Retrograde perfusion (retroperfusion) is an artificial method of providing blood supply to an organ by delivering oxygenated blood through the veins. It may be performed during surgery that interrupts the normal arterial supply of blood to that organ. For instance, when performing surgery that interrupts the cerebral arteries, a hose placed into the femoral artery and the superior vena cava can redirect blood up the internal jugular vein to supply the brain. This technique was pioneered by Oscar Langendorff, who perfused mamallian hearts ex vivo for research applications. Thus, it is often called Langendorff perfusion. Langendorff heart Perfusion Levy WJ, Levin SK, Bavaria JE (July 1995). Cerebral oxygenation during retrograde perfusion. Ann. Thorac. Surg. 60 (1): 184-6. doi:10.1016/s0003-4975(94)01000-5. PMID 7598586. Bell, R., Mocanu, M. & Yellon, D. Retrograde heart perfusion: The Langendorff technique of isolated heart perfusion. Journal of Molecular and Cellular Cardiology 50, 940-950 ...
Introduction: Increasing the usage of hearts from marginal donors and from donors after circulatory death (DCD) has the potential to expand the donor pool. Such hearts may be resuscitated when subjected to ex vivo perfusion. In addition, this may provide an opportunity for viability testing prior to transplant. We describe a novel ex vivo perfusion system designed to reanimate porcine DCD hearts. The same system was subsequently tested using a human heart from a marginal brainstem-dead donor.. Method: In the first phase of the study 23 porcine hearts were procured following circulatory death. All hearts were subjected to a period of primary warm ischemia followed by 120 minutes of hypothermic preservation. The period of hypothermic preservation was initially static cold storage (SCS); then oxygenated machine perfusion and finally a combination of static cold storage and oxygen persufflation via the coronary sinus. Ex vivo perfusion of the hearts was performed with a normothermic, oxygenated ...
TY - JOUR. T1 - Editorial Comment on. T2 - Intraoperative Retrograde Perfusion Sphincterometry to Evaluate Efficacy of Autologous Vas Deferens 6-Branch Suburethral Sling to Properly Restore Sphincteric Apparatus during Robot-Assisted Radical Prostatectomy by Cestari et al.. AU - Sundaram, Chandru. PY - 2017/9/1. Y1 - 2017/9/1. UR - http://www.scopus.com/inward/record.url?scp=85038619116&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85038619116&partnerID=8YFLogxK. U2 - 10.1089/end.2017.0552. DO - 10.1089/end.2017.0552. M3 - Comment/debate. C2 - 28791870. AN - SCOPUS:85038619116. VL - 31. JO - Journal of Endourology. JF - Journal of Endourology. SN - 0892-7790. IS - 9. ER - ...
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Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber ...
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Kinet JP, Bensinger WI, Balland N, Saint-Remy M, Frankenne F, Hennen G, Mahieu P. Ex vivo perfusion of plasma over protein A columns in human mammary adenocarcinoma. Role of the Fc-binding capacity of protein A in the side effects and the tumoricidal response. Eur J Clin Invest. 1986 Feb; 16(1):50-5 ...
These lungs will be brought to the study transplant center to be re-assessed by the transplant team. The lungs will be physiologically assessed during ex vivo perfusion with Steen Solution. Perfusion of these lungs will be performed using Steen solution with the addition of methylprednisolone, heparin and antibiotics. With respect to the decision of lung utilization those organs with a delta pO2 (Δ pO2 = Pulmonary vein pO2 - pulmonary artery pO2) during ex vivo perfusion assessment , 350mmHg, good lung compliance, and a favorable opinion of the transplant surgeon will be considered transplantable. Lungs will be excluded for transplantation: if the Δ pO2 is less than 350mmHg or if they demonstrate ,10% deterioration in any of the following functional parameters: pulmonary vascular resistance (PVR), dynamic compliance or airway pressures. Lungs will also be excluded if they are deemed unsuitable based on the clinical judgment of the lung transplant surgeon ...
Victims of severe accidental hypothermia are prone to fluid extravasation but rarely develop lung edema. We hypothesize that combined hypothermia-induced increase in pulmonary vascular resistance (PVR) and a concomitant fall in cardiac output protect the lungs against edema development. Our aim was to explore in hypothermic- isolated blood-perfused rat lungs whether perfusion at constant pressure influences fluid filtration differently from perfusion at constant flow ...
The effects of acetylcholine on glucose and lactate balance and on perfusion flow were studied in isolated rat livers perfused simultaneously via the hepatic artery (100 mmHg, 25-35% of flow) and the portal vein (10 mmHg, 75-65% of flow) with a Krebs-Henseleit bicarbonate buffer containing 5 mM-glucose, 2 mM-lactate and 0.2 mM-pyruvate. Arterial acetylcholine (10 microM sinusoidal concentration) caused an increase in glucose and lactate output and a slight decrease in arterial and portal flow. These effects were accompanied by an output of noradrenaline and adrenaline into the hepatic vein. Portal acetylcholine elicited only minor increases in glucose and lactate output, a slight decrease in portal flow and a small increase in arterial flow, and no noradrenaline and adrenaline release. The metabolic and haemodynamic effects of arterial acetylcholine and the output of noradrenaline and adrenaline were strongly inhibited by the muscarinic antagonist atropine (10 microM). The ...
We aimed at reviewing design and realisation of perfusion/flow phantoms for validating quantitative perfusion imaging (PI) applications to encourage best practices. A systematic search was performed on the Scopus database for
WATERS IGL® Pulsatile Perfusion Solution has a calculated potassium concentration of 25 mEq/L, a sodium concentration of 100 mEq/L, an osmolarity of 300 mOsM, and a pH of 7.4 at room temperature. Its ion composition is comparable with that of the intracellular milieu. ...
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TY - GEN. T1 - A steady state perfusion experiment. AU - Karlsen, OT. AU - Creyghton, JHN. AU - Mehlkopf, AF. AU - Bovee, WMJJ. N1 - ISSN 1065-9889. PY - 1996. Y1 - 1996. M3 - Conference contribution. SP - 1308. EP - 1308. BT - Proceedings of the fourth scientific meeting and exhibition of the ISMRM-4. ER - ...
TY - GEN. T1 - Automatic assessment of cardiac perfusion MRI. AU - Ólafsdóttir, Hildur. AU - Stegmann, Mikkel Bille. AU - Larsson, Henrik B.W.. PY - 2004. Y1 - 2004. N2 - In this paper, a method based on Active Appearance Models (AAM) is applied for automatic registration of myocardial perfusion MRI. A semi-quantitative perfusion assessment of the registered image sequences is presented. This includes the formation of perfusion maps for three parameters; maximum up-slope, peak and time-to-peak.. AB - In this paper, a method based on Active Appearance Models (AAM) is applied for automatic registration of myocardial perfusion MRI. A semi-quantitative perfusion assessment of the registered image sequences is presented. This includes the formation of perfusion maps for three parameters; maximum up-slope, peak and time-to-peak.. M3 - Article in proceedings. BT - Medical image computing and computer assisted intervention, MICCAI. PB - Springer. T2 - Medical image computing and computer assisted ...
Intestinal segments (5 ± 1 cm) were everted according to the method described by Wilson & Wiseman. After being everted, segments of guts were blotted with a piece of whatman filter paper no. 40 and weighted. A 1 g glass weight was fixed and tied to the end of the everted gut segment to make an empty gut sac. This was important to prevent peristaltic muscular contractions, which may otherwise alter the shape and internal volume of the sac. The 1 g glass weight was the minimum weight to secure the above-mentioned conditions. After weighing, the empty sac was filled with 0.5 mL of Krebs-Henseleit bicarbonate buffer (KHB). The composition of the buffer was: NaHCO3 25 mM/L; NaCl 118 mM/L; KCl 4.7 mM/L; MgSO4 1.2 mM/L; NaH2PO4 1.2 mM/L; CaCl2 1.2 mM/L; and Na4EDTA 9.7 mg/L. Glucose (2g/L) was added to the medium just before the start of the appropriate experiment. The pH was maintained at 7.4. The sac was filled with a blunted-ended syringe and then the needle was slipped off carefully, and the ...
Methods and apparatus are provided for use in intraoperative and percutaneous procedures for supplying long-term retrograde perfusion of the myocardium via one or more conduits disposed between the left ventricle and the coronary venous vasculature. The conduits are of a selected size and number, and portions of the venous vasculature or coronary ostium are partially or completely occluded, to maintain a parameter related to the pressure attained in the venous vasculature to a value less than a predetermined value.
BACKGROUND: Extracorporeal perfusion (EP) is moving into focus of research in reconstructive and transplantation medicine for the preservation of amputates and free tissue transplants. The idea behind EP is the reduction of ischemia-related cell dama
Position: Associate Director - Cardiovascular Perfusion. Location: Cincinnati, OH. Description: The Department of Cardiovascular Perfusion is seeking an Associate Director - Cardiovascular Perfusion to assist the Director of Cardiovascular Perfusion supervising the application and development of circulation technology techniques in the operating room and throughout the institution. Partners with the Director of Cardiovascular Perfusion and medical team to develop innovative strategies for identified cardiovascular perfusion clinical initiatives. Participates in the development of clinical and research model that elevate the Heart Institute to become nationally and internationally redound cardiovascular perfusion programs. Assists the Director of Cardiovascular Perfusion in the day to day operations of the department. Requirements:. *Bachelors degree required in an allied health field or equivalent with a strong science background, and advanced training in circulation technology from a CAHEP ...
Suitable for 3D tissue cultivation Conventional perfusion methods use basically the same structure as static cultivation and just replace a medium slowly and continuously, where the flow of the medium only passes over the layer of cells. Our new system utilises cell culture inserts and enables 3-dimensional perfusion t
Functional tissues generated under in vitro conditions are urgently needed in biomedical research. However, the engineering of tissues is rather difficult, since their development is influenced by numerous parameters. In consequence, a versatile culture system was developed to respond the unmet needs.Optimal adhesion for cells in this system is reached by the selection of individual biomaterials. To protect cells during handling and culture, the biomaterial is mounted onto a MINUSHEET(R) tissue carrier. While adherence of cells takes place in the static environment of a 24 well culture plate, generation of tissues is accomplished in one of several available perfusion culture containers. In the basic version a continuous flow of always fresh culture medium is provided to the developing tissue. In a gradient perfusion culture container epithelia are exposed to different fluids at the luminal and basal sides. Another special container with a transparent lid and base enables microscopic ...
CoverWell™ Perfusion Chambers are press-to-seal incubation chambers designed to that attach onto wet or dry surfaces without the use of adhesive and ideal for live cell imaging, cell incubations and cytochemistry. Similar to CoverWell Incubation Chambers, these water-tight perfusion chambers are made of medical grade silicone bonded to clear, UV-transparent plastic except with ports for fluid exchange within the chamber. Various configurations were designed to provide single- or multi-well chambers when pressed to coverslips or microscope slides. Reagents can be quickly added and removed through dual access ports without disturbing or cross-contaminating specimens in adjacent wells. Chambers are well suited for live cell imaging on upright or inverted microscopes and are removable for permanent mounting of specimens. They can be washed and reused. Please note, that we can customize chamber shape, size and depth for your application.. ...
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In order to remove tumor necrosis factor α (TNFα) or/and bacterial lipopolysaccharides (LPS, endotoxin) extracorporeally from whole blood or/and blood plasma in an extracorporeal perfusion system, the blood or plasma is passed over a cation exchanger and an anion exchanger material. A device according to the invention for the extracorporeal treatment of patients blood or plasma therefore contains a cation exchanger material and an anion exchanger material wherein these materials are contained in at least one compartment of an extracorporeal perfusion system.
Adopted January, 1993. Preamble: Since 1979, the Academy has served as an educational organization to encourage and stimulate investigation and study. The Academy has continued to support perfusion education with the first perfusion education scholarship program in 1983. In support of perfusion education and the process for recognizing effective perfusion education programs, The Academy recognizes the following ideal characteristics for an effective Perfusion Education Program.. THE EFFECTIVE PERFUSION EDUCATION PROGRAM:. ...
The global Perfusion System Market size is expected to value at USD 183.8 million by 2025. The market is subject to witness a substantial growth due to the rising occurrence of organ failures, and spurring demand for transplantation procedures
Transportable Perfusion System Market Insights (2020 to 2025) - Analysis and Forecast for the Global and Chinese Markets ✅ Investors Diurnal Finance Magazine Your business news source, updated 24/7 ✅ Click here for more news.
The global perfusion systems market is expected to reach US$ 1,442.28 Mn in 2027 from US$ 1,044.75 Mn in 2018. The market is estimated to grow with a CAGR of 3.8% ...
The Medical Perfusion System Market globally is a standout amongst the most emergent and astoundingly approved sectors. This worldwide market has been developing at a ...
Lung perfusion has more than tripled since 2015, growing from 1.7 percent to 6.3 percent and potentially expanding the pool of organs available for transplant.
Although machine perfusion is a hot topic today, we are just at the beginning of understanding the underlying mechanisms of protection. Recently, the first randomized controlled trial reported a significant reduction of ischemic cholangiopathies after transplantation of livers donated after circulatory death, provided the grafts were treated with an endischemic hypothermic oxygenated perfusion (HOPE). This approach has been known for more than fifty years, and was initially mainly used to preserve kidneys before implantation. Today there is an increasing interest in this and other dynamic preservation technologies and various centers have tested different approaches in clinical trials and cohort studies. Based on this, there is a need for uniform perfusion settings (perfusion route and duration), and the development of general guidelines regarding the duration of cold storage in context of the overall donor risk is also required to better compare various trial results. This article will highlight how
Author: Finch, S.A.E. et al.; Genre: Journal Article; Published in Print: 1988-07; Title: A perfusion chamber for high-resolution light microscopy of cultured cells.
Our courses and practice exams are categorized and focused on the 11 major sections (Anatomy & Physiology, Pharmacology, Pathology, Laboratory Analysis, Quality Assurance, Devices & Equipment, Clinical Management, Special Patient Groups, Special Procedures/Special Techniques, Catastrophic Events & Device Failure, Monitoring) of perfusion knowledge base as emphasized by the American Board of Cardiovascular Perfusion, and the cardiovascular perfusion consensus curriculum created and adopted by the Accreditation Committee-Perfusion Education.. ...
Magnetic Particle Imaging of the vasculature to assess blood delivery and tissue level perfusion. The direct quantitation of nanoparticle tracers enables accurate perfusion measurements without confounding background tissue signal. Long circulating tracers persist within the vasculature for days, enabling repeated measurements for longitudinal studies. This enables truly functional imaging to measure changes in blood flow and tracer delivery over time in stroke and tumor models. ...
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Autoregulation of blood flow denotes the intrinsic ability of an organ or a vascular bed to maintain a constant perfusion in the face of blood pressure changes. Alternatively, autoregulation can be defined in terms of vascular resistance changes or simply arteriolar caliber changes as blood pressure
➜ Rhyming Words List for Perfusion - Find all words that rhyme with perfusion at RhymeDB.com. Near Rhymes, Meanings, Similar Endings, Similar Syllables.
Perfusion of neonatal mouse? - posted in Neuroscience: Hi, has anyone done an intracardiac perfusion on an infant mouse? I keep reading papers that just say to do it, but I am not sure how- what gauge needle would I use, what flow rate, how to get the skull off without mangling the brain (the answer to that one may be be very careful...) Thanks, if you have done this, I would appreciate tips -DB
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Hello, are you looking for article Clinical assessment of peripheral perfusion to predict ? If it is true we are very fortunate in being able to provide information Clinical assessment of peripheral perfusion to predict And good article Clinical assessment of peripheral perfusion to predict This could benefit/solution for you. ...
Cardiovascular Perfusion Clinical 4 is a continuation of PERF 8300. The students are expected to improve upon the level of competency gained in previous clinical courses. In this clinical course, students will continue to review and apply the objectives introduced in previous rotations, as well as prepare and apply advanced Perfusion techniques and technologies. Review of CPB case studies will assist the students in understanding various clinical scenarios and conditions that have presented in the past and how they were managed.
The perfusion set enables perfusion applications with 2 inlets and 2 outlets and is compatible to a large variety of 35 Petri dishes.
Eddie Yin Ng, Dhanjoo N Ghista, "Cardiac Perfusion and Pumping Engineering" 2007 | pages: 612 | ISBN: 9812706968 | PDF | 12,7 mb
Organ Assist is a company that develops and produces Organ Perfusion Systems. Our integrated solutions for liver, kidney and lung allow for more and better quality donor organs to become available.
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Hello, Im using a perforated MEA with a 2-way peristaltic pump meaning, that I have a perfusion from the top and one from the bottom. I also use two different
We develop and produce our instruments in Germany. These include a wide range of amplifiers, isolated stimulators, devices for precise substance application, temperature controllers (see our Product Overview).. ...
Testing different conditions while performing live-analyses is a challenge, though key to compare cellullar answer to different stimuli. ...
The APS system comes equipped with filter switching, liquid management, and cell bleed to reduce risk of human error and increase process robustness.
Im not sure Im following your question.. When a drive detects an unwritable, or bad sector, its supposed to mark the unwritable block as bad, then re-map the bad sector to a spare sector. It makes no difference what type of data is contained in the data to be written to the drive. This will happen until the amount of spare sectors are used up. After they are, then with most drives, the bad sector is simply left in place and the drive should be considered bad.. From the post from your Apple link, I would tend to agree more with Martin Joseph than some of the others. If the number of bad sectors is increasing with any degree of regularity, then it likely means bearings are failing in either the actuator or the drive motor. This allows too much play in the mechanisms and results in more and more head crashes as the problem gets worse, which causes more and more bad sectors. Either the drive will fail from mechanical problem (bad bearings,) the heads will eventually become damaged, or the ...