High/ Low Phosphate diet I. A five-day low phosphate diet / A five-day low phosphate diet with the addition of a phosphate binder / A five-day high phosphate diet.. II. A five-day low phosphate diet / A five-day high phosphate diet / A five-day low phosphate diet with the addition of a phosphate binder III. A five-day high phosphate diet / A five-day low phosphate diet with the addition of a phosphate binder / A five-day low phosphate diet.. IV. A five-day high phosphate diet / A five-day low phosphate diet / A five-day low phosphate diet with the addition of a phosphate binder.. V. A five-day low phosphate diet with the addition of a phosphate binder / A five-day low phosphate diet / A five-day high phosphate diet.. VI. A five-day low phosphate diet with the addition of a phosphate binder / A five-day high phosphate diet / A five-day low phosphate diet. ...
TY - JOUR. T1 - Differences in the allosteric properties of pure low and high phosphate forms of phosphofructokinase from rat liver.. AU - Sakakibara, R.. AU - Uyeda, K.. PY - 1983/7/25. Y1 - 1983/7/25. N2 - Low phosphate and high phosphate forms of phosphofructokinase (Furuya, E., and Uyeda, K. (1980) J. Biol. Chem. 255, 11656-11659) from rat liver were purified to homogeneity and various properties were compared. The specific activities of these enzymes and their electrophoretic mobilities on polyacrylamide in sodium dodecyl sulfate are the same. A limited tryptic digestion yields products with no change in the enzyme activity but with a reduction in the molecular weight of about 2000. Both low and high phosphate enzymes can be phosphorylated by the catalytic subunit of cAMP-dependent protein kinase, and approximately twice as much [32P]phosphate is incorporated into the low phosphate than the high phosphate enzyme. A comparison of their allosteric kinetic properties reveal that the high ...
BackgroundPlant adaptation to limited phosphate availability comprises a wide range of responses to conserve and remobilize internal phosphate sources and to enhance phosphate acquisition. Vigorous restructuring of root system architecture provides a developmental strategy for topsoil exploration and phosphate scavenging. Changes in external phosphate availability are locally sensed at root tips and adjust root growth by modulating cell expansion and cell division. The functionally interacting Arabidopsis genes, LOW PHOSPHATE RESPONSE 1 and 2 (LPR1/LPR2) and PHOSPHATE DEFICIENCY RESPONSE 2 (PDR2), are key components of root phosphate sensing. We recently demonstrated that the LOW PHOSPHATE RESPONSE 1 - PHOSPHATE DEFICIENCY RESPONSE 2 (LPR1-PDR2) module mediates apoplastic deposition of ferric iron (Fe3+) in the growing root tip during phosphate limitation. Iron deposition coincides with sites of reactive oxygen species generation and triggers cell wall thickening and callose accumulation, which ...
BackgroundPlant adaptation to limited phosphate availability comprises a wide range of responses to conserve and remobilize internal phosphate sources and to enhance phosphate acquisition. Vigorous restructuring of root system architecture provides a developmental strategy for topsoil exploration and phosphate scavenging. Changes in external phosphate availability are locally sensed at root tips and adjust root growth by modulating cell expansion and cell division. The functionally interacting Arabidopsis genes, LOW PHOSPHATE RESPONSE 1 and 2 (LPR1/LPR2) and PHOSPHATE DEFICIENCY RESPONSE 2 (PDR2), are key components of root phosphate sensing. We recently demonstrated that the LOW PHOSPHATE RESPONSE 1 - PHOSPHATE DEFICIENCY RESPONSE 2 (LPR1-PDR2) module mediates apoplastic deposition of ferric iron (Fe3+) in the growing root tip during phosphate limitation. Iron deposition coincides with sites of reactive oxygen species generation and triggers cell wall thickening and callose accumulation, which ...
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Phosphorus is an important macronutrient that is severely lacking in soils. In plants, specific microRNAs (miRNAs) essential for nutrient management and the regulation of stress responses are responsible for the control of many phosphate starvation responses. Further understanding of conserved and species-specific microRNA species has potential implications for the development of crops tolerant to soils with low phosphate. This study identified and characterised phosphate starvation-responsive miRNAs in the native Australian tobacco Nicotiana benthamiana. Small RNA libraries were constructed and sequenced from phosphate-starved plant leaves, stems and roots. Twenty-four conserved miRNA families and 36 species-specific miRNAs were identified. The majority of highly phosphate starvation-responsive miRNAs were highly conserved, comprising of members from the miR399, miR827, and miR2111 families. In addition, two miRNA-star species were identified to be phosphate starvation-responsive. A total of seven
A mutant of Arabidopsis thaliana deficient in the accumulation of inorganic phosphate has been isolated by screening directly for plants with altered quantities of total leaf phosphate. The mutant plants accumulate approximately 5% as much inorganic phosphate, and 24 to 44% as much total phosphate, as wild-type plants in aerial portions of the plant. Growth of the mutant is reduced, relative to wild type, and it exhibits other symptoms normally associated with phosphate deficiency. The phosphate deficiency is caused by a single nuclear recessive mutation at a locus designated pho1. The rate of phosphate uptake into the roots was similar between mutant and wild-type plants over a wide range of external phosphate concentrations. In contrast, when plants were grown in media containing 200 micromolar phosphate or less, phosphate transfer to the shoots of the mutant was reduced to 3 to 10% of the wild-type levels. The defect in phosphate transfer to the shoots could be overcome by providing higher ...
The effects of the Na+ gradient and pH on phosphate uptake were studied in brush-border membrane vesicles isolated from rat kidney cortex. The initial rates of Na(+)-dependent phosphate uptake were measured at pH 6.5, 7.5 and 8.5 in the presence of sodium gluconate. At a constant total phosphate concentration, the transport values at pH 7.5 and 8.5 were similar, but at pH 6.5 the influx was 31% of that at pH 7.5. However, when the concentration of bivalent phosphate was kept constant at all three pH values, the effect of pH was less pronounced; at pH 6.5, phosphate influx was 73% of that measured at pH 7.5. The Na(+)-dependent phosphate uptake was also influenced by a transmembrane pH difference; an outwardly directed H+ gradient stimulated the uptake by 48%, whereas an inwardly directed H+ gradient inhibited the uptake by 15%. Phosphate on the trans (intravesicular) side stimulated the Na(+)-gradient-dependent phosphate transport by 59%, 93% and 49%, and the Na(+)-gradient-independent phosphate ...
Low phosphate in the blood is linked to the risk of heart attack and coronary artery disease, a new study in the journal PLOS One reports. Researchers from the University of Surrey found that insufficient levels of phosphate in the blood may pose a particular danger to cardiovascular health, contradicting previous research in this area, which suggested low volumes of the mineral was beneficial to the heart. The study, using data from the RCGP Research and Surveillance Centre, examined phosphate levels of more than 100,000 patients, over five and nine-year intervals, and the impact on their cardiac health. The researchers
Renal reabsorption of inorganic phosphate (Pi) is mediated by the phosphate transporters NaPi-IIa, NaPi-IIc, and Pit-2 in the proximal tubule brush border membrane (BBM). Dietary Pi intake regulates these transporters; however, the contribution of the specific isoforms to the rapid and slow phase is not fully clarified. Moreover, the regulation of PTH and FGF23, two major phosphaturic hormones, during the adaptive phase has not been correlated. C57/BL6 and NaPi-IIa(-/-) mice received 5 days either 1.2 % (HPD) or 0.1 % (LPD) Pi-containing diets. Thereafter, some mice were acutely switched to LPD or HPD. Plasma Pi concentrations were similar under chronic diets, but lower when mice were acutely switched to LPD. Urinary Pi excretion was similar in C57/BL6 and NaPi-IIa(-/-) mice under HPD. During chronic LPD, NaPi-IIa(-/-) mice lost phosphate in urine compensated by higher intestinal Pi absorption. During the acute HPD-to-LPD switch, NaPi-IIa(-/-) mice exhibited a delayed decrease in urinary Pi ...
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Phosphate starvation compromises electron flow through the cytochrome pathway of the mitochondrial electron transport chain, and plants commonly respond to phosphate deprivation by increasing flow through the alternative oxidase (AOX). To test whether this response is linked to the increase in nitric oxide (NO) production that also increases under phosphate starvation, Arabidopsis thaliana seedlings were grown for 15 d on media containing either 0 or 1mM inorganic phosphate. The effects of the phosphate supply on growth, the production of NO, respiration, the AOX level and the production of superoxide were compared for wild-type (WT) seedlings and the nitrate reductase double mutant nia. Phosphate deprivation increased NO production in WT roots, and the AOX level and the capacity of the alternative pathway to consume electrons in WT seedlings; whereas the same treatment failed to stimulate NO production and AOX expression in the nia mutant, and the plants had an altered growth phenotype. The NO donor S
TY - JOUR. T1 - Development of multifunctional Overhauser-enhanced magnetic resonance imaging for concurrent in vivo mapping of tumor interstitial oxygenation, acidosis and inorganic phosphate concentration. AU - Gorodetskii, Artem A.. AU - Eubank, Timothy D.. AU - Driesschaert, Benoit. AU - Poncelet, Martin. AU - Ellis, Emily. AU - Khramtsov, Valery V.. AU - Bobko, Andrey A.. PY - 2019/8/20. Y1 - 2019/8/20. N2 - Tumor oxygenation (pO2), acidosis (pH) and interstitial inorganic phosphate concentration (Pi) are important parameters of the malignant behavior of cancer. A noninvasive procedure that enables visualization of these parameters may provide unique information about mechanisms of tumor pathophysiology and provide clues to new treatment targets. In this research, we present a multiparametric imaging method allowing for concurrent mapping of pH, spin probe concentration, pO2, and Pi using a single contrast agent and Overhauser-enhanced magnetic resonance imaging technique. The developed ...
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Buy Structure and Functioning of Cluster Roots and Plant Responses to Phosphate Deficiency (9781402004346): NHBS - Edited By: Hans Lambers and Pieter Poot, Kluwer Academic Publishers
TY - JOUR. T1 - Dietary phosphate modulates atherogenesis and insulin resistance in apolipoprotein E knockout mice--brief report. AU - Ellam, Timothy. AU - Wilkie, Martin. AU - Chamberlain, Janet. AU - Crossman, David. AU - Eastell, Richard. AU - Francis, Sheila. AU - Chico, Timothy J A. PY - 2011/9. Y1 - 2011/9. N2 - Epidemiological studies link higher serum phosphate and the phosphatonin fibroblast growth factor 23 with cardiovascular events and atheroma, and they link lower serum phosphate with insulin resistance and the metabolic syndrome. We investigated whether manipulating dietary phosphate influences atherogenesis or insulin sensitivity in mice.. AB - Epidemiological studies link higher serum phosphate and the phosphatonin fibroblast growth factor 23 with cardiovascular events and atheroma, and they link lower serum phosphate with insulin resistance and the metabolic syndrome. We investigated whether manipulating dietary phosphate influences atherogenesis or insulin sensitivity in ...
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Researchers at the University of Miami have identified a new marker to predict progressive kidney failure, death.. They have found that a high level of a hormone that regulates phosphate is associated with an increased risk of kidney failure and death among chronic kidney disease (CKD) patients.. Senior study author Myles Wolf, M.D., M.M.Sc., at the University of Miami, believes this discovery could lead to earlier diagnosis and treatment of phosphate problems.. Treatment typically consists of dietary phosphate restriction and phosphate binders - medications that work like a sponge to soak up phosphate in the gut.. Our bodies need phosphorus to build and repair bones and teeth, help cells function and maintain DNA. With fine-tuned regulation from hormones like FGF23, the kidneys help control the amount of phosphate in the blood by eliminating the excess.. Elevated phosphate levels are often a consequence of advanced kidney disease or damage. But too much phosphate may also make kidney disease ...
associated with effects on many organ systems as well. Because the complications of hyperphosphatemia are not even as predictable as those due to phosphate depletion, phosphate levels are typically described as elevated or severely elevated. Weakness, tetany, seizures, and dysrhythmias, including polymorphic ventricular tachycardia and torsades de pointes due to prolongation of the Q-T interval, can occur with hyperphosphatemia. These effects are not caused directly by the elevated phosphate, but are instead due to the resultant hypocalcemia. The reduction in ionized calcium can be demonstrated by the steady state reaction between calcium and phosphate. Hypomagnesemia, hypernatremia, and metabolic acidosis are also associated with hyperphosphatemia.. Treatment. Oral therapy is preferred over the IV route based on safety, no need for subsequent monitoring of electrolytes, and ease of administration. The plasma phosphate level peaks 1.5 h post ingestion, and typically rises 1 mg/dl for a 1g dose ...
Here, we present a new in situ microfluidic phosphate sensor that features an improved phosphate blue assay which includes polyvinylpyrrolidone in place of traditional surfactants-improving sensitivity and reducing temperature effects. The sensor features greater power economy and analytical performance relative to commercially available alternatives, with a mean power consumption of 1.8 W, a detection limit of 40 nM, a dynamic range of 0.14-10 μM, and an infield accuracy of 4 ± 4.5%. During field testing, the sensor was continuously deployed for 9 weeks in a chalk stream, revealing complex relations between flow rates and phosphate concentration that suggest changing dominance in phosphate sources. A distinct diel phosphorus signal was observed under low flow conditions, highlighting the ability of the sensor to decouple geochemical and biotic effects on phosphate dynamics in fluvial environments. This paper highlights the importance of high resolution in situ sensors in addressing the ...
We have developed methods that yield estimates of the 32P content of each of the individual phosphate groups of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate, thus extending the information available from studies of the labelling of these lipids in intact cells or membrane preparations. The analyses are undertaken with the deacylated lipids. Assay of the 5-phosphate of phosphatidylinositol 4,5-bisphosphate is achieved by the use, under conditions of first-order kinetics, of a 5-phosphate-specific phosphomonoesterase present in isolated erythrocyte membranes [Downes, Mussat & Michell (1982) Biochem. J. 203, 169-177]. Assay of the 4-phosphate of phosphatidylinositol 4-phosphate and of the total monoester phosphate content (4-phosphate plus 5-phosphate) of phosphatidylinositol 4,5-bisphosphate employs alkaline phosphatase from bovine intestine. The radioactivity of the 1-phosphate is that remaining as organic phosphate after exhaustive alkaline phosphatase treatment. ...
TY - JOUR. T1 - Phosphate uptake by TiO2. T2 - Batch studies and NMR spectroscopic evidence for multisite adsorption. AU - Kang, Sue A.. AU - Li, Wei. AU - Lee, Hyo Eun. AU - Phillips, Brian L.. AU - Lee, Young Jae. PY - 2011/12/15. Y1 - 2011/12/15. N2 - Systematic studies, combining batch experiments with NMR spectroscopic methods, are carried out for phosphate sorption on titanium dioxide (TiO2). It is found that phosphate sorption on TiO2 decreases with increasing pH, whereas the phosphate uptake by TiO2 increases with increasing ionic strength of the solution. In I≤0.1M, the sorption sharply increases and reaches a near maximum and then followed by little changes showing Langmuir-type behavior, whereas in I=0.7M, non-Langmuirian uptake becomes evident as equilibrium phosphate concentrations increase in solution. The sorption of phosphate on TiO2 is rapid and mostly irreversible at pH 4.5 and 7.0. At pH 9.0, however, the phosphate sorption is initially reversible and followed by resorption ...
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Cellular metabolism depends on the appropriate concentration of intracellular inorganic phosphate (Pi). Pi starvation-responsive genes appear to be involved in multiple metabolic pathways, implying a complex Pi regulation system in microorganisms and plants. A group of enzymes is required for absorption and maintenance of adequate phosphate levels, which is released from phosphate esters and anhydrides. The phosphatase system is particularly suited for the study of regulatory mechanisms because phosphatase activity is easily measured using specific methods and the difference between the repressed and derepressed levels of phosphatase activity is easily detected. This paper analyzes the protein phosphatase system induced during phosphate starvation in different organisms.
Phosphorus In the body, almost all phosphorus is combined with oxygen to form phosphate. Phosphate is used as a building block for many substances such as DNA, cell membranes, etc. The body obtains phosphate from food and excretes it in urine and sometimes stool. Foods that are phosphate rich are milk, egg yolks, chocolate and soft drinks. Imbalances in phosphate may be too high (Hyperphosphatemia) or too low (Hypophosphatemia).. Instructions for Hyperphosphatemia. You have been diagnosed with hyperphosphatemia, which means you have too much phosphorus in your blood. Phosphorus helps develop bones and teeth and helps control energy metabolism. Most cases of hyperphosphatemia are caused by other health problems, such as kidney disease.. While in the hospital, you may have been treated with medicines for hyperphophatemia.. Diet changes. ...
TY - JOUR. T1 - Roles of phosphate and fibroblast growth factor 23 in cardiovascular disease. AU - Scialla, Julia J.. AU - Wolf, Myles. PY - 2014/4/1. Y1 - 2014/4/1. N2 - Disturbances in phosphate homeostasis are common in patients with chronic kidney disease. As kidney function declines, circulating concentrations of phosphate and the phosphate-regulatory hormone, fibroblast growth factor (FGF)-23, rise progressively. Higher serum levels of phosphate and FGF-23 are associated with an increased risk of adverse outcomes, including all-cause mortality and cardiovascular events. The associations between higher FGF-23 levels and adverse cardiovascular outcomes are generally independent of serum phosphate levels, and might be strongest for congestive heart failure. Higher serum phosphate levels are also modestly associated with an increased risk of cardiovascular events even after accounting for FGF-23 levels. This observation suggests that FGF-23 and phosphate might promote distinct mechanisms of ...
Phosphorus, acquired in the form of phosphate (Pi), is one of the primary macronutrients for plants but is least available in the soil. Pi deficiency is a major factor limiting plant growth, development and reproduction. Plants have developed a complex signaling network to respond to Pi deficiency. The recent discovery of strigolactones, a new class of plant hormones, has led to an emerging signaling module illustrating the integrated control of Pi acquisition, plant-microbe symbiotic interactions and plant architecture. This review article focuses on the recent findings of plant responses and roles of strigolactones to Pi deficiency.
Project approach and work plan:. Phase 1: In the week prior to the collection of standard pre-clinic blood work (to include calcium, phosphate, PTH), PD patients will keep a 3-day diary that will include all foods and beverages consumed in addition to the portion sizes. At the time of standard pre-clinic bloodwork collection, patients will perform an adequest. Medication lists will be reviewed for: 1) Proton pump inhibitors/ H2 receptor antagonists 2) 25-hydroxyvitamin D3 and 1,25 dihydroxyvitamin D3 and 3) phosphate binders. Doses taken the week prior to standard pre-clinic blood work when the diet diaries are compiled will be recorded. This information will be used for 2 separate purposes: 1) A list of the foods that are actually being eaten by the PD patients will be generated 2) The 3-day average phosphate content will be estimated from each PD patients food diaries. A regression equation that predicts serum phosphate concentration taking into account phosphate intake, the amount of ...
Regulation of phosphate homeostasis is critical for many biological processes, and both hypophosphatemia and hyperphosphatemia can have adverse clinical consequences. Only a very small percentage (1%)
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Antibody Array Kit for studying Akt1/Akt1 (Ser473) phosphate/Akt2/Akt2 (Ser474) phosphate/Akt3/Akt3 (Ser472) phosphate/Casp7/Casp7 (Asp198, 20kD) cleaved/ERK1/ERK1 (Thr202/Tyr204) phosphate/ERK2/ERK2 (Thr185/Tyr187) phosphate/Fyn (Tyr420) phosphate/Hck (Tyr411) phosphate/IkB-a/IkB-a (Ser32/Ser36) phosphate/IRF-3/Lck/Lck (Tyr394) phosphate/Lyn (Tyr397) phosphate/p38-alpha MAPK/p38-alpha MAPK (Thr180/Tyr182) phosphate/p38-beta MAPK (Thr180/Tyr182) phosphate/p38-delta MAPK (Thr180/Tyr182) phosphate/p38-gamma MAPK (Thr183/Tyr185) phosphate/RIG-I/SAPK/JNK1/SAPK/JNK1 (Thr183/Tyr185) phosphate/Src (Tyr419) phosphate/Stat1/Stat1 (Ser727) phosphate/Stat1 (Tyr701) phosphate/STAT3/STAT3 (Ser727) phosphate/STAT3 (Tyr705) phosphate/STAT5A/STAT5A (Tyr694) phosphate/STAT5B (Tyr699) phosphate/Stat6/Stat6 (Tyr641) phosphate/Syk/Syk (Tyr352) phosphate/TAK1/TAK1 (Ser439) phosphate/Yes (Tyr426) phosphate/ZAP70/ZAP70 (Tyr319) phosphate in the Screening Technologies research area.
Antibody Array Kit for studying Akt1/Akt1 (Ser473) phosphate/Akt2/Akt2 (Ser474) phosphate/Akt3/Akt3 (Ser472) phosphate/Casp7/Casp7 (Asp198, 20kD) cleaved/ERK1/ERK1 (Thr202/Tyr204) phosphate/ERK2/ERK2 (Thr185/Tyr187) phosphate/Fyn (Tyr420) phosphate/Hck (Tyr411) phosphate/IkB-a/IkB-a (Ser32/Ser36) phosphate/IRF-3/Lck/Lck (Tyr394) phosphate/Lyn (Tyr397) phosphate/p38-alpha MAPK/p38-alpha MAPK (Thr180/Tyr182) phosphate/p38-beta MAPK (Thr180/Tyr182) phosphate/p38-delta MAPK (Thr180/Tyr182) phosphate/p38-gamma MAPK (Thr183/Tyr185) phosphate/RIG-I/SAPK/JNK1/SAPK/JNK1 (Thr183/Tyr185) phosphate/Src (Tyr419) phosphate/Stat1/Stat1 (Ser727) phosphate/Stat1 (Tyr701) phosphate/STAT3/STAT3 (Ser727) phosphate/STAT3 (Tyr705) phosphate/STAT5A/STAT5A (Tyr694) phosphate/STAT5B (Tyr699) phosphate/Stat6/Stat6 (Tyr641) phosphate/Syk/Syk (Tyr352) phosphate/TAK1/TAK1 (Ser439) phosphate/Yes (Tyr426) phosphate/ZAP70/ZAP70 (Tyr319) phosphate in the Screening Technologies research area.
wrote: Hi there, Does anyone know advantages and disadvantages of using potassium phosphate buffer instead of sodium phosphate buffer on the rinsing of IHQ slides? Let me explain... we used to use sodium phosphate mono and dibasic with NaCL, now we are using potassium phosphate mono and sodium phosphate dibasic with NaCL.. Tks! Ms. K tia Catunda Produ o +55 12 3203-0612 (direto) +55 12 3203-0633 (PABX) www.cipax.com.br [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Fussy? Opinionated? Impossible to please? Perfect. Join Yahoo!s user panel and lay it on us. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ...
Contributes a negative charge to the molecule of which it is a part (-2 when at the end of a molecule, -1 when located internally in a chain of phosphates). Biologydictionary.net, November 04, 2016. https://biologydictionary.net/phosphate-group/. Adenosine triphosphate is the organic molecule which acts as a fuel for many biological and biochemical processes to occur, where it releases energy in the form of phosphate as a result of hydrolysis. Which of these activities involves phosphate groups? DNA is composed of individual units, called nucleotides. ribose, uracil, and a phosphate group deoxyribose, uracil, and a phosphate group deoxyribose, thymine, and a phosphate group ribose, thymine, and a phosphate group The molar mass of the phosphate ion is 94.97 g/mol. Retrieved from https://biologydictionary.net/phosphate-group/. A phosphate group, in the context of biology, is a molecule composed of a phosphorous atom and four oxygen atoms. Biology Biology 2e A nucleotide of DNA may contain. ADP ...
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high quality Sodium Hexametaphosphate CAS No.: 10124 -56- 8 Other Names: SHMP MF: (NaPO3)6 EINECS No.: 233-343-1 Place of Origin: Beijing China (Mainland) Grade Stand
The HI93713-01 is a reagent for the determination of kolorimetri phosphate. There are enough reagents for 100 tests for use with compatible Hanna benchtop P257292
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TY - JOUR. T1 - Structure and function of the GTP binding protein Gtr1 and its role in phosphate transport in Saccharomyces cerevisiae. AU - Lagerstedt, Jens O.. AU - Reeve, Ian. AU - Voss, John C. AU - Persson, Bengt L.. PY - 2005/1/18. Y1 - 2005/1/18. N2 - The Pho84 high-affinity phosphate permease is the primary phosphate transporter in the yeast Saccharomyces cerevisiae under phosphate-limiting conditions. The soluble G protein, Gtr1, has previously been suggested to be involved in the derepressible Pho84 phosphate uptake function. This idea was based on a displayed deletion phenotype of Δgtr1 similar to the Δpho84 phenotype. As of yet, the mode of interaction has not been described. The consequences of a deletion of gtr1 on in vivo Pho84 expression, trafficking and activity, and extracellular phosphatase activity were analyzed in strains synthesizing either Pho84-green fluorescent protein or Pho84-myc chimeras. The studies revealed a delayed response in Pho84-mediated phosphate uptake and ...
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Functions: Emulsifier, Humectant, Sequestrant, Stabilizer, Thickener. Calcium phosphate is a family of materials and minerals containing calcium ions -Ca2+- together with inorganic phosphate anions. Some so-called calcium phosphates contain oxide and hydroxide as well. They are white solids of nutritious value. - Wikipedia. Names: Phosphate de calcium dhydrogène, Phosphate de calcium dihydrogène, Phosphates de calcium, Orthophosphates de calcium, Orthophosphate monocalcique, phosphate monocalcique, Orthophosphate dicalcique, Orthophosphate tricalcique, Phosphate tricalcique, Phosphate de calcium monobasique, Phosphate de calcium dibasique, Phosphate de calcium tribasique, Phosphate de calcium (i), Phosphate de calcium (ii), Phosphate de calcium (iii), calcium (phosphate), phosphate de calcium. ...
ROWAphos GFO Phosphate Removal Media, 100 ml. - At AquaCave, we offer Best Prices, 5% Back, and Free Shipping on ROWAphos GFO Phosphate Removal Media, 100 ml. . - Buy ROWAphos GFO Phosphate Removal Media, 100 ml. - Now Only $13.95 - ROWAphos Phosphate Removal Media, 100 ml.ROWA®phos is a completely new filter material for eliminating phosphate, which was developed by the Weil Industrieanlagen GmbH company in Osnabrück, Germany. Due to its unique chemistry, it has the ability to bind large quantities of phosphate by adsorption to its surface without negatively influencing the water in the aquarium in any way. ROWAphos is the professional method of removing Phosphate and Silicate in all types of Aquariums and Ponds. It has the largest absorption ability of any phosphate product and will not leach back in to the water. ROWAphos is the most effective phosphate remover on the market today. It is widely used in Europe and is now finding success in the United States. ROWAphos was developed in
In this study, we cloned and characterized a bHLH transcription factor, OsPTF1, which is responsible for tolerance to Pi starvation in rice. Overexpression of OsPTF1 can enhance tolerance to Pi deficiency. It was reported that PHO4, which was also a bHLH protein, acts as a key regulator of PHO regulon in yeast. In Pi starvation conditions, PHO4 combines with its partner, PHO2, and induces the expression of PHO5 and PHO84, the Pi starvation-induced acid phosphatase and the high-affinity Pi transporter (Johnston and Carlson, 1992; Oshima, 1997). Microarray analysis was performed to test whether OsPTF1 regulates downstream genes as PHO4. Unexpectedly, no high-affinity phosphate transporter genes or acid phosphatase genes were found to be controlled by OsPTF1 (see supplemental data). In addition, OsPTF1 is located in the nucleus in both high- and low-Pi conditions, which is different from PHO4, which is imported into or exported out of the nucleus under Pi-sufficient or starvation conditions (Kaman ...
The increase in the ratio of root growth to shoot growth that occurs in response to phosphate (Pi) deprivation is paralleled by a decrease in cytokinin levels under the same conditions. However, the role of cytokinin in the rescue system for Pi starvation remains largely unknown. We have isolated a gene from Arabidopsis thaliana (AtIPS1) that is induced by Pi starvation, and studied the effect of cytokinin on its expression in response to Pi deprivation. AtIPS1 belongs to the TPSI1/Mt4 family, the members of which are specifically induced by Pi starvation, and the RNAs of which contain only short, non-conserved open reading frames. Pi deprivation induces AtIPS1 expression in all cells of wild-type plants, whereas in the pho1 mutant grown on Pi-rich soils, AtIPS1 expression in the root was delimited by the endodermis. This supports the view that pho1 is impaired in xylem loading of Pi, and that long-distance signals controlling the Pi starvation responses act via negative control. Exogenous cytokinins
Citric acid and sodium hexametaphosphate are often used in processed cheese as emulsifier. These kinds of salts improve the proteins swelling capacity and emulsification and thus inhibits the leakage of water or fat from the product (forms metal complexes). Some salts are also acid buffers. 1 In this wikipedia article (in German, but chemical names are quite similiar to the Engish ones) is a list of possible substitutes of citric acid and sodium hexametaphosphate. Or just look here (in English) for E 325, E 326, E 327, E 331, E 332, E 333, E 339, E 340, E 341, E 450, E 451, E 452. The receipe of the cheese sauce has the same ingredients of common processed cheese2. ...
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Maize (Zea mays) is not only a key human food and animal feed crop throughout the world but also an important raw material for the food industry and energy production plants [1]. Low phosphate concentrations are frequently a constraint for maize growth and development, and therefore, enormous quantities of phosphate fertilizer are expended in maize cultivation, which increases the cost of planting. Although the total amount of phosphorus (P) in the soil may be high, plants mainly absorb P in the inorganic form (Pi), which is present at a low concentration, limiting plant growth and development [2].. Phosphorus is an essential macronutrient for all living organisms and plays important roles in energy metabolism; biosynthesis of nucleic acids, phospholipids and membranes; cellular signal transduction and the regulation of many enzymes [3, 4]. Plants have evolved two broad strategies to cope with phosphate starvation, which involve changes in physiology, biochemistry and root morphology that ...
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TY - JOUR. T1 - Effective phosphate removal from synthesized wastewater using copper-chitosan bead. T2 - Batch and fixed-bed column studies. AU - An, Byungryul. AU - Jung, Ka Young. AU - Lee, Sang-Hyup. AU - Lee, Seunghak. AU - Choi, Jae Woo. PY - 2014. Y1 - 2014. N2 - To remove phosphate from solution, a new class of sorbent based on chitosan bead (CB) was prepared using copper ion (Cu(II)) with/without a traditional crosslinking agent (glutaraldehyde [GLA]); these materials are referred to as CB-G-Cu and CB-Cu, respectively. Copper ions play a key role in the CB synthesis; these species crosslink each polymer chain, and during phosphate removal, they are the active functional group. Overall, 2.5% (w/w) of chitosan is necessary to maintain the physical properties of the bead. In the FTIR spectra, adding GLA decreased the intensity of the amino group in chitosan, lowering the amount of copper in the CB. The maximum phosphate uptake (Q) for CB-Cu was 53.6 mg g-1 when calculated with the Langmuir ...
The mechanisms and regulation of intestinal phosphate absorption remain poorly defined. This is partly due to known species differences, as well as the variety of techniques and experimental conditions that have been used to assess phosphate absorption, particularly the wide range (and largely unphysiological choice) of phosphate concentrations added to uptake buffers. In the present study we have determined the luminal concentration of phosphate available for absorption, and we have used in vivo and in vitro methods to characterize the relative contributions of Na+‐dependent and Na+‐independent phosphate transport along the rat small and large intestine.. We have used the in vitro, everted sleeve technique developed by Karasov and Diamond (Karasov et al. 1983), and optimized by ourselves for the measurement of phosphate transport at the enterocyte BBM (Marks et al. 2007). Phosphate uptake was measured within the linear phase to ensure unidirectional flux, and that the isotope had not ...
JM Land, DJ Taylor, GJ Kemp, GK Radda, B Rajagopalan; Idiopathic Renal Hypophosphataemia: Oral Phosphate Reverses Abnormalities in Muscle. Clin Sci (Lond) 1 March 1992; 82 (s26): 26P. doi: https://doi.org/10.1042/cs082026Pa. Download citation file:. ...
Sucroferric oxyhydroxide (INN; trade name Velphoro, by Vifor Fresenius Medical Care Renal Pharma) is a non-calcium, iron-based phosphate binder used for the control of serum phosphorus levels in adult patients with chronic kidney disease (CKD) on haemodialysis (HD) or peritoneal dialysis (PD). It is used in form of chewable tablets. In a healthy person, normal serum phosphate levels are maintained by the regulation of dietary absorption, bone formation and resorption, equilibration with intracellular stores, and renal excretion. When kidney function is impaired, phosphate excretion declines. Without specific treatment, hyperphosphataemia occurs almost universally, despite dietary phosphate restriction and conventional dialysis treatment. In patients on dialysis, hyperphosphataemia is an independent risk factor for fractures, cardiovascular disease and mortality. Abnormalities in phosphate metabolism such as hyperphosphatemia are included in the definition of the new chronic kidney ...
Fritz Phosphate Control is a new liquid additive which is designed to quickly remove phosphates from aquarium water. Using Lanthanum as a primary ingredient…
Experimental. Reagent material. Methanol, dichloromethane, ethanol, acetic acid were HPLC grade; disodium hydrogen phosphate, sodium dihydrogen phosphate were of analytical grade; ultra-pure water; blood;. Acetic acid - solution: Take 50 mL of deionized water with acetic acid adjusted to pH = 6;. Disodium hydrogen phosphate solution: 0.1 mol/L, weighed 35.81 g disodium hydrogen phosphate (Na2HPO4 • 12H2O), dissolved in water and dilute to 1 L;. Sodium dihydrogen phosphate solution: 0.1 mol/L, weighed 15.60 g disodium hydrogen phosphate (Na2HPO4 • 12H2O), dissolved in water and dilute to 1 L;. 0.1 mol/L phosphate buffer solution (pH = 6): The 1.70 g of dibasic sodium phosphate and 12.14 g sodium dihydrogen phosphate was dissolved in 800 mL of deionized water, and then diluted with deionized water to 1 L, mix well. pH adjusted to 6.0 (with 0.1 mol/L sodium dihydrogen phosphate to lower pH; with 0.1 mol/L disodium hydrogen phosphate to increase the pH);. Hypnotic standard stock solution: mixed ...
DESCRIPTION (provided by applicant): In patients with chronic kidney disease (CKD), phosphate retention may contribute to progression of renal failure and is a major factor in the development of secondary hyperparathyroidism and vascular calcification. Progression of injury is causally associated with risk of mortality that is estimated 10 to 30 times higher for CKD patients undergoing dialysis than the general population. In fact, death is a more common outcome than dialysis or transplantation in patients with CKD. Phosphate binders are clinical mainstays in reducing dietary phosphate absorption and preventing hyperphosphatemia in the roughly 370,000 end-stage renal disease (ESRD) patients in the United States. While medicines exist that bind phosphorus effectively, all current forms of phosphate binder therapy are associated with very poor patient compliance due to a host of problems, including side effects that run the gamut of poor taste, halitosis, gastric bloating, constipation, the number ...
Hyperphosphatemia, that is, an abnormally high serum phosphate level, can result from increased phosphate (PO4) intake, decreased phosphate excretion, or a disorder that shifts intracellular phosphate to extracellular space. (See Pathophysiology, Etiology, Clinical Presentation, and Workup.
Introduction: Serum phosphate levels are insufficiently controlled in many patients with end-stage renal disease (ESRD), and novel therapeutic strategies are needed. Blocking intestinal phosphate absorption mediated by sodium-dependent phosphate co-transporter type 2b (NPT-IIb) holds promise; thus, we evaluated the efficacy, safety, tolerability, and pharmacokinetics of the novel and specific small molecule NPT-IIb inhibitor ASP3325 for the first time in humans. Methods: We conducted a randomized, double-blind, placebo-controlled, phase 1a single (n = 88) and multiple (n = 36) ascending dose study in healthy subjects, and a randomized, open-label, uncontrolled, phase 1b study in hyperphosphatemic ESRD patients on hemodialysis (single oral dose, n = 5; multiple oral doses, n = 17 ...
In order to determine the relation between depression of myocardial function and the myocardial high energy phosphate stores during acute hypoxia, a technique for serial evaluation of these stores was developed. Acute hypoxia was induced in 18 anesthetized dogs and serial determinations of adenosine triphosphate (ATP) and creatine phosphate (CP) were made while myocardial function was continuously measured. Myocardial concentrations of ATP were maintained even in the presence of severe hypoxia and myocardial failure. There was a small depression of the average myocardial CP concentration during early heart failure. However, in more than one-third of the dogs there was no significant depression of CP concentration at this time. It is concluded that hypoxic depression of myocardial function is not initiated by a decrease in the total myocardial high energy phosphate stores. The possibility could not be excluded that a particular fraction of the high energy phosphate store is decreased when ...
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Espoma company gardening rock phosphate. soil did test low in potash, and phosphate, using 40 amended coco coir e.g., will not affect soil ph mixed with regular dirt from the yard, and understand that quotrockquot phosphate is highly recommended for a vegetable garden.. The world of rock phosphate of all types and grades, catering the agricultural needs of 16th population of the world. out of the total rock phosphate resource, the country has a predominance of low grade rock phosphate having only 15.27 million tones reserve of high grade rock phosphate table 1, and the remaining low grade rock phosphate is. Phosphate rock an overview sciencedirect topics. swapan kumar haldar, in mineral exploration second edition, 2018. 13.5.7.5 flotation of rock phosphate ore. rock phosphate, the primary raw material for fertilizer and phosphoric acid, occurs as highgrade ore 30 p 2 o 5, mediumgrade ore 2030 p 2 o 5, and lowgrade ore 1520 p 2 o 5.the very highgrade ore blends with .... Bond amp stock ratings ...
Microorganisms that release plant-available phosphate from natural soil phosphate stores may serve as biological alternatives to costly and environmentally damaging phosphate fertilizers. To explore this possibility, we engineered a collection of root bacteria to release plant available orthophosphate from phytate, an abundant phosphate source in many soils. We identified 82 phylogenetically diverse phytase genes, refactored their sequences for optimal expression in Proteobacteria, then synthesized and engineered them into the genomes of three root-colonizing bacteria. Liquid culture assays revealed 41 engineered strains with high levels of phytate hydrolysis. Among these, we identified 12 strains across three bacterial hosts that confer a growth advantage on the model plant Arabidopsis thaliana when phytate is the sole phosphate source. These data demonstrate that DNA synthesis approaches can be used to generate plant-associated strains with novel phosphate solubilizing capabilities.Importance ...
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We report the synthesis of luminescent nanoparticles of manganese doped zinc sulfide (ZnS:Mn2+) with an emission peak at around 590 nm. Nanoparticles of ZnS:Mn2+ are prepared by a co-precipitation reaction from homogenous solutions of zinc and manganese salts. Based on Ostwald ripening and surface passivation, we discuss a mechanism for the formation of ZnS:Mn2+ nanoparticles. The reaction proceeds with the nucleation of ZnS crystals, which are immediately passivated by the anions in the solution. This in turn attracts cations including zinc and manganese which contribute to the growth of the crystal. These nanoparticles are sterically stabilized using polyphosphates of sodium namely sodium tripolyphosphate (STTP) and sodium hexametaphosphate (SHMP). The nanoparticles consist of particles of 60-80 nm in diameter, each containing primary crystallites that was estimated from the X-ray diffraction patterns to be at around 2.2 nm. ...
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Genotypic variability in utilization of rock phosphates including the local source Eppawale was evaluated using Hevea clones during the early immature phase. All the clones responded to P fertilization, and the responces were related to the source of fertilizers and other soil characteristics. Plants of clone RR1C 121 utilized Eppawala rock phosphate efficiently, both in Matale and Boralu soil. But, plants of clone RR1C 110 were able to use both rock phosphate sources only in Matale soils. However, responce to rock phosphate from plants of 100 was comparatively low in relation to P uptake and dry matter production. The necessity of testing these clones for the locally available phosphate source in the field scale is also ...
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Phosphate is a chemical compound that contains phosphorus and oxygen. It is necessary to the growth of plants and animals, and has extensive use as fertilizers. Phosphates are also used in the manufacture of detergents. In these products, phosphates help remove dirt and soften hard water. There are large amounts of natural phosphates. They occur in phosphate rocks, mostly combined with the elements calcium and magnesium. They also occur in the remains of animals(bone ash) and of plants (vegetable mold). Phosphate rock is the chief source of fertilizers containing phosphates. A soluble fertilizer known as superphosphate acts much more quickly than the pulverized rock. It is made by crushing the phosphate rock and treating it with sulfuric acid. Phosphorous is a chemical element that man, animals, and plants need for normal growth. Phosphorus is also used in the manufacture of many industrial products, such as plant fertilizers. Plants absorb phosphorus compounds from the soil, and human beings ...
ATP-binding cassette domain of the phosphate transport system. Phosphate uptake is of fundamental importance in the cell physiology of bacteria because phosphate is required as a nutrient. The Pst system of E. coli comprises four distinct subunits encoded by the pstS, pstA, pstB, and pstC genes. The PstS protein is a phosphate-binding protein located in the periplasmic space. PstA and PstC are hydrophobic and they form the transmembrane portion of the Pst system. PstB is the catalytic subunit, which couples the energy of ATP hydrolysis to the import of phosphate across cellular membranes through the Pst system, often referred as ABC-protein. PstB belongs to one of the largest superfamilies of proteins characterized by a highly conserved adenosine triphosphate (ATP) binding cassette (ABC), which is also a nucleotide binding domain (NBD). ...
The motor protein kinesin couples a temporally periodic chemical cycle (the hydrolysis of ATP) to a spatially periodic mechanical cycle (movement along a microtubule). To distinguish between different models of such chemical-to-mechanical coupling, we measured the speed of movement of conventional kinesin along microtubules in in vitro motility assays over a wide range of substrate (ATP) and product (ADP and inorganic phosphate) concentrations. In the presence and absence of products, the dependence of speed on [ATP] was well described by the Michaelis-Menten equation. In the absence of products, the K(M) (the [ATP] required for half-maximal speed) was 28 +/- 1 microM, and the maximum speed was 904 nm/s. P(i) behaved as a competitive inhibitor with K(I) = 9 +/- 1 mM. ADP behaved approximately as a competitive inhibitor with K(I) = 35 +/- 2 microM. The data were compared to four-state kinetic models in which changes in nucleotide state are coupled to chemical and/or mechanical changes. We found ...
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A 74-year-old woman presented with a 2-day history of abdominal pain and nausea, 1 week after undergoing upper and lower endoscopies. Her comorbidities included hypertension, dyslipidaemia, sigmoid diverticulosis, transient ischaemic attack, peripheral vascular disease and mild valvular heart disease.. Medications taken before admission included telmisartan-hydrochlorothiazide, sustained-release verapamil, prazosin, rosuvastatin and aspirin.. Admission blood tests indicated acute kidney injury, with a serum creatinine concentration of 218 μmol/L (reference interval [RI], 46-99 μmol/L) and an estimated glomerular filtration rate of 19 mL/min/1.73 m2 (RI, , 60 mL/min/1.73 m2); her creatinine level 7 months earlier was 69 μmol/L. She was also hypokalaemic (potassium level, 2.9 mmol/L [RI, 3.5-5.1 mmol/L]), hypocalcaemic (corrected calcium concentration, 1.97 mmol/L [RI, 2.15-2.6 mmol/L]) and uraemic (urea concentration, 14.4 mmol/L [RI, 2.9-8.2 mmol/L]). However, she was normophosphataemic ...
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In this study, the efficient phosphate utilizing isolates were used to remove phosphate from synthetic phosphate wastewater was tested using batch scale process. Hence the objective of the present study was to examine the efficiency of bacterial species individually for the removal of phosphate from synthetic phosphate wastewater. The most efficient phosphate reducers were isolated and screened from eutrophic lake water samples. The total heterotrophic bacterial analysis of the samples showed the presence of about 22 phosphate reducers. Among them, Pseudomonas sp YLW-7 were found to be efficient in phosphate reduction based on the maximum phosphate ultization which was observed by plate screening method using the minimum inhibitory concentration (MIC) test. The effect of carbon sources (glucose, starch, sucrose and lactose) at 0.5% on the removal of phosphate by Pseudomonas sp YLW7 was estimated. The maximum growth of Pseudomonas sp YLW7 was observed to be 0.9886 OD in glucose followed by starch ...
Barak, A J.; Allison, T B.; and Sorrell, M F., Relationship of selenium to high energy phosphate compounds in muscle of dystrophic mice. (1974). Subject Strain Bibliography 1974. 2353 ...
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