TY - JOUR. T1 - Retinoic acid is a potential negative regulator for differentiation of human periodontal ligament cells. AU - Shibuya, Natsuko. AU - Nemoto, Eiji. AU - Kanaya, Sousuke. AU - Kunii, Ryotaro. AU - Shimauchi, Hidetoshi. PY - 2005/9/1. Y1 - 2005/9/1. N2 - Retinoic acid (RA) exerts a wide variety of effects on development, cellular differentiation and homeostasis in various tissues. However, little is known about the effects of RA on the differentiation of periodontal ligament cells. In this study, we investigated whether RA can affect the dexamethasone-induced differentiation of periodontal ligament cells. Human periodontal ligament cells were differentiated via culturing in the presence of dexamethasone, ascorbic acid, and β-glycerophosphate for mineralized nodule formation, as characterized by von Kossa staining. Continuous treatment with all-trans-RA inhibited mineralization in a dose-dependent manner, with complete inhibition over 1 μM RA. Other RA analogs, 9-cis-RA and ...
BACKGROUND AND OBJECTIVE: The antimicrobial peptide LL-37 is expressed in periodontal tissue, and variations in LL-37 levels have been associated with periodontal disease. The effects of LL-37 on periodontal ligament cell function have not been described before. Here, we assess anti-inflammatory properties of LL-37 and investigate the effects of LL-37 on cell differentiation, cell proliferation and apoptosis in human periodontal ligament cells. MATERIAL AND METHODS: Periodontal ligament cells were obtained from teeth extracted for orthodontic reasons. Cytokine (interleukin-6) and chemokine (monocyte chemoattractant protein-1) expression was determined by quantitative PCR, cell differentiation by alkaline phosphatase activity, cell proliferation by counting cells in a Bürker chamber, DNA synthesis by incorporation of radiolabeled thymidine and apoptosis by cell morphology and activated caspase 3 quantities. RESULTS: Treatment with 0.1 and 1 μm of LL-37 totally reversed ...
The oral cavity is a complex environment in which periodontal tissue is constantly stimulated by external microorganisms and mechanical forces. Proper mechanical force helps maintain periodontal tissue homeostasis, and improper inflammatory response can break the balance. Periodontal ligament (PDL) cells play crucial roles in responding to these challenges and maintaining the homeostasis of periodontal tissue. However, the mechanisms underlying PDL cell property changes induced by inflammatory and mechanical force microenvironments are still unclear. Recent studies have shown that exosomes function as a means of cell-cell and cell-matrix communication in biological processes. Human periodontal ligament stem cells (HPDLSCs) were tested by the CCK8 assay, EdU, alizarin red, and ALP staining to evaluate the functions of exosomes induced by a mechanical strain. MicroRNA sequencing was used to find the discrepancy miRNA in exosomes. In addition, real-time PCR, FISH, luciferase reporter assay, and western
Fibroblast growth factor 2 (FGF2) has been revealed to promote human periodontal ligament stem cell (PDLSC) proliferation. The abnormal proliferation of PDLSCs has also been associated with the pathogenesis of periodontitis. The long non‑coding RNA, metastasis‑associated lung adenocarcinoma transcript 1 (MALAT1), has been demonstrated to regulate FGF2 secretion. Therefore, MALAT1 may also be associated with periodontitis. The aim of the present study was to investigate the effect of MALAT1 overexpression on the proliferation of PDLSCs. In the current study, PDLSCs derived from healthy and periodontitis‑affected teeth were collected. MALAT1 and FGF2 mRNA expression in PDLSCs was detected using reverse transcription‑quantitative PCR. PDLSCs overexpressing MALAT1 were subsequently generated. PDLSC proliferation was analyzed using a Cell Counting kit‑8 assay. FGF2 protein expression was detected using western blot analysis. The results revealed that MALAT1 and FGF2 mRNA were significantly ...
Objective: This study aimed to investigate the roles of long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) in osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in periodontitis.Methods: Differentially expressed lncRNAs and mRNAs between periodontitis periodontal ligament tissues and healthy periodontal ligament tissues were selected out using R project. PDLSCs were identified using flow cytometry. Western blot was employed to detect pathway relative proteins. Besides, targeted relationships between lncRNA and miRNA, as well as miRNA and mRNA were verified by dual luciferase reporter gene assay. Osteogenic differentiation of PDLSCs was assessed by alkaline phosphatase (ALP) staining and Alizarin Red Staining (ARS). Markers for osteoblast (Runx2, Osterix, Osteocalcin, Colla1) were detected using western blot.Results: LncRNA MEG3 and IGF1 were both down-regulated, while miR-27a-3p was up-regulated in periodontitis samples compared with healthy samples. Overexpression
Cryopreservation has been extensively applied to the long-term storage of a diverse range of biological materials. However, no comprehensive study is currently available on the cryopreservation of periodontal ligament stem cell (PDLSC) sheets which have been suggested as excellent transplant materials for periodontal tissue regeneration. The aim of this study is to investigate the effect of cryopreservation on the structural integrity and functional viability of PDLSC sheets. PDLSC sheets prepared from extracted human molars were divided into two groups: the cryopreservation group (cPDLSC sheets) and the freshly prepared control group (fPDLSC sheets). The cPDLSC sheets were cryopreserved in a solution consisting of 90% fetal bovine serum and 10% dimethyl sulfoxide for 3 months. Cell viability and cell proliferation rates of PDLSCs in both groups were evaluated by cell viability assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, respectively. The multilineage
TY - JOUR. T1 - Osteoblastic differentiation of periodontal ligament stem cells on non-stoichiometric calcium phosphate and titanium surfaces. AU - Winning, L. AU - Robinson, L. AU - Boyd, A. R.. AU - El Karim, I A. AU - Lundy, F T. AU - Meenan, BJ. N1 - Compliant in UIR; evidence uploaded to Other files Reference text: 1 Olivares-Navarrete R, Hyzy SL, Park JH, Dunn GR, Haithcock DA, Wasilewski CE, Boyan BD, Schwartz Z. Mediation of osteogenic differentiation of human mesenchymal stem cells on titanium surfaces by a Wnt-integrin feedback loop. Biomaterials 2011;32:6399-6411. CrossRef , PubMed , CAS , Web of Science® Times Cited: 50 2 Rausch-fan X, Qu Z, Wieland M, Matejka M, Schedle A. Differentiation and cytokine synthesis of human alveolar osteoblasts compared to osteoblast-like cells (MG63) in response to titanium surfaces. Dent Mater 2008 ;24:102-110. Jan CrossRef , PubMed , CAS , Web of Science® Times Cited: 71 3 Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, ...
During the last decade it has become clear that periodontal ligament fibroblasts may contribute to the in vitro differentiation of osteoclasts. We surveyed the current findings regarding their osteoclastogenesis potential. Periodontal ligament fibroblasts have the capacity to select and attract osteoclast precursors and subsequently to retract and enable migration of osteoclast precursors to the bone surface. There, fusion of precursors takes place, giving rise to osteoclasts. The RANKL-RANK-osteoprotegerin (OPG) axis is considered crucial in this process. Periodontal ligament fibroblasts produce primarily OPG, an osteoclastogenesis-inhibitory molecule. However, they may be influenced in vivo by direct or indirect interactions with bacteria or by mechanical loading. Incubation of periodontal ligament fibroblasts with bacteria or bacterial components causes an increased expression of RANKL and other osteoclastogenesis-stimulating molecules, such as tumor necrosis factor-α and macrophage-colony ...
OBJECTIVE To compare the mechanotransduction caused by cyclic and static mechanical strains in human periodontal ligament fibroblasts (hPDLFs) cultured under identical conditions. MATERIALS AND METHODS hPDLFs, originating from the same donors, were exposed either to cyclic or to static tensile strain using specially designed devices and under identical culture conditions. Activation of all members of mitogen-activated protein kinases (MAPKs) was monitored by western immunoblot analysis. Expression levels of immediate/early genes c-fos and c-jun were assessed with quantitative real-time polymerase chain reaction. RESULTS Time course experiments revealed that both types of stresses activate the three members of MAPK, that is ERK, p38, and JNK, with cyclic stress exhibiting a slightly more extended activation. Further downstream, both stresses upregulate the immediate/early genes c-fos and c-jun, encoding components of the activator protein-1 (AP-1), a key transcription factor in osteoblastic ...
Over the last years, electronic cigarettes (ECs) have become more popular, particularly in individuals who want to give up smoking tobacco. The aim of the present study was to assess the influence of the different e-smoking liquids on the viability and proliferation of human periodontal ligament fibroblasts. For this study six test solutions with components from ECs were selected: lime-, hazelnut- and menthol-flavored liquids, nicotine, propylene glycol, and PBS as control group. The fibroblasts were incubated up to 96 h with the different liquids, and cell viability was measured by using the PrestoBlue® reagent, the ATP detection and the migration assay. Fluorescence staining was carried out to visualize cell growth and morphology. Data were statistically analyzed by two-tailed one-way ANOVA. The cell viability assay showed that the proliferation rates of the cells incubated with nicotine or the various flavored liquids of the e-cigarettes were reduced in comparison to the controls, though not all
Bone repair by periodontal ligament stem cell-seeded nanohydroxyapatite-chitosan scaffold Shaohua Ge,1 Ning Zhao,1 Lu Wang,1 Meijiao Yu,1 Hong Liu,2 Aimei Song,1 Jing Huang,1 Guancong Wang,2 Pishan Yang11Key Laboratory of Oral Biomedicine of Shandong Province, Department of Periodontology, School of Stomatology, 2Center of Bio and Micro/Nano Functional Materials, State Key Laboratory of Crystal Materials, Shandong University, Jinan, ChinaBackground: A nanohydroxyapatite-coated chitosan scaffold has been developed in recent years, but the effect of this composite scaffold on the viability and differentiation of periodontal ligament stem cells (PDLSCs) and bone repair is still unknown. This study explored the behavior of PDLSCs on a new nanohydroxyapatite-coated genipin-chitosan conjunction scaffold (HGCCS) in vitro as compared with an uncoated genipin-chitosan framework, and evaluated the effect of PDLSC-seeded HGCCS on bone repair in vivo.Methods: Human PDLSCs were cultured and identified, seeded on a
Background and Objective: Cell adhesion plays important roles in maintaining the structural integrity of connective tissues and sensing changes in the biomechanical environment of cells. The objective of the present investigation was to extend our understanding of the effect of cyclic mechanical strain on the expression of adhesion-related genes by human periodontal ligament cells. Material and Methods: Cultured periodontal ligament cells were subjected to a cyclic in-plane tensile deformation of 12% for 5s (0.2Hz) every 90s for 6-24h in a Flexercell FX-4000 Strain Unit. The following parameters were measured: (i)cell viability by the MTT assay; (ii)caspase-3 and -7 activity; and (iii)the expression of 84 genes encoding adhesion-related molecules using real-time RT-PCR microarrays. Results: Mechanical stress reduced the metabolic activity of deformed cells at 6h, and caspase-3 and -7 activity at 6 and 12h. Seventy-three genes were detected at critical threshold values <35. Fifteen showed a ...
1. Pihlstrom BL, Michalowicz BS, Johnson NW. Periodontal diseases. Lancet. 2005;366:1809-20 2. Khan SA, Kong EF, Meiller TF, Jabra-Rizk MA. Periodontal Diseases: Bug Induced, Host Promoted. PLoS Pathog. 2015;11(7):e1004952 3. Larsson L, Decker AM, Nibali L, Pilipchuk SP, Berglundh T, Giannobile WV. Regenerative Medicine for Periodontal and Peri-implant Diseases. J Dent Res. 2016;95:255-66 4. Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J. et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004;364:149-55 5. Nagatomo K, Komaki M, Sekiya I, Sakaguchi Y, Noguchi K, Oda S. et al. Stem cell properties of human periodontal ligament cells. J Periodontal Res. 2006;41:303-10 6. Bartold PM, Shi S, Gronthos S. Stem cells and periodontal regeneration. Periodontol 2000. 2006;40:164-72 7. Boisselier E, Astruc D. Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. Chem Soc Rev. 2009;38:1759-82 8. Khlebtsov ...
Eur J Pharmacol. 2011 Nov 16;670(1):295-303. doi: 10.1016/j.ejphar.2011.08.008. Epub 2011 Sep 2. Research Support, Non-U.S. Govt
TY - JOUR. T1 - Cyclic stretch force induces periodontal ligament cells to secrete exosomes that suppress IL-1β production through the inhibition of the NF-κB signaling pathway in macrophages. AU - Wang, Zhuyu. AU - Maruyama, Kentarou. AU - Sakisaka, Yukihiko. AU - Suzuki, Shigeki. AU - Tada, Hiroyuki. AU - Suto, Mizuki. AU - Saito, Masahiro. AU - Yamada, Satoru. AU - Nemoto, Eiji. PY - 2019/1/1. Y1 - 2019/1/1. N2 - In the oral mechanical environment, periodontal ligament cells (PDL cells) contribute to maintaining periodontal tissue homeostasis. Recent studies showed that exosomes, which are small vesicles secreted by various types of cells, play a pivotal role in cell-to-cell communication in biological processes. We examined the secretion of exosomes from PDL cells stimulated with cyclic stretch and their role in the inflammatory response of macrophages using the human macrophage cell line THP-1 and human primary monocytes/macrophages. We prepared supernatants from human PDL cells (PDL-sup) ...
Sigma-Aldrich offers abstracts and full-text articles by [Yoonsun Cha, Mijeong Jeon, Hyo-Seol Lee, Seunghye Kim, Seong-Oh Kim, Jae-Ho Lee, Je Seon Song].
Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line (2010 ...
Principal Investigator:KAWASE Toshio, Project Period (FY):1991 - 1992, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Functional basic dentistry
Define periodontal ligament nerve. periodontal ligament nerve synonyms, periodontal ligament nerve pronunciation, periodontal ligament nerve translation, English dictionary definition of periodontal ligament nerve. n. 1. Any of the cordlike bundles of fibers made up of neurons through which sensory stimuli and motor impulses pass between the brain or other parts of the...
Definition of fibrous, of periodontal ligament matrix in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is fibrous, of periodontal ligament matrix? Meaning of fibrous, of periodontal ligament matrix as a finance term. What does fibrous, of periodontal ligament matrix mean in finance?
BACKGROUND: Growth hormone (GH) is a potent regulator of bone formation. The proposed mechanism of GH action is through the stimulation of osteogenic precursor cell proliferation and, following clonal expansion of these cells, promotion of differentiation along the osteogenic lineage. OBJECTIVES: We tested this hypothesis by studying the effects of GH on primary cell populations of human periodontal ligament cells (PLC) and alveolar bone cells (ABC), which contain a spectrum of osteogenic precursors. METHODS: The cell populations were assessed for mineralization potential after long-term culture in media containing beta-glycerophosphate and ascorbic acid, by the demonstration of mineral deposition by Von Kossa staining. The proliferative response of the cells to GH was determined over a 48-h period using a crystal violet dye-binding assay. The profile of the cells in terms of osteogenic marker expression was established using quantitative reverse transcriptase polymerase chain reaction (RT-PCR) ...
Although previous studies have demonstrated that hydrogen sulfide (H2S) stimulated or inhibited osteoclastic differentiation, little is known about the effects of H2S on the differentiation of osteoblasts and osteoclasts. To determine the possible bioactivities of H2S on bone metabolism, we investigated the in vitro effects of H2S on cytotoxicity, osteoblastic, and osteoclastic differentiation as well as the underlying mechanism in lipopolysaccharide (LPS) and nicotine-stimulated human periodontal ligament cells (hPDLCs). The H2S donor, NaHS, protected hPDLCs from nicotine and LPS-induced cytotoxicity and recovered nicotine- and LPS-downregulated osteoblastic differentiation, such as alkaline phosphatase (ALP) activity, mRNA expression of osteoblasts, including ALP, osteopontin (OPN), and osteocalcin (OCN), and mineralized nodule formation. Concomitantly, NaHS inhibited the differentiation of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts in mouse bone marrow cells and blocked ...
The aim of this study was to observe the time-lapse changes in the rat periodontal ligament (PDL) during function and tooth movement. Under Nembutal anaesthesia, time-lapse changes in the thickness of the PDL of the first molars were investigated in five 12-week-old adolescent rats with microcomputed tomography. Three-dimensional (3D) images were reconstructed from the data. Histological observation was also performed, using undecalcified frozen sections of the maxillary first molar area.. The PDL appeared as a radiolucent furrow on the 3D images. A slight change in the thickness of the PDL was observed 1 hour after initiation of orthodontic force loading, which became significant after 6 hours, with the appearance of pressure-tension zones during the tooth movement. These changes were more significant 3 days after orthodontic loading.. Histological observation of the lingual cervical PDL (pressure zone) in nine 12- to 13-week-old rats demonstrated that the periodontal space had become narrow ...
Periodontal ligament stem cells (PDLSCs) represent a good source of multipotent cells for cell-based therapies in regenerative medicine. The success rate of these treatments is severely dependent on the establishment of adequate vasculature in order to provide oxygen and nutrients to the transplanted cells. Pharmacological preconditioning of stem cells has been proposed as a promising method to augment their therapeutic efficacy. In this study, the aim was to improve the intrinsic angiogenic properties of PDLSCs by in vitro pretreatment with deferoxamine (DFX; 100μM), fibroblast growth factor-2 (FGF-2; 10ng/mL) or both substances combined. An antibody array revealed the differential expression of several proteins, including vascular endothelial growth factor (VEGF) and placental growth factor (PlGF). ELISA data confirmed a 1.5 to 1.8-fold increase in VEGF for all tested conditions. Moreover, 48 hours after the removal of DFX, VEGF levels remained elevated (1.8-fold) compared to control conditions. FGF
Monkey periodontal ligament fibroblasts (MPLF cells), human gingival fibroblasts (HGF cells), rat embryonic calvaria cells (REC cells), porcine periodontal ligament epithelial cells (PPLE cells) and rat osteosarcoma 17/2 cells (ROS cells) were incorporated into 3-dimensional collagen gels plated in 60 mm Petri dishes in order: first, to measure the capacity of these cell types to contract; second, to investigate cell-collagen and intercellular relationships during contraction; and third, to define the cellular contribution to tissue contraction in an in vitro system. Measurements at times up to 72 h on 3 ml gels containing 5 × 10(5) cells and with a collagen concentration of 1.20 mg/ml showed that MPLF cells contracted the gels at a significantly greater rate (P less than 0.001) than did the other cell types. In addition, contraction started sooner and was of greater extent than with the other cells. HGF cells contracted the gels more rapidly than REC and PPLE cells, while ROS cells caused no ...
Recently, inflammation has been recognised as an important co-requisite to orthodontic tooth movement. When such a reaction is initiated, the process of up-regulation of certain adhesion molecules may occur, resulting in the extravasation of leukocytes. This may stimulate progenitor/precursor pathways and signals that regulate the biological responses resulting in tooth movement. We propose that up-regulation of leukocyte adhesion molecules occurs in response to orthodontic forces, resulting in circulating monocyte attraction, extravasation and differentiation into osteoclasts, which are responsible for bone resorption that results in orthodontic tooth movement. To investigate this hypothesis, it is necessary to determine whether periodontal ligament (PDL) endothelium responds to inflammatory stimuli as other organs do. We studied the normal distribution of endothelial adhesion molecule ICAM-1 within PDL vessels, and then the following exposure to an inflammatory endotoxin. The rat PDL blood vessels
Purpose/Aim: Cementogenesis is a critical step in periodontal tissue regeneration given the essential role of cementum in anchoring teeth to the alveolar bone. This study is designed to achieve integrated cementum formation on the root surfaces of human teeth using growth factor-releasing scaffolds with periodontal ligament stem/progenitor cells (PDLSCs). Materials and methods: Human PDLSCs were sorted by CD146 expression, and characterized using CFU-F assay and induced multi-lineage differentiation. Polycaprolactone scaffolds were fabricated using 3D printing, embedded with poly(lactic-co-glycolic acids) (PLGA) microspheres encapsulating connective tissue growth factor (CTGF), bone morphogenetic protein-2 (BMP-2), or bone morphogenetic protein-7 (BMP-7). After removing cementum on…. ...
Recently, research has focused intensely on age-related tissue changes, not only in the field of aesthetic dermatology but also in the dental field. Judging from clinical experience, relevant changes were suspected in the periodontal tissue thus influencing orthodontic tooth movement and the progression of periodontal disease.. Evidence was found that orthodontic tooth movement seems to be impaired in adult patients, thus requiring different treatment protocols than in adolescent patients. These findings will be discussed more thoroughly later in this article.. Periodontal disease however seems to be less dependent on age [1]. A study comparing experimental gingivitis in young vs. old individuals by Fransson et al. [2, 3] did confirm that older individuals experience more severe inflammation in response to experimental gingivitis. Nevertheless, several studies evaluating whether age is a risk factor for increased loss of periodontal support, have shown that aging either has no effect at all or, ...
The cells of cementum are the entrapped cementoblasts, the cementocytes. Each cementocyte lies in its lacuna, similar to the pattern noted in bone. These lacunae also have canaliculi or canals. Unlike those in bone, however, these canals in cementum do not contain nerves, nor do they radiate outward. Instead, the canals are oriented toward the periodontal ligament and contain cementocytic processes that exist to diffuse nutrients from the ligament because it is vascularized. After the apposition of cementum in layers, the cementoblasts that do not become entrapped in cementum line up along the cemental surface along the length of the outer covering of the periodontal ligament. These cementoblasts can form subsequent layers of cementum if the tooth is injured. Sharpey fibers are part of the principal collagenous fibers of the periodontal ligament embedded in the cementum and alveolar bone to attach the tooth to the alveolus.[2] ...
It is generally thought that the physical change of expanding the dental alveolar bone (socket) along with the severing of the periodontal ligament is the basis of a tooth extraction. Albeit that this does happen, an understanding of what is occurring biochemically is even more important. When the periodontal ligament is traumatized with forceps or elevators, hyaluronidase (hyaluronate glycanohydrolase) is released. This is an enzyme that catalyzes the hydrolysis of the interstitial barrier, hyaluronan (hyaluronic acid), which is the cement substance (extracellular matrix) of all human tissue. Once this chemical breakdown of the periodontal ligament by hyaluronidase is sufficient, the tooth is released from its attachment to the alveolus and can be removed.. This explains why the PHYSICS FORCEPS, with its steady unrelenting trauma to the periodontal ligament, quantitatively creates a greater release of hyaluronidase in a shorter period of time than traditional forceps or elevators extractions ...
Periodontitis is a chronic destructive category of periodontal disease that progresses to the resorption of alveolar bone, which leads to progressive bone destruction and tooth loss. As a consequence of resorption, breakdown of products are released into periodontal tissues, migrating toward the gingival sulcus and gathering from the surrounding site in whole saliva, where several of them have been identified. 2930. Among the several host enzymes proposed as diagnostic indicators of periodontal status, ALP was one of the first to be identified. 31 ALP is released from polymorphonuclear cells (PMNs) during inflammation 10 and from osteoblasts 32 and periodontal ligament fibroblasts 33 during bone formation and periodontal regeneration respectively.. ALP activity in serum has been extensively studied, and it was suggested that ALP allows bone mineralization by releasing an organic phosphate that contributes to the deposition of calcium phosphate complexes into the osteoid matrix. 3435 ALP might ...
THE ANATOMICAL RECORD 222:191-200 (1988) Radioautographic Demonstration of Receptors for Epidermal Growth Factor in Various Cells of the Oral Cavity MOON-IL CHO, YU LIN LEE, AND PHILIAS R. GARANT Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, New York 11794-8700 ABSTRACT Mouse iodinated epidermal growth factor (EGF)was localizedby light and electron microscopic radioautography in basal cells of oral epithelium, papillary cells of the enamel organ, periodontal ligament fibroblasts, preodontoblast precursor cells, and preosteoblasts of the alveolar bone of 13-day-old Sprague-Dawleyrats. The specificity of binding in these cells was suggested by an observed reduction of about 90% in the labeling when excess unlabeled EGF was injected along with the 125I-EGF. In contrast, fully differentiated cells, such as ameloblasts. odontoblasts, and osteoblasts, were only poorly labeled. Quantitative analysis of the light microscopic ...
Primary Tooth A primary tooth that has been avulsed is usually not reimplanted. The risk of injury to the developing permanent tooth bud is high. Permanent Tooth Do not touch the root of the tooth. Handle the tooth by the crown only. Rinse the tooth off only if covered by debris. Do not scrub or scrape the tooth. Attempt to reimplant the tooth into the socket with gentle pressure, and hold it in position. Hold the tooth in place. Aluminium foil may be used to help stabilise the tooth, or the patient can bite gently on gauze or a soft cloth. Time is critical and immediate replacement is best, and ideally should not be delayed more than 30 minutes. If unable to reimplant the tooth, place it in a protective transport solution, such as Hanks solution, milk, saline, or placing it in the patients mouth next to the cheek, if the patient is able to do so. This will hydrate and nourish the periodontal ligament cells which are still attached to the root. A small container of Hanks Balanced Salt ...
Background. Stem cells have contributed to the development of tissue-engineered-based regenerative periodontal therapies. In order to find the best stem cell sources for such therapies, the biologic properties of stem cells isolated from periodontal ligaments (PDL) of deciduous (DePDLSC) and permanent (PePDLSC) teeth were comparatively evaluated. Methods. PDL stem cells were isolated from six sound fully erupted premolars and six deciduous canines of healthy subjects. In vitro biologic characteristics such as colony formation, viability, stem cell marker identification and osteogenic differentiation (using alkaline phosphatase analysis and Alizarin red staining) were comparatively assessed using one-way ANOVA and post hoc Tukey tests using SPSS 13 ...
Tissue sections showed that the replanted tooth was surrounded by newly formed, functional periodontal ligament fibres and new cementum, the essential ingredients of a healthy tooth attachment. In contrast, tooth molars that were replanted without new stem/progenitor cells were either lost or loosely attached and were resorbed, the scientists found. The study, published in an online issue of the journal Tissue Engineering, was funded through a grant by the National Institutes of Health. The natural surface of the tooth played an essential role in the study. To verify that the ligament was formed by the transplanted stem cells and not by the animals own cells, stem cells were labelled with green fluorescent protein prior to seeding them on the molars and re-inserting the teeth into the animals mouth. According to Tom Diekwisch, director of the Brodie Laboratory and senior author on the paper, this is the first progenitor cell-based regeneration of a complete periodontal ligament in which a ...
The composition of the collagen network in which the cells are located has a considerable influence on the contractile properties of the cells. Contraction of the cells creates internal tension in the network which keeps the cells active. The network must be well attached to the surrounding solid surfaces to prevent its detachment. Berendsen managed to attach the network to these artificial root and bone surfaces present in the model by creating an enzyme-mediated mineral deposition on the surfaces. By subsequently applying loading to the tooth root (mimicking the process of chewing) in the 3D model, she was able to deform the mineral-anchored network containing the cells. The subsequent response of the cells was dependent on the magnitude of the loading ...
Principal Investigator:Yoshiki Nakamura, Project Period (FY):2015-04-01 - 2018-03-31, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Orthodontics/Pediatric dentistry
Periodontal regeneration - true regeneration of the attachment apparatus (new cementum, new periodontal ligament, and new alveolar bone) on a previously diseased root surface when used specifically in the LANAP® Protocol. 510(k) ...
Teeth are supported by the surrounding peridontium, which is composed of both soft (periodontal ligament and lamina propria) and hard connective tissue (cementum and bone).. It is the periodontium that provides attachment of the teeth to the jaw and, when the periodontal tissues become diseased, the resultant lack of support results in tooth loss. Age is a definite factor in such loss, although other obvious causes are decay and physical trauma.. The loss of teeth decreases the preservation of an individuals dentition and also detracts considerably from a socially acceptable appearance. In such cases, the replacement of missing teeth is accomplished by the fabrication of either a complete or partial denture, or both. Regrettably, dental care in the elderly population is often neglected. This may be due to other physical handicaps, which may restrict the patients ability to travel or in situations where the patients are confined to their homes.. ...
Teeth are supported by the surrounding peridontium, which is composed of both soft (periodontal ligament and lamina propria) and hard connective tissue (cementum and bone).. It is the periodontium that provides attachment of the teeth to the jaw and, when the periodontal tissues become diseased, the resultant lack of support results in tooth loss. Age is a definite factor in such loss, although other obvious causes are decay and physical trauma.. The loss of teeth decreases the preservation of an individuals dentition and also detracts considerably from a socially acceptable appearance. In such cases, the replacement of missing teeth is accomplished by the fabrication of either a complete or partial denture, or both. Regrettably, dental care in the elderly population is often neglected. This may be due to other physical handicaps, which may restrict the patients ability to travel or in situations where the patients are confined to their homes.. ...
Used to sever the periodontal ligament prior to an extraction in order to preserve surrounding bone and tissue.|ul||li|Blade shape is slightly rounded and paper thin to slide easily below the gumline|/li||li|Blades are flexible so there is less chance of
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Looking for online definition of Malassez epithelial rests in the Medical Dictionary? Malassez epithelial rests explanation free. What is Malassez epithelial rests? Meaning of Malassez epithelial rests medical term. What does Malassez epithelial rests mean?
The therapeutic strategies for neurodegenerative diseases still represent a vast research field because of the lack of targeted, effective and resolutive treatment for neurodegenerative diseases. The use of stem cell-based therapy is an alternative approach that could lead to the replacement of damaged neuronal tissue. For this purpose, adult mesenchymal stem cells (MSC), including periodontal ligament stem cells (PDLSCs), could be very useful for their differentiation capacity, easy isolation and the ability to perform an autologous implant. The aim of this work was to test whether the Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate; GMG-ITC], an isothiocyanate extracted from Moringa oleifera seeds, was able to induce PDLSCs toward neural progenitor differentiation. Next-generation transcriptomics sequencing showed that moringin treatment increased the expression of genes involved in neuron cortical development and in particular in neuron belonging to upper and deep cortical layers. ...
Looking for online definition of periodontal ligament in the Medical Dictionary? periodontal ligament explanation free. What is periodontal ligament? Meaning of periodontal ligament medical term. What does periodontal ligament mean?
Fingerprint Dive into the research topics of Hypotonic stress induces RANKL via transient receptor potential melastatin 3 (TRPM3) and Vaniloid 4 (TRPV4) in human PDL cells. Together they form a unique fingerprint. ...
Cytotoxicity of a novel nano-silver particle endodontic irrigant Eric LK Chan,1 Chengfei Zhang,2 Gary SP Cheung2 1Department of Health, Government of Hong Kong SAR, Hong Kong, Special Administrative Region; 2Comprehensive Dental Care (Endodontics), Faculty of Dentistry, University of Hong Kong, Hong Kong Special Administrative Region of the People's Republic of China Purpose: The aim of this study was to evaluate the cytotoxic effect of a novel nano-silver particle (25.2±6.5 nm) endodontic irrigant (0.2 mM) and compare it with 3% sodium hypochlorite. Materials and methods: Two cell types, mouse fibroblast National Institutes of Health 3T3 (NIH 3T3) and primary human periodontal ligament stem cell (hPDLSCs) were used in a test for the effect of direct and indirect (by separating the agent and cell with a layer of agar) exposure to the two solutions. In the direct exposure experiment, ten groups of cell cultures were exposed to one dilution (3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6 or 1:7) of a
TY - JOUR. T1 - Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. AU - Lee, Hyo Seol. AU - Lee, Jongeun. AU - Kim, Seong Oh. AU - Song, Je Seon. AU - Lee, Jae Ho. AU - Lee, Syng Ill. AU - Jung, Han Sung. AU - Choi, Byung Jai. PY - 2013/12/23. Y1 - 2013/12/23. N2 - The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. The gene-expression profiles of the dental follicle and PDL were compared using the cDNA microarray technique. Microarray analysis identified 490 genes with a twofold or greater difference in expression, 365 and 125 of which were more abundant in the dental follicle and PDL, respectively. The most strongly expressed genes in the dental follicle were those related to bone development and remodeling (EGFL6, MMP8, FRZB, and NELL1), apoptosis and chemotaxis (Nox4, CXCL13, and CCL2), and tooth and embryo development (WNT2, PAX3, FGF7, AMBN, AMTN, and SLC4A4), ...
It is well-documented that both chemokine (C-C motif) ligand 19 (CCL19) and 21 (CCL21) mediate cell migration and angiogenesis in many diseases. However, these ligands precise pathological role in ankylosing spondylitis (AS) has not been elucidated. The objective of this study was to examine the expression of CCL19 and CCL21 (CCL19/CCL21) in AS hip ligament tissue (LT) and determine their pathological functions. The expression levels of CCL19, CCL21 and their receptor CCR7 in AS (n = 31) and osteoarthritis (OA, n = 21) LT were analyzed via real-time polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC). The expression of CCL19, CCL21 and CCR7 in AS ligament fibroblasts was also detected. The proliferation of ligament fibroblasts was measured via a cell counting kit-8 (CCK8) assay after exogenous CCL19/CCL21 treatment. Additionally, the role of CCL19/CCL21 in osteogenesis was evaluated via RT-PCR and enzyme-linked immunosorbent assay (ELISA) in individual AS fibroblast cultures. Furthermore,
The lateral periodontal cyst is a non-inflammatory developmental cyst that arises from the epithelial post-functional dental lamina, which is a remnant from odontogenesis. It is more common in middle-aged males. Usually asymptomatic, it presents as a regular well-corticated radiolucency on the side of a mandibular canine or premolar root. Histologically, the cyst appears similar to the gingival cyst of the adult, having a non-keratinized squamous epithelial lining. The involved tooth is usually vital and has no indication for root canal treatment unless the signs of non-vital or necrotic pulpal tissue were confirmed. The cysts arise from epithelial rest cells in the periodontal ligament, although it is unknown whether from the cell rests of Malassez, reduced enamel epithelium or dental lamina remnants, and are generally treated by surgical enucleation. Altini M, Shear M. The lateral periodontal cyst: an update. J Oral Pathol Med. 1992 Jul; 21(6):245-50. Wood K, Goaz P. 5th ed. St. Louis: Mosby; ...