The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene ...
R. Lavery, K. Zakrzewska, D.L. Beveridge, T.C. Bishop, D.A. Case, T.E. Cheatham, III, S. Dixit, B. Jayaram, F. Lankaš, C. Laughton, J.H. Maddocks, A. Michon, R. Osman, M. Orozco, A. Perez, T. Singh, N. Špačková, J. Šponer: A systematic molecular dynamics study of nearest-neighbor effects on base pair and base pair step conformations and fluctuations in B-DNA. Nucleic Acids Research 38, 2010, 299-313 ...
R. Lavery, K. Zakrzewska, D.L. Beveridge, T.C. Bishop, D.A. Case, T.E. Cheatham, III, S. Dixit, B. Jayaram, F. Lankaš, C. Laughton, J.H. Maddocks, A. Michon, R. Osman, M. Orozco, A. Perez, T. Singh, N. Špačková, J. Šponer: A systematic molecular dynamics study of nearest-neighbor effects on base pair and base pair step conformations and fluctuations in B-DNA. Nucleic Acids Research 38, 2010, 299-313. ...
The DNA photoaffinity ligands, 7-azidoactinomycin D and 8-azidoethidium, form DNA adducts that cause chain cleavage upon treatment with piperidine. Chemical DNA sequencing techniques were used to detect covalent binding. The relative preferences for modifications of all possible sites defined by a base pair step (e.g. GC) were determined within all quartet contexts such as (IGCJ). These preferences are described in terms of effective site occupations, which expressthe ability of a ligand to covalently modify some base in the binding site. Ideally, the effective site occupations measured for photoaffinity agents can also be related to site-specific, non-covalent association constants of the ligand. The sites most reactive with 7-azidoactinomycin D were those preferred for non-covalent binding of unsubstituted actinomycin D. GC sites were most reactive, but next-nearest neighbors exerted significant influences on reactivity. GC sites in 5′-(pyrimidine)GC(purine)-3′ contexts, particularly ...
A surgical needle is attached to a spiroid braided suture to provide a combined surgical needle-spiroid braided suture device. A shrinkable tubing is employed to secure the needle to the suture. The tubing shrinks in response to the application of energy, e.g., heat. Attachment parameters may be controlled to provide standard needle attachment or removable needle attachment.
The reaction of the potential anticancer drug kiteplatin, cis-[PtCl2(cis-1,4-DACH)], with oligomers of single- and double-stranded DNA ranging from 2 to 12 base pairs in length was performed as a model for DNA interaction. The potential for conformational flexibility of single-stranded adducts was examined with density functional theory (DFT) and compared with data from 1H-NMR 1D and 2D spectroscopy. This indicates the presence of multiple conformations of an adduct with d(GpG), but only one form of the adduct with d(TGGT). The importance of a suitable theoretical model, and in particular basis set, in reproducing experimental data is demonstrated. The DFT theoretical model was extended to platinated base pair step (GG/CC), allowing a comparison to the related compounds cisplatin and oxaliplatin. Adducts of kiteplatin with larger fragments of double-stranded DNA, including tetramer, octamer, and dodecamer, were studied theoretically using hybrid quantum mechanics/molecular mechanics methods. ...
Complexes of methylene blue with DNA are characterized by time- resolved fluorescence spectroscopy and transient photobleaching methods. At least four, and probably five, spectroscopically distinct binding sites have been identified. Three of these (components 1, 2, and 3B) dominate the fluorescence decay at low ionic strength and have fluorescence lifetimes significantly different from that of the free dye. With increasing ionic strength a fourth component (3A) appears at the expense of components 1 and 3B. Component 3A exhibits two subcomponents with different degrees of shielding from O2 quenching of its triplet state. The relative amplitudes of the components at low ionic strength are strongly dependent on the composition of the DNA, and independent of superhelix density. Hence, it is inferred that components 1, 2, and 3B represent binding to different base pair steps, and that all of these components represent intercalation sites that unwind the DNA to the same degree. Component 3A is ...
Additional engineered enzymes (not included in the present design) may be needed to digest bacteriophages that may be resident inside certain bacteria. To avoid digestion by bacterial restriction enzymes, phages often employ unusual molecular substitutions involving 2,6-diaminopurine, 6-methyladenine, 8-azaguanine, 5-hydroxymethyl uracil, 5-methylcytosine, 5-hydroxymethylcytosine, and others [121]. For example, B. subtilis phage DNA replaces thymine with hydroxymethyluracil and uracil; S-2L cyanophage replaces adenine by 2-aminoadenine (2,6-diaminopurine); SPO1, SP82G, and Phi-e substitute hydroxymethyl dUTP for dTTP in the phage DNA up to 20%; PBS1 and PBS2 phages substitute uracil for thymine; T-even (T2/T4/T6) phage DNA replaces dCMP by hydroxymethylcytosine which is then further glycosylated, rendering the phage DNA resistant to host restriction; and in phage Mu DNA, a unique glycinamide moiety modifies about 15% of the adenine residues [121]. Given our complete future knowledge of phage ...
Additional engineered enzymes (not included in the present design) may be needed to digest bacteriophages that may be resident inside certain bacteria. To avoid digestion by bacterial restriction enzymes, phages often employ unusual molecular substitutions involving 2,6-diaminopurine, 6-methyladenine, 8-azaguanine, 5-hydroxymethyl uracil, 5-methylcytosine, 5-hydroxymethylcytosine, and others [121]. For example, B. subtilis phage DNA replaces thymine with hydroxymethyluracil and uracil; S-2L cyanophage replaces adenine by 2-aminoadenine (2,6-diaminopurine); SPO1, SP82G, and Phi-e substitute hydroxymethyl dUTP for dTTP in the phage DNA up to 20%; PBS1 and PBS2 phages substitute uracil for thymine; T-even (T2/T4/T6) phage DNA replaces dCMP by hydroxymethylcytosine which is then further glycosylated, rendering the phage DNA resistant to host restriction; and in phage Mu DNA, a unique glycinamide moiety modifies about 15% of the adenine residues [121]. Given our complete future knowledge of phage ...
21220-77-9 - JJQPAXLGQXCSHZ-UHFFFAOYSA-N - Ethanethiol, 2-(4-(p-cyclohexylphenoxy)butyl)amino-, hydrogen sulfate (ester) - Similar structures search, synonyms, formulas, resource links, and other chemical information.
T01191 (acav,adh,amin,apom,arn,arx,asoc,ato,bacs,balt,bara,barw,bcae,bko,camg,cmb,def,fln,frm,gli,gtm,lagl,les,lzy,mbov,mee,ntp,ntt,parb,part,pcx,pht,ppoa,ptu,rhu,sbj,sgv,slau,smal,sphd,sscu,sya,tpaf,trl : calculation not yet completed ...
Guanine-rich DNA sequences tend to form four-stranded G-quadruplex structures. Characteristic glycosidic conformational patterns along the G-strands, such as the 5-syn-anti-syn-anti pattern observed with the Oxytricha nova telomeric G-quadruplexes, have been well documented. However, an explanation for these featured glycosidic patterns has not emerged. This work presents MD simulation and free energetic analyses for simplified two-quartet [d(GG)](4) models and suggests that the four base pair step patterns show quite different relative stabilities: syn-anti , anti-anti , anti-syn , syn-syn. This suggests the following rule: when folding, anti-parallel G-quadruplexes tend to maximize the number of syn-anti steps and avoid the unfavorable anti-syn and syn-syn steps. This rule is consistent with most of the anti-parallel G-quadruplex structures in the Protein Databank (PDB). Structural polymorphisms of G-quadruplexes relate to these glycosidic conformational patterns and the lengths of the ...
Abstract. Reduction of ethanethiol esters of α-amino acids to α-amino aldehydes by triethylsilane and catalytic palladium-on-carbon is described. α-Amino aldehydes with Boc, Cbz, or Fmoc protection could be obtained without racemization in high yield.. ...
Active DNA demethylation in mammals. (a) The action of AID on 5-methylcytosine residues (white circles) in DNA (thick black line) gives rise to deaminated 5-met
Alfa Chemistry is the worlds leading provider for special chemicals. We offer qualified products for 53279-73-5((2-HYDROXYMETHYL-4,5-DIIODO-PHENYL)-METHANOL),please inquire us for 53279-73-5((2-HYDROXYMETHYL-4,5-DIIODO-PHENYL)-METHANOL).
5-hmC (5-Hydroxymethylcytosine), DNA pyrimidine nitrogen base (CAS 1123-95-1). Join researchers using our high quality biochemicals.
描述的是一个两步骤的标记过程中使用的β-葡糖基转移酶(β-GT)来传输的叠氮化物5-HMC-葡萄糖,然后通过点击化学生物素链接器为容易和密度独立富集转移。这种高效的和具体的标记方法使富集5 - ...
Alterations in DNA methylation may cause disturbances in regulation of gene expression, including drug metabolism and distribution. Moreover, many cancers, including breast cancer, are characterized by DNA hypomethylation and a decreased 5-hydroxymethylcytosine level. The abnormal cell growth...
Visit ChemVia,you find CAS NO.75507-25-4,2-Hydroxymethyl-15-crown-5 specification and current price;you can buy 75507-25-4,2-Hydroxymethyl-15-crown-5 reagent online,and you can buy 2-Hydroxymethyl-15-crown-5 on bulk underline.
To a solution of 3,5-diphenylpyrazole 1 [1] (200 mg, 0.90 mmol) in ethanol (10 mL) was added formaldehyde 2 (110 mg, 1.28 mmol, 35 %) and the mixture was refluxed for two hours [2,3].[...]
Açıklanan kolay ve yoğunluk bağımsız zenginleştirme için bir biyotin bağlayıcının aktarmak tıkırtı kimya ve ardından, 5-HMC için bir...
This page contains information on the chemical Ethanethiol, 2-(1-adamantyl)amino-, hydrogen phosphate (ester), hydrate including: 3 synonyms/identifiers.
The DNA demethylation pathway has been discovered to play a significant role in DNA epigenetics. This pathway removes the methyl group from cytosine, which is involved in the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine (5-hmC) by ten-eleven translocation (TET) proteins. Then, 5-hmC can be iteratively oxidized to generate 5-formylcytosine (5-foC) and 5-carboxylcytosine (5-caC). However, 5-hmC, 5-foC, and 5-caC are hardly detected due to their low content. In this study, we have developed a LC-HRMS method coupled with derivatization to accurately and simultaneously quantify 5-mC levels, along with its oxidation products in genomic DNA ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Magnesium atom in PDB 3ilo: Crystal Structure of E. Coli Hppk(D97A) in Complex With Mgampcpp and 6-Hydroxymethyl-7,8-Dihydropterin
(2E,6Z)-9-hydroxy-4-hydroxymethyl-2,6-nonadiene: isolated from endophytic fungus S20 of Cephalotaxus hainanensis; structure in first source
Search thousands of sociological books, journal articles, theses and dissertations by subject, author, title or publication information. To begin, enter search term(s) below and click Go! Title links in search results lead to item in Amazon.com.. You can also browse citations by subject by clicking on the "Browse Terms" tabs.. ...
chemBlink provides information about CAS # 100-79-8, Solketal, (+/-)-2,2-Dimethyl-1,3-dioxolane-4-methanol, (+/-)-2,2-Dimethyl-4-hydroxymethyl-1,3-dioxolane, 1,2-Isopropylidene-rac-glycerol, molecular formula: C6H12O3.
Words that start with thymine, Unscrambled words that start with thymine, Words starting with thymine, Words that begin with thymine, Words beginning with thymine, Words with the prefix thymine
The authors report that Hes5, a Notch effector gene, is serially activated by mammalian glial cells missing (Gcm) and later by the canonical Notch pathway. Loss of both Gcm1 and Gcm2 and subsequent lack of Hes5 upregulation in the neuroepithelium leads to impaired induction of neural stem cells. Signaling mediated by Notch receptors is crucial for the development of many organs and the maintenance of various stem cell populations. The activation of Notch signaling is first detectable by the expression of an effector gene, Hes5, in the neuroepithelium of mouse embryos at embryonic day (E) 8.0-8.5, and this activation is indispensable for the generation of neural stem cells. However, the molecular mechanism by which Hes5 expression is initiated in stem-producing cells remains unknown. We found that mammalian Gcm1 and Gcm2 (glial cells missing 1 and 2) are involved in the epigenetic regulation of Hes5 transcription by DNA demethylation independently of DNA replication. Loss of both Gcm genes and subsequent
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
... aims at providing comprehensive data on 3(s)-hydroxymethyl-4-bocmorpholine
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.