MicroRNAs (miRNAs) play essential roles in muscle cell proliferation and differentiation. The muscle-specific miRNAs miR-1 and miR-206 have been shown to regulate muscle development and promote myogenic differentiation; however, it is likely that a number of other miRNAs play important roles in regulating myogenesis as well. microRNA-128 (miR-128) has been reported to be highly expressed in brain and skeletal muscle, and we found that miR-128 is also up-regulated during bovine skeletal muscle satellite cell differentiation using microarray analysis and qRT-PCR. However, little is known about the functions of miR-128 in bovine skeletal muscle satellite cell development. In this study, we investigated the biological functions of miR-128 in bovine skeletal muscle cell development. Using a dual-luciferase reporter assay, we confirmed that miR-128 regulates the Sp1 gene. Over-expression of miR-128 reduced Sp1 protein levels and inhibited muscle satellite cell proliferation and differentiation. ...

TY - JOUR. T1 - Desmin is present in proliferating rat muscle satellite cells but not in bovine muscle satellite cells. AU - Allen, Ronald E.. AU - Rankin, Lucinda L.. AU - Greene, Elizabeth A.. AU - Boxhorn, Linda K.. AU - Johnson, Sally E.. AU - Taylor, Richard G.. AU - Pierce, Paul R.. PY - 1991/12. Y1 - 1991/12. N2 - The presence of desmin was characterized in cultured rat and bovine satellite cells and its potential usefulness as a marker for identifying satellite cells in vitro was evaluated. In primary cultures, positive immunohistochemical staining for desmin and skeletal muscle myosin was observed in rat and bovine myotubes. A small number of mononucleated cells (20% of rat satellite cells and 5% of bovine satellite cells) were myosin‐positive, indicative of post‐mitotic differentiated myocytes. In bovine satellite cell cultures 13% of the mononucleated cells were desmin‐positive, while 84% of the mononucleated cells in rat satellite cell cultures were desmin‐positive. Rat ...

TY - THES. T1 - Evaluation of skeletal muscle satellite cell activity in rodent models depicting muscle hypertrophy and atrophy. AU - Sidique, Idris. N1 - Thesis supervisors: Billeter-Clark, R. and Greenhaff, P.L.. PY - 2013/5. Y1 - 2013/5. N2 - Satellite cells are muscle-specific progenitor cells involved in the routine maintenance of skeletal muscle homeostasis, growth and regeneration. They are activated by various stimuli (myotrauma, growth factors etc), undergo rounds of proliferation as skeletal muscle myoblasts, to differentiate and fuse with each other to generate new myotubes or onto existing myofibres to augment growth or repair damaged fibres. Satellite cells contribute to hypertrophy by facilitating nuclear addition, which maintains contractile protein synthetic capacity. Conversely, during atrophy the dysregulation of satellite cells (e.g., via myogenic suppression), causes an opposing deficit in nuclear supplementation/contractile protein synthesis. The activity status of ...

Abstract. Skeletal muscle stem cells from food-producing animals are of interest to agricultural life scientists seeking to develop a better understanding of the molecular regulation of lean tissue (skeletal muscle protein hypertrophy) and intramuscular fat (marbling) development. Enhanced understanding of muscle stem cell biology and function is essential for developing technologies and strategies to augment the metabolic efficiency and muscle hypertrophy of growing animals potentially leading to greater efficiency and reduced environmental impacts of animal production, while concomitantly improving product uniformity and consumer acceptance and enjoyment of muscle foods.. Keywords: Skeletal muscle stem cells, Satellite cells, Adipocytes, Adipofibroblasts, Embryogenesis, Postnatal myogenesis. ...

Asakura A, Komaki M and Rudnicki M (2001). Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation. Differentiation. 68 (4-5): 245-53. Summary: Muscle satellite cells are believed to represent a committed stem cell population that is responsible for the postnatal growth and regeneration of skeletal muscle. However, the observation that cultured myoblasts differentiate into osteocytes or adipocytes following treatment with bone

Sex steroids are known to be involved in skeletal muscle development (anabolic effect) and are frequently used in medicines. It has been known that pork contains a variety of steroids that are mainly synthesized in the gonads (testis and ovary). Thus, the present study was conducted to evaluate the effects of anabolic steroids of pork on the proliferation and differentiation of myogenic satellite cells (MSC). Three different methods (M1, M2, and M3) were developed for the isolation and purification of steroids from porcine tissues. Among three extraction methods that we developed, M3 was the best method with respect to the quantities of steroids and the induction of MSC proliferation. Hormonal analysis showed that the steroid hormone levels were the highest in muscle and fat of intact male than those of castrated males and females. In addition, the highest serum levels of nandrolone and testosterone were detected in intact males, whereas estrone and 17 beta-estradiol levels were similar in the ...

Scientists have found that genes important for forming muscle cells in embryos and newborns are not needed in adult muscle stem cells to regenerate muscles after injury.

Brown adipose tissue (BAT) is an energy-dispensing thermogenic tissue that plays an important role in balancing energy metabolism. Lineage-tracing experiments indicate that brown adipocytes are derived from myogenic progenitors during embryonic development. However, adult skeletal muscle stem cells (satellite cells) have long been considered uniformly determined toward the myogenic lineage. Here, we report that adult satellite cells give rise to brown adipocytes and that microRNA-133 regulates the choice between myogenic and brown adipose determination by targeting the 3UTR of Prdm16. Antagonism of microRNA-133 during muscle regeneration increases uncoupled respiration, glucose uptake, and thermogenesis in local treated muscle and augments whole-body energy expenditure, improves glucose tolerance, and impedes the development of diet-induced obesity. Finally, we demonstrate that miR-133 levels are downregulated in mice exposed to cold, resulting in de novo generation of satellite cell-derived ...

A recent study by Rudnicki and colleagues has sought to address the question of satellite-cell heterogeneity directly (Kuang et al., 2007). The authors have shown that ∼90% of satellite cells on myofibres of adult Myf5cre/+ mice had had a `myogenic experience and expressed Myf5 at some point (as shown by the presence of YFP from the recombined targeted ROSA locus). The remaining ∼10% of satellite cells were YFP-negative, and were able to produce further YFP-negative and YFP-positive cells both in vitro and in vivo. When grafted into Pax7-null mice, these YFP-negative cells gave rise to approximately three times more Pax7-positive satellite cells than the YFP-positive cells, and a quarter of these remained YFP-negative. It was proposed by the authors that these YFP-negative cells correspond to a dedicated subset of satellite cells that have more stem-cell-like characteristics (satellite `stem cells), and that the YFP-positive cells are their transit-amplifying progeny that can undergo ...

Skeletal muscle stem cells from food-producing animals are of interest to agricultural life scientists seeking to develop a better understanding of the molecular regulation of lean tissue (skeletal muscle protein hypertrophy) and intramuscular fat (marbling) development. Enhanced understanding of muscle stem cell biology and function is essential for developing technologies and strategies to augment the metabolic efficiency and muscle hypertrophy of growing animals potentially leading to greater efficiency and reduced environmental impacts of animal production, while concomitantly improving product uniformity and consumer acceptance and enjoyment of muscle foods ...

def: Any process that stops, prevents or reduces the frequency, rate or extent of satellite cell proliferation. [GO_REF:0000058, GOC:TermGenie, PMID:23212449 ...

Satellite cells are self-renewing muscle stem cells, which mediate the growth and repair of skeletal muscle (Zammit et al., 2006). When the muscle is damaged, these cells leave their state of quiescence, proliferate at length to generate colonies of myoblasts, and then differentiate to form multinucleated myotubes (Zammit et al., 2006). The myogenic factor that is mainly involved in controlling the progression of these myoblasts into the differentiation state is MyoD (Le Grand and Rudnicki, 2007). This protein, which preferentially binds to a DNA consensus site (CANNTG) called an E-box, has been shown in both myogenic (C2C12) and nonmyogenic cell lines to be highly important in controlling the transcription of differentiation-specific genes (Berkes and Tapscott, 2005). This function correlates in part to the ability of MyoD to recruit histone acetyltransferases (e.g., PCAF and p300) and SWI/SNF chromatin-remodeling complexes to the promoters of these genes to affect specific changes in chromatin ...

EuroStemCell researchers from the Institut Pasteur have isolated muscle stem cells displaying a high potential for muscle repair. These...

TY - JOUR. T1 - Common and Distinctive Functions of the Hippo Effectors Taz and Yap in Skeletal Muscle Stem Cell Function. AU - Sun, Congshan. AU - De Mello, Vanessa. AU - Mohamed, Abdalla. AU - Quiroga, Huascar P. Ortuste AU - Garcia-Munoz, Amaya AU - Al Bloshi, Abdullah AU - Tremblay, Annie M.. AU - von Kriegsheim, Alexander AU - Collie-Duguid, Elaina. AU - Vargesson, Neil. AU - Matallanas, David. AU - Wackerhage, Henning. AU - Zammit, Peter S.. N1 - Funded by MRC grant G11001931 to HW and PSZ, European Unions Seventh Framework Programme for research, technological development and demonstration under grant agreement number 262948-2 (BIODESIGN), with additional support from AssociationFrançaise Contre les Myopathies, BBSRC, Sarcoma UK and Friends of Anchor.. PY - 2017/8. Y1 - 2017/8. N2 - Hippo pathway downstream effectors Yap and Taz play key roles in cell proliferation and regeneration, regulating gene expression especially via Tead transcription factors. To investigate their role in ...

The capacity of stem cells to generate distinct classes of committed progeny spatiotemporally, although well investigated in the nervous system, remains largely undefined for most tissues and organs. Key to understanding this process is interpreting the relative roles of powerful signalling pathways that impinge on these events. Using different combinations of genetically modified mice, we identify Notch signalling as necessary and sufficient for maintaining muscle stem cells and allowing them to be receptive to specification and differentiation cues throughout development. Remarkably, cell-autonomous expression of the intracellular fragment of Notch in muscle stem/progenitors was sufficient to allow their maintenance, temporal specification and recapitulation of ontology in the absence of committed progeny and differentiation.. We demonstrate that myoblasts with high Notch activity fail to terminally differentiate, both in the embryonic and foetal trunk and head. This property of canonical ...

Adult muscle stem cells are used as a model system to show that the microRNA pathway, and specifically miR-489, is essential for the maintenance of the quiescent state of an adult stem-cell population by suppressing a key proliferation factor, Dek. Little is known about the molecular pathways that maintain stem-cell quiescence. The microRNA (miRNA) pathway is known to be essential for stem-cell pluripotency, proliferation and differentiation, and here Thomas Rando and colleagues show that the miRNA pathway actively maintains the quiescent state of an adult stem-cell population. Using adult-mouse muscle stem cells as a model system, they found that miR-489 is expressed in stem cells, and that the knockdown of miR-489 is sufficient to induce a quiescent muscle stem cell to break quiescence and enter the cell cycle. The oncogene Dek was identified as a key target of miR-489, providing an indication of the kinds of transcriptional programs involved in maintaining and breaking quiescence. Among the key

A study conducted by Childrens Hospital & Research Center Oakland scientists identifies how skeletal muscle stem cells respond to muscle injury and may be stimulated to improve muscle repair in Duchenne Muscular Dystrophy, a severe inherited disease of muscle that causes weakness, disability and, ultimately, heart and respiratory failure.

Translational control of gene expression is an important regulator of adult stem cell quiescence, activation and self-renewal. In skeletal muscle, quiescent satellite cells maintain low levels of protein synthesis, mediated in part through the phosphorylation of eIF2α (P-eIF2α). Pharmacological inhibition of the eIF2α phosphatase with the small molecule sal003 maintains P-eIF2α and permits the expansion of satellite cells ex vivo. Paradoxically, P-eIF2α also increases the translation of specific mRNAs, which is mediated by P-eIF2α-dependent read-through of inhibitory upstream open reading frames (uORFs). Here, we ask whether P-eIF2α-dependent mRNA translation enables expansion of satellite cells. Using transcriptomic and proteomic analyses, we show a number of genes associated with the assembly of the spindle pole to be upregulated at the level of protein, without corresponding change in mRNA levels, in satellite cells expanded in the presence of sal003. We show that uORFs in the 5′ UTR ...

This study demonstrated a number of novel findings regarding satellite cell proliferation in the skeletal muscles of the OZR model of metabolic syndrome. First, there was a decrease in the number of myonuclei in muscle fiber cross sections of the OZR compared with the LZR. Second, this study is the first to show that hypertrophy is not impaired in muscles of the OZR model of metabolic syndrome, if the stimulus is adequate. In addition, to the best of our knowledge, this is the first study to observe any form of loading-induced hypertrophy in any rodent model of metabolic syndrome. The third novel finding is that, although there is no change in the proportion of quiescent satellite cells in control muscles, there was a significant attenuation of satellite cell proliferation of adult muscle under normal control loading conditions in the OZR compared with the LZR. The attenuation of satellite cell proliferation in control soleus and plantaris muscles that we observed in this study may result in a ...

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Muscle dysfunction from disease, trauma or aging, a major health care burden not adequately alleviated by available therapies, could be greatly reduced by treatments that directly address regeneration pathophysiology by capitalizing on muscle stem cells. Muscle stem cells are a subpopulation of incompletely understood muscle progenitors called satellite cells that are focal points of vulnerability in muscle aging and disease states, and in turn, promising therapeutic targets. The proposed research will result in a deeper understanding of the populations of human satellite cells that exist naturally in a broad age range of adults, a necessary foundation for developing muscle stem cell therapeutic applications ...

So, actually, Steve and Jim: youre both right ;) . Aging is not an unitary process: it is the accumulation over the lifetime of multiple forms of cellular and molecular damage. On the one hand, some muscle cells - including, it appears, some cells that would otherwise be satellite cells (skeletal muscle stem cells) - become senescent over the course of life, at least in mice (no studies in humans of which Im aware). We know that these cells exert deleterious effects just like other senescent cells: both Kirklands earlier study in the BubR1-hypotrophic mice and the newer study in mice with irradiated hindlegs subsequently administered senolytics found that clearing these cells improved muscle quality and exercise performance on the treadmill test. On the other hand, it is also true that (of course) there remain functional and apparently largely undamaged satellite cells in aging tissues that none the less remain quiescent in the face of injury due to the baleful influence of the aging ...

Video articles in JoVE about syndecan 3 include Isolation, Culture, and Transplantation of Muscle Satellite Cells, Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans, Minimally Invasive Muscle Embedding (MIME) - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis.

Tichy, E.D., Sidibe, D.K., Tierney, M.T., Stec, M.J., Sharifi-Sanjani, M., Hosalkar, H., Mubarak, S., Johnson, F.B., Sacco, A., Mourkioti, F.: Single Stem Cell Imaging and Analysis Reveals Telomere Length Differences in Diseased Human and Mouse Skeletal Muscles Stem Cell Reports Sept 2017 Notes: This paper was highlighted in Penn Medicine News at: https://www.pennmedicine.org/news/news-releases/2017/september/researchers-find-shortened-telomeres-linked-to-dysfunction-in-duchenne-muscular-dystrophy ...

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Skeletal muscle satellite cells (SCs) are involved in remodeling and hypertrophy processes of skeletal muscle. However, little knowledge exists on extrinsic factors that influence the content of SCs in skeletal muscle. In a comparative human study, we investigated the muscle fiber type-specific association between emergence of satellite cells (SCs), muscle growth, and remodeling in response to 12 wk unilateral resistance training performed as eccentric (Ecc) or concentric (Conc) resistance training ± whey protein (Whey, 19.5 g protein + 19.5 g glucose) or placebo (Placebo, 39 g glucose) supplementation. Muscle biopsies (vastus lateralis) were analyzed for fiber type-specific SCs, myonuclei, and fiber cross-sectional area (CSA). Following training, SCs increased with Conc in both type I and type II fibers (P , 0.01) and exhibited a group difference from Ecc (P , 0.05), which did not increase. Myonuclei content in type I fibers increased in all groups (P , 0.01), while a specific accretion of ...

Skeletal muscle satellite cells located between the plasma membrane and the basal lamina of muscle fibres, could for many years, only be studied in situ by electron microscopy. The introduction of immunohistochemistry and the discovery of molecular markers of satellite cells then made them accessible for light microscopic studies and a wealth of information is today available. Satellite cells are myogenic stem cells that can be activated from a quiescent state to proliferate for self-renewal or differentiate into myogenic cells. The satellite cells are involved in muscle growth during fetal and postnatal development and play a key role in repair and regeneration of damaged muscle fibres. The satellite cells are also essential for muscle fibre hypertrophy and maintenance of muscle mass in the adult. When the present thesis was initiated, studies on satellite cells in human skeletal muscle relied on the neuronal cell adhesion molecule (NCAM) as a marker for satellite cell identification. The ...

Diet-induced obesity impairs muscle satellite cell activation and muscle repair through alterations in hepatocyte growth factor signaling Academic Article ...

BACKGROUND: Amyotrophic lateral sclerosis (ALS) is the most common adult-onset neurodegenerative disease characterized by ascending muscle weakness, atrophy

Science Daily Distinct differences found in how skeletal muscle stem cells cope with different stimuli Date: December 14, 2017 Source: Sanford-Burnham Prebys Medical Discovery Institute A new study led by researchers at Sanford Burnham Prebys Medical Discovery Institute (SBP) describes the biology behind why muscle stem cells respond differently to aging or injury. The findings, published in Cell Stem Cell, have important implications for therapeutic strategies to regenerate skeletal

The mammalian target of rapamycin complex 2 (mTORC2), containing the essential protein rictor, regulates cellular metabolism and cytoskeletal organization by phosphorylating protein kinases, such as PKB/Akt, PKC, and SGK. Inactivation of mTORC2 signaling in adult skeletal muscle affects its metabolism, but not muscle morphology and function. However, the role of mTORC2 in adult muscle stem cells (MuSCs) has not been investigated. Using histological, biochemical, and molecular biological methods, we characterized the muscle phenotype of mice depleted for rictor in the Myf5-lineage (RImyfKO) and of mice depleted for rictor in skeletal muscle fibers (RImKO). The proliferative and myogenic potential of MuSCs was analyzed upon cardiotoxin-induced injury in vivo and in isolated myofibers in vitro. Skeletal muscle of young and 14-month-old RImyfKO mice appeared normal in composition and function. MuSCs from young RImyfKO mice exhibited a similar capacity to proliferate, differentiate, and fuse as controls. In

Video articles in JoVE about satellite cells skeletal muscle include Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle, Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans, Isolation, Culture, and Transplantation of Muscle Satellite Cells, Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells, Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle, Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages, Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles, Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles, Minimally Invasive Muscle Embedding (MIME) - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis, Applications of In Vivo Functional

Skeletal muscle stem cells, called satellite cells and defined by the transcription factor PAX7, are responsible for postnatal muscle growth, homeostasis and regeneration. Attempts to utilize the regenerative potential of muscle stem cells for therapeutic purposes so far failed. We previously established the existence of human PAX7-positive cell colonies with high regenerative potential. We now identified PAX7-negative human muscle-derived cell colonies also positive for the myogenic markers desmin and MYF5. These include cells from a patient with a homozygous PAX7 c.86-1G , A mutation (PAX7null). Single cell and bulk transcriptome analysis show high intra- and inter-donor heterogeneity and reveal the endothelial cell marker CLEC14A to be highly expressed in PAX7null cells. All PAX7-negative cell populations, including PAX7null, form myofibers after transplantation into mice, and regenerate muscle after reinjury. Transplanted PAX7neg cells repopulate the satellite cell niche where they ...

Thomas Rando. Its the first time that a small regulatory RNA, called a microRNA, has been implicated in the maintenance of the adult stem cell resting, or quiescent, state.. Although on the surface the quiescent state seems to be relatively static, its quite actively maintained, said Thomas Rando, MD, PhD, professor of neurology and neurological sciences. Weve found that changing the levels of just one specific microRNA in resting muscle stem cells, however, causes them to spring into action.. Rando, who is also the director of Stanfords Glenn Laboratories for the Biology of Aging and the deputy director of the Stanford Center on Longevity, is the senior author of the research, published Feb. 23 in Nature. Postdoctoral scholar Tom Cheung, PhD, is the first author of the study.. Unlike stem cells in the blood or skin, muscle stem cells spend most of their lives nestled in the surrounding tissue. They dont do much most of the time, said Rando. They remain in a quiescent state for most ...

The researchers suggested that the increase in skeletal muscle satellite cell numbers induced by whey protein supplementation may promote more favourable training adaptations over more prolonged periods.​. Other studies have also reported increases in satellite cell numbers in response to protein supplementation and strength training programmes of similar duration. Previous research has also suggested that increases in satellite cell numbers are a necessary precursor to increasing skeletal muscle mass.. However, more research is needed to clarify potential mechanisms and benefits of increased satellite cell numbers.. More data are needed to examine how increasing dietary protein intake mechanistically affects satellite cell turnover and if protein-induced increases in satellite cell number provide any added benefit to resistance-trained individuals (i.e., reducing recovery time between training bouts due to satellite cell-mediated recovery mechanisms),​ wrote the researchers.. The exact ...

Development. 2015 May 1;142(9):1572-1581. Review Dumont NA1, Wang YX2, Rudnicki MA3. Abstract Muscle stem cells, termed satellite cells, are crucial for skeletal muscle growth and regeneration. In healthy adult muscle, satellite cells are quiescent but poised for activation. During muscle regeneration, activated satellite cells transiently re-enter the cell cycle to proliferate and subsequently exit the cell cycle to differentiate or self-renew. Recent studies have demonstrated that satellite cells are heterogeneous and that subpopulations of satellite stem cells are able to perform asymmetric divisions to generate myogenic progenitors or symmetric divisions to expand the satellite cell pool. Thus, a complex balance between extrinsic cues and intrinsic regulatory mechanisms is needed to tightly control satellite cell cycle progression and cell fate determination. Defects in satellite cell regulation or in their niche, as observed in degenerative conditions such as aging, can impair muscle ...

The elderly often suffer from progressive muscle weakness and regenerative failure. We demonstrate that muscle regeneration is impaired with aging owing in part to a cell-autonomous functional decline in skeletal muscle stem cells (MuSCs). Two-thirds of MuSCs from aged mice are intrinsically defecti …

Characterization of skeletal muscle satellite cells metabolism and redox balance: role of aldehydes as metabolic sensors. Scholarships in Brazil Doctorate. Kátia Maria Gomes Andrade. Biological Sciences

Treating heart failure using muscle stem cells taken from the patient may be a viable option. A new study demonstrates its safety in human patients.

Treating heart failure using muscle stem cells taken from the patient may be a viable option. A new study demonstrates its safety in human patients.

Lien vers Pubmed [PMID] - 22159413. J. Cell. Sci. 2011 Dec;124(Pt 23):3980-8. We report expression of Pax3, an important regulator of skeletal muscle stem cell behaviour, in the brachial and femoral arteries of adult mice. In these contractile arteries of the limb, but not in the elastic arteries of the trunk, bands of GFP-positive cells were observed in Pax3(GFP/+) mice. Histological and biochemical examination of the vessels, together with clonal analysis after purification of Pax3-GFP-positive cells by flow cytometry, established their vascular smooth muscle identity. These blood-vessel-derived cells do not respond to inducers of other mesodermal cell types, such as bone, however, they can contribute to muscle fibre formation when co-cultured with skeletal muscle cells. This myogenic conversion depends on the expression of Pax3, but is rare and non-cell autonomous as it requires cell fusion. Myocardin, which promotes acquisition of a mature smooth muscle phenotype in these Pax3-GFP-positive ...

muscle stem cell migration. Stem Cells . Collins-Hooper, H. and Woolley, T. E. and Dyson, L and Patel, A. and Potter, P. and Baker, R. E. and Gaffney, E. A. and Maini, P. K. and Dash, P. R. and Patel, K. (2012) Age-related changes in speed & mechanism of adult skeletal muscle stem cell migration. Stem Cells, 30 (6). pp. 1182-1195. Mcgillen, J B and Martin, N K and Robey, I F and Gaffney, E. A. and Maini, P. K. (2012) Applications of mathematical analysis to tumour acidity modelling. RIMS Kokyuroku Bessatsu, B31 . pp. 31-59. Verhaegen, P D H M and Van Marle, J and Kuehne, A and Schouten, H J and Gaffney, E. A. and Maini, P. K. and Middlelkoop, E and Van Zuijlen, P P M (2012) Collagen bundle morphometry in skin & scar tissue: a novel distance mapping method provides superior measurements compared to Fourier analysis. Journal of Microscopy, 245 (1). pp. 82-89. Woolley, T. E. and Baker, R. E. and Gaffney, E. A. and Maini, P. K. and Seirin Lee, S (2012) Effects of intrinsic stochasticity on delayed ...

muscle stem cell migration. Stem Cells . Collins-Hooper, H. and Woolley, T. E. and Dyson, L and Patel, A. and Potter, P. and Baker, R. E. and Gaffney, E. A. and Maini, P. K. and Dash, P. R. and Patel, K. (2012) Age-related changes in speed & mechanism of adult skeletal muscle stem cell migration. Stem Cells, 30 (6). pp. 1182-1195. Mcgillen, J B and Martin, N K and Robey, I F and Gaffney, E. A. and Maini, P. K. (2012) Applications of mathematical analysis to tumour acidity modelling. RIMS Kokyuroku Bessatsu, B31 . pp. 31-59. Verhaegen, P D H M and Van Marle, J and Kuehne, A and Schouten, H J and Gaffney, E. A. and Maini, P. K. and Middlelkoop, E and Van Zuijlen, P P M (2012) Collagen bundle morphometry in skin & scar tissue: a novel distance mapping method provides superior measurements compared to Fourier analysis. Journal of Microscopy, 245 (1). pp. 82-89. Woolley, T. E. and Baker, R. E. and Gaffney, E. A. and Maini, P. K. and Seirin Lee, S (2012) Effects of intrinsic stochasticity on delayed ...

PubMed journal article: Muscle engraftment of myogenic progenitor cells following intraarterial transplantation. Download Prime PubMed App to iPhone, iPad, or Android

Satellite cells are located near mature muscle fibers in mice and humans and stay in a dormant state until called upon for repair work. In earlier experiments, Rudnicki found that some satellite cells function as stem cells and maintain overall numbers of satellite cells. He distinguished these from other satellite cells, which are in various stages on the road to becoming muscle tissue.. In muscular dystrophy, satellite cells are believed to become depleted quickly because tissue damage places great demands on them for repairs. Enhancing their numbers could slow the process of muscle degeneration, even in the face of disease.. In muscle degenerative diseases, one of the big problems is thought to be that the muscles run out of repair cells, says Paul Muhlrad, a research program coordinator at MDA. Rudnickis laboratory has figured out the biochemical pathways the body uses to maintain the supply.. When the OHRI researchers injected genes for the WNT7a protein into muscle fibers in mice, ...

When muscle tissue experiences trauma or disease, such as muscular dystrophy, stem cells in the muscle known as satellite cells respond to repair and

A major focus of our research has been to understand the molecular regulation of muscle stem cell (satellite cell (SC)) function in homeostasis,...

Image by foundphotoslj via Flickr CHICAGO (Reuters) - A new understanding of the genes that make muscle cells may change the way researchers think about st

PAX8-expressing tumors PO Definition; PAX8 is a paired-box gene important in embryogenesis of the thyroid, Müllerian tract, and renal/upper (...)

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