In order to understand the mechanism of molecular interactions at the active site of Tryparedoxin Peroxidase (Try P), homology modeling and docking studies were performed. We generated a Three-Dimensional (3D) model of target protein based on the Crystal structure of Leishmania Major Try PI (PDB ID: 3TUE) using modeler software. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (Try P). Inhibition of the Tryparedoxin peroxidase interaction has become a new therapeutic strategy in treating leishmaniasis. Docking analysis was carried out to study the effects of methotrexate on Tryparedoxin Peroxidase (TryP). Tryparedoxin peroxidase of Trypanosomatidae family functions as antioxidant through their peroxidase and peroxynitrite reductase activities. The theoretical docking study, conducted on a sample previously reported for anti-cancer properties of Methotrexate at the binding site of 3D models of Tryparedoxin Peroxidase of Leishmania braziliensis (L. ...
TY - JOUR. T1 - Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells. T2 - Possible involvement of peroxidase isozymes in aluminum ion stress. AU - Ezaki, Bunichi. AU - Tsugita, Shinobu. AU - Matsumoto, Hideaki. PY - 1996/1. Y1 - 1996/1. N2 - To clarify the mechanism of aluminum (Al) toxicity and Al tolerance, we isolated a new clone (pAL201) from a tobacco cDNA library. Northern blot hybridization analysis indicated that the expression of pAL201 is induced by Al treatment and phosphate (Pi) starvation. The complete cDNA sequence suggested that this clone encodes a moderately anionic peroxidase (EC 1.11.1.7). Analysis by isoelectric focussing indicated that a moderately anionic peroxidase (approximately pI 6.7) and two cationic peroxidases (pI 9.2 and 9.7) in the soluble fraction are activated by Al treatment and Pi starvation, while two moderately anionic isozymes are repressed by these stresses. We suppose that Al ion stress can control the activity ...
Heme peroxidases are widely distributed in biological systems and are involved in a wide range of processes essential for life. This book provides a comprehensive single source of information on the various aspects of heme peroxidase structure, function and mechanism of action. Chapters written and edited by worldwide experts span a range of heme peroxidases from plants, yeast, bacteria and mammals. Discussed functions of peroxidases range from cell wall synthesis, synthesis of prostaglandins, role in drug suppression of tuberculosis, and antibacterial activity. Included is a discussion of peroxidases that also act as catalases and oxygenases. Heme Peroxidases serves as an essential text for those working in industry and academia in biochemistry and metallobiology.
Minute Peroxidase Suppressor is designed to inhibit endogenous peroxidase activity commonly encountered in immunohistochemistry (IHC) procedures. Inhibiting endogenous peroxidase activity is essential for avoiding false positive and reduce the background of IHC. Many chemical agents such as H2O2 and sodium aside have been used for this purpose but the inhibition is usually incomplete. Minute TM HRP Suppressor is a mixture of several potent HRP inhibitors and the result is a complete inhibitionof endogenous peroxidase activity. The suppressor can be used in regular IHC as well as HRP-mediated super sensitive procedures such as tyramide signal amplification (TSA). Major Features: Very stable at RT, ready to use and irreversible inhibition of endogenous peroxidase activity. Significantly reduce the background of sensitive assays using HRP. 1. After cell or tissue samples are prepared on slide, perform antigen retrieval step if necessary. 2. Block non-specific sites in the samples with normal serum ...
Infinite Enzymes Manganese Peroxidase Enzyme (MnP) is a recombinant peroxidase from Phanerochaete chrysosporium produced in corn seed. Request Quote now!
Alterations in Soluble Class III Peroxidases of Maize Shoots by Flooding Stress. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Thiol peroxidases are critical enzymes in the redox control of cellular processes that function by reducing low levels of hydroperoxides and regulating redox signaling. These proteins were also shown to regulate genome stability, but how their dysfunction affects the actual mutations in the genome is not known. Saccharomyces cerevisiae has eight thiol peroxidases of glutathione peroxidase and peroxiredoxin families, and the mutant lacking all these genes (Δ8) is viable. In this study, we employed two independent Δ8 isolates to analyze the genome-wide mutation spectrum that results from deficiency in these enzymes. Deletion of these genes was accompanied by a dramatic increase in point mutations, many of which clustered in close proximity and scattered throughout the genome, suggesting strong mutational bias. We further subjected multiple lines of wild-type and Δ8 cells to long-term mutation accumulation, followed by genome sequencing and phenotypic characterization. Δ8 lines showed a ...
White-rot fungi (WRF) are capable of degrading complex organic compounds such as lignin, and the enzymes that enable these processes can be used for the detoxification of recalcitrant organopollutants. The aim of this study is to evaluate a system based on the use of an in vitro ligninolytic enzyme for the detoxification of recalcitrant dye pollutants. The dyes selected for investigation were the anionic and cationic commercial azo dyes, basic blue 41 (BB41), acid black 1 (AB1), and reactive black 5 (RB5). A supernatant, cell-free culture of WRF with manganese peroxidase activity was used to investigate its degradative capacity under various conditions, and concentrations of cofactors, H2O2 and Mn2+. The assays were carried out using a 22 experimental designs whose variables were concentration of Mn2+ (33 and 1,000 μM) and semicontinuous dosage of the H2O2 (0.02 and 0.10 μmol) added at a frequency of 0.2 min−1. The response variables analyzed were the efficiency and the initial rate of the ...
산화적 스트레스(Oxidative Stress)는 Reactive Oxygen Species (ROS)와 antioxidant (항산화제)간의 불균형에 의해 발생되며, ROS의 과도한 축적은 DNA, 단백질 및 지질 세포막의 손상과 같은 세포적 손상을 초래합니다.. 산화적 스트레스의 조건에서 발생되는 Hydrogen Peroxide (H2O2)와 같은 과산화물(peroxide)은 ROS의 대표적인 산물 중 하나로 진핵세포에서는 유독하며, 높은 농도에서는 DNA, 지질 단백질의 산화를 일으켜 돌연변이 유발이나 세포사멸을 일으키기도 합니다.. peroxide에 의한 세포적 손상은 노화, 천식, 관절염, 당뇨병, 심혈관 질환, 신경성 퇴행성 질환 등 여러 질환의 발병과도 관계가 있습니다. EZ-Hydrogen Peroxide/Peroxidase assay kit는 Oxi-Probe를 사용하여 Hydrogen Peroxide (H2O2) 또는 Peroxidase 활성을 측정할 수 있는 제품으로 간단한 실험 방법과 민감도를 가지는 제품입니다. 본 Kit는 ...
Title: Recents Patents in the Use of Peroxidases. VOLUME: 3 ISSUE: 2. Author(s):Berenize Alvarado and Eduardo Torres. Affiliation:Benemerita Universidad Autonoma de Puebla, Centro de Quimica-ICUAP. Ciudad Universitaria, Puebla, 72570. Mexico.. Keywords:Biosensor, enzymatic oxidation, peroxidases, review. Abstract: Peroxidases are hemoenzymes with a wide range of applications, from fine chemical synthesis to environmental biocatalysis. These outstanding biocatalysts are able to catalyze reactions such as heteroatom oxidation (Nand S-oxidation), epoxidation, hydroxylation, and the oxidation of alcohols and indole, often giving high yields and enantiomeric excess values. This makes these biocatalysts very useful for application to several biotechnological processes. In this paper, recent advances and patents surrounding the use of peroxidases are reviewed, covering different aspects related to the applications of peroxidases and the modifications carried out to improve their functionality as ...
casSAR Dugability of Q73WB6 | MAP_2744c | Catalase-related peroxidase - Also known as CRPE_MYCPA, MAP_2744c. Has an organic peroxide-dependent peroxidase activity. Exhibits strong peroxidase activity using organic hydroperoxides as cosubstrates, weak peroxidase activity using hydrogen peroxide and negligible catalase activity. May have a role in elimination of reactive oxygen species, in particular by deactivating hydroperoxides. Monomer.
There is another endogenous peroxidase blocking method the blocks peroxidases and pseudoperoxidases. It can be used for minimally fixed frozen sections and in your case, paraffin sections. The is very thorough and gentle. It is called Glucose Oxidase blocking method. We love it for frozens as it does not chew sections off the slide. We have not tried it on paraffin but I have it by private communication it will work with paraffin sections/NBF fixed. I have seen it reported as 30 min in the working solution but original publication used it for 60 min. I will be happy to attach the method to you, not via Histonet. At 09:21 PM 6/23/2004 -0400, you wrote: >Todd, > Youre right. Sorry I havent responded to everyones suggestions >on my posting on blocking endogenous peroxidase in paraffin >sections. I think those who suggested that biotin might be the >problem have a point, however when I ran control sections with >just buffer, blocking (10% goat serum), and DAB, I still got >brown, even though ...
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Hamster mast cells have been found to give strong peroxidatic reactions at pH 5, 7.5 and 10 when sections of skeletal muscle are incubated for 2.5 h in the dark at room temperature on semipermeable...
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Enzyme Explorer - Peroxidase Enzymes products. Sigma-Aldrichs peroxidase product is recognized around the world as the industry standard for diagnostic manufacturing and laboratory scale research applications.
DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling.
Vector|sup|®|/sup| NovaRED™ and ImmPACT™ NovaRED™ HRP substrates produce red reaction products. Unlike AEC, sections stained with either substrate should be dehydrated, cleared, and permanently mounted.
1. Thaw plates before opening bags to prevent condensation in the wells. 2. Add 200ul Blocking Buffer to each well and incubate 1 hour @ room temperature (RT). 3. Wash 3x w/ PBST. Aspirate and blot dry. 4. Prepare standards and samples in PBS. 5. Add 100ul of dilutions to wells. 6. Incubate plate 2-3h @ RT. 7. Incubate o/n @ 4C. 8. Wash 3x w/ PBST. Aspirate and blot dry. 9. Dilute biotinylated mAB211 in PBST + 0.5% Bovine Serum Albumin (BSA). 10. Add 100ul diluted Ab to each well. Incubate 2h @ RT. 11. Wash 3x w/ PBST. Aspirate and blot dry. 12. Prepare HRP-Streptavidin...... 13. Add 100ul diluted HRP-Streptavidin to each well. Incubate 2h @ RT. 14. Wash 3x w/ PBST. Aspirate and blot dry. 15. Prepare Peroxidase Substrate immediately before use. 16. Add 100ul Peroxidase Substrate to each well and incubate 10min in the dark @ RT. 17. Add 100ul Stopper Solution to wells and read plate at 490nm. ...
peroxidase, putative; FUNCTIONS IN: electron carrier activity, peroxidase activity, heme binding; INVOLVED IN: response to oxidative stress; LOCATED IN: endomembrane system; EXPRESSED IN: 10 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant peroxidase (InterPro:IPR000823), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: peroxidase, putative (TAIR:AT5G58400.1); Has 2891 Blast hits to 2875 proteins in 212 species: Archae - 0; Bacteria - 4; Metazoa - 2; Fungi - 66; Plants - 2777; Viruses - 0; Other Eukaryotes - 42 (source: NCBI BLink ...
Streptococcus pneumoniae is a facultative anaerobic pathogen. Although it maintains fermentative metabolism, during aerobic growth pneumococci produce high levels of H2O2, which can have adverse effects on cell viability and DNA, and influence pneumococcal interaction with its host. The pneumococcus is unusual in its dealing with toxic reactive oxygen species in that it neither has catalase nor the global regulators of peroxide stress resistance. Previously, we identified pneumococcal thiol peroxidase (TpxD) as the key enzyme for enzymatic removal of H2O2, and showed that TpxD synthesis is up-regulated upon exposure to H2O2. This study aimed to reveal the mechanism controlling TpxD expression under H2O2 stress. We hypothesize that H2O2 activates a transcription factor which in turn up-regulates tpxD expression. Microarray analysis revealed a pneumococcal global transcriptional response to H2O2. Mutation of tpxD abolished H2O2-mediated response to high H2O2 levels, signifying the need for an active TpxD
Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT), Peroxidase Conjugated Affinity Purified anti-Sheep IgG F(ab )2 [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT)IGHG1
cis-regulatory elements of the peroxidase gene in Arabidopsis thaliana involved in root-specific expression and responsiveness to high-salt stress.: The pattern
The Determination of Peroxidase in Amniotic Fluid.: Analyzing total peroxidase activity in amniotic fluid is extremely simple, requiring only 1 1/2 minutes of i
Within biological systems iron is a transition metal that allows access to the benefits of molecular oxygen as an oxidant. However, with these benefits come grave consequences if the reactions are not strictly controlled. The most prominent strategy of control and specialization is the protein environment that surrounds iron. Within iron containing proteins, specifically heme proteins, there are four basic levels of structure that impact the irons function: cofactor structure, protein-supplied ligands, non-ligand active site environment, and protein features that are distant from the active site. This last level remains poorly understood due to a lack of good models to pursue such studies. Catalase-peroxidases are unique heme proteins because they catalyze peroxide decomposition by two separate mechanisms, catalase and peroxidase, using the same active site. However, were it not for three structural features distant from the active site, catalase-peroxidases would be practically superimposable ...
1NHQ: Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
Purified Native R. communis Lectin Toxin RCA60, Peroxidase labeled from Creative Biomart. Native R. communis Lectin Toxin RCA60, Peroxidase labeled can be used for research.
1NHP: Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, and an unusual environment of Arg 303.
is this on human tissues? abizar www.innogenex.com 1-877-igx-info -----Original Message----- From: [email protected] [mailto:[email protected]] Sent: Wednesday, May 03, 2000 3:22 PM To: [email protected] Subject: controls for rabbit polyclonals Plea for help, I am unable to get clean control slides for any rabbit polyclonal antibodies on either paraffin or frozen sections. There is always background (often very dark) on the collagen, smooth muscle (especially prostate) etc. This happens with commercial antibodies (B2microglobulin or vonWillebrand), in house Abs, as well as with rabbit IgG. I use casein or superblock. Avidin-Biotin block and exhaust endogenous peroxidase. I use the ABC kit with DAB. There is no problem if I omit the primary antibody. Can anybody offer any insights? Thank you, Bonnie ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Sodium atom in PDB 2b2r: Crystal Structure Of An Oxoferryl Species of Catalase- Peroxidase Katg At PH5.6
peroxidase: Any of a group of enzymes that occur especially in plant cells and catalyze the oxidation of a substance by a peroxide.
anti-Glutathione Peroxidase 7, Polyclonal, Novus Biologicals 0.1mL; Unlabeled Life Sciences:Antibodies:Primary Antibodies:Immunoprecipitation (IP)
Histofine Simple Stain MAX Peroxidase (Human Tissue, Mouse primary): 414131F by As One International, Inc. at Labscoop.com - Read reviews, citations, datasheets, protocols & more.
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Click on a taxon to see the following taxons, if there are any.. By placing the mouse over a node a tooltip will appear. This tootip contains the taxonomic path from "Cellular organisms" to the node and, in the case of a species, the number of peroxidases in the orthogroup, the total number of peroxidases for that species in the orthogroups class and where possible the names of those peroxidases. The common ancestor for the orthogroup is written in bold in the tooltip. Return to orthogroup ...
The orthogroup DiHCcP001 is composed of 84 sequences in 68 species. Click here to see a graphical representation of the species in this orthogroup.. ...
C.E.T. Chews For Dogs Lg 30 Ct. 30 count bag of beefhide strips that average 2 x 6 inches. Treated with the C.E.T. Dual-Enzyme System which enhances and augments a naturally-occurring oral defense mechanism, the Salivary Peroxidase System.
Dear Experts, I would like to label an antibody with peroxidase by the periodate method. Which HRP grade / isoforms / purity should I use? Is there a practical advantage (or even disadvantage) of stabilized peroxidase, where the amino groups are chemically protected and therefore oligomerization of the HRP would be prevented? The AB will be used in westerns and Elisa. Many thanks! Wo ...
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A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a transit peptide to chloroplasts at its ammo-terminus and a putative membrane-spanning region at its carboxy-terminus.. ...
The ionic strength dependence of the reduction of cytochrome c peroxidase compound I by horse cytochrome c has been studied, using stopped-flow technigue, in pH 7.5, potassium phosphate/nitrate buffer. The temperature was set at 25 ± 1° C. The wavelength was monitored at 4 32 nm, an isobestic point for ferri-/ferrocytochrome c, so only the absorbance change of the reduction of cytochrome c peroxidase compound I to compound II can be observed. The observed rate constant, kobs, as a function of the concentration of ferrocytochrome c shows a non-linear increase with increasing ionic strength. A two-parameter eguation is needed to fit the data at low ionic strength, 20 mM to 40 mM, while a three-parameter equation is needed at high ionic strength, 65 mM and above. The maximum rates of these reductions also show two different types of ionic strength dependence. At 20 mM to 40 mM ionic strength, the maximum rate of reduction decreases slightly, within experimental error, with increasing ionic ...
We previously demonstrated that manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium was very susceptible to thermal inactivation due to the loss of calcium from the enzyme [Sutherland & Aust (1996) Arch. Biochem. Biophys. 332, 128-134]. The structural changes that occur during thermal inactivation and the release of calcium from manganese peroxidase have now been characterized. Thermal inactivation caused distinct alterations in the heme environment and slight changes in the overall protein structure, both of which were reversed upon reactivation of the enzyme with calcium. The absorption spectrum of inactivated manganese peroxidase was similar to that of low-spin ferric heme proteins, indicating that a sixth ligand had bound to the distal side of the heme iron. Consistent with disruption of the distal heme environment, thermally inactivated manganese peroxidase did not react with hydrogen peroxide to form compound I. The inactive enzyme exhibited a pH-dependent absorption
Species of the genus Pleurotus are among the most efficient natural species in lignin degradation belonging to the subclass of ligninolytic organisms that produce laccase (Lac), Mn-dependent peroxidase (MnP), versatile peroxidase (VP), and the H2O2-generating enzyme aryl-alcohol oxidase, but not lignin peroxidases. Production of Lac and oxidation of 2,6-dimethoxyphenol (DMP) in the presence and absence of Mn2+ were detected both in submerged fermentation (SF) of dry ground mandarine peels and in solid-state fermentation (SSF) of grapevine sawdust in all investigated Pleurotus species and strains. Evidence of cultivation methods having a distinct influence on the level of enzyme activities has been demonstrated. Most of the species and strains had higher Lac activity under SSF conditions than under SF conditions. DMP oxidation in the presence and absence of Mn2+ was detected in all investigated species and strains, but was lower under SF conditions than under SSF conditions for most of ...them. ...
Additional file 19: of Genome-wide and evolutionary analysis of the class III peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses
Author(s): Smith, Martyn T.; Yager, J; Steinmetz, K; Eastmond, D | Abstract: The metabolism of two of benzenes phenolic metabolites, phenol and hydroquinone, by peroxidase enzymes has been studied in detail. Studies employing horseradish peroxidase and human myeloperoxidase have shown that in the presence of hydrogen peroxide phenol is converted to 4,4-diphenoquinone and other covalent binding metabolites, whereas hydroquinone is converted solely to 1,4-benzoquinone. Surprisingly, phenol stimulates the latter conversion rather than inhibiting it, an effect that may play a role in the in vivo myelotoxicity of benzene. Indeed, repeated coadministration of phenol and hydroquinone to B6C3F1 mice results in a dramatic and significant decrease in bone marrow cellularity similar to that observed following benzene exposure. A mechanism of benzene-induced myelotoxicity is therefore proposed in which the accumulation and interaction of phenol and hydroquinone in the bone marrow and the peroxidase-dependent
Poole, L.B., and Ellis, H.R. (1996) Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 1. Purification and enzymatic activities of overexpressed AhpF and AhpC proteins. Biochemistry 35, 56-64. PDF of article Poole, L.B. (1996) Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 2. Cystine disulfides involved in catalysis of peroxide reduction. Biochemistry 35, 65-75. PDF of article Poole, L.B., Chae, H.Z., Flores, B.M., Reed, S.L., Rhee, S.G., and Torian, B.E. (1997) Peroxidase activity of a TSA-like antioxidant protein from a pathogenic amoeba. Free Radical Biol. Med. 23, 955-959. PDF of article Li Calzi, M., and Poole, L.B. (1997) Requirement for the two AhpF cystine disulfide centers in catalysis of peroxide reduction by alkyl hydroperoxide reductase. Biochemistry 36, 13357-13364. PDF of article Ellis, H. R., and Poole, L.B. (1997) Roles for the two cysteine residues of AhpC in catalysis of peroxide reduction by alkyl hydroperoxide reductase ...
thyroid peroxidase - MedHelps thyroid peroxidase Center for Information, Symptoms, Resources, Treatments and Tools for thyroid peroxidase. Find thyroid peroxidase information, treatments for thyroid peroxidase and thyroid peroxidase symptoms.
Lactoperoxidase is a peroxidase enzyme secreted from mammary, salivary, and other mucosal glands that functions as a natural antibacterial agent. Lactoperoxidase is a member of the heme peroxidase family of enzymes. In humans, lactoperoxidase is encoded by the LPO gene. Lactoperoxidase catalyzes the oxidation of a number of inorganic and organic substrates by hydrogen peroxide. These substrates include bromide and iodide and therefore lactoperoxidase can be categorised as a haloperoxidase. Another important substrate is thiocyanate. The oxidized products produced through the action of this enzyme have potent bactericidal activities. Lactoperoxidase together with its inorganic ion substrates, hydrogen peroxide, and oxidized products is known as the lactoperoxidase system. The lactoperoxidase system plays an important role in the innate immune system by killing bacteria in milk and mucosal (linings of mostly endodermal origin, covered in epithelium, which are involved in absorption and secretion) ...
en] The cloning and sequencing of the rbpa gene coding for a versatile peroxidase from a novel Bjerkandera strain is hereby reported. The 1777 bp isolated fragment contained a 1698 bp peroxidase-encoding gene, interrupted by 11 introns. The 367 amino acid-deduced sequence includes a 27 amino acid-signal peptide. The molecular model, built via homology modelling with crystal structures of four fungal peroxidases, highlighted the amino acid residues putatively involved in manganese binding and aromatic substrate oxidation. The potential heme pocket residues (R44, F47, H48, E79, N85, H177, F194 and D239) include both distal and proximal histidines (H48 and H177). RBP possesses potential calcium-binding residues (D49, G67, D69, S71, S178, D195, T197, I200 and D202) and eight cysteine residues (C3, C15, C16, C35, C121, C250, C286, C316). In addition, RIBP includes residues involved in substrate oxidation: three acidic residues (E37, E41 and D183)-putatively involved in manganese binding and H83 and ...