses of navigation customer behavior. Continuing navigation will be considered as acceptance of this use. You can change the settings or obtain more information by clicking here. Utilizamos cookies próprios e de terceiros para melhorar nossos serviços e mostrar publicidade relacionada às suas preferências, analisando seus hábitos de navegação. Se continuar a navegar, consideramos que aceita o seu uso. Você pode alterar a configuração ou obter mais informações aqui. ...
K01834 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase [EC:5.4.2.11] , (GenBank) gpmA; 2,3-bisphosphoglycerate-dependent phosphoglycerate ...
Pharmaceutical abatement in a groundwater (GW), surface water (SW), and secondary effluent (SE) by conventional ozonation, the conventional peroxone (O3/H2O2), and the electro-peroxone (E-peroxone) processes was compared in batch tests. SE had significantly more fast-reacting dissolved organic matter (DOM) moieties than GW and SW. Therefore, O3 decomposed much faster in SE than in GW and SW. At specific ozone doses of 0.5-1.5 mg O3/mg dissolved organic carbon (DOC), the application of O3/H2O2 and E-peroxone process (by adding external H2O2 stocks or in-situ generating H2O2 from cathodic O2 reduction during ozonation) similarly enhanced the OH yield from O3 decomposition by ∼5-12% and 5-7% in GW and SW, respectively, compared to conventional ozonation ...
Definition: one of three metabolic pathways for carbon fixation in photosynthesis, along with C4 and CAM. This process converts carbon dioxide and ribulose bisphosphate (RuBP, a 5-carbon sugar) into 3-phosphoglycerate through the following reaction: CO2 + H2O + RuBP → (2) 3-phosphoglycerate This reaction occurs in all plants as the first step of the Calvin-Benson cycle ...
Definition: one of three metabolic pathways for carbon fixation in photosynthesis, along with C4 and CAM. This process converts carbon dioxide and ribulose bisphosphate (RuBP, a 5-carbon sugar) into 3-phosphoglycerate through the following reaction: CO2 + H2O + RuBP → (2) 3-phosphoglycerate This reaction occurs in all plants as the first step of the Calvin-Benson cycle ...
Definition: one of three metabolic pathways for carbon fixation in photosynthesis, along with C4 and CAM. This process converts carbon dioxide and ribulose bisphosphate (RuBP, a 5-carbon sugar) into 3-phosphoglycerate through the following reaction: CO2 + H2O + RuBP → (2) 3-phosphoglycerate This reaction occurs in all plants as the first step of the Calvin-Benson cycle ...
Published on 1/19/2017. Fuster JJ, MacLauchlan S, Zuriaga MA, Polackal MN, Ostriker AC, Chakraborty R, Wu CL, Sano S, Muralidharan S, Rius C, Vuong J, Jacob S, Muralidhar V, Robertson AA, Cooper MA, Andrés V, Hirschi KK, Martin KA, Walsh K. Clonal hematopoiesis associated with TET2 deficiency accelerates atherosclerosis development in mice. Science. 2017 02 24; 355(6327):842-847. PMID: 28104796.. Read at: PubMed ...