Periodic acid-Schiff (PAS) is a staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. The reaction of periodic acid oxidizes the vicinal diols in these sugars, usually breaking up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of the monosaccharide units that are parts of the long polysaccharides, and creating a pair of aldehydes at the two free tips of each broken monosaccharide ring. The oxidation condition has to be sufficiently regulated so as to not oxidize the aldehydes further. These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen. Alcian blue/periodic acid-Schiff (AB/PAS or AB-PAS) uses alcian blue before the PAS step. PAS staining is mainly used for ...
Diastase Kenei is a medicine available in a number of countries worldwide. A list of US medications equivalent to Diastase Kenei is available on the Drugs.com website.
FLORKEGERLOFF S, KRAUSE W, TOPFERPETERSEN E, Tschesche H, MULLERESTERL W, ENGEL W. ON THE TERATOGENESIS OF ROUND-HEADED SPERMATOZOA - INVESTIGATIONS WITH ANTIBODIES AGAINST ACROSIN, AN INTRAACROSOMALLY LOCATED ACROSIN-INHIBITOR, AND THE OUTER ACROSOMAL MEMBRANE. ANDROLOGIA. 1985;17(2):126-138 ...
TY - JOUR. T1 - An approach to immunomicroscopy of renal disease with immunoperoxidase and periodic acid-Schiff counterstain (IMPAS stain). AU - Tubbs, R. R.. AU - Gephardt, G.. AU - Valenzuela, R.. AU - Deodhar, S.. PY - 1980/1/1. Y1 - 1980/1/1. N2 - A series of 127 renal specimens reflecting a wide spectrum of renal pathology was evaluated with a direct immunoperoxidase technic in which frozen tissue was first reacted with anti-Ig(G,A,M) and anti-C3-peroxidase conjugates and subsequently counterstained with periodic acid-Schiff-hematoxylin (IMPAS). This technic permitted simultaneous evaluation of light-microscopic changes and yielded a permanent immunomicroscopic record. Frequently, a distinction could be made between epimembranous, intramembranous and subendothelial deposits, which was not possible using immunofluorescence. The findings were correlated with histologic, immunofluorescent and electronmicroscopic results. General patterns of immune complex and autoantibody deposition were ...
On electron microscopy (EM), there have been no electron-dense deposits no viral contaminants were noticed. (This case continues to be briefly referred to with focus on kidney biopsy results, as part of kidney biopsy series.7) Open in a separate window Figure 1 The kidney biopsy findings. (A) Two glomeruli in the center reveal crescents - a cellular crescent in the glomerulus to the left and a fibrocellular crescent to the right; surrounding tubules reveal distension and flattening of the epithelium (Periodic acid-Schiff stain, 200x). (B) Immunofluorescence staining for IgG reveals no significant staining in depicted glomerulus or surrounding tubular basement membranes (FITC, 200x). (C) Representative section showing negative immunohistochemistry staining for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein after antigen retrieval (200X). (D) Lung tissue from a known SARS-CoV-2 infected patient served as positive control for immunohistochemistry method (400X) ...
Background Self-administered swabs are used to sample vaginal contents for a variety of clinical purposes including detection of sexually transmitted infections, condom breakage, and vaginal product use. The goal of this study was to determine whether a quantitative glycogen assay can be used to assess whether a swab has been exposed to the vagina to assure study compliance. Study Design Buccal, skin, or vaginal samples were tested to determine whether a commercial quantitative glycogen assay can differentiate vaginal specimens. In addition, archived remnant de-identified vaginal swabs from clinical trials were tested. Periodic acid-Schiff stain was used to identify glycogen-positive cells as a confirmation test. Results Glycogen concentrations in eluates of vaginal swabs from reproductive-aged women were significantly higher than those from unused swabs (mean ± SE, 964 ± 135 μg/mL vs. 14.7 ± 2.5 μg/mL, P , 0.001) and swabs exposed to buccal and finger/hand epithelia (40.3 ± 4.8 and 18.5 ...
BACKGROUND: From the developmental aspects, the distribution of fluorescein isothiocyanate dextran 70,000 (FTTC-dextran) and mucous gel across the lumen of small intestine was observed as an investigation into the role of mucous gel on intestinal permeability. Furthermore, the effect of N-acetyl cysteine (NAC), a mucolytic agent, on intestinal permeability was examined. METHODS: In suckling and weaned rats, FTTC-dextran (750 mg/kg body wt) was gavage-fed. After 3 hours, blood samples were taken by cardiac puncture to analyze plasma FTTC-dextran by fluorescence spectrometry. Samples of small intestine with luminal contents were frozen and sectioned in a cryostat for fluorescence microscopy; the same sections were placed in a 0.2% celloidin solution to preserve mucous gel and were stained by periodic acid-Schiff reaction for light microscopy. In weaned rats, intestinal permeability was examined with different concentrations of intraluminally instilled NAC. RESULTS: The plasma level of FTTC-dextran ...
Images: Cuboidal lining , Glycogen , Aggressive serous cyst , Cysts in serous cystadenomas are composed of a single layer of clear cuboidal cells. These cells usually form flat sheets that line the cysts and only rarely form papillae that project into the cystic spaces. The neoplastic cells have clear cytoplasm because they contain abundant intracytoplasmic glycogen. They do not produce mucin. The nuclei are small, round, and have uniform chromatin and inconspicuous nucleoli. Atypia and mitoses are essentially absent. The central stellate scar and the stroma separating the cysts are composed mostly of relatively acellular collagenous connective tissues, but the stroma may contain entrapped islets of Langerhans and acini. Special stains will highlight the abundant glycogen and absence of mucin. A periodic acid Schiff (PAS) stain will be strongly positive and sensitive to diastase digestion. Stains for mucin, including a mucicarmine and alcian blue stains, will be negative, as will stains for ...
TY - JOUR. T1 - Mucin staining is of limited value in addition to basic immunohistochemical analyses in the diagnostics of non-small cell lung cancer. AU - Micke, Patrick. AU - Botling, Johan. AU - Mattsson, Johanna Sofia Margareta. AU - Planck, Maria. AU - Tran, Lena. AU - Vidarsdottir, Halla. AU - Nodin, Björn. AU - Jirström, Karin. AU - Brunnström, Hans. PY - 2019/12/1. Y1 - 2019/12/1. N2 - Accurate diagnosis of histological type is important for therapy selection in lung cancer. Immunohistochemical (IHC) and histochemical stains are often used to complement morphology for definite diagnosis and are incorporated in the WHO classification. Our main aim was to compare different mucin stains and assess their value in relation to common IHC analyses in lung cancer diagnostics. Using tissue microarrays from 657 surgically treated primary lung cancers, we evaluated the mucin stains periodic acid-Schiff with diastase (PASD), alcian blue-periodic acid-Schiff (ABPAS) and mucicarmine, and compared ...
Hi All: > > I am in the process of working up a PAS Staining protocol. The PAS stain > is working fine, but I am unable to get my tissue to digest. I am using > .5g of Diastase in 50ml of DI water. I tried heating the solution in a > water bath for 30 min, 1 hour and 1.5 hours and it still looks the same > as the one without digestion. Could I be using the wrong Diastase? If > so, does anyone have any suggestions for a new product? > > Thanks, > > Tanisha Neely, HT(ASCP) > AP-Histology/Specimen Management > > > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in ...
Zym information about active ingredients, pharmaceutical forms and doses by Biosans Lifecare, Zym indications, usages and related health products lists
Macroscopically, the tumor was of yellow-greyish color and medium consistency. Histologic examination of both the primary and recurrent tumors showed solid nests of tumor cells surrounded by a small rim of connective tissue. The neoplasm consisted of polygonal cells with abundant, eosinophilic, granular cytoplasm and relatively sharp cellular margins. Cytoplasmic granules were distinctly PAS-positive and resistant to diastase digestion. There was remarkable cellular and nuclear polymorphism with hyperchromatic nuclei and prominent nucleoli (Fig. 2).There were small areas of foamy cells and perivascular lymphocytic aggregates. Mitoses were rarely registered. The tumor was moderately vascularized. Frank necrosis was not evident. Immunohistochemically, tumor cells were strongly positive for neuron-specific enolase, S-100 protein, a1-antitrypsin and a1-antichymotrypsin (Fig. 3), but negative for glial fibrillary acidic protein, vimentin, various cytokeratins, melanoma marker HMB 45, chromogranin A, ...
... is the most versatile and widely used technique for carbohydrate visualization. From a diagnostic point of view...
The patterns seen using the PAS reaction were different from the patterns seen following staining for F8. In particular, the vascular loops and networks patterns were not seen in the F8 stained sections, and it is these two patterns that were demonstrated to be of prognostic importance.1-3 The pattern of autofluorescence closely resembled the pattern seen with the PAS reaction (see Figs2, 3, 4) and it is very likely that the fluorescent patterns observed by Folberg et al,1 in their fluorescein conjugated UEA-1 stained sections, were mainly the result of tissue autofluorescence. This conclusion is further reinforced by their observation that these fluorescent patterns could partially resist bleaching1 whereas we have noted that staining with UEA-1 is abolished by bleaching.15 The autofluorescence resists bleaching.. Autofluorescence can also be seen from the septa of the optic nerve (present in some of the uveal melanoma sections) where there can be little doubt that it is connective tissue, ...
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.. Learn More ...
JOINT synovial fluid is considered to be a dialysate of blood with added mucin, which consists of hyaluronic acid1. The source of the latter is uncertain, but the belief is current that the synovial membrane is the likely site of formation. Articular cartilage is said to contain no hyaluronic acid, and the sulphated mucopolysaccharide it does contain is not found in appreciable quantity in synovial fluid. Synovial membrane is not lined by an epithelium, but is composed of fine, compact connective tissue of variable cellularity2, the cells being indistinguishable from fibroblasts by electron microscopy3. Claims have been made that the membrane, especially in its superficial part, stains with mucicarmine4-6, and also reacts with the periodic acid - Schiff technique3. Metachromasia, which might indicate the presence of hyaluronic acid in the membrane, has not been unequivocally demonstrated; some claim that the membrane is metachromatic6,7, but others deny it3,5.
18122-72-0 - XPMRNVZJQGDGFS-UHFFFAOYSA-I - Periodic acid (H5IO6), sodium salt (1:5) - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Sections of a gastrula-stage mouse embryo are stained with periodic acid-Schiff reagent and counterstained with methylene blue. Which of the following families of proteins forms anchoring junctions between adjacent epithelial cells in the neural ectoderm of this embryo (arrow, shown in the image)? (A) Pseudostratified cuboidal (B) Simple columnar (C) Simple cuboidal (D) Simple squamous (E) Stratified columnar 6 A portion of the upper esophagus, collected at autopsy, is fixed with formalin, embedded in paraffin, sectioned at 6 μm, stained with H&E, and examined by light microscopy (shown in the image). Adipocytes, nerves, and muscle cells secrete a pericellular matrix that is referred to as lamina externa (choice E). Keywords: Epithelial tissue, cell polarity 2 The answer is C: Simple cuboidal. This image shows parallel rows of cuboidal epithelial cells lining collecting ducts in the medulla of the kidney. The cells are more or less square (cuboidal) with central basophilic nuclei. The ...
Luxol Fast Blue - PAS Stain Kit is designed based on Luxol fast blue and Periodic acid-Schiff (PAS) method.is made in a ready-to-use format and offers high quality, rapid staining of myelin/myelinated axons
Conjunctival cytology analysis (stained with periodic acid-Schiff, 40×).Notes: (A) refers to the staining before injection. (B) represents the staining 1 mon
Plant enzymes have a wider range of activity than animal enzymes because there are so many of them such as protease, peptidase, lipase, amylase, glucoamylase, alpha-galactosidase, cellulase, hemicellulase, invertase, malt diastase, lactase, pectinase, and phytase. Wow, thats a mouthful of "-ases.". The plant enzymes, which are in your yellow capsule of Essentialzymes-4, contain Fiberzyme, Polyzyme, and Carbozyme and are so essential for you.. Why is Fiberzyme important? People today do not digest fiber, and fiber is the host to candida, bacteria, and viruses. I have yet in 30 years to see a single patient with candida who was able to digest fiber.. Polyzyme contains protease to maximize protein digestion. It facilitates the breakdown of proteins into amino acids, supports the digestion of high-fat foods such as nuts, and enhances the absorption of nutrients.. Carbozyme contains amylase for digesting carbohydrates. The absence of amylase is the No. 1 cause of allergies today, so look at that and ...
Hey everyone today is my first time on Whole30 and I really need it. My youngest daughter is 7 months today and just starting food. I had a diastases recti after her birth and its made moving around quite a slow process. I started her pregnancy carrying 10 extra pounds than I normally do and since shes been born Ive put on another 10 pounds , basically losing a bit of baby weight after the birth and losing none of the pregnancy weight and gaining even more. Needless to say sleep is a challenge therefore my energy is super sporadic depending on my night. Cravings for sugar and carbs and alcohol have been rampant. So I knew a big change needed to be made if I wanted my situation to change. Ive been making changes towards eating like the program leading up to the start of the program, like no more cream and sugar in my coffee, reducing simple carbohydrates and all process foods. Cut out alcohol and reducing dairy and Ive lost 5lbs before I even began. I also just signed up for RealPlana and I ...
Near rhymes (words that almost rhyme) with nowadays: diastase, phosphatase, plagioclase, hollandaise... Find more near rhymes/false rhymes at B-Rhymes.com
Liquid; Oral; Diastase 0.2 g Pepsin 25 mg; Vitamin B1 5 mg; Vitamin B12 7.5 mg; Vitamin B2 2.5 mg; Vitamin B3 (Niacinamide) 22.5 mg; Vitamin B5 (D-Panthenol) 5 mg; Vitamin B6 1 mg / 15 ...
Full spectrum of dairy digestive enzymes. Plus CereCalase, a special enzyme blend of phytase, hemicellulase and beta-glucanase. Available in easy-to-swallow vegetable capsules for maximum assimilation and absorption.Supplement FactsServing Size: 1 CapsuleLactase (5000 ALU) 50 mg.Protease (10000 HUT) 17 mg.Invertase (0.25 IAU) 13 mg.Amylase (1000 DU) 10 mg.Lipase (200 FCC LU) 10 mg.CereCalase (150 MU) 8 mg.Glucoamylase (2 AGU) 4 mg.Malt Diastase (100 DP) 4 mg.Other Ingredients: 100% Kosher vegetable capsules, vegetable cellulose, rice bran, water.
This is a proposed rule amending the NASA FAR Supplement (NFS) to specify that use of a priority rating under the Defense Priorities and Allocations System (DPAS) regulation is not required unless the acquisition is in one of the Schedule L categories of the DPAS.
Like OCIAD1, OCIAD2 is a cancer-related protein and its expression level increases during the course of malignant progression and is thought to be a very useful marker for evaluating the malignancy of ovarian mucinous tumors ...
In the present study, we detected the presence of abnormal mitochondria with degenerated inner membranes from a young age in mice with KO of the group VIA phospholipase A2 (iPLA2β) gene. The collapsed and degenerated mitochondria were accompanied by focal axonal degeneration, and the plasma membranes were also degenerated at the axon terminals. Thus, two kinds of degenerative membranes in axons appeared to be intrinsically associated with the pathomechanism of neuroaxonal dystrophy in iPLA2β-KO mice.. In INAD, spheroids often contain PAS-positive granular material as well as membranous material (Cowen and Olmstead, 1963; Mahadevan et al., 2000), similar to those in iPLA2β-KO mice. The PAS-positive granules in iPLA2β-KO mice were identified ultrastructurally as abnormal mitochondria with degenerated inner membranes. Absence of immunoreactivity for CCO would have been attributable to degeneration of cristae, as reported previously in mice (Kirkinezos et al., 2005). Because PAS stains ...
A case of pseudofungi in axillary lymph nodes is presented. They resembled septate hyphae and were stained with a periodic acid-Schiff stain. Gomori methenamine silver stain failed to stain them. Because of morphologic similarity to true septate fung
Results CA occurred in 13/35 GILM (37%) from the following sites: oesophagus (4/8), stomach (5/7) including one frozen section, small intestine (1/2) and large intestine (3/18), but were not identified in 19 gastrointestinal stromal tumours (12 gastric, 7 small intestinal; p=0.0019), five schwannomas (three gastric, two small intestinal; p=0.154) and 35 non-GILM (p=0.0001). The densities of CA ranged from one per 4-200 mm2. CA stained intensely with periodic acid Schiff after diastase predigestion (PASD), Alcian blue and ubiquitin, and faintly in peripheral zones for desmin and smooth muscle actin. Ultrastructurally, CA consisted of an electron-dense outer layer and a fibrillar core with scattered particle matter. HG were present in all leiomyomas, but showed variable staining for PASD, negative staining for Alcian blue and ubiquitin, and positive staining for smooth muscle markers.. ...
Digestolax information about active ingredients, pharmaceutical forms and doses by Les Laboratoires Bio-Sante, Digestolax indications, usages and related health products lists
To test the suggestion that an inherited defect in colonic mucus rendering it susceptible to degradation by bacterial enzymes may be an important factor in the aetiology of ulcerative colitis, 650 colonoscopic and rectal biopsy specimens from 166 patients with colitis were stained by mild periodic acid Schiff (mPAS), which shows sialic acid that is deficient in O-acetyl substituents. There was an excess of mPAS positive sialic acid in patients with chronic ulcerative colitis, but the increased expression was patchy and coincided with a morphological change in the form of epithelial hyperplasia (metaplasia). Hyperplasia was more common in the rectum and in women and was associated with, and presumably secondary to, active inflammation. It is concluded that variation in the structure of sialic acid is acquired and is therefore unlikely to be implicated in the aetiology of ulcerative colitis.
1. Introduction. Microvillous inclusion disease (MVD) or microvillous atrophy disorder is a congenital disorder of the small intestinal epithelial cells that presents with persistent and severe diarrhea and it is characterized by enterocyte abnormalities [1] . The diarrhea starts in the first 72 hours of life (early onset form) or in 6 to 8 weeks after birth (late onset) [2] . This inheritance of MVD appears to be autosomal recessive, based on cases occurring in siblings and high incidence of consanguinity [3] . Molecular studies demonstrate mutation of the MYO5B that encoded for myosin 5b has a role in pathogenesis of the MVD [4] . Diagnosis is often delayed because of difficulties in taking a small bowl biopsy specimen in the neonatal period. Light microscopy shows enteropathy, and severe atrophy of the enterocytes brush borders, with instead of, accumulation of Periodic Acid Schiff (PAS) and CD10 positive granules at the apical pole of immature enterocytes [1] . Transmission electron ...
The relationship between the levels of renalase and changes in proteinuria, hypertension, renal function, renal tubular epithelial cell apoptosis and B-cell lymphoma-2 (Bcl-2) expression was investigated in patients (chronic nephritis, primary nephrotic syndrome or other kidney disease) that underwent renal biopsy. The study group comprised 72 patients undergoing renal biopsy. Patient profiles and renal function were collected. Concentrations of renalase and Bcl-2 were measured by immunohistochemistry. Tubular injury was detected by periodic acid Schiff staining (PAS) and renal tubular epithelial cell apoptosis was assessed by TUNEL assay. The expression of renalase was significantly lower in renal biopsy specimens than in normal kidney tissues. There was a positive linear relationship between renalase and some serum and cardiac indices; a negative correlation was found between age, eGFR, Ccr and 24-h urinary protein. Renal tubule injury index and tubular epithelial cell apoptosis index showed a ...
Newcomer Supply Alpha Amylase 1%, Aqueous is a convenient ready-to-use glycogen digestion solution for aiding in further identification of mucosubstances used in conjunction with the Periodic Acid Schiff (PAS) Stain procedure.
I know that the reaction proceeds by forming a cyclic diester of periodic acid followed by cleavage of $\ce{C-C}$ bond and formation of $\ce{C=O}$ bond. But how do we compare the rates of the given compounds?. My first thought was to think $\ce{C-C}$ cleavage as formation of a pseudo carbocation which would be stabilised by +I effect of the alkyl groups and also the hyperconjugation, but I dont think it is right.. ...
Perjodic Acid Schiff Reaction:. Structures containing carbohydrates, like glycogen and reticular fibres containing lots of glycoprotein, basal membranes, and mucins stain pink to violet. Perjodic acid splits the carbon bonds between two neighboring secondary alcohols. Then the alcohol groups are oxidized to aldehydes and are stained reddish by Schiff reagent.. ...
Acoustic energy absorbed by tissue and thereby converted to heat induces coagulation necrosis within the focus. The morphological characteristics of the lesion change with the applied energy and the time of follow-up.. Immediately after ultrasound exposure with low energy levels, the lesion sometimes cannot be detected macroscopically. Even microscopy only shows an area that is less strongly stained by periodic acid-Schiff, without changes in the cellular structure [38,39]. Ultrastructural examination of the kidney has revealed damage to organelles within the first couple of hours. The initial healing process indicated the presence of these discrete lesions. Medium energy levels induced a sharp lesion that was macroscopically detectable 1 h after HIFU treatment and that was demarcated within the next few days [40]. Focusing on the renal corti-comedullary border resulted in pronounced streaky bleeding of the medulla. Macroscopic and microscopic lesions appeared to be less extensive in the cortex. ...
This is a histology slide of vaginal tissue from a rat. Tissue was fixed in 10% neutral buffered formalin and embedded in paraffin. Deparaffinized tissue sections (5 µm) was stained with periodic acid-Schiff (PAS ...
Porcine oral mucosa specimens were treated by a conventional scalpel, different laser wavelengths (Er:YAG, CO2, diode and Nd:YAG), high frequency surgery (hf1 SURG®, Vio® 300 D) and ultrasonic activated scalpels (UltraCision®, SonoSurg®) with evidence based settings. All specimens were fixed in 10% buffered formalin solution and embedded in paraffine. Sections were stained with hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) reaction and examined light microscopically by two independent pathologists. The incisions were evaluated measuring the cutting efficacy, the width of damaged tissue adjacent to the incision and the quality of tissue and cells in the damaged areas. Finally the researched dates were described using synoptic gradings ...
Innocent A. Edagha, Nauclea latifolia Ethanolic Leaves Extract Moderately Hepatoprotects, but Decreases Periodic Acid-Schiff and Up-regulates Cytokeratin-7 Expression in Prophylaxis Malaria, International Journal of Sciences 05(2017):66-72 DOI: 10.18483/ijSci. ...
The hypothesis predicted that the amylum would be fastest digested at the highest sourness degrees. This was based on the cognition that acid reacts fast and is caustic. This was proven to be wrong as the fastest recorded clip ( mean ) was 65seconds with a pH of 4. followed by pH7 and so pH9. The enzyme that was used in the experiment is called diastase and is found in the organic structure in spit. Saliva has a pH of about 5. 6 to 7. 9. ( livestrong. com. 2012 ) This can change based on diet. Therefore the achieved consequences are 1. 6pH below that of the existent enzymes used by the organic structure itself when digesting amylum.. It was non expected that the pure acid buffer ( pH 1 ) would ensue in the slowest reaction of Al at 173seconds ( norm ) . even slower than that of a complete alkaline solution ( pH 14: 157 seconds ) . A more accurate dependable consequence could be attained. if the experiment were to be repeated legion times. other minor facets could besides be improved. ( refer to ...
Introduction. The Effect that Temperature Has on the Rate at which the Enzyme (Amylase) can Breakdown its Substrate Aim: The aim of this experiment is to find out the effect that the temperature has on the rate at which the enzyme (Amylase) can breakdown its substrate, which is Starch. Amylase: Enzyme having physiological, commercial, and historical significance, also called diastase. It is found in both plants and animals. Amylase hydrolyzes starch, glycogen, and dextrin to form in all three instances glucose, maltose, and the limit-dextrins. Prediction: I predict that as the temperature increases, the speed of the reaction will increase. Since temperature is a measure of the motion of particles, increasing the temperature will cause the particles to move faster. When particles move faster, more collisions occur and the collisions are more violent. This should increase the reaction rate. This is backed up by the collision theory, according to this theory; reacting molecules must collide with ...
A 83-year-old woman had a 2.5X1.5 cm hyperkeratotic plaque covered with some crusts, and two reddish nodules were noticed near the center of the lesion. This plaque had been noticed over her right cheek for one year. Histopathological examination reveals that tumor cells showed a lobular proliferation in continuity with the epidermis. Tumor nests were mostly composed of large atypical cells with clear cytoplasm containing UEA-I positive, PAS-positive, and PAS-diastase labile materials. Some o ..................More ...
This study explored the daily life physical activity (PA) experiences of 11 adolescent girls living in a rural community in the Northwest of the United States. This qualitative study employed visual methods to explore adolescent girls PA experiences in their daily lives. Specifically, this study used visual diaries and photo-elicitation interviews to capture girls PA experiences. Data from this study revealed two distinct PA patterns among the 11 participants: casual movers and sporty girls. Casual movers have a much less structured approach to PA. They engage in a wide variety of PA types-mostly individual forms of PA and PA geared towards recreation. They describe fun, enjoyment, and task mastery as their main motivations to be physically active. Casual movers often engage in PA with family members and are compelled to be active outdoors and in their homes or neighborhoods. In contrast, all five sporty girls belong to competitive sports teams and have a more structured PA routine. They seek ...
Research into daily physical activity (PA) patterns of older adults (≥70 years) has primarily relied on self-report. This study… Expand ...
Reversine, a purine analog, experienced been proved that it could induce dedifferentiation of differentiated cells into multipotent progenitor cells. acid-Schiff staining assay in hepatogenic differentiated … Manifestation of pluripotent guns and epigenetic guns To further characterize the pluripotency of reversine-pretreated cells, manifestation of specific guns 468-28-0 supplier (April4, Sox2 and Nanog) of pluripotent cells were analyzed by using RT-PCR, western… More →. ...
Rashes from chigger bites are not contagious because the chigger must bite the person for the rash to form. Chiggers are the tiny red larvae of a mite. They are found in wooded areas, tall grass and...
Granular cell tumours (GCTs) have been described in human vocal cords, but not in those of the dog. A single polyp in the left vocal cord, associated with laryngeal oedema and monolateral hyperplasia of the tonsil, was observed endoscopically in a 6-year-old male dog. Cytologically, the mass was characterized by well-defined polygonal cells, with granular cytoplasm and an eccentric nucleus. Histologically, the mass showed sheets of globoid to polygonal cells, filled with numerous diastase-resistant, periodic acid-Schiff-positive granules, and scanty stroma. Ultrastructurally, the granules were represented by heterogeneous lysosomes of variable size, suggesting a diagnosis of GCT. As reported in man, hyperplasia of the laryngeal and tonsillar epithelium and the presence of foci of pearl-like epithelial cells, mimicking invasive squamous cell carcinoma, were observed. Immunohistochemical examination revealed labelling of neoplastic cells for S-100 protein, CD68, collagen IV, and, focally, for ...