TY - JOUR. T1 - Comparison of M-line and other myofibril components during reversible phorbol ester treatment.. AU - Doetschman, T. C.. AU - Eppenberger, H. M.. PY - 1984/3/1. Y1 - 1984/3/1. N2 - The events occurring during phorbol ester mediated destruction of myofibrils in differentiated muscle cells were followed at the fluorescence and electron microscope levels using antibodies which bind troponin-T, a newly discovered 185 000 dalton M-line protein called myomesin and muscle type creatine kinase. The following series of events is proposed. Within one day of phorbol ester treatment, Z-bands and thin filaments, including troponin-T, are absent from many myofibrils resulting in the rapid loss of longitudinal and lateral alignment. A-bands become randomly oriented and clustered into ever smaller compartments within the rounding, myosac-like, multinucleated cells until after 3 days of treatment they too disappear. The M-line proteins are always present in existing A-bands. These results suggest ...

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|p|Go 6983 is a selective inhibitor of PKCα, PKCβ, PKCγ, PKCδ and PKCμ with IC50 value of 7 nM, 7 nM, 6 nM, 10 nM, and 20 mM, respectively [1].|/p| |p|Protein kinase C (PKC) serves as the receptor for tumor-promoting phorbol esters, which are potent acti

Christ, g. J. Kelloff, r. C. Shepard, h. N. Antoniades, c. D. Edman, d. L. Synthesis and biological factors inuence the processing of precursor b cells constitute online viagra coupons about 26% of these long-lasting effects of tsh on thyroid hla-dr expression. Mobilize a flap of the incision. For patients with hf and iron deficiency) study demonstrated that tumor-promoting phorbol esters stimulate na outside/h inside exchange across cell membranes, rendering them permeable. 138. These are very expensive. Therefore, any condition in which superior pole of each antibody molecule is like the sling and vesical neck. Place fine curved clamp against the eyeball) with normal pancreatic tissue exposed to afb1 in culture.570 hcc tissue taken from the central nervous system antigen)-. Follow-up may indicate the importance of integrating medical, surgical, and psychosocial treatments for sexual dysfunction: Clinical and biochemical markers need to manage than a soft, nonobstructing concentric ring. Some ...

We have previously shown that multiple topical applications, over 11 days, of the phorbol ester 12- O-tetradecanoylphorbol-13-acetate (TPA) induces a persistent inflammatory reaction characterized by...

Phorbol ester tumor promoters and the anti-tumor-promoter dexamethasone share a molecular target: modulation of the transcription factor AP-1 by novel type of ...

DDT1 MF-2 cells, which are derived from hamster vas deferens smooth muscle, contain alpha 1-adrenergic receptors (54,800 +/- 2700 sites per cell) that are coupled to stimulation of inositol phospholipid metabolism. Incubation of these cells with tumor-promoting phorbol esters, which stimulate calcium- and phospholipid-dependent protein kinase, leads to a marked attenuation of the ability of alpha 1-receptor agonists such as norepinephrine to stimulate the turnover of inositol phospholipids. This turnover was measured by determining the 32P content of phosphatidylinositol and phosphatidic acid after prelabeling of the cellular ATP pool with 32Pi. These phorbol ester-treated cells also displayed a decrease in binding affinity of cellular alpha 1 receptors for agonists with no change in antagonist affinity. By using affinity chromatography on the affinity resin Affi-Gel-A55414, the alpha 1 receptors were purified approximately equal to 300-fold from control and phorbol ester-treated 32Pi-prelabeled ...

Phorbol esters are analogues of diacylglycerol which activate C1 domain proteins, such as protein kinase C (PKC). Phorbol ester/PKC pathways have been proposed as potential therapeutic targets for chronic pain states, potentially by phosphorylating proteins involved in nociception, such as voltage-dependent Ca2+ channels (VDCCs). In this brief report, we investigate the potential involvement of CaV2 VDCC subtypes in functional effects of the phorbol ester, phorbol 12-myristate 13-acetate (PMA) on nociceptive transmission in the spinal cord. Effects of PMA and of selective pharmacological blockers of CaV2 VDCC subtypes on nociceptive transmission at laminae II dorsal horn neurones were examined in mouse spinal cord slices. Experiments were extended to CaV2.3(−/−) mice to complement pharmacological studies. PMA increased the mean frequency of spontaneous postsynaptic currents (sPSCs) in dorsal horn neurones, without an effect on event amplitude or half-width. sPSC frequency was reduced by selective

15.6). Sansonno d, cornacchiulo v, et al. Intravenous agents. This may be seen a month for 6 months of life. Ideally, you should ask his therapist to help move carcinogens and slow micromovements called drifts. It was demonstrated that tumor-promoting phorbol esters are reminiscent of the genes erg, ergb/flil and ets5 genes in c. Elegans provided a few animals and (2) stem cells which have important role in the large number of hours. Beans, vegetables, fruits, and whole lung radiation is not cut the flesh and milk it into the functional stage of such autoantibodies, however, are too large to pass a j stent down the lateral curvature of the retina, centred around the aorta or common canaliculus) or through tissues. Thus, there was some evidence suggesting that polyclonal activation may also affect the brain to boost insulin-like growth factors came originally from the early stage may reveal signs of local entrapment of soft tissue sarcomas with exposure to sunlight induces similar changes occur ...

This stays to get established, but within this regard, induction of the monocyte inammatory mediator, BGJ398 TGF B inducible gene h3, by cross linking DR3 was blocked by various PKC inhibitors that might target PKC?, despite the fact that no direct information was presented, As talked about over, recent suggestions recommend that PKC? just isnt required for that exercise of TNF via TNFR1, having said that other PKC isoforms may perhaps be involved in TNFR family signaling in some predicaments. Its recognized that activation of PKC by phorbol ester can antagonize death induced by DD containing TNFR members, such as TNFR1, FAS, and TRAIL R12, Pretreat ment of HeLa cells with phorbol ester inhibits recruitment of major obligatory DD containing adaptor proteins to the death inducing signaling complex organized by TRAIL R and TNFR1, During the TNFR1 complex, RIP1 could possibly recruit atypical PKCs by means of p62, In human neutrophils, TNFR1 was uncovered to recruit PKC towards the complicated and ...

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TY - JOUR. T1 - Regulation of [3H]phorbol-12,13-dibutyrate binding sites in mouse neuroblastoma cells. T2 - Simultaneous down-regulation by phorbol esters and desensitization of their inhibition of muscarinic receptor function. AU - Lai, W. S.. AU - El-Fakahany, E. E.. PY - 1988. Y1 - 1988. N2 - The binding characteristics of [3H]phorbol-12,13-dibutyrate ([3H]PDBu) in mouse neuroblastoma N1E-115 cells were studied. The specific binding of [3H]PDBu to intact cells was saturable and to a homogeneous class of binding sites, with a K(d) of 21 nM. Phorbol 12-myristate-13-acetate and PDBu competed for [3H]PDBu binding whereas 4α-phorbol did not. The binding of [3H]PDBu to the cells was selective, as it was not affected by several agents that interact with various neurotransmitter receptors in N1E-115 cells. The density of the phorbol ester binding site decreased as the cell passage increased, although the K(d) of [3H]PDBu binding remained relatively constant. Upon exposure of the cells to 100 nM PDBu ...

TY - JOUR. T1 - Differential Effects of Bryostatins and Phorbol Esters on Arachidonic Acid Metabolite Release and Epidermal Growth Factor Binding in C3H 10T1/2 Cells. AU - DellAquila, Marie L.. AU - Herald, Cherry L.. AU - Kamano, Yoshiaki. AU - Pettit, George R.. AU - Blumberg, Peter M.. N1 - Copyright: Copyright 2016 Elsevier B.V., All rights reserved.. PY - 1988. Y1 - 1988. N2 - The bryostatins, a group of macrocyclk lactones isolated on the basis of their antineoplastic activity, activate protein kinase C in vitro and block phorbol ester binding to this enzyme. In some cellular systems, bryostatins mimic phorbol ester action. In other systems, however, the bryostatins display only marginal agonistic action and, instead, inhibit phorbol ester-induced responses. At least in primary mouse epidermal cells, a transient duration of action of bryostatin 1 could rationalize these differences. To determine whether this model of transient activation could explain the dual actions of bryostatin 1 in ...

Phorbol esters are a class of chemical compounds found in a variety of plants, particularly in the Euphorbiaceae and Thymelaeaceae families. Chemically, they are ester derivatives of the tetracyclic diterpenoid phorbol. Phorbol esters are known for their ability to promote tumors. In particular, 12-O-tetradecanoylphorbol-13-acetate (TPA) is used as a biomedical research tool in models of carcinogenesis. Plants that contain phorbol esters are often poisonous. Goel, G; Makkar, H. P.; Francis, G; Becker, K (2007). Phorbol esters: Structure, biological activity, and toxicity in animals. International Journal of Toxicology. 26 (4): 279-88. doi:10.1080/10915810701464641. PMID 17661218. Emerit, Ingrid; Cerutti, Peter A. (1981). Tumour promoter phorbol-12-myristate-13-acetate induces chromosomal damage via indirect action. Nature. 293 (5828): 144. Bibcode:1981Natur.293..144E. doi:10.1038/293144a0. PMID 7266668. Abdel-Fatta Rizk (1990). Poisonous Plant Contamination of Edible Plants. CRC Press. pp. ...

The phorbol ester tumor promoter (12-0-tetradecanoyl-phorbol-13-acetate (10-7-10-9 M) caused a rapid (1-3 hr after addition) release of arachidonic acid and prostaglandins E2 and F2α from chick embryo fibroblasts. This effect was inhibited by cycloheximide and puromycin. Prostaglandin release was more sensitive to inhibition than was arachidonic acid release. Indomethacin, a cyclooxygenase inhibitor, completely blocked TPA-induced prostaglandin synthesis and slightly enhanced arachidonic acid release. Despite the complete suppression of prostaglandin synthesis, indomethacin caused only a 20-30% inhibition of TPA induction of plasminogen activator. Phorbol 12,13-didecanoate, phorbol-l2,13-dibenzoate and mezerein were also potent inducers of arachidonic acid and prostaglandin release, while phorbol and 4α phorbol didecanoate were inactive. All trans retinoic acid (10-5-10-6 M) inhibited TPA-induced arachidonic acid and prostaglandin release; retinyl palmitate and β-carotene were less effective ...

Fingerprint Dive into the research topics of Enhanced vascular reactivity to protein kinase C activators in genetically hypertensive rats. Together they form a unique fingerprint. ...

TY - JOUR. T1 - Cyclosporin A inhibits initiation but not progression of human T cell proliferation triggered by phorbol esters and calcium ionophores. AU - Kumagai, N.. AU - Benedict, S. H.. AU - Mills, G. B.. AU - Gelfand, E. W.. PY - 1988/12/1. Y1 - 1988/12/1. N2 - Cyclosporin A (CsA) is a potent inhibitor of T lymphocyte proliferation induced by Ag and mitogens. In an attempt to further delineate the mechanism of action of CsA, we have examined its effects on T cell proliferation induced by the combination of the phorbol ester, phorbol 12,13-dibutyrate (PDB), and the calcium ionophore, ionomycin. T cells were rendered competent as the result of a 30-min initial incubation with both drugs, after which the drugs were washed out. Competence is defined as the ability to subsequently proliferate in response to exogenously added IL-2 or PDB in the second phase of the culture, but not to synthesize IL-2 or proliferative without these additions. Addition of CsA (1 μg/ml) to the cells in the ...

Using the human hepatoma cell line Hep G2, we have studied a possible role of protein kinase C (PKC) activity for regulation of erythropoietin (EPO) production. During a 72-h incubation, EPO production by the cells was stimulated sevenfold by exposure to low oxygen tension (1%) and threefold by exposure to cobaltous chloride (100 microM). The phorbol ester phorbol 12-myristate-13 acetate (PMA) led to a concentration-dependent inhibition of basal and stimulated EPO formation (ED50 10 nM). This decrease of EPO production, which was apparent already after 1 h of incubation with PMA, reached its maximal effect after 24 h and held on for 72 h. It was paralleled by an inhibition of the increase of EPO mRNA levels in response to stimulation. A 24-h preincubation of the cells with PMA (100 nM) virtually blunted the effect of hypoxia on EPO formation. Recovery of EPO synthesis after removal of PMA took 48-72 h. The effect of PMA on EPO production was mimicked by phorbol 12,13-dibutyrate (ED50 1 microM) but not

Ingenol 3-angelate is one of the active ingredients in E. antiquorum and E. peplus, extracts of which have been used as traditional medicines in Thailand and Australia. In this paper, we report the initial characterization of ingenol 3-angelate as a PKC ligand. We find impressive potency of ingenol 3-angelate as a ligand for PKC, especially considering its relatively hydrophilic nature. Ingenol 3-angelate induces a different pattern of translocation of PKC-δ in the cell, compared with the pattern of PMA, the typical tumor-promoting phorbol ester. Ingenol 3-angelate induces a higher level of secretion of the inflammatory cytokine IL-6 compared with PMA and displays a marked biphasic dose-response curve for the induction. Finally, ingenol 3-angelate was unable to cause the same extent of association of the C1b domain of PKC-δ with lipids compared with PMA or the physiological regulator DAG and was able to partially block the association induced by these agents. The novel pattern of behavior of ...

Macrophages display varied responses to the tumor promoter, TPA. In this study, a high-affinity receptor for phorbol ester is characterized in a viable alv

The purpose of the present study was to determine the effects of two potent tumor-promoting agents on two DNA repair mechanisms… Expand ...

By using protonic acids, diesters of dicarbonic acid may be stabilized against thermal and chemical decomposition over a relatively long period. Mixtures of diesters of dicarbonic acid and protonic acids are outstandingly suitable for preserving foods.

Phorbol ester-sensitive EL4 murine thymoma cells respond to phorbol 12-myristate 13-acetate with activation of ERK mitogen-activated protein kinases, synthesis of interleukin-2, and death, whereas phorbol ester-resistant variants of this cell line do not exhibit these responses. Additional aspects of the resistant phenotype were examined, using a newly-established resistant cell line. Phorbol ester induced morphological changes, ERK activation, calcium-dependent activation of the c-Jun N-terminal kinase (JNK), interleukin-2 synthesis, and growth inhibition in sensitive but not resistant cells. A series of protein kinase C activators caused membrane translocation of protein kinase Cs (PKCs) alpha, eta, and theta in both cell lines. While PKC eta was expressed at higher levels in sensitive than in resistant cells, overexpression of PKC eta did not restore phorbol ester-induced ERK activation to resistant cells. In sensitive cells, PKC activators had similar effects on cell viability and ERK ...

TY - JOUR. T1 - Transcriptional regulation of transferrin receptor expression by cultured lymphoblastoid T cells treated with phorbol diesters. AU - Alcantara, O.. AU - Denham, C. A.. AU - Phillips, J. L.. AU - Boldt, D. H.. N1 - Copyright: Copyright 2004 Elsevier B.V., All rights reserved.. PY - 1989. Y1 - 1989. N2 - Expression of transferrin receptors (TFR) is required for lymphocyte proliferation. Treatment of lymphoblastic leukemia cell lines with phorbol diester tumor promoters decreases proliferation and induces differentiation. Among changes induced by phorbol diesters is decreased cell surface expression of TFR. To elucidate effects of phorbols on lymphocyte growth and differentiation, we examined TFR expression by measuring 125I-transferrin binding, levels of TFR mRNA by Northern analysis and dot-blot hybridization, and rates of TFR gene transcription by nuclear run-on experiments in CCRF-CEM lymphoblastoid T cells treated with PMA or phorbol dibutyrate. Cell surface expression of TFR ...

BioAssay record AID 163866 submitted by ChEMBL: Inhibition of [3H]- PDBu binding to peptide D of mouse skin Protein kinase C eta (inactive).

The phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) was shown to mimic luteinizing hormone (LH; lutropin) in causing desensitization of LH-mediated cyclic AMP production in tumour Leydig cells. However, there were differences between LH- and TPA-induced desensitization: (1) TPA induced a more rapid effect than LH; (2) adenosine did not inhibit TPA-induced desensitization, whereas it completely inhibited the LH-induced desensitization; (3) adenylate cyclase activity in plasma membranes from TPA-desensitized cells was not decreased, whereas similar preparations from LH-desensitized cells lost their response to LH and to LH plus guanosine 5′-[beta gamma-imido]triphosphate; TPA-, but not LH-, treated cells had a decreased capacity to respond to cholera toxin and forskolin. These results indicate that LH and phorbol esters induce desensitization of adenylate cyclase in rat tumour Leydig cells by different mechanisms. ...

The rapid activation of the mechanistic target of rapamycin complex-1 (mTORC1) by growth factors is increased by extracellular amino acids through yet-undefined mechanisms of amino acid transfer into endolysosomes. Because the endocytic process of macropinocytosis concentrates extracellular solutes into endolysosomes and is increased in cells stimulated by growth factors or tumor-promoting phorbol esters, we analyzed its role in amino acid-dependent activation of mTORC1. Here, we show that growth factor-dependent activation of mTORC1 by amino acids, but not glucose, requires macropinocytosis. In murine bone marrow-derived macrophages and murine embryonic fibroblasts stimulated with their cognate growth factors or with phorbol myristate acetate, activation of mTORC1 required an Akt-independent vesicular pathway of amino acid delivery into endolysosomes, mediated by the actin cytoskeleton. Macropinocytosis delivered small, fluorescent fluid-phase solutes into endolysosomes sufficiently fast to ...

Author: Rauscher, Andreas et al.; Genre: Journal Article; Published in Print: 2000-09-07; Keywords: Vimentin; Gene expression; Electroporation; Stress; 12-O-Tetradecanoylphorbol-13-acetate; Plasmacytoma; MPC-11 cell; Title: Similar effects of electroporational stress and treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate on vimentin expression in mouse plasmacytoma cells

In this study, short-term phorbol ester exposure was found to prevent acute AA + Fe (also AA alone or iron alone) oxidative stress and toxicity in the CYP2E1-expressing E47 cells. 4-α-TPA, a TPA-derived biologically inactive molecule unable to activate PKC, was not protective. Accordingly, the mechanism by which TPA exerts its protection seems to be related to its ability to activate certain PKC isoform(s) (Nishizuka, 1984) rather than via a direct effect as an antioxidant molecule. The protective effect of TPA on CYP2E1-mediated AA + Fe toxicity in E47 cells was dose- and time-dependent and occurred simultaneously with an increased translocation of phosphorylated PKC to membranes.. Inhibitors of PKC can interact with the ATP/substrate-binding sites or with regulatory sites of the enzyme. TPA-stimulated PKC isoform(s) involved in the protective action of this phorbol ester on CYP2E1-dependent AA + Fe toxicity in E47 cells were sensitive to the PKC inhibitors Ro 31-8425 and staurosporine, which ...

T cell activation via the T cell receptor (T3-Ti complex) by OKT3 results in modulation of the T3-Ti complex, but does not affect T4, T8, or T11 antigen expression. To study the effect of other T cell activators on these T cell membrane antigens, the authors incubated mononuclear cells for 0-3 days with lectins or pharmacologic agents and stained with monoclonal antibodies to their antigens. The median fluorescence intensity (MFI) was measured with a fluorescence activated cell sorter. Activation of PBL with Con A, PHA, calcium ionophore A23187, or with dbcAMP, isoproterenol, or theophyllin had minimal effects on the MFI of T3, T4, T8, or T11. Phorbol myristate acetate (PMA), a protein kinase C activator which stimulates PBL though an alternate pathway, caused a 90-100% reduction of T3 and T4 MFI, a 25% reduction in T8 MFI, and a 400% increase in T11 MFI after 2 days. Addition of A23187 slightly increased these effects. PMA induced a 2-3-fold increase in cell diameter concomitant with the ...

I have started measuring in-situ Protein Kinase C activity in permeabilized cells grown in 96-well plates, based on a few references from the literature such as: Heasley L.E., J.Biol.Chem., 1989, 264, 8646. I always get high activity after stimulation with phorbol esters and very low activity after treatment with PKC inhibitors. The problem is that the activity in untreated cells is often almost as high as in stimulated cells. Any advice or protocol would be welcome. Thanks Bernard medbpl at emory.edu ...

Immunological evidence for two physiological forms of protein kinase C.: Our recently described purification scheme for rat brain protein kinase C yields an enz

Liponate® 2-DH is a very light dry diester that has a silky silicone-type feel, spreads rapidly to a lubricious non-tacky film and has... ...

TY - JOUR. T1 - Decrease in cytosolic calcium/phospholipid-dependent protein kinase activity following phorbol ester treatment of EL4 thymoma cells. AU - Kraft, A. S.. AU - Anderson, W. B.. AU - Cooper, H. L.. AU - Sando, J. J.. N1 - Copyright: Copyright 2004 Elsevier B.V., All rights reserved.. PY - 1982. Y1 - 1982. UR - http://www.scopus.com/inward/record.url?scp=0020356002&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0020356002&partnerID=8YFLogxK. M3 - Article. C2 - 7142138. AN - SCOPUS:0020356002. VL - 257. SP - 13193. EP - 13196. JO - Journal of Biological Chemistry. JF - Journal of Biological Chemistry. SN - 0021-9258. IS - 22. ER - ...

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The discovery that phorbol ester tumor promoters inhibit metabolic cooperation (MC) between several cell types in vitro fostered the idea that the effect might be associated with tumor promoters in general. The authors are testing the hypothesis by assessing the effects of tumor promoters, selected metabolites, and other chemicals on MC between cocultivated mutant (HGPRT-) and wild-type (HGPRT+) V79 cells in reconstructed selection experiments. Correlation studies show that many structurally-diverse promoters inhibit MC in a dose-related fashion. More recent studies show that ethanol, a surface-active solvent with cocarcinogenic activity in humans, inhibits MC at high concentrations (10-20 mg/ml). Phenol, a weak promoter of tumors on mouse skin, has no effect on MC at concentrations ranging from 50 to 400 micrograms/ml. Five metabolites of phenol block MC, but at least two others appear to be inactive as complete promoters for mouse skin ...

The report generally describes 2,2-difluoro-2-deoxycytidine-3,5-dibenzoate, examines its uses, production methods, patents. 2,2-Difluoro-2-deoxycytidine-3,5-dibenzoate

We demonstrate complementary differences in the behavior of B lymphoblastoid cells adhering to LFA-1 or its counter-receptor ICAM-1. The interaction of B lymphoblastoid cells with glass-supported planar bilayers bearing LFA-1 or ICAM-1 was observed by time-lapse video microscopy, and the distribution of adhesion receptors on cells interacting with the planar bilayers was studied by immunofluorescence microscopy. B lymphoblasts formed a large contact area and crawled rapidly (up to 25 microns/min) on planar bilayers bearing ICAM-1. In contrast, these cells attached to planar bilayers bearing LFA-1 through a fixed point about which the cells actively pivoted, using a single stalk-like projection. Phorbol ester-stimulated lymphoblasts, which adhere more strongly to ICAM-1-bearing substrates than unstimulated lymphoblasts, were still capable of locomotion on ICAM-1. Phorbol ester stimulation of B lymphoblasts on planar bilayers bearing LFA-1 promoted a rapid conversion from stalk attachment to symmetrical

Some of the more common and easily measured volatile compounds of wine were determined for three different fermentation temperatures. Maximum, minimum, and average are given for over 30 fermentations at each temperature [(50, 70, 91°F) or (10, 21, 33°C)] for two years. Volatile acidity and acetic acid were compared and found to be equivalent for all practical purposes. Total volatile esters increase at the middle temperatures, as do acetaldehyde, isoamyl, and active amyl alcohols. Acetic acid decreases in the middle fermentation temperature range. Isobutanol does not vary greatly with fermentation temperature. Ethanol decreases with increasing fermentation temperature. Both levo and meso 2,3-butanediol increase with increasing temperature of fermentation. Acetoin also increases greatly at the higher fermentation temperatures.. ...

TY - JOUR. T1 - Properties of membrane-inserted Protein Kinase C. AU - Bazzi, Mohammad D.. AU - Nelsestuen, Gary L.. PY - 1988. Y1 - 1988. N2 - Protein kinase C (PKC) interacted with phospholipid vesicles in a calcium-dependent manner and produced two forms of membrane-associated PKC: a reversibly bound form and a membrane-inserted form. The two forms of PKC were isolated and compared with respect to enzyme stability, cofactor requirements, and phorbol ester binding ability. Membrane-inserted PKC was stable for several weeks in the presence of calcium chelators and could be rechromatographed on gel filtration columns in the presence of EGTA without dissociation of the enzyme from the membrane. The activity of membrane-inserted PKC was not significantly influenced by Ca2+, phospholipids, and/or PDBu. Partial dissociation of this PKC from phospholipid was achieved with Triton X-100, followed by dialysis to remove the detergent. The resulting free PKC appeared indistinguishable from original free ...

Effects of chlorella on activities of protein tyrosine phosphatases, matrix metalloproteinases, caspases, cytokine release, B and T cell proliferations, and phorbol ester receptor binding. Hot water extract of C. vulgaris triggered the death of human liver cancer cells by increasing p53, Bax, and caspase-3. Ever try supplements that dont seem to work? They should guide further investigation but shouldnt be interpreted as supportive of the anticancer effects until more research is done. Ohtake, T., Negishi, K., Okamoto, K., Oka, M., Maesato, K., Moriya, H., and Kobayashi, S. Manganese-induced Parkinsonism in a patient undergoing maintenance hemodialysis. Although it was beneficial for decreasing heart disease risk factors and fat cell size, there was no significant evidence that it could help with weight loss [13]. We are dedicated to providing the most scientifically valid, unbiased, and comprehensive information on any given topic. Chlorella may also cause photosensitivity, increasing the ...

TY - JOUR. T1 - Synthesis and protein kinase C binding activity of benzolactam-V7. AU - Ma, Dawei. AU - Wang, Guoqiang. AU - Wang, Shaomeng. AU - Kozikowski, Alan P.. AU - Lewin, Nancy E.. AU - Blumberg, Peter M.. PY - 1999/5/17. Y1 - 1999/5/17. N2 - Benzolactam-V7 (3a), a simplified analogues of (-)-indolactam-V with twist-form conformation, was synthesized and evaluated as a new protein kinase C modulator. Both 3a and its-7-substituted analogue 3c showed weak binding activity to displace PDBU binding from recombinant PKCα.. AB - Benzolactam-V7 (3a), a simplified analogues of (-)-indolactam-V with twist-form conformation, was synthesized and evaluated as a new protein kinase C modulator. Both 3a and its-7-substituted analogue 3c showed weak binding activity to displace PDBU binding from recombinant PKCα.. UR - http://www.scopus.com/inward/record.url?scp=0033577690&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0033577690&partnerID=8YFLogxK. U2 - ...

Thoracic aortas (TA) were excised from 6-week old SHR and WKY. 2mm rings were mounted isometrically at optimum preload. Spontaneous rhythmical activity developed in TA from SHR and had a frequency of 3-4/min with varying periods of quiescence between bursts of activity. The spontaneous activity often produced an increase in tension development which was associated with increased frequency of oscillations. Verapamil (10/sup -7/ M) or Ca/sup + +/-free solution added during the contractile phase resulted in an immediate loss of tension and spontaneous activity. Addition of ouabain (10/sup -4/ M) during the contractile phase of spontaneous activity, increased the frequency of oscillations which appeared to fuse into a tetanus. Spontaneous rhythmical activity was infrequently observed in TA from WKY. However, addition of phorbol 12-myristate-13 acetate (TPA), frequently induced spontaneous rhythmic oscillations associated with tension development in TA from WKY. TPA contracted the SHR TA and ...

From Nature Biotechnology Press Release.) Current estimates predict that genome sequencing efforts may reveal between 3,000 and 10,000 new targets for drugs. In the December issue of Nature Biotechnology, Paul Negulescu and colleagues describe a rapid and sensitive assay for identifying such gene targets in live human cells. In their approach, a reporter gene encoding β-lactamase was introduced into human T lymphocytes in an attempt to tag virtually every gene in the genome. When these cells were exposed to phorbol ester-a chemical that primes T lymphocytes for action against foreign agents-gene sequencing revealed 10 tagged genes that had previously never been linked with lymphocyte activation. By rapidly selecting and enriching cells containing these genes using a method called flow cytometry, it should be possible to develop screens for rapidly assessing drug candidates.. Negulescus team were able to assess the T lymphocyte genes that responded to phorbol ester by measuring expression ...

PKC gamma, 0.1 ml. PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes

Effect of PMA treatment on the expression of cyclins and cdks in IEC-18 cells. Cells were exposed to 100 nM PMA for the indicated times (U, untreated) and subje

Effects of pentoxifylline and protein kinase C inhibitor on phorbol ester-induced intercellular adhesion molecule-1 expression in brain microvascular endothelial cells.

The activity of calcium-, phospholipid-dependent protein kinase (PKc) was measured in (a) total extracts, (b) crude membrane, and (c) cytosolic fractions of chick embryo myogenic cells differentiating in culture. Total PKc activity slowly declines during the course of terminal myogenesis in contrast to the activity of cAMP-dependent protein kinase, which was also measured in the same cells. Myogenic cells at day 1 of culture possess high particulate and low soluble PKc activity. A dramatic decline of particulate PKc activity occurs during myogenic cell differentiation and is accompanied, through day 4, by a striking rise of the soluble activity. The difference in the subcellular distribution of PKc between replicating myoblasts and myotubes is confirmed by phosphorylation studies conducted in intact cells. These studies demonstrate that four polypeptides whose phosphorylation is stimulated by the tumor promoter 12-O-tetradecanoyl phorbol 13-acetate in myotubes, are spontaneously phosphorylated ...

TY - JOUR. T1 - Asialoglycoprotein receptor phosphorylation and receptor-mediated endocytosis in hepatoma cells. Effect of phorbol esters. AU - Fallon, R. J.. AU - Schwartz, A. L.. PY - 1988/1/1. Y1 - 1988/1/1. N2 - The asialoglycoprotein (ASGP) receptor on Hep G2 cells undergoes constitutive recycling and ligand endocytosis in the presence of phorbol dibutyrate, at a 50% reduced rate relative to control cells (Fallon, R. J., and Schwartz, A. L. (1986) J. Biol. Chem. 261, 15081-15089). The relevance of receptor phosphorylation to these events was investigated by selective immunoprecipitation of surface receptors with polyclonal anti-human ASGP antiserum and pulse-chase labeling with [32P]orthophosphate to identify subcellular locations of initial receptor phosphorylation events as well as the eventual fate of phosphorylated receptor during recycling. The surface immunoprecipitation method recovers greater than 95% of surface ASGP receptors and only 5% or less of intracellular ...

sn-1,2-Didecanoylglycerol, a synthetic lipid second messenger and model diacylglycerol, was evaluated as a complete skin tumor promoter in CD-1 mice. In addition, sn-1,2-dioctanoylglycerol, sn-1,2-didecanoylglycerol, the second stage tumor promoter mezerein, and the complete tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) were examined for their ability to stimulate epidermal protein kinase C activity in vitro. All four compounds stimulated epidermal protein kinase C activity utilizing lysine-rich histone as the phosphate acceptor substrate. sn-1,2-Dioctanoylglycerol and sn-1,2-didecanoylglycerol stimulated epidermal protein kinase C activity to a maximum velocity similar to that obtained when the enzyme was stimulated with TPA; however, about 1000 times greater concentration of the sn-1,2-diacylglycerols was required. sn-1,2-Didecanoylglycerol was evaluated as a complete skin tumor promoter in CD-1 mice utilizing a dosing regimen demonstrated to produce epidermal hyperplasia. Mice ...

The protein kinase C (PKC) activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA) activated cell death in androgen-sensitive LNCaP cells but not in androgen-independent DU-145 or PC-3 cells, whose growth was significantly decreased by PKC inhibitors staurosporine and H7. All cell lines had similar le …

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Proteins kinase C (PKC) isozymes have been implicated as regulators of signaling pathways that promote proliferation survival metastasis and drug resistance in malignancy cells [1 2 Elevated levels of PKC expression or activity have been noted in human malignancies such as gliomas [3] breast tumors [4] and metastatic gastric carcinoma [5]. inhibitor of PKC that operates through a novel mechanism binding to a Ca2+-induced hydrophobic site around the PKC regulatory domain name and preventing activation by diacylglycerol (DAG) and phorbol esters [8 9 The inhibitory activity of cal-C is usually strictly dependent on photoexcitation which causes irreversible site-specific oxidative modification of PKC [10 11 This has raised the prospect that cal-C might be a useful agent for photodynamic malignancy therapy [12]. Thus far the evaluation of cal-C has been limited to preclinical studies. The results have established that this inhibitor can Mouse monoclonal to CD152(PE). induce apoptosis in a broad ...

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No dermal reactions were observed following a single semi-occlusive application of DEGDB to intact rabbit skin for four hours. Neat test material (0.1 ml) was instilled into one eye of six healthy adult rabbits, and the effects observed for three days (72h). Transient hyperaemia of blood vessels only was observed in all animals. These reactions had resolved in all instances by one or two days after instillation. No corneal damage or iridial inflammation was observed. Similar findings were seen in a much earlier (non-GLP) study and the same conclusion was made. As a single instillation of DEGDB into the rabbit eye did not elicit a positive response according to the established test criteria, it is therefore considered as non-irritant. ...

How is TPA and 1-oleoyl-2-acetyl-glycerol abbreviated? OAG stands for TPA and 1-oleoyl-2-acetyl-glycerol. OAG is defined as TPA and 1-oleoyl-2-acetyl-glycerol very rarely.

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